AlfalfaGEDB | MS.gene99200

M. sativa cultivar XinJiangDaYe / MS.gene99200

KEGG: K14411
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00076.25

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99200.t1	RHN42276.1	98.4	371	5	1	1	370	1	371	3.50E-212	747.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99200.t1	Q8W034	36.6	186	101	2	2	170	4	189	7.4e-30	132.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99200.t1	A0A396GMD3	98.4	371	5	1	1	370	1	371	2.5e-212	747.3

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene99200	0.84716	1.38E-59	-1.69E-46
MS.gene049343	MS.gene99200	0.832703	8.13E-56	-1.69E-46
MS.gene050799	MS.gene99200	0.824807	6.61E-54	-1.69E-46
MS.gene050844	MS.gene99200	0.800282	1.55E-48	-1.69E-46
MS.gene051744	MS.gene99200	0.803853	2.86E-49	-1.69E-46
MS.gene051935	MS.gene99200	0.801643	8.17E-49	-1.69E-46
MS.gene052108	MS.gene99200	0.832453	9.37E-56	-1.69E-46
MS.gene052519	MS.gene99200	0.831158	1.96E-55	-1.69E-46
MS.gene052773	MS.gene99200	0.803343	3.65E-49	-1.69E-46
MS.gene052954	MS.gene99200	0.811461	6.92E-51	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene02655	MS.gene99200	PPI
MS.gene065947	MS.gene99200	PPI
MS.gene31858	MS.gene99200	PPI
MS.gene81954	MS.gene99200	PPI
MS.gene93157	MS.gene99200	PPI
MS.gene99200	MS.gene026672	PPI
MS.gene99200	MS.gene02488	PPI
MS.gene99200	MS.gene33648	PPI
MS.gene99200	MS.gene038047	PPI
MS.gene99200	MS.gene33649	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99200.t1	MTR_5g017280	78.329	383	60	4	1	370	1	373	0.0	582
MS.gene99200.t1	MTR_5g017280	79.837	367	63	3	1	366	1	357	0.0	575
MS.gene99200.t1	MTR_3g055990	36.408	206	115	4	5	197	7	209	1.50e-35	135
MS.gene99200.t1	MTR_3g055990	28.372	215	115	4	91	302	5	183	7.56e-20	91.3
MS.gene99200.t1	MTR_3g055990	36.408	206	115	4	5	197	7	209	1.58e-35	135
MS.gene99200.t1	MTR_3g055990	28.372	215	115	4	91	302	5	183	7.72e-20	90.9
MS.gene99200.t1	MTR_5g097580	36.735	196	108	4	5	187	7	199	8.33e-35	134
MS.gene99200.t1	MTR_5g097580	36.735	196	108	4	5	187	7	199	9.21e-35	133
MS.gene99200.t1	MTR_2g026180	36.408	206	112	5	1	191	1	202	2.02e-34	132
MS.gene99200.t1	MTR_1g073690	34.848	198	108	3	2	180	4	199	1.01e-30	122

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99200.t1	AT4G36960	67.700	387	97	6	3	370	2	379	1.97e-179	503
MS.gene99200.t1	AT4G36960	67.700	387	97	6	3	370	2	379	1.97e-179	503
MS.gene99200.t1	AT5G55550	31.557	244	144	6	5	227	7	248	1.20e-35	135
MS.gene99200.t1	AT5G55550	30.916	262	141	9	91	346	5	232	8.94e-22	96.7
MS.gene99200.t1	AT5G55550	31.557	244	144	6	5	227	7	248	1.20e-35	135
MS.gene99200.t1	AT5G55550	30.916	262	141	9	91	346	5	232	8.94e-22	96.7
MS.gene99200.t1	AT5G55550	31.557	244	144	6	5	227	7	248	1.20e-35	135
MS.gene99200.t1	AT5G55550	30.916	262	141	9	91	346	5	232	8.94e-22	96.7
MS.gene99200.t1	AT5G55550	31.557	244	144	6	5	227	7	248	1.20e-35	135
MS.gene99200.t1	AT5G55550	30.916	262	141	9	91	346	5	232	8.94e-22	96.7

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Find 96 sgRNAs with CRISPR-Local

Find 329 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCATGCTCTCCTAGTA+TGG	0.385185	8.4:-26569624	None:intergenic
AAACCAAAACCTCTATGACC+AGG	0.657073	8.4:-26568179	None:intergenic
AAAGAAACCCATGAACTGGG+GGG	0.745104	8.4:+26566201	MS.gene99200:CDS
AAAGAATATATGAGCAAATT+TGG	0.238823	8.4:+26564254	MS.gene99200:CDS
AAAGGTTACCAGAATATTTG+TGG	0.454043	8.4:+26565195	MS.gene99200:CDS
AAAGTCCTGTGAGGCCTTAT+GGG	0.365563	8.4:+26568816	MS.gene99200:CDS
AAAGTTTCTTCTGTTACTGA+AGG	0.500175	8.4:-26565229	None:intergenic
AACAGATTTATATATGCCAA+AGG	0.623364	8.4:+26565429	MS.gene99200:CDS
AAGAGGTGCGGCTGAATCTA+TGG	0.366406	8.4:-26568705	None:intergenic
AATAGCATGGATTCTTTCAG+GGG	0.553215	8.4:+26568761	MS.gene99200:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGGTTACCAGAATATTTG+TGG	+	chr8.4:26565195-26565214	MS.gene99200:CDS	30.0%
AAAGTTTCTTCTGTTACTGA+AGG	-	chr8.4:26565232-26565251	None:intergenic	30.0%
AACAACAACTAAGCGTTATT+GGG	-	chr8.4:26564392-26564411	None:intergenic	30.0%
AACAAGTTAAATAGAATGCC+GGG	-	chr8.4:26567574-26567593	None:intergenic	30.0%
AATCCAAGATGTTCTCAATA+CGG	+	chr8.4:26567536-26567555	MS.gene99200:intron	30.0%
ACAGGACTTTCATCAAAATA+AGG	-	chr8.4:26568806-26568825	None:intergenic	30.0%
ACCAATGACAAAACCATAAA+AGG	-	chr8.4:26566803-26566822	None:intergenic	30.0%
ACTAGTATCGTGATAATCAT+TGG	+	chr8.4:26565840-26565859	MS.gene99200:intron	30.0%
ATGCATCGTTCATAAAAGTA+TGG	+	chr8.4:26566379-26566398	MS.gene99200:intron	30.0%
ATGCTTTGCTTGTAAATTGA+AGG	+	chr8.4:26565150-26565169	MS.gene99200:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	26564088	26569632	26564088	ID=MS.gene99200
chr8.4	mRNA	26564088	26569632	26564088	ID=MS.gene99200.t1;Parent=MS.gene99200
chr8.4	exon	26564088	26564108	26564088	ID=MS.gene99200.t1.exon1;Parent=MS.gene99200.t1
chr8.4	CDS	26564088	26564108	26564088	ID=cds.MS.gene99200.t1;Parent=MS.gene99200.t1
chr8.4	exon	26564215	26564304	26564215	ID=MS.gene99200.t1.exon2;Parent=MS.gene99200.t1
chr8.4	CDS	26564215	26564304	26564215	ID=cds.MS.gene99200.t1;Parent=MS.gene99200.t1
chr8.4	exon	26564827	26564892	26564827	ID=MS.gene99200.t1.exon3;Parent=MS.gene99200.t1
chr8.4	CDS	26564827	26564892	26564827	ID=cds.MS.gene99200.t1;Parent=MS.gene99200.t1
chr8.4	exon	26564986	26565051	26564986	ID=MS.gene99200.t1.exon4;Parent=MS.gene99200.t1
chr8.4	CDS	26564986	26565051	26564986	ID=cds.MS.gene99200.t1;Parent=MS.gene99200.t1
chr8.4	exon	26565172	26565257	26565172	ID=MS.gene99200.t1.exon5;Parent=MS.gene99200.t1
chr8.4	CDS	26565172	26565257	26565172	ID=cds.MS.gene99200.t1;Parent=MS.gene99200.t1
chr8.4	exon	26565405	26565450	26565405	ID=MS.gene99200.t1.exon6;Parent=MS.gene99200.t1
chr8.4	CDS	26565405	26565450	26565405	ID=cds.MS.gene99200.t1;Parent=MS.gene99200.t1
chr8.4	exon	26565664	26565718	26565664	ID=MS.gene99200.t1.exon7;Parent=MS.gene99200.t1
chr8.4	CDS	26565664	26565718	26565664	ID=cds.MS.gene99200.t1;Parent=MS.gene99200.t1
chr8.4	exon	26566181	26566257	26566181	ID=MS.gene99200.t1.exon8;Parent=MS.gene99200.t1
chr8.4	CDS	26566181	26566257	26566181	ID=cds.MS.gene99200.t1;Parent=MS.gene99200.t1
chr8.4	exon	26567741	26567882	26567741	ID=MS.gene99200.t1.exon9;Parent=MS.gene99200.t1
chr8.4	CDS	26567741	26567882	26567741	ID=cds.MS.gene99200.t1;Parent=MS.gene99200.t1
chr8.4	exon	26567965	26568092	26567965	ID=MS.gene99200.t1.exon10;Parent=MS.gene99200.t1
chr8.4	CDS	26567965	26568092	26567965	ID=cds.MS.gene99200.t1;Parent=MS.gene99200.t1
chr8.4	exon	26568167	26568271	26568167	ID=MS.gene99200.t1.exon11;Parent=MS.gene99200.t1
chr8.4	CDS	26568167	26568271	26568167	ID=cds.MS.gene99200.t1;Parent=MS.gene99200.t1
chr8.4	exon	26568701	26568868	26568701	ID=MS.gene99200.t1.exon12;Parent=MS.gene99200.t1
chr8.4	CDS	26568701	26568868	26568701	ID=cds.MS.gene99200.t1;Parent=MS.gene99200.t1
chr8.4	exon	26569570	26569632	26569570	ID=MS.gene99200.t1.exon13;Parent=MS.gene99200.t1
chr8.4	CDS	26569570	26569632	26569570	ID=cds.MS.gene99200.t1;Parent=MS.gene99200.t1

Gene Sequence

>MS.gene99200

ATGGAGCAACGAAAGCTTGTGGTTAGTGATCAATGTTAAATTTTGTTTATTTATTCTTATTCTTTTATATGATCAATGTTAAAAAATTGAAATTTAACTTATTATGTTCATGAAAATTGTATATTAGGTTTTGGGTATCCCATGGGATGTTGATACTGATGGGTTGAAAGAATATATGAGCAAATTTGGTGAGCTAGAAGATTGTATTGTTATGAAGGTTAGATATTGCATTTTTTTTTTTCACTTATTGTTAAGTATCCGTGACGAAACATTATGGCGAAATTTGATCAATGTAGCCGACCCCAATAACGCTTAGTTGTTGTTATTAAGTATTTAATATATAAATTGGGAGGTGTTAAATAATTGGGAGTATTTTCTTCTTCGGGGGACGTTGTCTCCTACTCTCGTATAGAATCTCCTATTCCGTTGCAACTGAAGCCTTATCACACTCAATATATAAATCACGATCTTTACATAGTCTGGAGATAAAATGCGACATGCTTATCCATGTTTCATGGTGCTAAACAAACAGAGAAAAGATGAAAGTGAGAAATAATAGAAAAAGAATCGAAATGAACTTATTGTTATTTTCTTTGGGACATTTTCTGTGGAATATAAAATTTAATTGCATTATACAGCATTGATTTAGTGATTACTCTATACTGCTAAATTGCCTTTTTGCTTTTAAATTTCGAGTTTACTGGTGTCATATTTTAATTTATTTATGTTTGTATTTTAGGAACGGTCAACTGGCCGATCTCGCGGTTTTGGATATGTGACATTCGTATCGGTGGATGATGCTAAGGTAACTGTTCTTCACACAAAAACATGCATATCTAGTAAATGAGAAGGTAGCTTGAATATGCTTAATCACAAGTTGCATGATTTCTAAATGCAGAATGTGCTATCCAGTGAACACAGTCTTGGCGACAGGACGCTGGAAGTCAAAGTGGCCACACCAAAGGTTTTAACGAATGAAATTTAAAGCCGTGATTATTTACTACTAGTCTAGCTAATGCTGGTCTGCTTTTAAGTTATACGGGGTATGGTCTGATTCTTAACATGCTTTGCTTGTAAATTGAAGGAGGAGATGAGGGCACCAGTCAAAAAGGTTACCAGAATATTTGTGGCCAGGATTGCTCCTTCAGTAACAGAAGAAACTTTTAGGAGGTATATCGAGTGTGATATCTTCATTAAAATAATTTGTAGTATTTCTTCTTTGGGGACGTAGTCTCATACTCTCATATAGAATCTCCGATTCCGTTGAAATGGAAGCATTATTTTAACATTTTTTTTTTCTCTTTTTTTCCTTTGCAGTCATTTTGAGAAATACGGTGATATAACAGATTTATATATGCCAAAGGTACTTTCCTCACTTTGATGGTCAAACATCTAAAGTTAAAATGTGGTTGGTATGTTTTATGCATGCTGTTCACGTATACACATAACATATTGTCAATTGCAGTTGTCACTCAGTTATACGATTACACTGCATTATTATGCATATATATATGATATGTTTATTTAGTTGACGTTTATTTTTGATTCTAGACATTTTATCATTTATTTCCAACAGGATCGTGGATCAAAAACACATCGAGGAATTGGTTTTATCACTTTCGCAACTGCAGGTTTGGCCGTCACTATTTGTCTGTGGTACAATTATCTGCATATTTTAACTTATAGATTTATCATATCTTAATTATCCTCAAATTTAATTTAGAATCGTAGATAATTAATTAGTATTAGTTAACTAGTATCGTGATAATCATTGGCAGAAGACAATTTTGTGAGTACTTCTGAGTTCTGAGACAGGGCTGCTATGCCTTTTCTCTCTGTATTTCTATACATCAATAACACAGAGGGTGTATCATTAACATTCCAGCCACAGTTTTCACTGGACTTGAAATTTATTTTTACACAGAAACATGCATCTTACTATCTTTGTTTATGTTGAATATTGAACTTATATTTATTTTATGGGACATGTTGTTGGCTTTTCGAATTGTAATTATGAGTTACTTGGTTAAACTTTACTGCATTGTTCATAAGAATCAGTAATTTAATCTGTAAGGATGATATTTGCGTTGCAGATTCTGTGGAGAACTTGATGAAAGAAACCCATGAACTGGGGGGTTCTGATGTGGTGGTTGATCGAGCAACACCTAAGGTGATTGTTTAGAGCTTATTCTTTTTACTAAAGAACAGTTTTGTAAAAAATTCCTTATTAATGTTTTCTACTGTTGTATTTTCATTGCATATTCTATTGAAATGATAATCTGTTTGTAGCCATGCATCGTTCATAAAAGTATGGCTTTATGATCACAGTATAAAGATACTTGACCCAATAGTTCAGAACAATAGATTGATAGATATTAAGAGAAAAATAAAATAGAAAAACGATAACTCATCGATAGACAGGCCGGGTTTTTAAAAAGGACAAAAAATTTAAAGTTAATTAAGGAAAGATTTTGGGGAGGATATGTAATATGGATACTCTCCTTGGGAGATCATATTTGAAAATCAAGAAGTCTTTCGAAAAATTAAGATGTTATCTTGATGAGATGAAGTCAAAGAATCAGTCAACATGGTGTGCAAATAAATACTGAGGGAGATGTAAACACTATAGCATAAGAAGAGTCAATTGTATAGTTTGTATTTTATAACTGGTTCAGCTTCCCTTTTTACTCTGGGCATACATTTGGCGTATCCCCTTTTATGGTTTTGTCATTGGTATGAATATCTTGATTTGTCTAGTTATGTCTCAAACTAGATATCTGTCAGAGATATCATTATCTGTTTATTAGCCTGGCTTTTTAGAAAATGATTTATTTATTTTACTGTTATCTCCTTTAATAAGTTATGAGTTAACTGTTTAATCTATTGGATTGAATCATGGACATTTAATGTTTATGAGATTGATGGAAAATGATGAAATAGAGTGAATTGGAATTGAACTATTAATTATTTGATGTTCTATAGTTTAGATTGTCTAAAATAGGATGAAATGGAATGGAACATGATAAAATGGATTCATTCCATCCAATATCCCACTTTTTTCCCCTCCAATTCGAGTGAAGTGAAATGAAGAAAATTTTTAATTCTATCTTCAAAATATCTTGAGTCAACAAAAAATCTTTAAAATATCTGAATAACGAAAGTATGAAAGGCAAACCAAACTGGAATATTTGTTTAGCTTCATTATTATCTTACTAATCTGTAATTTAGTCTTTGGTCTTTACCTGTTACTTCTTCACTTGTAAATATCTTCATTCATTTTATTGTCGTGTTCTATCTTATTTGAAAACATTGTATTATATTTCTTCATTGTTGATTATCTATGATGCACTGGTGGTACTTTATTTTCCCCTCATGTGTCATACTAGACATGCTTCAATATGCTGTCGACACACGTTGGAGCAGTATCAGACTTTTCTTAAAAAAAAAAAATCCAAGATGTTCTCAATACGGCGTCAACACACACCCGGCATTCTATTTAACTTGTTGTAGAAAATATCAATACTACCCGTTTCACACCACCAGTTTTCATTTTCATGCATAAGTCTTTGTTTAGTGATGGAAAGATACCTGATTATAGTCATACATGCCTTTTCTTTTTACTTTTGTTAATAAGCTGTATTCCATTTTGGCAGGAAGATGATTTCAAGCCAATTGGCAGAACCAGAACACCACAATTAGGATATAGTGCATATAGTACTTATATTCCTGCATCAACTAGATATGCAGCACTTGGCGCTCCAACTATGTATGATCGTCCAAGTTCTATTTATGGAAGTAAGCATGGAAACACTGATATTAAATTAAAATTATTAATTCGTAAGTAGCTACATACTGACTGCTTTGAACTGTCAATTAGGAGGAGAACCTGCCCGTGGGATAAGCAAAAAGATTTTTGTTGGTCGTCTTCCCCCGGAGGCAACCACTGAAGATCTTCGCCTGTATTTTGGAAGATTTGGCCATATCTTAGATGTTTATATTCCTAGGGTGAGATTAAGATGTCAAATAAAACATATTCAACTGTTGAAAGCTGGCTAACCCATCTCTCTATTTTGATCTAGGATGTCAAGAGACCTGGTCATAGAGGTTTTGGTTTTGTGACTTTTGCGGACAGTGGTGTTGCAGATCGTGTCTCTCGAAGACCTCATGAGATTTGTGGACACGAGGTACTTTGATTTGGATTTTCTGTTACTTTGCATCATGACCCTACTTGAATTGAATCTACATTTCCTCGTTTGGAAATGGCATTGAAATAAGTGGATGTATTTAAGATTCAACAACCTCCCCCCTCCTTACTGCTTGCAGTGATCTACTTGATATTACATCAGTGTGCAGTGATTGCACTTAATAATCACAACAGACATGTACAGTAGACCTACATGATTCCTGACAGCGCAAATTTCTTTTGACATAACATGTCGAAAAATGTCCAGATGGTCTACAAAGATGATTATGTTTATTGTGTATGCACATTTGTTTAAGACTGCGTAAATTCCTTGTTCTGAAACTTGACTTACCATGTATTCCAAGCGTAATTGTATGTGTCAATCTTAGATTTATAAATGACTTTATTTTTGTGAATCATGCTCCTGCAGGTTGCCATAGATTCAGCCGCACCTCTTGATGAAGCAGGTCCCAGCGGGAATACTATGTTGAATAGCATGGATTCTTTCAGGGGATATGGTGGTCCAGTGAGGCCTTATTTTGATGAAAGTCCTGTGAGGCCTTATGGGAGAATGTACGATAGCCTGGACTTCGATGATGTGAGTATAGACTATATATTCATTATCATTATTTGCATTGCTGAGAGCTTGTGAGAAATTGACAGTAAGCTGAAGAAAACTGGAGAGATTTGAAAGAATCTTTAATTTGTACTTTCTGAAATCTGAAGACTTGGCTTACAAACTGATTATATATAGTACATTATTTATAGATCAAGTTGATAGTTATACTTAACTGTTAGATTCTGTCACTGACAGAATCTGATTACTAAGCAACAATAAGTTCATCCTGACACACTAAAGTATAGTTGGTTATAGGCCCAGTCTGCTAACCTGTTAGCCAAAGTTCCGTTTTGCTAACAGAAGCAGGAGAAGAAATACAATTAAATAGCAGAAATTCAAATTGAGAAATATACTTATAATACACATGAATGGTTTTATAACTAATACGGAAAAGAAATGGTAAATTAGGTAATTTCTCTAGAATTTACCCATTTTATCTTTCTTTGGAATCTACTAGATCCTTAATATGTGTTGTTTTCCATCGTACTCATCTTGCAAGTGAACTGAAGTGCGTGTTTTTTTTTTTTTTAATCATTATGAAAAAGATGGATGATATATTATTTTGTAGTGTCCCTTGCATGTAAAATGAATGGACAATAAAATGGAATCCCGAATCTAGTCGCATGCTTCTTGCTTTTGGTTATTATTCTTACTTTAGTTGCTGTTTATTTATTTAGCAGTTGGGTTATGGAGTTGCTAGCAGAAGACCATCAAGAACAGATTGGAGGTATAGACCATACTAG

Protein sequence

>MS.gene99200.t1

MEQRKLVVLGIPWDVDTDGLKEYMSKFGELEDCIVMKERSTGRSRGFGYVTFVSVDDAKNVLSSEHSLGDRTLEVKVATPKEEMRAPVKKVTRIFVARIAPSVTEETFRSHFEKYGDITDLYMPKDRGSKTHRGIGFITFATADSVENLMKETHELGGSDVVVDRATPKEDDFKPIGRTRTPQLGYSAYSTYIPASTRYAALGAPTMYDRPSSIYGRGEPARGISKKIFVGRLPPEATTEDLRLYFGRFGHILDVYIPRDVKRPGHRGFGFVTFADSGVADRVSRRPHEICGHEVAIDSAAPLDEAGPSGNTMLNSMDSFRGYGGPVRPYFDESPVRPYGRMYDSLDFDDLGYGVASRRPSRTDWRYRPY