Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052777.t1 | XP_003604587.1 | 99.8 | 447 | 1 | 0 | 1 | 447 | 1 | 447 | 1.00E-258 | 902.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052777.t1 | O64937 | 94.6 | 447 | 24 | 0 | 1 | 447 | 1 | 447 | 6.7e-251 | 867.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052777.t1 | G7JLV1 | 99.8 | 447 | 1 | 0 | 1 | 447 | 1 | 447 | 7.3e-259 | 902.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene052777 | -0.806529 | 7.87E-50 | -1.69E-46 |
| MS.gene052777 | MS.gene053103 | -0.800182 | 1.62E-48 | -1.69E-46 |
| MS.gene052777 | MS.gene053104 | -0.80066 | 1.30E-48 | -1.69E-46 |
| MS.gene052777 | MS.gene058082 | -0.80176 | 7.73E-49 | -1.69E-46 |
| MS.gene052777 | MS.gene059711 | -0.805332 | 1.40E-49 | -1.69E-46 |
| MS.gene052777 | MS.gene060804 | -0.80005 | 1.73E-48 | -1.69E-46 |
| MS.gene052777 | MS.gene060837 | -0.81626 | 6.06E-52 | -1.69E-46 |
| MS.gene052777 | MS.gene06142 | -0.805853 | 1.09E-49 | -1.69E-46 |
| MS.gene052777 | MS.gene062891 | -0.809341 | 1.99E-50 | -1.69E-46 |
| MS.gene052777 | MS.gene06475 | -0.813857 | 2.07E-51 | -1.69E-46 |
| MS.gene052777 | MS.gene09357 | -0.802354 | 5.84E-49 | -1.69E-46 |
| MS.gene052777 | MS.gene21413 | -0.809222 | 2.10E-50 | -1.69E-46 |
| MS.gene052777 | MS.gene22222 | -0.808117 | 3.63E-50 | -1.69E-46 |
| MS.gene052777 | MS.gene23621 | -0.808187 | 3.50E-50 | -1.69E-46 |
| MS.gene052777 | MS.gene33718 | 0.965888 | 3.43E-125 | -1.69E-46 |
| MS.gene052777 | MS.gene44005 | -0.800428 | 1.45E-48 | -1.69E-46 |
| MS.gene052777 | MS.gene50731 | -0.805609 | 1.23E-49 | -1.69E-46 |
| MS.gene052777 | MS.gene55026 | -0.807676 | 4.50E-50 | -1.69E-46 |
| MS.gene052777 | MS.gene58120 | -0.832237 | 1.06E-55 | -1.69E-46 |
| MS.gene052777 | MS.gene60238 | -0.808426 | 3.11E-50 | -1.69E-46 |
| MS.gene052777 | MS.gene61160 | -0.817849 | 2.66E-52 | -1.69E-46 |
| MS.gene052777 | MS.gene61632 | -0.806176 | 9.34E-50 | -1.69E-46 |
| MS.gene052777 | MS.gene62087 | -0.808262 | 3.38E-50 | -1.69E-46 |
| MS.gene052777 | MS.gene63225 | -0.812489 | 4.13E-51 | -1.69E-46 |
| MS.gene052777 | MS.gene64678 | -0.812662 | 3.79E-51 | -1.69E-46 |
| MS.gene052777 | MS.gene66800 | -0.829598 | 4.71E-55 | -1.69E-46 |
| MS.gene052777 | MS.gene68423 | -0.808658 | 2.78E-50 | -1.69E-46 |
| MS.gene052777 | MS.gene72905 | -0.800536 | 1.38E-48 | -1.69E-46 |
| MS.gene052777 | MS.gene75297 | -0.803628 | 3.18E-49 | -1.69E-46 |
| MS.gene052777 | MS.gene87404 | -0.818535 | 1.86E-52 | -1.69E-46 |
| MS.gene052777 | MS.gene91570 | -0.813383 | 2.63E-51 | -1.69E-46 |
| MS.gene052777 | MS.gene93388 | 0.981722 | 2.76E-153 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052777.t1 | MTR_4g014810 | 99.776 | 447 | 1 | 0 | 1 | 447 | 1 | 447 | 0.0 | 919 |
| MS.gene052777.t1 | MTR_1g495705 | 96.429 | 448 | 15 | 1 | 1 | 447 | 1 | 448 | 0.0 | 891 |
| MS.gene052777.t1 | MTR_1g095650 | 96.429 | 448 | 15 | 1 | 1 | 447 | 1 | 448 | 0.0 | 891 |
| MS.gene052777.t1 | MTR_1g095703 | 96.205 | 448 | 16 | 1 | 1 | 447 | 1 | 448 | 0.0 | 890 |
| MS.gene052777.t1 | MTR_6g021800 | 96.083 | 434 | 17 | 0 | 1 | 434 | 1 | 434 | 0.0 | 874 |
| MS.gene052777.t1 | MTR_6g021805 | 96.083 | 434 | 17 | 0 | 1 | 434 | 1 | 434 | 0.0 | 874 |
| MS.gene052777.t1 | MTR_1g013680 | 95.195 | 437 | 18 | 1 | 1 | 434 | 1 | 437 | 0.0 | 857 |
| MS.gene052777.t1 | MTR_1g085410 | 82.258 | 434 | 77 | 0 | 1 | 434 | 1 | 434 | 0.0 | 743 |
| MS.gene052777.t1 | MTR_1g101870 | 80.963 | 436 | 78 | 3 | 1 | 436 | 1 | 431 | 0.0 | 731 |
| MS.gene052777.t1 | MTR_1g101870 | 80.963 | 436 | 78 | 3 | 1 | 436 | 1 | 431 | 0.0 | 728 |
| MS.gene052777.t1 | MTR_1g024175 | 78.802 | 434 | 92 | 0 | 1 | 434 | 1 | 434 | 0.0 | 726 |
| MS.gene052777.t1 | MTR_8g014590 | 79.065 | 449 | 91 | 2 | 1 | 446 | 1 | 449 | 0.0 | 717 |
| MS.gene052777.t1 | MTR_1g095650 | 95.389 | 347 | 15 | 1 | 102 | 447 | 1 | 347 | 0.0 | 682 |
| MS.gene052777.t1 | MTR_2g082730 | 73.364 | 428 | 97 | 2 | 1 | 426 | 1 | 413 | 0.0 | 647 |
| MS.gene052777.t1 | MTR_5g037350 | 67.377 | 469 | 116 | 1 | 3 | 434 | 13 | 481 | 0.0 | 634 |
| MS.gene052777.t1 | MTR_1g089520 | 70.930 | 430 | 107 | 3 | 4 | 432 | 5 | 417 | 0.0 | 625 |
| MS.gene052777.t1 | MTR_2g082665 | 68.224 | 428 | 93 | 2 | 1 | 426 | 1 | 387 | 0.0 | 590 |
| MS.gene052777.t1 | MTR_2g037420 | 64.159 | 452 | 116 | 4 | 1 | 447 | 1 | 411 | 0.0 | 566 |
| MS.gene052777.t1 | MTR_8g013910 | 64.286 | 448 | 125 | 5 | 1 | 432 | 1 | 429 | 0.0 | 555 |
| MS.gene052777.t1 | MTR_2g080210 | 63.453 | 446 | 121 | 5 | 1 | 443 | 1 | 407 | 0.0 | 551 |
| MS.gene052777.t1 | MTR_8g008740 | 78.154 | 325 | 70 | 1 | 1 | 324 | 1 | 325 | 0.0 | 540 |
| MS.gene052777.t1 | MTR_1g112460 | 64.765 | 298 | 63 | 4 | 155 | 446 | 1 | 262 | 6.55e-124 | 361 |
| MS.gene052777.t1 | MTR_1g495735 | 74.627 | 201 | 41 | 1 | 240 | 430 | 106 | 306 | 2.31e-99 | 300 |
| MS.gene052777.t1 | MTR_1g495735 | 71.698 | 53 | 15 | 0 | 21 | 73 | 2 | 54 | 4.76e-17 | 82.0 |
| MS.gene052777.t1 | MTR_6g080420 | 38.173 | 427 | 256 | 5 | 5 | 426 | 89 | 512 | 1.39e-98 | 305 |
| MS.gene052777.t1 | MTR_8g106700 | 37.939 | 427 | 255 | 6 | 4 | 424 | 43 | 465 | 9.44e-95 | 293 |
| MS.gene052777.t1 | MTR_8g106700 | 37.939 | 427 | 255 | 6 | 4 | 424 | 49 | 471 | 1.17e-94 | 293 |
| MS.gene052777.t1 | MTR_8g106700 | 37.939 | 427 | 255 | 6 | 4 | 424 | 70 | 492 | 3.41e-94 | 293 |
| MS.gene052777.t1 | MTR_8g087950 | 36.000 | 425 | 264 | 5 | 7 | 426 | 275 | 696 | 1.71e-90 | 289 |
| MS.gene052777.t1 | MTR_1g101880 | 87.013 | 154 | 20 | 0 | 1 | 154 | 1 | 154 | 6.54e-90 | 274 |
| MS.gene052777.t1 | MTR_8g087950 | 35.748 | 428 | 267 | 5 | 7 | 429 | 348 | 772 | 8.85e-90 | 289 |
| MS.gene052777.t1 | MTR_4g054970 | 56.612 | 242 | 76 | 4 | 193 | 431 | 50 | 265 | 4.45e-81 | 252 |
| MS.gene052777.t1 | MTR_4g054970 | 75.000 | 44 | 11 | 0 | 1 | 44 | 1 | 44 | 7.02e-13 | 69.3 |
| MS.gene052777.t1 | MTR_1g024165 | 81.429 | 140 | 26 | 0 | 6 | 145 | 10 | 149 | 1.11e-78 | 241 |
| MS.gene052777.t1 | MTR_8g014950 | 76.471 | 102 | 24 | 0 | 3 | 104 | 4 | 105 | 1.50e-47 | 159 |
| MS.gene052777.t1 | MTR_7g094460 | 30.137 | 438 | 246 | 14 | 3 | 429 | 59 | 447 | 6.75e-45 | 162 |
| MS.gene052777.t1 | MTR_2g021300 | 29.613 | 439 | 247 | 12 | 3 | 429 | 46 | 434 | 4.43e-43 | 157 |
| MS.gene052777.t1 | MTR_7g098190 | 53.293 | 167 | 52 | 5 | 56 | 220 | 10 | 152 | 1.16e-42 | 149 |
| MS.gene052777.t1 | MTR_3g076660 | 29.730 | 444 | 251 | 15 | 3 | 429 | 88 | 487 | 1.13e-39 | 149 |
| MS.gene052777.t1 | MTR_8g014960 | 73.239 | 71 | 19 | 0 | 213 | 283 | 2 | 72 | 3.11e-29 | 113 |
| MS.gene052777.t1 | MTR_3g097250 | 26.471 | 272 | 143 | 9 | 9 | 272 | 85 | 307 | 5.92e-13 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052777.t1 | AT5G60390 | 93.946 | 446 | 27 | 0 | 1 | 446 | 1 | 446 | 0.0 | 877 |
| MS.gene052777.t1 | AT5G60390 | 93.946 | 446 | 27 | 0 | 1 | 446 | 1 | 446 | 0.0 | 877 |
| MS.gene052777.t1 | AT5G60390 | 93.946 | 446 | 27 | 0 | 1 | 446 | 1 | 446 | 0.0 | 877 |
| MS.gene052777.t1 | AT1G07920 | 93.946 | 446 | 27 | 0 | 1 | 446 | 1 | 446 | 0.0 | 877 |
| MS.gene052777.t1 | AT1G07940 | 93.946 | 446 | 27 | 0 | 1 | 446 | 1 | 446 | 0.0 | 877 |
| MS.gene052777.t1 | AT1G07940 | 93.946 | 446 | 27 | 0 | 1 | 446 | 1 | 446 | 0.0 | 877 |
| MS.gene052777.t1 | AT1G07940 | 93.946 | 446 | 27 | 0 | 1 | 446 | 1 | 446 | 0.0 | 877 |
| MS.gene052777.t1 | AT1G07940 | 93.946 | 446 | 27 | 0 | 1 | 446 | 1 | 446 | 0.0 | 877 |
| MS.gene052777.t1 | AT1G07930 | 93.946 | 446 | 27 | 0 | 1 | 446 | 1 | 446 | 0.0 | 877 |
| MS.gene052777.t1 | AT1G07930 | 98.039 | 255 | 5 | 0 | 1 | 255 | 1 | 255 | 0.0 | 528 |
| MS.gene052777.t1 | AT1G07930 | 86.207 | 116 | 16 | 0 | 331 | 446 | 254 | 369 | 1.65e-64 | 212 |
| MS.gene052777.t1 | AT1G18070 | 37.176 | 425 | 259 | 5 | 5 | 424 | 99 | 520 | 2.79e-95 | 297 |
| MS.gene052777.t1 | AT1G18070 | 37.176 | 425 | 259 | 5 | 5 | 424 | 99 | 520 | 2.79e-95 | 297 |
| MS.gene052777.t1 | AT1G18070 | 36.468 | 436 | 258 | 6 | 5 | 424 | 99 | 531 | 3.24e-92 | 289 |
| MS.gene052777.t1 | AT5G10630 | 35.529 | 425 | 266 | 5 | 7 | 426 | 239 | 660 | 7.61e-91 | 289 |
| MS.gene052777.t1 | AT5G10630 | 35.529 | 425 | 266 | 5 | 7 | 426 | 240 | 661 | 7.79e-91 | 289 |
| MS.gene052777.t1 | AT5G10630 | 35.529 | 425 | 266 | 5 | 7 | 426 | 240 | 661 | 7.79e-91 | 289 |
| MS.gene052777.t1 | AT5G10630 | 35.529 | 425 | 266 | 5 | 7 | 426 | 310 | 731 | 2.86e-90 | 290 |
| MS.gene052777.t1 | AT5G10630 | 35.529 | 425 | 266 | 5 | 7 | 426 | 315 | 736 | 3.76e-90 | 289 |
| MS.gene052777.t1 | AT1G35550 | 75.962 | 104 | 25 | 0 | 335 | 438 | 1 | 104 | 2.33e-51 | 169 |
| MS.gene052777.t1 | AT4G20360 | 30.575 | 435 | 253 | 10 | 3 | 426 | 75 | 471 | 3.00e-44 | 161 |
| MS.gene052777.t1 | AT4G20360 | 30.575 | 435 | 253 | 10 | 3 | 426 | 75 | 471 | 3.00e-44 | 161 |
| MS.gene052777.t1 | AT4G02930 | 29.841 | 439 | 247 | 15 | 3 | 429 | 63 | 452 | 4.43e-43 | 157 |
| MS.gene052777.t1 | AT2G31060 | 25.000 | 264 | 160 | 8 | 9 | 263 | 61 | 295 | 4.32e-12 | 68.6 |
| MS.gene052777.t1 | AT5G39900 | 25.839 | 298 | 158 | 8 | 4 | 288 | 64 | 311 | 6.54e-12 | 68.2 |
| MS.gene052777.t1 | AT5G08650 | 26.518 | 313 | 172 | 13 | 9 | 311 | 88 | 352 | 1.16e-11 | 67.4 |
| MS.gene052777.t1 | AT5G08650 | 26.518 | 313 | 172 | 13 | 9 | 311 | 88 | 352 | 1.23e-11 | 67.4 |
| MS.gene052777.t1 | AT2G31060 | 24.615 | 260 | 162 | 7 | 9 | 263 | 61 | 291 | 5.55e-11 | 65.1 |
Find 133 sgRNAs with CRISPR-Local
Find 145 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCACGGTTCATTTCTTCT+TGG | 0.216911 | 4.4:-86101896 | None:intergenic |
| GCTCTCCTTGCTTTCACTCT+TGG | 0.289982 | 4.4:+86100874 | MS.gene052777:CDS |
| ATCTCCATTCTTCAGGAACT+TGG | 0.311336 | 4.4:-86101701 | None:intergenic |
| GTCTACAAGATTGGAGGAAT+TGG | 0.315206 | 4.4:+86101286 | MS.gene052777:CDS |
| AAGTTAGCAGCCTCCTTGGC+TGG | 0.316454 | 4.4:-86101526 | None:intergenic |
| CACATTAACATTGTTGTCAT+TGG | 0.329838 | 4.4:+86100484 | MS.gene052777:CDS |
| TTCATTAAGAACATGATTAC+TGG | 0.336870 | 4.4:+86100757 | MS.gene052777:CDS |
| TACCAGCATCTCCATTCTTC+AGG | 0.357413 | 4.4:-86101708 | None:intergenic |
| CTCCTTGGCTGGGTCATCCT+TGG | 0.371796 | 4.4:-86101515 | None:intergenic |
| GCAAAAGTCACCACCATTCC+AGG | 0.373840 | 4.4:-86101349 | None:intergenic |
| TCTCTCACTGAGGCACTTCC+TGG | 0.375823 | 4.4:+86101421 | MS.gene052777:CDS |
| ACTAAGATTGACAGGCGTTC+TGG | 0.379305 | 4.4:+86101658 | MS.gene052777:CDS |
| TGCGTCAAACTGTGGCTGTT+GGG | 0.385555 | 4.4:+86101815 | MS.gene052777:CDS |
| GTCATTGGCCATGTCGACTC+TGG | 0.395247 | 4.4:+86100499 | MS.gene052777:CDS |
| CACCACCATTCCAGGTTTCA+TGG | 0.400023 | 4.4:-86101341 | None:intergenic |
| ATTCCATTCGTTCCCATCTC+TGG | 0.404373 | 4.4:+86101127 | MS.gene052777:CDS |
| ATACCTGCTCTTGGAATACT+TGG | 0.419677 | 4.4:-86101044 | None:intergenic |
| AAGGACAATGGGTAAGGAAA+AGG | 0.424225 | 4.4:+86100459 | None:intergenic |
| ATGAATCACCCTGGCCAGAT+TGG | 0.425183 | 4.4:+86101568 | MS.gene052777:CDS |
| TTCACGGGTCTGACCATCCT+TGG | 0.425447 | 4.4:-86100848 | None:intergenic |
| CTCATCATTGACTCCACTAC+TGG | 0.425797 | 4.4:+86100805 | MS.gene052777:CDS |
| ATAGCCAACCTTCTTCATAT+AGG | 0.426065 | 4.4:-86101092 | None:intergenic |
| CTTAATGAAATCCCTGTGTC+CGG | 0.429384 | 4.4:-86100743 | None:intergenic |
| AATTTCATCATACCTGCTCT+TGG | 0.430266 | 4.4:-86101053 | None:intergenic |
| GGCTTGTCTGATGGCCTCTT+AGG | 0.432559 | 4.4:-86101241 | None:intergenic |
| CACCCTGGCCAGATTGGAAA+TGG | 0.432985 | 4.4:+86101574 | MS.gene052777:CDS |
| GCCCCTCCGACTACCACTTC+AGG | 0.433104 | 4.4:+86101261 | MS.gene052777:CDS |
| GAGGTGTGGCAGTCAAGCAC+AGG | 0.434332 | 4.4:-86101604 | None:intergenic |
| GGGCATAGCCATTTCCAATC+TGG | 0.448022 | 4.4:-86101582 | None:intergenic |
| AGCGTTCATTCAAGTATGCT+TGG | 0.450783 | 4.4:+86100617 | MS.gene052777:CDS |
| TGTGGCTGTTGGGGTTATCA+AGG | 0.455009 | 4.4:+86101825 | MS.gene052777:CDS |
| CTTCAGGATGTCTACAAGAT+TGG | 0.458119 | 4.4:+86101277 | MS.gene052777:CDS |
| GCGTTCATTCAAGTATGCTT+GGG | 0.461276 | 4.4:+86100618 | MS.gene052777:CDS |
| AGTCTCAACAACCATGGGCT+TGG | 0.462350 | 4.4:-86101749 | None:intergenic |
| CTCAACAACCATGGGCTTGG+TGG | 0.464097 | 4.4:-86101746 | None:intergenic |
| TTAATGAAATCCCTGTGTCC+GGG | 0.473933 | 4.4:-86100742 | None:intergenic |
| TAGCCATTTCCAATCTGGCC+AGG | 0.474048 | 4.4:-86101577 | None:intergenic |
| ATCAAATCAATGAGCCTAAG+AGG | 0.476814 | 4.4:+86101227 | MS.gene052777:CDS |
| TGTGAAGTTAGCAGCCTCCT+TGG | 0.480074 | 4.4:-86101530 | None:intergenic |
| TACAAATCTTGACTGGTACA+AGG | 0.490199 | 4.4:+86101180 | MS.gene052777:CDS |
| GAGCCCAAGTTCCTGAAGAA+TGG | 0.491587 | 4.4:+86101697 | MS.gene052777:CDS |
| AGTCGGAGGGGCTTGTCTGA+TGG | 0.496444 | 4.4:-86101250 | None:intergenic |
| ATCTCCCTCAAAACCAGAGA+TGG | 0.496573 | 4.4:-86101140 | None:intergenic |
| GATTGACAGGCGTTCTGGTA+AGG | 0.497183 | 4.4:+86101663 | MS.gene052777:CDS |
| AGCTTGTTACTAAGATTGAC+AGG | 0.504274 | 4.4:+86101650 | MS.gene052777:CDS |
| TGGGTCATCCTTGGTGTTTG+AGG | 0.504620 | 4.4:-86101506 | None:intergenic |
| CTTGGAATACTTGGGTGTAG+TGG | 0.514389 | 4.4:-86101035 | None:intergenic |
| TGAGAACAGCACAATCAGCT+TGG | 0.516820 | 4.4:-86100786 | None:intergenic |
| GAGAACAGCACAATCAGCTT+GGG | 0.517298 | 4.4:-86100785 | None:intergenic |
| TATCAAGGGTGTGGAGAAGA+AGG | 0.517386 | 4.4:+86101840 | MS.gene052777:CDS |
| ACTTCAGTTTGCAGTCCAGT+TGG | 0.517541 | 4.4:-86101373 | None:intergenic |
| CAAGAGCCTCAAGAAGTGTT+GGG | 0.518886 | 4.4:-86101204 | None:intergenic |
| TGTTAAGAATGTTTCTGTCA+AGG | 0.521267 | 4.4:+86101462 | MS.gene052777:CDS |
| CAGGATGTCTACAAGATTGG+AGG | 0.523217 | 4.4:+86101280 | MS.gene052777:CDS |
| TCTCCATTCTTCAGGAACTT+GGG | 0.527363 | 4.4:-86101700 | None:intergenic |
| TTGCTTTCACTCTTGGTGTG+AGG | 0.528420 | 4.4:+86100881 | MS.gene052777:CDS |
| TTCCTGAAGAATGGAGATGC+TGG | 0.528428 | 4.4:+86101706 | MS.gene052777:CDS |
| AGTTTCATCCTATATGAAGA+AGG | 0.530606 | 4.4:+86101084 | MS.gene052777:CDS |
| TCAAGAGCCTCAAGAAGTGT+TGG | 0.532468 | 4.4:-86101205 | None:intergenic |
| CTCAAGGCTGAGCGTGAAAG+AGG | 0.533500 | 4.4:+86100652 | MS.gene052777:CDS |
| AGTTAGCAGCCTCCTTGGCT+GGG | 0.535132 | 4.4:-86101525 | None:intergenic |
| TCATTGGCCATGTCGACTCT+GGG | 0.536467 | 4.4:+86100500 | MS.gene052777:CDS |
| AGCAAACTTGACAGCAATGT+GGG | 0.541075 | 4.4:-86101626 | None:intergenic |
| CCAGTCTCAACACGTCCGAC+AGG | 0.541163 | 4.4:-86101316 | None:intergenic |
| CCTGTCGGACGTGTTGAGAC+TGG | 0.541718 | 4.4:+86101316 | MS.gene052777:CDS |
| AGCTTCAAAACCACCAGTAG+TGG | 0.543721 | 4.4:-86100818 | None:intergenic |
| TTCTCTGAATATCCTCCACT+TGG | 0.543785 | 4.4:+86101772 | MS.gene052777:CDS |
| AGGTGTGGCAGTCAAGCACA+GGG | 0.544526 | 4.4:-86101603 | None:intergenic |
| TGCACTGTCATTGATGCCCC+CGG | 0.544718 | 4.4:+86100724 | MS.gene052777:CDS |
| TCATCCTATATGAAGAAGGT+TGG | 0.544820 | 4.4:+86101088 | MS.gene052777:CDS |
| CACTTGATCTACAAGCTTGG+AGG | 0.545798 | 4.4:+86100541 | MS.gene052777:CDS |
| ATCATTGACTCCACTACTGG+TGG | 0.550177 | 4.4:+86100808 | MS.gene052777:CDS |
| GGTCACTTGATCTACAAGCT+TGG | 0.555547 | 4.4:+86100538 | MS.gene052777:CDS |
| ATGCGTCAAACTGTGGCTGT+TGG | 0.557227 | 4.4:+86101814 | MS.gene052777:CDS |
| GAAGCTGGTATTTCCAAGGA+TGG | 0.557613 | 4.4:+86100835 | MS.gene052777:CDS |
| TCAACAACCATGGGCTTGGT+GGG | 0.557624 | 4.4:-86101745 | None:intergenic |
| AAGCAAGGAGAGCATGTTCA+CGG | 0.559179 | 4.4:-86100864 | None:intergenic |
| TGTTGGGGTTATCAAGGGTG+TGG | 0.563472 | 4.4:+86101831 | MS.gene052777:CDS |
| GCATATCTCTGACAGCAAAA+CGG | 0.563498 | 4.4:-86101795 | None:intergenic |
| ACAAATCTTGACTGGTACAA+GGG | 0.563768 | 4.4:+86101181 | MS.gene052777:CDS |
| GTGGCTGTTGGGGTTATCAA+GGG | 0.565604 | 4.4:+86101826 | MS.gene052777:CDS |
| TTAGTTATCTTAACAGTACC+AGG | 0.565646 | 4.4:-86101865 | None:intergenic |
| CAGGTATGATGAAATTGTGA+AGG | 0.569288 | 4.4:+86101060 | MS.gene052777:CDS |
| TCTGGGAAGTCAACTACCAC+TGG | 0.570179 | 4.4:+86100517 | MS.gene052777:CDS |
| TAATGAAATCCCTGTGTCCG+GGG | 0.570266 | 4.4:-86100741 | None:intergenic |
| CACCCAAGTATTCCAAGAGC+AGG | 0.570501 | 4.4:+86101041 | MS.gene052777:CDS |
| TTGGGTTCTTGACAAGCTCA+AGG | 0.572280 | 4.4:+86100636 | MS.gene052777:CDS |
| AGCAAGGAGAGCATGTTCAC+GGG | 0.572470 | 4.4:-86100863 | None:intergenic |
| ATCCTGAAGTGGTAGTCGGA+GGG | 0.576252 | 4.4:-86101263 | None:intergenic |
| GATGATTCCCACCAAGCCCA+TGG | 0.580619 | 4.4:+86101738 | MS.gene052777:CDS |
| AAACCAGAGATGGGAACGAA+TGG | 0.584939 | 4.4:-86101130 | None:intergenic |
| TCGACAAGCGTGTGATCGAG+AGG | 0.586165 | 4.4:+86100566 | MS.gene052777:CDS |
| GCGTCAAACTGTGGCTGTTG+GGG | 0.590275 | 4.4:+86101816 | MS.gene052777:CDS |
| GGCACTTCCTGGTGACAATG+TGG | 0.592116 | 4.4:+86101432 | MS.gene052777:CDS |
| CACCAAGGATGACCCAGCCA+AGG | 0.594197 | 4.4:+86101513 | MS.gene052777:CDS |
| GAGAAAGTCTCAACAACCAT+GGG | 0.598729 | 4.4:-86101754 | None:intergenic |
| TTGACTTCCCAGAGTCGACA+TGG | 0.600770 | 4.4:-86100507 | None:intergenic |
| TCTCCCTCAAAACCAGAGAT+GGG | 0.607478 | 4.4:-86101139 | None:intergenic |
| TCCTGAAGTGGTAGTCGGAG+GGG | 0.608492 | 4.4:-86101262 | None:intergenic |
| AGCGCTCTACAAATCTTGAC+TGG | 0.610562 | 4.4:+86101173 | MS.gene052777:CDS |
| AGAGAAAGTCTCAACAACCA+TGG | 0.614767 | 4.4:-86101755 | None:intergenic |
| GCAAACTGAAGTCAAGTCTG+TGG | 0.619692 | 4.4:+86101384 | MS.gene052777:CDS |
| TCACACCAAGAGTGAAAGCA+AGG | 0.621193 | 4.4:-86100879 | None:intergenic |
| ATCTTGTAGACATCCTGAAG+TGG | 0.621388 | 4.4:-86101274 | None:intergenic |
| CAAGGGCCCAACACTTCTTG+AGG | 0.622743 | 4.4:+86101198 | MS.gene052777:CDS |
| AGCCATTTCCAATCTGGCCA+GGG | 0.623271 | 4.4:-86101576 | None:intergenic |
| ACAGCAAAACGGCCAAGTGG+AGG | 0.624787 | 4.4:-86101784 | None:intergenic |
| TACCTGCTCTTGGAATACTT+GGG | 0.632334 | 4.4:-86101043 | None:intergenic |
| GGAATTGGAACTGTTCCTGT+CGG | 0.634263 | 4.4:+86101301 | MS.gene052777:CDS |
| GGTGTGGAGAAGAAGGAACC+TGG | 0.634359 | 4.4:+86101847 | MS.gene052777:CDS |
| CATCCTGAAGTGGTAGTCGG+AGG | 0.637451 | 4.4:-86101264 | None:intergenic |
| AGACATCCTGAAGTGGTAGT+CGG | 0.638051 | 4.4:-86101267 | None:intergenic |
| CAGCAAACTTGACAGCAATG+TGG | 0.638328 | 4.4:-86101627 | None:intergenic |
| GAGACTGGTACCATGAAACC+TGG | 0.643102 | 4.4:+86101331 | MS.gene052777:CDS |
| AATGATCTGCTGCTGCAACA+AGG | 0.643325 | 4.4:+86100906 | MS.gene052777:CDS |
| TTAAATCCCACATTGTCACC+AGG | 0.648032 | 4.4:-86101439 | None:intergenic |
| TGACAGCAATGTGGGAGGTG+TGG | 0.650950 | 4.4:-86101618 | None:intergenic |
| GCACTTCCTGGTGACAATGT+GGG | 0.653874 | 4.4:+86101433 | MS.gene052777:CDS |
| AATGAAATCCCTGTGTCCGG+GGG | 0.661737 | 4.4:-86100740 | None:intergenic |
| TCTGTCAAGGATCTCAAGCG+TGG | 0.663761 | 4.4:+86101475 | MS.gene052777:CDS |
| CAGAGATATGCGTCAAACTG+TGG | 0.666313 | 4.4:+86101807 | MS.gene052777:CDS |
| CAAGGATGACCCAGCCAAGG+AGG | 0.668893 | 4.4:+86101516 | MS.gene052777:CDS |
| TTCTGGTAAGGAGATCGAGA+AGG | 0.668930 | 4.4:+86101675 | MS.gene052777:CDS |
| CTGACAGCAAAACGGCCAAG+TGG | 0.679918 | 4.4:-86101787 | None:intergenic |
| GTGATTATCATGAATCACCC+TGG | 0.681230 | 4.4:+86101559 | MS.gene052777:CDS |
| TACCATGAAACCTGGAATGG+TGG | 0.682936 | 4.4:+86101339 | MS.gene052777:CDS |
| CTTGTAGATCAAGTGACCAG+TGG | 0.695213 | 4.4:-86100533 | None:intergenic |
| AAACTTGACAGCAATGTGGG+AGG | 0.695398 | 4.4:-86101623 | None:intergenic |
| TCACAATTGACATTGCTCTG+TGG | 0.708352 | 4.4:+86100677 | MS.gene052777:CDS |
| TGGTACCATGAAACCTGGAA+TGG | 0.712415 | 4.4:+86101336 | MS.gene052777:CDS |
| GGTGTGGCAGTCAAGCACAG+GGG | 0.731105 | 4.4:-86101602 | None:intergenic |
| TTATGTTGCCTCAAACACCA+AGG | 0.751394 | 4.4:+86101498 | MS.gene052777:CDS |
| GCATCATGAATCTCTCACTG+AGG | 0.754777 | 4.4:+86101411 | MS.gene052777:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTATAGTTAGTTTATAC+AGG | + | chr4.4:86100979-86100998 | MS.gene052777:intron | 15.0% |
| !! | ATAATCTAATGCAACTAAAT+AGG | - | chr4.4:86100960-86100979 | None:intergenic | 20.0% |
| !! | TAATCTAATGCAACTAAATA+GGG | - | chr4.4:86100959-86100978 | None:intergenic | 20.0% |
| ! | AATCTAATGCAACTAAATAG+GGG | - | chr4.4:86100958-86100977 | None:intergenic | 25.0% |
| ! | TTCATTAAGAACATGATTAC+TGG | + | chr4.4:86100757-86100776 | MS.gene052777:CDS | 25.0% |
| !! | TTTTTGATATCTTGAACAGA+TGG | + | chr4.4:86101009-86101028 | MS.gene052777:intron | 25.0% |
| AGTTTCATCCTATATGAAGA+AGG | + | chr4.4:86101084-86101103 | MS.gene052777:CDS | 30.0% | |
| CACATTAACATTGTTGTCAT+TGG | + | chr4.4:86100484-86100503 | MS.gene052777:CDS | 30.0% | |
| TTAGTTATCTTAACAGTACC+AGG | - | chr4.4:86101868-86101887 | None:intergenic | 30.0% | |
| ! | GAACGAATGGAATTTTATCT+GGG | - | chr4.4:86101120-86101139 | None:intergenic | 30.0% |
| ! | TGTTAAGAATGTTTCTGTCA+AGG | + | chr4.4:86101462-86101481 | MS.gene052777:CDS | 30.0% |
| AATTTCATCATACCTGCTCT+TGG | - | chr4.4:86101056-86101075 | None:intergenic | 35.0% | |
| ACAAATCTTGACTGGTACAA+GGG | + | chr4.4:86101181-86101200 | MS.gene052777:CDS | 35.0% | |
| ATAGCCAACCTTCTTCATAT+AGG | - | chr4.4:86101095-86101114 | None:intergenic | 35.0% | |
| ATCAAATCAATGAGCCTAAG+AGG | + | chr4.4:86101227-86101246 | MS.gene052777:CDS | 35.0% | |
| CAGGTATGATGAAATTGTGA+AGG | + | chr4.4:86101060-86101079 | MS.gene052777:CDS | 35.0% | |
| TACAAATCTTGACTGGTACA+AGG | + | chr4.4:86101180-86101199 | MS.gene052777:CDS | 35.0% | |
| TCATCCTATATGAAGAAGGT+TGG | + | chr4.4:86101088-86101107 | MS.gene052777:CDS | 35.0% | |
| ! | GGAACGAATGGAATTTTATC+TGG | - | chr4.4:86101121-86101140 | None:intergenic | 35.0% |
| !! | AGCTTGTTACTAAGATTGAC+AGG | + | chr4.4:86101650-86101669 | MS.gene052777:CDS | 35.0% |
| !!! | TTTTGAAGCTGGTATTTCCA+AGG | + | chr4.4:86100831-86100850 | MS.gene052777:CDS | 35.0% |
| AGAGAAAGTCTCAACAACCA+TGG | - | chr4.4:86101758-86101777 | None:intergenic | 40.0% | |
| AGCAAACTTGACAGCAATGT+GGG | - | chr4.4:86101629-86101648 | None:intergenic | 40.0% | |
| AGCGTTCATTCAAGTATGCT+TGG | + | chr4.4:86100617-86100636 | MS.gene052777:CDS | 40.0% | |
| ATACCTGCTCTTGGAATACT+TGG | - | chr4.4:86101047-86101066 | None:intergenic | 40.0% | |
| ATCTCCATTCTTCAGGAACT+TGG | - | chr4.4:86101704-86101723 | None:intergenic | 40.0% | |
| ATCTTGTAGACATCCTGAAG+TGG | - | chr4.4:86101277-86101296 | None:intergenic | 40.0% | |
| CTTAATGAAATCCCTGTGTC+CGG | - | chr4.4:86100746-86100765 | None:intergenic | 40.0% | |
| CTTCAGGATGTCTACAAGAT+TGG | + | chr4.4:86101277-86101296 | MS.gene052777:CDS | 40.0% | |
| GAGAAAGTCTCAACAACCAT+GGG | - | chr4.4:86101757-86101776 | None:intergenic | 40.0% | |
| GCATATCTCTGACAGCAAAA+CGG | - | chr4.4:86101798-86101817 | None:intergenic | 40.0% | |
| GCGTTCATTCAAGTATGCTT+GGG | + | chr4.4:86100618-86100637 | MS.gene052777:CDS | 40.0% | |
| GTCTACAAGATTGGAGGAAT+TGG | + | chr4.4:86101286-86101305 | MS.gene052777:CDS | 40.0% | |
| GTGATTATCATGAATCACCC+TGG | + | chr4.4:86101559-86101578 | MS.gene052777:CDS | 40.0% | |
| TACCTGCTCTTGGAATACTT+GGG | - | chr4.4:86101046-86101065 | None:intergenic | 40.0% | |
| TCACAATTGACATTGCTCTG+TGG | + | chr4.4:86100677-86100696 | MS.gene052777:CDS | 40.0% | |
| TCTCCATTCTTCAGGAACTT+GGG | - | chr4.4:86101703-86101722 | None:intergenic | 40.0% | |
| TTAAATCCCACATTGTCACC+AGG | - | chr4.4:86101442-86101461 | None:intergenic | 40.0% | |
| TTAATGAAATCCCTGTGTCC+GGG | - | chr4.4:86100745-86100764 | None:intergenic | 40.0% | |
| TTATGTTGCCTCAAACACCA+AGG | + | chr4.4:86101498-86101517 | MS.gene052777:CDS | 40.0% | |
| TTCTCTGAATATCCTCCACT+TGG | + | chr4.4:86101772-86101791 | MS.gene052777:CDS | 40.0% | |
| AAACCAGAGATGGGAACGAA+TGG | - | chr4.4:86101133-86101152 | None:intergenic | 45.0% | |
| AAACTTGACAGCAATGTGGG+AGG | - | chr4.4:86101626-86101645 | None:intergenic | 45.0% | |
| AATGATCTGCTGCTGCAACA+AGG | + | chr4.4:86100906-86100925 | MS.gene052777:CDS | 45.0% | |
| ACTAAGATTGACAGGCGTTC+TGG | + | chr4.4:86101658-86101677 | MS.gene052777:CDS | 45.0% | |
| ACTTCAGTTTGCAGTCCAGT+TGG | - | chr4.4:86101376-86101395 | None:intergenic | 45.0% | |
| AGACATCCTGAAGTGGTAGT+CGG | - | chr4.4:86101270-86101289 | None:intergenic | 45.0% | |
| AGCGCTCTACAAATCTTGAC+TGG | + | chr4.4:86101173-86101192 | MS.gene052777:CDS | 45.0% | |
| AGCTTCAAAACCACCAGTAG+TGG | - | chr4.4:86100821-86100840 | None:intergenic | 45.0% | |
| ATCTCCCTCAAAACCAGAGA+TGG | - | chr4.4:86101143-86101162 | None:intergenic | 45.0% | |
| ATTCCATTCGTTCCCATCTC+TGG | + | chr4.4:86101127-86101146 | MS.gene052777:CDS | 45.0% | |
| CACTTGATCTACAAGCTTGG+AGG | + | chr4.4:86100541-86100560 | MS.gene052777:CDS | 45.0% | |
| CAGAGATATGCGTCAAACTG+TGG | + | chr4.4:86101807-86101826 | MS.gene052777:CDS | 45.0% | |
| CAGCAAACTTGACAGCAATG+TGG | - | chr4.4:86101630-86101649 | None:intergenic | 45.0% | |
| CAGGATGTCTACAAGATTGG+AGG | + | chr4.4:86101280-86101299 | MS.gene052777:CDS | 45.0% | |
| CTTGGAATACTTGGGTGTAG+TGG | - | chr4.4:86101038-86101057 | None:intergenic | 45.0% | |
| CTTGTAGATCAAGTGACCAG+TGG | - | chr4.4:86100536-86100555 | None:intergenic | 45.0% | |
| GAAGCTGGTATTTCCAAGGA+TGG | + | chr4.4:86100835-86100854 | MS.gene052777:CDS | 45.0% | |
| GAGAACAGCACAATCAGCTT+GGG | - | chr4.4:86100788-86100807 | None:intergenic | 45.0% | |
| GCAAACTGAAGTCAAGTCTG+TGG | + | chr4.4:86101384-86101403 | MS.gene052777:CDS | 45.0% | |
| GCATCATGAATCTCTCACTG+AGG | + | chr4.4:86101411-86101430 | MS.gene052777:CDS | 45.0% | |
| GGAATTGGAACTGTTCCTGT+CGG | + | chr4.4:86101301-86101320 | MS.gene052777:CDS | 45.0% | |
| GGTCACTTGATCTACAAGCT+TGG | + | chr4.4:86100538-86100557 | MS.gene052777:CDS | 45.0% | |
| TAATGAAATCCCTGTGTCCG+GGG | - | chr4.4:86100744-86100763 | None:intergenic | 45.0% | |
| TACCAGCATCTCCATTCTTC+AGG | - | chr4.4:86101711-86101730 | None:intergenic | 45.0% | |
| TACCATGAAACCTGGAATGG+TGG | + | chr4.4:86101339-86101358 | MS.gene052777:CDS | 45.0% | |
| TCACACCAAGAGTGAAAGCA+AGG | - | chr4.4:86100882-86100901 | None:intergenic | 45.0% | |
| TCTCCCTCAAAACCAGAGAT+GGG | - | chr4.4:86101142-86101161 | None:intergenic | 45.0% | |
| TGAGAACAGCACAATCAGCT+TGG | - | chr4.4:86100789-86100808 | None:intergenic | 45.0% | |
| TGGTACCATGAAACCTGGAA+TGG | + | chr4.4:86101336-86101355 | MS.gene052777:CDS | 45.0% | |
| TTCCTGAAGAATGGAGATGC+TGG | + | chr4.4:86101706-86101725 | MS.gene052777:CDS | 45.0% | |
| TTCTGGTAAGGAGATCGAGA+AGG | + | chr4.4:86101675-86101694 | MS.gene052777:CDS | 45.0% | |
| TTGGGTTCTTGACAAGCTCA+AGG | + | chr4.4:86100636-86100655 | MS.gene052777:CDS | 45.0% | |
| ! | AAGCAAGGAGAGCATGTTCA+CGG | - | chr4.4:86100867-86100886 | None:intergenic | 45.0% |
| ! | TATCAAGGGTGTGGAGAAGA+AGG | + | chr4.4:86101840-86101859 | MS.gene052777:CDS | 45.0% |
| ! | TCAAGAGCCTCAAGAAGTGT+TGG | - | chr4.4:86101208-86101227 | None:intergenic | 45.0% |
| ! | TTGCTTTCACTCTTGGTGTG+AGG | + | chr4.4:86100881-86100900 | MS.gene052777:CDS | 45.0% |
| !! | ATCATTGACTCCACTACTGG+TGG | + | chr4.4:86100808-86100827 | MS.gene052777:CDS | 45.0% |
| !! | CAAGAGCCTCAAGAAGTGTT+GGG | - | chr4.4:86101207-86101226 | None:intergenic | 45.0% |
| !! | CTCATCATTGACTCCACTAC+TGG | + | chr4.4:86100805-86100824 | MS.gene052777:CDS | 45.0% |
| !!! | ACTACTGGTGGTTTTGAAGC+TGG | + | chr4.4:86100820-86100839 | MS.gene052777:CDS | 45.0% |
| !!! | GTTCCCATCTCTGGTTTTGA+GGG | + | chr4.4:86101136-86101155 | MS.gene052777:CDS | 45.0% |
| AATGAAATCCCTGTGTCCGG+GGG | - | chr4.4:86100743-86100762 | None:intergenic | 50.0% | |
| AGCCATTTCCAATCTGGCCA+GGG | - | chr4.4:86101579-86101598 | None:intergenic | 50.0% | |
| AGTCTCAACAACCATGGGCT+TGG | - | chr4.4:86101752-86101771 | None:intergenic | 50.0% | |
| ATCCTGAAGTGGTAGTCGGA+GGG | - | chr4.4:86101266-86101285 | None:intergenic | 50.0% | |
| ATGAATCACCCTGGCCAGAT+TGG | + | chr4.4:86101568-86101587 | MS.gene052777:CDS | 50.0% | |
| ATGCGTCAAACTGTGGCTGT+TGG | + | chr4.4:86101814-86101833 | MS.gene052777:CDS | 50.0% | |
| CACCACCATTCCAGGTTTCA+TGG | - | chr4.4:86101344-86101363 | None:intergenic | 50.0% | |
| CACCCAAGTATTCCAAGAGC+AGG | + | chr4.4:86101041-86101060 | MS.gene052777:CDS | 50.0% | |
| GAGACTGGTACCATGAAACC+TGG | + | chr4.4:86101331-86101350 | MS.gene052777:CDS | 50.0% | |
| GAGCCCAAGTTCCTGAAGAA+TGG | + | chr4.4:86101697-86101716 | MS.gene052777:CDS | 50.0% | |
| GATTGACAGGCGTTCTGGTA+AGG | + | chr4.4:86101663-86101682 | MS.gene052777:CDS | 50.0% | |
| GCAAAAGTCACCACCATTCC+AGG | - | chr4.4:86101352-86101371 | None:intergenic | 50.0% | |
| GCACTTCCTGGTGACAATGT+GGG | + | chr4.4:86101433-86101452 | MS.gene052777:CDS | 50.0% | |
| GCTCTCCTTGCTTTCACTCT+TGG | + | chr4.4:86100874-86100893 | MS.gene052777:CDS | 50.0% | |
| GGGCATAGCCATTTCCAATC+TGG | - | chr4.4:86101585-86101604 | None:intergenic | 50.0% | |
| GTGGCTGTTGGGGTTATCAA+GGG | + | chr4.4:86101826-86101845 | MS.gene052777:CDS | 50.0% | |
| TAGCCATTTCCAATCTGGCC+AGG | - | chr4.4:86101580-86101599 | None:intergenic | 50.0% | |
| TCTGGGAAGTCAACTACCAC+TGG | + | chr4.4:86100517-86100536 | MS.gene052777:CDS | 50.0% | |
| TCTGTCAAGGATCTCAAGCG+TGG | + | chr4.4:86101475-86101494 | MS.gene052777:CDS | 50.0% | |
| TGCGTCAAACTGTGGCTGTT+GGG | + | chr4.4:86101815-86101834 | MS.gene052777:CDS | 50.0% | |
| TGTGAAGTTAGCAGCCTCCT+TGG | - | chr4.4:86101533-86101552 | None:intergenic | 50.0% | |
| TGTGGCTGTTGGGGTTATCA+AGG | + | chr4.4:86101825-86101844 | MS.gene052777:CDS | 50.0% | |
| TGTTGGGGTTATCAAGGGTG+TGG | + | chr4.4:86101831-86101850 | MS.gene052777:CDS | 50.0% | |
| TTGACTTCCCAGAGTCGACA+TGG | - | chr4.4:86100510-86100529 | None:intergenic | 50.0% | |
| ! | AGCAAGGAGAGCATGTTCAC+GGG | - | chr4.4:86100866-86100885 | None:intergenic | 50.0% |
| ! | GTGGTGACTTTTGCTCCAAC+TGG | + | chr4.4:86101358-86101377 | MS.gene052777:CDS | 50.0% |
| ! | TCAACAACCATGGGCTTGGT+GGG | - | chr4.4:86101748-86101767 | None:intergenic | 50.0% |
| !! | TCATTGGCCATGTCGACTCT+GGG | + | chr4.4:86100500-86100519 | MS.gene052777:CDS | 50.0% |
| !! | TGGGTCATCCTTGGTGTTTG+AGG | - | chr4.4:86101509-86101528 | None:intergenic | 50.0% |
| !!! | CGTTCCCATCTCTGGTTTTG+AGG | + | chr4.4:86101135-86101154 | MS.gene052777:CDS | 50.0% |
| AAGTTAGCAGCCTCCTTGGC+TGG | - | chr4.4:86101529-86101548 | None:intergenic | 55.0% | |
| ACAGCAAAACGGCCAAGTGG+AGG | - | chr4.4:86101787-86101806 | None:intergenic | 55.0% | |
| AGGTGTGGCAGTCAAGCACA+GGG | - | chr4.4:86101606-86101625 | None:intergenic | 55.0% | |
| AGTTAGCAGCCTCCTTGGCT+GGG | - | chr4.4:86101528-86101547 | None:intergenic | 55.0% | |
| CAAGGGCCCAACACTTCTTG+AGG | + | chr4.4:86101198-86101217 | MS.gene052777:CDS | 55.0% | |
| CACCCTGGCCAGATTGGAAA+TGG | + | chr4.4:86101574-86101593 | MS.gene052777:CDS | 55.0% | |
| CATCCTGAAGTGGTAGTCGG+AGG | - | chr4.4:86101267-86101286 | None:intergenic | 55.0% | |
| CTCAACAACCATGGGCTTGG+TGG | - | chr4.4:86101749-86101768 | None:intergenic | 55.0% | |
| CTCAAGGCTGAGCGTGAAAG+AGG | + | chr4.4:86100652-86100671 | MS.gene052777:CDS | 55.0% | |
| CTGACAGCAAAACGGCCAAG+TGG | - | chr4.4:86101790-86101809 | None:intergenic | 55.0% | |
| GATGATTCCCACCAAGCCCA+TGG | + | chr4.4:86101738-86101757 | MS.gene052777:CDS | 55.0% | |
| GCGTCAAACTGTGGCTGTTG+GGG | + | chr4.4:86101816-86101835 | MS.gene052777:CDS | 55.0% | |
| GGCACTTCCTGGTGACAATG+TGG | + | chr4.4:86101432-86101451 | MS.gene052777:CDS | 55.0% | |
| GGCTTGTCTGATGGCCTCTT+AGG | - | chr4.4:86101244-86101263 | None:intergenic | 55.0% | |
| GGTGTGGAGAAGAAGGAACC+TGG | + | chr4.4:86101847-86101866 | MS.gene052777:CDS | 55.0% | |
| TCCTGAAGTGGTAGTCGGAG+GGG | - | chr4.4:86101265-86101284 | None:intergenic | 55.0% | |
| TCGACAAGCGTGTGATCGAG+AGG | + | chr4.4:86100566-86100585 | MS.gene052777:CDS | 55.0% | |
| TGACAGCAATGTGGGAGGTG+TGG | - | chr4.4:86101621-86101640 | None:intergenic | 55.0% | |
| TGCACTGTCATTGATGCCCC+CGG | + | chr4.4:86100724-86100743 | MS.gene052777:CDS | 55.0% | |
| TTCACGGGTCTGACCATCCT+TGG | - | chr4.4:86100851-86100870 | None:intergenic | 55.0% | |
| ! | TCTCTCACTGAGGCACTTCC+TGG | + | chr4.4:86101421-86101440 | MS.gene052777:CDS | 55.0% |
| !! | GTCATTGGCCATGTCGACTC+TGG | + | chr4.4:86100499-86100518 | MS.gene052777:CDS | 55.0% |
| AGTCGGAGGGGCTTGTCTGA+TGG | - | chr4.4:86101253-86101272 | None:intergenic | 60.0% | |
| CAAGGATGACCCAGCCAAGG+AGG | + | chr4.4:86101516-86101535 | MS.gene052777:CDS | 60.0% | |
| CACCAAGGATGACCCAGCCA+AGG | + | chr4.4:86101513-86101532 | MS.gene052777:CDS | 60.0% | |
| CATTGATGCCCCCGGACACA+GGG | + | chr4.4:86100732-86100751 | MS.gene052777:CDS | 60.0% | |
| CCAGTCTCAACACGTCCGAC+AGG | - | chr4.4:86101319-86101338 | None:intergenic | 60.0% | |
| CCTGTCGGACGTGTTGAGAC+TGG | + | chr4.4:86101316-86101335 | MS.gene052777:CDS | 60.0% | |
| CTCCTTGGCTGGGTCATCCT+TGG | - | chr4.4:86101518-86101537 | None:intergenic | 60.0% | |
| GAGGTGTGGCAGTCAAGCAC+AGG | - | chr4.4:86101607-86101626 | None:intergenic | 60.0% | |
| GGTGTGGCAGTCAAGCACAG+GGG | - | chr4.4:86101605-86101624 | None:intergenic | 60.0% | |
| TCATTGATGCCCCCGGACAC+AGG | + | chr4.4:86100731-86100750 | MS.gene052777:CDS | 60.0% | |
| GCCCCTCCGACTACCACTTC+AGG | + | chr4.4:86101261-86101280 | MS.gene052777:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 86100466 | 86101909 | 86100466 | ID=MS.gene052777 |
| chr4.4 | mRNA | 86100466 | 86101909 | 86100466 | ID=MS.gene052777.t1;Parent=MS.gene052777 |
| chr4.4 | exon | 86100466 | 86100927 | 86100466 | ID=MS.gene052777.t1.exon1;Parent=MS.gene052777.t1 |
| chr4.4 | CDS | 86100466 | 86100927 | 86100466 | ID=cds.MS.gene052777.t1;Parent=MS.gene052777.t1 |
| chr4.4 | exon | 86101028 | 86101909 | 86101028 | ID=MS.gene052777.t1.exon2;Parent=MS.gene052777.t1 |
| chr4.4 | CDS | 86101028 | 86101909 | 86101028 | ID=cds.MS.gene052777.t1;Parent=MS.gene052777.t1 |
| Gene Sequence |
| Protein sequence |
