Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21536.t1 | ALM55021.1 | 98.2 | 329 | 6 | 0 | 175 | 503 | 100 | 428 | 2.70E-183 | 651.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21536.t1 | Q8W5B1 | 51.1 | 333 | 127 | 7 | 175 | 503 | 101 | 401 | 4.3e-81 | 303.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21536.t1 | A0A141GTN5 | 98.2 | 329 | 6 | 0 | 175 | 503 | 100 | 428 | 1.9e-183 | 651.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene21536 | 0.852053 | 5.94E-61 | -1.69E-46 |
| MS.gene04918 | MS.gene21536 | 0.838626 | 2.57E-57 | -1.69E-46 |
| MS.gene049254 | MS.gene21536 | 0.837097 | 6.36E-57 | -1.69E-46 |
| MS.gene049300 | MS.gene21536 | 0.808289 | 3.33E-50 | -1.69E-46 |
| MS.gene049350 | MS.gene21536 | 0.81444 | 1.54E-51 | -1.69E-46 |
| MS.gene049368 | MS.gene21536 | 0.829322 | 5.49E-55 | -1.69E-46 |
| MS.gene049499 | MS.gene21536 | 0.821834 | 3.27E-53 | -1.69E-46 |
| MS.gene049672 | MS.gene21536 | 0.814169 | 1.77E-51 | -1.69E-46 |
| MS.gene049711 | MS.gene21536 | 0.8633 | 2.75E-64 | -1.69E-46 |
| MS.gene049807 | MS.gene21536 | 0.818263 | 2.15E-52 | -1.69E-46 |
| MS.gene05045 | MS.gene21536 | 0.807243 | 5.56E-50 | -1.69E-46 |
| MS.gene050559 | MS.gene21536 | 0.824216 | 9.10E-54 | -1.69E-46 |
| MS.gene050561 | MS.gene21536 | 0.816535 | 5.26E-52 | -1.69E-46 |
| MS.gene050562 | MS.gene21536 | 0.815551 | 8.72E-52 | -1.69E-46 |
| MS.gene050828 | MS.gene21536 | 0.800464 | 1.42E-48 | -1.69E-46 |
| MS.gene051019 | MS.gene21536 | 0.808335 | 3.26E-50 | -1.69E-46 |
| MS.gene051279 | MS.gene21536 | 0.811076 | 8.39E-51 | -1.69E-46 |
| MS.gene051744 | MS.gene21536 | 0.874106 | 8.75E-68 | -1.69E-46 |
| MS.gene051975 | MS.gene21536 | 0.812382 | 4.36E-51 | -1.69E-46 |
| MS.gene052072 | MS.gene21536 | 0.822893 | 1.86E-53 | -1.69E-46 |
| MS.gene052307 | MS.gene21536 | 0.831949 | 1.25E-55 | -1.69E-46 |
| MS.gene052463 | MS.gene21536 | 0.82413 | 9.53E-54 | -1.69E-46 |
| MS.gene052516 | MS.gene21536 | 0.810183 | 1.31E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene21536 | 0.8128 | 3.53E-51 | -1.69E-46 |
| MS.gene052533 | MS.gene21536 | 0.845222 | 4.65E-59 | -1.69E-46 |
| MS.gene052609 | MS.gene21536 | 0.817399 | 3.37E-52 | -1.69E-46 |
| MS.gene052773 | MS.gene21536 | 0.801162 | 1.03E-48 | -1.69E-46 |
| MS.gene053079 | MS.gene21536 | 0.8096 | 1.75E-50 | -1.69E-46 |
| MS.gene053226 | MS.gene21536 | 0.828015 | 1.14E-54 | -1.69E-46 |
| MS.gene053272 | MS.gene21536 | -0.808853 | 2.52E-50 | -1.69E-46 |
| MS.gene053296 | MS.gene21536 | 0.803004 | 4.29E-49 | -1.69E-46 |
| MS.gene054142 | MS.gene21536 | 0.81676 | 4.68E-52 | -1.69E-46 |
| MS.gene05423 | MS.gene21536 | 0.819068 | 1.41E-52 | -1.69E-46 |
| MS.gene05458 | MS.gene21536 | 0.83453 | 2.84E-56 | -1.69E-46 |
| MS.gene054651 | MS.gene21536 | 0.860777 | 1.63E-63 | -1.69E-46 |
| MS.gene054709 | MS.gene21536 | 0.8044 | 2.20E-49 | -1.69E-46 |
| MS.gene054939 | MS.gene21536 | 0.810353 | 1.20E-50 | -1.69E-46 |
| MS.gene054940 | MS.gene21536 | 0.800763 | 1.24E-48 | -1.69E-46 |
| MS.gene055386 | MS.gene21536 | 0.806127 | 9.57E-50 | -1.69E-46 |
| MS.gene055513 | MS.gene21536 | 0.822689 | 2.07E-53 | -1.69E-46 |
| MS.gene055586 | MS.gene21536 | 0.805619 | 1.22E-49 | -1.69E-46 |
| MS.gene056050 | MS.gene21536 | 0.829419 | 5.20E-55 | -1.69E-46 |
| MS.gene056366 | MS.gene21536 | 0.814197 | 1.74E-51 | -1.69E-46 |
| MS.gene056699 | MS.gene21536 | 0.856588 | 2.91E-62 | -1.69E-46 |
| MS.gene05686 | MS.gene21536 | 0.878401 | 2.89E-69 | -1.69E-46 |
| MS.gene05690 | MS.gene21536 | 0.819283 | 1.26E-52 | -1.69E-46 |
| MS.gene057034 | MS.gene21536 | 0.812 | 5.28E-51 | -1.69E-46 |
| MS.gene057082 | MS.gene21536 | 0.819242 | 1.29E-52 | -1.69E-46 |
| MS.gene057102 | MS.gene21536 | 0.882893 | 7.11E-71 | -1.69E-46 |
| MS.gene057132 | MS.gene21536 | 0.812573 | 3.96E-51 | -1.69E-46 |
| MS.gene057208 | MS.gene21536 | 0.817511 | 3.17E-52 | -1.69E-46 |
| MS.gene057611 | MS.gene21536 | 0.821519 | 3.87E-53 | -1.69E-46 |
| MS.gene058008 | MS.gene21536 | 0.815308 | 9.88E-52 | -1.69E-46 |
| MS.gene058009 | MS.gene21536 | 0.802564 | 5.29E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene21536 | 0.814887 | 1.23E-51 | -1.69E-46 |
| MS.gene058781 | MS.gene21536 | 0.81884 | 1.59E-52 | -1.69E-46 |
| MS.gene058783 | MS.gene21536 | 0.819483 | 1.13E-52 | -1.69E-46 |
| MS.gene05894 | MS.gene21536 | 0.817716 | 2.85E-52 | -1.69E-46 |
| MS.gene058974 | MS.gene21536 | 0.803411 | 3.53E-49 | -1.69E-46 |
| MS.gene059015 | MS.gene21536 | 0.801981 | 6.97E-49 | -1.69E-46 |
| MS.gene059570 | MS.gene21536 | 0.814699 | 1.35E-51 | -1.69E-46 |
| MS.gene059625 | MS.gene21536 | 0.819212 | 1.31E-52 | -1.69E-46 |
| MS.gene059634 | MS.gene21536 | 0.810481 | 1.13E-50 | -1.69E-46 |
| MS.gene059778 | MS.gene21536 | 0.820905 | 5.36E-53 | -1.69E-46 |
| MS.gene05985 | MS.gene21536 | -0.833839 | 4.24E-56 | -1.69E-46 |
| MS.gene060338 | MS.gene21536 | 0.802434 | 5.62E-49 | -1.69E-46 |
| MS.gene060525 | MS.gene21536 | 0.824672 | 7.11E-54 | -1.69E-46 |
| MS.gene060933 | MS.gene21536 | 0.825228 | 5.26E-54 | -1.69E-46 |
| MS.gene060935 | MS.gene21536 | 0.860699 | 1.72E-63 | -1.69E-46 |
| MS.gene060937 | MS.gene21536 | 0.860426 | 2.08E-63 | -1.69E-46 |
| MS.gene06094 | MS.gene21536 | 0.818311 | 2.09E-52 | -1.69E-46 |
| MS.gene060962 | MS.gene21536 | 0.808512 | 2.99E-50 | -1.69E-46 |
| MS.gene061100 | MS.gene21536 | 0.818575 | 1.82E-52 | -1.69E-46 |
| MS.gene061160 | MS.gene21536 | 0.812391 | 4.34E-51 | -1.69E-46 |
| MS.gene061188 | MS.gene21536 | 0.841807 | 3.80E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21536.t1 | MTR_5g013150 | 97.872 | 329 | 7 | 0 | 175 | 503 | 150 | 478 | 0.0 | 673 |
| MS.gene21536.t1 | MTR_1g090240 | 41.520 | 171 | 95 | 1 | 329 | 494 | 496 | 666 | 1.90e-36 | 143 |
| MS.gene21536.t1 | MTR_1g090240 | 41.584 | 101 | 49 | 3 | 175 | 271 | 255 | 349 | 2.62e-11 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21536.t1 | AT4G16845 | 52.778 | 324 | 115 | 8 | 174 | 492 | 40 | 330 | 3.66e-93 | 288 |
| MS.gene21536.t1 | AT4G16845 | 52.632 | 323 | 115 | 8 | 175 | 492 | 101 | 390 | 1.02e-91 | 286 |
| MS.gene21536.t1 | AT5G51230 | 53.125 | 320 | 119 | 4 | 175 | 494 | 337 | 625 | 5.03e-90 | 288 |
| MS.gene21536.t1 | AT5G51230 | 53.125 | 320 | 119 | 4 | 175 | 494 | 337 | 625 | 5.03e-90 | 288 |
| MS.gene21536.t1 | AT5G51230 | 52.484 | 322 | 123 | 5 | 175 | 494 | 337 | 630 | 6.59e-90 | 288 |
| MS.gene21536.t1 | AT2G35670 | 35.849 | 265 | 151 | 6 | 237 | 490 | 471 | 727 | 1.85e-43 | 164 |
| MS.gene21536.t1 | AT4G16810 | 49.045 | 157 | 74 | 4 | 343 | 495 | 2 | 156 | 1.68e-42 | 153 |
Find 140 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAACAACCGTTTCAAATTT+TGG | 0.069545 | 5.3:-6176449 | MS.gene21536:CDS |
| GGGTCCCACGCGAGGGATTC+AGG | 0.191554 | 5.3:-6176222 | MS.gene21536:CDS |
| ATTCATCCCGAACTTTATTT+TGG | 0.206594 | 5.3:-6168561 | MS.gene21536:intron |
| ATCTATTCAACTTTGAGTTC+TGG | 0.236833 | 5.3:-6173919 | MS.gene21536:CDS |
| TATATTGATTTGCTACATTC+AGG | 0.264863 | 5.3:+6172119 | None:intergenic |
| TACTTTCCAGATGAAGTTTC+CGG | 0.273998 | 5.3:-6176655 | MS.gene21536:CDS |
| CCGAGAGGCTCCGTTCAAAA+TGG | 0.288773 | 5.3:-6173008 | MS.gene21536:CDS |
| TGACATATTCTCCCCTTTGC+TGG | 0.299644 | 5.3:+6172298 | None:intergenic |
| GGAAATGGTGGGGTAAAATT+TGG | 0.311328 | 5.3:-6172239 | MS.gene21536:CDS |
| GAACAACTGCAGTATAATAT+TGG | 0.316221 | 5.3:-6168138 | MS.gene21536:CDS |
| AATTTGAAACGGTTGTTATT+CGG | 0.326035 | 5.3:+6176453 | None:intergenic |
| GTGAATTCGTCGTCGGAGTC+AGG | 0.341571 | 5.3:-6176560 | MS.gene21536:CDS |
| TCTATTCAACTTTGAGTTCT+GGG | 0.342392 | 5.3:-6173918 | MS.gene21536:intron |
| TATTGGAAAACTACCAAAAC+GGG | 0.359120 | 5.3:-6168121 | MS.gene21536:CDS |
| CAGACCGTTCAGACTCAAAA+AGG | 0.365583 | 5.3:-6172052 | MS.gene21536:intron |
| AGATTACCCTTCTATCTTCC+AGG | 0.369139 | 5.3:+6169415 | None:intergenic |
| ATGGTCAAACTTTGGAATCA+TGG | 0.374400 | 5.3:-6168182 | MS.gene21536:CDS |
| TTCAGGGTCCTCAGAAGATT+GGG | 0.379052 | 5.3:+6172136 | None:intergenic |
| CAATGTAAATTTCTTGAAGT+TGG | 0.379544 | 5.3:-6172976 | MS.gene21536:CDS |
| AATTCACCGGTGCATAATTC+CGG | 0.383881 | 5.3:-6179375 | MS.gene21536:CDS |
| ACTCCATTCTGTATGCCTTC+TGG | 0.388728 | 5.3:+6172948 | None:intergenic |
| AAAAGCAGCTCATGCATCTC+TGG | 0.388906 | 5.3:-6168812 | MS.gene21536:CDS |
| TTTAACTTCTTTGCTTTAGT+AGG | 0.389750 | 5.3:+6172077 | None:intergenic |
| GATTATTCATGGTCAAACTT+TGG | 0.391223 | 5.3:-6168190 | MS.gene21536:CDS |
| ATGAGTACCAAAATAAAGTT+CGG | 0.391721 | 5.3:+6168554 | None:intergenic |
| AATCACAAACCTTCTTCTTC+TGG | 0.397340 | 5.3:-6173142 | MS.gene21536:intron |
| CTTTCACTCACACAGAGTTC+AGG | 0.399813 | 5.3:-6170016 | MS.gene21536:intron |
| TTGCTAACCTGGAAGATAGA+AGG | 0.399917 | 5.3:-6169422 | MS.gene21536:CDS |
| TATTCTCCCCTTTGCTGGAC+AGG | 0.412431 | 5.3:+6172303 | None:intergenic |
| GAGAGTGATGTCGGAGATGA+AGG | 0.419366 | 5.3:-6176322 | MS.gene21536:CDS |
| AAGAAGAAGGTTTGTGATTG+TGG | 0.419388 | 5.3:+6173146 | None:intergenic |
| ATCACCGCCGTCTCCGACGC+CGG | 0.434220 | 5.3:+6176288 | None:intergenic |
| ATTCAGGGTCCTCAGAAGAT+TGG | 0.434866 | 5.3:+6172135 | None:intergenic |
| AGAGATAAAGATTCGGCGTC+GGG | 0.435204 | 5.3:-6176531 | MS.gene21536:CDS |
| CAGGTGTTGGAGATTATTCA+TGG | 0.435769 | 5.3:-6168201 | MS.gene21536:CDS |
| TGAGTACCAAAATAAAGTTC+GGG | 0.440705 | 5.3:+6168555 | None:intergenic |
| AGCTGTGATTTCTTCTTCAC+CGG | 0.443162 | 5.3:+6179356 | None:intergenic |
| GAGAAGAATTTGCAGAATGA+GGG | 0.451580 | 5.3:-6172260 | MS.gene21536:CDS |
| TGAGAAGAATTTGCAGAATG+AGG | 0.452340 | 5.3:-6172261 | MS.gene21536:CDS |
| TCCATGGCACTAGGATGATC+AGG | 0.457194 | 5.3:+6172215 | None:intergenic |
| GAAAGAACTGCCTCTTCTGC+AGG | 0.458363 | 5.3:+6170034 | None:intergenic |
| GGGATCCGATGAGGAAATGA+TGG | 0.464225 | 5.3:-6176251 | MS.gene21536:CDS |
| TGGTCAAACTTTGGAATCAT+GGG | 0.467268 | 5.3:-6168181 | MS.gene21536:CDS |
| CGAGAGGCTCCGTTCAAAAT+GGG | 0.471224 | 5.3:-6173007 | MS.gene21536:CDS |
| TTTGCTTTAGTAGGCAGAGC+AGG | 0.471731 | 5.3:+6172086 | None:intergenic |
| ATTCCGCCGGAAACTTCATC+TGG | 0.474719 | 5.3:+6176649 | None:intergenic |
| ATATTGATTTGCTACATTCA+GGG | 0.478951 | 5.3:+6172120 | None:intergenic |
| TTACAGAGACGTTCACTGCT+TGG | 0.482117 | 5.3:+6173799 | None:intergenic |
| ATATTGGAAAACTACCAAAA+CGG | 0.483344 | 5.3:-6168122 | MS.gene21536:CDS |
| GTTGGAAGGGAGAGTGATGT+CGG | 0.488539 | 5.3:-6176331 | MS.gene21536:CDS |
| GTGGCCAAGGATGGAGGTGT+TGG | 0.491062 | 5.3:-6176349 | MS.gene21536:intron |
| TAGATATCCTGTCCAGCAAA+GGG | 0.491609 | 5.3:-6172310 | MS.gene21536:CDS |
| AACACTTGTTCCAGTGCCAT+GGG | 0.492904 | 5.3:+6169483 | None:intergenic |
| TTGCAGAATGAGGGAAATGG+TGG | 0.494933 | 5.3:-6172251 | MS.gene21536:CDS |
| GGCTGATGGTCATGTTCCGT+GGG | 0.496542 | 5.3:-6168623 | MS.gene21536:CDS |
| CGGTGATGATGCGACGGCGG+AGG | 0.502344 | 5.3:-6176272 | MS.gene21536:CDS |
| GACATCGGTGGCAGTGGAGA+GGG | 0.502710 | 5.3:-6176591 | MS.gene21536:CDS |
| GGAGGAGAGAGATAAAGATT+CGG | 0.505642 | 5.3:-6176538 | MS.gene21536:CDS |
| GGTGATGATGCGACGGCGGA+GGG | 0.506486 | 5.3:-6176271 | MS.gene21536:CDS |
| CAACTTCAAGAAATTTACAT+TGG | 0.507439 | 5.3:+6172977 | None:intergenic |
| GGTCCCACGCGAGGGATTCA+GGG | 0.509260 | 5.3:-6176221 | MS.gene21536:CDS |
| GACGGCGACATCGGTGGCAG+TGG | 0.511975 | 5.3:-6176597 | MS.gene21536:CDS |
| GACTACGCCGGCGTCGGAGA+CGG | 0.512752 | 5.3:-6176295 | MS.gene21536:CDS |
| AGAATACCCAAAATTTGAAA+CGG | 0.514717 | 5.3:+6176442 | None:intergenic |
| TGAATTCGTCGTCGGAGTCA+GGG | 0.514998 | 5.3:-6176559 | MS.gene21536:CDS |
| TAACACTTGTTCCAGTGCCA+TGG | 0.516004 | 5.3:+6169482 | None:intergenic |
| TCCTGATCATCCTAGTGCCA+TGG | 0.516526 | 5.3:-6172216 | MS.gene21536:CDS |
| TGATGATGGAGTTGCTAACC+TGG | 0.518682 | 5.3:-6169433 | MS.gene21536:CDS |
| TGGCTGATGGTCATGTTCCG+TGG | 0.518749 | 5.3:-6168624 | MS.gene21536:CDS |
| TAAAGATTCGGCGTCGGGAG+CGG | 0.520432 | 5.3:-6176526 | MS.gene21536:CDS |
| GAGAGATAAAGATTCGGCGT+CGG | 0.524477 | 5.3:-6176532 | MS.gene21536:CDS |
| AGGGGAGTGGCCAAGGATGG+AGG | 0.527763 | 5.3:-6176355 | MS.gene21536:intron |
| TTGAAGTTGGATTCACCAGA+AGG | 0.532834 | 5.3:-6172963 | MS.gene21536:CDS |
| CCAAGGATGGAGGTGTTGGA+AGG | 0.534364 | 5.3:-6176345 | MS.gene21536:intron |
| GAAGATTGAGGCGGTTCCGT+CGG | 0.536556 | 5.3:-6176682 | MS.gene21536:intron |
| GGGCGCGTGGGTCCCACGCG+AGG | 0.536764 | 5.3:-6176230 | MS.gene21536:CDS |
| AGTGTGATCGACGGCGACAT+CGG | 0.538138 | 5.3:-6176606 | MS.gene21536:CDS |
| GTCGTATGCTCCTGCAGAAG+AGG | 0.539504 | 5.3:-6170044 | MS.gene21536:CDS |
| GTTAATGAAGCCCATGGCAC+TGG | 0.540871 | 5.3:-6169493 | MS.gene21536:intron |
| TTGAAGAATTCAACATGTGC+CGG | 0.544153 | 5.3:-6179401 | None:intergenic |
| CGCGCCCATCATTTCCTCAT+CGG | 0.548187 | 5.3:+6176246 | None:intergenic |
| ATCGGTGGCAGTGGAGAGGG+TGG | 0.548671 | 5.3:-6176588 | MS.gene21536:CDS |
| AACATGTTCCGCGTAATCCA+TGG | 0.551776 | 5.3:+6172199 | None:intergenic |
| CAAGGATGGAGGTGTTGGAA+GGG | 0.553342 | 5.3:-6176344 | MS.gene21536:intron |
| AGATTCGGCGTCGGGAGCGG+AGG | 0.559422 | 5.3:-6176523 | MS.gene21536:CDS |
| CATGTGCCGGCAAAATTCAC+CGG | 0.559627 | 5.3:-6179388 | MS.gene21536:CDS |
| TCACCAGAAGGCATACAGAA+TGG | 0.560570 | 5.3:-6172951 | MS.gene21536:CDS |
| ATCTATGCAGAATGTTGCTG+AGG | 0.563089 | 5.3:-6173177 | MS.gene21536:intron |
| TGCTAACCTGGAAGATAGAA+GGG | 0.563120 | 5.3:-6169421 | MS.gene21536:intron |
| GGCGCGTGGGTCCCACGCGA+GGG | 0.569797 | 5.3:-6176229 | MS.gene21536:CDS |
| AGTGAAGATGAAGTTGATGA+TGG | 0.571990 | 5.3:-6169447 | MS.gene21536:CDS |
| TATGCGGCGAGTGCGTTGAA+AGG | 0.572698 | 5.3:-6176497 | MS.gene21536:CDS |
| ATGAGGAAATGATGGGCGCG+TGG | 0.578257 | 5.3:-6176243 | MS.gene21536:CDS |
| TCTTCACCGGAATTATGCAC+CGG | 0.580245 | 5.3:+6179369 | None:intergenic |
| GTAGATATCCTGTCCAGCAA+AGG | 0.581784 | 5.3:-6172311 | MS.gene21536:intron |
| AATTTGCAGAATGAGGGAAA+TGG | 0.583836 | 5.3:-6172254 | MS.gene21536:CDS |
| TGCAGAATGAGGGAAATGGT+GGG | 0.583899 | 5.3:-6172250 | MS.gene21536:CDS |
| GGCATGGCAATTGAAGCACC+TGG | 0.583904 | 5.3:+6172158 | None:intergenic |
| GACAACCAACCAGAAGAAGA+AGG | 0.585205 | 5.3:+6173133 | None:intergenic |
| TACGCCGGCGTCGGAGACGG+CGG | 0.590335 | 5.3:-6176292 | MS.gene21536:CDS |
| ACGCGAGGGATTCAGGGTAG+AGG | 0.591518 | 5.3:-6176215 | MS.gene21536:intron |
| TGTGGTAGGGTACTGGCTGA+TGG | 0.593400 | 5.3:-6168637 | MS.gene21536:intron |
| GCAGAATGAGGGAAATGGTG+GGG | 0.599493 | 5.3:-6172249 | MS.gene21536:CDS |
| GGTTTCGACTACGCCGGCGT+CGG | 0.600309 | 5.3:-6176301 | MS.gene21536:CDS |
| GTTCACTGCTTGGTAATCAT+CGG | 0.601850 | 5.3:+6173809 | None:intergenic |
| CGACATCGGTGGCAGTGGAG+AGG | 0.601952 | 5.3:-6176592 | MS.gene21536:CDS |
| TCATGTTCCGTGGGCTTGTG+AGG | 0.609215 | 5.3:-6168614 | MS.gene21536:CDS |
| AATGTGCTTCGAAAGACTGA+AGG | 0.611487 | 5.3:-6177209 | MS.gene21536:intron |
| TCTATGCAGAATGTTGCTGA+GGG | 0.614958 | 5.3:-6173176 | MS.gene21536:intron |
| CAACTTCATCTTCACTATCA+CGG | 0.618030 | 5.3:+6169454 | None:intergenic |
| GGATCCGATGAGGAAATGAT+GGG | 0.621216 | 5.3:-6176250 | MS.gene21536:CDS |
| TCGGGAGCGGAGGTCGTATG+CGG | 0.621701 | 5.3:-6176513 | MS.gene21536:CDS |
| TCCGCGTAATCCATGGCACT+AGG | 0.623801 | 5.3:+6172206 | None:intergenic |
| TCCTAGTGCCATGGATTACG+CGG | 0.624999 | 5.3:-6172207 | MS.gene21536:CDS |
| TGCCATGCCCCAATCTTCTG+AGG | 0.626073 | 5.3:-6172144 | MS.gene21536:CDS |
| GTCCTCAGAAGATTGGGGCA+TGG | 0.626934 | 5.3:+6172142 | None:intergenic |
| TGCGTTGCCAGTGTGATCGA+CGG | 0.631852 | 5.3:-6176615 | MS.gene21536:CDS |
| CTACCCTGAATCCCTCGCGT+GGG | 0.632553 | 5.3:+6176218 | None:intergenic |
| ATCTGGAAAGTAACAACCGA+CGG | 0.632750 | 5.3:+6176666 | None:intergenic |
| TGAGGAAATGATGGGCGCGT+GGG | 0.633377 | 5.3:-6176242 | MS.gene21536:CDS |
| CGGCGGTGATGATGCGACGG+CGG | 0.640672 | 5.3:-6176275 | MS.gene21536:CDS |
| AGACGGCGGTGATGATGCGA+CGG | 0.642466 | 5.3:-6176278 | MS.gene21536:CDS |
| TCTACCCTGAATCCCTCGCG+TGG | 0.643548 | 5.3:+6176217 | None:intergenic |
| GATGTCGCCGTCGATCACAC+TGG | 0.645622 | 5.3:+6176608 | None:intergenic |
| AAGGTTGCGAGCAGGAATTG+TGG | 0.646352 | 5.3:-6177255 | MS.gene21536:CDS |
| TCTCGAAAGCGTAGACCGAG+AGG | 0.648729 | 5.3:-6173023 | MS.gene21536:CDS |
| GGAGATAGTGAATTCGTCGT+CGG | 0.651680 | 5.3:-6176567 | MS.gene21536:CDS |
| GAAAATGCCTCACAAGCCCA+CGG | 0.656006 | 5.3:+6168607 | None:intergenic |
| GTGATCGACGGCGACATCGG+TGG | 0.656569 | 5.3:-6176603 | MS.gene21536:CDS |
| TCAGGGTCCTCAGAAGATTG+GGG | 0.658194 | 5.3:+6172137 | None:intergenic |
| AACTGACGTATTGAGATCAG+AGG | 0.660479 | 5.3:-6173775 | MS.gene21536:intron |
| GACGGCGGAGGGATCCGATG+AGG | 0.667524 | 5.3:-6176260 | MS.gene21536:CDS |
| GATGAAGGTTTCGACTACGC+CGG | 0.668522 | 5.3:-6176307 | MS.gene21536:CDS |
| CATATGTAAACAAATTCCGC+CGG | 0.669136 | 5.3:+6176636 | None:intergenic |
| ATTCGTCGTCGGAGTCAGGG+AGG | 0.680972 | 5.3:-6176556 | MS.gene21536:CDS |
| TTTCCAGATGAAGTTTCCGG+CGG | 0.687107 | 5.3:-6176652 | MS.gene21536:CDS |
| TTGGAAAACTACCAAAACGG+GGG | 0.690298 | 5.3:-6168119 | MS.gene21536:CDS |
| AGATATCCTGTCCAGCAAAG+GGG | 0.705599 | 5.3:-6172309 | MS.gene21536:CDS |
| ATTGGAAAACTACCAAAACG+GGG | 0.706807 | 5.3:-6168120 | MS.gene21536:CDS |
| GGAGTTCTACAGAAAGACAA+CGG | 0.724077 | 5.3:-6172930 | MS.gene21536:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 6168097 | 6179409 | 6168097 | ID=MS.gene21536 |
| chr5.3 | mRNA | 6168097 | 6179409 | 6168097 | ID=MS.gene21536.t1;Parent=MS.gene21536 |
| chr5.3 | exon | 6179349 | 6179409 | 6179349 | ID=MS.gene21536.t1.exon1;Parent=MS.gene21536.t1 |
| chr5.3 | CDS | 6179349 | 6179409 | 6179349 | ID=cds.MS.gene21536.t1;Parent=MS.gene21536.t1 |
| chr5.3 | exon | 6177210 | 6177274 | 6177210 | ID=MS.gene21536.t1.exon2;Parent=MS.gene21536.t1 |
| chr5.3 | CDS | 6177210 | 6177274 | 6177210 | ID=cds.MS.gene21536.t1;Parent=MS.gene21536.t1 |
| chr5.3 | exon | 6176449 | 6176700 | 6176449 | ID=MS.gene21536.t1.exon3;Parent=MS.gene21536.t1 |
| chr5.3 | CDS | 6176449 | 6176700 | 6176449 | ID=cds.MS.gene21536.t1;Parent=MS.gene21536.t1 |
| chr5.3 | exon | 6176216 | 6176362 | 6176216 | ID=MS.gene21536.t1.exon4;Parent=MS.gene21536.t1 |
| chr5.3 | CDS | 6176216 | 6176362 | 6176216 | ID=cds.MS.gene21536.t1;Parent=MS.gene21536.t1 |
| chr5.3 | exon | 6173919 | 6173972 | 6173919 | ID=MS.gene21536.t1.exon5;Parent=MS.gene21536.t1 |
| chr5.3 | CDS | 6173919 | 6173972 | 6173919 | ID=cds.MS.gene21536.t1;Parent=MS.gene21536.t1 |
| chr5.3 | exon | 6173776 | 6173835 | 6173776 | ID=MS.gene21536.t1.exon6;Parent=MS.gene21536.t1 |
| chr5.3 | CDS | 6173776 | 6173835 | 6173776 | ID=cds.MS.gene21536.t1;Parent=MS.gene21536.t1 |
| chr5.3 | exon | 6173143 | 6173189 | 6173143 | ID=MS.gene21536.t1.exon7;Parent=MS.gene21536.t1 |
| chr5.3 | CDS | 6173143 | 6173189 | 6173143 | ID=cds.MS.gene21536.t1;Parent=MS.gene21536.t1 |
| chr5.3 | exon | 6172931 | 6173052 | 6172931 | ID=MS.gene21536.t1.exon8;Parent=MS.gene21536.t1 |
| chr5.3 | CDS | 6172931 | 6173052 | 6172931 | ID=cds.MS.gene21536.t1;Parent=MS.gene21536.t1 |
| chr5.3 | exon | 6172053 | 6172329 | 6172053 | ID=MS.gene21536.t1.exon9;Parent=MS.gene21536.t1 |
| chr5.3 | CDS | 6172053 | 6172329 | 6172053 | ID=cds.MS.gene21536.t1;Parent=MS.gene21536.t1 |
| chr5.3 | exon | 6170017 | 6170065 | 6170017 | ID=MS.gene21536.t1.exon10;Parent=MS.gene21536.t1 |
| chr5.3 | CDS | 6170017 | 6170065 | 6170017 | ID=cds.MS.gene21536.t1;Parent=MS.gene21536.t1 |
| chr5.3 | exon | 6169422 | 6169505 | 6169422 | ID=MS.gene21536.t1.exon11;Parent=MS.gene21536.t1 |
| chr5.3 | CDS | 6169422 | 6169505 | 6169422 | ID=cds.MS.gene21536.t1;Parent=MS.gene21536.t1 |
| chr5.3 | exon | 6168789 | 6168868 | 6168789 | ID=MS.gene21536.t1.exon12;Parent=MS.gene21536.t1 |
| chr5.3 | CDS | 6168789 | 6168868 | 6168789 | ID=cds.MS.gene21536.t1;Parent=MS.gene21536.t1 |
| chr5.3 | exon | 6168562 | 6168651 | 6168562 | ID=MS.gene21536.t1.exon13;Parent=MS.gene21536.t1 |
| chr5.3 | CDS | 6168562 | 6168651 | 6168562 | ID=cds.MS.gene21536.t1;Parent=MS.gene21536.t1 |
| chr5.3 | exon | 6168097 | 6168220 | 6168097 | ID=MS.gene21536.t1.exon14;Parent=MS.gene21536.t1 |
| chr5.3 | CDS | 6168097 | 6168220 | 6168097 | ID=cds.MS.gene21536.t1;Parent=MS.gene21536.t1 |
| Gene Sequence |
| Protein sequence |
