AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1393280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393280 MtrunA17_Chr5g0397131 89.512 410 35 4 1 406 1 406 0.0 600
Msa1393280 MtrunA17_Chr8g0387621 40.347 404 134 14 1 365 1 336 4.11e-54 183
Msa1393280 MtrunA17_Chr1g0202421 32.642 386 147 18 1 370 1 289 8.27e-38 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393280 AT1G64080.1 40.940 447 163 19 1 387 1 406 3.32e-59 198
Msa1393280 AT5G52870.1 29.596 223 105 6 152 370 93 267 1.59e-18 86.3
Msa1393280 AT5G52870.2 29.596 223 105 6 152 370 93 267 2.23e-18 85.5

Find 99 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGTTCAACTTTCTGATTC+TGG 0.092598 tig0021636:-111880 None:intergenic
CTTGATTTCCTTGACAATTC+TGG 0.186052 tig0021636:-112630 None:intergenic
TCAAGTGACAAGAGACTTTC+AGG 0.210690 tig0021636:+112648 Msa1393280:CDS
AGAGTTGTTTACAAACATTT+AGG 0.218894 tig0021636:+112148 Msa1393280:CDS
TGATTCTTCCTTCACTTTAT+CGG 0.268629 tig0021636:-112881 None:intergenic
TATGCACAAGTATTTGAAAA+TGG 0.291835 tig0021636:+111952 Msa1393280:CDS
GTTGTTGTTGTTGGTTGTTA+TGG 0.308570 tig0021636:-111421 None:intergenic
TTCTCTTTACTATACTATAC+TGG 0.349389 tig0021636:+111166 Msa1393280:five_prime_UTR
CAAGAGTCAGAGTCAGAAAC+AGG 0.350794 tig0021636:+112102 Msa1393280:CDS
AGCTAAAAGCTTCCATTGTT+GGG 0.369587 tig0021636:-111193 None:intergenic
GTGAATTGAGGTTTGTTGTT+TGG 0.372570 tig0021636:-111662 None:intergenic
GAGTTGTTTACAAACATTTA+GGG 0.374177 tig0021636:+112149 Msa1393280:CDS
TGGTGAAACCTCTTTACATA+AGG 0.375753 tig0021636:+111972 Msa1393280:CDS
AAGCTAAAAGCTTCCATTGT+TGG 0.386486 tig0021636:-111194 None:intergenic
AGCGAATCATCACGGCGTTT+CGG 0.387973 tig0021636:-112226 None:intergenic
ACTTTGAATTTGATTCTCTC+TGG 0.392176 tig0021636:-111782 None:intergenic
TTCACCTCTGGATGCATTGA+AGG 0.393929 tig0021636:-112299 None:intergenic
TGAACCATCATTATCACCTT+TGG 0.395194 tig0021636:+111901 Msa1393280:CDS
AAGTTTCGTGTCTTCATGTC+TGG 0.399226 tig0021636:+111710 Msa1393280:CDS
TCTGGTTTGTTTGATATTTG+TGG 0.403273 tig0021636:-111862 None:intergenic
TCACTTCTTTCATTGTTTGA+AGG 0.403278 tig0021636:-111533 None:intergenic
GGTGAAACCTCTTTACATAA+GGG 0.408319 tig0021636:+111973 Msa1393280:CDS
ATTGACGGTGGTGGTGGTGG+TGG 0.410775 tig0021636:-111273 None:intergenic
GGTATTGACGGTGGTGGTGG+TGG 0.421196 tig0021636:-111276 None:intergenic
TTCAGTGAGTGATCCATTGC+GGG 0.421885 tig0021636:+112599 Msa1393280:CDS
CGAGGTGGTGGTGCTGTAGT+TGG 0.425514 tig0021636:+111230 Msa1393280:CDS
GAAGAGCCGTTCCGCGTCTT+CGG 0.426597 tig0021636:+112171 Msa1393280:CDS
GAAACAGGGGAATAATATTC+CGG 0.441776 tig0021636:+112117 Msa1393280:CDS
GGTGGTATTGACGGTGGTGG+TGG 0.442435 tig0021636:-111279 None:intergenic
CAGCAGCAAGATGGAATTCA+AGG 0.444566 tig0021636:+112253 Msa1393280:CDS
CTGTTTCTGACTCTGACTCT+TGG 0.446943 tig0021636:-112101 None:intergenic
CGAGAAACCCTTATGTAAAG+AGG 0.451055 tig0021636:-111980 None:intergenic
GAAAATACAGTGAATATGTT+GGG 0.454276 tig0021636:-111143 None:intergenic
TTGTCGAGAATGAAGGAAGA+AGG 0.462443 tig0021636:-111620 None:intergenic
GAGACTTTCAGGTTGAAGAA+AGG 0.466659 tig0021636:+112659 Msa1393280:exon
GAGAATGGTGGTGGTATTGA+CGG 0.469232 tig0021636:-111288 None:intergenic
AACAATGAAGCAGTGAATTG+AGG 0.470671 tig0021636:-111674 None:intergenic
TTGTTGTTGGTTGTTATGGT+TGG 0.471851 tig0021636:-111417 None:intergenic
GAGTAGAACTGTTGAGATGA+AGG 0.471893 tig0021636:+112781 Msa1393280:three_prime_UTR
TCTTCCAAAGGTGATAATGA+TGG 0.471973 tig0021636:-111905 None:intergenic
CCGTTCCGCGTCTTCGGCAG+TGG 0.475358 tig0021636:+112177 Msa1393280:CDS
GGCAGTGGCGGCTTCTCCGC+CGG 0.476606 tig0021636:+112192 Msa1393280:CDS
CTTAAATACTGGCGAGGTGG+TGG 0.477962 tig0021636:+111218 Msa1393280:CDS
TCAGAGGATTTATTCTTCAA+AGG 0.479259 tig0021636:+111578 Msa1393280:CDS
AGAAAATACAGTGAATATGT+TGG 0.488295 tig0021636:-111144 None:intergenic
TAAATCAAGCCCGGGTGAAA+AGG 0.488748 tig0021636:+112021 Msa1393280:CDS
TCTTTCACTGTCACCTTCAG+AGG 0.498693 tig0021636:+111562 Msa1393280:CDS
GCTTTCAGCGGTGGTGAGAA+TGG 0.502612 tig0021636:-111303 None:intergenic
GTGGTGGTGGAGTCAGAAGA+AGG 0.503814 tig0021636:-111260 None:intergenic
GTGGTGAAGTGAGAAGAAAA+AGG 0.506170 tig0021636:-111118 None:intergenic
CCATGGTGATAAATCAAGCC+CGG 0.510297 tig0021636:+112012 Msa1393280:CDS
GTAAACTCTAAGTCAAAGAA+AGG 0.510667 tig0021636:-111350 None:intergenic
CAGGGGAATAATATTCCGGC+AGG 0.511050 tig0021636:+112121 Msa1393280:CDS
AGACATGAAGACACGAAACT+TGG 0.531689 tig0021636:-111708 None:intergenic
AGCCGCCACTGCCGAAGACG+CGG 0.542768 tig0021636:-112182 None:intergenic
TCTGGTGAAATTTCCCGCAA+TGG 0.548892 tig0021636:-112612 None:intergenic
TAAACAACTCTTAGCCCTGC+CGG 0.558745 tig0021636:-112136 None:intergenic
GTCGTCGCTGCTTTCAGCGG+TGG 0.560517 tig0021636:-111312 None:intergenic
GTTGTTGTTGTCGAGAATGA+AGG 0.560905 tig0021636:-111627 None:intergenic
TTCCGCGTCTTCGGCAGTGG+CGG 0.563880 tig0021636:+112180 Msa1393280:CDS
CCACTGCCGAAGACGCGGAA+CGG 0.563993 tig0021636:-112177 None:intergenic
AATGCAATAGTGAGTGAAGA+GGG 0.564305 tig0021636:-111051 None:intergenic
TCAAAGGAGACAGTAATGGA+TGG 0.572026 tig0021636:+112743 Msa1393280:three_prime_UTR
TCTTCTTTCCGATAAAGTGA+AGG 0.573975 tig0021636:+112873 Msa1393280:three_prime_UTR
TAGTGGAGAGAAATAGTCAA+AGG 0.582251 tig0021636:+112727 Msa1393280:three_prime_UTR
TTGAAGAATAAATCCTCTGA+AGG 0.584940 tig0021636:-111575 None:intergenic
GATAGTGAAGATGATGATGA+TGG 0.588770 tig0021636:+111473 Msa1393280:CDS
AAATTTCACCAGAATTGTCA+AGG 0.590930 tig0021636:+112622 Msa1393280:CDS
ATAGTCAAAGGAGACAGTAA+TGG 0.591385 tig0021636:+112739 Msa1393280:three_prime_UTR
CAAAGGAGACAGTAATGGAT+GGG 0.594744 tig0021636:+112744 Msa1393280:three_prime_UTR
TTTACATAAGGGTTTCTCGT+AGG 0.595831 tig0021636:+111984 Msa1393280:CDS
AAGAGTCAGAGTCAGAAACA+GGG 0.601317 tig0021636:+112103 Msa1393280:CDS
GAATGCAATAGTGAGTGAAG+AGG 0.602687 tig0021636:-111052 None:intergenic
CATGGTGATAAATCAAGCCC+GGG 0.603985 tig0021636:+112013 Msa1393280:CDS
GGTGGTGGTATTGACGGTGG+TGG 0.606414 tig0021636:-111282 None:intergenic
CTGGTGAAAAGTGAAACAAT+GGG 0.611998 tig0021636:-111815 None:intergenic
TATACTGGCTCTCCCAACAA+TGG 0.616878 tig0021636:+111181 Msa1393280:exon
AGCGACGACAATGATGATGA+AGG 0.617421 tig0021636:+111326 Msa1393280:CDS
TCTGGTGAAAAGTGAAACAA+TGG 0.618237 tig0021636:-111816 None:intergenic
AGCGGTGGTGAGAATGGTGG+TGG 0.618375 tig0021636:-111297 None:intergenic
AATGGTGGTGGTATTGACGG+TGG 0.624051 tig0021636:-111285 None:intergenic
ATTGTCGTCGCTGCTTTCAG+CGG 0.629569 tig0021636:-111315 None:intergenic
AGAGTCAGAGTCAGAAACAG+GGG 0.634295 tig0021636:+112104 Msa1393280:CDS
GCTGCAAAAGCGAATCATCA+CGG 0.637302 tig0021636:-112234 None:intergenic
CGTTTCGGCGACACTAACAC+CGG 0.647872 tig0021636:-112211 None:intergenic
GAAGCAGAAATAGCGACGGA+AGG 0.656677 tig0021636:+112046 Msa1393280:CDS
AGTGAAGATGATGATGATGG+TGG 0.659705 tig0021636:+111476 Msa1393280:CDS
CCGGGCTTGATTTATCACCA+TGG 0.662133 tig0021636:-112012 None:intergenic
AATGGATCACTCACTGAACG+TGG 0.662626 tig0021636:-112594 None:intergenic
GTTTCTCGTAGGTACAGCCA+TGG 0.666123 tig0021636:+111995 Msa1393280:CDS
ACAGTGAATATGTTGGGCAG+TGG 0.667153 tig0021636:-111137 None:intergenic
AGCTTGCTTAAATACTGGCG+AGG 0.670456 tig0021636:+111212 Msa1393280:CDS
GTTCAGTGAGTGATCCATTG+CGG 0.671555 tig0021636:+112598 Msa1393280:CDS
AGATCCTTCAATGCATCCAG+AGG 0.680000 tig0021636:+112295 Msa1393280:CDS
TTGCTTAAATACTGGCGAGG+TGG 0.681386 tig0021636:+111215 Msa1393280:CDS
GGTTGAAGCAGAAATAGCGA+CGG 0.682979 tig0021636:+112042 Msa1393280:CDS
TTCGGCGACACTAACACCGG+CGG 0.685515 tig0021636:-112208 None:intergenic
AGGGGAATAATATTCCGGCA+GGG 0.693221 tig0021636:+112122 Msa1393280:CDS
TTCAGCGGTGGTGAGAATGG+TGG 0.706931 tig0021636:-111300 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTATGAATGAATATTTTTT+TGG - tig0021636:111083-111102 None:intergenic 15.0%
!! TAAAAAATGGAATAATTTAG+AGG - tig0021636:112350-112369 None:intergenic 15.0%
!! AATAAAGACTACATAAAAAA+TGG - tig0021636:112363-112382 None:intergenic 15.0%
!!! TTAATTAATTTTTGTGTCAA+TGG + tig0021636:112509-112528 Msa1393280:intron 15.0%
!!! AGTATGAATGAATATTTTTT+TGG - tig0021636:111083-111102 None:intergenic 15.0%
!! TAAAAAATGGAATAATTTAG+AGG - tig0021636:112350-112369 None:intergenic 15.0%
!! AATAAAGACTACATAAAAAA+TGG - tig0021636:112363-112382 None:intergenic 15.0%
!!! TTAATTAATTTTTGTGTCAA+TGG + tig0021636:112509-112528 Msa1393280:intron 15.0%
!!! AATGAATATTTTTTTGGAAG+GGG - tig0021636:111077-111096 None:intergenic 20.0%
!!! GAATGAATATTTTTTTGGAA+GGG - tig0021636:111078-111097 None:intergenic 20.0%
!!! TGAATGAATATTTTTTTGGA+AGG - tig0021636:111079-111098 None:intergenic 20.0%
!!! GATTTAATTGATGATAGTTT+AGG + tig0021636:112435-112454 Msa1393280:intron 20.0%
!!! AATGAATATTTTTTTGGAAG+GGG - tig0021636:111077-111096 None:intergenic 20.0%
!!! GAATGAATATTTTTTTGGAA+GGG - tig0021636:111078-111097 None:intergenic 20.0%
!!! TGAATGAATATTTTTTTGGA+AGG - tig0021636:111079-111098 None:intergenic 20.0%
!!! GATTTAATTGATGATAGTTT+AGG + tig0021636:112435-112454 Msa1393280:intron 20.0%
! GAAAATACAGTGAATATGTT+GGG - tig0021636:111146-111165 None:intergenic 25.0%
! AGAAAATACAGTGAATATGT+TGG - tig0021636:111147-111166 None:intergenic 25.0%
! TTCTCTTTACTATACTATAC+TGG + tig0021636:111166-111185 Msa1393280:five_prime_UTR 25.0%
! TATGCACAAGTATTTGAAAA+TGG + tig0021636:111952-111971 Msa1393280:CDS 25.0%
! AGAGTTGTTTACAAACATTT+AGG + tig0021636:112148-112167 Msa1393280:CDS 25.0%
! GAGTTGTTTACAAACATTTA+GGG + tig0021636:112149-112168 Msa1393280:CDS 25.0%
! TAATTTAGAGGCTAAATTGA+TGG - tig0021636:112338-112357 None:intergenic 25.0%
! GAAAATACAGTGAATATGTT+GGG - tig0021636:111146-111165 None:intergenic 25.0%
! AGAAAATACAGTGAATATGT+TGG - tig0021636:111147-111166 None:intergenic 25.0%
! TTCTCTTTACTATACTATAC+TGG + tig0021636:111166-111185 Msa1393280:five_prime_UTR 25.0%
! TATGCACAAGTATTTGAAAA+TGG + tig0021636:111952-111971 Msa1393280:CDS 25.0%
! AGAGTTGTTTACAAACATTT+AGG + tig0021636:112148-112167 Msa1393280:CDS 25.0%
! GAGTTGTTTACAAACATTTA+GGG + tig0021636:112149-112168 Msa1393280:CDS 25.0%
! TAATTTAGAGGCTAAATTGA+TGG - tig0021636:112338-112357 None:intergenic 25.0%
!!! CTTTTAGCTTGCTTAAATAC+TGG + tig0021636:111207-111226 Msa1393280:CDS 30.0%
GTAAACTCTAAGTCAAAGAA+AGG - tig0021636:111353-111372 None:intergenic 30.0%
AAGAAGAAGAAGAAGAAGAT+GGG + tig0021636:111384-111403 Msa1393280:CDS 30.0%
TCACTTCTTTCATTGTTTGA+AGG - tig0021636:111536-111555 None:intergenic 30.0%
TTGAAGAATAAATCCTCTGA+AGG - tig0021636:111578-111597 None:intergenic 30.0%
TCAGAGGATTTATTCTTCAA+AGG + tig0021636:111578-111597 Msa1393280:CDS 30.0%
!! CTTCTTTTCAAGAACAGAAT+CGG - tig0021636:111753-111772 None:intergenic 30.0%
ACTTTGAATTTGATTCTCTC+TGG - tig0021636:111785-111804 None:intergenic 30.0%
!!! ATATTTGTGGTTGCTGTTTT+TGG - tig0021636:111852-111871 None:intergenic 30.0%
!! TCTGGTTTGTTTGATATTTG+TGG - tig0021636:111865-111884 None:intergenic 30.0%
!! TTATTCGTTTTATTCACCTC+TGG - tig0021636:112314-112333 None:intergenic 30.0%
AAACCCGTCAATTTAAATTG+AGG + tig0021636:112393-112412 Msa1393280:intron 30.0%
AACCCGTCAATTTAAATTGA+GGG + tig0021636:112394-112413 Msa1393280:intron 30.0%
AGTCCCTCAATTTAAATTGA+CGG - tig0021636:112400-112419 None:intergenic 30.0%
AAATTTCACCAGAATTGTCA+AGG + tig0021636:112622-112641 Msa1393280:CDS 30.0%
AGGTAAACAAAAAAAGAGAG+AGG - tig0021636:112831-112850 None:intergenic 30.0%
!!! CTCTCTTTTTTTGTTTACCT+TGG + tig0021636:112831-112850 Msa1393280:three_prime_UTR 30.0%
! TTTACAGTTTTACACTACCA+AGG - tig0021636:112851-112870 None:intergenic 30.0%
TGATTCTTCCTTCACTTTAT+CGG - tig0021636:112884-112903 None:intergenic 30.0%
!!! CTTTTAGCTTGCTTAAATAC+TGG + tig0021636:111207-111226 Msa1393280:CDS 30.0%
GTAAACTCTAAGTCAAAGAA+AGG - tig0021636:111353-111372 None:intergenic 30.0%
AAGAAGAAGAAGAAGAAGAT+GGG + tig0021636:111384-111403 Msa1393280:CDS 30.0%
TCACTTCTTTCATTGTTTGA+AGG - tig0021636:111536-111555 None:intergenic 30.0%
TTGAAGAATAAATCCTCTGA+AGG - tig0021636:111578-111597 None:intergenic 30.0%
TCAGAGGATTTATTCTTCAA+AGG + tig0021636:111578-111597 Msa1393280:CDS 30.0%
!! CTTCTTTTCAAGAACAGAAT+CGG - tig0021636:111753-111772 None:intergenic 30.0%
ACTTTGAATTTGATTCTCTC+TGG - tig0021636:111785-111804 None:intergenic 30.0%
!!! ATATTTGTGGTTGCTGTTTT+TGG - tig0021636:111852-111871 None:intergenic 30.0%
!! TCTGGTTTGTTTGATATTTG+TGG - tig0021636:111865-111884 None:intergenic 30.0%
!! TTATTCGTTTTATTCACCTC+TGG - tig0021636:112314-112333 None:intergenic 30.0%
AAACCCGTCAATTTAAATTG+AGG + tig0021636:112393-112412 Msa1393280:intron 30.0%
AACCCGTCAATTTAAATTGA+GGG + tig0021636:112394-112413 Msa1393280:intron 30.0%
AGTCCCTCAATTTAAATTGA+CGG - tig0021636:112400-112419 None:intergenic 30.0%
AAATTTCACCAGAATTGTCA+AGG + tig0021636:112622-112641 Msa1393280:CDS 30.0%
AGGTAAACAAAAAAAGAGAG+AGG - tig0021636:112831-112850 None:intergenic 30.0%
!!! CTCTCTTTTTTTGTTTACCT+TGG + tig0021636:112831-112850 Msa1393280:three_prime_UTR 30.0%
! TTTACAGTTTTACACTACCA+AGG - tig0021636:112851-112870 None:intergenic 30.0%
TGATTCTTCCTTCACTTTAT+CGG - tig0021636:112884-112903 None:intergenic 30.0%
AGCTAAAAGCTTCCATTGTT+GGG - tig0021636:111196-111215 None:intergenic 35.0%
AAGCTAAAAGCTTCCATTGT+TGG - tig0021636:111197-111216 None:intergenic 35.0%
TCTTCTTCTTCTTCTTCTTC+AGG - tig0021636:111380-111399 None:intergenic 35.0%
GAAGAAGAAGAAGAAGAAGA+TGG + tig0021636:111380-111399 Msa1393280:CDS 35.0%
! TTGTTGTTGGTTGTTATGGT+TGG - tig0021636:111420-111439 None:intergenic 35.0%
! GTTGTTGTTGTTGGTTGTTA+TGG - tig0021636:111424-111443 None:intergenic 35.0%
!! GTTCTTGTTGTTGTTGTTGT+TGG - tig0021636:111433-111452 None:intergenic 35.0%
! GATAGTGAAGATGATGATGA+TGG + tig0021636:111473-111492 Msa1393280:CDS 35.0%
!! GTGAATTGAGGTTTGTTGTT+TGG - tig0021636:111665-111684 None:intergenic 35.0%
AACAATGAAGCAGTGAATTG+AGG - tig0021636:111677-111696 None:intergenic 35.0%
!! TCAAGAACAGAATCGGTTTT+TGG - tig0021636:111746-111765 None:intergenic 35.0%
CTGGTGAAAAGTGAAACAAT+GGG - tig0021636:111818-111837 None:intergenic 35.0%
TCTGGTGAAAAGTGAAACAA+TGG - tig0021636:111819-111838 None:intergenic 35.0%
CATGTTCAACTTTCTGATTC+TGG - tig0021636:111883-111902 None:intergenic 35.0%
TGAACCATCATTATCACCTT+TGG + tig0021636:111901-111920 Msa1393280:CDS 35.0%
! TCTTCCAAAGGTGATAATGA+TGG - tig0021636:111908-111927 None:intergenic 35.0%
GAAAAACGTTTCTCTTCCAA+AGG - tig0021636:111920-111939 None:intergenic 35.0%
!! GGAAGAGAAACGTTTTTCAA+AGG + tig0021636:111922-111941 Msa1393280:CDS 35.0%
TGGTGAAACCTCTTTACATA+AGG + tig0021636:111972-111991 Msa1393280:CDS 35.0%
GGTGAAACCTCTTTACATAA+GGG + tig0021636:111973-111992 Msa1393280:CDS 35.0%
TTTACATAAGGGTTTCTCGT+AGG + tig0021636:111984-112003 Msa1393280:CDS 35.0%
! GTTTTCTTCACTTTCAGCTA+CGG - tig0021636:112077-112096 None:intergenic 35.0%
GAAACAGGGGAATAATATTC+CGG + tig0021636:112117-112136 Msa1393280:CDS 35.0%
GTCCCTCAATTTAAATTGAC+GGG - tig0021636:112399-112418 None:intergenic 35.0%
CTTGATTTCCTTGACAATTC+TGG - tig0021636:112633-112652 None:intergenic 35.0%
! AGATTTTGCACTATGCATAG+TGG + tig0021636:112710-112729 Msa1393280:three_prime_UTR 35.0%
TAGTGGAGAGAAATAGTCAA+AGG + tig0021636:112727-112746 Msa1393280:three_prime_UTR 35.0%
ATAGTCAAAGGAGACAGTAA+TGG + tig0021636:112739-112758 Msa1393280:three_prime_UTR 35.0%
TCTTCTTTCCGATAAAGTGA+AGG + tig0021636:112873-112892 Msa1393280:three_prime_UTR 35.0%
AGCTAAAAGCTTCCATTGTT+GGG - tig0021636:111196-111215 None:intergenic 35.0%
AAGCTAAAAGCTTCCATTGT+TGG - tig0021636:111197-111216 None:intergenic 35.0%
TCTTCTTCTTCTTCTTCTTC+AGG - tig0021636:111380-111399 None:intergenic 35.0%
GAAGAAGAAGAAGAAGAAGA+TGG + tig0021636:111380-111399 Msa1393280:CDS 35.0%
! TTGTTGTTGGTTGTTATGGT+TGG - tig0021636:111420-111439 None:intergenic 35.0%
! GTTGTTGTTGTTGGTTGTTA+TGG - tig0021636:111424-111443 None:intergenic 35.0%
!! GTTCTTGTTGTTGTTGTTGT+TGG - tig0021636:111433-111452 None:intergenic 35.0%
! GATAGTGAAGATGATGATGA+TGG + tig0021636:111473-111492 Msa1393280:CDS 35.0%
!! GTGAATTGAGGTTTGTTGTT+TGG - tig0021636:111665-111684 None:intergenic 35.0%
AACAATGAAGCAGTGAATTG+AGG - tig0021636:111677-111696 None:intergenic 35.0%
!! TCAAGAACAGAATCGGTTTT+TGG - tig0021636:111746-111765 None:intergenic 35.0%
CTGGTGAAAAGTGAAACAAT+GGG - tig0021636:111818-111837 None:intergenic 35.0%
TCTGGTGAAAAGTGAAACAA+TGG - tig0021636:111819-111838 None:intergenic 35.0%
CATGTTCAACTTTCTGATTC+TGG - tig0021636:111883-111902 None:intergenic 35.0%
TGAACCATCATTATCACCTT+TGG + tig0021636:111901-111920 Msa1393280:CDS 35.0%
! TCTTCCAAAGGTGATAATGA+TGG - tig0021636:111908-111927 None:intergenic 35.0%
GAAAAACGTTTCTCTTCCAA+AGG - tig0021636:111920-111939 None:intergenic 35.0%
!! GGAAGAGAAACGTTTTTCAA+AGG + tig0021636:111922-111941 Msa1393280:CDS 35.0%
TGGTGAAACCTCTTTACATA+AGG + tig0021636:111972-111991 Msa1393280:CDS 35.0%
GGTGAAACCTCTTTACATAA+GGG + tig0021636:111973-111992 Msa1393280:CDS 35.0%
TTTACATAAGGGTTTCTCGT+AGG + tig0021636:111984-112003 Msa1393280:CDS 35.0%
! GTTTTCTTCACTTTCAGCTA+CGG - tig0021636:112077-112096 None:intergenic 35.0%
GAAACAGGGGAATAATATTC+CGG + tig0021636:112117-112136 Msa1393280:CDS 35.0%
GTCCCTCAATTTAAATTGAC+GGG - tig0021636:112399-112418 None:intergenic 35.0%
CTTGATTTCCTTGACAATTC+TGG - tig0021636:112633-112652 None:intergenic 35.0%
! AGATTTTGCACTATGCATAG+TGG + tig0021636:112710-112729 Msa1393280:three_prime_UTR 35.0%
TAGTGGAGAGAAATAGTCAA+AGG + tig0021636:112727-112746 Msa1393280:three_prime_UTR 35.0%
ATAGTCAAAGGAGACAGTAA+TGG + tig0021636:112739-112758 Msa1393280:three_prime_UTR 35.0%
TCTTCTTTCCGATAAAGTGA+AGG + tig0021636:112873-112892 Msa1393280:three_prime_UTR 35.0%
GTGGTGAAGTGAGAAGAAAA+AGG - tig0021636:111121-111140 None:intergenic 40.0%
!! AGTGAAGATGATGATGATGG+TGG + tig0021636:111476-111495 Msa1393280:CDS 40.0%
TTGTCGAGAATGAAGGAAGA+AGG - tig0021636:111623-111642 None:intergenic 40.0%
! GTTGTTGTTGTCGAGAATGA+AGG - tig0021636:111630-111649 None:intergenic 40.0%
AGACATGAAGACACGAAACT+TGG - tig0021636:111711-111730 None:intergenic 40.0%
AAGTTTCGTGTCTTCATGTC+TGG + tig0021636:111710-111729 Msa1393280:CDS 40.0%
!!! GTTTTTGGAACTGTTGTCTC+TGG - tig0021636:111837-111856 None:intergenic 40.0%
CGAGAAACCCTTATGTAAAG+AGG - tig0021636:111983-112002 None:intergenic 40.0%
AAGAGTCAGAGTCAGAAACA+GGG + tig0021636:112103-112122 Msa1393280:CDS 40.0%
TCAAGTGACAAGAGACTTTC+AGG + tig0021636:112648-112667 Msa1393280:CDS 40.0%
!! GAGACTTTCAGGTTGAAGAA+AGG + tig0021636:112659-112678 Msa1393280:exon 40.0%
! TCAAAGGAGACAGTAATGGA+TGG + tig0021636:112743-112762 Msa1393280:three_prime_UTR 40.0%
!! CAAAGGAGACAGTAATGGAT+GGG + tig0021636:112744-112763 Msa1393280:three_prime_UTR 40.0%
GAGTAGAACTGTTGAGATGA+AGG + tig0021636:112781-112800 Msa1393280:three_prime_UTR 40.0%
GTGGTGAAGTGAGAAGAAAA+AGG - tig0021636:111121-111140 None:intergenic 40.0%
!! AGTGAAGATGATGATGATGG+TGG + tig0021636:111476-111495 Msa1393280:CDS 40.0%
TTGTCGAGAATGAAGGAAGA+AGG - tig0021636:111623-111642 None:intergenic 40.0%
! GTTGTTGTTGTCGAGAATGA+AGG - tig0021636:111630-111649 None:intergenic 40.0%
AGACATGAAGACACGAAACT+TGG - tig0021636:111711-111730 None:intergenic 40.0%
AAGTTTCGTGTCTTCATGTC+TGG + tig0021636:111710-111729 Msa1393280:CDS 40.0%
!!! GTTTTTGGAACTGTTGTCTC+TGG - tig0021636:111837-111856 None:intergenic 40.0%
CGAGAAACCCTTATGTAAAG+AGG - tig0021636:111983-112002 None:intergenic 40.0%
AAGAGTCAGAGTCAGAAACA+GGG + tig0021636:112103-112122 Msa1393280:CDS 40.0%
TCAAGTGACAAGAGACTTTC+AGG + tig0021636:112648-112667 Msa1393280:CDS 40.0%
!! GAGACTTTCAGGTTGAAGAA+AGG + tig0021636:112659-112678 Msa1393280:exon 40.0%
! TCAAAGGAGACAGTAATGGA+TGG + tig0021636:112743-112762 Msa1393280:three_prime_UTR 40.0%
!! CAAAGGAGACAGTAATGGAT+GGG + tig0021636:112744-112763 Msa1393280:three_prime_UTR 40.0%
GAGTAGAACTGTTGAGATGA+AGG + tig0021636:112781-112800 Msa1393280:three_prime_UTR 40.0%
ACAGTGAATATGTTGGGCAG+TGG - tig0021636:111140-111159 None:intergenic 45.0%
!! TATACTGGCTCTCCCAACAA+TGG + tig0021636:111181-111200 Msa1393280:exon 45.0%
! AGCTTGCTTAAATACTGGCG+AGG + tig0021636:111212-111231 Msa1393280:CDS 45.0%
TTGCTTAAATACTGGCGAGG+TGG + tig0021636:111215-111234 Msa1393280:CDS 45.0%
!! GAGAATGGTGGTGGTATTGA+CGG - tig0021636:111291-111310 None:intergenic 45.0%
! AGCGACGACAATGATGATGA+AGG + tig0021636:111326-111345 Msa1393280:CDS 45.0%
TCTTTCACTGTCACCTTCAG+AGG + tig0021636:111562-111581 Msa1393280:CDS 45.0%
CCATGGTGATAAATCAAGCC+CGG + tig0021636:112012-112031 Msa1393280:CDS 45.0%
CATGGTGATAAATCAAGCCC+GGG + tig0021636:112013-112032 Msa1393280:CDS 45.0%
TAAATCAAGCCCGGGTGAAA+AGG + tig0021636:112021-112040 Msa1393280:CDS 45.0%
! TCTGCTTCAACCTTTTCACC+CGG - tig0021636:112034-112053 None:intergenic 45.0%
GGTTGAAGCAGAAATAGCGA+CGG + tig0021636:112042-112061 Msa1393280:CDS 45.0%
!! CTGTTTCTGACTCTGACTCT+TGG - tig0021636:112104-112123 None:intergenic 45.0%
CAAGAGTCAGAGTCAGAAAC+AGG + tig0021636:112102-112121 Msa1393280:CDS 45.0%
AGAGTCAGAGTCAGAAACAG+GGG + tig0021636:112104-112123 Msa1393280:CDS 45.0%
AGGGGAATAATATTCCGGCA+GGG + tig0021636:112122-112141 Msa1393280:CDS 45.0%
TAAACAACTCTTAGCCCTGC+CGG - tig0021636:112139-112158 None:intergenic 45.0%
GCTGCAAAAGCGAATCATCA+CGG - tig0021636:112237-112256 None:intergenic 45.0%
CAGCAGCAAGATGGAATTCA+AGG + tig0021636:112253-112272 Msa1393280:CDS 45.0%
AGATCCTTCAATGCATCCAG+AGG + tig0021636:112295-112314 Msa1393280:CDS 45.0%
TTCACCTCTGGATGCATTGA+AGG - tig0021636:112302-112321 None:intergenic 45.0%
AATGGATCACTCACTGAACG+TGG - tig0021636:112597-112616 None:intergenic 45.0%
GTTCAGTGAGTGATCCATTG+CGG + tig0021636:112598-112617 Msa1393280:CDS 45.0%
TTCAGTGAGTGATCCATTGC+GGG + tig0021636:112599-112618 Msa1393280:CDS 45.0%
TCTGGTGAAATTTCCCGCAA+TGG - tig0021636:112615-112634 None:intergenic 45.0%
ACAGTGAATATGTTGGGCAG+TGG - tig0021636:111140-111159 None:intergenic 45.0%
!! TATACTGGCTCTCCCAACAA+TGG + tig0021636:111181-111200 Msa1393280:exon 45.0%
! AGCTTGCTTAAATACTGGCG+AGG + tig0021636:111212-111231 Msa1393280:CDS 45.0%
TTGCTTAAATACTGGCGAGG+TGG + tig0021636:111215-111234 Msa1393280:CDS 45.0%
!! GAGAATGGTGGTGGTATTGA+CGG - tig0021636:111291-111310 None:intergenic 45.0%
! AGCGACGACAATGATGATGA+AGG + tig0021636:111326-111345 Msa1393280:CDS 45.0%
TCTTTCACTGTCACCTTCAG+AGG + tig0021636:111562-111581 Msa1393280:CDS 45.0%
CCATGGTGATAAATCAAGCC+CGG + tig0021636:112012-112031 Msa1393280:CDS 45.0%
CATGGTGATAAATCAAGCCC+GGG + tig0021636:112013-112032 Msa1393280:CDS 45.0%
TAAATCAAGCCCGGGTGAAA+AGG + tig0021636:112021-112040 Msa1393280:CDS 45.0%
! TCTGCTTCAACCTTTTCACC+CGG - tig0021636:112034-112053 None:intergenic 45.0%
GGTTGAAGCAGAAATAGCGA+CGG + tig0021636:112042-112061 Msa1393280:CDS 45.0%
!! CTGTTTCTGACTCTGACTCT+TGG - tig0021636:112104-112123 None:intergenic 45.0%
CAAGAGTCAGAGTCAGAAAC+AGG + tig0021636:112102-112121 Msa1393280:CDS 45.0%
AGAGTCAGAGTCAGAAACAG+GGG + tig0021636:112104-112123 Msa1393280:CDS 45.0%
AGGGGAATAATATTCCGGCA+GGG + tig0021636:112122-112141 Msa1393280:CDS 45.0%
TAAACAACTCTTAGCCCTGC+CGG - tig0021636:112139-112158 None:intergenic 45.0%
GCTGCAAAAGCGAATCATCA+CGG - tig0021636:112237-112256 None:intergenic 45.0%
CAGCAGCAAGATGGAATTCA+AGG + tig0021636:112253-112272 Msa1393280:CDS 45.0%
AGATCCTTCAATGCATCCAG+AGG + tig0021636:112295-112314 Msa1393280:CDS 45.0%
TTCACCTCTGGATGCATTGA+AGG - tig0021636:112302-112321 None:intergenic 45.0%
AATGGATCACTCACTGAACG+TGG - tig0021636:112597-112616 None:intergenic 45.0%
GTTCAGTGAGTGATCCATTG+CGG + tig0021636:112598-112617 Msa1393280:CDS 45.0%
TTCAGTGAGTGATCCATTGC+GGG + tig0021636:112599-112618 Msa1393280:CDS 45.0%
TCTGGTGAAATTTCCCGCAA+TGG - tig0021636:112615-112634 None:intergenic 45.0%
CTTAAATACTGGCGAGGTGG+TGG + tig0021636:111218-111237 Msa1393280:CDS 50.0%
!! AATGGTGGTGGTATTGACGG+TGG - tig0021636:111288-111307 None:intergenic 50.0%
! ATTGTCGTCGCTGCTTTCAG+CGG - tig0021636:111318-111337 None:intergenic 50.0%
GTTTCTCGTAGGTACAGCCA+TGG + tig0021636:111995-112014 Msa1393280:CDS 50.0%
CCGGGCTTGATTTATCACCA+TGG - tig0021636:112015-112034 None:intergenic 50.0%
! CTGCTTCAACCTTTTCACCC+GGG - tig0021636:112033-112052 None:intergenic 50.0%
GAAGCAGAAATAGCGACGGA+AGG + tig0021636:112046-112065 Msa1393280:CDS 50.0%
CAGGGGAATAATATTCCGGC+AGG + tig0021636:112121-112140 Msa1393280:CDS 50.0%
AGCGAATCATCACGGCGTTT+CGG - tig0021636:112229-112248 None:intergenic 50.0%
!! TCGCTTTTGCAGCAGCAAGA+TGG + tig0021636:112244-112263 Msa1393280:CDS 50.0%
CTTAAATACTGGCGAGGTGG+TGG + tig0021636:111218-111237 Msa1393280:CDS 50.0%
!! AATGGTGGTGGTATTGACGG+TGG - tig0021636:111288-111307 None:intergenic 50.0%
! ATTGTCGTCGCTGCTTTCAG+CGG - tig0021636:111318-111337 None:intergenic 50.0%
GTTTCTCGTAGGTACAGCCA+TGG + tig0021636:111995-112014 Msa1393280:CDS 50.0%
CCGGGCTTGATTTATCACCA+TGG - tig0021636:112015-112034 None:intergenic 50.0%
! CTGCTTCAACCTTTTCACCC+GGG - tig0021636:112033-112052 None:intergenic 50.0%
GAAGCAGAAATAGCGACGGA+AGG + tig0021636:112046-112065 Msa1393280:CDS 50.0%
CAGGGGAATAATATTCCGGC+AGG + tig0021636:112121-112140 Msa1393280:CDS 50.0%
AGCGAATCATCACGGCGTTT+CGG - tig0021636:112229-112248 None:intergenic 50.0%
!! TCGCTTTTGCAGCAGCAAGA+TGG + tig0021636:112244-112263 Msa1393280:CDS 50.0%
!! GTGGTGGTGGAGTCAGAAGA+AGG - tig0021636:111263-111282 None:intergenic 55.0%
TTCAGCGGTGGTGAGAATGG+TGG - tig0021636:111303-111322 None:intergenic 55.0%
GCTTTCAGCGGTGGTGAGAA+TGG - tig0021636:111306-111325 None:intergenic 55.0%
CGTTTCGGCGACACTAACAC+CGG - tig0021636:112214-112233 None:intergenic 55.0%
!! GTGGTGGTGGAGTCAGAAGA+AGG - tig0021636:111263-111282 None:intergenic 55.0%
TTCAGCGGTGGTGAGAATGG+TGG - tig0021636:111303-111322 None:intergenic 55.0%
GCTTTCAGCGGTGGTGAGAA+TGG - tig0021636:111306-111325 None:intergenic 55.0%
CGTTTCGGCGACACTAACAC+CGG - tig0021636:112214-112233 None:intergenic 55.0%
!! CGAGGTGGTGGTGCTGTAGT+TGG + tig0021636:111230-111249 Msa1393280:CDS 60.0%
!! ATTGACGGTGGTGGTGGTGG+TGG - tig0021636:111276-111295 None:intergenic 60.0%
!! GGTATTGACGGTGGTGGTGG+TGG - tig0021636:111279-111298 None:intergenic 60.0%
!! GGTGGTATTGACGGTGGTGG+TGG - tig0021636:111282-111301 None:intergenic 60.0%
!! GGTGGTGGTATTGACGGTGG+TGG - tig0021636:111285-111304 None:intergenic 60.0%
AGCGGTGGTGAGAATGGTGG+TGG - tig0021636:111300-111319 None:intergenic 60.0%
GAAGAGCCGTTCCGCGTCTT+CGG + tig0021636:112171-112190 Msa1393280:CDS 60.0%
TTCGGCGACACTAACACCGG+CGG - tig0021636:112211-112230 None:intergenic 60.0%
!! CGAGGTGGTGGTGCTGTAGT+TGG + tig0021636:111230-111249 Msa1393280:CDS 60.0%
!! ATTGACGGTGGTGGTGGTGG+TGG - tig0021636:111276-111295 None:intergenic 60.0%
!! GGTATTGACGGTGGTGGTGG+TGG - tig0021636:111279-111298 None:intergenic 60.0%
!! GGTGGTATTGACGGTGGTGG+TGG - tig0021636:111282-111301 None:intergenic 60.0%
!! GGTGGTGGTATTGACGGTGG+TGG - tig0021636:111285-111304 None:intergenic 60.0%
AGCGGTGGTGAGAATGGTGG+TGG - tig0021636:111300-111319 None:intergenic 60.0%
GAAGAGCCGTTCCGCGTCTT+CGG + tig0021636:112171-112190 Msa1393280:CDS 60.0%
TTCGGCGACACTAACACCGG+CGG - tig0021636:112211-112230 None:intergenic 60.0%
! GTCGTCGCTGCTTTCAGCGG+TGG - tig0021636:111315-111334 None:intergenic 65.0%
CCACTGCCGAAGACGCGGAA+CGG - tig0021636:112180-112199 None:intergenic 65.0%
TTCCGCGTCTTCGGCAGTGG+CGG + tig0021636:112180-112199 Msa1393280:CDS 65.0%
! GTCGTCGCTGCTTTCAGCGG+TGG - tig0021636:111315-111334 None:intergenic 65.0%
CCACTGCCGAAGACGCGGAA+CGG - tig0021636:112180-112199 None:intergenic 65.0%
TTCCGCGTCTTCGGCAGTGG+CGG + tig0021636:112180-112199 Msa1393280:CDS 65.0%
CCGTTCCGCGTCTTCGGCAG+TGG + tig0021636:112177-112196 Msa1393280:CDS 70.0%
AGCCGCCACTGCCGAAGACG+CGG - tig0021636:112185-112204 None:intergenic 70.0%
CCGTTCCGCGTCTTCGGCAG+TGG + tig0021636:112177-112196 Msa1393280:CDS 70.0%
AGCCGCCACTGCCGAAGACG+CGG - tig0021636:112185-112204 None:intergenic 70.0%
GGCAGTGGCGGCTTCTCCGC+CGG + tig0021636:112192-112211 Msa1393280:CDS 75.0%
GGCAGTGGCGGCTTCTCCGC+CGG + tig0021636:112192-112211 Msa1393280:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0021636 gene 111054 112911 111054 ID=Msa1393280;Name=Msa1393280
tig0021636 mRNA 111054 112911 111054 ID=Msa1393280-mRNA-1;Parent=Msa1393280;Name=Msa1393280-mRNA-1;_AED=0.00;_eAED=0.00;_QI=146|1|1|1|1|1|2|237|406
tig0021636 exon 111054 112316 111054 ID=Msa1393280-mRNA-1:exon:13610;Parent=Msa1393280-mRNA-1
tig0021636 exon 112571 112911 112571 ID=Msa1393280-mRNA-1:exon:13611;Parent=Msa1393280-mRNA-1
tig0021636 five_prime_UTR 111054 111199 111054 ID=Msa1393280-mRNA-1:five_prime_utr;Parent=Msa1393280-mRNA-1
tig0021636 CDS 111200 112316 111200 ID=Msa1393280-mRNA-1:cds;Parent=Msa1393280-mRNA-1
tig0021636 CDS 112571 112674 112571 ID=Msa1393280-mRNA-1:cds;Parent=Msa1393280-mRNA-1
tig0021636 three_prime_UTR 112675 112911 112675 ID=Msa1393280-mRNA-1:three_prime_utr;Parent=Msa1393280-mRNA-1
Gene Sequence

>Msa1393280

ATGGAAGCTTTTAGCTTGCTTAAATACTGGCGAGGTGGTGGTGCTGTAGTTGGTCTCTCTCCTTCTTCTGACTCCACCACCACCACCACCGTCAATACCACCACCATTCTCACCACCGCTGAAAGCAGCGACGACAATGATGATGAAGGTCCTTTCTTTGACTTAGAGTTTACTGCACCTGAAGAAGAAGAAGAAGAAGAAGATGGGTCTGAAGAAACCAACCATAACAACCAACAACAACAACAACAAGAACTAGATGAAGAAGATTCTGATGATAGTGAAGATGATGATGATGGTGGTGAGACTGAGAGTGAGTTTAAGATAACACTGTCACCTTCAAACAATGAAAGAAGTGATGCAAATCTTTCACTGTCACCTTCAGAGGATTTATTCTTCAAAGGAAAGCTTGTTCAGCTGAAACCTTCTTCCTTCATTCTCGACAACAACAACAACAGTTCAGAACCAAACAACAAACCTCAATTCACTGCTTCATTGTTAAAATCAGCAACCAAGTTTCGTGTCTTCATGTCTGGTCTCAAGAAACCAAAAACCGATTCTGTTCTTGAAAAGAAGCAACACAAACCAGAGAGAATCAAATTCAAAGTAGAAGAAGTTCCCATTGTTTCACTTTTCACCAGAGACAACAGTTCCAAAAACAGCAACCACAAATATCAAACAAACCAGAATCAGAAAGTTGAACATGAACCATCATTATCACCTTTGGAAGAGAAACGTTTTTCAAAGGAAGTAGTTATGCACAAGTATTTGAAAATGGTGAAACCTCTTTACATAAGGGTTTCTCGTAGGTACAGCCATGGTGATAAATCAAGCCCGGGTGAAAAGGTTGAAGCAGAAATAGCGACGGAAGGTGAAACCGTAGCTGAAAGTGAAGAAAACAATGCCAAGAGTCAGAGTCAGAAACAGGGGAATAATATTCCGGCAGGGCTAAGAGTTGTTTACAAACATTTAGGGAAGAGCCGTTCCGCGTCTTCGGCAGTGGCGGCTTCTCCGCCGGTGTTAGTGTCGCCGAAACGCCGTGATGATTCGCTTTTGCAGCAGCAAGATGGAATTCAAGGAGCTATTTTACACTGCAAGAGATCCTTCAATGCATCCAGAGAGTGTGACTCTTCTTCTCAACTACCACGTTCAGTGAGTGATCCATTGCGGGAAATTTCACCAGAATTGTCAAGGAAATCAAGTGACAAGAGACTTTCAGGTTGA
Protein sequence

>Msa1393280

MEAFSLLKYWRGGGAVVGLSPSSDSTTTTTVNTTTILTTAESSDDNDDEGPFFDLEFTAPEEEEEEEDGSEETNHNNQQQQQQELDEEDSDDSEDDDDGGETESEFKITLSPSNNERSDANLSLSPSEDLFFKGKLVQLKPSSFILDNNNNSSEPNNKPQFTASLLKSATKFRVFMSGLKKPKTDSVLEKKQHKPERIKFKVEEVPIVSLFTRDNSSKNSNHKYQTNQNQKVEHEPSLSPLEEKRFSKEVVMHKYLKMVKPLYIRVSRRYSHGDKSSPGEKVEAEIATEGETVAESEENNAKSQSQKQGNNIPAGLRVVYKHLGKSRSASSAVAASPPVLVSPKRRDDSLLQQQDGIQGAILHCKRSFNASRECDSSSQLPRSVSDPLREISPELSRKSSDKRLSG*