AlfalfaGEDB | Msa1465310

M. sativa cultivar zhongmu-4 / Msa1465310

KEGG: K01191
GO: GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005774 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006517 GO:0006807 GO:0008150 GO:0008152 GO:0009100 GO:0009505 GO:0009987 GO:0015923 GO:0016020 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0030312 GO:0031090 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071704 GO:0071944 GO:0098588 GO:0098805 GO:1901135 GO:1901564
PFAM: PF17677.4

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1465310	XP_003624502.1	95.614	114	4	1	1	114	910	1022	4.77e-63	217

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1465310	sp\|P94078\|MANA1_ARATH	70.000	100	28	1	1	100	907	1004	6.27e-37	135

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1465310	G7KX96	95.614	114	4	1	1	114	910	1022	2.28e-63	217

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003700	Msa1465310	0.819172	1.335453e-52	-8.615850e-47
Msa0020930	Msa1465310	0.800435	1.443477e-48	-8.615850e-47
Msa0094120	Msa1465310	0.800976	1.119414e-48	-8.615850e-47
Msa0110970	Msa1465310	0.816144	6.434229e-52	-8.615850e-47
Msa1374040	Msa1465310	0.813868	2.058222e-51	-8.615850e-47
Msa1375560	Msa1465310	0.818174	2.249101e-52	-8.615850e-47
Msa1382540	Msa1465310	0.814187	1.750100e-51	-8.615850e-47
Msa1417420	Msa1465310	0.809371	1.955641e-50	-8.615850e-47
Msa1418090	Msa1465310	0.805338	1.400682e-49	-8.615850e-47
Msa1464810	Msa1465310	0.819606	1.063126e-52	-8.615850e-47
Msa0669320	Msa1465310	0.808059	3.729887e-50	-8.615850e-47
Msa0796530	Msa1465310	0.808080	3.692441e-50	-8.615850e-47
Msa0146260	Msa1465310	0.816221	6.184422e-52	-8.615850e-47
Msa0200260	Msa1465310	0.802017	6.848566e-49	-8.615850e-47
Msa0225740	Msa1465310	0.805920	1.057425e-49	-8.615850e-47
Msa1233910	Msa1465310	0.802767	4.798183e-49	-8.615850e-47
Msa0376350	Msa1465310	0.811346	7.330201e-51	-8.615850e-47
Msa0410650	Msa1465310	0.806706	7.222977e-50	-8.615850e-47
Msa0424790	Msa1465310	0.813321	2.715773e-51	-8.615850e-47
Msa0428150	Msa1465310	0.808470	3.048656e-50	-8.615850e-47
Msa0462500	Msa1465310	0.803541	3.318534e-49	-8.615850e-47
Msa0479170	Msa1465310	0.814681	1.361089e-51	-8.615850e-47
Msa1023400	Msa1465310	0.811474	6.875818e-51	-8.615850e-47
Msa1085200	Msa1465310	0.805125	1.552464e-49	-8.615850e-47
Msa0243100	Msa1465310	0.805679	1.188236e-49	-8.615850e-47
Msa0340090	Msa1465310	0.800564	1.358634e-48	-8.615850e-47
Msa1111130	Msa1465310	0.806290	8.840454e-50	-8.615850e-47
Msa1209460	Msa1465310	0.809046	2.295641e-50	-8.615850e-47
Msa0507680	Msa1465310	0.823703	1.200577e-53	-8.615850e-47
Msa0509710	Msa1465310	0.808837	2.544613e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1465310	MtrunA17_Chr7g0252171	95.614	114	4	1	1	114	910	1022	4.39e-67	217
Msa1465310	MtrunA17_Chr3g0131651	68.687	99	30	1	1	99	917	1014	9.29e-41	143
Msa1465310	MtrunA17_Chr7g0252151	70.000	100	29	1	1	100	954	1052	2.12e-39	139
Msa1465310	MtrunA17_Chr8g0371581	58.586	99	39	1	1	99	922	1018	1.32e-31	117

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1465310	AT3G26720.1	70.000	100	28	1	1	100	907	1004	6.38e-38	135
Msa1465310	AT5G13980.1	69.697	99	28	1	1	97	910	1008	4.80e-37	132
Msa1465310	AT5G13980.2	69.697	99	28	1	1	97	910	1008	4.80e-37	132
Msa1465310	AT5G66150.3	63.636	99	35	1	1	99	644	741	8.69e-28	106
Msa1465310	AT5G66150.1	63.636	99	35	1	1	99	938	1035	9.81e-28	106
Msa1465310	AT5G66150.2	63.636	99	35	1	1	99	937	1034	1.09e-27	106
Msa1465310	AT3G26720.5	71.053	38	11	0	1	38	907	944	5.35e-11	58.5

Find 44 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATTTATTTACAAGCAAATT+TGG	0.146106	tig0045145:-1019	Msa1465310:intergenic
GGATTGGGTTGGTTTGGTTT+TGG	0.286406	tig0045145:-1212	Msa1465310:intergenic
AGATTAGTTTGGAAGGTAGA+AGG	0.292289	tig0045145:+778	Msa1465310:CDS
TGGAAAAGAAGAGATTAGTT+TGG	0.318017	tig0045145:+767	Msa1465310:CDS
AGTTCAACAACCAGCTTTGT+AGG	0.342923	tig0045145:-847	Msa1465310:intergenic
CAACGATTCGATGAGATCAT+TGG	0.353498	tig0045145:-1161	Msa1465310:intergenic
AGGCTCCAGTGAAGAGTCTA+AGG	0.354287	tig0045145:+801	Msa1465310:CDS
AAACATGGATTGGGTTGGTT+TGG	0.368850	tig0045145:-1218	Msa1465310:intergenic
TAATCAAGAAAGAGCTGAAA+TGG	0.371503	tig0045145:+747	Msa1465310:CDS
CTGCTGCAGGAACTGTTTGA+AGG	0.383150	tig0045145:-912	Msa1465310:intergenic
AATGGAGATTCGGACATTCT+TGG	0.400784	tig0045145:+876	Msa1465310:CDS
AACTTGTTCCAATGGAGATT+CGG	0.406586	tig0045145:+866	Msa1465310:CDS
ACCACTCTTACCTTCTTGTT+AGG	0.407090	tig0045145:-623	Msa1465310:intergenic
GAGAAGATACAAACATGGAT+TGG	0.414450	tig0045145:-1228	Msa1465310:intergenic
ACCAGCTTTGTAGGATCAAC+TGG	0.421591	tig0045145:-838	Msa1465310:intergenic
GATGCATGGGAGAGCTGTAT+AGG	0.422087	tig0045145:+1297	Msa1465310:three_prime_UTR
AGAAGATACAAACATGGATT+GGG	0.483372	tig0045145:-1227	Msa1465310:intergenic
TGCTGCAGGAACTGTTTGAA+GGG	0.496123	tig0045145:-911	Msa1465310:intergenic
AAACGTACATTCTTCTCTGT+TGG	0.497513	tig0045145:-963	Msa1465310:intergenic
AAAGAAGAGATTAGTTTGGA+AGG	0.497971	tig0045145:+771	Msa1465310:CDS
GCATCATTGCATAGGTGCAT+TGG	0.507082	tig0045145:-1279	Msa1465310:intergenic
AAGAATGTCCGAATCTCCAT+TGG	0.513779	tig0045145:-874	Msa1465310:intergenic
TGAAGAGTCTAAGGTGGTGA+GGG	0.513966	tig0045145:+810	Msa1465310:CDS
ATGAGATCATTGGATTGACC+AGG	0.526885	tig0045145:-1151	Msa1465310:intergenic
ACCAGTTGATCCTACAAAGC+TGG	0.532090	tig0045145:+837	Msa1465310:CDS
GATACAAACATGGATTGGGT+TGG	0.540361	tig0045145:-1223	Msa1465310:intergenic
GGTTGTTGAACTTGTTCCAA+TGG	0.548030	tig0045145:+858	Msa1465310:CDS
CACCACCTTAGACTCTTCAC+TGG	0.548230	tig0045145:-806	Msa1465310:intergenic
GACCAGGGTGCCTGTACTCA+CGG	0.549204	tig0045145:-1135	Msa1465310:intergenic
TGCACCTATGCAATGATGCA+TGG	0.587264	tig0045145:+1283	Msa1465310:three_prime_UTR
GCTGCAGGAACTGTTTGAAG+GGG	0.591936	tig0045145:-910	Msa1465310:intergenic
GTCCGTGAGTACAGGCACCC+TGG	0.592819	tig0045145:+1133	Msa1465310:three_prime_UTR
TCTCCCATGCATCATTGCAT+AGG	0.594279	tig0045145:-1287	Msa1465310:intergenic
CATTGCATAGGTGCATTGGA+AGG	0.600006	tig0045145:-1275	Msa1465310:intergenic
GTGAAGAGTCTAAGGTGGTG+AGG	0.606014	tig0045145:+809	Msa1465310:CDS
GCACCTATGCAATGATGCAT+GGG	0.614893	tig0045145:+1284	Msa1465310:three_prime_UTR
TTAGTTTGGAAGGTAGAAGG+AGG	0.619123	tig0045145:+781	Msa1465310:CDS
GAGTCTAAGGTGGTGAGGGG+AGG	0.623763	tig0045145:+814	Msa1465310:CDS
TGCACAATGAGACTTTCTAG+AGG	0.650534	tig0045145:+1088	Msa1465310:three_prime_UTR
GAAGAGTCTAAGGTGGTGAG+GGG	0.652985	tig0045145:+811	Msa1465310:CDS
ACTACATGTAAAGGTTGGAG+AGG	0.656333	tig0045145:+553	Msa1465310:intron
CTCCAGTGAAGAGTCTAAGG+TGG	0.687062	tig0045145:+804	Msa1465310:CDS
TGAGATCATTGGATTGACCA+GGG	0.690082	tig0045145:-1150	Msa1465300:intergenic
AAAATGAGAAGATACAAACA+TGG	0.708935	tig0045145:-1233	Msa1465300:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAATTTCCTTAATTTGAAAA+AGG	-	tig0045145:155-174	Msa1465310:intergenic	15.0%
!!	TATTTATTTACAAGCAAATT+TGG	-	tig0045145:1022-1041	Msa1465310:intergenic	15.0%
!!!	TAATTTCCTTAATTTGAAAA+AGG	-	tig0045145:155-174	Msa1465310:intergenic	15.0%
!!	TATTTATTTACAAGCAAATT+TGG	-	tig0045145:1022-1041	Msa1465310:intergenic	15.0%
!!	AAATAATTACAACCTCATAA+AGG	-	tig0045145:30-49	Msa1465310:intergenic	20.0%
!!	GATTTAGTGATAATAATCTT+TGG	+	tig0045145:54-73	Msa1465310:intron	20.0%
!!!	TACTTTCCTTTTTCAAATTA+AGG	+	tig0045145:146-165	Msa1465310:intron	20.0%
!!!	ATCATCAATGTCTTTTATTA+AGG	+	tig0045145:1357-1376	Msa1465310:three_prime_UTR	20.0%
!!	AAATAATTACAACCTCATAA+AGG	-	tig0045145:30-49	Msa1465310:intergenic	20.0%
!!	GATTTAGTGATAATAATCTT+TGG	+	tig0045145:54-73	Msa1465310:intron	20.0%
!!!	TACTTTCCTTTTTCAAATTA+AGG	+	tig0045145:146-165	Msa1465310:intron	20.0%
!!!	AATAATCTTTGGTTCTTTCT+TGG	+	tig0045145:65-84	Msa1465310:intron	25.0%
!!	TAAGTCTATATTTTCCTTTG+CGG	-	tig0045145:336-355	Msa1465310:intergenic	25.0%
!	TTGCATTCTTAACTATGATT+GGG	+	tig0045145:450-469	Msa1465310:intron	25.0%
!	AATTTCTTTGCAAGTAAAAC+AGG	-	tig0045145:492-511	Msa1465310:intergenic	25.0%
!!!	CTTTTTCAATTCTACATTTG+CGG	-	tig0045145:597-616	Msa1465310:intergenic	25.0%
!!	GCAAAAAATAGTTTGGAATA+TGG	-	tig0045145:655-674	Msa1465310:intergenic	25.0%
!	AAAAAGTTAGAGTTGTTAGT+AGG	-	tig0045145:692-711	Msa1465310:intergenic	25.0%
!	AAAATGAGAAGATACAAACA+TGG	-	tig0045145:1236-1255	Msa1465310:intergenic	25.0%
!!!	AATAATCTTTGGTTCTTTCT+TGG	+	tig0045145:65-84	Msa1465310:intron	25.0%
!!	TAAGTCTATATTTTCCTTTG+CGG	-	tig0045145:336-355	Msa1465310:intergenic	25.0%
!	TTGCATTCTTAACTATGATT+GGG	+	tig0045145:450-469	Msa1465310:intron	25.0%
!	AATTTCTTTGCAAGTAAAAC+AGG	-	tig0045145:492-511	Msa1465310:intergenic	25.0%
!!!	CTTTTTCAATTCTACATTTG+CGG	-	tig0045145:597-616	Msa1465310:intergenic	25.0%
!!	GCAAAAAATAGTTTGGAATA+TGG	-	tig0045145:655-674	Msa1465310:intergenic	25.0%
!	AAAAAGTTAGAGTTGTTAGT+AGG	-	tig0045145:692-711	Msa1465310:intergenic	25.0%
!	AAAATGAGAAGATACAAACA+TGG	-	tig0045145:1236-1255	Msa1465310:intergenic	25.0%
	CTTGCATTCTTAACTATGAT+TGG	+	tig0045145:449-468	Msa1465310:intron	30.0%
	ATTCTTAACTATGATTGGGA+TGG	+	tig0045145:454-473	Msa1465310:intron	30.0%
	TCGTGTATTACTACATGTAA+AGG	+	tig0045145:544-563	Msa1465310:intron	30.0%
	GTATTACTACATGTAAAGGT+TGG	+	tig0045145:548-567	Msa1465310:intron	30.0%
	ACATGCAGCAAAAAATAGTT+TGG	-	tig0045145:662-681	Msa1465310:intergenic	30.0%
	TAATCAAGAAAGAGCTGAAA+TGG	+	tig0045145:747-766	Msa1465310:CDS	30.0%
!	TGGAAAAGAAGAGATTAGTT+TGG	+	tig0045145:767-786	Msa1465310:CDS	30.0%
!!	AAAGAAGAGATTAGTTTGGA+AGG	+	tig0045145:771-790	Msa1465310:CDS	30.0%
!!!	AAGCTTTTTAACTTGAGTGA+TGG	+	tig0045145:1044-1063	Msa1465310:three_prime_UTR	30.0%
!!	AGAGGTTTTAATCTTGAAAG+TGG	+	tig0045145:1106-1125	Msa1465310:three_prime_UTR	30.0%
!!	GAGGTTTTAATCTTGAAAGT+GGG	+	tig0045145:1107-1126	Msa1465310:three_prime_UTR	30.0%
!!	AGGTTTTAATCTTGAAAGTG+GGG	+	tig0045145:1108-1127	Msa1465310:three_prime_UTR	30.0%
	AGAAGATACAAACATGGATT+GGG	-	tig0045145:1230-1249	Msa1465310:intergenic	30.0%
	CTTGCATTCTTAACTATGAT+TGG	+	tig0045145:449-468	Msa1465310:intron	30.0%
	ATTCTTAACTATGATTGGGA+TGG	+	tig0045145:454-473	Msa1465310:intron	30.0%
	TCGTGTATTACTACATGTAA+AGG	+	tig0045145:544-563	Msa1465310:intron	30.0%
	GTATTACTACATGTAAAGGT+TGG	+	tig0045145:548-567	Msa1465310:intron	30.0%
	ACATGCAGCAAAAAATAGTT+TGG	-	tig0045145:662-681	Msa1465310:intergenic	30.0%
	TAATCAAGAAAGAGCTGAAA+TGG	+	tig0045145:747-766	Msa1465310:CDS	30.0%
!	TGGAAAAGAAGAGATTAGTT+TGG	+	tig0045145:767-786	Msa1465310:CDS	30.0%
!!	AAAGAAGAGATTAGTTTGGA+AGG	+	tig0045145:771-790	Msa1465310:CDS	30.0%
!!!	AAGCTTTTTAACTTGAGTGA+TGG	+	tig0045145:1044-1063	Msa1465310:three_prime_UTR	30.0%
!!	AGAGGTTTTAATCTTGAAAG+TGG	+	tig0045145:1106-1125	Msa1465310:three_prime_UTR	30.0%
!!	GAGGTTTTAATCTTGAAAGT+GGG	+	tig0045145:1107-1126	Msa1465310:three_prime_UTR	30.0%
!!	AGGTTTTAATCTTGAAAGTG+GGG	+	tig0045145:1108-1127	Msa1465310:three_prime_UTR	30.0%
	AGAAGATACAAACATGGATT+GGG	-	tig0045145:1230-1249	Msa1465310:intergenic	30.0%
	CTTGGAGTAAAATTTCCCTT+AGG	+	tig0045145:83-102	Msa1465310:intron	35.0%
!!	CCTTTGCGGATTATTTTCTA+AGG	-	tig0045145:322-341	Msa1465310:intergenic	35.0%
	CCTTAGAAAATAATCCGCAA+AGG	+	tig0045145:319-338	Msa1465310:intron	35.0%
!!	CCTTTGCGGATTATTTTCTA+AGG	-	tig0045145:322-341	Msa1465310:intergenic	35.0%
	CCTTAGAAAATAATCCGCAA+AGG	+	tig0045145:319-338	Msa1465310:intron	35.0%
!	AAAGCTTTTGCCTAACAAGA+AGG	+	tig0045145:613-632	Msa1465310:CDS	35.0%
!!	AGATTAGTTTGGAAGGTAGA+AGG	+	tig0045145:778-797	Msa1465310:CDS	35.0%
!	AACTTGTTCCAATGGAGATT+CGG	+	tig0045145:866-885	Msa1465310:CDS	35.0%
	AAACGTACATTCTTCTCTGT+TGG	-	tig0045145:966-985	Msa1465310:intergenic	35.0%
!!	GGTTTTAATCTTGAAAGTGG+GGG	+	tig0045145:1109-1128	Msa1465310:three_prime_UTR	35.0%
	GAGAAGATACAAACATGGAT+TGG	-	tig0045145:1231-1250	Msa1465310:intergenic	35.0%
	CTTGGAGTAAAATTTCCCTT+AGG	+	tig0045145:83-102	Msa1465310:intron	35.0%
!!	CCTTTGCGGATTATTTTCTA+AGG	-	tig0045145:322-341	Msa1465310:intergenic	35.0%
	CCTTAGAAAATAATCCGCAA+AGG	+	tig0045145:319-338	Msa1465310:intron	35.0%
!!	CCTTTGCGGATTATTTTCTA+AGG	-	tig0045145:322-341	Msa1465310:intergenic	35.0%
	CCTTAGAAAATAATCCGCAA+AGG	+	tig0045145:319-338	Msa1465310:intron	35.0%
!	AAAGCTTTTGCCTAACAAGA+AGG	+	tig0045145:613-632	Msa1465310:CDS	35.0%
!!	AGATTAGTTTGGAAGGTAGA+AGG	+	tig0045145:778-797	Msa1465310:CDS	35.0%
!	AACTTGTTCCAATGGAGATT+CGG	+	tig0045145:866-885	Msa1465310:CDS	35.0%
	AAACGTACATTCTTCTCTGT+TGG	-	tig0045145:966-985	Msa1465310:intergenic	35.0%
!!	GGTTTTAATCTTGAAAGTGG+GGG	+	tig0045145:1109-1128	Msa1465310:three_prime_UTR	35.0%
	GAGAAGATACAAACATGGAT+TGG	-	tig0045145:1231-1250	Msa1465310:intergenic	35.0%
	CAAAATCCAACTCGCAAACA+AGG	-	tig0045145:109-128	Msa1465310:intergenic	40.0%
!!	CTTGTTTGCGAGTTGGATTT+TGG	+	tig0045145:107-126	Msa1465310:intron	40.0%
!!	TTTGCGAGTTGGATTTTGGA+GGG	+	tig0045145:111-130	Msa1465310:intron	40.0%
!!!	TTTGTGAGTTGGGTTTTGGA+GGG	-	tig0045145:294-313	Msa1465310:intergenic	40.0%
!!!	TTGGTTTGTGAGTTGGGTTT+TGG	-	tig0045145:298-317	Msa1465310:intergenic	40.0%
!!	TAAGGCTTGGTTTGTGAGTT+GGG	-	tig0045145:304-323	Msa1465310:intergenic	40.0%
!!!	GCGGATTATTTTCTAAGGCT+TGG	-	tig0045145:317-336	Msa1465310:intergenic	40.0%
!!!	TTTGTGAGTTGGGTTTTGGA+GGG	-	tig0045145:294-313	Msa1465310:intergenic	40.0%
!!!	TTGGTTTGTGAGTTGGGTTT+TGG	-	tig0045145:298-317	Msa1465310:intergenic	40.0%
!!	TAAGGCTTGGTTTGTGAGTT+GGG	-	tig0045145:304-323	Msa1465310:intergenic	40.0%
!!!	GCGGATTATTTTCTAAGGCT+TGG	-	tig0045145:317-336	Msa1465310:intergenic	40.0%
	GAAATCCTACTCAACCTCAA+CGG	-	tig0045145:426-445	Msa1465310:intergenic	40.0%
	ACTACATGTAAAGGTTGGAG+AGG	+	tig0045145:553-572	Msa1465310:intron	40.0%
	ACCACTCTTACCTTCTTGTT+AGG	-	tig0045145:626-645	Msa1465310:intergenic	40.0%
!	TTAGTTTGGAAGGTAGAAGG+AGG	+	tig0045145:781-800	Msa1465310:CDS	40.0%
!	AGTTCAACAACCAGCTTTGT+AGG	-	tig0045145:850-869	Msa1465310:intergenic	40.0%
!	GGTTGTTGAACTTGTTCCAA+TGG	+	tig0045145:858-877	Msa1465310:CDS	40.0%
	AAGAATGTCCGAATCTCCAT+TGG	-	tig0045145:877-896	Msa1465310:intergenic	40.0%
	AATGGAGATTCGGACATTCT+TGG	+	tig0045145:876-895	Msa1465310:CDS	40.0%
	TGCACAATGAGACTTTCTAG+AGG	+	tig0045145:1088-1107	Msa1465310:three_prime_UTR	40.0%
	TGAGATCATTGGATTGACCA+GGG	-	tig0045145:1153-1172	Msa1465310:intergenic	40.0%
	ATGAGATCATTGGATTGACC+AGG	-	tig0045145:1154-1173	Msa1465310:intergenic	40.0%
	CAACGATTCGATGAGATCAT+TGG	-	tig0045145:1164-1183	Msa1465310:intergenic	40.0%
!!	AAACATGGATTGGGTTGGTT+TGG	-	tig0045145:1221-1240	Msa1465310:intergenic	40.0%
!!	GATACAAACATGGATTGGGT+TGG	-	tig0045145:1226-1245	Msa1465310:intergenic	40.0%
	CAAAATCCAACTCGCAAACA+AGG	-	tig0045145:109-128	Msa1465310:intergenic	40.0%
!!	CTTGTTTGCGAGTTGGATTT+TGG	+	tig0045145:107-126	Msa1465310:intron	40.0%
!!	TTTGCGAGTTGGATTTTGGA+GGG	+	tig0045145:111-130	Msa1465310:intron	40.0%
!!!	TTTGTGAGTTGGGTTTTGGA+GGG	-	tig0045145:294-313	Msa1465310:intergenic	40.0%
!!!	TTGGTTTGTGAGTTGGGTTT+TGG	-	tig0045145:298-317	Msa1465310:intergenic	40.0%
!!	TAAGGCTTGGTTTGTGAGTT+GGG	-	tig0045145:304-323	Msa1465310:intergenic	40.0%
!!!	GCGGATTATTTTCTAAGGCT+TGG	-	tig0045145:317-336	Msa1465310:intergenic	40.0%
!!!	TTTGTGAGTTGGGTTTTGGA+GGG	-	tig0045145:294-313	Msa1465310:intergenic	40.0%
!!!	TTGGTTTGTGAGTTGGGTTT+TGG	-	tig0045145:298-317	Msa1465310:intergenic	40.0%
!!	TAAGGCTTGGTTTGTGAGTT+GGG	-	tig0045145:304-323	Msa1465310:intergenic	40.0%
!!!	GCGGATTATTTTCTAAGGCT+TGG	-	tig0045145:317-336	Msa1465310:intergenic	40.0%
	GAAATCCTACTCAACCTCAA+CGG	-	tig0045145:426-445	Msa1465310:intergenic	40.0%
	ACTACATGTAAAGGTTGGAG+AGG	+	tig0045145:553-572	Msa1465310:intron	40.0%
	ACCACTCTTACCTTCTTGTT+AGG	-	tig0045145:626-645	Msa1465310:intergenic	40.0%
!	TTAGTTTGGAAGGTAGAAGG+AGG	+	tig0045145:781-800	Msa1465310:CDS	40.0%
!	AGTTCAACAACCAGCTTTGT+AGG	-	tig0045145:850-869	Msa1465310:intergenic	40.0%
!	GGTTGTTGAACTTGTTCCAA+TGG	+	tig0045145:858-877	Msa1465310:CDS	40.0%
	AAGAATGTCCGAATCTCCAT+TGG	-	tig0045145:877-896	Msa1465310:intergenic	40.0%
	AATGGAGATTCGGACATTCT+TGG	+	tig0045145:876-895	Msa1465310:CDS	40.0%
	TGCACAATGAGACTTTCTAG+AGG	+	tig0045145:1088-1107	Msa1465310:three_prime_UTR	40.0%
	TGAGATCATTGGATTGACCA+GGG	-	tig0045145:1153-1172	Msa1465310:intergenic	40.0%
	ATGAGATCATTGGATTGACC+AGG	-	tig0045145:1154-1173	Msa1465310:intergenic	40.0%
	CAACGATTCGATGAGATCAT+TGG	-	tig0045145:1164-1183	Msa1465310:intergenic	40.0%
!!	AAACATGGATTGGGTTGGTT+TGG	-	tig0045145:1221-1240	Msa1465310:intergenic	40.0%
!!	GATACAAACATGGATTGGGT+TGG	-	tig0045145:1226-1245	Msa1465310:intergenic	40.0%
!	AAGACTGGCTCACCTTTATG+AGG	+	tig0045145:15-34	Msa1465310:CDS	45.0%
	AACTCGCAAACAAGGCCTAA+GGG	-	tig0045145:101-120	Msa1465310:intergenic	45.0%
!!	GTTTGCGAGTTGGATTTTGG+AGG	+	tig0045145:110-129	Msa1465310:intron	45.0%
!!	TTGCGAGTTGGATTTTGGAG+GGG	+	tig0045145:112-131	Msa1465310:intron	45.0%
!!	GTTTGTGAGTTGGGTTTTGG+AGG	-	tig0045145:295-314	Msa1465310:intergenic	45.0%
!!	CTAAGGCTTGGTTTGTGAGT+TGG	-	tig0045145:305-324	Msa1465310:intergenic	45.0%
!!	GTTTGTGAGTTGGGTTTTGG+AGG	-	tig0045145:295-314	Msa1465310:intergenic	45.0%
!!	CTAAGGCTTGGTTTGTGAGT+TGG	-	tig0045145:305-324	Msa1465310:intergenic	45.0%
	ATGTGCAATGCAAGCCTTTG+CGG	-	tig0045145:402-421	Msa1465310:intergenic	45.0%
	GCCTAACAAGAAGGTAAGAG+TGG	+	tig0045145:622-641	Msa1465310:intron	45.0%
!	TGAAGAGTCTAAGGTGGTGA+GGG	+	tig0045145:810-829	Msa1465310:CDS	45.0%
	ACCAGTTGATCCTACAAAGC+TGG	+	tig0045145:837-856	Msa1465310:CDS	45.0%
!	ACCAGCTTTGTAGGATCAAC+TGG	-	tig0045145:841-860	Msa1465310:intergenic	45.0%
	TGCTGCAGGAACTGTTTGAA+GGG	-	tig0045145:914-933	Msa1465310:intergenic	45.0%
!	GCTACATGTTTTTCTGCTGC+AGG	-	tig0045145:928-947	Msa1465310:intergenic	45.0%
!!!	GGATTGGGTTGGTTTGGTTT+TGG	-	tig0045145:1215-1234	Msa1465310:intergenic	45.0%
	CATTGCATAGGTGCATTGGA+AGG	-	tig0045145:1278-1297	Msa1465310:intergenic	45.0%
	GCATCATTGCATAGGTGCAT+TGG	-	tig0045145:1282-1301	Msa1465310:intergenic	45.0%
	TGCACCTATGCAATGATGCA+TGG	+	tig0045145:1283-1302	Msa1465310:three_prime_UTR	45.0%
	GCACCTATGCAATGATGCAT+GGG	+	tig0045145:1284-1303	Msa1465310:three_prime_UTR	45.0%
	TCTCCCATGCATCATTGCAT+AGG	-	tig0045145:1290-1309	Msa1465310:intergenic	45.0%
!	AAGACTGGCTCACCTTTATG+AGG	+	tig0045145:15-34	Msa1465310:CDS	45.0%
	AACTCGCAAACAAGGCCTAA+GGG	-	tig0045145:101-120	Msa1465310:intergenic	45.0%
!!	GTTTGCGAGTTGGATTTTGG+AGG	+	tig0045145:110-129	Msa1465310:intron	45.0%
!!	TTGCGAGTTGGATTTTGGAG+GGG	+	tig0045145:112-131	Msa1465310:intron	45.0%
!!	GTTTGTGAGTTGGGTTTTGG+AGG	-	tig0045145:295-314	Msa1465310:intergenic	45.0%
!!	CTAAGGCTTGGTTTGTGAGT+TGG	-	tig0045145:305-324	Msa1465310:intergenic	45.0%
!!	GTTTGTGAGTTGGGTTTTGG+AGG	-	tig0045145:295-314	Msa1465310:intergenic	45.0%
!!	CTAAGGCTTGGTTTGTGAGT+TGG	-	tig0045145:305-324	Msa1465310:intergenic	45.0%
	ATGTGCAATGCAAGCCTTTG+CGG	-	tig0045145:402-421	Msa1465310:intergenic	45.0%
	GCCTAACAAGAAGGTAAGAG+TGG	+	tig0045145:622-641	Msa1465310:intron	45.0%
!	TGAAGAGTCTAAGGTGGTGA+GGG	+	tig0045145:810-829	Msa1465310:CDS	45.0%
	ACCAGTTGATCCTACAAAGC+TGG	+	tig0045145:837-856	Msa1465310:CDS	45.0%
!	ACCAGCTTTGTAGGATCAAC+TGG	-	tig0045145:841-860	Msa1465310:intergenic	45.0%
	TGCTGCAGGAACTGTTTGAA+GGG	-	tig0045145:914-933	Msa1465310:intergenic	45.0%
!	GCTACATGTTTTTCTGCTGC+AGG	-	tig0045145:928-947	Msa1465310:intergenic	45.0%
!!!	GGATTGGGTTGGTTTGGTTT+TGG	-	tig0045145:1215-1234	Msa1465310:intergenic	45.0%
	CATTGCATAGGTGCATTGGA+AGG	-	tig0045145:1278-1297	Msa1465310:intergenic	45.0%
	GCATCATTGCATAGGTGCAT+TGG	-	tig0045145:1282-1301	Msa1465310:intergenic	45.0%
	TGCACCTATGCAATGATGCA+TGG	+	tig0045145:1283-1302	Msa1465310:three_prime_UTR	45.0%
	GCACCTATGCAATGATGCAT+GGG	+	tig0045145:1284-1303	Msa1465310:three_prime_UTR	45.0%
	TCTCCCATGCATCATTGCAT+AGG	-	tig0045145:1290-1309	Msa1465310:intergenic	45.0%
	CAACTCGCAAACAAGGCCTA+AGG	-	tig0045145:102-121	Msa1465310:intergenic	50.0%
!!	CTTAGGCCTTGTTTGCGAGT+TGG	+	tig0045145:100-119	Msa1465310:intron	50.0%
!!	GAGTTGGATTTTGGAGGGGA+GGG	+	tig0045145:116-135	Msa1465310:intron	50.0%
	TTGCATTGCACATGCCGTTG+AGG	+	tig0045145:409-428	Msa1465310:intron	50.0%
	ACATGCCGTTGAGGTTGAGT+AGG	+	tig0045145:418-437	Msa1465310:intron	50.0%
	AGGCTCCAGTGAAGAGTCTA+AGG	+	tig0045145:801-820	Msa1465310:CDS	50.0%
	CTCCAGTGAAGAGTCTAAGG+TGG	+	tig0045145:804-823	Msa1465310:CDS	50.0%
	CACCACCTTAGACTCTTCAC+TGG	-	tig0045145:809-828	Msa1465310:intergenic	50.0%
!	GTGAAGAGTCTAAGGTGGTG+AGG	+	tig0045145:809-828	Msa1465310:CDS	50.0%
!	GAAGAGTCTAAGGTGGTGAG+GGG	+	tig0045145:811-830	Msa1465310:CDS	50.0%
	GCTGCAGGAACTGTTTGAAG+GGG	-	tig0045145:913-932	Msa1465310:intergenic	50.0%
	CTGCTGCAGGAACTGTTTGA+AGG	-	tig0045145:915-934	Msa1465310:intergenic	50.0%
	GATGCATGGGAGAGCTGTAT+AGG	+	tig0045145:1297-1316	Msa1465310:three_prime_UTR	50.0%
	CAACTCGCAAACAAGGCCTA+AGG	-	tig0045145:102-121	Msa1465310:intergenic	50.0%
!!	CTTAGGCCTTGTTTGCGAGT+TGG	+	tig0045145:100-119	Msa1465310:intron	50.0%
!!	GAGTTGGATTTTGGAGGGGA+GGG	+	tig0045145:116-135	Msa1465310:intron	50.0%
	TTGCATTGCACATGCCGTTG+AGG	+	tig0045145:409-428	Msa1465310:intron	50.0%
	ACATGCCGTTGAGGTTGAGT+AGG	+	tig0045145:418-437	Msa1465310:intron	50.0%
	AGGCTCCAGTGAAGAGTCTA+AGG	+	tig0045145:801-820	Msa1465310:CDS	50.0%
	CTCCAGTGAAGAGTCTAAGG+TGG	+	tig0045145:804-823	Msa1465310:CDS	50.0%
	CACCACCTTAGACTCTTCAC+TGG	-	tig0045145:809-828	Msa1465310:intergenic	50.0%
!	GTGAAGAGTCTAAGGTGGTG+AGG	+	tig0045145:809-828	Msa1465310:CDS	50.0%
!	GAAGAGTCTAAGGTGGTGAG+GGG	+	tig0045145:811-830	Msa1465310:CDS	50.0%
	GCTGCAGGAACTGTTTGAAG+GGG	-	tig0045145:913-932	Msa1465310:intergenic	50.0%
	CTGCTGCAGGAACTGTTTGA+AGG	-	tig0045145:915-934	Msa1465310:intergenic	50.0%
	GATGCATGGGAGAGCTGTAT+AGG	+	tig0045145:1297-1316	Msa1465310:three_prime_UTR	50.0%
!!	CGAGTTGGATTTTGGAGGGG+AGG	+	tig0045145:115-134	Msa1465310:intron	55.0%
!!	CGAGTTGGATTTTGGAGGGG+AGG	+	tig0045145:115-134	Msa1465310:intron	55.0%
!!	TGGATTTTGGAGGGGAGGGC+AGG	+	tig0045145:120-139	Msa1465310:intron	60.0%
!	GAGTCTAAGGTGGTGAGGGG+AGG	+	tig0045145:814-833	Msa1465310:CDS	60.0%
!	GACCAGGGTGCCTGTACTCA+CGG	-	tig0045145:1138-1157	Msa1465310:intergenic	60.0%
!!	TGGATTTTGGAGGGGAGGGC+AGG	+	tig0045145:120-139	Msa1465310:intron	60.0%
!	GAGTCTAAGGTGGTGAGGGG+AGG	+	tig0045145:814-833	Msa1465310:CDS	60.0%
!	GACCAGGGTGCCTGTACTCA+CGG	-	tig0045145:1138-1157	Msa1465310:intergenic	60.0%
!!	GTGGGGGTGTCCGTGAGTAC+AGG	+	tig0045145:1125-1144	Msa1465310:three_prime_UTR	65.0%
	GTCCGTGAGTACAGGCACCC+TGG	+	tig0045145:1133-1152	Msa1465310:three_prime_UTR	65.0%
!!	GTGGGGGTGTCCGTGAGTAC+AGG	+	tig0045145:1125-1144	Msa1465310:three_prime_UTR	65.0%
	GTCCGTGAGTACAGGCACCC+TGG	+	tig0045145:1133-1152	Msa1465310:three_prime_UTR	65.0%

Chromosome	Type	Strat	End	Strand	Name
tig0045145	gene	1	1377	1	ID=Msa1465310;Name=Msa1465310
tig0045145	mRNA	1	1377	1	ID=Msa1465310-mRNA-1;Parent=Msa1465310;Name=Msa1465310-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|1\|1\|1\|0.5\|0.66\|3\|423\|114
tig0045145	exon	1	36	1	ID=Msa1465310-mRNA-1:exon:24750;Parent=Msa1465310-mRNA-1
tig0045145	exon	566	634	566	ID=Msa1465310-mRNA-1:exon:24751;Parent=Msa1465310-mRNA-1
tig0045145	exon	715	1377	715	ID=Msa1465310-mRNA-1:exon:24752;Parent=Msa1465310-mRNA-1
tig0045145	CDS	1	36	1	ID=Msa1465310-mRNA-1:cds;Parent=Msa1465310-mRNA-1
tig0045145	CDS	566	634	566	ID=Msa1465310-mRNA-1:cds;Parent=Msa1465310-mRNA-1
tig0045145	CDS	715	954	715	ID=Msa1465310-mRNA-1:cds;Parent=Msa1465310-mRNA-1
tig0045145	three_prime_UTR	955	1377	955	ID=Msa1465310-mRNA-1:three_prime_utr;Parent=Msa1465310-mRNA-1

Gene Sequence	>Msa1465310 GGAAAAGTACTATTAAGACTGGCTCACCTTTATGAGGTTGGAGAGGACAAAGATTATTCAGTAACCGCAAATGTAGAATTGAAAAAGCTTTTGCCTAACAAGAAGATAAGTAAAGTGACAGAGATGAGTTTGTCTTCTAATCAAGAAAGAGCTGAAATGGAAAAGAAGAGATTAGTTTGGAAGGTAGAAGGAGGCTCCAGTGAAGAGTCTAAGGTGGTGAGGGGAGGACCAGTTGATCCTACAAAGCTGGTTGTTGAACTTGTTCCAATGGAGATTCGGACATTCTTGGTTGACTTTAATCCCCTTCAAACAGTTCCTGCAGCAGAAAAACATGTAGCAATCTAG
Protein sequence	>Msa1465310 GKVLLRLAHLYEVGEDKDYSVTANVELKKLLPNKKISKVTEMSLSSNQERAEMEKKRLVWKVEGGSSEESKVVRGGPVDPTKLVVELVPMEIRTFLVDFNPLQTVPAAEKHVAI*