Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049300.t1 | KEH35591.1 | 97.7 | 1008 | 22 | 1 | 61 | 1068 | 18 | 1024 | 0.00E+00 | 1634.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049300.t1 | Q9LIH5 | 29.7 | 634 | 258 | 23 | 471 | 1067 | 193 | 675 | 8.7e-31 | 137.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049300.t1 | A0A072V1M0 | 97.7 | 1008 | 22 | 1 | 61 | 1068 | 18 | 1024 | 0.0e+00 | 1634.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene049300.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene049300 | 0.801544 | 8.57E-49 | -1.69E-46 |
| MS.gene049300 | MS.gene050258 | 0.807186 | 5.72E-50 | -1.69E-46 |
| MS.gene049300 | MS.gene052307 | 0.808293 | 3.33E-50 | -1.69E-46 |
| MS.gene049300 | MS.gene053351 | 0.801872 | 7.33E-49 | -1.69E-46 |
| MS.gene049300 | MS.gene054939 | 0.942093 | 1.32E-101 | -1.69E-46 |
| MS.gene049300 | MS.gene054940 | 0.932392 | 9.12E-95 | -1.69E-46 |
| MS.gene049300 | MS.gene055513 | 0.801681 | 8.03E-49 | -1.69E-46 |
| MS.gene049300 | MS.gene056519 | 0.833072 | 6.58E-56 | -1.69E-46 |
| MS.gene049300 | MS.gene059570 | 0.801807 | 7.56E-49 | -1.69E-46 |
| MS.gene049300 | MS.gene059778 | 0.811973 | 5.35E-51 | -1.69E-46 |
| MS.gene049300 | MS.gene060933 | 0.80716 | 5.79E-50 | -1.69E-46 |
| MS.gene049300 | MS.gene060935 | 0.812069 | 5.10E-51 | -1.69E-46 |
| MS.gene049300 | MS.gene060937 | 0.822176 | 2.72E-53 | -1.69E-46 |
| MS.gene049300 | MS.gene062156 | 0.845912 | 3.02E-59 | -1.69E-46 |
| MS.gene049300 | MS.gene06422 | 0.923808 | 1.63E-89 | -1.69E-46 |
| MS.gene049300 | MS.gene06423 | 0.916123 | 2.60E-85 | -1.69E-46 |
| MS.gene049300 | MS.gene064425 | 0.830643 | 2.62E-55 | -1.69E-46 |
| MS.gene049300 | MS.gene06561 | 0.8182 | 2.22E-52 | -1.69E-46 |
| MS.gene049300 | MS.gene072785 | 0.831495 | 1.62E-55 | -1.69E-46 |
| MS.gene049300 | MS.gene07510 | 0.842141 | 3.10E-58 | -1.69E-46 |
| MS.gene049300 | MS.gene08808 | 0.837107 | 6.32E-57 | -1.69E-46 |
| MS.gene049300 | MS.gene08963 | 0.820385 | 7.05E-53 | -1.69E-46 |
| MS.gene049300 | MS.gene21536 | 0.808289 | 3.33E-50 | -1.69E-46 |
| MS.gene049300 | MS.gene30977 | 0.940707 | 1.47E-100 | -1.69E-46 |
| MS.gene049300 | MS.gene30978 | 0.931478 | 3.56E-94 | -1.69E-46 |
| MS.gene049300 | MS.gene31111 | 0.808129 | 3.60E-50 | -1.69E-46 |
| MS.gene049300 | MS.gene32891 | 0.800977 | 1.12E-48 | -1.69E-46 |
| MS.gene049300 | MS.gene33135 | 0.806481 | 8.06E-50 | -1.69E-46 |
| MS.gene049300 | MS.gene35071 | 0.802971 | 4.36E-49 | -1.69E-46 |
| MS.gene049300 | MS.gene39046 | 0.822972 | 1.78E-53 | -1.69E-46 |
| MS.gene049300 | MS.gene39529 | 0.817711 | 2.86E-52 | -1.69E-46 |
| MS.gene049300 | MS.gene40157 | 0.820268 | 7.50E-53 | -1.69E-46 |
| MS.gene049300 | MS.gene41678 | 0.80025 | 1.57E-48 | -1.69E-46 |
| MS.gene049300 | MS.gene42558 | 0.807068 | 6.06E-50 | -1.69E-46 |
| MS.gene049300 | MS.gene44434 | 0.815263 | 1.01E-51 | -1.69E-46 |
| MS.gene049300 | MS.gene44969 | 0.808056 | 3.74E-50 | -1.69E-46 |
| MS.gene049300 | MS.gene47581 | 0.811458 | 6.93E-51 | -1.69E-46 |
| MS.gene049300 | MS.gene49422 | 0.80517 | 1.52E-49 | -1.69E-46 |
| MS.gene049300 | MS.gene49423 | 0.822256 | 2.61E-53 | -1.69E-46 |
| MS.gene049300 | MS.gene49426 | 0.809788 | 1.59E-50 | -1.69E-46 |
| MS.gene049300 | MS.gene51789 | 0.814262 | 1.69E-51 | -1.69E-46 |
| MS.gene049300 | MS.gene58351 | 0.809332 | 1.99E-50 | -1.69E-46 |
| MS.gene049300 | MS.gene60935 | 0.859285 | 4.60E-63 | -1.69E-46 |
| MS.gene049300 | MS.gene65454 | 0.801424 | 9.07E-49 | -1.69E-46 |
| MS.gene049300 | MS.gene67077 | 0.806546 | 7.80E-50 | -1.69E-46 |
| MS.gene049300 | MS.gene67168 | 0.831721 | 1.42E-55 | -1.69E-46 |
| MS.gene049300 | MS.gene71884 | 0.810536 | 1.10E-50 | -1.69E-46 |
| MS.gene049300 | MS.gene74383 | 0.806916 | 6.52E-50 | -1.69E-46 |
| MS.gene049300 | MS.gene76374 | 0.809982 | 1.45E-50 | -1.69E-46 |
| MS.gene049300 | MS.gene77356 | 0.800575 | 1.35E-48 | -1.69E-46 |
| MS.gene049300 | MS.gene78318 | 0.804375 | 2.23E-49 | -1.69E-46 |
| MS.gene049300 | MS.gene86826 | 0.840633 | 7.73E-58 | -1.69E-46 |
| MS.gene049300 | MS.gene87360 | 0.801035 | 1.09E-48 | -1.69E-46 |
| MS.gene049300 | MS.gene90386 | 0.834951 | 2.23E-56 | -1.69E-46 |
| MS.gene049300 | MS.gene91778 | 0.810495 | 1.12E-50 | -1.69E-46 |
| MS.gene049300 | MS.gene93025 | 0.866093 | 3.67E-65 | -1.69E-46 |
| MS.gene049300 | MS.gene95712 | 0.83582 | 1.34E-56 | -1.69E-46 |
| MS.gene049300 | MS.gene96190 | 0.831607 | 1.52E-55 | -1.69E-46 |
| MS.gene049300 | MS.gene96191 | 0.849939 | 2.34E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049300.t1 | MTR_3g095960 | 97.321 | 1008 | 26 | 1 | 61 | 1068 | 18 | 1024 | 0.0 | 2036 |
| MS.gene049300.t1 | MTR_3g095960 | 97.384 | 994 | 25 | 1 | 75 | 1068 | 1 | 993 | 0.0 | 2012 |
| MS.gene049300.t1 | MTR_8g093480 | 47.881 | 708 | 305 | 16 | 366 | 1068 | 131 | 779 | 0.0 | 559 |
| MS.gene049300.t1 | MTR_8g093480 | 53.543 | 254 | 90 | 6 | 75 | 318 | 1 | 236 | 6.73e-58 | 214 |
| MS.gene049300.t1 | MTR_8g093480 | 47.881 | 708 | 305 | 16 | 366 | 1068 | 88 | 736 | 0.0 | 558 |
| MS.gene049300.t1 | MTR_8g093480 | 56.633 | 196 | 70 | 4 | 133 | 318 | 3 | 193 | 1.59e-49 | 189 |
| MS.gene049300.t1 | MTR_8g093480 | 47.286 | 700 | 305 | 16 | 366 | 1060 | 88 | 728 | 1.14e-179 | 543 |
| MS.gene049300.t1 | MTR_8g093480 | 56.633 | 196 | 70 | 4 | 133 | 318 | 3 | 193 | 2.04e-49 | 188 |
| MS.gene049300.t1 | MTR_8g093480 | 47.286 | 700 | 305 | 16 | 366 | 1060 | 131 | 771 | 4.04e-179 | 543 |
| MS.gene049300.t1 | MTR_8g093480 | 53.543 | 254 | 90 | 6 | 75 | 318 | 1 | 236 | 1.47e-57 | 214 |
| MS.gene049300.t1 | MTR_2g060650 | 45.631 | 103 | 56 | 0 | 965 | 1067 | 1345 | 1447 | 5.94e-21 | 100 |
| MS.gene049300.t1 | MTR_2g014260 | 45.455 | 88 | 48 | 0 | 977 | 1064 | 801 | 888 | 1.90e-16 | 85.1 |
| MS.gene049300.t1 | MTR_2g014260 | 45.455 | 88 | 48 | 0 | 977 | 1064 | 800 | 887 | 1.91e-16 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049300.t1 | AT3G18640 | 35.092 | 436 | 180 | 16 | 670 | 1067 | 305 | 675 | 4.29e-45 | 174 |
| MS.gene049300.t1 | AT3G18640 | 35.092 | 436 | 180 | 16 | 670 | 1067 | 305 | 675 | 4.29e-45 | 174 |
| MS.gene049300.t1 | AT3G18640 | 35.092 | 436 | 180 | 16 | 670 | 1067 | 305 | 675 | 4.29e-45 | 174 |
| MS.gene049300.t1 | AT2G10553 | 73.750 | 80 | 21 | 0 | 988 | 1067 | 2 | 81 | 5.98e-33 | 122 |
| MS.gene049300.t1 | AT3G26850 | 47.312 | 93 | 46 | 1 | 979 | 1068 | 173 | 265 | 7.53e-20 | 91.3 |
| MS.gene049300.t1 | AT3G26850 | 47.312 | 93 | 46 | 1 | 979 | 1068 | 173 | 265 | 7.53e-20 | 91.3 |
| MS.gene049300.t1 | AT2G33835 | 45.882 | 85 | 45 | 1 | 984 | 1067 | 502 | 586 | 5.49e-16 | 83.2 |
Find 264 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCAACTATTCTCACGATTT+TGG | 0.170219 | 3.3:-79591021 | None:intergenic |
| GGAGCCCTCCTCGTGGTTTC+AGG | 0.197192 | 3.3:+79590876 | MS.gene049300:CDS |
| AAACTTGGGTAATTCAATTA+AGG | 0.210347 | 3.3:+79593184 | MS.gene049300:CDS |
| TTCATTGGATCATACTGTTT+TGG | 0.221297 | 3.3:-79593047 | None:intergenic |
| TCATCCCTTAATCTTCTAAT+AGG | 0.222977 | 3.3:-79591886 | None:intergenic |
| TAGTTCAGAATGGGCTTATT+TGG | 0.226634 | 3.3:+79588457 | MS.gene049300:CDS |
| TCCTTCTTATCACTCAAAAT+TGG | 0.250515 | 3.3:-79592210 | None:intergenic |
| CGTGGTTTCAGGTGGGATTC+AGG | 0.255538 | 3.3:+79590887 | MS.gene049300:CDS |
| AAGCAGAATGATACAGTTTC+TGG | 0.256951 | 3.3:+79592057 | MS.gene049300:CDS |
| CATCATCAGGTCCAGCATTT+TGG | 0.268653 | 3.3:-79593367 | None:intergenic |
| CTCATGGGTAGCAGAGTATC+TGG | 0.271167 | 3.3:-79591071 | None:intergenic |
| TTTCATTTCCTTTGTTTGCT+TGG | 0.279484 | 3.3:-79593390 | None:intergenic |
| TGTCTCCTGATTGGAATTAT+AGG | 0.280617 | 3.3:+79592325 | MS.gene049300:CDS |
| TTCTTGTGTCTGCGGAATAT+TGG | 0.281117 | 3.3:-79593558 | None:intergenic |
| GGAGCCCTATTAGAAGATTA+AGG | 0.281152 | 3.3:+79591881 | MS.gene049300:CDS |
| CTCATGGGTAGTAGGATATC+TGG | 0.284857 | 3.3:-79591533 | None:intergenic |
| CCTTACTTGAAGGTGGAAAA+TGG | 0.287692 | 3.3:+79587446 | MS.gene049300:CDS |
| TCATTAGATCTACCACTCTT+TGG | 0.290780 | 3.3:-79591481 | None:intergenic |
| GAACCAAGTATTATGGATTC+AGG | 0.293291 | 3.3:+79592780 | MS.gene049300:CDS |
| CTCATAGGTAGCAGAATATC+TGG | 0.294314 | 3.3:-79591443 | None:intergenic |
| TCATCAAAGGAATCTTATTA+TGG | 0.303424 | 3.3:+79588515 | MS.gene049300:CDS |
| TCTGTTTATGTTCTTCCATA+TGG | 0.306253 | 3.3:-79592362 | None:intergenic |
| ACTCCATTAGTCCAAATAAA+AGG | 0.309217 | 3.3:+79591968 | MS.gene049300:CDS |
| TTCACTAGAGAAAGGGAAAA+TGG | 0.309831 | 3.3:+79591415 | MS.gene049300:CDS |
| TTTGATGAAAATCCCGTAAC+AGG | 0.314545 | 3.3:-79588500 | None:intergenic |
| CTCGTGGTTAGTAGGATATC+TGG | 0.333203 | 3.3:-79591623 | None:intergenic |
| TTTGCTCAGTAGATACTTCC+TGG | 0.335470 | 3.3:-79592746 | None:intergenic |
| TTTAAATTCTCATGAATTGA+AGG | 0.339340 | 3.3:+79592941 | MS.gene049300:CDS |
| CACCGGAGAACCAAGTATTA+TGG | 0.341165 | 3.3:+79592773 | MS.gene049300:CDS |
| TCAAAACACGGTGGTCAATA+AGG | 0.346403 | 3.3:+79593259 | MS.gene049300:CDS |
| TTTGTTTGCTTGGCATCATC+AGG | 0.350045 | 3.3:-79593380 | None:intergenic |
| TTGATGCATGTGATTGAAAT+AGG | 0.355075 | 3.3:-79593002 | None:intergenic |
| GTCGAAATGGTAAACATTGT+AGG | 0.360118 | 3.3:+79591833 | MS.gene049300:CDS |
| TGCTCAATACTATCATTCAT+TGG | 0.363648 | 3.3:-79593062 | None:intergenic |
| CAGGAGTTGATGGTAGAAAA+AGG | 0.366286 | 3.3:+79590906 | MS.gene049300:CDS |
| GGTAGATCTAATGACGAATA+TGG | 0.374619 | 3.3:+79591670 | MS.gene049300:CDS |
| CACGAGGAGGGCTCCGACTC+CGG | 0.375893 | 3.3:-79590868 | None:intergenic |
| ACCAAAATCGTGAGAATAGT+TGG | 0.381560 | 3.3:+79591020 | MS.gene049300:CDS |
| TGGATATGGTAAAGTTTCTA+TGG | 0.385533 | 3.3:+79591207 | MS.gene049300:CDS |
| TGATAAATTAAGACCTGTTA+CGG | 0.389039 | 3.3:+79588487 | MS.gene049300:CDS |
| ATAAGAAGGAAGCTGATCTT+TGG | 0.389807 | 3.3:+79592223 | MS.gene049300:CDS |
| TATGTTAAGAGGAGGGAGAA+GGG | 0.390502 | 3.3:-79586825 | None:intergenic |
| GAAGATTAAGGGATGATAGA+TGG | 0.392675 | 3.3:+79591893 | MS.gene049300:CDS |
| GCCTCTCTGGCTCATATCCT+TGG | 0.394980 | 3.3:+79592885 | MS.gene049300:CDS |
| TGTACAAGTTTGTTAAGCTT+TGG | 0.396843 | 3.3:-79593611 | None:intergenic |
| GGGAGTTCAACGGCAGGATC+TGG | 0.399881 | 3.3:-79593026 | None:intergenic |
| GTGATTGAAATAGGCATTGC+AGG | 0.400850 | 3.3:-79592993 | None:intergenic |
| GCCTTCTCTTGAAGAAAGAA+TGG | 0.400963 | 3.3:-79592703 | None:intergenic |
| ATAGGCAAATCAGTAAATTC+TGG | 0.403354 | 3.3:-79592135 | None:intergenic |
| GCTAGTGGAAATTGTCGAAA+TGG | 0.404283 | 3.3:+79591820 | MS.gene049300:CDS |
| GGACTGTCCTCGACTCTATC+AGG | 0.404783 | 3.3:-79592033 | None:intergenic |
| ATTTACTGATTTGCCTATAA+CGG | 0.405639 | 3.3:+79592140 | MS.gene049300:CDS |
| TGCATTGGCTGAGTTTGTTA+AGG | 0.407254 | 3.3:+79593433 | MS.gene049300:CDS |
| CATCCCTTAATCTTCTAATA+GGG | 0.409509 | 3.3:-79591885 | None:intergenic |
| GATTACGATTCTGATGGATA+TGG | 0.409902 | 3.3:+79591193 | MS.gene049300:CDS |
| GAAACTGTATCATTCTGCTT+TGG | 0.421384 | 3.3:-79592054 | None:intergenic |
| CGGTCCTCTGCAGATGTTGC+TGG | 0.427388 | 3.3:+79588428 | MS.gene049300:CDS |
| TTTATGTTCTTCCATATGGC+TGG | 0.428708 | 3.3:-79592358 | None:intergenic |
| TGCAAAAGGCAGCTGTAGAA+TGG | 0.429741 | 3.3:+79591147 | MS.gene049300:CDS |
| AACTTGGGTAATTCAATTAA+GGG | 0.430799 | 3.3:+79593185 | MS.gene049300:CDS |
| CACGAATCTCTCAGCCTCTC+TGG | 0.435442 | 3.3:+79592872 | MS.gene049300:CDS |
| ATCTACCACTCTTTGGAGAA+TGG | 0.438405 | 3.3:-79591474 | None:intergenic |
| CAATAGGTACAACGGACATT+TGG | 0.441250 | 3.3:+79592286 | MS.gene049300:CDS |
| ATGCATGGTTCCCAGTCAAT+AGG | 0.446023 | 3.3:+79592270 | MS.gene049300:CDS |
| AACATTTGATGATCTCTACT+TGG | 0.448605 | 3.3:-79591925 | None:intergenic |
| GATAAATTAAGACCTGTTAC+GGG | 0.449771 | 3.3:+79588488 | MS.gene049300:CDS |
| TTCACTAGAGAAAGAGAAGT+TGG | 0.452745 | 3.3:+79591712 | MS.gene049300:CDS |
| AGGTGGGATTCAGGAGTTGA+TGG | 0.452955 | 3.3:+79590896 | MS.gene049300:CDS |
| AAAGTTGTTGATAAAGTAAC+GGG | 0.454132 | 3.3:+79593521 | MS.gene049300:CDS |
| TTCATTAGAGAAAGAGAAGA+TGG | 0.454567 | 3.3:+79591325 | MS.gene049300:CDS |
| GAGACCGTCCCACGTATTCC+AGG | 0.457611 | 3.3:+79586623 | MS.gene049300:CDS |
| ATATGTTAAGAGGAGGGAGA+AGG | 0.458248 | 3.3:-79586826 | None:intergenic |
| CCCATTCTTTCTTCAAGAGA+AGG | 0.459974 | 3.3:+79592702 | MS.gene049300:CDS |
| TTCTTATCACTCAAAATTGG+TGG | 0.461477 | 3.3:-79592207 | None:intergenic |
| TTGTCCAACTGCATGGCAGC+TGG | 0.462211 | 3.3:-79592664 | None:intergenic |
| AGAATGGTCCCTGCTCTCAT+AGG | 0.464550 | 3.3:-79591458 | None:intergenic |
| GAAGGTAGGCACAGGGAAGA+TGG | 0.467005 | 3.3:+79591043 | MS.gene049300:CDS |
| GTTGCAGGCATATGTGGAAA+AGG | 0.468566 | 3.3:+79594445 | MS.gene049300:intron |
| GATGGGGTCTTCCAGCCATA+TGG | 0.468810 | 3.3:+79592347 | MS.gene049300:CDS |
| GGTAGCAAGCAAATGCGGTC+TGG | 0.469238 | 3.3:+79588404 | MS.gene049300:CDS |
| ATTTACTTAGATATGTTAAG+AGG | 0.470502 | 3.3:-79586836 | None:intergenic |
| CTAATGAATTCACTAGAGAA+AGG | 0.471067 | 3.3:+79591407 | MS.gene049300:CDS |
| TTATCTCAGACCTTACTTGA+AGG | 0.472306 | 3.3:+79587436 | MS.gene049300:intron |
| GCAAAAGGCAGCTGTAGAAT+GGG | 0.474363 | 3.3:+79591148 | MS.gene049300:CDS |
| ATAAAAGGCTAAGAGATGAT+AGG | 0.475061 | 3.3:+79591983 | MS.gene049300:CDS |
| CGGAATATTGGCCTCCTGGA+TGG | 0.475692 | 3.3:-79593546 | None:intergenic |
| ATCCTGCTACCCATGAGAGC+AGG | 0.476316 | 3.3:+79591269 | MS.gene049300:CDS |
| ATCCTGCTACCCATGAGAGC+AGG | 0.476316 | 3.3:+79591359 | MS.gene049300:CDS |
| ATCCATAATACTTGGTTCTC+CGG | 0.477069 | 3.3:-79592775 | None:intergenic |
| ATCCTACTAACCACGAGAGC+AGG | 0.477279 | 3.3:+79591629 | MS.gene049300:CDS |
| TCCCTGCTCTCATGGGTAGC+AGG | 0.477919 | 3.3:-79591271 | None:intergenic |
| TCCCTGCTCTCATGGGTAGC+AGG | 0.477919 | 3.3:-79591361 | None:intergenic |
| CCCATCCTATAATTCCAATC+AGG | 0.478103 | 3.3:-79592330 | None:intergenic |
| AGGAGCTTCTGAAACCAACT+TGG | 0.478431 | 3.3:+79593453 | MS.gene049300:CDS |
| TTCACTAGTGAAAGGGAAGA+TGG | 0.479255 | 3.3:+79591505 | MS.gene049300:CDS |
| TTCACTAGTGAAAGGGAAGA+TGG | 0.479255 | 3.3:+79591595 | MS.gene049300:CDS |
| ACTTAGATATGTTAAGAGGA+GGG | 0.482899 | 3.3:-79586832 | None:intergenic |
| TAACCCAGGAAGAGAAAACT+TGG | 0.485595 | 3.3:+79593169 | MS.gene049300:CDS |
| CCTTCTCTTGAAGAAAGAAT+GGG | 0.486829 | 3.3:-79592702 | None:intergenic |
| GAGCCCTATTAGAAGATTAA+GGG | 0.488698 | 3.3:+79591882 | MS.gene049300:CDS |
| GACCGCATTTGCTTGCTACC+AGG | 0.490417 | 3.3:-79588401 | None:intergenic |
| CAAAACATGGACCAAAATGC+TGG | 0.493199 | 3.3:+79593356 | MS.gene049300:CDS |
| AGGTACAACGGACATTTGGC+CGG | 0.493224 | 3.3:+79592290 | MS.gene049300:CDS |
| GAAAATGGAACATGGAAAGC+AGG | 0.493394 | 3.3:+79587461 | MS.gene049300:CDS |
| GAGAATAAAAGTGGAAATGT+AGG | 0.496432 | 3.3:+79592102 | MS.gene049300:CDS |
| CTGAAACCAACTTGGAAAGA+AGG | 0.496600 | 3.3:+79593461 | MS.gene049300:CDS |
| GAGAATGGTCCCTGCTCTCA+TGG | 0.498339 | 3.3:-79591279 | None:intergenic |
| GAGAATGGTCCCTGCTCTCA+TGG | 0.498339 | 3.3:-79591369 | None:intergenic |
| TATTCTGTTGTGTTCAGAGA+TGG | 0.499211 | 3.3:-79592421 | None:intergenic |
| TGTCCGTTGTACCTATTGAC+TGG | 0.499259 | 3.3:-79592281 | None:intergenic |
| GCCCTCCTCGTGGTTTCAGG+TGG | 0.499663 | 3.3:+79590879 | MS.gene049300:CDS |
| ATCCTACTACCCATGAGAGC+AGG | 0.503657 | 3.3:+79591539 | MS.gene049300:CDS |
| TCTGCGGAATATTGGCCTCC+TGG | 0.504700 | 3.3:-79593550 | None:intergenic |
| CATAAAAGTACCAAAGAGAA+TGG | 0.505662 | 3.3:+79593329 | MS.gene049300:CDS |
| CTTTGGATAGCAGAAAATAC+TGG | 0.505714 | 3.3:+79592240 | MS.gene049300:CDS |
| ATGCAGAAATGTCTCCTGAT+TGG | 0.505794 | 3.3:+79592316 | MS.gene049300:CDS |
| CCAAAATCGTGAGAATAGTT+GGG | 0.505813 | 3.3:+79591021 | MS.gene049300:CDS |
| TGGTAGCAATAGTTCAGAAT+GGG | 0.505951 | 3.3:+79588448 | MS.gene049300:CDS |
| GAGAATAGTCCCTGCTCTCA+TGG | 0.506671 | 3.3:-79591549 | None:intergenic |
| TCCCTGCTCTCATGGGTAGT+AGG | 0.506862 | 3.3:-79591541 | None:intergenic |
| GAGAATGGTCAACACTCTCA+TGG | 0.506959 | 3.3:-79591087 | None:intergenic |
| TCAGAATGGGCTTATTTGGA+AGG | 0.508080 | 3.3:+79588461 | MS.gene049300:CDS |
| CCCTCCTCGTGGTTTCAGGT+GGG | 0.508118 | 3.3:+79590880 | MS.gene049300:CDS |
| TCCCTGCTCTCGTGGTTAGT+AGG | 0.508353 | 3.3:-79591631 | None:intergenic |
| GAAGGAACATGATGTAAGCC+AGG | 0.509135 | 3.3:+79592383 | MS.gene049300:CDS |
| ATCTACCACTCTTAAGAGAA+TGG | 0.511468 | 3.3:-79591102 | None:intergenic |
| ATCTACCACTCTTAAGAGAA+TGG | 0.511468 | 3.3:-79591294 | None:intergenic |
| ATCTACCACTCTTAAGAGAA+TGG | 0.511468 | 3.3:-79591384 | None:intergenic |
| AAGCCTGAATCCATAATACT+TGG | 0.517448 | 3.3:-79592783 | None:intergenic |
| ATTTGCCTATAACGGATGGA+TGG | 0.518017 | 3.3:+79592148 | MS.gene049300:CDS |
| TGGATGAGTTCACTAGAGAA+AGG | 0.519337 | 3.3:+79591227 | MS.gene049300:CDS |
| AAAGCTTAACAAACTTGTAC+AGG | 0.519903 | 3.3:+79593613 | MS.gene049300:CDS |
| AATCTAATCCATTTGCAGCC+TGG | 0.521014 | 3.3:+79593236 | MS.gene049300:CDS |
| TCCTGATTGGAATTATAGGA+TGG | 0.523046 | 3.3:+79592329 | MS.gene049300:CDS |
| GAATCTTATTATGGAGGCAG+AGG | 0.523186 | 3.3:+79588524 | MS.gene049300:CDS |
| CTAATGAATTCACTAGTGAA+AGG | 0.525451 | 3.3:+79591497 | MS.gene049300:CDS |
| CTAATGAATTCACTAGTGAA+AGG | 0.525451 | 3.3:+79591587 | MS.gene049300:CDS |
| TAAAGTAACGGGTACCATCC+AGG | 0.528168 | 3.3:+79593532 | MS.gene049300:CDS |
| AAGCTGATCGAGAATAAAAG+TGG | 0.529647 | 3.3:+79592093 | MS.gene049300:CDS |
| ATTGTTGACATGAGTGGAGC+TGG | 0.529969 | 3.3:+79588329 | MS.gene049300:CDS |
| ATTCTGCTACCTATGAGAGC+AGG | 0.529969 | 3.3:+79591449 | MS.gene049300:CDS |
| TCATGGGTAGTAGGATATCT+GGG | 0.532080 | 3.3:-79591532 | None:intergenic |
| ACGTGCATTTAAATTTGCAT+TGG | 0.534604 | 3.3:+79593418 | MS.gene049300:CDS |
| TAATGAATTCACTAGAGAAA+GGG | 0.537037 | 3.3:+79591408 | MS.gene049300:CDS |
| TAATGAATTCACTAGTGAAA+GGG | 0.537448 | 3.3:+79591498 | MS.gene049300:CDS |
| TAATGAATTCACTAGTGAAA+GGG | 0.537448 | 3.3:+79591588 | MS.gene049300:CDS |
| TCGTGGTTAGTAGGATATCT+GGG | 0.538819 | 3.3:-79591622 | None:intergenic |
| TCTCAGACCTTACTTGAAGG+TGG | 0.539114 | 3.3:+79587439 | MS.gene049300:intron |
| TTGCCAGGAACAGTCGGAGC+CGG | 0.539425 | 3.3:+79590849 | MS.gene049300:intron |
| CTGGCTCATATCCTTGGAGC+AGG | 0.539793 | 3.3:+79592891 | MS.gene049300:CDS |
| AGAAGAGAATGGGACACTGA+TGG | 0.540407 | 3.3:+79588596 | MS.gene049300:CDS |
| TTGCCTATAACGGATGGATG+GGG | 0.543758 | 3.3:+79592150 | MS.gene049300:CDS |
| ACTGATTTGCCTATAACGGA+TGG | 0.544476 | 3.3:+79592144 | MS.gene049300:CDS |
| GAAAGTTGTTGATAAAGTAA+CGG | 0.545410 | 3.3:+79593520 | MS.gene049300:CDS |
| CTGGTAGCAATAGTTCAGAA+TGG | 0.546984 | 3.3:+79588447 | MS.gene049300:CDS |
| GAAATATAAAGCTTCACTCT+AGG | 0.548013 | 3.3:+79586675 | MS.gene049300:CDS |
| TACTTAGATATGTTAAGAGG+AGG | 0.550609 | 3.3:-79586833 | None:intergenic |
| GGATGAGTTCACTAGAGAAA+GGG | 0.551833 | 3.3:+79591228 | MS.gene049300:CDS |
| GTGCATGATTACGATTCTGA+TGG | 0.551916 | 3.3:+79591187 | MS.gene049300:CDS |
| TTTGCCTATAACGGATGGAT+GGG | 0.552603 | 3.3:+79592149 | MS.gene049300:CDS |
| ATAGTTGGGAAGGTAGGCAC+AGG | 0.554939 | 3.3:+79591035 | MS.gene049300:CDS |
| TAACAATGAAGAGGTAGCTA+AGG | 0.555412 | 3.3:+79593283 | MS.gene049300:CDS |
| AAGGCTAAGAGATGATAGGT+GGG | 0.557111 | 3.3:+79591987 | MS.gene049300:CDS |
| CTTGACTTTGTCCAACTGCA+TGG | 0.557475 | 3.3:-79592671 | None:intergenic |
| CCTGATTGGAATTATAGGAT+GGG | 0.560325 | 3.3:+79592330 | MS.gene049300:CDS |
| GGTACAACGGACATTTGGCC+GGG | 0.563103 | 3.3:+79592291 | MS.gene049300:CDS |
| ACTTCCAGCTGCCATGCAGT+TGG | 0.563914 | 3.3:+79592660 | MS.gene049300:CDS |
| TCCTACTACCCATGAGAGCA+GGG | 0.565414 | 3.3:+79591540 | MS.gene049300:CDS |
| TGTTGATGCTATACACAGCC+AGG | 0.565431 | 3.3:+79592728 | MS.gene049300:CDS |
| AGAATAGTCCCTGCTCTCAT+GGG | 0.565639 | 3.3:-79591548 | None:intergenic |
| ATATGTGGAAAAGGTTCAGA+AGG | 0.567581 | 3.3:+79594454 | MS.gene049300:CDS |
| AGAATGGTCAACACTCTCAT+GGG | 0.572749 | 3.3:-79591086 | None:intergenic |
| GGAACAGTCGGAGCCGGAGT+CGG | 0.575027 | 3.3:+79590855 | MS.gene049300:intron |
| GTGAGAATAGTTGGGAAGGT+AGG | 0.576320 | 3.3:+79591029 | MS.gene049300:CDS |
| AGCTCTATTGTTGACATGAG+TGG | 0.576612 | 3.3:+79588323 | MS.gene049300:CDS |
| GCTGTTGGAAAATGTAGAAG+AGG | 0.576715 | 3.3:+79590968 | MS.gene049300:CDS |
| CTGATTGGAATTATAGGATG+GGG | 0.577469 | 3.3:+79592331 | MS.gene049300:CDS |
| TCTTTGTTCCCTGGAATACG+TGG | 0.577581 | 3.3:-79586632 | None:intergenic |
| AGAATGGTCCCTGCTCTCAT+GGG | 0.581139 | 3.3:-79591278 | None:intergenic |
| AGAATGGTCCCTGCTCTCAT+GGG | 0.581139 | 3.3:-79591368 | None:intergenic |
| TCCTGCTACCCATGAGAGCA+GGG | 0.581326 | 3.3:+79591270 | MS.gene049300:CDS |
| TCCTGCTACCCATGAGAGCA+GGG | 0.581326 | 3.3:+79591360 | MS.gene049300:CDS |
| TCATGGGTAGCAGAGTATCT+GGG | 0.581368 | 3.3:-79591070 | None:intergenic |
| GGAGACATTTCTGCATCACC+CGG | 0.581677 | 3.3:-79592309 | None:intergenic |
| GCAGCTGCTGACCTGCTCCA+AGG | 0.582262 | 3.3:-79592902 | None:intergenic |
| TCAAAGGAATCTTATTATGG+AGG | 0.583027 | 3.3:+79588518 | MS.gene049300:CDS |
| GGCATGGTTTAGATAAGAGC+GGG | 0.583797 | 3.3:+79592172 | MS.gene049300:CDS |
| CAGAAAGCACTCTTCAAAGT+GGG | 0.584050 | 3.3:+79588352 | MS.gene049300:CDS |
| GTCCGTTGTACCTATTGACT+GGG | 0.584696 | 3.3:-79592280 | None:intergenic |
| TGATCATTGCCAGGAACAGT+CGG | 0.587729 | 3.3:+79590843 | MS.gene049300:intron |
| AAAGGCTAAGAGATGATAGG+TGG | 0.588164 | 3.3:+79591986 | MS.gene049300:CDS |
| ATGAAGAGGTAGCTAAGGAA+AGG | 0.588800 | 3.3:+79593288 | MS.gene049300:CDS |
| TCCTACTAACCACGAGAGCA+GGG | 0.589630 | 3.3:+79591630 | MS.gene049300:CDS |
| TTTACTTGGAGGAATGGCTG+GGG | 0.589692 | 3.3:-79593111 | None:intergenic |
| AACAGAATACAACTTGCTCC+AGG | 0.591084 | 3.3:+79592435 | MS.gene049300:CDS |
| AAGCAAACAAAGGAAATGAA+AGG | 0.591168 | 3.3:+79593392 | MS.gene049300:CDS |
| AATACTGGTGCCAACATGCA+TGG | 0.593621 | 3.3:+79592255 | MS.gene049300:CDS |
| TTCTGCTACCTATGAGAGCA+GGG | 0.594783 | 3.3:+79591450 | MS.gene049300:CDS |
| ATCACTCTTGACATGTTCTG+AGG | 0.596680 | 3.3:-79593213 | None:intergenic |
| TCATAGGTAGCAGAATATCT+GGG | 0.598574 | 3.3:-79591442 | None:intergenic |
| TAGTGCGGTTAGGCACATGC+CGG | 0.601071 | 3.3:-79591774 | None:intergenic |
| AGAGAATGGTCCTCAAAACA+TGG | 0.604039 | 3.3:+79593343 | MS.gene049300:CDS |
| GGAGCATCAATAGTGCGGTT+AGG | 0.607218 | 3.3:-79591784 | None:intergenic |
| AATCGTGAGAATAGTTGGGA+AGG | 0.607787 | 3.3:+79591025 | MS.gene049300:CDS |
| TTACTTGGAGGAATGGCTGG+GGG | 0.607949 | 3.3:-79593110 | None:intergenic |
| GCAGAAAGCACTCTTCAAAG+TGG | 0.608415 | 3.3:+79588351 | MS.gene049300:CDS |
| GCAAGCAAATGCGGTCTGGA+CGG | 0.609119 | 3.3:+79588408 | MS.gene049300:CDS |
| CTGTGACAGAATTTCTACTT+CGG | 0.609464 | 3.3:-79593141 | None:intergenic |
| TATGGAAGAACATAAACAGA+AGG | 0.610346 | 3.3:+79592365 | MS.gene049300:CDS |
| TGCAGTTGGACAAAGTCAAG+TGG | 0.611494 | 3.3:+79592674 | MS.gene049300:CDS |
| CTCACGATACGCAAGCATGT+AGG | 0.613211 | 3.3:+79591860 | MS.gene049300:CDS |
| GTTGACCATTCTCTTAAGAG+TGG | 0.613457 | 3.3:+79591097 | MS.gene049300:CDS |
| ATCAGTAAATTCTGGCTCTG+TGG | 0.613647 | 3.3:-79592127 | None:intergenic |
| ACACTGATGGAAGTTACAGC+AGG | 0.614680 | 3.3:+79588609 | MS.gene049300:CDS |
| GAGAAAGAGAAGTTGGCAGA+AGG | 0.615126 | 3.3:+79591719 | MS.gene049300:CDS |
| AGACCGTCCCACGTATTCCA+GGG | 0.615666 | 3.3:+79586624 | MS.gene049300:CDS |
| AAGTAGAGATCATCAAATGT+TGG | 0.616100 | 3.3:+79591927 | MS.gene049300:CDS |
| TGGAACATGGAAAGCAGGGA+GGG | 0.621653 | 3.3:+79587466 | MS.gene049300:CDS |
| CAATAAGGATAACAATGAAG+AGG | 0.623098 | 3.3:+79593274 | MS.gene049300:CDS |
| TGCCTATAACGGATGGATGG+GGG | 0.624741 | 3.3:+79592151 | MS.gene049300:CDS |
| AAGAATAGTCCCTGCTCTCG+TGG | 0.625384 | 3.3:-79591639 | None:intergenic |
| TGAAGGTGGAAAATGGAACA+TGG | 0.627347 | 3.3:+79587453 | MS.gene049300:CDS |
| CTGTCACAGTTGTCTAACCC+AGG | 0.627767 | 3.3:+79593155 | MS.gene049300:CDS |
| TGATGATGCCAAGCAAACAA+AGG | 0.634153 | 3.3:+79593382 | MS.gene049300:CDS |
| TGCAAGGAGCATCAATAGTG+CGG | 0.638490 | 3.3:-79591789 | None:intergenic |
| AAAATGGAACATGGAAAGCA+GGG | 0.638602 | 3.3:+79587462 | MS.gene049300:CDS |
| GATGAGCATAAATTCTCACC+TGG | 0.639966 | 3.3:+79588383 | MS.gene049300:CDS |
| AGGGACTATTCTCTTAAGAG+TGG | 0.644286 | 3.3:+79591559 | MS.gene049300:CDS |
| TGGCAGCTGGAAGTATGCAA+AGG | 0.646432 | 3.3:-79592651 | None:intergenic |
| CAAAAGAATGCTGATGCAAG+TGG | 0.647309 | 3.3:+79593083 | MS.gene049300:CDS |
| GGCTGATCCTGATAGAGTCG+AGG | 0.649107 | 3.3:+79592026 | MS.gene049300:CDS |
| GTTCCCTGGAATACGTGGGA+CGG | 0.649549 | 3.3:-79586627 | None:intergenic |
| CACCTGGTAGCAAGCAAATG+CGG | 0.651152 | 3.3:+79588399 | MS.gene049300:CDS |
| CCAACAGCAAAATCTCTGCA+CGG | 0.656607 | 3.3:-79590953 | None:intergenic |
| AAGGAACATGATGTAAGCCA+GGG | 0.656718 | 3.3:+79592384 | MS.gene049300:CDS |
| AGGGACCATTCTCTTAAGAG+TGG | 0.657090 | 3.3:+79591289 | MS.gene049300:CDS |
| AGGGACCATTCTCTTAAGAG+TGG | 0.657090 | 3.3:+79591379 | MS.gene049300:CDS |
| CCCACCTGAAACCACGAGGA+GGG | 0.658576 | 3.3:-79590880 | None:intergenic |
| TGGAGGCAGAGGTTCAAATG+AGG | 0.658931 | 3.3:+79588535 | MS.gene049300:CDS |
| CTTTGTTCCCTGGAATACGT+GGG | 0.658971 | 3.3:-79586631 | None:intergenic |
| AGGGACCATTCTCCAAAGAG+TGG | 0.659573 | 3.3:+79591469 | MS.gene049300:CDS |
| TTTGCAGCCTGGTCAAAACA+CGG | 0.660051 | 3.3:+79593247 | MS.gene049300:CDS |
| AACCCAGGAAGAGAAAACTT+GGG | 0.660535 | 3.3:+79593170 | MS.gene049300:CDS |
| TGACTGGGAACCATGCATGT+TGG | 0.671378 | 3.3:-79592265 | None:intergenic |
| TCCCACCTGAAACCACGAGG+AGG | 0.671552 | 3.3:-79590881 | None:intergenic |
| TGGCTAGATATGTACCACCC+TGG | 0.672330 | 3.3:-79592401 | None:intergenic |
| TTTAAAGCAGCATATAGCTG+CGG | 0.673413 | 3.3:-79592924 | None:intergenic |
| GCTACCAGCAACATCTGCAG+AGG | 0.681868 | 3.3:-79588432 | None:intergenic |
| TTCCCAGTCAATAGGTACAA+CGG | 0.683573 | 3.3:+79592278 | MS.gene049300:CDS |
| ACTCTTGACATGTTCTGAGG+AGG | 0.684272 | 3.3:-79593210 | None:intergenic |
| TAGTTGGGAAGGTAGGCACA+GGG | 0.685040 | 3.3:+79591036 | MS.gene049300:CDS |
| ACTAGAGAAAGGGAAAATGG+AGG | 0.685470 | 3.3:+79591418 | MS.gene049300:CDS |
| ATGGAACATGGAAAGCAGGG+AGG | 0.687641 | 3.3:+79587465 | MS.gene049300:CDS |
| GAACATGATGTAAGCCAGGG+TGG | 0.694356 | 3.3:+79592387 | MS.gene049300:CDS |
| TATGTGGAAAAGGTTCAGAA+GGG | 0.694565 | 3.3:+79594455 | MS.gene049300:CDS |
| TCCAAGGATATGAGCCAGAG+AGG | 0.701534 | 3.3:-79592886 | None:intergenic |
| TCTACTGAGCAAAAGTACAC+CGG | 0.702328 | 3.3:+79592756 | MS.gene049300:CDS |
| CGGAGTCGGAGCCCTCCTCG+TGG | 0.703718 | 3.3:+79590869 | MS.gene049300:CDS |
| GTGGGGTTCAGATAGTGACA+TGG | 0.704274 | 3.3:+79592005 | MS.gene049300:CDS |
| AGGCTAAGAGATGATAGGTG+GGG | 0.711837 | 3.3:+79591988 | MS.gene049300:CDS |
| GGGCATGGTTTAGATAAGAG+CGG | 0.723162 | 3.3:+79592171 | MS.gene049300:CDS |
| GAATCCCACCTGAAACCACG+AGG | 0.757642 | 3.3:-79590884 | None:intergenic |
| AGTAACGGGTACCATCCAGG+AGG | 0.795570 | 3.3:+79593535 | MS.gene049300:CDS |
| GCAGCCTGGTCAAAACACGG+TGG | 0.812953 | 3.3:+79593250 | MS.gene049300:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 79586593 | 79594481 | 79586593 | ID=MS.gene049300 |
| chr3.3 | mRNA | 79586593 | 79594481 | 79586593 | ID=MS.gene049300.t1;Parent=MS.gene049300 |
| chr3.3 | exon | 79586593 | 79586696 | 79586593 | ID=MS.gene049300.t1.exon1;Parent=MS.gene049300.t1 |
| chr3.3 | CDS | 79586593 | 79586696 | 79586593 | ID=cds.MS.gene049300.t1;Parent=MS.gene049300.t1 |
| chr3.3 | exon | 79586817 | 79586852 | 79586817 | ID=MS.gene049300.t1.exon2;Parent=MS.gene049300.t1 |
| chr3.3 | CDS | 79586817 | 79586852 | 79586817 | ID=cds.MS.gene049300.t1;Parent=MS.gene049300.t1 |
| chr3.3 | exon | 79587445 | 79587487 | 79587445 | ID=MS.gene049300.t1.exon3;Parent=MS.gene049300.t1 |
| chr3.3 | CDS | 79587445 | 79587487 | 79587445 | ID=cds.MS.gene049300.t1;Parent=MS.gene049300.t1 |
| chr3.3 | exon | 79588299 | 79588672 | 79588299 | ID=MS.gene049300.t1.exon4;Parent=MS.gene049300.t1 |
| chr3.3 | CDS | 79588299 | 79588672 | 79588299 | ID=cds.MS.gene049300.t1;Parent=MS.gene049300.t1 |
| chr3.3 | exon | 79590856 | 79592456 | 79590856 | ID=MS.gene049300.t1.exon5;Parent=MS.gene049300.t1 |
| chr3.3 | CDS | 79590856 | 79592456 | 79590856 | ID=cds.MS.gene049300.t1;Parent=MS.gene049300.t1 |
| chr3.3 | exon | 79592616 | 79593634 | 79592616 | ID=MS.gene049300.t1.exon6;Parent=MS.gene049300.t1 |
| chr3.3 | CDS | 79592616 | 79593634 | 79592616 | ID=cds.MS.gene049300.t1;Parent=MS.gene049300.t1 |
| chr3.3 | exon | 79594452 | 79594481 | 79594452 | ID=MS.gene049300.t1.exon7;Parent=MS.gene049300.t1 |
| chr3.3 | CDS | 79594452 | 79594481 | 79594452 | ID=cds.MS.gene049300.t1;Parent=MS.gene049300.t1 |
| Gene Sequence |
| Protein sequence |
