Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64069.t1 | XP_003615156.1 | 80 | 150 | 8 | 1 | 1 | 128 | 1 | 150 | 4.00E-06 | 61.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64069.t1 | G7KAG0 | 80.0 | 150 | 8 | 1 | 1 | 128 | 1 | 150 | 2.8e-06 | 61.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049611 | MS.gene64069 | 0.827717 | 1.34E-54 | -1.69E-46 |
| MS.gene050082 | MS.gene64069 | 0.995149 | 1.78E-213 | -1.69E-46 |
| MS.gene050170 | MS.gene64069 | -0.811028 | 8.59E-51 | -1.69E-46 |
| MS.gene050323 | MS.gene64069 | -0.811136 | 8.14E-51 | -1.69E-46 |
| MS.gene050333 | MS.gene64069 | -0.805045 | 1.61E-49 | -1.69E-46 |
| MS.gene050338 | MS.gene64069 | 0.81753 | 3.14E-52 | -1.69E-46 |
| MS.gene050381 | MS.gene64069 | 0.80921 | 2.12E-50 | -1.69E-46 |
| MS.gene050496 | MS.gene64069 | -0.829207 | 5.86E-55 | -1.69E-46 |
| MS.gene050612 | MS.gene64069 | -0.813106 | 3.03E-51 | -1.69E-46 |
| MS.gene051138 | MS.gene64069 | 0.841375 | 4.94E-58 | -1.69E-46 |
| MS.gene051364 | MS.gene64069 | 0.841488 | 4.61E-58 | -1.69E-46 |
| MS.gene051366 | MS.gene64069 | 0.83368 | 4.64E-56 | -1.69E-46 |
| MS.gene051367 | MS.gene64069 | 0.852287 | 5.10E-61 | -1.69E-46 |
| MS.gene051422 | MS.gene64069 | 0.833624 | 4.79E-56 | -1.69E-46 |
| MS.gene051651 | MS.gene64069 | 0.808951 | 2.41E-50 | -1.69E-46 |
| MS.gene051718 | MS.gene64069 | 0.800016 | 1.76E-48 | -1.69E-46 |
| MS.gene052486 | MS.gene64069 | -0.800066 | 1.72E-48 | -1.69E-46 |
| MS.gene053420 | MS.gene64069 | 0.850612 | 1.52E-60 | -1.69E-46 |
| MS.gene053568 | MS.gene64069 | 0.883838 | 3.20E-71 | -1.69E-46 |
| MS.gene053569 | MS.gene64069 | 0.858132 | 1.02E-62 | -1.69E-46 |
| MS.gene055158 | MS.gene64069 | 0.809814 | 1.57E-50 | -1.69E-46 |
| MS.gene055510 | MS.gene64069 | 0.812281 | 4.59E-51 | -1.69E-46 |
| MS.gene055751 | MS.gene64069 | -0.80601 | 1.01E-49 | -1.69E-46 |
| MS.gene05589 | MS.gene64069 | -0.821736 | 3.45E-53 | -1.69E-46 |
| MS.gene05590 | MS.gene64069 | 0.814477 | 1.51E-51 | -1.69E-46 |
| MS.gene056198 | MS.gene64069 | 0.817353 | 3.45E-52 | -1.69E-46 |
| MS.gene056387 | MS.gene64069 | -0.800607 | 1.33E-48 | -1.69E-46 |
| MS.gene056504 | MS.gene64069 | -0.837609 | 4.70E-57 | -1.69E-46 |
| MS.gene056692 | MS.gene64069 | -0.809802 | 1.58E-50 | -1.69E-46 |
| MS.gene056856 | MS.gene64069 | 0.84197 | 3.44E-58 | -1.69E-46 |
| MS.gene057549 | MS.gene64069 | 0.808419 | 3.13E-50 | -1.69E-46 |
| MS.gene057717 | MS.gene64069 | 0.820198 | 7.79E-53 | -1.69E-46 |
| MS.gene058067 | MS.gene64069 | 0.83473 | 2.53E-56 | -1.69E-46 |
| MS.gene058068 | MS.gene64069 | 0.881703 | 1.93E-70 | -1.69E-46 |
| MS.gene058099 | MS.gene64069 | 0.824089 | 9.75E-54 | -1.69E-46 |
| MS.gene058111 | MS.gene64069 | 0.826658 | 2.40E-54 | -1.69E-46 |
| MS.gene058157 | MS.gene64069 | -0.808872 | 2.50E-50 | -1.69E-46 |
| MS.gene058171 | MS.gene64069 | 0.822558 | 2.22E-53 | -1.69E-46 |
| MS.gene058173 | MS.gene64069 | 0.822808 | 1.94E-53 | -1.69E-46 |
| MS.gene058175 | MS.gene64069 | 0.826471 | 2.66E-54 | -1.69E-46 |
| MS.gene058268 | MS.gene64069 | 0.838454 | 2.85E-57 | -1.69E-46 |
| MS.gene058269 | MS.gene64069 | 0.829872 | 4.04E-55 | -1.69E-46 |
| MS.gene058382 | MS.gene64069 | 0.804355 | 2.25E-49 | -1.69E-46 |
| MS.gene058493 | MS.gene64069 | 0.802766 | 4.80E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene64069 | -0.806225 | 9.12E-50 | -1.69E-46 |
| MS.gene059070 | MS.gene64069 | 0.806881 | 6.63E-50 | -1.69E-46 |
| MS.gene059685 | MS.gene64069 | 0.816733 | 4.75E-52 | -1.69E-46 |
| MS.gene059865 | MS.gene64069 | -0.8233 | 1.49E-53 | -1.69E-46 |
| MS.gene060595 | MS.gene64069 | 0.821748 | 3.42E-53 | -1.69E-46 |
| MS.gene060762 | MS.gene64069 | -0.812013 | 5.25E-51 | -1.69E-46 |
| MS.gene060778 | MS.gene64069 | -0.805861 | 1.09E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene64069 | -0.801653 | 8.14E-49 | -1.69E-46 |
| MS.gene060823 | MS.gene64069 | 0.834602 | 2.73E-56 | -1.69E-46 |
| MS.gene060982 | MS.gene64069 | -0.810725 | 9.99E-51 | -1.69E-46 |
| MS.gene061044 | MS.gene64069 | 0.817129 | 3.87E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64069.t1 | MTR_5g064530 | 80.000 | 150 | 8 | 1 | 1 | 128 | 1 | 150 | 2.35e-58 | 177 |
| MS.gene64069.t1 | MTR_5g064510 | 78.512 | 121 | 10 | 1 | 1 | 105 | 1 | 121 | 1.09e-49 | 155 |
| MS.gene64069.t1 | MTR_4g093910 | 72.566 | 113 | 27 | 3 | 1 | 111 | 1 | 111 | 3.45e-32 | 110 |
| MS.gene64069.t1 | MTR_4g093950 | 72.566 | 113 | 27 | 3 | 1 | 111 | 1 | 111 | 5.10e-32 | 110 |
| MS.gene64069.t1 | MTR_4g093940 | 72.072 | 111 | 27 | 3 | 1 | 109 | 1 | 109 | 6.62e-32 | 109 |
| MS.gene64069.t1 | MTR_4g093920 | 72.072 | 111 | 26 | 4 | 1 | 109 | 1 | 108 | 1.69e-29 | 103 |
| MS.gene64069.t1 | MTR_4g094678 | 60.494 | 81 | 11 | 5 | 17 | 93 | 1 | 64 | 2.33e-14 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGGGAGTGTTGGATTAGT+AGG | 0.289744 | 5.4:-50264075 | None:intergenic |
| GAAAGGGATGGAGGAGATAA+TGG | 0.324144 | 5.4:-50264154 | None:intergenic |
| ATTGAAGGCAATGTTGGTTT+TGG | 0.363932 | 5.4:-50263931 | None:intergenic |
| TGAGGCAATGTTGGAATAGA+AGG | 0.409708 | 5.4:-50263976 | None:intergenic |
| GGGGTAGAGGTTGAAGGTGG+TGG | 0.414326 | 5.4:-50264183 | None:intergenic |
| AGTGAATTGGTTGGCAATGG+TGG | 0.426352 | 5.4:-50264108 | None:intergenic |
| GGAACATTGCCTTGAGGTTG+AGG | 0.447102 | 5.4:-50263994 | None:intergenic |
| AGGAATGTTGGGAAGTGAAT+TGG | 0.464436 | 5.4:-50264121 | None:intergenic |
| GAAGGTGGTGGAGAGAAGAA+AGG | 0.468019 | 5.4:-50264171 | None:intergenic |
| GGTTGAGATTGAAGGAATGT+TGG | 0.470769 | 5.4:-50264133 | None:intergenic |
| GTTGGATTAGTAGGAATGCT+AGG | 0.470882 | 5.4:-50264066 | None:intergenic |
| GGCAAAGGTGGCAATGTTGT+TGG | 0.474544 | 5.4:-50263955 | None:intergenic |
| GGCAATGTTGTTGGAATTGA+AGG | 0.477009 | 5.4:-50263946 | None:intergenic |
| GTTGTGTGGTTGTTTGCAAA+AGG | 0.480622 | 5.4:-50263900 | None:intergenic |
| CCTTGAGGTTGAGGCAATGT+TGG | 0.482455 | 5.4:-50263985 | None:intergenic |
| GAGATAATGGTTGAGATTGA+AGG | 0.488067 | 5.4:-50264141 | None:intergenic |
| AAGGTGGTGGAGAGAAGAAA+GGG | 0.496086 | 5.4:-50264170 | None:intergenic |
| ATGGTGGAAAGTTGAGAGAT+GGG | 0.499488 | 5.4:-50264092 | None:intergenic |
| GTTGGAATTGAAGGCAATGT+TGG | 0.501730 | 5.4:-50263937 | None:intergenic |
| GTTGAGATTGAAGGAATGTT+GGG | 0.510751 | 5.4:-50264132 | None:intergenic |
| TGGAGAGAAGAAAGGGATGG+AGG | 0.512326 | 5.4:-50264163 | None:intergenic |
| AAGAAGTAAAGATGTGATGA+TGG | 0.515143 | 5.4:-50263839 | None:intergenic |
| CTTCAAGGGGTAGAGGTTGA+AGG | 0.516901 | 5.4:-50264189 | None:intergenic |
| GGAAGTGAATTGGTTGGCAA+TGG | 0.525163 | 5.4:-50264111 | None:intergenic |
| AGTGGTGGCATAGTGAGCTT+AGG | 0.545367 | 5.4:-50264042 | None:intergenic |
| TGGTGGAGAGAAGAAAGGGA+TGG | 0.548047 | 5.4:-50264166 | None:intergenic |
| AAGTTGAGAGATGGGAGTGT+TGG | 0.551170 | 5.4:-50264084 | None:intergenic |
| ATAGTGAGCTTAGGCACTGA+AGG | 0.554532 | 5.4:-50264033 | None:intergenic |
| TTAGTAGGAATGCTAGGAAG+TGG | 0.565420 | 5.4:-50264060 | None:intergenic |
| GAGCTTAGGCACTGAAGGGA+TGG | 0.575148 | 5.4:-50264028 | None:intergenic |
| TTAGGCACTGAAGGGATGGT+AGG | 0.587385 | 5.4:-50264024 | None:intergenic |
| TTCTATGCTCATGTTTGACA+AGG | 0.589337 | 5.4:-50263869 | None:intergenic |
| AATGGTGGAAAGTTGAGAGA+TGG | 0.590990 | 5.4:-50264093 | None:intergenic |
| GAAGGGATGGTAGGCAATGG+AGG | 0.598281 | 5.4:-50264015 | None:intergenic |
| GTTGGAATAGAAGGCAAAGG+TGG | 0.615611 | 5.4:-50263967 | None:intergenic |
| CCAACATTGCCTCAACCTCA+AGG | 0.620333 | 5.4:+50263985 | MS.gene64069:CDS |
| TAGTGAGCTTAGGCACTGAA+GGG | 0.628071 | 5.4:-50264032 | None:intergenic |
| GTAGGAATGCTAGGAAGTGG+TGG | 0.647666 | 5.4:-50264057 | None:intergenic |
| ACTGAAGGGATGGTAGGCAA+TGG | 0.655830 | 5.4:-50264018 | None:intergenic |
| CAAGGGGTAGAGGTTGAAGG+TGG | 0.657058 | 5.4:-50264186 | None:intergenic |
| AATGTTGGAATAGAAGGCAA+AGG | 0.665937 | 5.4:-50263970 | None:intergenic |
| AATGGAGGAACATTGCCTTG+AGG | 0.697803 | 5.4:-50264000 | None:intergenic |
| ATGTTGGGAAGTGAATTGGT+TGG | 0.737713 | 5.4:-50264117 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGAAGTAAAGATGTGATGA+TGG | - | chr5.4:50263842-50263861 | None:intergenic | 30.0% | |
| !!! | AATGTTGGTTTTGGCAAATT+GGG | - | chr5.4:50263925-50263944 | None:intergenic | 30.0% |
| AATGTTGGAATAGAAGGCAA+AGG | - | chr5.4:50263973-50263992 | None:intergenic | 35.0% | |
| GTTGAGATTGAAGGAATGTT+GGG | - | chr5.4:50264135-50264154 | None:intergenic | 35.0% | |
| TTCTATGCTCATGTTTGACA+AGG | - | chr5.4:50263872-50263891 | None:intergenic | 35.0% | |
| ! | GAGATAATGGTTGAGATTGA+AGG | - | chr5.4:50264144-50264163 | None:intergenic | 35.0% |
| !!! | ATTGAAGGCAATGTTGGTTT+TGG | - | chr5.4:50263934-50263953 | None:intergenic | 35.0% |
| !!! | CAATGTTGGTTTTGGCAAAT+TGG | - | chr5.4:50263926-50263945 | None:intergenic | 35.0% |
| !!! | TGTGATGATGGTTTTCATTG+AGG | - | chr5.4:50263830-50263849 | None:intergenic | 35.0% |
| AGGAATGTTGGGAAGTGAAT+TGG | - | chr5.4:50264124-50264143 | None:intergenic | 40.0% | |
| ATGTTGGGAAGTGAATTGGT+TGG | - | chr5.4:50264120-50264139 | None:intergenic | 40.0% | |
| GGCAATGTTGTTGGAATTGA+AGG | - | chr5.4:50263949-50263968 | None:intergenic | 40.0% | |
| GGTTGAGATTGAAGGAATGT+TGG | - | chr5.4:50264136-50264155 | None:intergenic | 40.0% | |
| GTTGGAATTGAAGGCAATGT+TGG | - | chr5.4:50263940-50263959 | None:intergenic | 40.0% | |
| GTTGTGTGGTTGTTTGCAAA+AGG | - | chr5.4:50263903-50263922 | None:intergenic | 40.0% | |
| TGAGGCAATGTTGGAATAGA+AGG | - | chr5.4:50263979-50263998 | None:intergenic | 40.0% | |
| TTAGTAGGAATGCTAGGAAG+TGG | - | chr5.4:50264063-50264082 | None:intergenic | 40.0% | |
| ! | AATGGTGGAAAGTTGAGAGA+TGG | - | chr5.4:50264096-50264115 | None:intergenic | 40.0% |
| ! | ATGGTGGAAAGTTGAGAGAT+GGG | - | chr5.4:50264095-50264114 | None:intergenic | 40.0% |
| !! | GTTGGATTAGTAGGAATGCT+AGG | - | chr5.4:50264069-50264088 | None:intergenic | 40.0% |
| !! | TTTTGGCAAATTGGGTTGTG+TGG | - | chr5.4:50263917-50263936 | None:intergenic | 40.0% |
| AATGGAGGAACATTGCCTTG+AGG | - | chr5.4:50264003-50264022 | None:intergenic | 45.0% | |
| AGTGAATTGGTTGGCAATGG+TGG | - | chr5.4:50264111-50264130 | None:intergenic | 45.0% | |
| GAAAGGGATGGAGGAGATAA+TGG | - | chr5.4:50264157-50264176 | None:intergenic | 45.0% | |
| GGAAGTGAATTGGTTGGCAA+TGG | - | chr5.4:50264114-50264133 | None:intergenic | 45.0% | |
| GTTGGAATAGAAGGCAAAGG+TGG | - | chr5.4:50263970-50263989 | None:intergenic | 45.0% | |
| ! | AAGTTGAGAGATGGGAGTGT+TGG | - | chr5.4:50264087-50264106 | None:intergenic | 45.0% |
| !! | AAGGTGGTGGAGAGAAGAAA+GGG | - | chr5.4:50264173-50264192 | None:intergenic | 45.0% |
| !! | ATAGTGAGCTTAGGCACTGA+AGG | - | chr5.4:50264036-50264055 | None:intergenic | 45.0% |
| !! | GATGGGAGTGTTGGATTAGT+AGG | - | chr5.4:50264078-50264097 | None:intergenic | 45.0% |
| !! | TAGTGAGCTTAGGCACTGAA+GGG | - | chr5.4:50264035-50264054 | None:intergenic | 45.0% |
| ACTGAAGGGATGGTAGGCAA+TGG | - | chr5.4:50264021-50264040 | None:intergenic | 50.0% | |
| AGTGGTGGCATAGTGAGCTT+AGG | - | chr5.4:50264045-50264064 | None:intergenic | 50.0% | |
| CCAACATTGCCTCAACCTCA+AGG | + | chr5.4:50263985-50264004 | MS.gene64069:CDS | 50.0% | |
| CCTTGAGGTTGAGGCAATGT+TGG | - | chr5.4:50263988-50264007 | None:intergenic | 50.0% | |
| GGAACATTGCCTTGAGGTTG+AGG | - | chr5.4:50263997-50264016 | None:intergenic | 50.0% | |
| GGCAAAGGTGGCAATGTTGT+TGG | - | chr5.4:50263958-50263977 | None:intergenic | 50.0% | |
| GTAGGAATGCTAGGAAGTGG+TGG | - | chr5.4:50264060-50264079 | None:intergenic | 50.0% | |
| TGGAGAGAAGAAAGGGATGG+AGG | - | chr5.4:50264166-50264185 | None:intergenic | 50.0% | |
| TGGTGGAGAGAAGAAAGGGA+TGG | - | chr5.4:50264169-50264188 | None:intergenic | 50.0% | |
| !! | GAAGGTGGTGGAGAGAAGAA+AGG | - | chr5.4:50264174-50264193 | None:intergenic | 50.0% |
| !! | TTAGGCACTGAAGGGATGGT+AGG | - | chr5.4:50264027-50264046 | None:intergenic | 50.0% |
| CAAGGGGTAGAGGTTGAAGG+TGG | - | chr5.4:50264189-50264208 | None:intergenic | 55.0% | |
| GAAGGGATGGTAGGCAATGG+AGG | - | chr5.4:50264018-50264037 | None:intergenic | 55.0% | |
| !! | GAGCTTAGGCACTGAAGGGA+TGG | - | chr5.4:50264031-50264050 | None:intergenic | 55.0% |
| GGGGTAGAGGTTGAAGGTGG+TGG | - | chr5.4:50264186-50264205 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 50263823 | 50264209 | 50263823 | ID=MS.gene64069 |
| chr5.4 | mRNA | 50263823 | 50264209 | 50263823 | ID=MS.gene64069.t1;Parent=MS.gene64069 |
| chr5.4 | exon | 50263823 | 50264209 | 50263823 | ID=MS.gene64069.t1.exon1;Parent=MS.gene64069.t1 |
| chr5.4 | CDS | 50263823 | 50264209 | 50263823 | ID=cds.MS.gene64069.t1;Parent=MS.gene64069.t1 |
| Gene Sequence |
| Protein sequence |
