Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56057.t1 | XP_003588559.1 | 95.7 | 325 | 14 | 0 | 1 | 325 | 1 | 325 | 4.90E-178 | 633.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56057.t1 | Q08298 | 43.7 | 389 | 142 | 8 | 5 | 322 | 7 | 389 | 1.4e-77 | 291.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56057.t1 | B7FI52 | 95.7 | 325 | 14 | 0 | 1 | 325 | 1 | 325 | 3.5e-178 | 633.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene56057 | 0.818926 | 1.52E-52 | -1.69E-46 |
| MS.gene049153 | MS.gene56057 | 0.818136 | 2.29E-52 | -1.69E-46 |
| MS.gene049346 | MS.gene56057 | 0.818603 | 1.80E-52 | -1.69E-46 |
| MS.gene049426 | MS.gene56057 | 0.805799 | 1.12E-49 | -1.69E-46 |
| MS.gene049910 | MS.gene56057 | 0.815874 | 7.39E-52 | -1.69E-46 |
| MS.gene049915 | MS.gene56057 | 0.833678 | 4.64E-56 | -1.69E-46 |
| MS.gene050139 | MS.gene56057 | 0.802992 | 4.31E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene56057 | 0.837006 | 6.70E-57 | -1.69E-46 |
| MS.gene050227 | MS.gene56057 | 0.825656 | 4.16E-54 | -1.69E-46 |
| MS.gene050228 | MS.gene56057 | 0.826429 | 2.73E-54 | -1.69E-46 |
| MS.gene050398 | MS.gene56057 | 0.815034 | 1.14E-51 | -1.69E-46 |
| MS.gene050438 | MS.gene56057 | 0.814311 | 1.64E-51 | -1.69E-46 |
| MS.gene050442 | MS.gene56057 | 0.818846 | 1.58E-52 | -1.69E-46 |
| MS.gene050558 | MS.gene56057 | 0.807261 | 5.51E-50 | -1.69E-46 |
| MS.gene050560 | MS.gene56057 | 0.807519 | 4.86E-50 | -1.69E-46 |
| MS.gene050586 | MS.gene56057 | 0.839761 | 1.31E-57 | -1.69E-46 |
| MS.gene050724 | MS.gene56057 | 0.808803 | 2.59E-50 | -1.69E-46 |
| MS.gene050820 | MS.gene56057 | 0.815856 | 7.46E-52 | -1.69E-46 |
| MS.gene051265 | MS.gene56057 | 0.831685 | 1.45E-55 | -1.69E-46 |
| MS.gene051789 | MS.gene56057 | 0.808706 | 2.72E-50 | -1.69E-46 |
| MS.gene051860 | MS.gene56057 | 0.828431 | 9.02E-55 | -1.69E-46 |
| MS.gene052187 | MS.gene56057 | 0.815152 | 1.07E-51 | -1.69E-46 |
| MS.gene052255 | MS.gene56057 | 0.853247 | 2.71E-61 | -1.69E-46 |
| MS.gene05288 | MS.gene56057 | 0.814549 | 1.46E-51 | -1.69E-46 |
| MS.gene05289 | MS.gene56057 | 0.829945 | 3.87E-55 | -1.69E-46 |
| MS.gene052902 | MS.gene56057 | 0.817336 | 3.48E-52 | -1.69E-46 |
| MS.gene053074 | MS.gene56057 | 0.882819 | 7.56E-71 | -1.69E-46 |
| MS.gene053184 | MS.gene56057 | 0.8512 | 1.04E-60 | -1.69E-46 |
| MS.gene053494 | MS.gene56057 | 0.804109 | 2.53E-49 | -1.69E-46 |
| MS.gene053584 | MS.gene56057 | 0.830197 | 3.36E-55 | -1.69E-46 |
| MS.gene053836 | MS.gene56057 | 0.824272 | 8.83E-54 | -1.69E-46 |
| MS.gene054183 | MS.gene56057 | 0.802961 | 4.38E-49 | -1.69E-46 |
| MS.gene054184 | MS.gene56057 | 0.864225 | 1.42E-64 | -1.69E-46 |
| MS.gene054185 | MS.gene56057 | 0.867808 | 1.04E-65 | -1.69E-46 |
| MS.gene05434 | MS.gene56057 | 0.803396 | 3.56E-49 | -1.69E-46 |
| MS.gene054596 | MS.gene56057 | 0.827654 | 1.39E-54 | -1.69E-46 |
| MS.gene054713 | MS.gene56057 | 0.80489 | 1.74E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene56057 | 0.80129 | 9.65E-49 | -1.69E-46 |
| MS.gene055085 | MS.gene56057 | 0.814186 | 1.75E-51 | -1.69E-46 |
| MS.gene055356 | MS.gene56057 | 0.808373 | 3.20E-50 | -1.69E-46 |
| MS.gene055358 | MS.gene56057 | 0.808443 | 3.09E-50 | -1.69E-46 |
| MS.gene055359 | MS.gene56057 | 0.811761 | 5.95E-51 | -1.69E-46 |
| MS.gene055860 | MS.gene56057 | 0.81701 | 4.12E-52 | -1.69E-46 |
| MS.gene057394 | MS.gene56057 | 0.800365 | 1.49E-48 | -1.69E-46 |
| MS.gene057469 | MS.gene56057 | 0.824965 | 6.07E-54 | -1.69E-46 |
| MS.gene057750 | MS.gene56057 | 0.872915 | 2.20E-67 | -1.69E-46 |
| MS.gene057754 | MS.gene56057 | 0.86732 | 1.49E-65 | -1.69E-46 |
| MS.gene057869 | MS.gene56057 | 0.803204 | 3.90E-49 | -1.69E-46 |
| MS.gene058002 | MS.gene56057 | 0.812246 | 4.67E-51 | -1.69E-46 |
| MS.gene058168 | MS.gene56057 | 0.890917 | 6.38E-74 | -1.69E-46 |
| MS.gene059006 | MS.gene56057 | 0.854579 | 1.12E-61 | -1.69E-46 |
| MS.gene059124 | MS.gene56057 | 0.964133 | 6.05E-123 | -1.69E-46 |
| MS.gene059128 | MS.gene56057 | 0.847031 | 1.50E-59 | -1.69E-46 |
| MS.gene059421 | MS.gene56057 | 0.851367 | 9.29E-61 | -1.69E-46 |
| MS.gene05984 | MS.gene56057 | 0.802276 | 6.06E-49 | -1.69E-46 |
| MS.gene060683 | MS.gene56057 | 0.803016 | 4.26E-49 | -1.69E-46 |
| MS.gene060685 | MS.gene56057 | 0.800853 | 1.19E-48 | -1.69E-46 |
| MS.gene060998 | MS.gene56057 | 0.828899 | 6.96E-55 | -1.69E-46 |
| MS.gene061085 | MS.gene56057 | 0.813001 | 3.19E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56057.t1 | MTR_0312s0030 | 95.385 | 325 | 15 | 0 | 1 | 325 | 1 | 325 | 0.0 | 646 |
| MS.gene56057.t1 | MTR_1g007520 | 95.385 | 325 | 15 | 0 | 1 | 325 | 1 | 325 | 0.0 | 646 |
| MS.gene56057.t1 | MTR_1g008590 | 95.692 | 325 | 14 | 0 | 1 | 325 | 1 | 325 | 0.0 | 619 |
| MS.gene56057.t1 | MTR_1g008510 | 75.076 | 329 | 75 | 5 | 1 | 325 | 1 | 326 | 4.01e-178 | 496 |
| MS.gene56057.t1 | MTR_1g008560 | 73.353 | 334 | 76 | 6 | 1 | 325 | 1 | 330 | 6.38e-175 | 488 |
| MS.gene56057.t1 | MTR_1g008500 | 63.753 | 389 | 73 | 7 | 1 | 325 | 1 | 385 | 1.23e-164 | 464 |
| MS.gene56057.t1 | MTR_1g008470 | 62.883 | 326 | 94 | 5 | 1 | 325 | 1 | 300 | 7.79e-142 | 403 |
| MS.gene56057.t1 | MTR_3g109490 | 59.327 | 327 | 122 | 6 | 1 | 323 | 1 | 320 | 9.33e-140 | 398 |
| MS.gene56057.t1 | MTR_1g008450 | 61.677 | 334 | 98 | 7 | 1 | 325 | 1 | 313 | 1.44e-138 | 395 |
| MS.gene56057.t1 | MTR_1g008460 | 59.091 | 330 | 97 | 7 | 1 | 325 | 1 | 297 | 6.95e-126 | 362 |
| MS.gene56057.t1 | MTR_1g008530 | 76.712 | 219 | 48 | 2 | 110 | 325 | 6 | 224 | 1.20e-122 | 351 |
| MS.gene56057.t1 | MTR_1g008580 | 78.824 | 170 | 35 | 1 | 155 | 323 | 1 | 170 | 5.38e-97 | 284 |
| MS.gene56057.t1 | MTR_3g107830 | 42.411 | 224 | 122 | 4 | 108 | 325 | 34 | 256 | 1.02e-55 | 182 |
| MS.gene56057.t1 | MTR_4g069680 | 42.857 | 224 | 121 | 5 | 108 | 325 | 65 | 287 | 2.02e-54 | 180 |
| MS.gene56057.t1 | MTR_3g116410 | 40.179 | 224 | 127 | 4 | 108 | 325 | 54 | 276 | 3.12e-53 | 176 |
| MS.gene56057.t1 | MTR_3g116380 | 43.049 | 223 | 117 | 7 | 108 | 323 | 82 | 301 | 4.01e-53 | 177 |
| MS.gene56057.t1 | MTR_1g008420 | 48.438 | 192 | 76 | 5 | 9 | 178 | 9 | 199 | 9.52e-45 | 152 |
| MS.gene56057.t1 | MTR_3g116270 | 38.605 | 215 | 124 | 4 | 113 | 322 | 292 | 503 | 4.36e-44 | 158 |
| MS.gene56057.t1 | MTR_3g116320 | 39.070 | 215 | 123 | 4 | 113 | 322 | 474 | 685 | 1.33e-43 | 159 |
| MS.gene56057.t1 | MTR_4g069040 | 36.017 | 236 | 140 | 7 | 95 | 323 | 277 | 508 | 3.00e-41 | 150 |
| MS.gene56057.t1 | MTR_4g069570 | 36.697 | 218 | 128 | 6 | 113 | 323 | 120 | 334 | 3.61e-41 | 146 |
| MS.gene56057.t1 | MTR_4g069550 | 36.239 | 218 | 129 | 6 | 113 | 323 | 247 | 461 | 1.70e-39 | 145 |
| MS.gene56057.t1 | MTR_8g064500 | 36.574 | 216 | 127 | 5 | 113 | 322 | 159 | 370 | 2.38e-38 | 140 |
| MS.gene56057.t1 | MTR_2g081610 | 29.538 | 325 | 153 | 13 | 4 | 323 | 5 | 258 | 9.44e-37 | 133 |
| MS.gene56057.t1 | MTR_4g069520 | 34.404 | 218 | 133 | 6 | 113 | 323 | 507 | 721 | 3.88e-35 | 135 |
| MS.gene56057.t1 | MTR_4g069520 | 34.862 | 218 | 132 | 6 | 113 | 323 | 493 | 707 | 5.29e-35 | 135 |
| MS.gene56057.t1 | MTR_5g034320 | 33.333 | 213 | 138 | 4 | 113 | 322 | 415 | 626 | 6.67e-35 | 134 |
| MS.gene56057.t1 | MTR_2g081590 | 29.283 | 321 | 159 | 11 | 4 | 323 | 5 | 258 | 1.28e-34 | 127 |
| MS.gene56057.t1 | MTR_8g064560 | 34.579 | 214 | 134 | 4 | 113 | 322 | 412 | 623 | 1.82e-34 | 133 |
| MS.gene56057.t1 | MTR_8g046000 | 38.073 | 218 | 103 | 9 | 112 | 323 | 30 | 221 | 1.20e-33 | 124 |
| MS.gene56057.t1 | MTR_2g081770 | 28.239 | 301 | 149 | 10 | 26 | 323 | 33 | 269 | 1.90e-32 | 122 |
| MS.gene56057.t1 | MTR_3g078090 | 33.953 | 215 | 134 | 4 | 113 | 322 | 413 | 624 | 1.91e-32 | 127 |
| MS.gene56057.t1 | MTR_8g445170 | 39.610 | 154 | 87 | 4 | 170 | 323 | 235 | 382 | 1.97e-32 | 124 |
| MS.gene56057.t1 | MTR_8g045890 | 44.056 | 143 | 74 | 4 | 181 | 323 | 294 | 430 | 5.12e-32 | 124 |
| MS.gene56057.t1 | MTR_8g064530 | 33.333 | 213 | 138 | 4 | 113 | 322 | 429 | 640 | 8.27e-32 | 125 |
| MS.gene56057.t1 | MTR_8g045890 | 44.056 | 143 | 74 | 4 | 181 | 323 | 335 | 471 | 8.57e-32 | 124 |
| MS.gene56057.t1 | MTR_8g045890 | 44.056 | 143 | 74 | 4 | 181 | 323 | 372 | 508 | 9.23e-32 | 124 |
| MS.gene56057.t1 | MTR_6g086530 | 33.493 | 209 | 128 | 7 | 125 | 325 | 1 | 206 | 1.02e-31 | 118 |
| MS.gene56057.t1 | MTR_8g445140 | 41.259 | 143 | 78 | 4 | 181 | 323 | 165 | 301 | 1.46e-31 | 120 |
| MS.gene56057.t1 | MTR_1g045860 | 27.607 | 326 | 165 | 10 | 1 | 323 | 1 | 258 | 1.57e-30 | 116 |
| MS.gene56057.t1 | MTR_4g133840 | 32.447 | 188 | 109 | 4 | 138 | 322 | 14 | 186 | 2.17e-30 | 114 |
| MS.gene56057.t1 | MTR_8g045880 | 42.069 | 145 | 78 | 4 | 179 | 323 | 175 | 313 | 4.88e-29 | 114 |
| MS.gene56057.t1 | MTR_8g045880 | 42.069 | 145 | 78 | 4 | 179 | 323 | 210 | 348 | 7.72e-29 | 114 |
| MS.gene56057.t1 | MTR_8g045880 | 42.069 | 145 | 78 | 4 | 179 | 323 | 212 | 350 | 1.01e-28 | 114 |
| MS.gene56057.t1 | MTR_8g044290 | 29.102 | 323 | 173 | 15 | 14 | 289 | 19 | 332 | 3.67e-24 | 102 |
| MS.gene56057.t1 | MTR_5g033340 | 26.852 | 216 | 152 | 4 | 111 | 322 | 164 | 377 | 3.91e-22 | 95.9 |
| MS.gene56057.t1 | MTR_5g033340 | 28.378 | 148 | 101 | 3 | 162 | 306 | 8 | 153 | 1.12e-12 | 68.6 |
| MS.gene56057.t1 | MTR_4g068420 | 35.556 | 135 | 68 | 5 | 192 | 323 | 1 | 119 | 2.25e-21 | 88.6 |
| MS.gene56057.t1 | MTR_5g033350 | 27.660 | 188 | 130 | 4 | 139 | 322 | 13 | 198 | 3.10e-19 | 84.7 |
| MS.gene56057.t1 | MTR_5g033330 | 25.926 | 216 | 154 | 4 | 111 | 322 | 403 | 616 | 3.73e-19 | 88.6 |
| MS.gene56057.t1 | MTR_5g033330 | 26.500 | 200 | 141 | 4 | 111 | 306 | 195 | 392 | 8.64e-17 | 81.6 |
| MS.gene56057.t1 | MTR_5g033330 | 26.744 | 172 | 120 | 4 | 139 | 306 | 15 | 184 | 7.11e-13 | 69.7 |
| MS.gene56057.t1 | MTR_8g075930 | 43.038 | 79 | 41 | 2 | 238 | 313 | 10 | 87 | 3.73e-17 | 75.9 |
| MS.gene56057.t1 | MTR_5g033360 | 25.116 | 215 | 139 | 5 | 111 | 322 | 25 | 220 | 3.29e-16 | 77.0 |
| MS.gene56057.t1 | MTR_5g033430 | 32.941 | 85 | 55 | 2 | 240 | 322 | 24 | 108 | 9.73e-12 | 61.6 |
| MS.gene56057.t1 | MTR_5g033440 | 32.558 | 86 | 56 | 2 | 240 | 323 | 24 | 109 | 2.17e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56057.t1 | AT5G25610 | 43.846 | 390 | 142 | 11 | 5 | 323 | 7 | 390 | 3.43e-99 | 298 |
| MS.gene56057.t1 | AT1G49320 | 37.662 | 231 | 132 | 6 | 106 | 325 | 51 | 280 | 3.01e-47 | 160 |
| MS.gene56057.t1 | AT1G60390 | 36.111 | 216 | 128 | 5 | 113 | 322 | 409 | 620 | 7.38e-35 | 134 |
| MS.gene56057.t1 | AT1G70370 | 35.514 | 214 | 132 | 4 | 113 | 322 | 411 | 622 | 1.16e-33 | 130 |
| MS.gene56057.t1 | AT1G70370 | 35.514 | 214 | 132 | 4 | 113 | 322 | 411 | 622 | 1.16e-33 | 130 |
| MS.gene56057.t1 | AT1G23760 | 34.112 | 214 | 135 | 4 | 113 | 322 | 407 | 618 | 5.10e-31 | 123 |
Find 67 sgRNAs with CRISPR-Local
Find 109 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTGAAATGCCAAATGTTT+TGG | 0.214978 | 1.3:+11343494 | None:intergenic |
| AAATGTAGCACTCTTCTTTC+TGG | 0.227762 | 1.3:-11344050 | MS.gene56057:CDS |
| TCTTACCTCCTCAACTTTAC+TGG | 0.262120 | 1.3:-11344881 | MS.gene56057:CDS |
| ATTTATCTGAAGCTGGATAT+TGG | 0.302370 | 1.3:-11344237 | MS.gene56057:intron |
| GCAGCATAATTATAAATAAA+TGG | 0.303775 | 1.3:+11344100 | None:intergenic |
| TGTAACATCATTGGAGTCTA+TGG | 0.322922 | 1.3:-11343795 | MS.gene56057:CDS |
| TGCCTGAGGATCATGTTGTT+TGG | 0.331746 | 1.3:-11343430 | MS.gene56057:CDS |
| AGCAGATTAGTGATGGCTTT+TGG | 0.347892 | 1.3:+11344831 | None:intergenic |
| TTCCACCTTGTTTGATGAAA+AGG | 0.348656 | 1.3:+11343928 | None:intergenic |
| AAAGCTTTGTGTAACATCAT+TGG | 0.398015 | 1.3:-11343804 | MS.gene56057:CDS |
| TGACAGTAAAACACTGCATA+AGG | 0.401155 | 1.3:+11343620 | None:intergenic |
| TAAAGCTTACTCTGTGCCTT+TGG | 0.406987 | 1.3:-11343582 | MS.gene56057:CDS |
| AGCTTACTCTGTGCCTTTGG+AGG | 0.418522 | 1.3:-11343579 | MS.gene56057:CDS |
| TCAGGCAGGAGGTGACAAAC+TGG | 0.434507 | 1.3:+11343446 | None:intergenic |
| AGAAAGAAGAGTGCTACATT+TGG | 0.440436 | 1.3:+11344052 | None:intergenic |
| CAGCAATATACAATAGCATC+TGG | 0.447095 | 1.3:-11343698 | MS.gene56057:CDS |
| GTGCCTTTGGAGGGTGCTGA+TGG | 0.447611 | 1.3:-11343569 | MS.gene56057:CDS |
| TCCACCTTGTTTGATGAAAA+GGG | 0.448369 | 1.3:+11343929 | None:intergenic |
| AGAATGGAACCCAAAACATT+TGG | 0.452431 | 1.3:-11343504 | MS.gene56057:CDS |
| AGCACTCTTCTTTCTGGAAA+AGG | 0.453598 | 1.3:-11344044 | MS.gene56057:CDS |
| TCAAATGTAGCTTGTGGTAG+TGG | 0.457950 | 1.3:-11344919 | MS.gene56057:intron |
| CTGGAAAAGGACTTGCATCA+TGG | 0.459024 | 1.3:-11344031 | MS.gene56057:CDS |
| GATTTCACTACTTCAAAGCT+TGG | 0.470526 | 1.3:-11343770 | MS.gene56057:CDS |
| GCTTACTCTGTGCCTTTGGA+GGG | 0.480708 | 1.3:-11343578 | MS.gene56057:CDS |
| TGTGAAGAGCAAGGGATCAA+AGG | 0.490839 | 1.3:-11343833 | MS.gene56057:CDS |
| CAAGTACTCAAAGTTCAACC+TGG | 0.499411 | 1.3:-11343476 | MS.gene56057:CDS |
| GCATCTGGAGTGAAGAAGTT+GGG | 0.513820 | 1.3:-11343683 | MS.gene56057:CDS |
| ATCCAAACAACATGATCCTC+AGG | 0.517927 | 1.3:+11343428 | None:intergenic |
| GGAGTGAAGAGAAAGGAACT+TGG | 0.518251 | 1.3:-11344216 | MS.gene56057:CDS |
| GTCAGAAAGGGATACTATGA+AGG | 0.523139 | 1.3:-11344160 | MS.gene56057:CDS |
| GACAGTAAAACACTGCATAA+GGG | 0.527238 | 1.3:+11343621 | None:intergenic |
| GGCAAGGGTGGTGTAGATGT+TGG | 0.529745 | 1.3:-11344184 | MS.gene56057:CDS |
| AAAATCAAGAAAGGGTCAAA+GGG | 0.531552 | 1.3:-11343887 | MS.gene56057:CDS |
| AGCATCTGGAGTGAAGAAGT+TGG | 0.536857 | 1.3:-11343684 | MS.gene56057:CDS |
| TGTAGATGTTGGAGTCAGAA+AGG | 0.539383 | 1.3:-11344173 | MS.gene56057:CDS |
| CATTGAAGAGGTGGGAAGCA+TGG | 0.540494 | 1.3:+11344854 | None:intergenic |
| TGACAAACTGGAACAGTCCC+AGG | 0.541759 | 1.3:+11343458 | None:intergenic |
| AAAGGAACTTGGGTGGATGT+AGG | 0.554611 | 1.3:-11344205 | MS.gene56057:CDS |
| GGATATTGGAGTGAAGAGAA+AGG | 0.556237 | 1.3:-11344223 | MS.gene56057:CDS |
| AACTTGGGTGGATGTAGGCA+AGG | 0.557293 | 1.3:-11344200 | MS.gene56057:CDS |
| AGTTTGTCACCTCCTGCCTG+AGG | 0.562196 | 1.3:-11343444 | MS.gene56057:CDS |
| GTAGATGTTGGAGTCAGAAA+GGG | 0.562316 | 1.3:-11344172 | MS.gene56057:CDS |
| GTCAGTGAGTGTGAAGAGCA+AGG | 0.568006 | 1.3:-11343842 | MS.gene56057:CDS |
| CTTCCATCAGCACCCTCCAA+AGG | 0.576677 | 1.3:+11343566 | None:intergenic |
| TGAAGAGAAAGGAACTTGGG+TGG | 0.577433 | 1.3:-11344212 | MS.gene56057:CDS |
| TTGCTCTTCACACTCACTGA+CGG | 0.578201 | 1.3:+11343844 | None:intergenic |
| TGGGTGGATGTAGGCAAGGG+TGG | 0.579389 | 1.3:-11344196 | MS.gene56057:CDS |
| GAGTGAAGAGAAAGGAACTT+GGG | 0.579403 | 1.3:-11344215 | MS.gene56057:CDS |
| GGTGGCACTGATGTGAATGT+TGG | 0.587969 | 1.3:-11344136 | MS.gene56057:CDS |
| TCAGTGAGTGTGAAGAGCAA+GGG | 0.599484 | 1.3:-11343841 | MS.gene56057:CDS |
| AACTTGGCGCGGCAAGAATG+TGG | 0.603165 | 1.3:+11343964 | None:intergenic |
| GACTTCCAGTAAAGTTGAGG+AGG | 0.605659 | 1.3:+11344876 | None:intergenic |
| GTCACACTGATACATCAGAA+TGG | 0.606500 | 1.3:-11343520 | MS.gene56057:CDS |
| AAGGGATACTATGAAGGAGG+TGG | 0.607188 | 1.3:-11344154 | MS.gene56057:CDS |
| AAACAACATGATCCTCAGGC+AGG | 0.608017 | 1.3:+11343432 | None:intergenic |
| ATGGACTTCCAGTAAAGTTG+AGG | 0.616898 | 1.3:+11344873 | None:intergenic |
| AGGGTATTGAATTAGCAACT+TGG | 0.619732 | 1.3:+11343948 | None:intergenic |
| AAGTACTCAAAGTTCAACCT+GGG | 0.622856 | 1.3:-11343475 | MS.gene56057:CDS |
| AGGGTGTAGCAGATTAGTGA+TGG | 0.623388 | 1.3:+11344824 | None:intergenic |
| ATTGAATTAGCAACTTGGCG+CGG | 0.637675 | 1.3:+11343953 | None:intergenic |
| ACTGATGTGAATGTTGGAGT+TGG | 0.647770 | 1.3:-11344130 | MS.gene56057:CDS |
| CAACATGATCCTCAGGCAGG+AGG | 0.654087 | 1.3:+11343435 | None:intergenic |
| ACTTGGGTGGATGTAGGCAA+GGG | 0.659855 | 1.3:-11344199 | MS.gene56057:CDS |
| GAATGTGGCTGCATTTGAAG+TGG | 0.663147 | 1.3:+11343979 | None:intergenic |
| ATCACTAATCTGCTACACCC+TGG | 0.665412 | 1.3:-11344822 | MS.gene56057:intron |
| AGAGCAAGGGATCAAAGGAG+AGG | 0.677572 | 1.3:-11343828 | MS.gene56057:CDS |
| AGAAAGGGATACTATGAAGG+AGG | 0.679610 | 1.3:-11344157 | MS.gene56057:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAATAAATAAAAAGTCTA+TGG | - | chr1.3:11344153-11344172 | MS.gene56057:CDS | 10.0% |
| !! | AAATAAATAAACCAAGTAAT+TGG | - | chr1.3:11344019-11344038 | MS.gene56057:CDS | 15.0% |
| !! | AATAAATAAACCAAGTAATT+GGG | - | chr1.3:11344020-11344039 | MS.gene56057:CDS | 15.0% |
| !! | GTTAATTATTTGAAGAAAAT+TGG | + | chr1.3:11343485-11343504 | None:intergenic | 15.0% |
| !!! | ATACTCATTTTATATATAGA+TGG | - | chr1.3:11343717-11343736 | MS.gene56057:CDS | 15.0% |
| !!! | GTTTAAAATGATTTCATTTT+AGG | - | chr1.3:11343756-11343775 | MS.gene56057:CDS | 15.0% |
| !! | AACAAGTTAAAAATCAAGAA+AGG | - | chr1.3:11344571-11344590 | MS.gene56057:intron | 20.0% |
| !! | ACAAGTTAAAAATCAAGAAA+GGG | - | chr1.3:11344572-11344591 | MS.gene56057:intron | 20.0% |
| !! | ACCATGAAAAAATTAATGAT+GGG | + | chr1.3:11343439-11343458 | None:intergenic | 20.0% |
| !! | GCAGCATAATTATAAATAAA+TGG | + | chr1.3:11344370-11344389 | None:intergenic | 20.0% |
| !!! | ATGAAATCATTTTAAACACA+TGG | + | chr1.3:11343753-11343772 | None:intergenic | 20.0% |
| ! | AAAGGACTTATGATATATGA+AGG | - | chr1.3:11343986-11344005 | MS.gene56057:CDS | 25.0% |
| ! | ATTTAATTCATGCACATTGT+CGG | - | chr1.3:11343779-11343798 | MS.gene56057:CDS | 25.0% |
| ! | CACCATGAAAAAATTAATGA+TGG | + | chr1.3:11343440-11343459 | None:intergenic | 25.0% |
| !!! | TATTTTTTGTGTTAACTCTC+CGG | + | chr1.3:11344133-11344152 | None:intergenic | 25.0% |
| !!! | TCCCATCATTAATTTTTTCA+TGG | - | chr1.3:11343435-11343454 | MS.gene56057:CDS | 25.0% |
| AAAAATCAAGAAAGGGTCAA+AGG | - | chr1.3:11344579-11344598 | MS.gene56057:intron | 30.0% | |
| AAAATCAAGAAAGGGTCAAA+GGG | - | chr1.3:11344580-11344599 | MS.gene56057:intron | 30.0% | |
| AAAGCTTGGAAAAAATGTTG+AGG | - | chr1.3:11344711-11344730 | MS.gene56057:intron | 30.0% | |
| TTGAATTATTCGTCATAGAG+AGG | + | chr1.3:11344063-11344082 | None:intergenic | 30.0% | |
| ! | AAAGCTTTGTGTAACATCAT+TGG | - | chr1.3:11344663-11344682 | MS.gene56057:intron | 30.0% |
| ! | AATACCCTTTTCATCAAACA+AGG | - | chr1.3:11344534-11344553 | MS.gene56057:intron | 30.0% |
| ! | ACTTGAAATGCCAAATGTTT+TGG | + | chr1.3:11344976-11344995 | None:intergenic | 30.0% |
| ! | ATTTATCTGAAGCTGGATAT+TGG | - | chr1.3:11344230-11344249 | MS.gene56057:intron | 30.0% |
| ! | CTTGAAATGCCAAATGTTTT+GGG | + | chr1.3:11344975-11344994 | None:intergenic | 30.0% |
| ! | TTACTCTATTTATCTGAAGC+TGG | - | chr1.3:11344223-11344242 | MS.gene56057:CDS | 30.0% |
| !!! | ATTTTTTGTGTTAACTCTCC+GGG | + | chr1.3:11344132-11344151 | None:intergenic | 30.0% |
| AAATGTAGCACTCTTCTTTC+TGG | - | chr1.3:11344417-11344436 | MS.gene56057:intron | 35.0% | |
| AAGTACTCAAAGTTCAACCT+GGG | - | chr1.3:11344992-11345011 | MS.gene56057:intron | 35.0% | |
| ACCAAGTAATTGGGTTTAAG+TGG | - | chr1.3:11344029-11344048 | MS.gene56057:CDS | 35.0% | |
| ACCACTTAAACCCAATTACT+TGG | + | chr1.3:11344033-11344052 | None:intergenic | 35.0% | |
| AGAATGGAACCCAAAACATT+TGG | - | chr1.3:11344963-11344982 | MS.gene56057:intron | 35.0% | |
| CAGCAATATACAATAGCATC+TGG | - | chr1.3:11344769-11344788 | MS.gene56057:intron | 35.0% | |
| CTAGACTATGATACTAACCA+GGG | + | chr1.3:11343665-11343684 | None:intergenic | 35.0% | |
| CTATTCTCAAATGTAGCTTG+TGG | - | chr1.3:11343542-11343561 | MS.gene56057:CDS | 35.0% | |
| GACAGTAAAACACTGCATAA+GGG | + | chr1.3:11344849-11344868 | None:intergenic | 35.0% | |
| GATTTCACTACTTCAAAGCT+TGG | - | chr1.3:11344697-11344716 | MS.gene56057:intron | 35.0% | |
| TCAGATGATCTCTATCAGAA+AGG | - | chr1.3:11343968-11343987 | MS.gene56057:CDS | 35.0% | |
| TCTAGACTATGATACTAACC+AGG | + | chr1.3:11343666-11343685 | None:intergenic | 35.0% | |
| TGACAGTAAAACACTGCATA+AGG | + | chr1.3:11344850-11344869 | None:intergenic | 35.0% | |
| TGTAACATCATTGGAGTCTA+TGG | - | chr1.3:11344672-11344691 | MS.gene56057:intron | 35.0% | |
| ! | TCCACCTTGTTTGATGAAAA+GGG | + | chr1.3:11344541-11344560 | None:intergenic | 35.0% |
| ! | TTCCACCTTGTTTGATGAAA+AGG | + | chr1.3:11344542-11344561 | None:intergenic | 35.0% |
| !! | AGAAAGAAGAGTGCTACATT+TGG | + | chr1.3:11344418-11344437 | None:intergenic | 35.0% |
| !! | AGGGTATTGAATTAGCAACT+TGG | + | chr1.3:11344522-11344541 | None:intergenic | 35.0% |
| !!! | TTTTTTGTGTTAACTCTCCG+GGG | + | chr1.3:11344131-11344150 | None:intergenic | 35.0% |
| ACTGATGTGAATGTTGGAGT+TGG | - | chr1.3:11344337-11344356 | MS.gene56057:intron | 40.0% | |
| AGAAAGGGATACTATGAAGG+AGG | - | chr1.3:11344310-11344329 | MS.gene56057:intron | 40.0% | |
| ATCCAAACAACATGATCCTC+AGG | + | chr1.3:11345042-11345061 | None:intergenic | 40.0% | |
| ATGGACTTCCAGTAAAGTTG+AGG | + | chr1.3:11343597-11343616 | None:intergenic | 40.0% | |
| ATTGAATTAGCAACTTGGCG+CGG | + | chr1.3:11344517-11344536 | None:intergenic | 40.0% | |
| CAAGTACTCAAAGTTCAACC+TGG | - | chr1.3:11344991-11345010 | MS.gene56057:intron | 40.0% | |
| GAGTGAAGAGAAAGGAACTT+GGG | - | chr1.3:11344252-11344271 | MS.gene56057:intron | 40.0% | |
| GGATATTGGAGTGAAGAGAA+AGG | - | chr1.3:11344244-11344263 | MS.gene56057:intron | 40.0% | |
| GTAGATGTTGGAGTCAGAAA+GGG | - | chr1.3:11344295-11344314 | MS.gene56057:intron | 40.0% | |
| GTCACACTGATACATCAGAA+TGG | - | chr1.3:11344947-11344966 | MS.gene56057:intron | 40.0% | |
| GTCAGAAAGGGATACTATGA+AGG | - | chr1.3:11344307-11344326 | MS.gene56057:intron | 40.0% | |
| TAAAGCTTACTCTGTGCCTT+TGG | - | chr1.3:11344885-11344904 | MS.gene56057:CDS | 40.0% | |
| TCTTACCTCCTCAACTTTAC+TGG | - | chr1.3:11343586-11343605 | MS.gene56057:CDS | 40.0% | |
| TGTAGATGTTGGAGTCAGAA+AGG | - | chr1.3:11344294-11344313 | MS.gene56057:intron | 40.0% | |
| ! | ACCCTTTTCATCAAACAAGG+TGG | - | chr1.3:11344537-11344556 | MS.gene56057:intron | 40.0% |
| ! | AGCACTCTTCTTTCTGGAAA+AGG | - | chr1.3:11344423-11344442 | MS.gene56057:intron | 40.0% |
| ! | AGCAGATTAGTGATGGCTTT+TGG | + | chr1.3:11343639-11343658 | None:intergenic | 40.0% |
| ! | TCAAATGTAGCTTGTGGTAG+TGG | - | chr1.3:11343548-11343567 | MS.gene56057:CDS | 40.0% |
| AAACAACATGATCCTCAGGC+AGG | + | chr1.3:11345038-11345057 | None:intergenic | 45.0% | |
| AAAGGAACTTGGGTGGATGT+AGG | - | chr1.3:11344262-11344281 | MS.gene56057:intron | 45.0% | |
| AAGGGATACTATGAAGGAGG+TGG | - | chr1.3:11344313-11344332 | MS.gene56057:intron | 45.0% | |
| AGGGTGTAGCAGATTAGTGA+TGG | + | chr1.3:11343646-11343665 | None:intergenic | 45.0% | |
| ATCACTAATCTGCTACACCC+TGG | - | chr1.3:11343645-11343664 | MS.gene56057:CDS | 45.0% | |
| CTGGAAAAGGACTTGCATCA+TGG | - | chr1.3:11344436-11344455 | MS.gene56057:intron | 45.0% | |
| GAATGTGGCTGCATTTGAAG+TGG | + | chr1.3:11344491-11344510 | None:intergenic | 45.0% | |
| GACTTCCAGTAAAGTTGAGG+AGG | + | chr1.3:11343594-11343613 | None:intergenic | 45.0% | |
| GGAGTGAAGAGAAAGGAACT+TGG | - | chr1.3:11344251-11344270 | MS.gene56057:intron | 45.0% | |
| TCAGTGAGTGTGAAGAGCAA+GGG | - | chr1.3:11344626-11344645 | MS.gene56057:intron | 45.0% | |
| TGAAGAGAAAGGAACTTGGG+TGG | - | chr1.3:11344255-11344274 | MS.gene56057:intron | 45.0% | |
| TGCCTGAGGATCATGTTGTT+TGG | - | chr1.3:11345037-11345056 | MS.gene56057:CDS | 45.0% | |
| TGTGAAGAGCAAGGGATCAA+AGG | - | chr1.3:11344634-11344653 | MS.gene56057:intron | 45.0% | |
| TTGCTCTTCACACTCACTGA+CGG | + | chr1.3:11344626-11344645 | None:intergenic | 45.0% | |
| ! | AGCATCTGGAGTGAAGAAGT+TGG | - | chr1.3:11344783-11344802 | MS.gene56057:intron | 45.0% |
| ! | GCATCTGGAGTGAAGAAGTT+GGG | - | chr1.3:11344784-11344803 | MS.gene56057:intron | 45.0% |
| !! | GATGGCTTTTGGCATTGAAG+AGG | + | chr1.3:11343628-11343647 | None:intergenic | 45.0% |
| !! | GCTTTTGGCATTGAAGAGGT+GGG | + | chr1.3:11343624-11343643 | None:intergenic | 45.0% |
| AACTTGGGTGGATGTAGGCA+AGG | - | chr1.3:11344267-11344286 | MS.gene56057:intron | 50.0% | |
| ACTTGGGTGGATGTAGGCAA+GGG | - | chr1.3:11344268-11344287 | MS.gene56057:intron | 50.0% | |
| AGAGCAAGGGATCAAAGGAG+AGG | - | chr1.3:11344639-11344658 | MS.gene56057:intron | 50.0% | |
| AGCTTACTCTGTGCCTTTGG+AGG | - | chr1.3:11344888-11344907 | MS.gene56057:CDS | 50.0% | |
| CATTGAAGAGGTGGGAAGCA+TGG | + | chr1.3:11343616-11343635 | None:intergenic | 50.0% | |
| GCTTACTCTGTGCCTTTGGA+GGG | - | chr1.3:11344889-11344908 | MS.gene56057:CDS | 50.0% | |
| GGAAGGGGGTCATAGTAATC+AGG | + | chr1.3:11344101-11344120 | None:intergenic | 50.0% | |
| GTCAGTGAGTGTGAAGAGCA+AGG | - | chr1.3:11344625-11344644 | MS.gene56057:intron | 50.0% | |
| GTTAACTCTCCGGGGTCTAA+GGG | + | chr1.3:11344123-11344142 | None:intergenic | 50.0% | |
| TGACAAACTGGAACAGTCCC+AGG | + | chr1.3:11345012-11345031 | None:intergenic | 50.0% | |
| TGTTAACTCTCCGGGGTCTA+AGG | + | chr1.3:11344124-11344143 | None:intergenic | 50.0% | |
| TTAACTCTCCGGGGTCTAAG+GGG | + | chr1.3:11344122-11344141 | None:intergenic | 50.0% | |
| !! | GGCTTTTGGCATTGAAGAGG+TGG | + | chr1.3:11343625-11343644 | None:intergenic | 50.0% |
| !! | GGTGGCACTGATGTGAATGT+TGG | - | chr1.3:11344331-11344350 | MS.gene56057:intron | 50.0% |
| AACTTGGCGCGGCAAGAATG+TGG | + | chr1.3:11344506-11344525 | None:intergenic | 55.0% | |
| AGTTTGTCACCTCCTGCCTG+AGG | - | chr1.3:11345023-11345042 | MS.gene56057:intron | 55.0% | |
| CAACATGATCCTCAGGCAGG+AGG | + | chr1.3:11345035-11345054 | None:intergenic | 55.0% | |
| CTTCCATCAGCACCCTCCAA+AGG | + | chr1.3:11344904-11344923 | None:intergenic | 55.0% | |
| GGCAAGGGTGGTGTAGATGT+TGG | - | chr1.3:11344283-11344302 | MS.gene56057:intron | 55.0% | |
| TCAGGCAGGAGGTGACAAAC+TGG | + | chr1.3:11345024-11345043 | None:intergenic | 55.0% | |
| TCTCCGGGGTCTAAGGGGAA+GGG | + | chr1.3:11344117-11344136 | None:intergenic | 60.0% | |
| TGGGTGGATGTAGGCAAGGG+TGG | - | chr1.3:11344271-11344290 | MS.gene56057:intron | 60.0% | |
| !! | GTGCCTTTGGAGGGTGCTGA+TGG | - | chr1.3:11344898-11344917 | MS.gene56057:CDS | 60.0% |
| ACCCCCTTCCCCTTAGACCC+CGG | - | chr1.3:11344111-11344130 | MS.gene56057:CDS | 65.0% | |
| CTCCGGGGTCTAAGGGGAAG+GGG | + | chr1.3:11344116-11344135 | None:intergenic | 65.0% | |
| CTCTCCGGGGTCTAAGGGGA+AGG | + | chr1.3:11344118-11344137 | None:intergenic | 65.0% | |
| TCCGGGGTCTAAGGGGAAGG+GGG | + | chr1.3:11344115-11344134 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 11343418 | 11345071 | 11343418 | ID=MS.gene56057 |
| chr1.3 | mRNA | 11343418 | 11345071 | 11343418 | ID=MS.gene56057.t1;Parent=MS.gene56057 |
| chr1.3 | exon | 11345033 | 11345071 | 11345033 | ID=MS.gene56057.t1.exon1;Parent=MS.gene56057.t1 |
| chr1.3 | CDS | 11345033 | 11345071 | 11345033 | ID=cds.MS.gene56057.t1;Parent=MS.gene56057.t1 |
| chr1.3 | exon | 11344823 | 11344931 | 11344823 | ID=MS.gene56057.t1.exon2;Parent=MS.gene56057.t1 |
| chr1.3 | CDS | 11344823 | 11344931 | 11344823 | ID=cds.MS.gene56057.t1;Parent=MS.gene56057.t1 |
| chr1.3 | exon | 11343418 | 11344247 | 11343418 | ID=MS.gene56057.t1.exon3;Parent=MS.gene56057.t1 |
| chr1.3 | CDS | 11343418 | 11344247 | 11343418 | ID=cds.MS.gene56057.t1;Parent=MS.gene56057.t1 |
| Gene Sequence |
| Protein sequence |
