Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48283.t1 | AFK38340.1 | 92 | 50 | 4 | 0 | 1 | 50 | 1 | 50 | 3.00E-16 | 93.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48283.t1 | Q93Y35 | 50.0 | 50 | 25 | 0 | 1 | 50 | 1 | 50 | 1.1e-06 | 53.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48283.t1 | I3SS01 | 92.0 | 50 | 4 | 0 | 1 | 50 | 1 | 50 | 2.2e-16 | 93.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene48283 | 0.829504 | 4.96E-55 | -1.69E-46 |
| MS.gene04915 | MS.gene48283 | 0.815821 | 7.60E-52 | -1.69E-46 |
| MS.gene04917 | MS.gene48283 | 0.815301 | 9.92E-52 | -1.69E-46 |
| MS.gene049801 | MS.gene48283 | 0.802872 | 4.57E-49 | -1.69E-46 |
| MS.gene04984 | MS.gene48283 | 0.829699 | 4.45E-55 | -1.69E-46 |
| MS.gene049887 | MS.gene48283 | 0.815217 | 1.04E-51 | -1.69E-46 |
| MS.gene050084 | MS.gene48283 | 0.883963 | 2.87E-71 | -1.69E-46 |
| MS.gene050085 | MS.gene48283 | 0.88966 | 1.99E-73 | -1.69E-46 |
| MS.gene050114 | MS.gene48283 | 0.840291 | 9.50E-58 | -1.69E-46 |
| MS.gene050116 | MS.gene48283 | 0.838615 | 2.59E-57 | -1.69E-46 |
| MS.gene050224 | MS.gene48283 | 0.810661 | 1.03E-50 | -1.69E-46 |
| MS.gene050317 | MS.gene48283 | 0.82743 | 1.57E-54 | -1.69E-46 |
| MS.gene050359 | MS.gene48283 | 0.863267 | 2.81E-64 | -1.69E-46 |
| MS.gene050361 | MS.gene48283 | 0.8226 | 2.17E-53 | -1.69E-46 |
| MS.gene050386 | MS.gene48283 | 0.810113 | 1.35E-50 | -1.69E-46 |
| MS.gene050439 | MS.gene48283 | 0.814878 | 1.23E-51 | -1.69E-46 |
| MS.gene050551 | MS.gene48283 | 0.835178 | 1.95E-56 | -1.69E-46 |
| MS.gene050556 | MS.gene48283 | 0.8097 | 1.66E-50 | -1.69E-46 |
| MS.gene050646 | MS.gene48283 | 0.835987 | 1.22E-56 | -1.69E-46 |
| MS.gene050654 | MS.gene48283 | 0.843922 | 1.04E-58 | -1.69E-46 |
| MS.gene050689 | MS.gene48283 | 0.802456 | 5.56E-49 | -1.69E-46 |
| MS.gene050762 | MS.gene48283 | 0.800498 | 1.40E-48 | -1.69E-46 |
| MS.gene050838 | MS.gene48283 | 0.857624 | 1.44E-62 | -1.69E-46 |
| MS.gene050941 | MS.gene48283 | 0.824761 | 6.77E-54 | -1.69E-46 |
| MS.gene051003 | MS.gene48283 | 0.830162 | 3.43E-55 | -1.69E-46 |
| MS.gene051102 | MS.gene48283 | 0.862969 | 3.48E-64 | -1.69E-46 |
| MS.gene051184 | MS.gene48283 | 0.847831 | 9.01E-60 | -1.69E-46 |
| MS.gene051233 | MS.gene48283 | 0.814943 | 1.19E-51 | -1.69E-46 |
| MS.gene051343 | MS.gene48283 | 0.850799 | 1.34E-60 | -1.69E-46 |
| MS.gene051381 | MS.gene48283 | 0.839585 | 1.45E-57 | -1.69E-46 |
| MS.gene051642 | MS.gene48283 | 0.829462 | 5.08E-55 | -1.69E-46 |
| MS.gene051676 | MS.gene48283 | 0.81168 | 6.20E-51 | -1.69E-46 |
| MS.gene051757 | MS.gene48283 | 0.821401 | 4.12E-53 | -1.69E-46 |
| MS.gene051809 | MS.gene48283 | 0.865004 | 8.08E-65 | -1.69E-46 |
| MS.gene051831 | MS.gene48283 | 0.838074 | 3.57E-57 | -1.69E-46 |
| MS.gene051852 | MS.gene48283 | 0.830425 | 2.96E-55 | -1.69E-46 |
| MS.gene051975 | MS.gene48283 | 0.809032 | 2.31E-50 | -1.69E-46 |
| MS.gene052072 | MS.gene48283 | 0.80127 | 9.74E-49 | -1.69E-46 |
| MS.gene052242 | MS.gene48283 | -0.846351 | 2.29E-59 | -1.69E-46 |
| MS.gene052307 | MS.gene48283 | 0.84968 | 2.77E-60 | -1.69E-46 |
| MS.gene052342 | MS.gene48283 | 0.82273 | 2.03E-53 | -1.69E-46 |
| MS.gene052397 | MS.gene48283 | 0.834864 | 2.34E-56 | -1.69E-46 |
| MS.gene052434 | MS.gene48283 | 0.829898 | 3.98E-55 | -1.69E-46 |
| MS.gene052609 | MS.gene48283 | 0.867938 | 9.46E-66 | -1.69E-46 |
| MS.gene052994 | MS.gene48283 | 0.824874 | 6.37E-54 | -1.69E-46 |
| MS.gene053058 | MS.gene48283 | 0.846868 | 1.66E-59 | -1.69E-46 |
| MS.gene053143 | MS.gene48283 | 0.829042 | 6.42E-55 | -1.69E-46 |
| MS.gene05315 | MS.gene48283 | 0.823744 | 1.17E-53 | -1.69E-46 |
| MS.gene053167 | MS.gene48283 | 0.831929 | 1.26E-55 | -1.69E-46 |
| MS.gene053381 | MS.gene48283 | 0.819427 | 1.17E-52 | -1.69E-46 |
| MS.gene053564 | MS.gene48283 | 0.807408 | 5.13E-50 | -1.69E-46 |
| MS.gene053726 | MS.gene48283 | 0.800707 | 1.27E-48 | -1.69E-46 |
| MS.gene054558 | MS.gene48283 | 0.808354 | 3.23E-50 | -1.69E-46 |
| MS.gene054823 | MS.gene48283 | 0.822604 | 2.17E-53 | -1.69E-46 |
| MS.gene055311 | MS.gene48283 | 0.857383 | 1.69E-62 | -1.69E-46 |
| MS.gene055313 | MS.gene48283 | 0.832375 | 9.80E-56 | -1.69E-46 |
| MS.gene055314 | MS.gene48283 | 0.82573 | 4.00E-54 | -1.69E-46 |
| MS.gene055441 | MS.gene48283 | 0.802046 | 6.76E-49 | -1.69E-46 |
| MS.gene055469 | MS.gene48283 | 0.833045 | 6.68E-56 | -1.69E-46 |
| MS.gene055513 | MS.gene48283 | 0.825323 | 4.99E-54 | -1.69E-46 |
| MS.gene055864 | MS.gene48283 | 0.832017 | 1.20E-55 | -1.69E-46 |
| MS.gene056045 | MS.gene48283 | 0.810161 | 1.32E-50 | -1.69E-46 |
| MS.gene056097 | MS.gene48283 | 0.85487 | 9.22E-62 | -1.69E-46 |
| MS.gene056181 | MS.gene48283 | 0.85646 | 3.17E-62 | -1.69E-46 |
| MS.gene056390 | MS.gene48283 | 0.818346 | 2.06E-52 | -1.69E-46 |
| MS.gene056787 | MS.gene48283 | 0.853639 | 2.09E-61 | -1.69E-46 |
| MS.gene056831 | MS.gene48283 | 0.813435 | 2.56E-51 | -1.69E-46 |
| MS.gene056849 | MS.gene48283 | 0.821685 | 3.54E-53 | -1.69E-46 |
| MS.gene056992 | MS.gene48283 | 0.820973 | 5.17E-53 | -1.69E-46 |
| MS.gene057131 | MS.gene48283 | 0.800429 | 1.45E-48 | -1.69E-46 |
| MS.gene057360 | MS.gene48283 | 0.808274 | 3.36E-50 | -1.69E-46 |
| MS.gene057890 | MS.gene48283 | -0.811015 | 8.65E-51 | -1.69E-46 |
| MS.gene058054 | MS.gene48283 | 0.844889 | 5.72E-59 | -1.69E-46 |
| MS.gene058055 | MS.gene48283 | 0.809093 | 2.24E-50 | -1.69E-46 |
| MS.gene058278 | MS.gene48283 | 0.80039 | 1.47E-48 | -1.69E-46 |
| MS.gene058292 | MS.gene48283 | 0.847871 | 8.79E-60 | -1.69E-46 |
| MS.gene058638 | MS.gene48283 | 0.874062 | 9.05E-68 | -1.69E-46 |
| MS.gene058639 | MS.gene48283 | 0.871318 | 7.49E-67 | -1.69E-46 |
| MS.gene058646 | MS.gene48283 | 0.826449 | 2.70E-54 | -1.69E-46 |
| MS.gene058654 | MS.gene48283 | 0.820726 | 5.89E-53 | -1.69E-46 |
| MS.gene058758 | MS.gene48283 | 0.87446 | 6.64E-68 | -1.69E-46 |
| MS.gene05880 | MS.gene48283 | 0.808965 | 2.39E-50 | -1.69E-46 |
| MS.gene058852 | MS.gene48283 | 0.801991 | 6.93E-49 | -1.69E-46 |
| MS.gene059007 | MS.gene48283 | 0.888683 | 4.75E-73 | -1.69E-46 |
| MS.gene059050 | MS.gene48283 | 0.821128 | 4.76E-53 | -1.69E-46 |
| MS.gene059146 | MS.gene48283 | 0.809661 | 1.69E-50 | -1.69E-46 |
| MS.gene059170 | MS.gene48283 | 0.828679 | 7.86E-55 | -1.69E-46 |
| MS.gene059171 | MS.gene48283 | 0.850546 | 1.58E-60 | -1.69E-46 |
| MS.gene059327 | MS.gene48283 | 0.804357 | 2.25E-49 | -1.69E-46 |
| MS.gene059360 | MS.gene48283 | 0.84897 | 4.37E-60 | -1.69E-46 |
| MS.gene059713 | MS.gene48283 | 0.818486 | 1.91E-52 | -1.69E-46 |
| MS.gene06010 | MS.gene48283 | 0.872416 | 3.24E-67 | -1.69E-46 |
| MS.gene060259 | MS.gene48283 | 0.800432 | 1.45E-48 | -1.69E-46 |
| MS.gene060296 | MS.gene48283 | 0.84514 | 4.89E-59 | -1.69E-46 |
| MS.gene060380 | MS.gene48283 | 0.841322 | 5.10E-58 | -1.69E-46 |
| MS.gene060400 | MS.gene48283 | 0.82763 | 1.41E-54 | -1.69E-46 |
| MS.gene060465 | MS.gene48283 | 0.80623 | 9.10E-50 | -1.69E-46 |
| MS.gene060563 | MS.gene48283 | 0.848848 | 4.72E-60 | -1.69E-46 |
| MS.gene060637 | MS.gene48283 | 0.810376 | 1.19E-50 | -1.69E-46 |
| MS.gene060869 | MS.gene48283 | 0.835157 | 1.98E-56 | -1.69E-46 |
| MS.gene060870 | MS.gene48283 | 0.845791 | 3.26E-59 | -1.69E-46 |
| MS.gene060932 | MS.gene48283 | 0.800964 | 1.13E-48 | -1.69E-46 |
| MS.gene060933 | MS.gene48283 | 0.811783 | 5.89E-51 | -1.69E-46 |
| MS.gene061006 | MS.gene48283 | 0.878882 | 1.96E-69 | -1.69E-46 |
| MS.gene061100 | MS.gene48283 | 0.826115 | 3.24E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48283.t1 | MTR_4g104080 | 92.000 | 50 | 4 | 0 | 1 | 50 | 1 | 50 | 1.23e-25 | 96.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48283.t1 | AT4G24820 | 50.000 | 50 | 25 | 0 | 1 | 50 | 1 | 50 | 3.80e-11 | 56.2 |
| MS.gene48283.t1 | AT4G24820 | 50.000 | 50 | 25 | 0 | 1 | 50 | 1 | 50 | 3.80e-11 | 56.2 |
Find 9 sgRNAs with CRISPR-Local
Find 8 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGAACATCTTGTTGTTTG+AGG | 0.383548 | 8.1:-20336590 | None:intergenic |
| GGGTTTACGGAGAGAGAAGA+GGG | 0.436554 | 8.1:-20336675 | None:intergenic |
| AGGGTTTACGGAGAGAGAAG+AGG | 0.479041 | 8.1:-20336676 | None:intergenic |
| TTTGAGGAGGAAAAGCTTGT+CGG | 0.496982 | 8.1:-20336574 | None:intergenic |
| TTCAATTTCGTCAAGGAACA+TGG | 0.512182 | 8.1:+20336645 | MS.gene48283:CDS |
| ATCGCGTCAATGGCAAATGA+AGG | 0.573429 | 8.1:+20336519 | None:intergenic |
| GAACATCTTGTTGTTTGAGG+AGG | 0.589500 | 8.1:-20336587 | None:intergenic |
| GGAGGAAAAGCTTGTCGGCG+AGG | 0.605693 | 8.1:-20336569 | None:intergenic |
| TTGTCGGCGAGGACAAGTTG+TGG | 0.627071 | 8.1:-20336558 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGATGTTTTCAATTTCGTCA+AGG | + | chr8.1:20336638-20336657 | MS.gene48283:CDS | 30.0% |
| TTCAATTTCGTCAAGGAACA+TGG | + | chr8.1:20336645-20336664 | MS.gene48283:CDS | 35.0% | |
| !! | CTTGAACATCTTGTTGTTTG+AGG | - | chr8.1:20336593-20336612 | None:intergenic | 35.0% |
| TTCTTCACCTTCATTTGCCA+TGG | - | chr8.1:20336529-20336548 | None:intergenic | 40.0% | |
| TTTGAGGAGGAAAAGCTTGT+CGG | - | chr8.1:20336577-20336596 | None:intergenic | 40.0% | |
| !! | GAACATCTTGTTGTTTGAGG+AGG | - | chr8.1:20336590-20336609 | None:intergenic | 40.0% |
| TTGTCGGCGAGGACAAGTTG+TGG | - | chr8.1:20336561-20336580 | None:intergenic | 55.0% | |
| GGAGGAAAAGCTTGTCGGCG+AGG | - | chr8.1:20336572-20336591 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 20336528 | 20336689 | 20336528 | ID=MS.gene48283 |
| chr8.1 | mRNA | 20336528 | 20336689 | 20336528 | ID=MS.gene48283.t1;Parent=MS.gene48283 |
| chr8.1 | exon | 20336528 | 20336689 | 20336528 | ID=MS.gene48283.t1.exon1;Parent=MS.gene48283.t1 |
| chr8.1 | CDS | 20336528 | 20336689 | 20336528 | ID=cds.MS.gene48283.t1;Parent=MS.gene48283.t1 |
| Gene Sequence |
| Protein sequence |
