AlfalfaGEDB | MS.gene87404

M. sativa cultivar XinJiangDaYe / MS.gene87404

KEGG: K13789
GO: GO:0006629 GO:0006720 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009987 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0071704 GO:1901576
PFAM: PF00348.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87404.t1	ADG01841.1	99.7	356	1	0	1	356	15	370	2.90E-192	681

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87404.t1	Q94ID7	74.9	351	83	2	11	356	20	370	1.2e-138	494.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87404.t1	D6Q1T4	99.7	356	1	0	1	356	15	370	2.1e-192	681.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049137	MS.gene87404	0.802161	6.40E-49	-1.69E-46
MS.gene049217	MS.gene87404	0.802919	4.46E-49	-1.69E-46
MS.gene049222	MS.gene87404	0.821602	3.70E-53	-1.69E-46
MS.gene049356	MS.gene87404	0.88764	1.19E-72	-1.69E-46
MS.gene049369	MS.gene87404	0.907513	4.67E-81	-1.69E-46
MS.gene049396	MS.gene87404	0.859208	4.85E-63	-1.69E-46
MS.gene049609	MS.gene87404	0.815259	1.01E-51	-1.69E-46
MS.gene049805	MS.gene87404	0.831593	1.53E-55	-1.69E-46
MS.gene049828	MS.gene87404	0.814531	1.47E-51	-1.69E-46
MS.gene049907	MS.gene87404	0.824129	9.54E-54	-1.69E-46
MS.gene050122	MS.gene87404	0.82722	1.76E-54	-1.69E-46
MS.gene050380	MS.gene87404	-0.804595	2.00E-49	-1.69E-46
MS.gene050499	MS.gene87404	0.849077	4.08E-60	-1.69E-46
MS.gene050502	MS.gene87404	0.806833	6.79E-50	-1.69E-46
MS.gene050520	MS.gene87404	0.842976	1.86E-58	-1.69E-46
MS.gene050670	MS.gene87404	0.81821	2.21E-52	-1.69E-46
MS.gene050686	MS.gene87404	0.849499	3.11E-60	-1.69E-46
MS.gene050747	MS.gene87404	0.8239	1.08E-53	-1.69E-46
MS.gene050772	MS.gene87404	0.831622	1.50E-55	-1.69E-46
MS.gene050950	MS.gene87404	0.816963	4.22E-52	-1.69E-46
MS.gene05105	MS.gene87404	0.830207	3.34E-55	-1.69E-46
MS.gene051255	MS.gene87404	0.800006	1.76E-48	-1.69E-46
MS.gene051360	MS.gene87404	0.801234	9.91E-49	-1.69E-46
MS.gene051387	MS.gene87404	0.840986	6.25E-58	-1.69E-46
MS.gene051509	MS.gene87404	0.825702	4.06E-54	-1.69E-46
MS.gene051666	MS.gene87404	0.840816	6.92E-58	-1.69E-46
MS.gene051692	MS.gene87404	0.825202	5.33E-54	-1.69E-46
MS.gene051858	MS.gene87404	0.80985	1.54E-50	-1.69E-46
MS.gene051874	MS.gene87404	0.801721	7.88E-49	-1.69E-46
MS.gene052164	MS.gene87404	0.802802	4.72E-49	-1.69E-46
MS.gene052188	MS.gene87404	0.807296	5.42E-50	-1.69E-46
MS.gene052414	MS.gene87404	0.827408	1.59E-54	-1.69E-46
MS.gene052421	MS.gene87404	0.842863	1.99E-58	-1.69E-46
MS.gene052485	MS.gene87404	0.820767	5.76E-53	-1.69E-46
MS.gene052615	MS.gene87404	0.809327	2.00E-50	-1.69E-46
MS.gene052632	MS.gene87404	0.838864	2.23E-57	-1.69E-46
MS.gene052777	MS.gene87404	-0.818535	1.86E-52	-1.69E-46
MS.gene053018	MS.gene87404	0.822592	2.18E-53	-1.69E-46
MS.gene053103	MS.gene87404	0.810289	1.24E-50	-1.69E-46
MS.gene053104	MS.gene87404	0.821394	4.13E-53	-1.69E-46
MS.gene053207	MS.gene87404	0.813244	2.82E-51	-1.69E-46
MS.gene053213	MS.gene87404	0.817618	3.00E-52	-1.69E-46
MS.gene053286	MS.gene87404	0.813823	2.11E-51	-1.69E-46
MS.gene053436	MS.gene87404	0.807927	3.98E-50	-1.69E-46
MS.gene053449	MS.gene87404	0.849504	3.10E-60	-1.69E-46
MS.gene053505	MS.gene87404	0.836081	1.15E-56	-1.69E-46
MS.gene053574	MS.gene87404	0.844868	5.79E-59	-1.69E-46
MS.gene053626	MS.gene87404	0.839201	1.83E-57	-1.69E-46
MS.gene053632	MS.gene87404	0.817991	2.47E-52	-1.69E-46
MS.gene053758	MS.gene87404	0.812601	3.90E-51	-1.69E-46
MS.gene053813	MS.gene87404	0.85651	3.07E-62	-1.69E-46
MS.gene053912	MS.gene87404	0.846744	1.79E-59	-1.69E-46
MS.gene053916	MS.gene87404	0.868183	7.89E-66	-1.69E-46
MS.gene054003	MS.gene87404	0.802027	6.82E-49	-1.69E-46
MS.gene054123	MS.gene87404	0.833026	6.75E-56	-1.69E-46
MS.gene054486	MS.gene87404	0.839455	1.57E-57	-1.69E-46
MS.gene054595	MS.gene87404	0.803512	3.36E-49	-1.69E-46
MS.gene054642	MS.gene87404	0.800683	1.28E-48	-1.69E-46
MS.gene05494	MS.gene87404	0.838969	2.10E-57	-1.69E-46
MS.gene054955	MS.gene87404	0.813743	2.19E-51	-1.69E-46
MS.gene05505	MS.gene87404	0.889723	1.88E-73	-1.69E-46
MS.gene055224	MS.gene87404	0.817715	2.86E-52	-1.69E-46
MS.gene055486	MS.gene87404	0.818404	2.00E-52	-1.69E-46
MS.gene055577	MS.gene87404	0.805011	1.64E-49	-1.69E-46
MS.gene055722	MS.gene87404	0.820935	5.27E-53	-1.69E-46
MS.gene055909	MS.gene87404	0.868309	7.19E-66	-1.69E-46
MS.gene055911	MS.gene87404	0.867818	1.03E-65	-1.69E-46
MS.gene05592	MS.gene87404	0.849954	2.32E-60	-1.69E-46
MS.gene055941	MS.gene87404	0.820922	5.31E-53	-1.69E-46
MS.gene056407	MS.gene87404	0.802356	5.83E-49	-1.69E-46
MS.gene056422	MS.gene87404	0.873595	1.30E-67	-1.69E-46
MS.gene056448	MS.gene87404	0.83474	2.52E-56	-1.69E-46
MS.gene056487	MS.gene87404	0.861383	1.07E-63	-1.69E-46
MS.gene056511	MS.gene87404	0.869368	3.26E-66	-1.69E-46
MS.gene056609	MS.gene87404	0.80786	4.11E-50	-1.69E-46
MS.gene05664	MS.gene87404	0.847463	1.14E-59	-1.69E-46
MS.gene056657	MS.gene87404	0.813709	2.23E-51	-1.69E-46
MS.gene05666	MS.gene87404	0.868308	7.19E-66	-1.69E-46
MS.gene056942	MS.gene87404	0.817552	3.11E-52	-1.69E-46
MS.gene056947	MS.gene87404	0.812209	4.76E-51	-1.69E-46
MS.gene057076	MS.gene87404	0.825783	3.88E-54	-1.69E-46
MS.gene057086	MS.gene87404	0.83623	1.06E-56	-1.69E-46
MS.gene057146	MS.gene87404	0.804768	1.84E-49	-1.69E-46
MS.gene05735	MS.gene87404	0.847803	9.17E-60	-1.69E-46
MS.gene057416	MS.gene87404	0.811784	5.89E-51	-1.69E-46
MS.gene057537	MS.gene87404	0.835097	2.05E-56	-1.69E-46
MS.gene057734	MS.gene87404	0.853362	2.51E-61	-1.69E-46
MS.gene057749	MS.gene87404	0.801273	9.73E-49	-1.69E-46
MS.gene057823	MS.gene87404	0.821801	3.33E-53	-1.69E-46
MS.gene05787	MS.gene87404	0.80929	2.04E-50	-1.69E-46
MS.gene057926	MS.gene87404	0.875594	2.72E-68	-1.69E-46
MS.gene057927	MS.gene87404	0.836011	1.20E-56	-1.69E-46
MS.gene058006	MS.gene87404	0.802627	5.13E-49	-1.69E-46
MS.gene058007	MS.gene87404	0.800207	1.61E-48	-1.69E-46
MS.gene058019	MS.gene87404	0.801341	9.43E-49	-1.69E-46
MS.gene058075	MS.gene87404	0.886438	3.42E-72	-1.69E-46
MS.gene058090	MS.gene87404	0.806824	6.82E-50	-1.69E-46
MS.gene058120	MS.gene87404	0.828555	8.42E-55	-1.69E-46
MS.gene058130	MS.gene87404	0.826441	2.71E-54	-1.69E-46
MS.gene058323	MS.gene87404	0.817889	2.61E-52	-1.69E-46
MS.gene058376	MS.gene87404	0.819751	9.85E-53	-1.69E-46
MS.gene058459	MS.gene87404	0.846461	2.14E-59	-1.69E-46
MS.gene058660	MS.gene87404	0.810513	1.11E-50	-1.69E-46
MS.gene058788	MS.gene87404	0.80986	1.54E-50	-1.69E-46
MS.gene058790	MS.gene87404	0.839191	1.84E-57	-1.69E-46
MS.gene058928	MS.gene87404	0.844653	6.62E-59	-1.69E-46
MS.gene058948	MS.gene87404	0.854465	1.21E-61	-1.69E-46
MS.gene05913	MS.gene87404	0.824967	6.06E-54	-1.69E-46
MS.gene059305	MS.gene87404	0.825935	3.57E-54	-1.69E-46
MS.gene05934	MS.gene87404	0.828569	8.36E-55	-1.69E-46
MS.gene059366	MS.gene87404	0.836029	1.19E-56	-1.69E-46
MS.gene059367	MS.gene87404	0.871325	7.45E-67	-1.69E-46
MS.gene059368	MS.gene87404	0.882854	7.34E-71	-1.69E-46
MS.gene059536	MS.gene87404	0.814274	1.67E-51	-1.69E-46
MS.gene05959	MS.gene87404	0.804767	1.84E-49	-1.69E-46
MS.gene059674	MS.gene87404	0.867773	1.07E-65	-1.69E-46
MS.gene059680	MS.gene87404	0.806572	7.71E-50	-1.69E-46
MS.gene059688	MS.gene87404	0.818697	1.71E-52	-1.69E-46
MS.gene059711	MS.gene87404	0.879893	8.59E-70	-1.69E-46
MS.gene059718	MS.gene87404	0.807727	4.39E-50	-1.69E-46
MS.gene059807	MS.gene87404	0.863951	1.72E-64	-1.69E-46
MS.gene05986	MS.gene87404	0.810982	8.79E-51	-1.69E-46
MS.gene059870	MS.gene87404	0.811392	7.16E-51	-1.69E-46
MS.gene060193	MS.gene87404	0.805987	1.02E-49	-1.69E-46
MS.gene060372	MS.gene87404	0.810531	1.10E-50	-1.69E-46
MS.gene060403	MS.gene87404	0.803833	2.89E-49	-1.69E-46
MS.gene060482	MS.gene87404	0.852793	3.66E-61	-1.69E-46
MS.gene060804	MS.gene87404	0.879764	9.55E-70	-1.69E-46
MS.gene060818	MS.gene87404	0.833355	5.59E-56	-1.69E-46
MS.gene060835	MS.gene87404	0.838941	2.13E-57	-1.69E-46
MS.gene060837	MS.gene87404	0.883486	4.31E-71	-1.69E-46
MS.gene06097	MS.gene87404	0.84294	1.90E-58	-1.69E-46
MS.gene061041	MS.gene87404	0.901028	4.05E-78	-1.69E-46
MS.gene061051	MS.gene87404	-0.83613	1.12E-56	-1.69E-46
MS.gene061134	MS.gene87404	0.823875	1.09E-53	-1.69E-46
MS.gene061200	MS.gene87404	0.830073	3.61E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87404.t1	MTR_8g078070	97.493	359	5	2	1	356	15	372	0.0	704
MS.gene87404.t1	MTR_5g019460	73.684	323	85	0	34	356	49	371	2.20e-176	494
MS.gene87404.t1	MTR_2g072470	37.751	249	135	7	76	317	113	348	1.78e-36	137
MS.gene87404.t1	MTR_5g092910	32.039	206	131	2	76	277	54	254	2.28e-24	101
MS.gene87404.t1	MTR_4g095055	31.984	247	137	7	91	317	103	338	6.75e-21	93.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87404.t1	AT4G36810	74.571	350	82	5	11	356	25	371	0.0	510
MS.gene87404.t1	AT2G18620	72.647	340	87	3	22	356	9	347	1.22e-174	489
MS.gene87404.t1	AT2G18640	69.257	296	87	2	63	356	79	372	9.79e-150	427
MS.gene87404.t1	AT2G23800	68.562	299	90	2	60	356	80	376	2.85e-148	423
MS.gene87404.t1	AT3G20160	66.221	299	94	4	63	356	48	344	2.26e-142	407
MS.gene87404.t1	AT3G14550	67.257	339	100	5	28	356	23	360	3.90e-142	407
MS.gene87404.t1	AT3G14550	67.257	339	100	5	28	356	23	360	3.90e-142	407
MS.gene87404.t1	AT3G14550	67.257	339	100	5	28	356	23	360	3.90e-142	407
MS.gene87404.t1	AT3G14530	65.782	339	105	5	28	356	23	360	4.31e-140	402
MS.gene87404.t1	AT3G29430	70.492	305	87	2	55	356	53	357	2.39e-138	397
MS.gene87404.t1	AT3G14510	72.563	277	73	2	83	356	8	284	3.66e-138	394
MS.gene87404.t1	AT3G32040	68.852	305	92	2	55	356	56	360	2.04e-135	390
MS.gene87404.t1	AT3G20160	60.473	296	88	4	63	356	48	316	1.63e-123	358
MS.gene87404.t1	AT1G49530	59.344	305	111	4	65	356	32	336	3.01e-123	358
MS.gene87404.t1	AT4G38460	47.083	240	118	4	63	299	45	278	5.05e-72	227
MS.gene87404.t1	AT1G78510	36.404	228	128	5	102	323	84	300	1.76e-33	127
MS.gene87404.t1	AT1G78510	36.404	228	128	5	102	323	123	339	4.62e-33	127
MS.gene87404.t1	AT1G17050	30.816	331	207	9	3	323	32	350	2.14e-32	125
MS.gene87404.t1	AT2G34630	31.443	194	122	3	134	327	171	353	9.23e-19	87.4
MS.gene87404.t1	AT2G34630	31.443	194	122	3	134	327	70	252	1.17e-18	85.9

Find 74 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATATATCGGTTTATTGTTTC+AGG	0.214767	8.3:-26098024	MS.gene87404:CDS
TGGCTAATTACATTGCTTAT+AGG	0.225968	8.3:-26097800	MS.gene87404:CDS
GATGCACAAGAACAACTTTC+TGG	0.227841	8.3:-26097858	MS.gene87404:CDS
TTAGAAGGTGCTGTTGTTCT+TGG	0.252595	8.3:-26098104	MS.gene87404:CDS
TGAAACTGAAATGGGTATTT+TGG	0.254823	8.3:+26098772	None:intergenic
CCGAAGACTTTGTGGTTTGT+AGG	0.273097	8.3:+26098365	None:intergenic
GTTGTTCTTGGTGCAATATT+AGG	0.288632	8.3:-26098092	MS.gene87404:CDS
CGAAAATTCGCGAGATATAT+CGG	0.296068	8.3:-26098038	MS.gene87404:CDS
ATCTGCTCCTTTGATTGCTT+TGG	0.315020	8.3:-26097820	MS.gene87404:CDS
AAAACTGCGGCTTTGTTAGA+AGG	0.342543	8.3:-26098119	MS.gene87404:CDS
CGTCTTCTCCGAAGACTTTG+TGG	0.343711	8.3:+26098357	None:intergenic
AAAATAGAAAGATGGGATTC+TGG	0.344376	8.3:+26098796	None:intergenic
TAATGAAACGGCGTCGTCTA+AGG	0.368203	8.3:+26098604	None:intergenic
GGCTCGGAAGGACTCGTTGC+TGG	0.376904	8.3:-26098215	MS.gene87404:CDS
GAAAGATTTGGTAGCTGATA+AGG	0.381248	8.3:-26097943	MS.gene87404:CDS
TAGGATTTGAAATTGAAGAT+TGG	0.396930	8.3:+26098659	None:intergenic
AGGATTTGAAATTGAAGATT+GGG	0.398523	8.3:+26098660	None:intergenic
CCACGACGACCTTCCTTGTA+TGG	0.400090	8.3:-26098414	MS.gene87404:CDS
TAAAACTGCTGGGAAAGATT+TGG	0.402177	8.3:-26097955	MS.gene87404:CDS
TTTAATGGGTGAAACTGAAA+TGG	0.414231	8.3:+26098763	None:intergenic
CAGGCGGCCGGCATCGCCAT+CGG	0.414541	8.3:+26098470	None:intergenic
GGCCGGCATCGCCATCGGTT+CGG	0.427454	8.3:+26098475	None:intergenic
GAAAGATGGGATTCTGGATC+TGG	0.430542	8.3:+26098802	None:intergenic
ACGAAATCTTCACAGGAGTT+AGG	0.431856	8.3:-26097978	MS.gene87404:CDS
ATTTCAACGGCGCAGGCGGC+CGG	0.436594	8.3:+26098458	None:intergenic
ATGCACAAGAACAACTTTCT+GGG	0.437672	8.3:-26097857	MS.gene87404:CDS
GCGAGAATTGTCCGTGCAAT+CGG	0.446559	8.3:-26098257	MS.gene87404:CDS
TAACGGTTCGCGTAATGAAA+CGG	0.452982	8.3:+26098592	None:intergenic
AACCGAACCGATGGCGATGC+CGG	0.457738	8.3:-26098477	MS.gene87404:CDS
CCTACAAACCACAAAGTCTT+CGG	0.467203	8.3:-26098365	MS.gene87404:CDS
GGTGAATTAGCAAAATCAAT+TGG	0.470164	8.3:-26098236	MS.gene87404:CDS
CAGGAGTTAGGTAAAACTGC+TGG	0.471173	8.3:-26097966	MS.gene87404:CDS
CGCCGGAGAAACACCGGCGG+TGG	0.473224	8.3:+26098277	None:intergenic
CTCCACCGCCGGTGTTTCTC+CGG	0.475973	8.3:-26098279	MS.gene87404:CDS
CAGGCGGCTAAACAGAGAAC+TGG	0.480639	8.3:+26098515	None:intergenic
TCGCCGGCGGAAAACGTGTC+CGG	0.481378	8.3:-26098538	MS.gene87404:CDS
AAAAGCGAGAAGAGCGTCAC+CGG	0.482767	8.3:+26098322	None:intergenic
GTATGGATAACGACGATCTC+CGG	0.512700	8.3:-26098397	MS.gene87404:CDS
TTGCTAATTCACCGATTGCA+CGG	0.521598	8.3:+26098246	None:intergenic
GTGGATCATTTCAACGGCGC+AGG	0.524802	8.3:+26098451	None:intergenic
TCAGAAGGTTTATCCGATGT+TGG	0.530197	8.3:-26098173	MS.gene87404:CDS
GCAAAATCAATTGGCTCGGA+AGG	0.538303	8.3:-26098227	MS.gene87404:CDS
GGTTTGTAGGTTTACCTCGC+CGG	0.539958	8.3:+26098378	None:intergenic
GGTTCCGCCGACTAGTTCGC+AGG	0.541501	8.3:+26098496	None:intergenic
TTAATGGGTGAAACTGAAAT+GGG	0.544577	8.3:+26098764	None:intergenic
GTTGTTGATATCAACTCAGA+AGG	0.546305	8.3:-26098188	MS.gene87404:CDS
GATCATTTCAACGGCGCAGG+CGG	0.547898	8.3:+26098454	None:intergenic
TGATGTGACGAAATCTTCAC+AGG	0.548692	8.3:-26097985	MS.gene87404:CDS
CATATCGCTGTCTCCACCGC+CGG	0.579244	8.3:-26098290	MS.gene87404:CDS
AATTCTCGCCGGAGAAACAC+CGG	0.583236	8.3:+26098271	None:intergenic
ATTAGCAAAATCAATTGGCT+CGG	0.586837	8.3:-26098231	MS.gene87404:CDS
GAAGACGTTGCTGTTCTCGC+CGG	0.587256	8.3:-26098341	MS.gene87404:CDS
AAAGTTGTTCTTGTGCATCA+CGG	0.594174	8.3:+26097862	None:intergenic
AGGAGTTAGGTAAAACTGCT+GGG	0.596113	8.3:-26097965	MS.gene87404:CDS
CATCGTGTGGATCATTTCAA+CGG	0.597412	8.3:+26098445	None:intergenic
AGATCGTCGTTATCCATACA+AGG	0.598288	8.3:+26098401	None:intergenic
ATGCGTTACTCTCTCCTCGC+CGG	0.606596	8.3:-26098554	MS.gene87404:CDS
TAATTAGCCAAAGCAATCAA+AGG	0.617462	8.3:+26097813	None:intergenic
GTTCTTGGTGCAATATTAGG+TGG	0.624578	8.3:-26098089	MS.gene87404:CDS
CTTGGTGCAATATTAGGTGG+TGG	0.625172	8.3:-26098086	MS.gene87404:CDS
CGTCGTTATCCATACAAGGA+AGG	0.629409	8.3:+26098405	None:intergenic
CGGCTAAACAGAGAACTGGC+CGG	0.632818	8.3:+26098519	None:intergenic
CATTCATCTGCATAAAACTG+CGG	0.633100	8.3:-26098132	MS.gene87404:CDS
AGTCGGCGGAACCGAACCGA+TGG	0.634309	8.3:-26098486	MS.gene87404:CDS
CGTTACTCTCTCCTCGCCGG+CGG	0.638430	8.3:-26098551	MS.gene87404:CDS
TTAGCCGCCTGCGAACTAGT+CGG	0.649753	8.3:-26098503	MS.gene87404:CDS
CCATACAAGGAAGGTCGTCG+TGG	0.653424	8.3:+26098414	None:intergenic
AAGCTGTTCGAGTCCAACAT+CGG	0.661724	8.3:+26098160	None:intergenic
CGTGGATTAGAGACATCGTG+TGG	0.678617	8.3:+26098432	None:intergenic
TCCGCCGACTAGTTCGCAGG+CGG	0.679352	8.3:+26098499	None:intergenic
GCCGCCTGCGAACTAGTCGG+CGG	0.683331	8.3:-26098500	MS.gene87404:CDS
ATTGCACGGACAATTCTCGC+CGG	0.689391	8.3:+26098260	None:intergenic
GATAACGACGATCTCCGGCG+AGG	0.695961	8.3:-26098392	MS.gene87404:CDS
TCTCGCCGGAGAAACACCGG+CGG	0.762110	8.3:+26098274	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTGGTAAAATAGAAAGA+TGG	+	chr8.3:26097845-26097864	None:intergenic	20.0%
!!!	TTTTGGTAAAATAGAAAGAT+GGG	+	chr8.3:26097844-26097863	None:intergenic	20.0%
!	GAAACAGAAAAAGAAGAATT+GGG	+	chr8.3:26097905-26097924	None:intergenic	25.0%
!	TGAAACAGAAAAAGAAGAAT+TGG	+	chr8.3:26097906-26097925	None:intergenic	25.0%
!!	AGGATTTGAAATTGAAGATT+GGG	+	chr8.3:26097973-26097992	None:intergenic	25.0%
!!	TAGGATTTGAAATTGAAGAT+TGG	+	chr8.3:26097974-26097993	None:intergenic	25.0%
!!	TTTTCAATTCCTAAAAGCTT+AGG	+	chr8.3:26098721-26098740	None:intergenic	25.0%
!!!	ATATATCGGTTTATTGTTTC+AGG	-	chr8.3:26098606-26098625	MS.gene87404:CDS	25.0%
	AAAATAGAAAGATGGGATTC+TGG	+	chr8.3:26097837-26097856	None:intergenic	30.0%
	ACATGATAGAAAAAGCAACT+AGG	-	chr8.3:26097993-26098012	MS.gene87404:CDS	30.0%
	ATTAGCAAAATCAATTGGCT+CGG	-	chr8.3:26098399-26098418	MS.gene87404:CDS	30.0%
	CATGATAGAAAAAGCAACTA+GGG	-	chr8.3:26097994-26098013	MS.gene87404:CDS	30.0%
	GGTGAATTAGCAAAATCAAT+TGG	-	chr8.3:26098394-26098413	MS.gene87404:CDS	30.0%
	TAATTAGCCAAAGCAATCAA+AGG	+	chr8.3:26098820-26098839	None:intergenic	30.0%
	TGGCTAATTACATTGCTTAT+AGG	-	chr8.3:26098830-26098849	MS.gene87404:CDS	30.0%
!	GTTACTTATCCTAAGCTTTT+AGG	-	chr8.3:26098709-26098728	MS.gene87404:CDS	30.0%
!	TGAAACTGAAATGGGTATTT+TGG	+	chr8.3:26097861-26097880	None:intergenic	30.0%
!	TTAATGGGTGAAACTGAAAT+GGG	+	chr8.3:26097869-26097888	None:intergenic	30.0%
!	TTTAATGGGTGAAACTGAAA+TGG	+	chr8.3:26097870-26097889	None:intergenic	30.0%
!	TTTTGTTCTTCTGCTTCTAT+TGG	+	chr8.3:26097950-26097969	None:intergenic	30.0%
!!!	AGTTGCTTTTTCTATCATGT+AGG	+	chr8.3:26097993-26098012	None:intergenic	30.0%
!!!	GGTTTTGATGATGGTTTAAT+GGG	+	chr8.3:26097884-26097903	None:intergenic	30.0%
	AAAGTTGTTCTTGTGCATCA+CGG	+	chr8.3:26098771-26098790	None:intergenic	35.0%
	ATGCACAAGAACAACTTTCT+GGG	-	chr8.3:26098773-26098792	MS.gene87404:CDS	35.0%
	CATTCATCTGCATAAAACTG+CGG	-	chr8.3:26098498-26098517	MS.gene87404:CDS	35.0%
	CGAAAATTCGCGAGATATAT+CGG	-	chr8.3:26098592-26098611	MS.gene87404:CDS	35.0%
	GTTGTTGATATCAACTCAGA+AGG	-	chr8.3:26098442-26098461	MS.gene87404:CDS	35.0%
	TAAAACTGCTGGGAAAGATT+TGG	-	chr8.3:26098675-26098694	MS.gene87404:CDS	35.0%
!	ATCGCTTCGTGAACTTTTAA+CGG	+	chr8.3:26098058-26098077	None:intergenic	35.0%
!	GAAAGATTTGGTAGCTGATA+AGG	-	chr8.3:26098687-26098706	MS.gene87404:CDS	35.0%
!	GTTGTTCTTGGTGCAATATT+AGG	-	chr8.3:26098538-26098557	MS.gene87404:CDS	35.0%
!!	GAAGAATTGGGTTTTGATGA+TGG	+	chr8.3:26097893-26097912	None:intergenic	35.0%
!!!	GGGTTTTGATGATGGTTTAA+TGG	+	chr8.3:26097885-26097904	None:intergenic	35.0%
	ACGAAATCTTCACAGGAGTT+AGG	-	chr8.3:26098652-26098671	MS.gene87404:CDS	40.0%
	AGATCGTCGTTATCCATACA+AGG	+	chr8.3:26098232-26098251	None:intergenic	40.0%
	AGGAGTTAGGTAAAACTGCT+GGG	-	chr8.3:26098665-26098684	MS.gene87404:CDS	40.0%
	ATCTGCTCCTTTGATTGCTT+TGG	-	chr8.3:26098810-26098829	MS.gene87404:CDS	40.0%
	CATCGTGTGGATCATTTCAA+CGG	+	chr8.3:26098188-26098207	None:intergenic	40.0%
	CCTACAAACCACAAAGTCTT+CGG	-	chr8.3:26098265-26098284	MS.gene87404:CDS	40.0%
	GATGCACAAGAACAACTTTC+TGG	-	chr8.3:26098772-26098791	MS.gene87404:CDS	40.0%
	TAACGGTTCGCGTAATGAAA+CGG	+	chr8.3:26098041-26098060	None:intergenic	40.0%
	TCAGAAGGTTTATCCGATGT+TGG	-	chr8.3:26098457-26098476	MS.gene87404:CDS	40.0%
	TGATGTGACGAAATCTTCAC+AGG	-	chr8.3:26098645-26098664	MS.gene87404:CDS	40.0%
!	GTTCTTGGTGCAATATTAGG+TGG	-	chr8.3:26098541-26098560	MS.gene87404:CDS	40.0%
!	TTGCTAATTCACCGATTGCA+CGG	+	chr8.3:26098387-26098406	None:intergenic	40.0%
!!	AAAACTGCGGCTTTGTTAGA+AGG	-	chr8.3:26098511-26098530	MS.gene87404:CDS	40.0%
!!	TTAGAAGGTGCTGTTGTTCT+TGG	-	chr8.3:26098526-26098545	MS.gene87404:CDS	40.0%
	AAGCTGTTCGAGTCCAACAT+CGG	+	chr8.3:26098473-26098492	None:intergenic	45.0%
	CAGGAGTTAGGTAAAACTGC+TGG	-	chr8.3:26098664-26098683	MS.gene87404:CDS	45.0%
	CGTCGTTATCCATACAAGGA+AGG	+	chr8.3:26098228-26098247	None:intergenic	45.0%
	GAAAGATGGGATTCTGGATC+TGG	+	chr8.3:26097831-26097850	None:intergenic	45.0%
	GTATGGATAACGACGATCTC+CGG	-	chr8.3:26098233-26098252	MS.gene87404:CDS	45.0%
	TAATGAAACGGCGTCGTCTA+AGG	+	chr8.3:26098029-26098048	None:intergenic	45.0%
!	CCGAAGACTTTGTGGTTTGT+AGG	+	chr8.3:26098268-26098287	None:intergenic	45.0%
!	CTTGGTGCAATATTAGGTGG+TGG	-	chr8.3:26098544-26098563	MS.gene87404:CDS	45.0%
!!	GCAAAATCAATTGGCTCGGA+AGG	-	chr8.3:26098403-26098422	MS.gene87404:CDS	45.0%
	AATTCTCGCCGGAGAAACAC+CGG	+	chr8.3:26098362-26098381	None:intergenic	50.0%
	ATTGCACGGACAATTCTCGC+CGG	+	chr8.3:26098373-26098392	None:intergenic	50.0%
	CGTCTTCTCCGAAGACTTTG+TGG	+	chr8.3:26098276-26098295	None:intergenic	50.0%
	GCGAGAATTGTCCGTGCAAT+CGG	-	chr8.3:26098373-26098392	MS.gene87404:CDS	50.0%
	GGTTTGTAGGTTTACCTCGC+CGG	+	chr8.3:26098255-26098274	None:intergenic	50.0%
!!	AAAAGCGAGAAGAGCGTCAC+CGG	+	chr8.3:26098311-26098330	None:intergenic	50.0%
!!	CGTGGATTAGAGACATCGTG+TGG	+	chr8.3:26098201-26098220	None:intergenic	50.0%
	ATGCGTTACTCTCTCCTCGC+CGG	-	chr8.3:26098076-26098095	MS.gene87404:CDS	55.0%
	CAGGCGGCTAAACAGAGAAC+TGG	+	chr8.3:26098118-26098137	None:intergenic	55.0%
	CCACGACGACCTTCCTTGTA+TGG	-	chr8.3:26098216-26098235	MS.gene87404:CDS	55.0%
	CCATACAAGGAAGGTCGTCG+TGG	+	chr8.3:26098219-26098238	None:intergenic	55.0%
	CGGCTAAACAGAGAACTGGC+CGG	+	chr8.3:26098114-26098133	None:intergenic	55.0%
	GATCATTTCAACGGCGCAGG+CGG	+	chr8.3:26098179-26098198	None:intergenic	55.0%
	GTGGATCATTTCAACGGCGC+AGG	+	chr8.3:26098182-26098201	None:intergenic	55.0%
	TTAGCCGCCTGCGAACTAGT+CGG	-	chr8.3:26098127-26098146	MS.gene87404:CDS	55.0%
!	GAAGACGTTGCTGTTCTCGC+CGG	-	chr8.3:26098289-26098308	MS.gene87404:CDS	55.0%
	AACCGAACCGATGGCGATGC+CGG	-	chr8.3:26098153-26098172	MS.gene87404:CDS	60.0%
	CATATCGCTGTCTCCACCGC+CGG	-	chr8.3:26098340-26098359	MS.gene87404:CDS	60.0%
	GATAACGACGATCTCCGGCG+AGG	-	chr8.3:26098238-26098257	MS.gene87404:CDS	60.0%
	AGTCGGCGGAACCGAACCGA+TGG	-	chr8.3:26098144-26098163	MS.gene87404:CDS	65.0%
	ATTTCAACGGCGCAGGCGGC+CGG	+	chr8.3:26098175-26098194	None:intergenic	65.0%
	CGTTACTCTCTCCTCGCCGG+CGG	-	chr8.3:26098079-26098098	MS.gene87404:CDS	65.0%
	CTCCACCGCCGGTGTTTCTC+CGG	-	chr8.3:26098351-26098370	MS.gene87404:CDS	65.0%
	GGCTCGGAAGGACTCGTTGC+TGG	-	chr8.3:26098415-26098434	MS.gene87404:CDS	65.0%
	TCGCCGGCGGAAAACGTGTC+CGG	-	chr8.3:26098092-26098111	MS.gene87404:CDS	65.0%
	TCTCGCCGGAGAAACACCGG+CGG	+	chr8.3:26098359-26098378	None:intergenic	65.0%
!	GGTTCCGCCGACTAGTTCGC+AGG	+	chr8.3:26098137-26098156	None:intergenic	65.0%
!	TCCGCCGACTAGTTCGCAGG+CGG	+	chr8.3:26098134-26098153	None:intergenic	65.0%
!	TGGCCGGACACGTTTTCCGC+CGG	+	chr8.3:26098098-26098117	None:intergenic	65.0%
	GCCGCCTGCGAACTAGTCGG+CGG	-	chr8.3:26098130-26098149	MS.gene87404:CDS	70.0%
!	GGACACGTTTTCCGCCGGCG+AGG	+	chr8.3:26098093-26098112	None:intergenic	70.0%
!	GGCCGGCATCGCCATCGGTT+CGG	+	chr8.3:26098158-26098177	None:intergenic	70.0%
	CAGGCGGCCGGCATCGCCAT+CGG	+	chr8.3:26098163-26098182	None:intergenic	75.0%
	CGCCGGAGAAACACCGGCGG+TGG	+	chr8.3:26098356-26098375	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	26097791	26098861	26097791	ID=MS.gene87404
chr8.3	mRNA	26097791	26098861	26097791	ID=MS.gene87404.t1;Parent=MS.gene87404
chr8.3	exon	26097791	26098861	26097791	ID=MS.gene87404.t1.exon1;Parent=MS.gene87404.t1
chr8.3	CDS	26097791	26098861	26097791	ID=cds.MS.gene87404.t1;Parent=MS.gene87404.t1

Gene Sequence

>MS.gene87404

ATGTTCAATCAAGTTACTACTACTGCTACAACTAGATCCAGATCCAGAATCCCATCTTTCTATTTTACCAAAATACCCATTTCAGTTTCACCCATTAAACCATCATCAAAACCCAATTCTTCTTTTTCTGTTTCATCATTACTCACAAAACAAGAACCAATAGAAGCAGAAGAACAAAACCCAATCTTCAATTTCAAATCCTACATGATAGAAAAAGCAACTAGGGTTAACAAAGCCTTAGACGACGCCGTTTCATTACGCGAACCGTTAAAAGTTCACGAAGCGATGCGTTACTCTCTCCTCGCCGGCGGAAAACGTGTCCGGCCAGTTCTCTGTTTAGCCGCCTGCGAACTAGTCGGCGGAACCGAACCGATGGCGATGCCGGCCGCCTGCGCCGTTGAAATGATCCACACGATGTCTCTAATCCACGACGACCTTCCTTGTATGGATAACGACGATCTCCGGCGAGGTAAACCTACAAACCACAAAGTCTTCGGAGAAGACGTTGCTGTTCTCGCCGGTGACGCTCTTCTCGCTTTTGCTTTCGAACATATCGCTGTCTCCACCGCCGGTGTTTCTCCGGCGAGAATTGTCCGTGCAATCGGTGAATTAGCAAAATCAATTGGCTCGGAAGGACTCGTTGCTGGACAAGTTGTTGATATCAACTCAGAAGGTTTATCCGATGTTGGACTCGAACAGCTTGAATTCATTCATCTGCATAAAACTGCGGCTTTGTTAGAAGGTGCTGTTGTTCTTGGTGCAATATTAGGTGGTGGAAGTGATGAAGATGTTGAAAAACTGCGAAAATTCGCGAGATATATCGGTTTATTGTTTCAGGTTGTTGATGATATTCTTGATGTGACGAAATCTTCACAGGAGTTAGGTAAAACTGCTGGGAAAGATTTGGTAGCTGATAAGGTTACTTATCCTAAGCTTTTAGGAATTGAAAAATCGAAAGAGTTTGCTGAGAAATTGAACCGTGATGCACAAGAACAACTTTCTGGGTTTGATTTGAATAAATCTGCTCCTTTGATTGCTTTGGCTAATTACATTGCTTATAGGCAAAACTAA

Protein sequence

>MS.gene87404.t1

MFNQVTTTATTRSRSRIPSFYFTKIPISVSPIKPSSKPNSSFSVSSLLTKQEPIEAEEQNPIFNFKSYMIEKATRVNKALDDAVSLREPLKVHEAMRYSLLAGGKRVRPVLCLAACELVGGTEPMAMPAACAVEMIHTMSLIHDDLPCMDNDDLRRGKPTNHKVFGEDVAVLAGDALLAFAFEHIAVSTAGVSPARIVRAIGELAKSIGSEGLVAGQVVDINSEGLSDVGLEQLEFIHLHKTAALLEGAVVLGAILGGGSDEDVEKLRKFARYIGLLFQVVDDILDVTKSSQELGKTAGKDLVADKVTYPKLLGIEKSKEFAEKLNRDAQEQLSGFDLNKSAPLIALANYIAYRQN