Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36854.t1 | XP_024629011.1 | 100 | 384 | 0 | 0 | 45 | 428 | 1 | 384 | 4.10E-217 | 763.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36854.t1 | Q949Y0 | 77.3 | 384 | 86 | 1 | 45 | 428 | 1 | 383 | 1.2e-169 | 597.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36854.t1 | A0A396GGA4 | 100.0 | 384 | 0 | 0 | 45 | 428 | 1 | 384 | 3.0e-217 | 763.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050786 | MS.gene36854 | 0.833793 | 4.35E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene27247 | MS.gene36854 | PPI |
| MS.gene24253 | MS.gene36854 | PPI |
| MS.gene90698 | MS.gene36854 | PPI |
| MS.gene06645 | MS.gene36854 | PPI |
| MS.gene36854 | MS.gene050252 | PPI |
| MS.gene044013 | MS.gene36854 | PPI |
| MS.gene062770 | MS.gene36854 | PPI |
| MS.gene007189 | MS.gene36854 | PPI |
| MS.gene36854 | MS.gene81765 | PPI |
| MS.gene057206 | MS.gene36854 | PPI |
| MS.gene29256 | MS.gene36854 | PPI |
| MS.gene36854 | MS.gene26985 | PPI |
| MS.gene36854 | MS.gene24252 | PPI |
| MS.gene36854 | MS.gene24375 | PPI |
| MS.gene36854 | MS.gene27289 | PPI |
| MS.gene06859 | MS.gene36854 | PPI |
| MS.gene67099 | MS.gene36854 | PPI |
| MS.gene05119 | MS.gene36854 | PPI |
| MS.gene050290 | MS.gene36854 | PPI |
| MS.gene53875 | MS.gene36854 | PPI |
| MS.gene24255 | MS.gene36854 | PPI |
| MS.gene013534 | MS.gene36854 | PPI |
| MS.gene004328 | MS.gene36854 | PPI |
| MS.gene36854 | MS.gene034846 | PPI |
| MS.gene008431 | MS.gene36854 | PPI |
| MS.gene24706 | MS.gene36854 | PPI |
| MS.gene002648 | MS.gene36854 | PPI |
| MS.gene36854 | MS.gene049599 | PPI |
| MS.gene36854 | MS.gene056896 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36854.t1 | MTR_8g015660 | 100.000 | 381 | 0 | 0 | 48 | 428 | 78 | 458 | 0.0 | 795 |
| MS.gene36854.t1 | MTR_3g100480 | 27.572 | 243 | 152 | 8 | 149 | 379 | 585 | 815 | 9.17e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36854.t1 | AT3G21280 | 78.182 | 385 | 82 | 2 | 44 | 428 | 55 | 437 | 0.0 | 611 |
| MS.gene36854.t1 | AT3G21280 | 77.865 | 384 | 83 | 2 | 45 | 428 | 1 | 382 | 0.0 | 607 |
| MS.gene36854.t1 | AT1G51710 | 77.344 | 384 | 86 | 1 | 45 | 428 | 1 | 383 | 0.0 | 600 |
| MS.gene36854.t1 | AT1G51710 | 77.015 | 335 | 76 | 1 | 94 | 428 | 11 | 344 | 0.0 | 519 |
Find 79 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTCTTCCACCATGATTAA+TGG | 0.190178 | 8.1:+81885799 | MS.gene36854:CDS |
| ATCAAAGCCGACTTCAATTC+TGG | 0.197702 | 8.1:-81888126 | None:intergenic |
| CTGTGGCACCTATGCAATTT+TGG | 0.236161 | 8.1:+81888346 | MS.gene36854:CDS |
| GGAAAAGGCTTCTGCTGCTT+TGG | 0.253240 | 8.1:+81892373 | MS.gene36854:CDS |
| CTCGCAAAATCTTAGCCTTT+TGG | 0.261860 | 8.1:-81893665 | None:intergenic |
| TTCAGTTTGTCCGTTTCTTT+TGG | 0.281449 | 8.1:+81893628 | MS.gene36854:CDS |
| GTTATCAGTTACTCACATTC+AGG | 0.296836 | 8.1:+81888237 | MS.gene36854:intron |
| AGCTTTCTTCATTGCTCTCT+TGG | 0.309856 | 8.1:-81891016 | None:intergenic |
| AGTTGAGAAGAGGTTGTTCT+TGG | 0.329025 | 8.1:-81885740 | None:intergenic |
| GAAAAGGCTTCTGCTGCTTT+GGG | 0.331458 | 8.1:+81892374 | MS.gene36854:CDS |
| TCCTGAAAGACAAAAGATTA+TGG | 0.342483 | 8.1:+81886807 | MS.gene36854:CDS |
| GCAGCTACTTGTTCTTCTTC+AGG | 0.354152 | 8.1:-81887211 | None:intergenic |
| AAACCACACACACTTATAGA+TGG | 0.371091 | 8.1:-81885685 | None:intergenic |
| AACAAAGTGGATACCTTTCT+AGG | 0.395937 | 8.1:-81893871 | None:intergenic |
| ATCTATAAGTGTGTGTGGTT+TGG | 0.396907 | 8.1:+81885687 | MS.gene36854:CDS |
| CAATTTGGCCAGCTGCATAA+TGG | 0.409917 | 8.1:+81889493 | MS.gene36854:CDS |
| GATGCTGATTGGGCAAAGTT+AGG | 0.423425 | 8.1:+81887005 | MS.gene36854:CDS |
| AAGAGAATAATAACCTTGGC+AGG | 0.430651 | 8.1:-81892436 | None:intergenic |
| GGGTCAAAAGCTAATGATGA+TGG | 0.432476 | 8.1:+81887129 | MS.gene36854:intron |
| TAATGGAGTGTTCATGCAAC+AGG | 0.437555 | 8.1:+81889510 | MS.gene36854:CDS |
| TTTAAAATCAGAGTTGGAAA+AGG | 0.450330 | 8.1:+81892358 | MS.gene36854:CDS |
| CTCTAAGATCCCCTGGATCT+AGG | 0.452923 | 8.1:+81889667 | MS.gene36854:CDS |
| CAATGCAGGATGATGCTGAT+TGG | 0.460308 | 8.1:+81886994 | MS.gene36854:intron |
| TCTGCATTTGCCAGGTATTG+AGG | 0.461481 | 8.1:+81889458 | MS.gene36854:intron |
| CTCCAGTCAGATCAAACAAT+TGG | 0.463837 | 8.1:-81886780 | None:intergenic |
| GGTTGTTCTTGGCTTGAAGA+AGG | 0.464254 | 8.1:-81885729 | None:intergenic |
| GAAGAAAGTTACCTAGATCC+AGG | 0.470982 | 8.1:-81889678 | None:intergenic |
| AACAGGATGCTGAAGAATGC+TGG | 0.479110 | 8.1:+81889616 | MS.gene36854:intron |
| GCAGGGTTTAAAATCAGAGT+TGG | 0.484857 | 8.1:+81892352 | MS.gene36854:intron |
| ATTCCCTCTTCCAAAAGAAA+CGG | 0.501115 | 8.1:-81893638 | None:intergenic |
| TCAAAGCCGACTTCAATTCT+GGG | 0.506445 | 8.1:-81888125 | None:intergenic |
| TCACAATCTCTAAGATCCCC+TGG | 0.506632 | 8.1:+81889660 | MS.gene36854:CDS |
| TTTCTGCAGTGAGTGTTAAG+TGG | 0.507754 | 8.1:+81886695 | MS.gene36854:intron |
| AGAAATCGAACCATTAATCA+TGG | 0.509118 | 8.1:-81885809 | None:intergenic |
| GTTGTTCTTGGCTTGAAGAA+GGG | 0.509936 | 8.1:-81885728 | None:intergenic |
| GAAATTAGAAGCTCCTAGAA+AGG | 0.510977 | 8.1:+81893858 | MS.gene36854:CDS |
| AATGCAGGATGATGCTGATT+GGG | 0.515628 | 8.1:+81886995 | MS.gene36854:intron |
| TTGTTCTTGGCTTGAAGAAG+GGG | 0.518818 | 8.1:-81885727 | None:intergenic |
| GAGTGAGAAAAGTTGAGAAG+AGG | 0.520392 | 8.1:-81885750 | None:intergenic |
| TGCCAATTGTTTGATCTGAC+TGG | 0.522079 | 8.1:+81886778 | MS.gene36854:CDS |
| GGTTTACCTCCTGCGATATG+TGG | 0.524877 | 8.1:-81891070 | None:intergenic |
| TTGGCATAGAACTTAATAGC+AGG | 0.531618 | 8.1:+81890012 | MS.gene36854:CDS |
| GTGGGACAGGGACATACTGC+TGG | 0.532272 | 8.1:+81888042 | MS.gene36854:intron |
| AAGACAAAAGATTATGGTCA+AGG | 0.535017 | 8.1:+81886813 | MS.gene36854:CDS |
| AGTAGATTATCCTCTGGAGT+TGG | 0.535111 | 8.1:+81893801 | MS.gene36854:CDS |
| TGATAAAAGTGTCAAGGCTG+TGG | 0.537101 | 8.1:+81888329 | MS.gene36854:CDS |
| ACTTCAATTCTGGGACAGAA+TGG | 0.537272 | 8.1:-81888116 | None:intergenic |
| GAAGAGGGAATCAAACCAAA+AGG | 0.537832 | 8.1:+81893650 | MS.gene36854:CDS |
| TGAAAATACTGATGCTGTGA+AGG | 0.539297 | 8.1:+81889983 | MS.gene36854:CDS |
| TGAACACTCCATTATGCAGC+TGG | 0.540413 | 8.1:-81889501 | None:intergenic |
| AGTGAGTGTTAAGTGGCAAA+AGG | 0.543381 | 8.1:+81886702 | MS.gene36854:intron |
| ATTATGAAATCTCCAGAGAA+GGG | 0.549279 | 8.1:+81887166 | MS.gene36854:CDS |
| TTATGCAGCTGGCCAAATTG+AGG | 0.549295 | 8.1:-81889490 | None:intergenic |
| ACTTAAATGCCACATATCGC+AGG | 0.558324 | 8.1:+81891061 | MS.gene36854:CDS |
| AAGAAAGTTACCTAGATCCA+GGG | 0.559913 | 8.1:-81889677 | None:intergenic |
| CGCGCATCAATGCCCTGCCA+AGG | 0.566093 | 8.1:+81892423 | MS.gene36854:CDS |
| AATTATGAAATCTCCAGAGA+AGG | 0.574663 | 8.1:+81887165 | MS.gene36854:CDS |
| AACCACACACACTTATAGAT+GGG | 0.582296 | 8.1:-81885684 | None:intergenic |
| TCAGAAAGTAGATTATCCTC+TGG | 0.583259 | 8.1:+81893795 | MS.gene36854:intron |
| GAATAAGTCACGGGTTGCAA+CGG | 0.585557 | 8.1:-81888298 | None:intergenic |
| TGAGAAAAGCTCTGTTCCCA+AGG | 0.585888 | 8.1:+81894035 | MS.gene36854:CDS |
| TATACCATCCAAAATTGCAT+AGG | 0.592018 | 8.1:-81888354 | None:intergenic |
| AGACAAAAGATTATGGTCAA+GGG | 0.594358 | 8.1:+81886814 | MS.gene36854:CDS |
| CAAGCTCGCTGAATAAGTCA+CGG | 0.595928 | 8.1:-81888308 | None:intergenic |
| GGTCAAAAGCTAATGATGAT+GGG | 0.606310 | 8.1:+81887130 | MS.gene36854:CDS |
| TTCTGTCCCAGAATTGAAGT+CGG | 0.608208 | 8.1:+81888119 | MS.gene36854:CDS |
| AGAGAATAATAACCTTGGCA+GGG | 0.620428 | 8.1:-81892435 | None:intergenic |
| CGAGCTTGATAAAAGTGTCA+AGG | 0.622464 | 8.1:+81888323 | MS.gene36854:CDS |
| AAGCTCGCTGAATAAGTCAC+GGG | 0.622507 | 8.1:-81888307 | None:intergenic |
| GGCAAAGTTAGGAGTGAAAG+CGG | 0.624183 | 8.1:+81887016 | MS.gene36854:CDS |
| AATCGAACCATTAATCATGG+TGG | 0.640991 | 8.1:-81885806 | None:intergenic |
| CAAAAGATTATGGTCAAGGG+TGG | 0.645610 | 8.1:+81886817 | MS.gene36854:CDS |
| CAGTTCCTAAGAGATGAAGA+AGG | 0.648671 | 8.1:+81893988 | MS.gene36854:intron |
| GACCCATCTATAAGTGTGTG+TGG | 0.650153 | 8.1:+81885682 | MS.gene36854:CDS |
| TCAATTCTGGGACAGAATGG+AGG | 0.654979 | 8.1:-81888113 | None:intergenic |
| TCGTAAACATCCAACTCCAG+AGG | 0.671783 | 8.1:-81893811 | None:intergenic |
| GAGGTAAACCATTTGCACGA+AGG | 0.687364 | 8.1:+81891083 | MS.gene36854:CDS |
| AGAAAGTTACCTAGATCCAG+GGG | 0.703050 | 8.1:-81889676 | None:intergenic |
| TAAATGCCACATATCGCAGG+AGG | 0.705771 | 8.1:+81891064 | MS.gene36854:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 81885679 | 81894086 | 81885679 | ID=MS.gene36854 |
| chr8.1 | mRNA | 81885679 | 81894086 | 81885679 | ID=MS.gene36854.t1;Parent=MS.gene36854 |
| chr8.1 | exon | 81885679 | 81885820 | 81885679 | ID=MS.gene36854.t1.exon1;Parent=MS.gene36854.t1 |
| chr8.1 | CDS | 81885679 | 81885820 | 81885679 | ID=cds.MS.gene36854.t1;Parent=MS.gene36854.t1 |
| chr8.1 | exon | 81886704 | 81886849 | 81886704 | ID=MS.gene36854.t1.exon2;Parent=MS.gene36854.t1 |
| chr8.1 | CDS | 81886704 | 81886849 | 81886704 | ID=cds.MS.gene36854.t1;Parent=MS.gene36854.t1 |
| chr8.1 | exon | 81887002 | 81887037 | 81887002 | ID=MS.gene36854.t1.exon3;Parent=MS.gene36854.t1 |
| chr8.1 | CDS | 81887002 | 81887037 | 81887002 | ID=cds.MS.gene36854.t1;Parent=MS.gene36854.t1 |
| chr8.1 | exon | 81887130 | 81887237 | 81887130 | ID=MS.gene36854.t1.exon4;Parent=MS.gene36854.t1 |
| chr8.1 | CDS | 81887130 | 81887237 | 81887130 | ID=cds.MS.gene36854.t1;Parent=MS.gene36854.t1 |
| chr8.1 | exon | 81888051 | 81888151 | 81888051 | ID=MS.gene36854.t1.exon5;Parent=MS.gene36854.t1 |
| chr8.1 | CDS | 81888051 | 81888151 | 81888051 | ID=cds.MS.gene36854.t1;Parent=MS.gene36854.t1 |
| chr8.1 | exon | 81888245 | 81888371 | 81888245 | ID=MS.gene36854.t1.exon6;Parent=MS.gene36854.t1 |
| chr8.1 | CDS | 81888245 | 81888371 | 81888245 | ID=cds.MS.gene36854.t1;Parent=MS.gene36854.t1 |
| chr8.1 | exon | 81889472 | 81889531 | 81889472 | ID=MS.gene36854.t1.exon7;Parent=MS.gene36854.t1 |
| chr8.1 | CDS | 81889472 | 81889531 | 81889472 | ID=cds.MS.gene36854.t1;Parent=MS.gene36854.t1 |
| chr8.1 | exon | 81889621 | 81889688 | 81889621 | ID=MS.gene36854.t1.exon8;Parent=MS.gene36854.t1 |
| chr8.1 | CDS | 81889621 | 81889688 | 81889621 | ID=cds.MS.gene36854.t1;Parent=MS.gene36854.t1 |
| chr8.1 | exon | 81889983 | 81890033 | 81889983 | ID=MS.gene36854.t1.exon9;Parent=MS.gene36854.t1 |
| chr8.1 | CDS | 81889983 | 81890033 | 81889983 | ID=cds.MS.gene36854.t1;Parent=MS.gene36854.t1 |
| chr8.1 | exon | 81891007 | 81891115 | 81891007 | ID=MS.gene36854.t1.exon10;Parent=MS.gene36854.t1 |
| chr8.1 | CDS | 81891007 | 81891115 | 81891007 | ID=cds.MS.gene36854.t1;Parent=MS.gene36854.t1 |
| chr8.1 | exon | 81892356 | 81892444 | 81892356 | ID=MS.gene36854.t1.exon11;Parent=MS.gene36854.t1 |
| chr8.1 | CDS | 81892356 | 81892444 | 81892356 | ID=cds.MS.gene36854.t1;Parent=MS.gene36854.t1 |
| chr8.1 | exon | 81893617 | 81893686 | 81893617 | ID=MS.gene36854.t1.exon12;Parent=MS.gene36854.t1 |
| chr8.1 | CDS | 81893617 | 81893686 | 81893617 | ID=cds.MS.gene36854.t1;Parent=MS.gene36854.t1 |
| chr8.1 | exon | 81893799 | 81893879 | 81893799 | ID=MS.gene36854.t1.exon13;Parent=MS.gene36854.t1 |
| chr8.1 | CDS | 81893799 | 81893879 | 81893799 | ID=cds.MS.gene36854.t1;Parent=MS.gene36854.t1 |
| chr8.1 | exon | 81893991 | 81894086 | 81893991 | ID=MS.gene36854.t1.exon14;Parent=MS.gene36854.t1 |
| chr8.1 | CDS | 81893991 | 81894086 | 81893991 | ID=cds.MS.gene36854.t1;Parent=MS.gene36854.t1 |
| Gene Sequence |
| Protein sequence |
