Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61632.t1 | XP_024637567.1 | 96.9 | 129 | 1 | 1 | 4 | 132 | 13 | 138 | 5.50E-60 | 240.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61632.t1 | Q9XFB0 | 60.0 | 130 | 46 | 3 | 5 | 132 | 24 | 149 | 4.6e-31 | 136.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61632.t1 | G7JER5 | 96.9 | 128 | 1 | 1 | 5 | 132 | 25 | 149 | 2.0e-59 | 238.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene61632.t1 | TF | C2C2-YABBY |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049351 | MS.gene61632 | 0.824851 | 6.45E-54 | -1.69E-46 |
| MS.gene049356 | MS.gene61632 | 0.819879 | 9.21E-53 | -1.69E-46 |
| MS.gene049369 | MS.gene61632 | 0.803704 | 3.07E-49 | -1.69E-46 |
| MS.gene049661 | MS.gene61632 | 0.823531 | 1.32E-53 | -1.69E-46 |
| MS.gene049662 | MS.gene61632 | 0.823846 | 1.11E-53 | -1.69E-46 |
| MS.gene049758 | MS.gene61632 | 0.818346 | 2.06E-52 | -1.69E-46 |
| MS.gene050128 | MS.gene61632 | 0.838016 | 3.69E-57 | -1.69E-46 |
| MS.gene050301 | MS.gene61632 | 0.820326 | 7.28E-53 | -1.69E-46 |
| MS.gene050397 | MS.gene61632 | 0.831813 | 1.35E-55 | -1.69E-46 |
| MS.gene050432 | MS.gene61632 | 0.810705 | 1.01E-50 | -1.69E-46 |
| MS.gene050971 | MS.gene61632 | 0.815913 | 7.24E-52 | -1.69E-46 |
| MS.gene05101 | MS.gene61632 | 0.806635 | 7.48E-50 | -1.69E-46 |
| MS.gene05105 | MS.gene61632 | 0.810911 | 9.11E-51 | -1.69E-46 |
| MS.gene051255 | MS.gene61632 | 0.811492 | 6.81E-51 | -1.69E-46 |
| MS.gene052078 | MS.gene61632 | 0.841552 | 4.43E-58 | -1.69E-46 |
| MS.gene052178 | MS.gene61632 | 0.834623 | 2.69E-56 | -1.69E-46 |
| MS.gene052188 | MS.gene61632 | 0.824653 | 7.18E-54 | -1.69E-46 |
| MS.gene052287 | MS.gene61632 | 0.813065 | 3.09E-51 | -1.69E-46 |
| MS.gene052290 | MS.gene61632 | 0.809061 | 2.28E-50 | -1.69E-46 |
| MS.gene052310 | MS.gene61632 | 0.803006 | 4.28E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene61632 | 0.805324 | 1.41E-49 | -1.69E-46 |
| MS.gene052777 | MS.gene61632 | -0.806176 | 9.34E-50 | -1.69E-46 |
| MS.gene053103 | MS.gene61632 | 0.810875 | 9.27E-51 | -1.69E-46 |
| MS.gene053104 | MS.gene61632 | 0.80919 | 2.14E-50 | -1.69E-46 |
| MS.gene053209 | MS.gene61632 | 0.857785 | 1.29E-62 | -1.69E-46 |
| MS.gene053449 | MS.gene61632 | 0.80376 | 2.99E-49 | -1.69E-46 |
| MS.gene054011 | MS.gene61632 | 0.805242 | 1.47E-49 | -1.69E-46 |
| MS.gene054012 | MS.gene61632 | 0.806042 | 9.97E-50 | -1.69E-46 |
| MS.gene054013 | MS.gene61632 | 0.804215 | 2.40E-49 | -1.69E-46 |
| MS.gene055277 | MS.gene61632 | 0.873075 | 1.95E-67 | -1.69E-46 |
| MS.gene055390 | MS.gene61632 | 0.812417 | 4.28E-51 | -1.69E-46 |
| MS.gene055422 | MS.gene61632 | 0.804699 | 1.91E-49 | -1.69E-46 |
| MS.gene055604 | MS.gene61632 | 0.811808 | 5.82E-51 | -1.69E-46 |
| MS.gene05592 | MS.gene61632 | 0.816139 | 6.45E-52 | -1.69E-46 |
| MS.gene056191 | MS.gene61632 | 0.814116 | 1.81E-51 | -1.69E-46 |
| MS.gene056410 | MS.gene61632 | 0.858572 | 7.51E-63 | -1.69E-46 |
| MS.gene056609 | MS.gene61632 | 0.83893 | 2.15E-57 | -1.69E-46 |
| MS.gene056611 | MS.gene61632 | 0.816585 | 5.13E-52 | -1.69E-46 |
| MS.gene056612 | MS.gene61632 | 0.804042 | 2.61E-49 | -1.69E-46 |
| MS.gene056796 | MS.gene61632 | 0.818436 | 1.96E-52 | -1.69E-46 |
| MS.gene056902 | MS.gene61632 | 0.8023 | 5.99E-49 | -1.69E-46 |
| MS.gene056931 | MS.gene61632 | 0.800204 | 1.61E-48 | -1.69E-46 |
| MS.gene057796 | MS.gene61632 | 0.819283 | 1.26E-52 | -1.69E-46 |
| MS.gene058082 | MS.gene61632 | 0.837914 | 3.92E-57 | -1.69E-46 |
| MS.gene05811 | MS.gene61632 | 0.832225 | 1.07E-55 | -1.69E-46 |
| MS.gene058293 | MS.gene61632 | 0.810723 | 1.00E-50 | -1.69E-46 |
| MS.gene058660 | MS.gene61632 | 0.834955 | 2.22E-56 | -1.69E-46 |
| MS.gene058787 | MS.gene61632 | 0.810895 | 9.18E-51 | -1.69E-46 |
| MS.gene058928 | MS.gene61632 | 0.811201 | 7.88E-51 | -1.69E-46 |
| MS.gene05907 | MS.gene61632 | 0.814202 | 1.74E-51 | -1.69E-46 |
| MS.gene059294 | MS.gene61632 | 0.86353 | 2.33E-64 | -1.69E-46 |
| MS.gene059366 | MS.gene61632 | 0.813518 | 2.46E-51 | -1.69E-46 |
| MS.gene059518 | MS.gene61632 | 0.800262 | 1.56E-48 | -1.69E-46 |
| MS.gene059536 | MS.gene61632 | 0.804785 | 1.83E-49 | -1.69E-46 |
| MS.gene059676 | MS.gene61632 | 0.824224 | 9.06E-54 | -1.69E-46 |
| MS.gene059688 | MS.gene61632 | 0.82019 | 7.82E-53 | -1.69E-46 |
| MS.gene059692 | MS.gene61632 | 0.82907 | 6.32E-55 | -1.69E-46 |
| MS.gene059695 | MS.gene61632 | 0.820655 | 6.12E-53 | -1.69E-46 |
| MS.gene059708 | MS.gene61632 | 0.816763 | 4.68E-52 | -1.69E-46 |
| MS.gene059711 | MS.gene61632 | 0.807806 | 4.22E-50 | -1.69E-46 |
| MS.gene05984 | MS.gene61632 | 0.820562 | 6.42E-53 | -1.69E-46 |
| MS.gene059894 | MS.gene61632 | 0.821539 | 3.83E-53 | -1.69E-46 |
| MS.gene059974 | MS.gene61632 | 0.839125 | 1.91E-57 | -1.69E-46 |
| MS.gene060005 | MS.gene61632 | 0.809592 | 1.75E-50 | -1.69E-46 |
| MS.gene06037 | MS.gene61632 | 0.82387 | 1.10E-53 | -1.69E-46 |
| MS.gene060800 | MS.gene61632 | 0.805702 | 1.17E-49 | -1.69E-46 |
| MS.gene060804 | MS.gene61632 | 0.826763 | 2.27E-54 | -1.69E-46 |
| MS.gene060818 | MS.gene61632 | 0.822143 | 2.77E-53 | -1.69E-46 |
| MS.gene060837 | MS.gene61632 | 0.809558 | 1.78E-50 | -1.69E-46 |
| MS.gene061041 | MS.gene61632 | 0.809868 | 1.53E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene61632 | 0.816317 | 5.89E-52 | -1.69E-46 |
| MS.gene061051 | MS.gene61632 | -0.832387 | 9.73E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61632.t1 | MTR_4g050300 | 96.875 | 128 | 1 | 1 | 5 | 132 | 25 | 149 | 8.69e-84 | 246 |
| MS.gene61632.t1 | MTR_2g087740 | 71.642 | 134 | 30 | 4 | 5 | 132 | 28 | 159 | 6.86e-51 | 163 |
| MS.gene61632.t1 | MTR_4g114730 | 56.589 | 129 | 50 | 4 | 5 | 132 | 30 | 153 | 1.04e-32 | 116 |
| MS.gene61632.t1 | MTR_5g034030 | 48.507 | 134 | 59 | 3 | 5 | 132 | 28 | 157 | 9.19e-31 | 112 |
| MS.gene61632.t1 | MTR_4g025900 | 47.917 | 144 | 51 | 4 | 5 | 132 | 39 | 174 | 3.32e-28 | 105 |
| MS.gene61632.t1 | MTR_4g101660 | 45.890 | 146 | 51 | 4 | 5 | 132 | 34 | 169 | 3.60e-27 | 102 |
| MS.gene61632.t1 | MTR_5g046230 | 43.846 | 130 | 60 | 2 | 5 | 132 | 33 | 151 | 2.95e-22 | 90.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61632.t1 | AT1G08465 | 60.000 | 130 | 46 | 3 | 5 | 132 | 24 | 149 | 2.58e-43 | 143 |
| MS.gene61632.t1 | AT2G26580 | 58.594 | 128 | 43 | 3 | 5 | 132 | 25 | 142 | 1.63e-33 | 117 |
| MS.gene61632.t1 | AT2G26580 | 58.594 | 128 | 43 | 3 | 5 | 132 | 25 | 142 | 1.63e-33 | 117 |
| MS.gene61632.t1 | AT2G45190 | 52.414 | 145 | 47 | 4 | 5 | 132 | 39 | 178 | 7.64e-33 | 117 |
| MS.gene61632.t1 | AT4G00180 | 47.826 | 161 | 50 | 5 | 1 | 132 | 4 | 159 | 9.13e-28 | 104 |
| MS.gene61632.t1 | AT4G00180 | 47.771 | 157 | 48 | 5 | 5 | 132 | 39 | 190 | 6.40e-24 | 95.1 |
| MS.gene61632.t1 | AT1G23420 | 39.855 | 138 | 73 | 2 | 5 | 132 | 35 | 172 | 1.70e-23 | 93.6 |
| MS.gene61632.t1 | AT1G23420 | 39.855 | 138 | 73 | 2 | 5 | 132 | 66 | 203 | 7.13e-23 | 92.8 |
| MS.gene61632.t1 | AT2G45190 | 72.727 | 66 | 16 | 2 | 69 | 132 | 49 | 114 | 1.39e-22 | 89.7 |
| MS.gene61632.t1 | AT1G69180 | 38.636 | 132 | 63 | 2 | 5 | 132 | 35 | 152 | 1.62e-19 | 82.0 |
Find 44 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGCGCTCGATTCAGTTT+TGG | 0.249533 | 4.1:-47568336 | None:intergenic |
| CGGCGGTTTGTGTTGGGTTT+TGG | 0.255996 | 4.1:-47568256 | None:intergenic |
| TTGGTGAGCTATTGCGGTCT+GGG | 0.259203 | 4.1:-47568237 | None:intergenic |
| TCCACATCTAACTGTTACTA+TGG | 0.283760 | 4.1:-47555013 | None:intergenic |
| GTGGCTGATATCTGGATTAC+TGG | 0.287474 | 4.1:-47558532 | None:intergenic |
| GCCTCCCTGTGGCTGATATC+TGG | 0.289400 | 4.1:-47558540 | None:intergenic |
| TTTGGTGAGCTATTGCGGTC+TGG | 0.304440 | 4.1:-47568238 | None:intergenic |
| TTTGGTTAGCTTCGATTTGC+AGG | 0.317070 | 4.1:-47568306 | None:intergenic |
| GAAGCAAGCAGCAAGGAAAT+TGG | 0.345303 | 4.1:+47557027 | MS.gene61632:CDS |
| GGAGGAAATTCAAAGGATTA+AGG | 0.360502 | 4.1:+47558509 | MS.gene61632:CDS |
| GCTTGAATGCTTGTGTTTCA+CGG | 0.395536 | 4.1:-47568162 | None:intergenic |
| ATATAACTTACTTAATGAAC+CGG | 0.422652 | 4.1:-47558151 | None:intergenic |
| GAAGTTTGAAATGGATTGGC+AGG | 0.437811 | 4.1:+47554963 | None:intergenic |
| GGGAGTTGAGGAGGAGGAAA+TGG | 0.440779 | 4.1:-47555084 | None:intergenic |
| CGACGAATGGGAGGAATCCT+AGG | 0.445790 | 4.1:-47557111 | None:intergenic |
| ATGGTTAACAAACTGCTGTA+TGG | 0.448489 | 4.1:-47554994 | None:intergenic |
| GTGTTCCTTCTGCTTACAAC+CGG | 0.449198 | 4.1:+47558132 | MS.gene61632:CDS |
| TCCTCCTCCTCAACTCCCTC+AGG | 0.474865 | 4.1:+47555089 | MS.gene61632:CDS |
| CAACAAAATAGCATCATTTG+AGG | 0.493091 | 4.1:+47557068 | MS.gene61632:CDS |
| TGGTGAGCTATTGCGGTCTG+GGG | 0.502413 | 4.1:-47568236 | None:intergenic |
| AGAGTGTGACTCACGACGAA+TGG | 0.516345 | 4.1:-47557124 | None:intergenic |
| TAGTAGAGCACGAACATCCT+AGG | 0.516780 | 4.1:+47557094 | MS.gene61632:CDS |
| GGTGAGCTATTGCGGTCTGG+GGG | 0.527148 | 4.1:-47568235 | None:intergenic |
| GAGTGTGACTCACGACGAAT+GGG | 0.531160 | 4.1:-47557123 | None:intergenic |
| ACCTGAGGGAGTTGAGGAGG+AGG | 0.539948 | 4.1:-47555090 | None:intergenic |
| TGAATTATGTGCTGTTTCTG+TGG | 0.544740 | 4.1:-47557000 | None:intergenic |
| GGAGGAAATGGCTGAAGTAA+TGG | 0.547935 | 4.1:-47555072 | None:intergenic |
| ACACAAACCGCCGCAAAATG+AGG | 0.551926 | 4.1:+47568266 | MS.gene61632:CDS |
| AAACATACCTGAGGGAGTTG+AGG | 0.577695 | 4.1:-47555096 | None:intergenic |
| ATGAACCGGTTGTAAGCAGA+AGG | 0.582569 | 4.1:-47558137 | None:intergenic |
| GTAATCCAGATATCAGCCAC+AGG | 0.588300 | 4.1:+47558535 | MS.gene61632:CDS |
| TAATTTGCTATCTGTTAACA+TGG | 0.605095 | 4.1:+47555044 | MS.gene61632:CDS |
| TCTGTAGGGAGGAAATTCAA+AGG | 0.621966 | 4.1:+47558502 | MS.gene61632:intron |
| CTGAGCTGAAAGCCTCCCTG+TGG | 0.623676 | 4.1:-47558551 | None:intergenic |
| GTGAGCTATTGCGGTCTGGG+GGG | 0.625495 | 4.1:-47568234 | None:intergenic |
| CAGGGAGGCTTTCAGCTCAG+CGG | 0.634037 | 4.1:+47558554 | MS.gene61632:CDS |
| CATACCTGAGGGAGTTGAGG+AGG | 0.635233 | 4.1:-47555093 | None:intergenic |
| ACCATAGTAACAGTTAGATG+TGG | 0.643235 | 4.1:+47555012 | MS.gene61632:CDS |
| TGTGACTCACGACGAATGGG+AGG | 0.645995 | 4.1:-47557120 | None:intergenic |
| CTACACCATAACCAGACACA+AGG | 0.653649 | 4.1:+47568139 | MS.gene61632:intron |
| TCATGAAGAAGCAAGCAGCA+AGG | 0.659976 | 4.1:+47557020 | MS.gene61632:CDS |
| TTTGCTATCTGTTAACATGG+TGG | 0.679420 | 4.1:+47555047 | MS.gene61632:CDS |
| TCCAGATATCAGCCACAGGG+AGG | 0.683704 | 4.1:+47558539 | MS.gene61632:CDS |
| TAATCCAGATATCAGCCACA+GGG | 0.696359 | 4.1:+47558536 | MS.gene61632:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 47554973 | 47568345 | 47554973 | ID=MS.gene61632 |
| chr4.1 | mRNA | 47554973 | 47568345 | 47554973 | ID=MS.gene61632.t1;Parent=MS.gene61632 |
| chr4.1 | exon | 47554973 | 47555110 | 47554973 | ID=MS.gene61632.t1.exon1;Parent=MS.gene61632.t1 |
| chr4.1 | CDS | 47554973 | 47555110 | 47554973 | ID=cds.MS.gene61632.t1;Parent=MS.gene61632.t1 |
| chr4.1 | exon | 47557000 | 47557132 | 47557000 | ID=MS.gene61632.t1.exon2;Parent=MS.gene61632.t1 |
| chr4.1 | CDS | 47557000 | 47557132 | 47557000 | ID=cds.MS.gene61632.t1;Parent=MS.gene61632.t1 |
| chr4.1 | exon | 47558114 | 47558162 | 47558114 | ID=MS.gene61632.t1.exon3;Parent=MS.gene61632.t1 |
| chr4.1 | CDS | 47558114 | 47558162 | 47558114 | ID=cds.MS.gene61632.t1;Parent=MS.gene61632.t1 |
| chr4.1 | exon | 47558509 | 47558584 | 47558509 | ID=MS.gene61632.t1.exon4;Parent=MS.gene61632.t1 |
| chr4.1 | CDS | 47558509 | 47558584 | 47558509 | ID=cds.MS.gene61632.t1;Parent=MS.gene61632.t1 |
| chr4.1 | exon | 47568154 | 47568345 | 47568154 | ID=MS.gene61632.t1.exon5;Parent=MS.gene61632.t1 |
| chr4.1 | CDS | 47568154 | 47568345 | 47568154 | ID=cds.MS.gene61632.t1;Parent=MS.gene61632.t1 |
| Gene Sequence |
| Protein sequence |
