Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60144.t1 | XP_003609377.2 | 99 | 97 | 1 | 0 | 1 | 97 | 28 | 124 | 3.00E-46 | 194.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60144.t1 | Q94AI7 | 88.7 | 97 | 11 | 0 | 1 | 97 | 28 | 124 | 3.4e-43 | 175.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60144.t1 | A0A072USP8 | 99.0 | 97 | 1 | 0 | 1 | 97 | 28 | 124 | 2.1e-46 | 194.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene60144 | 0.81982 | 9.50E-53 | -1.69E-46 |
| MS.gene04915 | MS.gene60144 | 0.804727 | 1.88E-49 | -1.69E-46 |
| MS.gene04917 | MS.gene60144 | 0.810868 | 9.30E-51 | -1.69E-46 |
| MS.gene049208 | MS.gene60144 | 0.803456 | 3.46E-49 | -1.69E-46 |
| MS.gene049887 | MS.gene60144 | 0.808969 | 2.38E-50 | -1.69E-46 |
| MS.gene050084 | MS.gene60144 | 0.822299 | 2.55E-53 | -1.69E-46 |
| MS.gene050085 | MS.gene60144 | 0.83548 | 1.64E-56 | -1.69E-46 |
| MS.gene050116 | MS.gene60144 | 0.813452 | 2.54E-51 | -1.69E-46 |
| MS.gene050359 | MS.gene60144 | 0.859365 | 4.35E-63 | -1.69E-46 |
| MS.gene050361 | MS.gene60144 | 0.857978 | 1.13E-62 | -1.69E-46 |
| MS.gene050386 | MS.gene60144 | 0.80134 | 9.43E-49 | -1.69E-46 |
| MS.gene050551 | MS.gene60144 | 0.834454 | 2.97E-56 | -1.69E-46 |
| MS.gene050556 | MS.gene60144 | 0.83399 | 3.88E-56 | -1.69E-46 |
| MS.gene050629 | MS.gene60144 | -0.814321 | 1.64E-51 | -1.69E-46 |
| MS.gene050646 | MS.gene60144 | 0.837935 | 3.88E-57 | -1.69E-46 |
| MS.gene050654 | MS.gene60144 | 0.822811 | 1.94E-53 | -1.69E-46 |
| MS.gene050762 | MS.gene60144 | 0.811919 | 5.50E-51 | -1.69E-46 |
| MS.gene050763 | MS.gene60144 | 0.80893 | 2.43E-50 | -1.69E-46 |
| MS.gene050838 | MS.gene60144 | 0.839868 | 1.22E-57 | -1.69E-46 |
| MS.gene051102 | MS.gene60144 | 0.85268 | 3.94E-61 | -1.69E-46 |
| MS.gene051233 | MS.gene60144 | 0.809255 | 2.07E-50 | -1.69E-46 |
| MS.gene051343 | MS.gene60144 | 0.825328 | 4.98E-54 | -1.69E-46 |
| MS.gene051381 | MS.gene60144 | 0.821916 | 3.13E-53 | -1.69E-46 |
| MS.gene051642 | MS.gene60144 | 0.800399 | 1.47E-48 | -1.69E-46 |
| MS.gene051676 | MS.gene60144 | 0.802148 | 6.44E-49 | -1.69E-46 |
| MS.gene051809 | MS.gene60144 | 0.804155 | 2.47E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene60144 | 0.800082 | 1.70E-48 | -1.69E-46 |
| MS.gene051852 | MS.gene60144 | 0.815668 | 8.21E-52 | -1.69E-46 |
| MS.gene051856 | MS.gene60144 | 0.807185 | 5.72E-50 | -1.69E-46 |
| MS.gene052242 | MS.gene60144 | -0.859391 | 4.27E-63 | -1.69E-46 |
| MS.gene052307 | MS.gene60144 | 0.854023 | 1.62E-61 | -1.69E-46 |
| MS.gene052335 | MS.gene60144 | 0.812615 | 3.88E-51 | -1.69E-46 |
| MS.gene052342 | MS.gene60144 | 0.819924 | 9.00E-53 | -1.69E-46 |
| MS.gene052397 | MS.gene60144 | 0.816548 | 5.23E-52 | -1.69E-46 |
| MS.gene052463 | MS.gene60144 | 0.808145 | 3.58E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene60144 | 0.802169 | 6.37E-49 | -1.69E-46 |
| MS.gene052609 | MS.gene60144 | 0.876501 | 1.33E-68 | -1.69E-46 |
| MS.gene052776 | MS.gene60144 | 0.815063 | 1.12E-51 | -1.69E-46 |
| MS.gene052932 | MS.gene60144 | 0.817893 | 2.60E-52 | -1.69E-46 |
| MS.gene052994 | MS.gene60144 | 0.849483 | 3.14E-60 | -1.69E-46 |
| MS.gene053058 | MS.gene60144 | 0.803888 | 2.81E-49 | -1.69E-46 |
| MS.gene053079 | MS.gene60144 | 0.814017 | 1.91E-51 | -1.69E-46 |
| MS.gene053143 | MS.gene60144 | 0.849529 | 3.05E-60 | -1.69E-46 |
| MS.gene053167 | MS.gene60144 | 0.820655 | 6.12E-53 | -1.69E-46 |
| MS.gene054379 | MS.gene60144 | 0.804072 | 2.57E-49 | -1.69E-46 |
| MS.gene054824 | MS.gene60144 | 0.824519 | 7.73E-54 | -1.69E-46 |
| MS.gene055311 | MS.gene60144 | 0.866522 | 2.68E-65 | -1.69E-46 |
| MS.gene055313 | MS.gene60144 | 0.847223 | 1.32E-59 | -1.69E-46 |
| MS.gene055314 | MS.gene60144 | 0.834492 | 2.90E-56 | -1.69E-46 |
| MS.gene055469 | MS.gene60144 | 0.857188 | 1.93E-62 | -1.69E-46 |
| MS.gene055513 | MS.gene60144 | 0.833695 | 4.60E-56 | -1.69E-46 |
| MS.gene055864 | MS.gene60144 | 0.821129 | 4.76E-53 | -1.69E-46 |
| MS.gene056045 | MS.gene60144 | 0.840642 | 7.69E-58 | -1.69E-46 |
| MS.gene056097 | MS.gene60144 | 0.82364 | 1.24E-53 | -1.69E-46 |
| MS.gene056181 | MS.gene60144 | 0.851541 | 8.30E-61 | -1.69E-46 |
| MS.gene056308 | MS.gene60144 | 0.822687 | 2.07E-53 | -1.69E-46 |
| MS.gene056390 | MS.gene60144 | 0.824522 | 7.71E-54 | -1.69E-46 |
| MS.gene056507 | MS.gene60144 | 0.820178 | 7.87E-53 | -1.69E-46 |
| MS.gene056578 | MS.gene60144 | 0.803039 | 4.22E-49 | -1.69E-46 |
| MS.gene056787 | MS.gene60144 | 0.805461 | 1.32E-49 | -1.69E-46 |
| MS.gene057890 | MS.gene60144 | -0.800239 | 1.58E-48 | -1.69E-46 |
| MS.gene058054 | MS.gene60144 | 0.804773 | 1.84E-49 | -1.69E-46 |
| MS.gene058289 | MS.gene60144 | 0.803846 | 2.87E-49 | -1.69E-46 |
| MS.gene058292 | MS.gene60144 | 0.804082 | 2.56E-49 | -1.69E-46 |
| MS.gene058638 | MS.gene60144 | 0.89207 | 2.23E-74 | -1.69E-46 |
| MS.gene058639 | MS.gene60144 | 0.886846 | 2.40E-72 | -1.69E-46 |
| MS.gene058646 | MS.gene60144 | 0.807711 | 4.42E-50 | -1.69E-46 |
| MS.gene058647 | MS.gene60144 | 0.826822 | 2.20E-54 | -1.69E-46 |
| MS.gene058758 | MS.gene60144 | 0.837498 | 5.02E-57 | -1.69E-46 |
| MS.gene058852 | MS.gene60144 | 0.806819 | 6.84E-50 | -1.69E-46 |
| MS.gene059007 | MS.gene60144 | 0.837273 | 5.73E-57 | -1.69E-46 |
| MS.gene059170 | MS.gene60144 | 0.827834 | 1.26E-54 | -1.69E-46 |
| MS.gene059171 | MS.gene60144 | 0.851156 | 1.07E-60 | -1.69E-46 |
| MS.gene059439 | MS.gene60144 | 0.814976 | 1.17E-51 | -1.69E-46 |
| MS.gene059713 | MS.gene60144 | 0.822891 | 1.86E-53 | -1.69E-46 |
| MS.gene059725 | MS.gene60144 | 0.812407 | 4.31E-51 | -1.69E-46 |
| MS.gene059965 | MS.gene60144 | 0.80792 | 3.99E-50 | -1.69E-46 |
| MS.gene06010 | MS.gene60144 | 0.817853 | 2.66E-52 | -1.69E-46 |
| MS.gene060296 | MS.gene60144 | 0.834097 | 3.65E-56 | -1.69E-46 |
| MS.gene060380 | MS.gene60144 | 0.821696 | 3.52E-53 | -1.69E-46 |
| MS.gene060400 | MS.gene60144 | 0.842725 | 2.17E-58 | -1.69E-46 |
| MS.gene060563 | MS.gene60144 | 0.84788 | 8.74E-60 | -1.69E-46 |
| MS.gene060637 | MS.gene60144 | 0.817085 | 3.96E-52 | -1.69E-46 |
| MS.gene060869 | MS.gene60144 | 0.831878 | 1.30E-55 | -1.69E-46 |
| MS.gene060870 | MS.gene60144 | 0.81953 | 1.11E-52 | -1.69E-46 |
| MS.gene060932 | MS.gene60144 | 0.809909 | 1.50E-50 | -1.69E-46 |
| MS.gene061006 | MS.gene60144 | 0.86882 | 4.91E-66 | -1.69E-46 |
| MS.gene061100 | MS.gene60144 | 0.827946 | 1.18E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTAATGAAGAGCTATTTA+AGG | 0.167784 | 8.3:+12323675 | MS.gene60144:CDS |
| AATTACACAGTTGTTGACTT+TGG | 0.427244 | 8.3:+12323699 | MS.gene60144:CDS |
| TTGAGGAAATGGTGACCAAT+GGG | 0.454619 | 8.3:+12323395 | MS.gene60144:CDS |
| GTGAAGGTTGGAGCAAGAAT+CGG | 0.457365 | 8.3:+12323348 | None:intergenic |
| GACCAATGGGGAGTGGGATG+AGG | 0.481423 | 8.3:+12323408 | MS.gene60144:CDS |
| CACCTCATCCCACTCCCCAT+TGG | 0.483789 | 8.3:-12323410 | None:intergenic |
| TGAAGGTTGGAGCAAGAATC+GGG | 0.485517 | 8.3:+12323349 | None:intergenic |
| AAATGGTGACCAATGGGGAG+TGG | 0.485634 | 8.3:+12323401 | MS.gene60144:CDS |
| CAATGGGGAGTGGGATGAGG+TGG | 0.522573 | 8.3:+12323411 | MS.gene60144:CDS |
| AATGGTGACCAATGGGGAGT+GGG | 0.538530 | 8.3:+12323402 | MS.gene60144:CDS |
| GCAGAAGTATCTCGAAGCGT+TGG | 0.550896 | 8.3:+12323507 | MS.gene60144:CDS |
| TTTGAGGAAATGGTGACCAA+TGG | 0.553280 | 8.3:+12323394 | MS.gene60144:CDS |
| GAGGTGGAGAAGTACTTGAC+TGG | 0.582546 | 8.3:+12323427 | MS.gene60144:CDS |
| GTATCGATTATCATCAACCT+TGG | 0.585642 | 8.3:-12323455 | None:intergenic |
| AGCTGTGGATATATTAGTGA+AGG | 0.593264 | 8.3:+12323633 | MS.gene60144:CDS |
| GGGAGATCGATCGAAAGCTG+TGG | 0.666639 | 8.3:+12323618 | MS.gene60144:CDS |
| TGAGGAAATGGTGACCAATG+GGG | 0.678616 | 8.3:+12323396 | MS.gene60144:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTTTAATGAAGAGCTATTTA+AGG | + | chr8.3:12323675-12323694 | MS.gene60144:CDS | 25.0% |
| !!! | TGTTGACTTTGGATAATTTT+AGG | + | chr8.3:12323710-12323729 | MS.gene60144:CDS | 25.0% |
| ! | TGAAGATTTTCTTCGAGATT+AGG | + | chr8.3:12323482-12323501 | MS.gene60144:CDS | 30.0% |
| !! | AATTACACAGTTGTTGACTT+TGG | + | chr8.3:12323699-12323718 | MS.gene60144:CDS | 30.0% |
| !! | CTTTAACATGAGGTATTTTG+AGG | + | chr8.3:12323378-12323397 | MS.gene60144:CDS | 30.0% |
| AAACGAGGAAAAAATGTTGC+AGG | + | chr8.3:12323596-12323615 | MS.gene60144:intron | 35.0% | |
| AACGAGGAAAAAATGTTGCA+GGG | + | chr8.3:12323597-12323616 | MS.gene60144:intron | 35.0% | |
| AGCTGTGGATATATTAGTGA+AGG | + | chr8.3:12323633-12323652 | MS.gene60144:CDS | 35.0% | |
| GGTTTACTTGTTCTGATGTT+TGG | + | chr8.3:12323537-12323556 | MS.gene60144:intron | 35.0% | |
| ! | CGGGATTTTTCTTTAACATG+AGG | + | chr8.3:12323368-12323387 | MS.gene60144:CDS | 35.0% |
| !! | CATGAGGTATTTTGAGGAAA+TGG | + | chr8.3:12323384-12323403 | MS.gene60144:CDS | 35.0% |
| !! | GTATCGATTATCATCAACCT+TGG | - | chr8.3:12323458-12323477 | None:intergenic | 35.0% |
| ACGAGGAAAAAATGTTGCAG+GGG | + | chr8.3:12323598-12323617 | MS.gene60144:intron | 40.0% | |
| GATTGCTCTGATGTTAAACG+AGG | + | chr8.3:12323581-12323600 | MS.gene60144:intron | 40.0% | |
| TTGAGGAAATGGTGACCAAT+GGG | + | chr8.3:12323395-12323414 | MS.gene60144:CDS | 40.0% | |
| TTTGAGGAAATGGTGACCAA+TGG | + | chr8.3:12323394-12323413 | MS.gene60144:CDS | 40.0% | |
| !!! | GTACTTGACTGGTTTTACCA+AGG | + | chr8.3:12323438-12323457 | MS.gene60144:CDS | 40.0% |
| TGAGGAAATGGTGACCAATG+GGG | + | chr8.3:12323396-12323415 | MS.gene60144:CDS | 45.0% | |
| !! | TCTCGAAGCGTTGGATAAGT+AGG | + | chr8.3:12323516-12323535 | MS.gene60144:intron | 45.0% |
| AAATGGTGACCAATGGGGAG+TGG | + | chr8.3:12323401-12323420 | MS.gene60144:CDS | 50.0% | |
| AATGGTGACCAATGGGGAGT+GGG | + | chr8.3:12323402-12323421 | MS.gene60144:CDS | 50.0% | |
| !! | GAGGTGGAGAAGTACTTGAC+TGG | + | chr8.3:12323427-12323446 | MS.gene60144:CDS | 50.0% |
| !! | GCAGAAGTATCTCGAAGCGT+TGG | + | chr8.3:12323507-12323526 | MS.gene60144:CDS | 50.0% |
| GGGAGATCGATCGAAAGCTG+TGG | + | chr8.3:12323618-12323637 | MS.gene60144:CDS | 55.0% | |
| CAATGGGGAGTGGGATGAGG+TGG | + | chr8.3:12323411-12323430 | MS.gene60144:CDS | 60.0% | |
| CACCTCATCCCACTCCCCAT+TGG | - | chr8.3:12323413-12323432 | None:intergenic | 60.0% | |
| GACCAATGGGGAGTGGGATG+AGG | + | chr8.3:12323408-12323427 | MS.gene60144:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 12323354 | 12323731 | 12323354 | ID=MS.gene60144 |
| chr8.3 | mRNA | 12323354 | 12323731 | 12323354 | ID=MS.gene60144.t1;Parent=MS.gene60144 |
| chr8.3 | exon | 12323354 | 12323533 | 12323354 | ID=MS.gene60144.t1.exon1;Parent=MS.gene60144.t1 |
| chr8.3 | CDS | 12323354 | 12323533 | 12323354 | ID=cds.MS.gene60144.t1;Parent=MS.gene60144.t1 |
| chr8.3 | exon | 12323618 | 12323731 | 12323618 | ID=MS.gene60144.t1.exon2;Parent=MS.gene60144.t1 |
| chr8.3 | CDS | 12323618 | 12323731 | 12323618 | ID=cds.MS.gene60144.t1;Parent=MS.gene60144.t1 |
| Gene Sequence |
| Protein sequence |
