AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40157

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40157.t1 MTR_4g092040 95.041 1230 60 1 1 1229 1 1230 0.0 2314
MS.gene40157.t1 MTR_4g092040 94.742 1065 55 1 166 1229 1 1065 0.0 1984
MS.gene40157.t1 MTR_4g092040 27.559 381 202 12 154 496 337 681 7.97e-12 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40157.t1 AT1G61690 47.045 1286 508 37 1 1228 1 1171 0.0 906
MS.gene40157.t1 AT1G20110 44.262 61 31 2 16 73 450 510 6.61e-11 67.0

Find 261 sgRNAs with CRISPR-Local

Find 414 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTTGCGTCAAGAAATTT+TGG 0.135004 4.2:+20544697 MS.gene40157:CDS
CTTCTTTACGAGGGAATAAT+TGG 0.166408 4.2:+20540079 MS.gene40157:CDS
CAGTTGGGCTTCCAGATTCT+TGG 0.186211 4.2:-20544186 None:intergenic
GGCCATCTCTCCGTAGCTTT+AGG 0.215556 4.2:-20544979 None:intergenic
GCCATCTCTCCGTAGCTTTA+GGG 0.246953 4.2:-20544978 None:intergenic
TATCTTGGCTTCCAATGTTT+TGG 0.251942 4.2:-20543814 None:intergenic
TTTGTCAAAGGTTTGAGATT+TGG 0.253265 4.2:-20543019 None:intergenic
GTACTAGTAGCTTCCTTAAC+AGG 0.257183 4.2:-20544044 None:intergenic
TTGGTTAGGGTTCTCGATTT+TGG 0.275780 4.2:-20545227 None:intergenic
GAAATTCTCAACCAGATTCT+TGG 0.290064 4.2:+20542019 MS.gene40157:CDS
AATTCAATGCTTACTAATCT+AGG 0.296937 4.2:+20543978 MS.gene40157:CDS
AGCTGGTGCTTTGGAATCAC+TGG 0.301108 4.2:+20542969 MS.gene40157:CDS
CCCTCTCTTAAAAGCTTTCA+AGG 0.313296 4.2:-20542311 None:intergenic
GCCATTTCTGATAATCTTGA+TGG 0.313917 4.2:+20542904 MS.gene40157:CDS
CTGAATTCATTGTTAACATC+TGG 0.325263 4.2:+20545373 MS.gene40157:CDS
TGCTCATGATCATGCAGATT+TGG 0.328715 4.2:-20542856 None:intergenic
ATCCACCGCATCTGACTTAT+CGG 0.329738 4.2:-20545080 None:intergenic
CAATCGTCATCGACGAATAC+TGG 0.333635 4.2:+20543438 MS.gene40157:CDS
GATGCTCATCATGTGCAAAA+TGG 0.344797 4.2:-20544310 None:intergenic
CCCTGAGTTAGCTGGTGCTT+TGG 0.345146 4.2:+20542960 MS.gene40157:CDS
TCATCAACTGCCACATCAAC+TGG 0.345429 4.2:-20543906 None:intergenic
AAAGTCAGCAAACTTGAGTT+TGG 0.346435 4.2:+20542561 MS.gene40157:CDS
AACGGCTTTGCAGCAGCATT+CGG 0.347511 4.2:-20544899 None:intergenic
ACGGCTTTGCAGCAGCATTC+GGG 0.356227 4.2:-20544898 None:intergenic
AGCAATGGCGATATTGAAAA+AGG 0.361303 4.2:+20543116 MS.gene40157:CDS
TTGCGACAGGGACGCAGGGC+TGG 0.361700 4.2:+20541826 MS.gene40157:CDS
AGCGGGTCCTACTATTCCTT+TGG 0.366228 4.2:+20544532 MS.gene40157:CDS
AGAGCTGGGATGGTCTGATT+TGG 0.366288 4.2:+20542519 MS.gene40157:CDS
AACTGAAACAACGAATAGTA+TGG 0.379012 4.2:+20542186 MS.gene40157:CDS
AGCATCCATCTCTTCTATCT+TGG 0.379511 4.2:-20543829 None:intergenic
TCTTTCACTCCTTACGGACT+TGG 0.380081 4.2:+20543551 MS.gene40157:CDS
CATATGCCAGATCTACTTAC+TGG 0.381048 4.2:+20544431 MS.gene40157:CDS
TGCAGAGGAAGTCCTTAAAA+TGG 0.381959 4.2:+20543791 MS.gene40157:CDS
AGTTTGAGTTCTGAAATTAC+TGG 0.385842 4.2:+20543222 MS.gene40157:CDS
TGCAAAAGTTTCTGCAATCA+AGG 0.386217 4.2:-20542599 None:intergenic
GCACTCACCTCAGCAGATAA+TGG 0.391318 4.2:-20544332 None:intergenic
GTTCTTCCAGTTGCTTCTCT+AGG 0.393214 4.2:-20542750 None:intergenic
ATTGAGAAGGGAAGGGAAAA+TGG 0.395881 4.2:+20543341 MS.gene40157:CDS
TCAGCAACAAGTCCTTGTTT+TGG 0.397436 4.2:-20544223 None:intergenic
GGCTCTCAAAATCTTCCAGT+TGG 0.398361 4.2:-20544202 None:intergenic
GAAGAACAGGAACTCCTTGC+TGG 0.401130 4.2:+20542766 MS.gene40157:CDS
TGATGTGTTGGAAACATTGC+TGG 0.402289 4.2:-20544849 None:intergenic
CGTTGATGCCTCCAATTGCC+AGG 0.402559 4.2:+20540104 MS.gene40157:CDS
TGAGAGGCAAGCAGATGCTT+TGG 0.402822 4.2:+20542342 MS.gene40157:CDS
ACCATCAAGATTATCAGAAA+TGG 0.406595 4.2:-20542905 None:intergenic
CTCAGAAAAGTATCATCAAC+TGG 0.408151 4.2:-20543648 None:intergenic
CAAGTGAAAATCTCACAAAA+AGG 0.409098 4.2:+20544357 MS.gene40157:CDS
GCTCTCAAAATCTTCCAGTT+GGG 0.415509 4.2:-20544201 None:intergenic
TGCAAAGACAGGTGAAGAAA+AGG 0.415846 4.2:+20542618 MS.gene40157:CDS
ACATTTGACTGGTTTGTGTT+TGG 0.416861 4.2:-20544644 None:intergenic
AGAATTTCATCTTCATCTCT+AGG 0.417922 4.2:-20542004 None:intergenic
TTGCTGGTGGAGGCAGTGTT+TGG 0.418174 4.2:-20544833 None:intergenic
AGCGGAACAACCCTGGCAAT+TGG 0.421722 4.2:-20540115 None:intergenic
AGAGAAGCAACTGGAAGAAC+AGG 0.422268 4.2:+20542753 MS.gene40157:CDS
CAAGAATCTGGAAGCCCAAC+TGG 0.425253 4.2:+20544187 MS.gene40157:CDS
CTAGTACTGTCACAACTTCA+AGG 0.427339 4.2:+20544060 MS.gene40157:CDS
TTCCCTCGTAAAGAAGGCTT+TGG 0.427802 4.2:-20540072 None:intergenic
ACGCAAAGCCATTCTTTGTT+GGG 0.429998 4.2:-20541734 None:intergenic
AGTGACCGATGAGGATTTGA+TGG 0.430553 4.2:+20542936 MS.gene40157:CDS
TGCGACAGGGACGCAGGGCT+GGG 0.431204 4.2:+20541827 MS.gene40157:CDS
TACTTCAGAACTTCAAGAGC+TGG 0.434040 4.2:+20542504 MS.gene40157:CDS
CACGCAAAGCCATTCTTTGT+TGG 0.435300 4.2:-20541735 None:intergenic
AAGAATGGCTTTGCGTGGAC+AGG 0.438151 4.2:+20541741 MS.gene40157:CDS
TGTGCAAAATGGGAGTTTGC+AGG 0.440792 4.2:-20544299 None:intergenic
TGTTCCAGATTATGCTTCTC+AGG 0.442874 4.2:+20544667 MS.gene40157:CDS
TGAAGCGCAGTTGGAGGAGT+TGG 0.444540 4.2:+20545045 MS.gene40157:CDS
TGACCGTTTCAAGTTGCAAC+AGG 0.444966 4.2:+20544931 MS.gene40157:CDS
CAACCTCAGTCTTGTCAATC+CGG 0.445432 4.2:-20542645 None:intergenic
GGCCTGTCGAAGTTCGTCCC+TGG 0.446077 4.2:-20544771 None:intergenic
CAATTATTCCCTCGTAAAGA+AGG 0.446224 4.2:-20540078 None:intergenic
TTAGAGAAGAGGTTGGAGGA+TGG 0.449154 4.2:+20544800 MS.gene40157:CDS
GCGGTTAGTTTGAAACGATC+AGG 0.449589 4.2:+20545295 MS.gene40157:CDS
GCTCTCTTTGAATCATCAAC+CGG 0.451246 4.2:-20543290 None:intergenic
AGCATATCTTTCACTCCTTA+CGG 0.451437 4.2:+20543545 MS.gene40157:CDS
GTTGGAGGAGTTGGCTGCTC+AGG 0.452082 4.2:+20545054 MS.gene40157:CDS
CCATTACCAGTAAGTAGATC+TGG 0.452648 4.2:-20544437 None:intergenic
AAAGACATTTCTGTTGAGTC+TGG 0.453344 4.2:+20542433 MS.gene40157:CDS
TCGTCCTGAGAAGCATAATC+TGG 0.454913 4.2:-20544671 None:intergenic
TTGAACAACATTTGATGTGT+TGG 0.459609 4.2:-20544861 None:intergenic
AAAGGAAGGCACTTGCTTTG+AGG 0.464115 4.2:+20544120 MS.gene40157:CDS
TTATCTGCTCTGAAAGCTAC+TGG 0.464175 4.2:+20545139 MS.gene40157:CDS
GAGAACTCTTAGCGTTGAAA+AGG 0.464290 4.2:+20544102 MS.gene40157:CDS
GTAAGCATTGAATTCAATGC+TGG 0.472004 4.2:-20543969 None:intergenic
ATTGCTCATAGCTCCTCAAC+TGG 0.473098 4.2:+20544398 MS.gene40157:CDS
TCCCTTCCCGTCTTAAAGCA+AGG 0.473525 4.2:-20543758 None:intergenic
GTTACGTCAGCAAGCTTTAG+CGG 0.475113 4.2:+20542240 MS.gene40157:CDS
AATCTGGAACAACATTTGAC+TGG 0.475130 4.2:-20544655 None:intergenic
CCAGATCTACTTACTGGTAA+TGG 0.475511 4.2:+20544437 MS.gene40157:CDS
AAATTTCTTGACGCAAAGAA+AGG 0.475848 4.2:-20544694 None:intergenic
AGCGCGATTTGAGTTGCGAC+AGG 0.475901 4.2:+20541813 MS.gene40157:CDS
CAGAAGATGTTAGCGAAGAT+CGG 0.476728 4.2:+20540042 None:intergenic
AGCAGAAGCTGAGTTAGAAC+GGG 0.476768 4.2:+20545012 MS.gene40157:CDS
CAGCTTCTGCTTCCTCTGTT+CGG 0.477351 4.2:-20545000 None:intergenic
GTTAACCTTGCGATTGATGA+AGG 0.479727 4.2:-20540145 None:intergenic
CCCTTCCCGTCTTAAAGCAA+GGG 0.482007 4.2:-20543757 None:intergenic
GCTTGCTGACGTAACTCATC+AGG 0.482310 4.2:-20542232 None:intergenic
TCAGAGCAGATAAGAGTTGA+GGG 0.485201 4.2:-20545129 None:intergenic
ACTCCTGTTGCAACTTGAAA+CGG 0.485424 4.2:-20544934 None:intergenic
CATAAAAGAAGAACCTGTTA+AGG 0.489001 4.2:+20544031 MS.gene40157:CDS
GGATTGTTCGAAGATTTAGA+AGG 0.489464 4.2:-20543597 None:intergenic
GCATCTTCTTCAAGTACTAC+AGG 0.490304 4.2:+20542121 MS.gene40157:CDS
GGCACTTGCTTTGAGGCGCA+AGG 0.490504 4.2:+20544127 MS.gene40157:CDS
CCTCGTAAAGAAGGCTTTGG+TGG 0.490657 4.2:-20540069 None:intergenic
GCAAAGACAGGTGAAGAAAA+GGG 0.490919 4.2:+20542619 MS.gene40157:CDS
CAGGGACGCAGGGCTGGGAG+AGG 0.492123 4.2:+20541832 MS.gene40157:CDS
TGAATTCATTGTTAACATCT+GGG 0.492329 4.2:+20545374 MS.gene40157:CDS
GAGGAGTCCAAAGGAATAGT+AGG 0.493103 4.2:-20544539 None:intergenic
CTTTCACTCCTTACGGACTT+GGG 0.493296 4.2:+20543552 MS.gene40157:CDS
GCCTCTTCTAGCTTCTTACA+AGG 0.494213 4.2:-20541790 None:intergenic
AGGAAAACAAGCTGAAGCCT+TGG 0.495773 4.2:+20545315 MS.gene40157:CDS
AAACGGTCACGACTAGTAAA+CGG 0.495896 4.2:-20544917 None:intergenic
TTCTTTACGAGGGAATAATT+GGG 0.496857 4.2:+20540080 MS.gene40157:CDS
AGAAGGTTCTTGCATTGAGA+AGG 0.498127 4.2:+20543328 MS.gene40157:CDS
ATGCAGCGCTAACTGATCTC+TGG 0.498371 4.2:-20542102 None:intergenic
CACTCCTTACGGACTTGGGT+TGG 0.500597 4.2:+20543556 MS.gene40157:CDS
TAAGCTTGCAGAAGCTAAAG+AGG 0.501280 4.2:+20542708 MS.gene40157:CDS
GTGCTTTGGAATCACTGGGT+TGG 0.504177 4.2:+20542974 MS.gene40157:CDS
AGAAGATGTTAGCGAAGATC+GGG 0.504948 4.2:+20540043 None:intergenic
GAAGAATAATGTACAGGTTG+AGG 0.507140 4.2:+20543854 MS.gene40157:CDS
GGCAATTGAAGCGCAGTTGG+AGG 0.509228 4.2:+20545039 MS.gene40157:CDS
TGAGCATCAGCTGATGGAGA+TGG 0.510663 4.2:+20543401 MS.gene40157:CDS
CAACCTCTTCTCTAACAGCT+TGG 0.511169 4.2:-20544792 None:intergenic
GCCACTACGACCTTTGTCAC+TGG 0.511282 4.2:-20542080 None:intergenic
ATGCTCATCATGTGCAAAAT+GGG 0.511451 4.2:-20544309 None:intergenic
TATAAAATTCTGAAAGGAGA+CGG 0.512824 4.2:+20542274 MS.gene40157:CDS
TCCAAAGCACCAGCTAACTC+AGG 0.514575 4.2:-20542961 None:intergenic
AAGCAGAAGCTGAGTTAGAA+CGG 0.518122 4.2:+20545011 MS.gene40157:CDS
CAGAACTTCAAGAGCTGGGA+TGG 0.518671 4.2:+20542509 MS.gene40157:CDS
CCTTAAAATGGCCAAAACAT+TGG 0.518990 4.2:+20543803 MS.gene40157:CDS
TTGAGTTGCGACAGGGACGC+AGG 0.519807 4.2:+20541821 MS.gene40157:CDS
TAGCAACGCTTCTTTGTCAA+AGG 0.522640 4.2:-20543031 None:intergenic
AGCCAGGGACGAACTTCGAC+AGG 0.523706 4.2:+20544769 MS.gene40157:CDS
TTTAAGACGGGAAGGGAAAA+CGG 0.523794 4.2:+20543764 MS.gene40157:CDS
TCCTCAGACTGGTAAAGATA+AGG 0.524696 4.2:+20542474 MS.gene40157:CDS
AAGTTGTACCCAACAAAGAA+TGG 0.524769 4.2:+20541726 MS.gene40157:CDS
AGAGAGGGAAGGAGCTTGAG+AGG 0.525287 4.2:+20542326 MS.gene40157:CDS
GAAAGTGACTTAAACAACAC+TGG 0.526972 4.2:+20543150 MS.gene40157:CDS
TTGATGGACCCTGAGTTAGC+TGG 0.527871 4.2:+20542952 MS.gene40157:CDS
AAAGGCCCTTGCTTTAAGAC+GGG 0.530144 4.2:+20543752 MS.gene40157:CDS
GTCAGCAAACTTGAGTTTGG+AGG 0.531192 4.2:+20542564 MS.gene40157:CDS
GCTGGTGCTTTGGAATCACT+GGG 0.535449 4.2:+20542970 MS.gene40157:CDS
GGCACGTCGAAGAGCATCCA+AGG 0.535888 4.2:-20545332 None:intergenic
AAGGAAAACTAACAGAAGCC+AGG 0.536128 4.2:+20544753 MS.gene40157:CDS
TTCAGAGCAGATAAGAGTTG+AGG 0.537619 4.2:-20545130 None:intergenic
CAGCACCACTGTAGAAGGTG+TGG 0.537683 4.2:+20541688 MS.gene40157:intron
ATGTGGCAGTTGATGAAGAA+AGG 0.539322 4.2:+20543913 MS.gene40157:CDS
ATAAAATTCTGAAAGGAGAC+GGG 0.540491 4.2:+20542275 MS.gene40157:CDS
AAATAATAATGCCGCTTCCA+CGG 0.543317 4.2:+20543254 MS.gene40157:CDS
AAAGTAAAGCAGAAATTCAG+AGG 0.543735 4.2:+20543709 MS.gene40157:CDS
AACGTCAAGCCCTAAAGCTA+CGG 0.544391 4.2:+20544969 MS.gene40157:CDS
GAAAGGCCCTTGCTTTAAGA+CGG 0.545586 4.2:+20543751 MS.gene40157:CDS
ACTTCAGAACTTCAAGAGCT+GGG 0.547171 4.2:+20542505 MS.gene40157:CDS
GGGAGAAATAGAAGAGGCTG+AGG 0.548311 4.2:+20544148 MS.gene40157:CDS
TGAATTGAGCGGAACAACCC+TGG 0.551547 4.2:-20540122 None:intergenic
GAATAGTATGGCTGTTGATG+TGG 0.551719 4.2:+20542198 MS.gene40157:CDS
GAAGGTTCTTGCATTGAGAA+GGG 0.551906 4.2:+20543329 MS.gene40157:CDS
GACTTCTACTCGCTGCATGC+AGG 0.553919 4.2:-20543467 None:intergenic
GCCCTTGCTTTAAGACGGGA+AGG 0.554192 4.2:+20543756 MS.gene40157:CDS
CAAGGTTCACATATACGTAC+AGG 0.556070 4.2:-20541772 None:intergenic
GTAAGGAGTGAAAGATATGC+TGG 0.556679 4.2:-20543543 None:intergenic
GCAGTTGCTTTGAAAAGAGA+AGG 0.558654 4.2:+20544734 MS.gene40157:CDS
GTTCTTGCATTGAGAAGGGA+AGG 0.558660 4.2:+20543333 MS.gene40157:CDS
AGCTGAGTTAGAACGGGCAA+AGG 0.559624 4.2:+20545018 MS.gene40157:CDS
AATCTAGGATGGAAGGATGA+AGG 0.560283 4.2:+20543993 MS.gene40157:CDS
AAAGGCAATTGAAGCGCAGT+TGG 0.560739 4.2:+20545036 MS.gene40157:CDS
TTCAGTTAATTCATCTCAAG+CGG 0.560902 4.2:+20544514 MS.gene40157:CDS
CTTGTTGCTGAATGCATCCA+AGG 0.561407 4.2:+20544235 MS.gene40157:CDS
GCTTACTAATCTAGGATGGA+AGG 0.561754 4.2:+20543986 MS.gene40157:CDS
GCCCTAAAGCTACGGAGAGA+TGG 0.561928 4.2:+20544977 MS.gene40157:CDS
AGCACCACTGTAGAAGGTGT+GGG 0.564062 4.2:+20541689 MS.gene40157:intron
CTTCAATTCTCCAGTTGATG+TGG 0.564266 4.2:+20543896 MS.gene40157:CDS
CCACCAAAGCCTTCTTTACG+AGG 0.565763 4.2:+20540069 MS.gene40157:CDS
GGACATGGTTGTTTCAGAAG+AGG 0.567833 4.2:+20543935 MS.gene40157:CDS
TTCAGCTAAGGAAAGCGGCT+CGG 0.568308 4.2:+20542368 MS.gene40157:CDS
GGCTCTAACGTTGAAGCGTG+AGG 0.568601 4.2:+20542684 MS.gene40157:CDS
AGGGAAAACGGAGGATGCAG+AGG 0.569218 4.2:+20543776 MS.gene40157:CDS
CATTCCAACCCAAGTCCGTA+AGG 0.571820 4.2:-20543560 None:intergenic
TCAGCAAACTTGAGTTTGGA+GGG 0.572121 4.2:+20542565 MS.gene40157:CDS
ACAAAGTCACTTCCTCAGAC+TGG 0.572322 4.2:+20542463 MS.gene40157:CDS
CAGGTGAAGAAAAGGGCAGC+CGG 0.572523 4.2:+20542626 MS.gene40157:CDS
CCTCTAGTTGAGGAAGGAAG+TGG 0.572860 4.2:+20543489 MS.gene40157:CDS
ATGCATAGAGTGGCCAGTTG+AGG 0.573144 4.2:-20544411 None:intergenic
GGAAGCCAAGATAGAAGAGA+TGG 0.573762 4.2:+20543824 MS.gene40157:CDS
GGAACAACCCTGGCAATTGG+AGG 0.574373 4.2:-20540112 None:intergenic
CGGCTTTGCAGCAGCATTCG+GGG 0.576851 4.2:-20544897 None:intergenic
TGCTCTGAAGAATAATGTAC+AGG 0.578058 4.2:+20543848 MS.gene40157:CDS
ATCAGCATTAATACGCGGCA+TGG 0.578083 4.2:+20542813 MS.gene40157:CDS
ATTCACCTTCATCAATCGCA+AGG 0.579513 4.2:+20540140 MS.gene40157:CDS
ATGCTTTGGAAATTCAGCTA+AGG 0.580873 4.2:+20542356 MS.gene40157:CDS
TTATCATCATATGTCTTCGA+AGG 0.581242 4.2:-20543618 None:intergenic
AGAATGGCTTTGCGTGGACA+GGG 0.583680 4.2:+20541742 MS.gene40157:CDS
CAATGCTTACTAATCTAGGA+TGG 0.583962 4.2:+20543982 MS.gene40157:CDS
GCTCTTAATCAAAAGCGAGC+AGG 0.584439 4.2:+20543081 MS.gene40157:CDS
TAAGCATTGAATTCAATGCT+GGG 0.587214 4.2:-20543968 None:intergenic
TGCCGATAAGTCAGATGCGG+TGG 0.587474 4.2:+20545078 MS.gene40157:CDS
AGTAAGTATAAAATTCTGAA+AGG 0.588864 4.2:+20542268 MS.gene40157:CDS
AGGCCAAGCTGTTAGAGAAG+AGG 0.593828 4.2:+20544789 MS.gene40157:CDS
AGAAGTCCTCTAGTTGAGGA+AGG 0.594048 4.2:+20543483 MS.gene40157:CDS
TCCTTATCTTTACCAGTCTG+AGG 0.594057 4.2:-20542475 None:intergenic
CACCAAAGCCTTCTTTACGA+GGG 0.594410 4.2:+20540070 MS.gene40157:CDS
GCTGTTAGAGAAGAGGTTGG+AGG 0.596896 4.2:+20544796 MS.gene40157:CDS
GTTGATGCCTCCAATTGCCA+GGG 0.598442 4.2:+20540105 MS.gene40157:CDS
GAGTAGAAGTCCTCTAGTTG+AGG 0.600375 4.2:+20543479 MS.gene40157:CDS
GGAAATTCAGCTAAGGAAAG+CGG 0.600558 4.2:+20542363 MS.gene40157:CDS
TCAGTTAATTCATCTCAAGC+GGG 0.600749 4.2:+20544515 MS.gene40157:CDS
AGTTCTTGAGCATCAGCTGA+TGG 0.601408 4.2:+20543395 MS.gene40157:CDS
TGAGTTGCGACAGGGACGCA+GGG 0.605256 4.2:+20541822 MS.gene40157:CDS
GTTCCGTCTGAATCATCTCA+CGG 0.608299 4.2:+20544269 MS.gene40157:CDS
AGGTAATACTGAAGAAGCAA+TGG 0.609705 4.2:+20543101 MS.gene40157:CDS
GCACTTGCTTTGAGGCGCAA+GGG 0.610522 4.2:+20544128 MS.gene40157:CDS
CAAGCTGTTAGAGAAGAGGT+TGG 0.612824 4.2:+20544793 MS.gene40157:CDS
AGGAAAACTAACAGAAGCCA+GGG 0.615723 4.2:+20544754 MS.gene40157:CDS
AAGCAATCAACCAGTGACAA+AGG 0.616395 4.2:+20542070 MS.gene40157:CDS
GTTGGAAACATTGCTGGTGG+AGG 0.616993 4.2:-20544843 None:intergenic
TGTGGCAGTTGATGAAGAAA+GGG 0.617619 4.2:+20543914 MS.gene40157:CDS
CAACAAAGAATGGCTTTGCG+TGG 0.621792 4.2:+20541736 MS.gene40157:CDS
GTCACAACTTCAAGGAACAA+AGG 0.622653 4.2:+20544068 MS.gene40157:CDS
AAAGTGACTTAAACAACACT+GGG 0.624537 4.2:+20543151 MS.gene40157:CDS
ACGTAACTCATCAGGAGTGG+TGG 0.624698 4.2:-20542224 None:intergenic
GCACCACTGTAGAAGGTGTG+GGG 0.625379 4.2:+20541690 MS.gene40157:intron
GCTCTAACGTTGAAGCGTGA+GGG 0.625690 4.2:+20542685 MS.gene40157:CDS
AGGGTCCATCAAATCCTCAT+CGG 0.627532 4.2:-20542941 None:intergenic
CCACTTCCTTCCTCAACTAG+AGG 0.628017 4.2:-20543489 None:intergenic
ACGGAGAGATGGCCGAACAG+AGG 0.628540 4.2:+20544988 MS.gene40157:CDS
TTTCCGTGAGATGATTCAGA+CGG 0.629170 4.2:-20544272 None:intergenic
AATCTGCATGATCATGAGCA+TGG 0.631390 4.2:+20542859 MS.gene40157:CDS
CAGAATCTTCAGCACCAGCA+AGG 0.632957 4.2:-20542780 None:intergenic
ACTCTTAGCGTTGAAAAGGA+AGG 0.636692 4.2:+20544106 MS.gene40157:CDS
ACCTTGTAAGAAGCTAGAAG+AGG 0.636754 4.2:+20541789 MS.gene40157:CDS
GTTGCCTTCTGGAAACATGT+CGG 0.637060 4.2:+20542399 MS.gene40157:CDS
GAAAGGAGACGGGAAATCTG+AGG 0.642954 4.2:+20542285 MS.gene40157:CDS
CCAAAGCACCAGCTAACTCA+GGG 0.645234 4.2:-20542960 None:intergenic
ACCAGTGACAAAGGTCGTAG+TGG 0.645600 4.2:+20542079 MS.gene40157:CDS
AATTCAGTCATTGAAAAGAG+AGG 0.645693 4.2:+20543059 MS.gene40157:CDS
CCCTTGCTTTAAGACGGGAA+GGG 0.647794 4.2:+20543757 MS.gene40157:CDS
AATAGTATGGCTGTTGATGT+GGG 0.649232 4.2:+20542199 MS.gene40157:CDS
CACCACTGTAGAAGGTGTGG+GGG 0.651612 4.2:+20541691 MS.gene40157:CDS
GCGCGATTTGAGTTGCGACA+GGG 0.652062 4.2:+20541814 MS.gene40157:CDS
AGATTCACTGAGGAGTCCAA+AGG 0.654296 4.2:-20544548 None:intergenic
AAGTAAAGCAGAAATTCAGA+GGG 0.660938 4.2:+20543710 MS.gene40157:CDS
GATCTGGCATATGCATAGAG+TGG 0.663140 4.2:-20544421 None:intergenic
GGTGGATGATGTGAGTGTTG+AGG 0.663208 4.2:+20545096 MS.gene40157:CDS
TTCGTCGATGACGATTGACT+CGG 0.671976 4.2:-20543432 None:intergenic
CTGACGTAACTCATCAGGAG+TGG 0.672100 4.2:-20542227 None:intergenic
TTCTTGCATTGAGAAGGGAA+GGG 0.677335 4.2:+20543334 MS.gene40157:CDS
ATATCCGACATGTTTCCAGA+AGG 0.678237 4.2:-20542403 None:intergenic
CCAGATTCTTGGTCAAAACG+AGG 0.680619 4.2:+20542030 MS.gene40157:CDS
GCGCAAGGGAGAAATAGAAG+AGG 0.680799 4.2:+20544142 MS.gene40157:CDS
TGTGTTGGAAACATTGCTGG+TGG 0.682302 4.2:-20544846 None:intergenic
GGATGCCGATAAGTCAGATG+CGG 0.687597 4.2:+20545075 MS.gene40157:CDS
AAAAGTTTCTGCAATCAAGG+AGG 0.697378 4.2:-20542596 None:intergenic
TAATTTAGAAGTGACCGATG+AGG 0.698604 4.2:+20542927 MS.gene40157:CDS
ATGAAGCAGAAGAAGAAATG+CGG 0.709038 4.2:+20543364 MS.gene40157:CDS
GTCTTGGCTGAGATTCACTG+AGG 0.710997 4.2:-20544558 None:intergenic
AGTTGATGAAGAAAGGGACA+TGG 0.716294 4.2:+20543920 MS.gene40157:CDS
TGAGCATCCATTATCTGCTG+AGG 0.716468 4.2:+20544325 MS.gene40157:CDS
CAGCCGGATTGACAAGACTG+AGG 0.720198 4.2:+20542642 MS.gene40157:CDS
ATTGATGAAGGTGAATTGAG+CGG 0.728556 4.2:-20540133 None:intergenic
GAGCTATCAGCATTAATACG+CGG 0.732772 4.2:+20542808 MS.gene40157:CDS
AAGACGGGAAGGGAAAACGG+AGG 0.744098 4.2:+20543767 MS.gene40157:CDS
TAATAATGCCGCTTCCACGG+TGG 0.746076 4.2:+20543257 MS.gene40157:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATATTGTAAAATTAGTT+AGG + chr4.2:20541292-20541311 MS.gene40157:intron 10.0%
!!! AACTAATTTTACAATATTTA+AGG - chr4.2:20541292-20541311 None:intergenic 10.0%
!! CAAAATTCCAAAATTTAATT+GGG - chr4.2:20541656-20541675 None:intergenic 15.0%
!! TCAAAATTCCAAAATTTAAT+TGG - chr4.2:20541657-20541676 None:intergenic 15.0%
!! TTGATAAAACAATTAAAACT+GGG - chr4.2:20541480-20541499 None:intergenic 15.0%
!! TTTGATAAAACAATTAAAAC+TGG - chr4.2:20541481-20541500 None:intergenic 15.0%
!! AAAGAACTATAAAGAGAAAA+TGG - chr4.2:20540870-20540889 None:intergenic 20.0%
!! AAGTGAAAATTGTATAGATT+TGG - chr4.2:20541338-20541357 None:intergenic 20.0%
!! AATTAAAGAAGAAAAACTGA+AGG + chr4.2:20545273-20545292 MS.gene40157:CDS 20.0%
!! ACTTATGTAATGAAGAAAAT+AGG - chr4.2:20541143-20541162 None:intergenic 20.0%
!! AGTAAGTATAAAATTCTGAA+AGG + chr4.2:20542268-20542287 MS.gene40157:CDS 20.0%
!! GTTAATTTCATGATTCTATA+TGG + chr4.2:20541549-20541568 MS.gene40157:intron 20.0%
!! TAAATGATAGAGAACAATAT+AGG + chr4.2:20540309-20540328 MS.gene40157:intron 20.0%
!!! ACAAAATAGGCTTTTAAATA+AGG - chr4.2:20540939-20540958 None:intergenic 20.0%
!!! TCTCTTCATTTAATTTTCTA+TGG + chr4.2:20541234-20541253 MS.gene40157:intron 20.0%
!!! TTCATTACATAAGTTTTGTT+TGG + chr4.2:20541149-20541168 MS.gene40157:intron 20.0%
!!! TTTTGATTTTGATAATCTCT+TGG + chr4.2:20542882-20542901 MS.gene40157:CDS 20.0%
! AAATGACAAGTTGTTAAACA+TGG - chr4.2:20540501-20540520 None:intergenic 25.0%
! AATTACTCTTGTTAATGCAA+CGG - chr4.2:20540423-20540442 None:intergenic 25.0%
! AATTCAATGCTTACTAATCT+AGG + chr4.2:20543978-20543997 MS.gene40157:CDS 25.0%
! ATAAAAAGTGAATTGTCTCT+AGG - chr4.2:20540603-20540622 None:intergenic 25.0%
! CAAAAAGAAGAATATAAAGC+AGG + chr4.2:20544491-20544510 MS.gene40157:CDS 25.0%
! CAGATTATAATAAGCAAACA+TGG - chr4.2:20541608-20541627 None:intergenic 25.0%
! CATTGAATAACTAATGTATC+TGG + chr4.2:20540626-20540645 MS.gene40157:intron 25.0%
! GAAAATTAAATGAAGAGAAG+AGG - chr4.2:20541232-20541251 None:intergenic 25.0%
! TAAAAAGTGAATTGTCTCTA+GGG - chr4.2:20540602-20540621 None:intergenic 25.0%
! TAAAGACCTTATCACAAAAT+AGG - chr4.2:20540952-20540971 None:intergenic 25.0%
! TGAAAATTGTATAGATTTGG+TGG - chr4.2:20541335-20541354 None:intergenic 25.0%
! TGAATTCATTGTTAACATCT+GGG + chr4.2:20545374-20545393 MS.gene40157:CDS 25.0%
!! GATTTGGAAAATGTATTTTC+AGG - chr4.2:20543006-20543025 None:intergenic 25.0%
!! TAAAAGCCTATTTTGTGATA+AGG + chr4.2:20540943-20540962 MS.gene40157:intron 25.0%
!! TAGGCTTTTAAATAAGGTTA+AGG - chr4.2:20540933-20540952 None:intergenic 25.0%
!! TATAAAATTCTGAAAGGAGA+CGG + chr4.2:20542274-20542293 MS.gene40157:CDS 25.0%
!! TATACCTTTAGTTTAACCAA+AGG - chr4.2:20541521-20541540 None:intergenic 25.0%
!! TTGTTAGGTTTAAAGGTTTT+TGG + chr4.2:20541065-20541084 MS.gene40157:intron 25.0%
!!! AAGAAATTTTGGCTCATAAA+AGG + chr4.2:20544708-20544727 MS.gene40157:CDS 25.0%
!!! ATGGTTTTAACAAAAATGAG+GGG - chr4.2:20541862-20541881 None:intergenic 25.0%
!!! ATTTCTGCTTTACTTTTTCT+TGG - chr4.2:20543705-20543724 None:intergenic 25.0%
!!! CATGGTTTTAACAAAAATGA+GGG - chr4.2:20541863-20541882 None:intergenic 25.0%
!!! GAATGTTGTTTTTGTATATC+TGG - chr4.2:20544583-20544602 None:intergenic 25.0%
!!! GTTAGTTTTGTTAGGTTTAA+AGG + chr4.2:20541058-20541077 MS.gene40157:intron 25.0%
!!! TCATGGTTTTAACAAAAATG+AGG - chr4.2:20541864-20541883 None:intergenic 25.0%
AAAGTAAAGCAGAAATTCAG+AGG + chr4.2:20543709-20543728 MS.gene40157:CDS 30.0%
AAAGTGACTTAAACAACACT+GGG + chr4.2:20543151-20543170 MS.gene40157:CDS 30.0%
AAATTTCTTGACGCAAAGAA+AGG - chr4.2:20544697-20544716 None:intergenic 30.0%
AACTGAAACAACGAATAGTA+TGG + chr4.2:20542186-20542205 MS.gene40157:CDS 30.0%
AAGTAAAGCAGAAATTCAGA+GGG + chr4.2:20543710-20543729 MS.gene40157:CDS 30.0%
AATTCAGTCATTGAAAAGAG+AGG + chr4.2:20543059-20543078 MS.gene40157:CDS 30.0%
ACCATCAAGATTATCAGAAA+TGG - chr4.2:20542908-20542927 None:intergenic 30.0%
ACTCTTGAGCTTAAAAAGAA+AGG + chr4.2:20543734-20543753 MS.gene40157:CDS 30.0%
AGAACCTTTGGTTAAACTAA+AGG + chr4.2:20541514-20541533 MS.gene40157:intron 30.0%
AGAATTTCATCTTCATCTCT+AGG - chr4.2:20542007-20542026 None:intergenic 30.0%
AGAGTGAATATTCAAAAGCA+TGG - chr4.2:20541956-20541975 None:intergenic 30.0%
AGTCACTGAAATTCAAATCA+TGG - chr4.2:20540248-20540267 None:intergenic 30.0%
ATAGTGAGTTATCCAATCTA+AGG - chr4.2:20541444-20541463 None:intergenic 30.0%
ATATTCACTCTAATTTCTCC+AGG + chr4.2:20541965-20541984 MS.gene40157:intron 30.0%
CAAAAGTCTGTTTAGCTAAA+AGG - chr4.2:20541108-20541127 None:intergenic 30.0%
CAAGTGAAAATCTCACAAAA+AGG + chr4.2:20544357-20544376 MS.gene40157:CDS 30.0%
CAATGTAATTCATCGGTATA+TGG - chr4.2:20540386-20540405 None:intergenic 30.0%
CCAAAATTTAATTGGGACAA+AGG - chr4.2:20541649-20541668 None:intergenic 30.0%
CTGAATTCATTGTTAACATC+TGG + chr4.2:20545373-20545392 MS.gene40157:CDS 30.0%
GGAAGCTAAACATACAATTA+GGG - chr4.2:20541422-20541441 None:intergenic 30.0%
GTAATGAAGAAAATAGGTCA+AGG - chr4.2:20541137-20541156 None:intergenic 30.0%
TAAAGAAGAAAAACTGAAGG+CGG + chr4.2:20545276-20545295 MS.gene40157:CDS 30.0%
TAGGTTTGAGTACATTAGTT+TGG + chr4.2:20541174-20541193 MS.gene40157:intron 30.0%
TAGTGAGTTATCCAATCTAA+GGG - chr4.2:20541443-20541462 None:intergenic 30.0%
TATATGGAAAACGAGATCTT+GGG + chr4.2:20541565-20541584 MS.gene40157:intron 30.0%
TATCAACAAGTAAGCTCATA+GGG + chr4.2:20541016-20541035 MS.gene40157:intron 30.0%
TGAAACACAAACAATATAGC+CGG - chr4.2:20540747-20540766 None:intergenic 30.0%
TTAGCAGCAATGTAATTCAT+CGG - chr4.2:20540393-20540412 None:intergenic 30.0%
TTATCATCATATGTCTTCGA+AGG - chr4.2:20543621-20543640 None:intergenic 30.0%
TTCAGTTAATTCATCTCAAG+CGG + chr4.2:20544514-20544533 MS.gene40157:CDS 30.0%
TTCTTTACGAGGGAATAATT+GGG + chr4.2:20540080-20540099 MS.gene40157:CDS 30.0%
TTGAACAACATTTGATGTGT+TGG - chr4.2:20544864-20544883 None:intergenic 30.0%
TTTAAAAAAGGCCAAGAATC+TGG + chr4.2:20544175-20544194 MS.gene40157:CDS 30.0%
TTTAAGCTTCATAGAACCTT+TGG + chr4.2:20541502-20541521 MS.gene40157:intron 30.0%
TTTAATTGGGACAAAGGATT+TGG - chr4.2:20541643-20541662 None:intergenic 30.0%
! AATTTTGCTTGTTTCATCGT+AGG + chr4.2:20540546-20540565 MS.gene40157:intron 30.0%
! AGTCACTTCTTTTCTTCTTT+GGG - chr4.2:20544609-20544628 None:intergenic 30.0%
! ATAAAATTCTGAAAGGAGAC+GGG + chr4.2:20542275-20542294 MS.gene40157:CDS 30.0%
! CAGCTTCTTTTCATACATTT+TGG - chr4.2:20545356-20545375 None:intergenic 30.0%
! CCTTTGTCCCAATTAAATTT+TGG + chr4.2:20541646-20541665 MS.gene40157:intron 30.0%
! GCTTTTGAATTCGAAAAAGA+AGG + chr4.2:20543311-20543330 MS.gene40157:CDS 30.0%
! TACTACAGGATTTTCTATTC+AGG + chr4.2:20542135-20542154 MS.gene40157:CDS 30.0%
! TTCTTTGCGTCAAGAAATTT+TGG + chr4.2:20544697-20544716 MS.gene40157:CDS 30.0%
! TTGAGTACATTAGTTTGGTT+TGG + chr4.2:20541179-20541198 MS.gene40157:intron 30.0%
! TTTGTCAAAGGTTTGAGATT+TGG - chr4.2:20543022-20543041 None:intergenic 30.0%
!! AGTTTGAGTTCTGAAATTAC+TGG + chr4.2:20543222-20543241 MS.gene40157:CDS 30.0%
!! GGCTGAGGAAATTTTAAAAA+AGG + chr4.2:20544163-20544182 MS.gene40157:CDS 30.0%
!! TAAGCATTGAATTCAATGCT+GGG - chr4.2:20543971-20543990 None:intergenic 30.0%
!! TAGGTTTAAAGGTTTTTGGA+TGG + chr4.2:20541069-20541088 MS.gene40157:intron 30.0%
!! TGTTTTTGTATATCTGGTCT+TGG - chr4.2:20544577-20544596 None:intergenic 30.0%
!!! AATTTTGGCTCATAAAAGGA+AGG + chr4.2:20544712-20544731 MS.gene40157:CDS 30.0%
!!! ACATAAGTTTTGTTTGGTCT+AGG + chr4.2:20541155-20541174 MS.gene40157:intron 30.0%
!!! TGGTTTTAACAAAAATGAGG+GGG - chr4.2:20541861-20541880 None:intergenic 30.0%
!!! TTGAGTAGGTTAGTTTTGTT+AGG + chr4.2:20541050-20541069 MS.gene40157:intron 30.0%
AAAAATCCTAGAGAAGCAAC+TGG + chr4.2:20542744-20542763 MS.gene40157:CDS 35.0%
AAAAGTTTCTGCAATCAAGG+AGG - chr4.2:20542599-20542618 None:intergenic 35.0%
AAAGACATTTCTGTTGAGTC+TGG + chr4.2:20542433-20542452 MS.gene40157:CDS 35.0%
AAAGTCAGCAAACTTGAGTT+TGG + chr4.2:20542561-20542580 MS.gene40157:CDS 35.0%
AAGTTGTACCCAACAAAGAA+TGG + chr4.2:20541726-20541745 MS.gene40157:CDS 35.0%
AATAGTATGGCTGTTGATGT+GGG + chr4.2:20542199-20542218 MS.gene40157:CDS 35.0%
AGATTAGCACAATCGAAGAA+TGG - chr4.2:20540174-20540193 None:intergenic 35.0%
AGCAATGGCGATATTGAAAA+AGG + chr4.2:20543116-20543135 MS.gene40157:CDS 35.0%
AGCATATCTTTCACTCCTTA+CGG + chr4.2:20543545-20543564 MS.gene40157:CDS 35.0%
AGGTAATACTGAAGAAGCAA+TGG + chr4.2:20543101-20543120 MS.gene40157:CDS 35.0%
AGGTCTATAGATAAGTGATG+AGG + chr4.2:20540992-20541011 MS.gene40157:intron 35.0%
AGGTCTTTAGATAAGTGATG+AGG + chr4.2:20540963-20540982 MS.gene40157:intron 35.0%
ATGAAGCAGAAGAAGAAATG+CGG + chr4.2:20543364-20543383 MS.gene40157:CDS 35.0%
ATGCTCATCATGTGCAAAAT+GGG - chr4.2:20544312-20544331 None:intergenic 35.0%
ATTAGCACAATCGAAGAATG+GGG - chr4.2:20540172-20540191 None:intergenic 35.0%
ATTGATGAAGGTGAATTGAG+CGG - chr4.2:20540136-20540155 None:intergenic 35.0%
CAATGCTTACTAATCTAGGA+TGG + chr4.2:20543982-20544001 MS.gene40157:CDS 35.0%
CAATTATTCCCTCGTAAAGA+AGG - chr4.2:20540081-20540100 None:intergenic 35.0%
CCTTAAAATGGCCAAAACAT+TGG + chr4.2:20543803-20543822 MS.gene40157:CDS 35.0%
CTATATGGAAAACGAGATCT+TGG + chr4.2:20541564-20541583 MS.gene40157:intron 35.0%
CTATCAACAAGTAAGCTCAT+AGG + chr4.2:20541015-20541034 MS.gene40157:intron 35.0%
CTTCTTTACGAGGGAATAAT+TGG + chr4.2:20540079-20540098 MS.gene40157:CDS 35.0%
GAAACACAAACAATATAGCC+GGG - chr4.2:20540746-20540765 None:intergenic 35.0%
GAAAGTGACTTAAACAACAC+TGG + chr4.2:20543150-20543169 MS.gene40157:CDS 35.0%
GAAATTCTCAACCAGATTCT+TGG + chr4.2:20542019-20542038 MS.gene40157:CDS 35.0%
GAAGAATAATGTACAGGTTG+AGG + chr4.2:20543854-20543873 MS.gene40157:CDS 35.0%
GATTAGCACAATCGAAGAAT+GGG - chr4.2:20540173-20540192 None:intergenic 35.0%
GCCATTTCTGATAATCTTGA+TGG + chr4.2:20542904-20542923 MS.gene40157:CDS 35.0%
GCTAAACATACAATTAGGGA+CGG - chr4.2:20541418-20541437 None:intergenic 35.0%
GGATTGTTCGAAGATTTAGA+AGG - chr4.2:20543600-20543619 None:intergenic 35.0%
GGGAAGCTAAACATACAATT+AGG - chr4.2:20541423-20541442 None:intergenic 35.0%
GGTTGTTGCTATGAAAAAGA+AGG + chr4.2:20542663-20542682 MS.gene40157:CDS 35.0%
GTGAATTGTCTCCTATAGTA+AGG + chr4.2:20540669-20540688 MS.gene40157:intron 35.0%
TAATTTAGAAGTGACCGATG+AGG + chr4.2:20542927-20542946 MS.gene40157:CDS 35.0%
TCAGTTAATTCATCTCAAGC+GGG + chr4.2:20544515-20544534 MS.gene40157:CDS 35.0%
TGCAAAAAGTTACTCCTGTT+GGG + chr4.2:20543196-20543215 MS.gene40157:CDS 35.0%
TGCAAAAGTTTCTGCAATCA+AGG - chr4.2:20542602-20542621 None:intergenic 35.0%
TTGCAAAAAGTTACTCCTGT+TGG + chr4.2:20543195-20543214 MS.gene40157:CDS 35.0%
! AAATAATAATGCCGCTTCCA+CGG + chr4.2:20543254-20543273 MS.gene40157:CDS 35.0%
! AACGAGGAACTTCTTTTATC+AGG + chr4.2:20542046-20542065 MS.gene40157:CDS 35.0%
! AATCTGGAACAACATTTGAC+TGG - chr4.2:20544658-20544677 None:intergenic 35.0%
! AGCTGAATTTTCTCTTGGTT+AGG - chr4.2:20545244-20545263 None:intergenic 35.0%
! AGGTTCTTCTTTTATGGTCA+CGG - chr4.2:20544027-20544046 None:intergenic 35.0%
! ATGTTTAGCTTCCCTTAGAT+TGG + chr4.2:20541429-20541448 MS.gene40157:intron 35.0%
! CAGTCACTTCTTTTCTTCTT+TGG - chr4.2:20544610-20544629 None:intergenic 35.0%
! GAGAATCAATTTTTCATGCC+CGG + chr4.2:20540725-20540744 MS.gene40157:intron 35.0%
! GCTGAATTTTCTCTTGGTTA+GGG - chr4.2:20545243-20545262 None:intergenic 35.0%
! GTAACAGAGTCTTTGATGAT+TGG - chr4.2:20540218-20540237 None:intergenic 35.0%
! TATCTTGGCTTCCAATGTTT+TGG - chr4.2:20543817-20543836 None:intergenic 35.0%
!! AACCAAGCTTTGAAAAAAGC+TGG - chr4.2:20540907-20540926 None:intergenic 35.0%
!! AAGGTTTTTGGATGGTCTAA+AGG + chr4.2:20541077-20541096 MS.gene40157:intron 35.0%
!! ACATTTGACTGGTTTGTGTT+TGG - chr4.2:20544647-20544666 None:intergenic 35.0%
!! ACCAAGCTTTGAAAAAAGCT+GGG - chr4.2:20540906-20540925 None:intergenic 35.0%
!! AGATTTTGAGAGCCAAAACA+AGG + chr4.2:20544211-20544230 MS.gene40157:CDS 35.0%
!! ATGCTTTGGAAATTCAGCTA+AGG + chr4.2:20542356-20542375 MS.gene40157:CDS 35.0%
!! CCTTGAAAGCTTTTAAGAGA+GGG + chr4.2:20542311-20542330 MS.gene40157:CDS 35.0%
!! CTCAGAAAAGTATCATCAAC+TGG - chr4.2:20543651-20543670 None:intergenic 35.0%
!! CTTCTAGCTGAATTTTCTCT+TGG - chr4.2:20545249-20545268 None:intergenic 35.0%
!! GTAAGCATTGAATTCAATGC+TGG - chr4.2:20543972-20543991 None:intergenic 35.0%
!! TGCTCTGAAGAATAATGTAC+AGG + chr4.2:20543848-20543867 MS.gene40157:CDS 35.0%
!!! CCAATGTTTTGGCCATTTTA+AGG - chr4.2:20543806-20543825 None:intergenic 35.0%
!!! CGGAACAGATTTTTTATCCT+TGG - chr4.2:20544255-20544274 None:intergenic 35.0%
!!! GCTTTTTTCAAAGCTTGGTT+TGG + chr4.2:20540907-20540926 MS.gene40157:intron 35.0%
!!! TCACAGTCTCTTGTTTTTCT+GGG - chr4.2:20545198-20545217 None:intergenic 35.0%
!!! TTCACAGTCTCTTGTTTTTC+TGG - chr4.2:20545199-20545218 None:intergenic 35.0%
AAACGGTCACGACTAGTAAA+CGG - chr4.2:20544920-20544939 None:intergenic 40.0%
AAGCAATCAACCAGTGACAA+AGG + chr4.2:20542070-20542089 MS.gene40157:CDS 40.0%
AAGCAGAAGCTGAGTTAGAA+CGG + chr4.2:20545011-20545030 MS.gene40157:CDS 40.0%
AAGGAAAACTAACAGAAGCC+AGG + chr4.2:20544753-20544772 MS.gene40157:CDS 40.0%
AATCTGCATGATCATGAGCA+TGG + chr4.2:20542859-20542878 MS.gene40157:CDS 40.0%
ACCTTATGGCTTACTCTTTC+AGG - chr4.2:20541371-20541390 None:intergenic 40.0%
ACCTTGTAAGAAGCTAGAAG+AGG + chr4.2:20541789-20541808 MS.gene40157:CDS 40.0%
ACGCAAAGCCATTCTTTGTT+GGG - chr4.2:20541737-20541756 None:intergenic 40.0%
ACTCCTGTTGCAACTTGAAA+CGG - chr4.2:20544937-20544956 None:intergenic 40.0%
ACTTCAGAACTTCAAGAGCT+GGG + chr4.2:20542505-20542524 MS.gene40157:CDS 40.0%
AGAAGGTTCTTGCATTGAGA+AGG + chr4.2:20543328-20543347 MS.gene40157:CDS 40.0%
AGCATCCATCTCTTCTATCT+TGG - chr4.2:20543832-20543851 None:intergenic 40.0%
AGGAAAACTAACAGAAGCCA+GGG + chr4.2:20544754-20544773 MS.gene40157:CDS 40.0%
AGTAAGCCATAAGGTCATTG+AGG + chr4.2:20541376-20541395 MS.gene40157:intron 40.0%
ATATCCGACATGTTTCCAGA+AGG - chr4.2:20542406-20542425 None:intergenic 40.0%
ATTCACCTTCATCAATCGCA+AGG + chr4.2:20540140-20540159 MS.gene40157:CDS 40.0%
ATTGAGAAGGGAAGGGAAAA+TGG + chr4.2:20543341-20543360 MS.gene40157:CDS 40.0%
CAAGGTTCACATATACGTAC+AGG - chr4.2:20541775-20541794 None:intergenic 40.0%
CACGTGTTAGGTTTGATGTA+TGG - chr4.2:20540839-20540858 None:intergenic 40.0%
CATATGCCAGATCTACTTAC+TGG + chr4.2:20544431-20544450 MS.gene40157:CDS 40.0%
CCAGATCTACTTACTGGTAA+TGG + chr4.2:20544437-20544456 MS.gene40157:CDS 40.0%
CCATTACCAGTAAGTAGATC+TGG - chr4.2:20544440-20544459 None:intergenic 40.0%
CCCTCTCTTAAAAGCTTTCA+AGG - chr4.2:20542314-20542333 None:intergenic 40.0%
CTACCTTCGTTCCTTACTAT+AGG - chr4.2:20540683-20540702 None:intergenic 40.0%
CTAGTACTGTCACAACTTCA+AGG + chr4.2:20544060-20544079 MS.gene40157:CDS 40.0%
GAAGGTTCTTGCATTGAGAA+GGG + chr4.2:20543329-20543348 MS.gene40157:CDS 40.0%
GAATAGTATGGCTGTTGATG+TGG + chr4.2:20542198-20542217 MS.gene40157:CDS 40.0%
GAGCTATCAGCATTAATACG+CGG + chr4.2:20542808-20542827 MS.gene40157:CDS 40.0%
GATAAGTGATGAGGCTATCA+AGG + chr4.2:20540972-20540991 MS.gene40157:intron 40.0%
GATGCTCATCATGTGCAAAA+TGG - chr4.2:20544313-20544332 None:intergenic 40.0%
GCAAAGACAGGTGAAGAAAA+GGG + chr4.2:20542619-20542638 MS.gene40157:CDS 40.0%
GCATCTTCTTCAAGTACTAC+AGG + chr4.2:20542121-20542140 MS.gene40157:CDS 40.0%
GCTCTCAAAATCTTCCAGTT+GGG - chr4.2:20544204-20544223 None:intergenic 40.0%
GCTCTCTTTGAATCATCAAC+CGG - chr4.2:20543293-20543312 None:intergenic 40.0%
GGAAATTCAGCTAAGGAAAG+CGG + chr4.2:20542363-20542382 MS.gene40157:CDS 40.0%
GGGTCGATATATACTTGAGT+AGG + chr4.2:20541036-20541055 MS.gene40157:intron 40.0%
GTAAGGAGTGAAAGATATGC+TGG - chr4.2:20543546-20543565 None:intergenic 40.0%
GTCACAACTTCAAGGAACAA+AGG + chr4.2:20544068-20544087 MS.gene40157:CDS 40.0%
GTTAACCTTGCGATTGATGA+AGG - chr4.2:20540148-20540167 None:intergenic 40.0%
TAAGCTTGCAGAAGCTAAAG+AGG + chr4.2:20542708-20542727 MS.gene40157:CDS 40.0%
TACTTCAGAACTTCAAGAGC+TGG + chr4.2:20542504-20542523 MS.gene40157:CDS 40.0%
TAGCAACGCTTCTTTGTCAA+AGG - chr4.2:20543034-20543053 None:intergenic 40.0%
TAGGTTTGATGTATGGAGTC+AGG - chr4.2:20540832-20540851 None:intergenic 40.0%
TCAGAGCAGATAAGAGTTGA+GGG - chr4.2:20545132-20545151 None:intergenic 40.0%
TCAGCAAACTTGAGTTTGGA+GGG + chr4.2:20542565-20542584 MS.gene40157:CDS 40.0%
TCCTCAGACTGGTAAAGATA+AGG + chr4.2:20542474-20542493 MS.gene40157:CDS 40.0%
TCCTGAAAGAGTAAGCCATA+AGG + chr4.2:20541367-20541386 MS.gene40157:intron 40.0%
TCCTTATCTTTACCAGTCTG+AGG - chr4.2:20542478-20542497 None:intergenic 40.0%
TCTCCTATAGTAAGGAACGA+AGG + chr4.2:20540677-20540696 MS.gene40157:intron 40.0%
TGATGTGTTGGAAACATTGC+TGG - chr4.2:20544852-20544871 None:intergenic 40.0%
TGCAAAGACAGGTGAAGAAA+AGG + chr4.2:20542618-20542637 MS.gene40157:CDS 40.0%
TGCAGAGGAAGTCCTTAAAA+TGG + chr4.2:20543791-20543810 MS.gene40157:CDS 40.0%
TGCTCATGATCATGCAGATT+TGG - chr4.2:20542859-20542878 None:intergenic 40.0%
TGTTCCAGATTATGCTTCTC+AGG + chr4.2:20544667-20544686 MS.gene40157:CDS 40.0%
TTAGCACAATCGAAGAATGG+GGG - chr4.2:20540171-20540190 None:intergenic 40.0%
TTCACACCTTAATGCATTGC+AGG + chr4.2:20540763-20540782 MS.gene40157:intron 40.0%
TTCAGAGCAGATAAGAGTTG+AGG - chr4.2:20545133-20545152 None:intergenic 40.0%
TTCTTGCATTGAGAAGGGAA+GGG + chr4.2:20543334-20543353 MS.gene40157:CDS 40.0%
TTTCCGTGAGATGATTCAGA+CGG - chr4.2:20544275-20544294 None:intergenic 40.0%
! AATCTAGGATGGAAGGATGA+AGG + chr4.2:20543993-20544012 MS.gene40157:CDS 40.0%
! ACTCTTAGCGTTGAAAAGGA+AGG + chr4.2:20544106-20544125 MS.gene40157:CDS 40.0%
! CTTCAATTCTCCAGTTGATG+TGG + chr4.2:20543896-20543915 MS.gene40157:CDS 40.0%
! GAACTCAAACTTTTCCCAAC+AGG - chr4.2:20543213-20543232 None:intergenic 40.0%
! GAGAACTCTTAGCGTTGAAA+AGG + chr4.2:20544102-20544121 MS.gene40157:CDS 40.0%
! GCAGAAACTTTTGCAAAGAC+AGG + chr4.2:20542607-20542626 MS.gene40157:CDS 40.0%
! GCTTACTAATCTAGGATGGA+AGG + chr4.2:20543986-20544005 MS.gene40157:CDS 40.0%
! GGTTCTTCTTTTATGGTCAC+GGG - chr4.2:20544026-20544045 None:intergenic 40.0%
! TTGGTTAGGGTTCTCGATTT+TGG - chr4.2:20545230-20545249 None:intergenic 40.0%
! TTTAAGACGGGAAGGGAAAA+CGG + chr4.2:20543764-20543783 MS.gene40157:CDS 40.0%
!! AGTTGATGAAGAAAGGGACA+TGG + chr4.2:20543920-20543939 MS.gene40157:CDS 40.0%
!! ATGTGGCAGTTGATGAAGAA+AGG + chr4.2:20543913-20543932 MS.gene40157:CDS 40.0%
!! CAAGCACAGGTTGATTATCA+TGG - chr4.2:20541881-20541900 None:intergenic 40.0%
!! GAAAGCTTTTAAGAGAGGGA+AGG + chr4.2:20542315-20542334 MS.gene40157:CDS 40.0%
!! GCAGTTGCTTTGAAAAGAGA+AGG + chr4.2:20544734-20544753 MS.gene40157:CDS 40.0%
!! GCCTTGAAAGCTTTTAAGAG+AGG + chr4.2:20542310-20542329 MS.gene40157:CDS 40.0%
!! TCAGCAACAAGTCCTTGTTT+TGG - chr4.2:20544226-20544245 None:intergenic 40.0%
!! TGAATCATCAACCGGCTTTT+TGG - chr4.2:20543285-20543304 None:intergenic 40.0%
!! TGTGGCAGTTGATGAAGAAA+GGG + chr4.2:20543914-20543933 MS.gene40157:CDS 40.0%
!! TTATCTGCTCTGAAAGCTAC+TGG + chr4.2:20545139-20545158 MS.gene40157:CDS 40.0%
!! TTTTTCAGCACCACTGTAGA+AGG + chr4.2:20541683-20541702 MS.gene40157:intron 40.0%
!!! ACCCAGCTTTTTTCAAAGCT+TGG + chr4.2:20540902-20540921 MS.gene40157:intron 40.0%
AAAGGCAATTGAAGCGCAGT+TGG + chr4.2:20545036-20545055 MS.gene40157:CDS 45.0%
AACGTCAAGCCCTAAAGCTA+CGG + chr4.2:20544969-20544988 MS.gene40157:CDS 45.0%
ACAAAGTCACTTCCTCAGAC+TGG + chr4.2:20542463-20542482 MS.gene40157:CDS 45.0%
ACCACTCCTCAATGACCTTA+TGG - chr4.2:20541385-20541404 None:intergenic 45.0%
ACCCTGATCTGCTAGGTTAA+AGG - chr4.2:20540363-20540382 None:intergenic 45.0%
ACTCTAACACAAGCAAGCAC+AGG - chr4.2:20541894-20541913 None:intergenic 45.0%
AGAAGTCCTCTAGTTGAGGA+AGG + chr4.2:20543483-20543502 MS.gene40157:CDS 45.0%
AGAGAAGCAACTGGAAGAAC+AGG + chr4.2:20542753-20542772 MS.gene40157:CDS 45.0%
AGATTCACTGAGGAGTCCAA+AGG - chr4.2:20544551-20544570 None:intergenic 45.0%
AGCAGAAGCTGAGTTAGAAC+GGG + chr4.2:20545012-20545031 MS.gene40157:CDS 45.0%
AGGAAAACAAGCTGAAGCCT+TGG + chr4.2:20545315-20545334 MS.gene40157:CDS 45.0%
AGGGTCCATCAAATCCTCAT+CGG - chr4.2:20542944-20542963 None:intergenic 45.0%
AGTGACCGATGAGGATTTGA+TGG + chr4.2:20542936-20542955 MS.gene40157:CDS 45.0%
AGTTCTTGAGCATCAGCTGA+TGG + chr4.2:20543395-20543414 MS.gene40157:CDS 45.0%
ATCAGCATTAATACGCGGCA+TGG + chr4.2:20542813-20542832 MS.gene40157:CDS 45.0%
ATCCACCGCATCTGACTTAT+CGG - chr4.2:20545083-20545102 None:intergenic 45.0%
ATGGATCAAGCTCACGTGTT+AGG - chr4.2:20540851-20540870 None:intergenic 45.0%
ATTGCTCATAGCTCCTCAAC+TGG + chr4.2:20544398-20544417 MS.gene40157:CDS 45.0%
CAACCTCTTCTCTAACAGCT+TGG - chr4.2:20544795-20544814 None:intergenic 45.0%
CAAGCTGTTAGAGAAGAGGT+TGG + chr4.2:20544793-20544812 MS.gene40157:CDS 45.0%
CAATCGTCATCGACGAATAC+TGG + chr4.2:20543438-20543457 MS.gene40157:CDS 45.0%
CACCAAAGCCTTCTTTACGA+GGG + chr4.2:20540070-20540089 MS.gene40157:CDS 45.0%
CACCTTAATGCATTGCAGGT+AGG + chr4.2:20540767-20540786 MS.gene40157:intron 45.0%
CACGCAAAGCCATTCTTTGT+TGG - chr4.2:20541738-20541757 None:intergenic 45.0%
CCAGATTCTTGGTCAAAACG+AGG + chr4.2:20542030-20542049 MS.gene40157:CDS 45.0%
CGGAAAAAGTTGTTGCCTTC+TGG + chr4.2:20542388-20542407 MS.gene40157:CDS 45.0%
CTCCTACCTGCAATGCATTA+AGG - chr4.2:20540772-20540791 None:intergenic 45.0%
CTGATCTGCTAGGTTAAAGG+CGG - chr4.2:20540360-20540379 None:intergenic 45.0%
CTTGTTGCTGAATGCATCCA+AGG + chr4.2:20544235-20544254 MS.gene40157:CDS 45.0%
CTTTCACTCCTTACGGACTT+GGG + chr4.2:20543552-20543571 MS.gene40157:CDS 45.0%
GAAAACGAGATCTTGGGTAC+TGG + chr4.2:20541571-20541590 MS.gene40157:intron 45.0%
GAGGAGTCCAAAGGAATAGT+AGG - chr4.2:20544542-20544561 None:intergenic 45.0%
GAGTAGAAGTCCTCTAGTTG+AGG + chr4.2:20543479-20543498 MS.gene40157:CDS 45.0%
GATCTGGCATATGCATAGAG+TGG - chr4.2:20544424-20544443 None:intergenic 45.0%
GCCTTTAACCTAGCAGATCA+GGG + chr4.2:20540359-20540378 MS.gene40157:intron 45.0%
GCTCTTAATCAAAAGCGAGC+AGG + chr4.2:20543081-20543100 MS.gene40157:CDS 45.0%
GGAAGCCAAGATAGAAGAGA+TGG + chr4.2:20543824-20543843 MS.gene40157:CDS 45.0%
GGCTCTCAAAATCTTCCAGT+TGG - chr4.2:20544205-20544224 None:intergenic 45.0%
GTCAGCAAACTTGAGTTTGG+AGG + chr4.2:20542564-20542583 MS.gene40157:CDS 45.0%
GTTCCGTCTGAATCATCTCA+CGG + chr4.2:20544269-20544288 MS.gene40157:CDS 45.0%
GTTCTTCCAGTTGCTTCTCT+AGG - chr4.2:20542753-20542772 None:intergenic 45.0%
GTTCTTGCATTGAGAAGGGA+AGG + chr4.2:20543333-20543352 MS.gene40157:CDS 45.0%
TAATGCATTGCAGGTAGGAG+TGG + chr4.2:20540772-20540791 MS.gene40157:intron 45.0%
TAGCACAATCGAAGAATGGG+GGG - chr4.2:20540170-20540189 None:intergenic 45.0%
TATATGGACCCTGATCTGCT+AGG - chr4.2:20540370-20540389 None:intergenic 45.0%
TCATCAACTGCCACATCAAC+TGG - chr4.2:20543909-20543928 None:intergenic 45.0%
TCTTTCACTCCTTACGGACT+TGG + chr4.2:20543551-20543570 MS.gene40157:CDS 45.0%
TGAGCATCCATTATCTGCTG+AGG + chr4.2:20544325-20544344 MS.gene40157:CDS 45.0%
TGATCTGCTAGGTTAAAGGC+GGG - chr4.2:20540359-20540378 None:intergenic 45.0%
TGTGCAAAATGGGAGTTTGC+AGG - chr4.2:20544302-20544321 None:intergenic 45.0%
TGTGTTGGAAACATTGCTGG+TGG - chr4.2:20544849-20544868 None:intergenic 45.0%
TTAGAGAAGAGGTTGGAGGA+TGG + chr4.2:20544800-20544819 MS.gene40157:CDS 45.0%
TTCCCTCGTAAAGAAGGCTT+TGG - chr4.2:20540075-20540094 None:intergenic 45.0%
TTCGTCGATGACGATTGACT+CGG - chr4.2:20543435-20543454 None:intergenic 45.0%
TTGCAGGTAGGAGTGGAAAT+AGG + chr4.2:20540779-20540798 MS.gene40157:intron 45.0%
! CAACAAAGAATGGCTTTGCG+TGG + chr4.2:20541736-20541755 MS.gene40157:CDS 45.0%
! CAACCTCAGTCTTGTCAATC+CGG - chr4.2:20542648-20542667 None:intergenic 45.0%
! GGACATGGTTGTTTCAGAAG+AGG + chr4.2:20543935-20543954 MS.gene40157:CDS 45.0%
! GTTACGTCAGCAAGCTTTAG+CGG + chr4.2:20542240-20542259 MS.gene40157:CDS 45.0%
! GTTGCCTTCTGGAAACATGT+CGG + chr4.2:20542399-20542418 MS.gene40157:CDS 45.0%
! TCGTCCTGAGAAGCATAATC+TGG - chr4.2:20544674-20544693 None:intergenic 45.0%
!! AAAGGAAGGCACTTGCTTTG+AGG + chr4.2:20544120-20544139 MS.gene40157:CDS 45.0%
!! AAAGGCCCTTGCTTTAAGAC+GGG + chr4.2:20543752-20543771 MS.gene40157:CDS 45.0%
!! AGGGTTCTCGATTTTGGCAA+CGG - chr4.2:20545224-20545243 None:intergenic 45.0%
!! CCTCGTTTTGACCAAGAATC+TGG - chr4.2:20542033-20542052 None:intergenic 45.0%
!! GAAAGGCCCTTGCTTTAAGA+CGG + chr4.2:20543751-20543770 MS.gene40157:CDS 45.0%
!! GCCTCTTCTAGCTTCTTACA+AGG - chr4.2:20541793-20541812 None:intergenic 45.0%
!! GCGGTTAGTTTGAAACGATC+AGG + chr4.2:20545295-20545314 MS.gene40157:CDS 45.0%
!! TGACCGTTTCAAGTTGCAAC+AGG + chr4.2:20544931-20544950 MS.gene40157:CDS 45.0%
AACGGCTTTGCAGCAGCATT+CGG - chr4.2:20544902-20544921 None:intergenic 50.0%
ACCAGTGACAAAGGTCGTAG+TGG + chr4.2:20542079-20542098 MS.gene40157:CDS 50.0%
ACGTAACTCATCAGGAGTGG+TGG - chr4.2:20542227-20542246 None:intergenic 50.0%
AGAGCTGGGATGGTCTGATT+TGG + chr4.2:20542519-20542538 MS.gene40157:CDS 50.0%
AGCGGGTCCTACTATTCCTT+TGG + chr4.2:20544532-20544551 MS.gene40157:CDS 50.0%
AGCTGAGTTAGAACGGGCAA+AGG + chr4.2:20545018-20545037 MS.gene40157:CDS 50.0%
AGGCCAAGCTGTTAGAGAAG+AGG + chr4.2:20544789-20544808 MS.gene40157:CDS 50.0%
ATGCAGCGCTAACTGATCTC+TGG - chr4.2:20542105-20542124 None:intergenic 50.0%
ATGCATAGAGTGGCCAGTTG+AGG - chr4.2:20544414-20544433 None:intergenic 50.0%
CAAGAATCTGGAAGCCCAAC+TGG + chr4.2:20544187-20544206 MS.gene40157:CDS 50.0%
CAGAACTTCAAGAGCTGGGA+TGG + chr4.2:20542509-20542528 MS.gene40157:CDS 50.0%
CAGAATCTTCAGCACCAGCA+AGG - chr4.2:20542783-20542802 None:intergenic 50.0%
CAGCTTCTGCTTCCTCTGTT+CGG - chr4.2:20545003-20545022 None:intergenic 50.0%
CAGTTGGGCTTCCAGATTCT+TGG - chr4.2:20544189-20544208 None:intergenic 50.0%
CATTCCAACCCAAGTCCGTA+AGG - chr4.2:20543563-20543582 None:intergenic 50.0%
CCACCAAAGCCTTCTTTACG+AGG + chr4.2:20540069-20540088 MS.gene40157:CDS 50.0%
CCACTTCCTTCCTCAACTAG+AGG - chr4.2:20543492-20543511 None:intergenic 50.0%
CCCTTCCCGTCTTAAAGCAA+GGG - chr4.2:20543760-20543779 None:intergenic 50.0%
CCTCTAGTTGAGGAAGGAAG+TGG + chr4.2:20543489-20543508 MS.gene40157:CDS 50.0%
CGCCTTTAACCTAGCAGATC+AGG + chr4.2:20540358-20540377 MS.gene40157:intron 50.0%
CTGACGTAACTCATCAGGAG+TGG - chr4.2:20542230-20542249 None:intergenic 50.0%
GAAAGGAGACGGGAAATCTG+AGG + chr4.2:20542285-20542304 MS.gene40157:CDS 50.0%
GAAGAACAGGAACTCCTTGC+TGG + chr4.2:20542766-20542785 MS.gene40157:CDS 50.0%
GCACTCACCTCAGCAGATAA+TGG - chr4.2:20544335-20544354 None:intergenic 50.0%
GCCATAAGGTCATTGAGGAG+TGG + chr4.2:20541381-20541400 MS.gene40157:intron 50.0%
GCGCAAGGGAGAAATAGAAG+AGG + chr4.2:20544142-20544161 MS.gene40157:CDS 50.0%
GCTCTAACGTTGAAGCGTGA+GGG + chr4.2:20542685-20542704 MS.gene40157:CDS 50.0%
GCTGTTAGAGAAGAGGTTGG+AGG + chr4.2:20544796-20544815 MS.gene40157:CDS 50.0%
GGATGCCGATAAGTCAGATG+CGG + chr4.2:20545075-20545094 MS.gene40157:CDS 50.0%
GGGAGAAATAGAAGAGGCTG+AGG + chr4.2:20544148-20544167 MS.gene40157:CDS 50.0%
GTCTTGGCTGAGATTCACTG+AGG - chr4.2:20544561-20544580 None:intergenic 50.0%
GTTGATGCCTCCAATTGCCA+GGG + chr4.2:20540105-20540124 MS.gene40157:CDS 50.0%
GTTGGAAACATTGCTGGTGG+AGG - chr4.2:20544846-20544865 None:intergenic 50.0%
TAAAGGCGGGTAAGGTTGCA+CGG - chr4.2:20540346-20540365 None:intergenic 50.0%
TCCCTTCCCGTCTTAAAGCA+AGG - chr4.2:20543761-20543780 None:intergenic 50.0%
TGAATTGAGCGGAACAACCC+TGG - chr4.2:20540125-20540144 None:intergenic 50.0%
TGAGAGGCAAGCAGATGCTT+TGG + chr4.2:20542342-20542361 MS.gene40157:CDS 50.0%
TGAGCATCAGCTGATGGAGA+TGG + chr4.2:20543401-20543420 MS.gene40157:CDS 50.0%
TGCTAGGTTAAAGGCGGGTA+AGG - chr4.2:20540354-20540373 None:intergenic 50.0%
TTCAGCTAAGGAAAGCGGCT+CGG + chr4.2:20542368-20542387 MS.gene40157:CDS 50.0%
! AAGAATGGCTTTGCGTGGAC+AGG + chr4.2:20541741-20541760 MS.gene40157:CDS 50.0%
! AGAATGGCTTTGCGTGGACA+GGG + chr4.2:20541742-20541761 MS.gene40157:CDS 50.0%
! AGCACCACTGTAGAAGGTGT+GGG + chr4.2:20541689-20541708 MS.gene40157:intron 50.0%
! AGGTGTGGGGGTCTATTTTG+CGG + chr4.2:20541703-20541722 MS.gene40157:CDS 50.0%
! CCAAAGCACCAGCTAACTCA+GGG - chr4.2:20542963-20542982 None:intergenic 50.0%
! CCTCGTAAAGAAGGCTTTGG+TGG - chr4.2:20540072-20540091 None:intergenic 50.0%
! GCCATCTCTCCGTAGCTTTA+GGG - chr4.2:20544981-20545000 None:intergenic 50.0%
! GCTTGCTGACGTAACTCATC+AGG - chr4.2:20542235-20542254 None:intergenic 50.0%
! GGTGGATGATGTGAGTGTTG+AGG + chr4.2:20545096-20545115 MS.gene40157:CDS 50.0%
! TAATAATGCCGCTTCCACGG+TGG + chr4.2:20543257-20543276 MS.gene40157:CDS 50.0%
! TCCAAAGCACCAGCTAACTC+AGG - chr4.2:20542964-20542983 None:intergenic 50.0%
! TTGATGGACCCTGAGTTAGC+TGG + chr4.2:20542952-20542971 MS.gene40157:CDS 50.0%
!! ACGAGATCTTGGGTACTGGA+AGG + chr4.2:20541575-20541594 MS.gene40157:intron 50.0%
!! AGATCTTGGGTACTGGAAGG+TGG + chr4.2:20541578-20541597 MS.gene40157:intron 50.0%
!! AGCTGGTGCTTTGGAATCAC+TGG + chr4.2:20542969-20542988 MS.gene40157:CDS 50.0%
!! CCCTTGCTTTAAGACGGGAA+GGG + chr4.2:20543757-20543776 MS.gene40157:CDS 50.0%
!! GCTGGTGCTTTGGAATCACT+GGG + chr4.2:20542970-20542989 MS.gene40157:CDS 50.0%
!! GGGTTCTCGATTTTGGCAAC+GGG - chr4.2:20545223-20545242 None:intergenic 50.0%
!! GGTGCTGATTTCAAGCTTCC+TGG - chr4.2:20541986-20542005 None:intergenic 50.0%
!! GTGCTTTGGAATCACTGGGT+TGG + chr4.2:20542974-20542993 MS.gene40157:CDS 50.0%
AAGACGGGAAGGGAAAACGG+AGG + chr4.2:20543767-20543786 MS.gene40157:CDS 55.0%
ACGGCTTTGCAGCAGCATTC+GGG - chr4.2:20544901-20544920 None:intergenic 55.0%
AGAGAGGGAAGGAGCTTGAG+AGG + chr4.2:20542326-20542345 MS.gene40157:CDS 55.0%
AGCGGAACAACCCTGGCAAT+TGG - chr4.2:20540118-20540137 None:intergenic 55.0%
AGGGAAAACGGAGGATGCAG+AGG + chr4.2:20543776-20543795 MS.gene40157:CDS 55.0%
CACTCCTTACGGACTTGGGT+TGG + chr4.2:20543556-20543575 MS.gene40157:CDS 55.0%
CAGCACCACTGTAGAAGGTG+TGG + chr4.2:20541688-20541707 MS.gene40157:intron 55.0%
CAGCCGGATTGACAAGACTG+AGG + chr4.2:20542642-20542661 MS.gene40157:CDS 55.0%
CAGGTGAAGAAAAGGGCAGC+CGG + chr4.2:20542626-20542645 MS.gene40157:CDS 55.0%
CCCTGAGTTAGCTGGTGCTT+TGG + chr4.2:20542960-20542979 MS.gene40157:CDS 55.0%
CGTTGATGCCTCCAATTGCC+AGG + chr4.2:20540104-20540123 MS.gene40157:CDS 55.0%
GACTTCTACTCGCTGCATGC+AGG - chr4.2:20543470-20543489 None:intergenic 55.0%
GCCACTACGACCTTTGTCAC+TGG - chr4.2:20542083-20542102 None:intergenic 55.0%
GCCCTAAAGCTACGGAGAGA+TGG + chr4.2:20544977-20544996 MS.gene40157:CDS 55.0%
GGAACAACCCTGGCAATTGG+AGG - chr4.2:20540115-20540134 None:intergenic 55.0%
GGCAATTGAAGCGCAGTTGG+AGG + chr4.2:20545039-20545058 MS.gene40157:CDS 55.0%
GGCCATCTCTCCGTAGCTTT+AGG - chr4.2:20544982-20545001 None:intergenic 55.0%
TGAAGCGCAGTTGGAGGAGT+TGG + chr4.2:20545045-20545064 MS.gene40157:CDS 55.0%
TGCCGATAAGTCAGATGCGG+TGG + chr4.2:20545078-20545097 MS.gene40157:CDS 55.0%
TTGCTGGTGGAGGCAGTGTT+TGG - chr4.2:20544836-20544855 None:intergenic 55.0%
! CACCACTGTAGAAGGTGTGG+GGG + chr4.2:20541691-20541710 MS.gene40157:CDS 55.0%
! GCACCACTGTAGAAGGTGTG+GGG + chr4.2:20541690-20541709 MS.gene40157:intron 55.0%
! GGCTCTAACGTTGAAGCGTG+AGG + chr4.2:20542684-20542703 MS.gene40157:CDS 55.0%
!! AGCGCGATTTGAGTTGCGAC+AGG + chr4.2:20541813-20541832 MS.gene40157:CDS 55.0%
!! GCACTTGCTTTGAGGCGCAA+GGG + chr4.2:20544128-20544147 MS.gene40157:CDS 55.0%
!! GCCCTTGCTTTAAGACGGGA+AGG + chr4.2:20543756-20543775 MS.gene40157:CDS 55.0%
!! GCGCGATTTGAGTTGCGACA+GGG + chr4.2:20541814-20541833 MS.gene40157:CDS 55.0%
!!! TTTTGGTGCCACCGTGGAAG+CGG - chr4.2:20543268-20543287 None:intergenic 55.0%
ACGGAGAGATGGCCGAACAG+AGG + chr4.2:20544988-20545007 MS.gene40157:CDS 60.0%
AGCCAGGGACGAACTTCGAC+AGG + chr4.2:20544769-20544788 MS.gene40157:CDS 60.0%
CCACGGTGGCACCAAAAAGC+CGG + chr4.2:20543271-20543290 MS.gene40157:CDS 60.0%
CGGCTTTGCAGCAGCATTCG+GGG - chr4.2:20544900-20544919 None:intergenic 60.0%
GACCCCCACACCTTCTACAG+TGG - chr4.2:20541696-20541715 None:intergenic 60.0%
GTTGGAGGAGTTGGCTGCTC+AGG + chr4.2:20545054-20545073 MS.gene40157:CDS 60.0%
TGAGTTGCGACAGGGACGCA+GGG + chr4.2:20541822-20541841 MS.gene40157:CDS 60.0%
! TTGAGTTGCGACAGGGACGC+AGG + chr4.2:20541821-20541840 MS.gene40157:CDS 60.0%
!! GGCACGTCGAAGAGCATCCA+AGG - chr4.2:20545335-20545354 None:intergenic 60.0%
!! GGCACTTGCTTTGAGGCGCA+AGG + chr4.2:20544127-20544146 MS.gene40157:CDS 60.0%
GGCCTGTCGAAGTTCGTCCC+TGG - chr4.2:20544774-20544793 None:intergenic 65.0%
!!! CCGGCTTTTTGGTGCCACCG+TGG - chr4.2:20543274-20543293 None:intergenic 65.0%
TGCGACAGGGACGCAGGGCT+GGG + chr4.2:20541827-20541846 MS.gene40157:CDS 70.0%
TTGCGACAGGGACGCAGGGC+TGG + chr4.2:20541826-20541845 MS.gene40157:CDS 70.0%
CAGGGACGCAGGGCTGGGAG+AGG + chr4.2:20541832-20541851 MS.gene40157:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 20540048 20545399 20540048 ID=MS.gene40157
chr4.2 mRNA 20540048 20545399 20540048 ID=MS.gene40157.t1;Parent=MS.gene40157
chr4.2 exon 20540048 20540161 20540048 ID=MS.gene40157.t1.exon1;Parent=MS.gene40157.t1
chr4.2 CDS 20540048 20540161 20540048 ID=cds.MS.gene40157.t1;Parent=MS.gene40157.t1
chr4.2 exon 20541691 20541853 20541691 ID=MS.gene40157.t1.exon2;Parent=MS.gene40157.t1
chr4.2 CDS 20541691 20541853 20541691 ID=cds.MS.gene40157.t1;Parent=MS.gene40157.t1
chr4.2 exon 20541987 20545399 20541987 ID=MS.gene40157.t1.exon3;Parent=MS.gene40157.t1
chr4.2 CDS 20541987 20545399 20541987 ID=cds.MS.gene40157.t1;Parent=MS.gene40157.t1
Gene Sequence

>MS.gene40157

ATGTTAGCGAAGATCGGGTTGCCACCAAAGCCTTCTTTACGAGGGAATAATTGGGTCGTTGATGCCTCCAATTGCCAGGGTTGTTCCGCTCAATTCACCTTCATCAATCGCAAGGTTAACCCCCCATTCTTCGATTGTGCTAATCTTATTATTATGACTTAATTTTACCAATCATCAAAGACTCTGTTACTCTATAACCATGATTTGAATTTCAGTGACTTTCACTTTACTGTAATTTGATTTGTTGTATTTTATGAACGATAAATGATAGAGAACAATATAGGAGTGATATGCACCGTGCAACCTTACCCGCCTTTAACCTAGCAGATCAGGGTCCATATACCGATGAATTACATTGCTGCTAATTTATTGCCGTTGCATTAACAAGAGTAATTTTATGCTGTCAAGATTAGAGTCAAATAGTTCACAAGATTTCTTTGCTCTATCAAGCCATGTTTAACAACTTGTCATTTGCTTGCTTAGTTCTTTTTCGAAACAAATTTTGCTTGTTTCATCGTAGGAGCATGTTTACGAGATTTTGAATCTTACTACCCTAGAGACAATTCACTTTTTATGTTCATTGAATAACTAATGTATCTGGTATTCAATGAACATAAAAAAGTGAATTGTCTCCTATAGTAAGGAACGAAGGTAGTATTAATTAATGTGAAGCAAGTGAGAATCAATTTTTCATGCCCGGCTATATTGTTTGTGTTTCACACCTTAATGCATTGCAGGTAGGAGTGGAAATAGGTTATTAGACTAAAATAAATTTTTAAAACCTGACTCCATACATCAAACCTAACACGTGAGCTTGATCCATTTTCTCTTTATAGTTCTTTTTTTAATACTTGACCCAGCTTTTTTCAAAGCTTGGTTTGGCCTTAACCTTATTTAAAAGCCTATTTTGTGATAAGGTCTTTAGATAAGTGATGAGGCTATCAAGGTCTATAGATAAGTGATGAGGCTATCAACAAGTAAGCTCATAGGGTCGATATATACTTGAGTAGGTTAGTTTTGTTAGGTTTAAAGGTTTTTGGATGGTCTAAAGGTTTGTCCTTTTAGCTAAACAGACTTTTGAAAAAGCCTTGACCTATTTTCTTCATTACATAAGTTTTGTTTGGTCTAGGTTTGAGTACATTAGTTTGGTTTGGTCTAGTTTGATGCAGAATAATCATCTACCTCTTCTCTTCATTTAATTTTCTATGGAAATTTGCTTCATGAGAATAGTAATTAAATAACCTTAAATATTGTAAAATTAGTTAGGATTTGCTTATATTTAGACCACCAAATCTATACAATTTTCACTTATCTTTTAGTCCTGAAAGAGTAAGCCATAAGGTCATTGAGGAGTGGTTGACATATTTCCGTCCCTAATTGTATGTTTAGCTTCCCTTAGATTGGATAACTCACTATGTTTTCTATGTTACCCAGTTTTAATTGTTTTATCAAACTTTAAGCTTCATAGAACCTTTGGTTAAACTAAAGGTATAATGTGTTTGTTAATTTCATGATTCTATATGGAAAACGAGATCTTGGGTACTGGAAGGTGGTCTTCCATGTTTGCTTATTATAATCTGATTGAACTCAAACCAAATCCTTTGTCCCAATTAAATTTTGGAATTTTGATTATTATTTTTCAGCACCACTGTAGAAGGTGTGGGGGTCTATTTTGCGGAAGTTGTACCCAACAAAGAATGGCTTTGCGTGGACAGGGTGATTCGCCTGTACGTATATGTGAACCTTGTAAGAAGCTAGAAGAGGCAGCGCGATTTGAGTTGCGACAGGGACGCAGGGCTGGGAGAGGTATCCCCCTCATTTTTGTTAAAACCATGATAATCAACCTGTGCTTGCTTGTGTTAGAGTTTCACTTCATCATTTTGCTTTCTTTATTACTACACAGTTCCATGCTTTTGAATATTCACTCTAATTTCTCCAGGAAGCTTGAAATCAGCACCTAGAGATGAAGATGAAATTCTCAACCAGATTCTTGGTCAAAACGAGGAACTTCTTTTATCAGGAAAGCAATCAACCAGTGACAAAGGTCGTAGTGGCCAGAGATCAGTTAGCGCTGCATCTTCTTCAAGTACTACAGGATTTTCTATTCAGGACGAAGAAGATATACAAAAGATTATATCAACTGAAACAACGAATAGTATGGCTGTTGATGTGGGATCCACCACTCCTGATGAGTTACGTCAGCAAGCTTTAGCGGAAAAAAGTAAGTATAAAATTCTGAAAGGAGACGGGAAATCTGAGGAAGCCTTGAAAGCTTTTAAGAGAGGGAAGGAGCTTGAGAGGCAAGCAGATGCTTTGGAAATTCAGCTAAGGAAAGCGGCTCGGAAAAAGTTGTTGCCTTCTGGAAACATGTCGGATATGCACAACAAAGACATTTCTGTTGAGTCTGGCAGAAAAACAAAGTCACTTCCTCAGACTGGTAAAGATAAGGATGATCTTACTTCAGAACTTCAAGAGCTGGGATGGTCTGATTTGGAACTACACAAAGAAGATAGAAAGTCAGCAAACTTGAGTTTGGAGGGTGAACTCTCCTCCTTGATTGCAGAAACTTTTGCAAAGACAGGTGAAGAAAAGGGCAGCCGGATTGACAAGACTGAGGTTGTTGCTATGAAAAAGAAGGCTCTAACGTTGAAGCGTGAGGGTAAGCTTGCAGAAGCTAAAGAGGAATTGAAAAAAGCAAAAATCCTAGAGAAGCAACTGGAAGAACAGGAACTCCTTGCTGGTGCTGAAGATTCTGATGATGAGCTATCAGCATTAATACGCGGCATGGATGATGATGATAAAGAATTTCCAAATCTGCATGATCATGAGCATGGTTTTGATTTTGATAATCTCTTGGCCATTTCTGATAATCTTGATGGTAATTTAGAAGTGACCGATGAGGATTTGATGGACCCTGAGTTAGCTGGTGCTTTGGAATCACTGGGTTGGACTGAACCTGAAAATACATTTTCCAAATCTCAAACCTTTGACAAAGAAGCGTTGCTAAGTGAAATTCAGTCATTGAAAAGAGAGGCTCTTAATCAAAAGCGAGCAGGTAATACTGAAGAAGCAATGGCGATATTGAAAAAGGCGAAGTTATTAGAAAGTGACTTAAACAACACTGGGTCTGATGACGATAATACTATGTTGCAAAAAGTTACTCCTGTTGGGAAAAGTTTGAGTTCTGAAATTACTGGTAATGATAGAAATAATAATGCCGCTTCCACGGTGGCACCAAAAAGCCGGTTGATGATTCAAAGAGAGCTTTTGAATTCGAAAAAGAAGGTTCTTGCATTGAGAAGGGAAGGGAAAATGGATGAAGCAGAAGAAGAAATGCGGAAGAGTGCAGTTCTTGAGCATCAGCTGATGGAGATGGACAATGCTCCGAGTCAATCGTCATCGACGAATACTGGTAATGTCCTGCATGCAGCGAGTAGAAGTCCTCTAGTTGAGGAAGGAAGTGGCGATGATGTAACAGATAAAGATATGTCTGATCCAGCATATCTTTCACTCCTTACGGACTTGGGTTGGAATGATGACAATGATAAACCTTCTAAATCTTCGAACAATCCTTCGAAGACATATGATGATAACTTTGTTCCAGTTGATGATACTTTTCTGAGTAAGCATTCTACAACTATGTTTGTTGAAGCACCAAGAAAAAGTAAAGCAGAAATTCAGAGGGAACTCTTGAGCTTAAAAAGAAAGGCCCTTGCTTTAAGACGGGAAGGGAAAACGGAGGATGCAGAGGAAGTCCTTAAAATGGCCAAAACATTGGAAGCCAAGATAGAAGAGATGGATGCTCTGAAGAATAATGTACAGGTTGAGGCTCTTAAGAAGAAAGAACTCTTCAATTCTCCAGTTGATGTGGCAGTTGATGAAGAAAGGGACATGGTTGTTTCAGAAGAGGATATGCATGACCCAGCATTGAATTCAATGCTTACTAATCTAGGATGGAAGGATGAAGGATTTGAGCCCGTGACCATAAAAGAAGAACCTGTTAAGGAAGCTACTAGTACTGTCACAACTTCAAGGAACAAAGGAGAGATTCAAAGAGAACTCTTAGCGTTGAAAAGGAAGGCACTTGCTTTGAGGCGCAAGGGAGAAATAGAAGAGGCTGAGGAAATTTTAAAAAAGGCCAAGAATCTGGAAGCCCAACTGGAAGATTTTGAGAGCCAAAACAAGGACTTGTTGCTGAATGCATCCAAGGATAAAAAATCTGTTCCGTCTGAATCATCTCACGGAAAAAGTCCTGCAAACTCCCATTTTGCACATGATGAGCATCCATTATCTGCTGAGGTGAGTGCTTCAAGTGAAAATCTCACAAAAAGGATGAAAGTCGAAAATATCATTGCTCATAGCTCCTCAACTGGCCACTCTATGCATATGCCAGATCTACTTACTGGTAATGGCTGTAGCAGTTCTGAAATTTTGTCTCAAAAACAAAAAGAAGAATATAAAGCAGGTTCAGTTAATTCATCTCAAGCGGGTCCTACTATTCCTTTGGACTCCTCAGTGAATCTCAGCCAAGACCAGATATACAAAAACAACATTCAAACCCAAAGAAGAAAAGAAGTGACTGATGTTGATGAAAAGCCAAACACAAACCAGTCAAATGTTGTTCCAGATTATGCTTCTCAGGACGACCTTTCTTTGCGTCAAGAAATTTTGGCTCATAAAAGGAAGGCAGTTGCTTTGAAAAGAGAAGGAAAACTAACAGAAGCCAGGGACGAACTTCGACAGGCCAAGCTGTTAGAGAAGAGGTTGGAGGATGGAAGTATGCAACCAAACACTGCCTCCACCAGCAATGTTTCCAACACATCAAATGTTGTTCAAAAGAAACAAGATTCCCCGAATGCTGCTGCAAAGCCGTTTACTAGTCGTGACCGTTTCAAGTTGCAACAGGAGTCTCTAGCACACAAACGTCAAGCCCTAAAGCTACGGAGAGATGGCCGAACAGAGGAAGCAGAAGCTGAGTTAGAACGGGCAAAGGCAATTGAAGCGCAGTTGGAGGAGTTGGCTGCTCAGGATGCCGATAAGTCAGATGCGGTGGATGATGTGAGTGTTGAGGATTTTCTTGACCCTCAACTCTTATCTGCTCTGAAAGCTACTGGACTTGCAGATTTAACTGTTGTATCTAATAAAAGCCCAGAAAAACAAGAGACTGTGAAACCCGTTGCCAAAATCGAGAACCCTAACCAAGAGAAAATTCAGCTAGAAGAAAGAATTAAAGAAGAAAAACTGAAGGCGGTTAGTTTGAAACGATCAGGAAAACAAGCTGAAGCCTTGGATGCTCTTCGACGTGCCAAAATGTATGAAAAGAAGCTGAATTCATTGTTAACATCTGGGTGA
Protein sequence

>MS.gene40157.t1

MLAKIGLPPKPSLRGNNWVVDASNCQGCSAQFTFINRKHHCRRCGGLFCGSCTQQRMALRGQGDSPVRICEPCKKLEEAARFELRQGRRAGRGSLKSAPRDEDEILNQILGQNEELLLSGKQSTSDKGRSGQRSVSAASSSSTTGFSIQDEEDIQKIISTETTNSMAVDVGSTTPDELRQQALAEKSKYKILKGDGKSEEALKAFKRGKELERQADALEIQLRKAARKKLLPSGNMSDMHNKDISVESGRKTKSLPQTGKDKDDLTSELQELGWSDLELHKEDRKSANLSLEGELSSLIAETFAKTGEEKGSRIDKTEVVAMKKKALTLKREGKLAEAKEELKKAKILEKQLEEQELLAGAEDSDDELSALIRGMDDDDKEFPNLHDHEHGFDFDNLLAISDNLDGNLEVTDEDLMDPELAGALESLGWTEPENTFSKSQTFDKEALLSEIQSLKREALNQKRAGNTEEAMAILKKAKLLESDLNNTGSDDDNTMLQKVTPVGKSLSSEITGNDRNNNAASTVAPKSRLMIQRELLNSKKKVLALRREGKMDEAEEEMRKSAVLEHQLMEMDNAPSQSSSTNTGNVLHAASRSPLVEEGSGDDVTDKDMSDPAYLSLLTDLGWNDDNDKPSKSSNNPSKTYDDNFVPVDDTFLSKHSTTMFVEAPRKSKAEIQRELLSLKRKALALRREGKTEDAEEVLKMAKTLEAKIEEMDALKNNVQVEALKKKELFNSPVDVAVDEERDMVVSEEDMHDPALNSMLTNLGWKDEGFEPVTIKEEPVKEATSTVTTSRNKGEIQRELLALKRKALALRRKGEIEEAEEILKKAKNLEAQLEDFESQNKDLLLNASKDKKSVPSESSHGKSPANSHFAHDEHPLSAEVSASSENLTKRMKVENIIAHSSSTGHSMHMPDLLTGNGCSSSEILSQKQKEEYKAGSVNSSQAGPTIPLDSSVNLSQDQIYKNNIQTQRRKEVTDVDEKPNTNQSNVVPDYASQDDLSLRQEILAHKRKAVALKREGKLTEARDELRQAKLLEKRLEDGSMQPNTASTSNVSNTSNVVQKKQDSPNAAAKPFTSRDRFKLQQESLAHKRQALKLRRDGRTEEAEAELERAKAIEAQLEELAAQDADKSDAVDDVSVEDFLDPQLLSALKATGLADLTVVSNKSPEKQETVKPVAKIENPNQEKIQLEERIKEEKLKAVSLKRSGKQAEALDALRRAKMYEKKLNSLLTSG