Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45785.t1 | XP_003605397.2 | 98.4 | 443 | 7 | 0 | 1 | 443 | 1 | 443 | 8.30E-245 | 855.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45785.t1 | Q9FKV1 | 73.1 | 449 | 96 | 3 | 1 | 443 | 3 | 432 | 2.2e-182 | 639.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45785.t1 | G7JKS8 | 98.4 | 443 | 7 | 0 | 1 | 443 | 1 | 443 | 6.0e-245 | 855.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049166 | MS.gene45785 | 0.832185 | 1.09E-55 | -1.69E-46 |
| MS.gene04917 | MS.gene45785 | 0.801035 | 1.09E-48 | -1.69E-46 |
| MS.gene049887 | MS.gene45785 | 0.808098 | 3.66E-50 | -1.69E-46 |
| MS.gene050114 | MS.gene45785 | 0.82087 | 5.46E-53 | -1.69E-46 |
| MS.gene050317 | MS.gene45785 | 0.804756 | 1.85E-49 | -1.69E-46 |
| MS.gene050838 | MS.gene45785 | 0.863096 | 3.18E-64 | -1.69E-46 |
| MS.gene051003 | MS.gene45785 | 0.827 | 1.99E-54 | -1.69E-46 |
| MS.gene051233 | MS.gene45785 | 0.823293 | 1.50E-53 | -1.69E-46 |
| MS.gene051234 | MS.gene45785 | 0.818064 | 2.38E-52 | -1.69E-46 |
| MS.gene051850 | MS.gene45785 | 0.807472 | 4.97E-50 | -1.69E-46 |
| MS.gene051902 | MS.gene45785 | 0.804541 | 2.06E-49 | -1.69E-46 |
| MS.gene052342 | MS.gene45785 | 0.803199 | 3.91E-49 | -1.69E-46 |
| MS.gene052434 | MS.gene45785 | 0.807474 | 4.97E-50 | -1.69E-46 |
| MS.gene052818 | MS.gene45785 | -0.80567 | 1.19E-49 | -1.69E-46 |
| MS.gene053058 | MS.gene45785 | 0.838331 | 3.06E-57 | -1.69E-46 |
| MS.gene053109 | MS.gene45785 | 0.805375 | 1.38E-49 | -1.69E-46 |
| MS.gene053786 | MS.gene45785 | 0.820549 | 6.47E-53 | -1.69E-46 |
| MS.gene053895 | MS.gene45785 | -0.830195 | 3.37E-55 | -1.69E-46 |
| MS.gene055424 | MS.gene45785 | 0.80606 | 9.88E-50 | -1.69E-46 |
| MS.gene056097 | MS.gene45785 | 0.831107 | 2.01E-55 | -1.69E-46 |
| MS.gene056181 | MS.gene45785 | 0.807397 | 5.16E-50 | -1.69E-46 |
| MS.gene056752 | MS.gene45785 | 0.862569 | 4.62E-64 | -1.69E-46 |
| MS.gene056992 | MS.gene45785 | 0.811953 | 5.41E-51 | -1.69E-46 |
| MS.gene057094 | MS.gene45785 | 0.811405 | 7.12E-51 | -1.69E-46 |
| MS.gene057452 | MS.gene45785 | -0.812478 | 4.15E-51 | -1.69E-46 |
| MS.gene05781 | MS.gene45785 | 0.820755 | 5.80E-53 | -1.69E-46 |
| MS.gene058054 | MS.gene45785 | 0.805641 | 1.21E-49 | -1.69E-46 |
| MS.gene058074 | MS.gene45785 | 0.80903 | 2.31E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene45785 | 0.824649 | 7.20E-54 | -1.69E-46 |
| MS.gene058638 | MS.gene45785 | 0.800532 | 1.38E-48 | -1.69E-46 |
| MS.gene058639 | MS.gene45785 | 0.809015 | 2.33E-50 | -1.69E-46 |
| MS.gene058720 | MS.gene45785 | 0.805395 | 1.36E-49 | -1.69E-46 |
| MS.gene058758 | MS.gene45785 | 0.809092 | 2.25E-50 | -1.69E-46 |
| MS.gene05880 | MS.gene45785 | 0.819515 | 1.12E-52 | -1.69E-46 |
| MS.gene059171 | MS.gene45785 | 0.808803 | 2.59E-50 | -1.69E-46 |
| MS.gene060563 | MS.gene45785 | 0.863896 | 1.79E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene45785 | MS.gene84825 | PPI |
| MS.gene45785 | MS.gene044005 | PPI |
| MS.gene013003 | MS.gene45785 | PPI |
| MS.gene45785 | MS.gene67151 | PPI |
| MS.gene45785 | MS.gene67091 | PPI |
| MS.gene45785 | MS.gene062762 | PPI |
| MS.gene45785 | MS.gene056796 | PPI |
| MS.gene067063 | MS.gene45785 | PPI |
| MS.gene45785 | MS.gene023903 | PPI |
| MS.gene45785 | MS.gene019648 | PPI |
| MS.gene24365 | MS.gene45785 | PPI |
| MS.gene45785 | MS.gene48483 | PPI |
| MS.gene45785 | MS.gene054624 | PPI |
| MS.gene019649 | MS.gene45785 | PPI |
| MS.gene45785 | MS.gene51840 | PPI |
| MS.gene57998 | MS.gene45785 | PPI |
| MS.gene45785 | MS.gene044004 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45785.t1 | MTR_4g030900 | 98.420 | 443 | 7 | 0 | 1 | 443 | 1 | 443 | 0.0 | 881 |
| MS.gene45785.t1 | MTR_7g077830 | 37.838 | 444 | 246 | 6 | 2 | 443 | 119 | 534 | 9.00e-93 | 290 |
| MS.gene45785.t1 | MTR_8g030600 | 36.712 | 444 | 251 | 5 | 2 | 443 | 173 | 588 | 4.55e-90 | 285 |
| MS.gene45785.t1 | MTR_3g463080 | 37.923 | 443 | 247 | 7 | 2 | 443 | 101 | 516 | 2.88e-87 | 275 |
| MS.gene45785.t1 | MTR_8g030600 | 35.838 | 346 | 203 | 4 | 2 | 345 | 173 | 501 | 7.40e-65 | 217 |
| MS.gene45785.t1 | MTR_4g116030 | 32.287 | 446 | 268 | 12 | 2 | 439 | 105 | 524 | 8.26e-63 | 212 |
| MS.gene45785.t1 | MTR_7g066780 | 31.066 | 441 | 277 | 6 | 4 | 442 | 91 | 506 | 5.17e-61 | 206 |
| MS.gene45785.t1 | MTR_5g069630 | 28.409 | 440 | 291 | 7 | 7 | 443 | 97 | 515 | 1.59e-51 | 182 |
| MS.gene45785.t1 | MTR_2g027710 | 43.030 | 165 | 93 | 1 | 231 | 395 | 71 | 234 | 5.30e-41 | 147 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45785.t1 | AT5G44370 | 72.460 | 443 | 109 | 1 | 1 | 443 | 3 | 432 | 0.0 | 649 |
| MS.gene45785.t1 | AT4G00370 | 36.036 | 444 | 254 | 5 | 2 | 443 | 126 | 541 | 5.33e-89 | 281 |
| MS.gene45785.t1 | AT2G29650 | 35.892 | 443 | 256 | 6 | 2 | 443 | 97 | 512 | 6.22e-86 | 272 |
| MS.gene45785.t1 | AT2G29650 | 36.019 | 422 | 240 | 7 | 24 | 443 | 7 | 400 | 1.22e-80 | 255 |
| MS.gene45785.t1 | AT2G29650 | 35.971 | 417 | 237 | 7 | 29 | 443 | 2 | 390 | 2.91e-79 | 251 |
| MS.gene45785.t1 | AT5G20380 | 31.839 | 446 | 266 | 11 | 2 | 439 | 93 | 508 | 1.06e-61 | 209 |
| MS.gene45785.t1 | AT5G20380 | 31.839 | 446 | 266 | 11 | 2 | 439 | 145 | 560 | 3.37e-61 | 209 |
| MS.gene45785.t1 | AT2G38060 | 30.090 | 442 | 282 | 7 | 4 | 442 | 95 | 512 | 9.91e-59 | 201 |
| MS.gene45785.t1 | AT2G29650 | 35.535 | 318 | 188 | 5 | 2 | 318 | 97 | 398 | 4.70e-57 | 193 |
| MS.gene45785.t1 | AT3G46980 | 29.545 | 440 | 287 | 7 | 7 | 443 | 111 | 530 | 2.14e-55 | 192 |
| MS.gene45785.t1 | AT3G46980 | 29.545 | 440 | 287 | 7 | 7 | 443 | 111 | 530 | 2.75e-55 | 192 |
| MS.gene45785.t1 | AT3G46980 | 29.545 | 440 | 287 | 7 | 7 | 443 | 159 | 578 | 1.04e-54 | 191 |
| MS.gene45785.t1 | AT2G38060 | 29.708 | 377 | 248 | 6 | 4 | 377 | 95 | 457 | 6.87e-49 | 175 |
| MS.gene45785.t1 | AT3G46980 | 30.495 | 364 | 230 | 7 | 83 | 443 | 7 | 350 | 1.52e-45 | 162 |
| MS.gene45785.t1 | AT3G46980 | 30.495 | 364 | 230 | 7 | 83 | 443 | 7 | 350 | 1.52e-45 | 162 |
| MS.gene45785.t1 | AT3G46980 | 30.495 | 364 | 230 | 7 | 83 | 443 | 7 | 350 | 1.59e-45 | 162 |
| MS.gene45785.t1 | AT3G46980 | 30.495 | 364 | 230 | 7 | 83 | 443 | 7 | 350 | 1.59e-45 | 162 |
| MS.gene45785.t1 | AT3G46980 | 28.525 | 305 | 196 | 6 | 141 | 443 | 2 | 286 | 1.60e-29 | 117 |
Find 105 sgRNAs with CRISPR-Local
Find 154 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACACCAGCCTCCAACTTTC+TGG | 0.167095 | 4.1:-69844171 | None:intergenic |
| CCTAGTTTGGTTAAGTTTAA+AGG | 0.174452 | 4.1:+69843403 | MS.gene45785:CDS |
| TTTGACTAGTTTGCCTGTTT+GGG | 0.291056 | 4.1:+69843648 | MS.gene45785:CDS |
| TCACTGCACGTTTACTTGTT+GGG | 0.298817 | 4.1:+69843245 | MS.gene45785:CDS |
| CGAAATAACCGCCGGGAACT+TGG | 0.309025 | 4.1:-69843120 | None:intergenic |
| AATGTTGTTTCTACCTAGTT+TGG | 0.318339 | 4.1:+69843390 | MS.gene45785:CDS |
| GGCTGCCAAGGCGTCTGATT+TGG | 0.324835 | 4.1:+69844137 | MS.gene45785:CDS |
| TAAGTTAAGTCTTCATGAAA+TGG | 0.325737 | 4.1:+69843753 | MS.gene45785:CDS |
| TGAAGGTAAGGAGGACTATT+AGG | 0.328157 | 4.1:-69842964 | None:intergenic |
| CTTTGTTGATTGGTTGATTC+CGG | 0.329782 | 4.1:-69843056 | None:intergenic |
| TTCAAATACCGCTGGTACTT+TGG | 0.331484 | 4.1:+69844074 | MS.gene45785:CDS |
| GTCACTGCACGTTTACTTGT+TGG | 0.336931 | 4.1:+69843244 | MS.gene45785:CDS |
| CTATCAATCTTCTTATTAAC+CGG | 0.341138 | 4.1:-69843541 | None:intergenic |
| GAAGATTGATAGACGGGTTT+CGG | 0.343350 | 4.1:+69843552 | MS.gene45785:CDS |
| TCCGGCAGCGTCGGCGGCGA+CGG | 0.343534 | 4.1:-69843038 | None:intergenic |
| CTTTGTATGTGTTGATGAAT+TGG | 0.350434 | 4.1:+69843704 | MS.gene45785:CDS |
| GCACCGGAAGCCGAACTCTT+AGG | 0.365889 | 4.1:-69843502 | None:intergenic |
| AACGTGCAGTGACAAGAATT+AGG | 0.367317 | 4.1:-69843234 | None:intergenic |
| TACGCTTGTTCCCAAGTTCC+CGG | 0.371349 | 4.1:+69843109 | MS.gene45785:CDS |
| TGCCGGTGAATCGTTATTGC+CGG | 0.387504 | 4.1:+69843522 | MS.gene45785:CDS |
| ATGTATCTTGGTGCAGCATT+TGG | 0.393453 | 4.1:+69843367 | MS.gene45785:CDS |
| TAGACGGGTTTCGGATCTTA+AGG | 0.394543 | 4.1:+69843561 | MS.gene45785:CDS |
| GCTTGGTAGGGCTGGGTTTG+CGG | 0.408067 | 4.1:+69844002 | MS.gene45785:CDS |
| ACGTGCAGTGACAAGAATTA+GGG | 0.408785 | 4.1:-69843233 | None:intergenic |
| GGGTTTCGGATCTTAAGGTT+GGG | 0.411547 | 4.1:+69843566 | MS.gene45785:CDS |
| GGTACTTTGGCTGGTATTGT+TGG | 0.418203 | 4.1:+69844087 | MS.gene45785:CDS |
| ACTACTAGTGGTATGTATCT+TGG | 0.423422 | 4.1:+69843355 | MS.gene45785:CDS |
| AAGAGTTCGGCTTCCGGTGC+CGG | 0.424410 | 4.1:+69843505 | MS.gene45785:CDS |
| GGTTATTTCGCCCAGAAAAT+CGG | 0.426214 | 4.1:+69843133 | MS.gene45785:CDS |
| GATCCTAAGAGTTCGGCTTC+CGG | 0.428196 | 4.1:+69843499 | MS.gene45785:CDS |
| GAATTGTACCCTTTGTTGAT+TGG | 0.433565 | 4.1:-69843066 | None:intergenic |
| GCTAAAACAGTATGAATTGA+AGG | 0.438231 | 4.1:-69843289 | None:intergenic |
| ATCTTTCGACTCCAGAAAGT+TGG | 0.441510 | 4.1:+69844160 | MS.gene45785:CDS |
| AAGTTAAGTCTTCATGAAAT+GGG | 0.453234 | 4.1:+69843754 | MS.gene45785:CDS |
| TAGTTCTGATCCTAAGAGTT+CGG | 0.454552 | 4.1:+69843492 | MS.gene45785:CDS |
| CCTTTAAACTTAACCAAACT+AGG | 0.454681 | 4.1:-69843403 | None:intergenic |
| CGGGTTTCGGATCTTAAGGT+TGG | 0.458137 | 4.1:+69843565 | MS.gene45785:CDS |
| ATTGTTGGTGTTGATCTCAC+TGG | 0.460568 | 4.1:+69844102 | MS.gene45785:CDS |
| AATACCGCTGGTACTTTGGC+TGG | 0.464982 | 4.1:+69844078 | MS.gene45785:CDS |
| GATATTGCTCCGAGGTATGC+TGG | 0.468252 | 4.1:+69844036 | MS.gene45785:CDS |
| TCCCACCTCATGAAAGATCT+AGG | 0.474761 | 4.1:+69843320 | MS.gene45785:CDS |
| AAGTGGGGTTGGAATTCCTT+GGG | 0.475309 | 4.1:+69843618 | MS.gene45785:CDS |
| TAATAAGAAGATTGATAGAC+GGG | 0.476333 | 4.1:+69843546 | MS.gene45785:CDS |
| GGGGTGAGGCGAAGAAAAGT+GGG | 0.484396 | 4.1:+69843602 | MS.gene45785:CDS |
| AGGACTATTAGGTAACGAAC+TGG | 0.485563 | 4.1:-69842953 | None:intergenic |
| ACCGTCGCCGCCGACGCTGC+CGG | 0.487605 | 4.1:+69843037 | MS.gene45785:CDS |
| CACATGTGAAGGACCATAAG+AGG | 0.488050 | 4.1:-69843183 | None:intergenic |
| TCTGTTTCTTTGACTACTAG+TGG | 0.491370 | 4.1:+69843343 | MS.gene45785:CDS |
| TTACTTGTTGGGGTTGCTCA+AGG | 0.496633 | 4.1:+69843256 | MS.gene45785:CDS |
| AGGTATGCTGGAATTGTGAT+GGG | 0.500300 | 4.1:+69844048 | MS.gene45785:CDS |
| GACTCCAGAAAGTTGGAGGC+TGG | 0.503695 | 4.1:+69844167 | MS.gene45785:CDS |
| CACTGCACGTTTACTTGTTG+GGG | 0.503801 | 4.1:+69843246 | MS.gene45785:CDS |
| TTTGCCTGTTTGGGCTATTG+TGG | 0.504221 | 4.1:+69843657 | MS.gene45785:CDS |
| GAAATAACCGCCGGGAACTT+GGG | 0.505954 | 4.1:-69843119 | None:intergenic |
| GGTTTCGGATCTTAAGGTTG+GGG | 0.509229 | 4.1:+69843567 | MS.gene45785:CDS |
| CAATAACGATTCACCGGCAC+CGG | 0.510862 | 4.1:-69843518 | None:intergenic |
| TTTCGACTCCAGAAAGTTGG+AGG | 0.514699 | 4.1:+69844163 | MS.gene45785:CDS |
| TTAACCACAATAGCCCAAAC+AGG | 0.515483 | 4.1:-69843661 | None:intergenic |
| GAGGTATGCTGGAATTGTGA+TGG | 0.517053 | 4.1:+69844047 | MS.gene45785:CDS |
| AAAGTGGGGTTGGAATTCCT+TGG | 0.520233 | 4.1:+69843617 | MS.gene45785:CDS |
| CGGATTATTTGATTACGAGA+AGG | 0.522309 | 4.1:+69843839 | MS.gene45785:CDS |
| GAAGGGTTATGTCGGTGACT+AGG | 0.528281 | 4.1:+69843857 | MS.gene45785:CDS |
| TTGGTTGATTCCGGCAGCGT+CGG | 0.528660 | 4.1:-69843047 | None:intergenic |
| TGGGTTTGCGGTGAATCATA+TGG | 0.530242 | 4.1:+69844014 | MS.gene45785:CDS |
| GGATTATTTGATTACGAGAA+GGG | 0.530464 | 4.1:+69843840 | MS.gene45785:CDS |
| GACCTAGATCTTTCATGAGG+TGG | 0.535670 | 4.1:-69843322 | None:intergenic |
| AACACAAACATATTAAGATA+AGG | 0.536099 | 4.1:-69843793 | None:intergenic |
| CTGATGTGCAGATGAAGGTA+AGG | 0.545108 | 4.1:-69842976 | None:intergenic |
| AAGATCCAAATCAGACGCCT+TGG | 0.548033 | 4.1:-69844142 | None:intergenic |
| GAGGCGAAGAAAAGTGGGGT+TGG | 0.550060 | 4.1:+69843607 | MS.gene45785:CDS |
| ACCATAAGAGGAATGAAAAG+AGG | 0.550822 | 4.1:-69843171 | None:intergenic |
| GTTGATTCCGGCAGCGTCGG+CGG | 0.552249 | 4.1:-69843044 | None:intergenic |
| AACCGGCAATAACGATTCAC+CGG | 0.554785 | 4.1:-69843524 | None:intergenic |
| ATTCTGTCCACATTCTACTA+CGG | 0.557355 | 4.1:+69843085 | MS.gene45785:CDS |
| GGCGGCGACGGTGTAAGCGA+TGG | 0.564498 | 4.1:-69843026 | None:intergenic |
| ACCTAGATCTTTCATGAGGT+GGG | 0.566365 | 4.1:-69843321 | None:intergenic |
| GAAATTACTTGAGGCTGCCA+AGG | 0.567990 | 4.1:+69844125 | MS.gene45785:CDS |
| GGAATCAACCAATCAACAAA+GGG | 0.574368 | 4.1:+69843058 | MS.gene45785:CDS |
| TGGAAGAAGTGCACATGTGA+AGG | 0.577324 | 4.1:-69843194 | None:intergenic |
| TTTGTTCTTCGGTGGCACTA+GGG | 0.580681 | 4.1:+69843971 | MS.gene45785:CDS |
| GAATATTGGTGGTGTTGTTG+CGG | 0.581435 | 4.1:+69843819 | MS.gene45785:CDS |
| TTAATAAGAAGATTGATAGA+CGG | 0.582928 | 4.1:+69843545 | MS.gene45785:CDS |
| TTCTGGGCGAAATAACCGCC+GGG | 0.584077 | 4.1:-69843127 | None:intergenic |
| GATTACGAGAAGGGTTATGT+CGG | 0.589669 | 4.1:+69843849 | MS.gene45785:CDS |
| CGGAATCAACCAATCAACAA+AGG | 0.596783 | 4.1:+69843057 | MS.gene45785:CDS |
| CTAGTCAAAATCTTCACCCA+AGG | 0.605141 | 4.1:-69843634 | None:intergenic |
| TTGTTGGTGTTGATCTCACT+GGG | 0.609510 | 4.1:+69844103 | MS.gene45785:CDS |
| ATCACAATTCCAGCATACCT+CGG | 0.611975 | 4.1:-69844045 | None:intergenic |
| TTTCTGGGCGAAATAACCGC+CGG | 0.614744 | 4.1:-69843128 | None:intergenic |
| CCACCCGAAGTTCTAAAACT+CGG | 0.621204 | 4.1:-69843940 | None:intergenic |
| GGTATGCTGGAATTGTGATG+GGG | 0.626679 | 4.1:+69844049 | MS.gene45785:CDS |
| TCTCACTGGGAAATTACTTG+AGG | 0.633681 | 4.1:+69844116 | MS.gene45785:CDS |
| ACAGACTGATGTGCAGATGA+AGG | 0.638296 | 4.1:-69842981 | None:intergenic |
| AGCGTAACCGTAGTAGAATG+TGG | 0.642404 | 4.1:-69843092 | None:intergenic |
| GTCTGTTACATAGAACGCGT+CGG | 0.646309 | 4.1:+69842998 | MS.gene45785:CDS |
| ATGTGCAGATGAAGGTAAGG+AGG | 0.651050 | 4.1:-69842973 | None:intergenic |
| GGGTGAGGCGAAGAAAAGTG+GGG | 0.652496 | 4.1:+69843603 | MS.gene45785:CDS |
| TATTTCGCCCAGAAAATCGG+TGG | 0.664311 | 4.1:+69843136 | MS.gene45785:CDS |
| TTATGTCGGTGACTAGGACG+AGG | 0.669317 | 4.1:+69843863 | MS.gene45785:CDS |
| AGCCCTAATTCAAAATACGT+CGG | 0.677489 | 4.1:-69843730 | None:intergenic |
| GTATGCTGGAATTGTGATGG+GGG | 0.680884 | 4.1:+69844050 | MS.gene45785:CDS |
| ACAGACCTAGATCTTTCATG+AGG | 0.704250 | 4.1:-69843325 | None:intergenic |
| AATACCAGCCAAAGTACCAG+CGG | 0.731925 | 4.1:-69844082 | None:intergenic |
| ATCATATGGATATTGCTCCG+AGG | 0.747409 | 4.1:+69844028 | MS.gene45785:CDS |
| GCTTGTTCCCAAGTTCCCGG+CGG | 0.780032 | 4.1:+69843112 | MS.gene45785:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACACAAACATATTAAGATA+AGG | - | chr4.1:69843796-69843815 | None:intergenic | 20.0% |
| !! | TTAATAAGAAGATTGATAGA+CGG | + | chr4.1:69843545-69843564 | MS.gene45785:CDS | 20.0% |
| ! | AAGTTAAGTCTTCATGAAAT+GGG | + | chr4.1:69843754-69843773 | MS.gene45785:CDS | 25.0% |
| ! | CTATCAATCTTCTTATTAAC+CGG | - | chr4.1:69843544-69843563 | None:intergenic | 25.0% |
| ! | TAAGTTAAGTCTTCATGAAA+TGG | + | chr4.1:69843753-69843772 | MS.gene45785:CDS | 25.0% |
| ! | TAATAAGAAGATTGATAGAC+GGG | + | chr4.1:69843546-69843565 | MS.gene45785:CDS | 25.0% |
| !! | ATGTTTGTGTTTTCGAATAT+TGG | + | chr4.1:69843805-69843824 | MS.gene45785:CDS | 25.0% |
| !!! | TTTGGTGTTTTATTTGGTTT+AGG | + | chr4.1:69843467-69843486 | MS.gene45785:CDS | 25.0% |
| CCTAGTTTGGTTAAGTTTAA+AGG | + | chr4.1:69843403-69843422 | MS.gene45785:CDS | 30.0% | |
| CCTTTAAACTTAACCAAACT+AGG | - | chr4.1:69843406-69843425 | None:intergenic | 30.0% | |
| GCTAAAACAGTATGAATTGA+AGG | - | chr4.1:69843292-69843311 | None:intergenic | 30.0% | |
| GGATTATTTGATTACGAGAA+GGG | + | chr4.1:69843840-69843859 | MS.gene45785:CDS | 30.0% | |
| ! | CTTTGTATGTGTTGATGAAT+TGG | + | chr4.1:69843704-69843723 | MS.gene45785:CDS | 30.0% |
| ! | TTTGTGTTTTCGAATATTGG+TGG | + | chr4.1:69843808-69843827 | MS.gene45785:CDS | 30.0% |
| !! | AATGTTGTTTCTACCTAGTT+TGG | + | chr4.1:69843390-69843409 | MS.gene45785:CDS | 30.0% |
| !! | TACCGACGTATTTTGAATTA+GGG | + | chr4.1:69843728-69843747 | MS.gene45785:CDS | 30.0% |
| !! | TTACCGACGTATTTTGAATT+AGG | + | chr4.1:69843727-69843746 | MS.gene45785:CDS | 30.0% |
| !!! | ACAAGAATTAGGGTTTTGTT+TGG | - | chr4.1:69843226-69843245 | None:intergenic | 30.0% |
| !!! | AGAGTTTTTTAGGGTTTGTT+TGG | + | chr4.1:69843449-69843468 | MS.gene45785:CDS | 30.0% |
| !!! | GGTTTGTTTGGTGTTTTATT+TGG | + | chr4.1:69843461-69843480 | MS.gene45785:CDS | 30.0% |
| !!! | GTATTCTTGTTGTTTTCTAC+CGG | + | chr4.1:69844231-69844250 | MS.gene45785:CDS | 30.0% |
| !!! | TATTCTTGTTGTTTTCTACC+GGG | + | chr4.1:69844232-69844251 | MS.gene45785:CDS | 30.0% |
| !!! | TCATACTGTTTTAGCTCAAT+GGG | + | chr4.1:69843297-69843316 | MS.gene45785:CDS | 30.0% |
| !!! | TTCATACTGTTTTAGCTCAA+TGG | + | chr4.1:69843296-69843315 | MS.gene45785:CDS | 30.0% |
| !!! | TTGTTGCTGAGAGTTTTTTA+GGG | + | chr4.1:69843440-69843459 | MS.gene45785:CDS | 30.0% |
| !!! | TTTGTTGCTGAGAGTTTTTT+AGG | + | chr4.1:69843439-69843458 | MS.gene45785:CDS | 30.0% |
| ACCATAAGAGGAATGAAAAG+AGG | - | chr4.1:69843174-69843193 | None:intergenic | 35.0% | |
| ACTACTAGTGGTATGTATCT+TGG | + | chr4.1:69843355-69843374 | MS.gene45785:CDS | 35.0% | |
| AGCCCTAATTCAAAATACGT+CGG | - | chr4.1:69843733-69843752 | None:intergenic | 35.0% | |
| ATTCTGTCCACATTCTACTA+CGG | + | chr4.1:69843085-69843104 | MS.gene45785:CDS | 35.0% | |
| CGGATTATTTGATTACGAGA+AGG | + | chr4.1:69843839-69843858 | MS.gene45785:CDS | 35.0% | |
| CTAAAAACGCATAAAAGTCC+CGG | - | chr4.1:69844204-69844223 | None:intergenic | 35.0% | |
| GAATTGTACCCTTTGTTGAT+TGG | - | chr4.1:69843069-69843088 | None:intergenic | 35.0% | |
| GGAATCAACCAATCAACAAA+GGG | + | chr4.1:69843058-69843077 | MS.gene45785:CDS | 35.0% | |
| TCAGCAACAAAAACAGATTG+AGG | - | chr4.1:69843430-69843449 | None:intergenic | 35.0% | |
| ! | AGGAAGTTTTTGAATACGGT+TGG | + | chr4.1:69843883-69843902 | MS.gene45785:CDS | 35.0% |
| ! | CTTTGTTGATTGGTTGATTC+CGG | - | chr4.1:69843059-69843078 | None:intergenic | 35.0% |
| ! | GACGAGGAAGTTTTTGAATA+CGG | + | chr4.1:69843879-69843898 | MS.gene45785:CDS | 35.0% |
| ! | GGAAGTTTTTGAATACGGTT+GGG | + | chr4.1:69843884-69843903 | MS.gene45785:CDS | 35.0% |
| ! | TCCTCTTTTCATTCCTCTTA+TGG | + | chr4.1:69843170-69843189 | MS.gene45785:CDS | 35.0% |
| ! | TCTGTTTCTTTGACTACTAG+TGG | + | chr4.1:69843343-69843362 | MS.gene45785:CDS | 35.0% |
| !! | TAGTTCTGATCCTAAGAGTT+CGG | + | chr4.1:69843492-69843511 | MS.gene45785:CDS | 35.0% |
| !! | TTTGACTAGTTTGCCTGTTT+GGG | + | chr4.1:69843648-69843667 | MS.gene45785:CDS | 35.0% |
| !!! | AGGGTTTTGTTTGGATCTAA+TGG | - | chr4.1:69843217-69843236 | None:intergenic | 35.0% |
| !!! | ATACCGAGTTTTAGAACTTC+GGG | + | chr4.1:69843937-69843956 | MS.gene45785:CDS | 35.0% |
| !!! | ATTCTTGTTGTTTTCTACCG+GGG | + | chr4.1:69844233-69844252 | MS.gene45785:CDS | 35.0% |
| !!! | GATACCGAGTTTTAGAACTT+CGG | + | chr4.1:69843936-69843955 | MS.gene45785:CDS | 35.0% |
| !!! | TTTATGCGTTTTTAGTTCGC+TGG | + | chr4.1:69844209-69844228 | MS.gene45785:CDS | 35.0% |
| !!! | TTTTGACTAGTTTGCCTGTT+TGG | + | chr4.1:69843647-69843666 | MS.gene45785:CDS | 35.0% |
| AACGTGCAGTGACAAGAATT+AGG | - | chr4.1:69843237-69843256 | None:intergenic | 40.0% | |
| AAGGTTGGGGTTGAGAAAAA+TGG | + | chr4.1:69843580-69843599 | MS.gene45785:CDS | 40.0% | |
| ACAGACCTAGATCTTTCATG+AGG | - | chr4.1:69843328-69843347 | None:intergenic | 40.0% | |
| ACCTAGATCTTTCATGAGGT+GGG | - | chr4.1:69843324-69843343 | None:intergenic | 40.0% | |
| ACGTGCAGTGACAAGAATTA+GGG | - | chr4.1:69843236-69843255 | None:intergenic | 40.0% | |
| AGGACTATTAGGTAACGAAC+TGG | - | chr4.1:69842956-69842975 | None:intergenic | 40.0% | |
| AGGTATGCTGGAATTGTGAT+GGG | + | chr4.1:69844048-69844067 | MS.gene45785:CDS | 40.0% | |
| AGGTTGGGGTTGAGAAAAAT+GGG | + | chr4.1:69843581-69843600 | MS.gene45785:CDS | 40.0% | |
| ATCACAATTCCAGCATACCT+CGG | - | chr4.1:69844048-69844067 | None:intergenic | 40.0% | |
| ATCATATGGATATTGCTCCG+AGG | + | chr4.1:69844028-69844047 | MS.gene45785:CDS | 40.0% | |
| ATCTTTCGACTCCAGAAAGT+TGG | + | chr4.1:69844160-69844179 | MS.gene45785:CDS | 40.0% | |
| CGGAATCAACCAATCAACAA+AGG | + | chr4.1:69843057-69843076 | MS.gene45785:CDS | 40.0% | |
| CTAGTCAAAATCTTCACCCA+AGG | - | chr4.1:69843637-69843656 | None:intergenic | 40.0% | |
| GATTACGAGAAGGGTTATGT+CGG | + | chr4.1:69843849-69843868 | MS.gene45785:CDS | 40.0% | |
| TCAATCAAAAATCCTCTCCC+CGG | - | chr4.1:69844253-69844272 | None:intergenic | 40.0% | |
| TCACTGCACGTTTACTTGTT+GGG | + | chr4.1:69843245-69843264 | MS.gene45785:CDS | 40.0% | |
| TCTCACTGGGAAATTACTTG+AGG | + | chr4.1:69844116-69844135 | MS.gene45785:CDS | 40.0% | |
| TGAAGGTAAGGAGGACTATT+AGG | - | chr4.1:69842967-69842986 | None:intergenic | 40.0% | |
| TTAACCACAATAGCCCAAAC+AGG | - | chr4.1:69843664-69843683 | None:intergenic | 40.0% | |
| TTCAAATACCGCTGGTACTT+TGG | + | chr4.1:69844074-69844093 | MS.gene45785:CDS | 40.0% | |
| ! | ATGTATCTTGGTGCAGCATT+TGG | + | chr4.1:69843367-69843386 | MS.gene45785:CDS | 40.0% |
| ! | GAAGATTGATAGACGGGTTT+CGG | + | chr4.1:69843552-69843571 | MS.gene45785:CDS | 40.0% |
| ! | GGTTATTTCGCCCAGAAAAT+CGG | + | chr4.1:69843133-69843152 | MS.gene45785:CDS | 40.0% |
| !! | ATTGTTGGTGTTGATCTCAC+TGG | + | chr4.1:69844102-69844121 | MS.gene45785:CDS | 40.0% |
| !! | GAATATTGGTGGTGTTGTTG+CGG | + | chr4.1:69843819-69843838 | MS.gene45785:CDS | 40.0% |
| !! | TTGTTGGTGTTGATCTCACT+GGG | + | chr4.1:69844103-69844122 | MS.gene45785:CDS | 40.0% |
| !!! | TGGTGCTGTGTTTTGTTCTT+CGG | + | chr4.1:69843960-69843979 | MS.gene45785:CDS | 40.0% |
| !!! | TTTACGACCACCGATTTTCT+GGG | - | chr4.1:69843146-69843165 | None:intergenic | 40.0% |
| !!! | TTTTACGACCACCGATTTTC+TGG | - | chr4.1:69843147-69843166 | None:intergenic | 40.0% |
| AAAGTGGGGTTGGAATTCCT+TGG | + | chr4.1:69843617-69843636 | MS.gene45785:CDS | 45.0% | |
| AACCGGCAATAACGATTCAC+CGG | - | chr4.1:69843527-69843546 | None:intergenic | 45.0% | |
| AAGATCCAAATCAGACGCCT+TGG | - | chr4.1:69844145-69844164 | None:intergenic | 45.0% | |
| AAGTGGGGTTGGAATTCCTT+GGG | + | chr4.1:69843618-69843637 | MS.gene45785:CDS | 45.0% | |
| ACAGACTGATGTGCAGATGA+AGG | - | chr4.1:69842984-69843003 | None:intergenic | 45.0% | |
| AGCGTAACCGTAGTAGAATG+TGG | - | chr4.1:69843095-69843114 | None:intergenic | 45.0% | |
| ATGTGCAGATGAAGGTAAGG+AGG | - | chr4.1:69842976-69842995 | None:intergenic | 45.0% | |
| CACATGTGAAGGACCATAAG+AGG | - | chr4.1:69843186-69843205 | None:intergenic | 45.0% | |
| CTGATGTGCAGATGAAGGTA+AGG | - | chr4.1:69842979-69842998 | None:intergenic | 45.0% | |
| GAAATTACTTGAGGCTGCCA+AGG | + | chr4.1:69844125-69844144 | MS.gene45785:CDS | 45.0% | |
| GACCTAGATCTTTCATGAGG+TGG | - | chr4.1:69843325-69843344 | None:intergenic | 45.0% | |
| GAGGTATGCTGGAATTGTGA+TGG | + | chr4.1:69844047-69844066 | MS.gene45785:CDS | 45.0% | |
| GGGTTTCGGATCTTAAGGTT+GGG | + | chr4.1:69843566-69843585 | MS.gene45785:CDS | 45.0% | |
| GGTACTTTGGCTGGTATTGT+TGG | + | chr4.1:69844087-69844106 | MS.gene45785:CDS | 45.0% | |
| GGTATGCTGGAATTGTGATG+GGG | + | chr4.1:69844049-69844068 | MS.gene45785:CDS | 45.0% | |
| GGTTGGGGTTGAGAAAAATG+GGG | + | chr4.1:69843582-69843601 | MS.gene45785:CDS | 45.0% | |
| GGTTTCGGATCTTAAGGTTG+GGG | + | chr4.1:69843567-69843586 | MS.gene45785:CDS | 45.0% | |
| GTATGCTGGAATTGTGATGG+GGG | + | chr4.1:69844050-69844069 | MS.gene45785:CDS | 45.0% | |
| GTCACTGCACGTTTACTTGT+TGG | + | chr4.1:69843244-69843263 | MS.gene45785:CDS | 45.0% | |
| GTCTGTTACATAGAACGCGT+CGG | + | chr4.1:69842998-69843017 | MS.gene45785:CDS | 45.0% | |
| GTTGGGGTTGAGAAAAATGG+GGG | + | chr4.1:69843583-69843602 | MS.gene45785:CDS | 45.0% | |
| TATTTCGCCCAGAAAATCGG+TGG | + | chr4.1:69843136-69843155 | MS.gene45785:CDS | 45.0% | |
| TCCCACCTCATGAAAGATCT+AGG | + | chr4.1:69843320-69843339 | MS.gene45785:CDS | 45.0% | |
| TGGGTTTGCGGTGAATCATA+TGG | + | chr4.1:69844014-69844033 | MS.gene45785:CDS | 45.0% | |
| TTTCGACTCCAGAAAGTTGG+AGG | + | chr4.1:69844163-69844182 | MS.gene45785:CDS | 45.0% | |
| TTTGCCTGTTTGGGCTATTG+TGG | + | chr4.1:69843657-69843676 | MS.gene45785:CDS | 45.0% | |
| TTTGTTCTTCGGTGGCACTA+GGG | + | chr4.1:69843971-69843990 | MS.gene45785:CDS | 45.0% | |
| ! | AATACCAGCCAAAGTACCAG+CGG | - | chr4.1:69844085-69844104 | None:intergenic | 45.0% |
| ! | AGGCTGGTGTTTTTCATTCC+GGG | + | chr4.1:69844183-69844202 | MS.gene45785:CDS | 45.0% |
| ! | CACTGCACGTTTACTTGTTG+GGG | + | chr4.1:69843246-69843265 | MS.gene45785:CDS | 45.0% |
| ! | GAGGCTGGTGTTTTTCATTC+CGG | + | chr4.1:69844182-69844201 | MS.gene45785:CDS | 45.0% |
| ! | TAGACGGGTTTCGGATCTTA+AGG | + | chr4.1:69843561-69843580 | MS.gene45785:CDS | 45.0% |
| ! | TGGAAGAAGTGCACATGTGA+AGG | - | chr4.1:69843197-69843216 | None:intergenic | 45.0% |
| ! | TTACTTGTTGGGGTTGCTCA+AGG | + | chr4.1:69843256-69843275 | MS.gene45785:CDS | 45.0% |
| ! | TTTTGTTCTTCGGTGGCACT+AGG | + | chr4.1:69843970-69843989 | MS.gene45785:CDS | 45.0% |
| !! | CCACCCGAAGTTCTAAAACT+CGG | - | chr4.1:69843943-69843962 | None:intergenic | 45.0% |
| !! | TGCTGTGTTTTGTTCTTCGG+TGG | + | chr4.1:69843963-69843982 | MS.gene45785:CDS | 45.0% |
| AACACCAGCCTCCAACTTTC+TGG | - | chr4.1:69844174-69844193 | None:intergenic | 50.0% | |
| AATACCGCTGGTACTTTGGC+TGG | + | chr4.1:69844078-69844097 | MS.gene45785:CDS | 50.0% | |
| ATGGGGGTTTCAAATACCGC+TGG | + | chr4.1:69844066-69844085 | MS.gene45785:CDS | 50.0% | |
| CAATAACGATTCACCGGCAC+CGG | - | chr4.1:69843521-69843540 | None:intergenic | 50.0% | |
| CGGGTTTCGGATCTTAAGGT+TGG | + | chr4.1:69843565-69843584 | MS.gene45785:CDS | 50.0% | |
| GAAATAACCGCCGGGAACTT+GGG | - | chr4.1:69843122-69843141 | None:intergenic | 50.0% | |
| GAAGGGTTATGTCGGTGACT+AGG | + | chr4.1:69843857-69843876 | MS.gene45785:CDS | 50.0% | |
| GATATTGCTCCGAGGTATGC+TGG | + | chr4.1:69844036-69844055 | MS.gene45785:CDS | 50.0% | |
| GATCCTAAGAGTTCGGCTTC+CGG | + | chr4.1:69843499-69843518 | MS.gene45785:CDS | 50.0% | |
| GGTTGAGAAAAATGGGGGTG+AGG | + | chr4.1:69843588-69843607 | MS.gene45785:CDS | 50.0% | |
| TACGCTTGTTCCCAAGTTCC+CGG | + | chr4.1:69843109-69843128 | MS.gene45785:CDS | 50.0% | |
| TGCCGGTGAATCGTTATTGC+CGG | + | chr4.1:69843522-69843541 | MS.gene45785:CDS | 50.0% | |
| TTATGTCGGTGACTAGGACG+AGG | + | chr4.1:69843863-69843882 | MS.gene45785:CDS | 50.0% | |
| TTTCTGGGCGAAATAACCGC+CGG | - | chr4.1:69843131-69843150 | None:intergenic | 50.0% | |
| ! | TTCGGTGGCACTAGGGTTTT+TGG | + | chr4.1:69843978-69843997 | MS.gene45785:CDS | 50.0% |
| !!! | AGGGTTTTTGGCGCTTGGTA+GGG | + | chr4.1:69843990-69844009 | MS.gene45785:CDS | 50.0% |
| !!! | CCGAGTTTTAGAACTTCGGG+TGG | + | chr4.1:69843940-69843959 | MS.gene45785:CDS | 50.0% |
| !!! | TAGGGTTTTTGGCGCTTGGT+AGG | + | chr4.1:69843989-69844008 | MS.gene45785:CDS | 50.0% |
| !!! | TGTTGTTTTCTACCGGGGAG+AGG | + | chr4.1:69844238-69844257 | MS.gene45785:CDS | 50.0% |
| CGAAATAACCGCCGGGAACT+TGG | - | chr4.1:69843123-69843142 | None:intergenic | 55.0% | |
| GACTCCAGAAAGTTGGAGGC+TGG | + | chr4.1:69844167-69844186 | MS.gene45785:CDS | 55.0% | |
| GAGGCGAAGAAAAGTGGGGT+TGG | + | chr4.1:69843607-69843626 | MS.gene45785:CDS | 55.0% | |
| GGGGTGAGGCGAAGAAAAGT+GGG | + | chr4.1:69843602-69843621 | MS.gene45785:CDS | 55.0% | |
| GGGTGAGGCGAAGAAAAGTG+GGG | + | chr4.1:69843603-69843622 | MS.gene45785:CDS | 55.0% | |
| TTCTGGGCGAAATAACCGCC+GGG | - | chr4.1:69843130-69843149 | None:intergenic | 55.0% | |
| ! | TTGGTTGATTCCGGCAGCGT+CGG | - | chr4.1:69843050-69843069 | None:intergenic | 55.0% |
| ! | TTTTGGCGCTTGGTAGGGCT+GGG | + | chr4.1:69843995-69844014 | MS.gene45785:CDS | 55.0% |
| !! | TTTTTGGCGCTTGGTAGGGC+TGG | + | chr4.1:69843994-69844013 | MS.gene45785:CDS | 55.0% |
| !!! | GCACTAGGGTTTTTGGCGCT+TGG | + | chr4.1:69843985-69844004 | MS.gene45785:CDS | 55.0% |
| AAGAGTTCGGCTTCCGGTGC+CGG | + | chr4.1:69843505-69843524 | MS.gene45785:CDS | 60.0% | |
| GCACCGGAAGCCGAACTCTT+AGG | - | chr4.1:69843505-69843524 | None:intergenic | 60.0% | |
| GCTTGGTAGGGCTGGGTTTG+CGG | + | chr4.1:69844002-69844021 | MS.gene45785:CDS | 60.0% | |
| GCTTGTTCCCAAGTTCCCGG+CGG | + | chr4.1:69843112-69843131 | MS.gene45785:CDS | 60.0% | |
| GGGGGTGAGGCGAAGAAAAG+TGG | + | chr4.1:69843601-69843620 | MS.gene45785:CDS | 60.0% | |
| ! | GGCTGCCAAGGCGTCTGATT+TGG | + | chr4.1:69844137-69844156 | MS.gene45785:CDS | 60.0% |
| ! | GTTGATTCCGGCAGCGTCGG+CGG | - | chr4.1:69843047-69843066 | None:intergenic | 65.0% |
| GGCGGCGACGGTGTAAGCGA+TGG | - | chr4.1:69843029-69843048 | None:intergenic | 70.0% | |
| ! | ACCGTCGCCGCCGACGCTGC+CGG | + | chr4.1:69843037-69843056 | MS.gene45785:CDS | 80.0% |
| !!! | TCCGGCAGCGTCGGCGGCGA+CGG | - | chr4.1:69843041-69843060 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 69842941 | 69844272 | 69842941 | ID=MS.gene45785 |
| chr4.1 | mRNA | 69842941 | 69844272 | 69842941 | ID=MS.gene45785.t1;Parent=MS.gene45785 |
| chr4.1 | exon | 69842941 | 69844272 | 69842941 | ID=MS.gene45785.t1.exon1;Parent=MS.gene45785.t1 |
| chr4.1 | CDS | 69842941 | 69844272 | 69842941 | ID=cds.MS.gene45785.t1;Parent=MS.gene45785.t1 |
| Gene Sequence |
| Protein sequence |
