AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39959


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39959.t1 MTR_1g071720 94.828 116 6 0 1 116 75 190 2.36e-76 224
MS.gene39959.t1 MTR_1g071720 93.966 116 6 1 1 116 75 189 8.96e-74 217
MS.gene39959.t1 MTR_5g006940 52.874 87 37 2 3 89 76 158 6.16e-19 78.6
MS.gene39959.t1 MTR_1g103420 38.261 115 47 3 1 94 74 185 1.12e-16 72.8
MS.gene39959.t1 MTR_7g095250 40.741 108 52 4 1 107 39 135 1.78e-15 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39959.t1 AT2G48140 52.459 61 28 1 1 61 68 127 4.38e-14 63.9
MS.gene39959.t1 AT2G48140 56.863 51 22 0 1 51 68 118 7.11e-14 65.1
MS.gene39959.t1 AT1G05450 55.357 56 24 1 1 56 71 125 1.44e-13 64.3

Find 31 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGCAGGTCCAGCTATATT+TGG 0.239605 1.4:-58669471 MS.gene39959:intron
GAAGGAAAGAACTCTATTAA+TGG 0.297936 1.4:+58670986 None:intergenic
GAGTGTTGAATTGCATATTT+AGG 0.320907 1.4:+58669230 None:intergenic
CTTGGGCGACTATGCTTCAT+TGG 0.351622 1.4:+58669176 None:intergenic
CTCAAGGGAGAAGCATTAAT+TGG 0.353542 1.4:+58669426 None:intergenic
TTAATGGGTGGTAGTTGAAA+TGG 0.405959 1.4:+58671002 None:intergenic
AAGGAAAGAACTCTATTAAT+GGG 0.415197 1.4:+58670987 None:intergenic
TAGTCGCCCAAGAAAACTTC+AGG 0.419211 1.4:-58669165 MS.gene39959:CDS
GGCTCTCCTTTACCAGCTCC+AGG 0.442482 1.4:-58670778 MS.gene39959:intron
ATTGTGCATGGAATCCATTC+AGG 0.446420 1.4:+58670943 None:intergenic
CCATTCAGGTTGCATGATTG+TGG 0.485094 1.4:+58670957 None:intergenic
CATTAATTGGCGGAAGAGAC+GGG 0.492843 1.4:+58669439 None:intergenic
ATATTTACCTTGCGGACTCA+AGG 0.503866 1.4:+58669410 None:intergenic
ACATTTGATCCAAATATAGC+TGG 0.505446 1.4:+58669462 None:intergenic
CCACAATCATGCAACCTGAA+TGG 0.505888 1.4:-58670957 MS.gene39959:CDS
GCATTAATTGGCGGAAGAGA+CGG 0.508648 1.4:+58669438 None:intergenic
AGGAGAATCAGCTACCTCAA+TGG 0.512985 1.4:+58669250 None:intergenic
TGGATTCCATGCACAATGCA+AGG 0.527893 1.4:-58670937 MS.gene39959:CDS
TAAGTACCTGGAGCTGGTAA+AGG 0.529079 1.4:+58670772 None:intergenic
GCATGATTGTGGAAGGAAGA+AGG 0.544924 1.4:+58670968 None:intergenic
TAATAATAAGTACCTGGAGC+TGG 0.545527 1.4:+58670766 None:intergenic
TCAGGTTGCATGATTGTGGA+AGG 0.574464 1.4:+58670961 None:intergenic
TATTTACCTTGCGGACTCAA+GGG 0.591593 1.4:+58669411 None:intergenic
AAGGGAGAAGCATTAATTGG+CGG 0.596813 1.4:+58669429 None:intergenic
CGCAGTAGATGAAACCATTG+AGG 0.601013 1.4:-58669264 MS.gene39959:intron
GTGTACCCTTGCATTGTGCA+TGG 0.619403 1.4:+58670931 None:intergenic
GGATTCCATGCACAATGCAA+GGG 0.619466 1.4:-58670936 MS.gene39959:intron
TGCAATTCAACACTCAGCAA+AGG 0.626463 1.4:-58669222 MS.gene39959:CDS
GCTTCTCCCTTGAGTCCGCA+AGG 0.628732 1.4:-58669417 MS.gene39959:intron
GAAAGAACTCTATTAATGGG+TGG 0.644751 1.4:+58670990 None:intergenic
TGGTAGTTGAAATGGAACGT+TGG 0.743680 1.4:+58671010 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGAAACAATTTTTTTAA+AGG + chr1.4:58669992-58670011 None:intergenic 10.0%
!!! ATTTTAAAGGAAATTAAAAT+AGG + chr1.4:58669334-58669353 None:intergenic 10.0%
!!! TTATATACAAATGATTTTAA+AGG + chr1.4:58669347-58669366 None:intergenic 10.0%
!! ATGTAAACACAAAATTAAAA+GGG - chr1.4:58670830-58670849 MS.gene39959:intron 15.0%
!! GTATATAAAATGTCTAATAA+AGG - chr1.4:58669359-58669378 MS.gene39959:intron 15.0%
!! TATGTAAACACAAAATTAAA+AGG - chr1.4:58670829-58670848 MS.gene39959:intron 15.0%
!! TATGTAATTAAAAACTTGAT+CGG + chr1.4:58669619-58669638 None:intergenic 15.0%
!! TTTAATTAATAATAAGTACC+TGG + chr1.4:58669432-58669451 None:intergenic 15.0%
!!! AAATTATTTTACTTTCACTT+TGG - chr1.4:58669910-58669929 MS.gene39959:intron 15.0%
!!! ATGATCTTTAAAAATTATTC+AGG - chr1.4:58670656-58670675 MS.gene39959:intron 15.0%
!!! ATTTTTTATGTCAACAAATT+GGG + chr1.4:58670889-58670908 None:intergenic 15.0%
!!! TATTTTTTATGTCAACAAAT+TGG + chr1.4:58670890-58670909 None:intergenic 15.0%
!!! TTTTAAACATTTTATTTGCA+AGG - chr1.4:58669583-58669602 MS.gene39959:intron 15.0%
!! ATCACATAATTTATCACAAA+AGG + chr1.4:58669301-58669320 None:intergenic 20.0%
!! ATGTAACATTCATATATAAG+AGG + chr1.4:58670595-58670614 None:intergenic 20.0%
!! CAAATTAAATTTACTAGCTA+TGG - chr1.4:58670265-58670284 MS.gene39959:intron 20.0%
!! GTATCAAAATTAAACAAAAC+AGG + chr1.4:58669945-58669964 None:intergenic 20.0%
!!! TAGCTAGTAAATTTAATTTG+TGG + chr1.4:58670265-58670284 None:intergenic 20.0%
!!! TTGTGGTTATATTATACTTT+TGG + chr1.4:58670248-58670267 None:intergenic 20.0%
!!! TTTTGTGATAAATTATGTGA+TGG - chr1.4:58669300-58669319 MS.gene39959:intron 20.0%
! AAACACAAAATTAAAAGGGT+TGG - chr1.4:58670834-58670853 MS.gene39959:intron 25.0%
! AAGGAAAGAACTCTATTAAT+GGG + chr1.4:58669205-58669224 None:intergenic 25.0%
! ATTAGATACTTTGATGCTTT+AGG + chr1.4:58670082-58670101 None:intergenic 25.0%
! CAAACTGTAAAATGTTTGTA+AGG + chr1.4:58669967-58669986 None:intergenic 25.0%
! GATGTTCTCAAAAAAATTAG+TGG + chr1.4:58669704-58669723 None:intergenic 25.0%
! TAACAATGATATTTACCTTG+CGG + chr1.4:58670790-58670809 None:intergenic 25.0%
! TAATTAAAAACTTGATCGGA+TGG + chr1.4:58669615-58669634 None:intergenic 25.0%
!! CTTACAAACATTTTACAGTT+TGG - chr1.4:58669965-58669984 MS.gene39959:intron 25.0%
!! GTATCAAGTTTAAAACTACT+AGG + chr1.4:58670317-58670336 None:intergenic 25.0%
!! TGGAAATATATAAAGGTACA+TGG - chr1.4:58670631-58670650 MS.gene39959:intron 25.0%
!! TGGTCTCTTAAGTTAATTTT+AGG + chr1.4:58670228-58670247 None:intergenic 25.0%
!!! ATTGTTTTTAAGTAGTCCAA+TGG - chr1.4:58669460-58669479 MS.gene39959:CDS 25.0%
!!! CTCTCATTTGAGTTTTTAAA+CGG - chr1.4:58669735-58669754 MS.gene39959:intron 25.0%
!!! TGAGTTCTGGAAATATATAA+AGG - chr1.4:58670624-58670643 MS.gene39959:intron 25.0%
AAAAAGATGATAGCATGCTT+CGG + chr1.4:58670339-58670358 None:intergenic 30.0%
AAAGTGTGTCGAAAAAGAAT+AGG + chr1.4:58669659-58669678 None:intergenic 30.0%
AATTTGTATATTGCAGCTTC+TGG - chr1.4:58669390-58669409 MS.gene39959:intron 30.0%
ACAAATTGGGTGATGTATAA+TGG + chr1.4:58670876-58670895 None:intergenic 30.0%
ACATTTGATCCAAATATAGC+TGG + chr1.4:58670730-58670749 None:intergenic 30.0%
GAAAAAGAATAGGTTAGAGA+CGG + chr1.4:58669649-58669668 None:intergenic 30.0%
GAAGGAAAGAACTCTATTAA+TGG + chr1.4:58669206-58669225 None:intergenic 30.0%
TAAATAAGCACTCACATTTC+AGG + chr1.4:58670428-58670447 None:intergenic 30.0%
TCAACTGAACTTCATTTAGA+AGG - chr1.4:58669556-58669575 MS.gene39959:intron 30.0%
TGTTCAACGTTCATAACTTA+AGG + chr1.4:58670020-58670039 None:intergenic 30.0%
! TGAATTCCTGAAGTTTTCTT+GGG + chr1.4:58671033-58671052 None:intergenic 30.0%
! TTGAATTCCTGAAGTTTTCT+TGG + chr1.4:58671034-58671053 None:intergenic 30.0%
!! CAAAAAAATTAGTGGACTCA+TGG + chr1.4:58669696-58669715 None:intergenic 30.0%
!! GAGTGTTGAATTGCATATTT+AGG + chr1.4:58670962-58670981 None:intergenic 30.0%
!!! TTCATTGGTACTAGTTTTGT+CGG + chr1.4:58671001-58671020 None:intergenic 30.0%
CACAAATTCATGGTCAACAT+TGG + chr1.4:58670388-58670407 None:intergenic 35.0%
GAAAGAACTCTATTAATGGG+TGG + chr1.4:58669202-58669221 None:intergenic 35.0%
TAATAATAAGTACCTGGAGC+TGG + chr1.4:58669426-58669445 None:intergenic 35.0%
TTCATGGACATTAGGACATA+TGG + chr1.4:58669532-58669551 None:intergenic 35.0%
! ACATTAGGACATATGGATTG+AGG + chr1.4:58669525-58669544 None:intergenic 35.0%
! ATATCTGAGACCATCTATGA+TGG + chr1.4:58670492-58670511 None:intergenic 35.0%
! ATTTTCTATGGCATAAGCCA+TGG + chr1.4:58670365-58670384 None:intergenic 35.0%
! GCTATCATCTTTTTATGCCA+TGG - chr1.4:58670345-58670364 MS.gene39959:intron 35.0%
! GGTCAACATTGGATTTTCTA+TGG + chr1.4:58670377-58670396 None:intergenic 35.0%
! TTAATGGGTGGTAGTTGAAA+TGG + chr1.4:58669190-58669209 None:intergenic 35.0%
! TTGATAGCTTCATGGACATT+AGG + chr1.4:58669540-58669559 None:intergenic 35.0%
!! AAGTTCAGTTGATAGCTTCA+TGG + chr1.4:58669548-58669567 None:intergenic 35.0%
ATAAGCACTCACATTTCAGG+AGG + chr1.4:58670425-58670444 None:intergenic 40.0%
ATATTTACCTTGCGGACTCA+AGG + chr1.4:58670782-58670801 None:intergenic 40.0%
ATTGTGCATGGAATCCATTC+AGG + chr1.4:58669249-58669268 None:intergenic 40.0%
CACACTGTCTCACAAATTCA+TGG + chr1.4:58670398-58670417 None:intergenic 40.0%
CCAACAAAAGCAAAGGAAAG+AGG + chr1.4:58670702-58670721 None:intergenic 40.0%
TAAGCACTCACATTTCAGGA+GGG + chr1.4:58670424-58670443 None:intergenic 40.0%
TATTTACCTTGCGGACTCAA+GGG + chr1.4:58670781-58670800 None:intergenic 40.0%
TGCAATTCAACACTCAGCAA+AGG - chr1.4:58670967-58670986 MS.gene39959:CDS 40.0%
TGGTAGTTGAAATGGAACGT+TGG + chr1.4:58669182-58669201 None:intergenic 40.0%
!! AAGGGAGAAGCATTAATTGG+CGG + chr1.4:58670763-58670782 None:intergenic 40.0%
!! CCTCTTTCCTTTGCTTTTGT+TGG - chr1.4:58670699-58670718 MS.gene39959:intron 40.0%
!! CTCAAGGGAGAAGCATTAAT+TGG + chr1.4:58670766-58670785 None:intergenic 40.0%
!! TTTCCTTTGCTTTTGTTGGC+AGG - chr1.4:58670703-58670722 MS.gene39959:intron 40.0%
AAGCACTCACATTTCAGGAG+GGG + chr1.4:58670423-58670442 None:intergenic 45.0%
ACATGCTCAACGTTGAGTTC+TGG - chr1.4:58670611-58670630 MS.gene39959:intron 45.0%
AGGAGAATCAGCTACCTCAA+TGG + chr1.4:58670942-58670961 None:intergenic 45.0%
CATTAATTGGCGGAAGAGAC+GGG + chr1.4:58670753-58670772 None:intergenic 45.0%
CCACAATCATGCAACCTGAA+TGG - chr1.4:58669232-58669251 MS.gene39959:CDS 45.0%
CGCAGTAGATGAAACCATTG+AGG - chr1.4:58670925-58670944 MS.gene39959:intron 45.0%
CTCCACATGAGTTTAGCCAT+TGG + chr1.4:58669479-58669498 None:intergenic 45.0%
GCATGATTGTGGAAGGAAGA+AGG + chr1.4:58669224-58669243 None:intergenic 45.0%
GCATTAATTGGCGGAAGAGA+CGG + chr1.4:58670754-58670773 None:intergenic 45.0%
GGATTCCATGCACAATGCAA+GGG - chr1.4:58669253-58669272 MS.gene39959:CDS 45.0%
GTCCAATGGCTAAACTCATG+TGG - chr1.4:58669474-58669493 MS.gene39959:intron 45.0%
TAAGTACCTGGAGCTGGTAA+AGG + chr1.4:58669420-58669439 None:intergenic 45.0%
TAGTCGCCCAAGAAAACTTC+AGG - chr1.4:58671024-58671043 MS.gene39959:CDS 45.0%
TGGATTCCATGCACAATGCA+AGG - chr1.4:58669252-58669271 MS.gene39959:CDS 45.0%
TTGGCAGGTCCAGCTATATT+TGG - chr1.4:58670718-58670737 MS.gene39959:intron 45.0%
TTTCTCCACGCCATCATAGA+TGG - chr1.4:58670479-58670498 MS.gene39959:intron 45.0%
! TCAGGTTGCATGATTGTGGA+AGG + chr1.4:58669231-58669250 None:intergenic 45.0%
! TCTATGATGGCGTGGAGAAA+AGG + chr1.4:58670479-58670498 None:intergenic 45.0%
!! CCATTCAGGTTGCATGATTG+TGG + chr1.4:58669235-58669254 None:intergenic 45.0%
CTTGGGCGACTATGCTTCAT+TGG + chr1.4:58671016-58671035 None:intergenic 50.0%
GGACCTGCCAACAAAAGCAA+AGG + chr1.4:58670709-58670728 None:intergenic 50.0%
GTGTACCCTTGCATTGTGCA+TGG + chr1.4:58669261-58669280 None:intergenic 50.0%
! TGAGACCATCTATGATGGCG+TGG + chr1.4:58670487-58670506 None:intergenic 50.0%
!!! GGTACTAGTTTTGTCGGTGC+TGG + chr1.4:58670995-58671014 None:intergenic 50.0%
GCTTCTCCCTTGAGTCCGCA+AGG - chr1.4:58670772-58670791 MS.gene39959:intron 60.0%
GGCTCTCCTTTACCAGCTCC+AGG - chr1.4:58669411-58669430 MS.gene39959:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 58669151 58671060 58669151 ID=MS.gene39959
chr1.4 mRNA 58669151 58671060 58669151 ID=MS.gene39959.t1;Parent=MS.gene39959
chr1.4 exon 58670937 58671060 58670937 ID=MS.gene39959.t1.exon1;Parent=MS.gene39959.t1
chr1.4 CDS 58670937 58671060 58670937 ID=cds.MS.gene39959.t1;Parent=MS.gene39959.t1
chr1.4 exon 58670779 58670805 58670779 ID=MS.gene39959.t1.exon2;Parent=MS.gene39959.t1
chr1.4 CDS 58670779 58670805 58670779 ID=cds.MS.gene39959.t1;Parent=MS.gene39959.t1
chr1.4 exon 58669418 58669486 58669418 ID=MS.gene39959.t1.exon3;Parent=MS.gene39959.t1
chr1.4 CDS 58669418 58669486 58669418 ID=cds.MS.gene39959.t1;Parent=MS.gene39959.t1
chr1.4 exon 58669151 58669281 58669151 ID=MS.gene39959.t1.exon4;Parent=MS.gene39959.t1
chr1.4 CDS 58669151 58669281 58669151 ID=cds.MS.gene39959.t1;Parent=MS.gene39959.t1
Gene Sequence

>MS.gene39959

ATGGGTTGTGCTTGCTTGTTAATTACAGCCAACGTTCCATTTCAACTACCACCCATTAATAGAGTTCTTTCCTTCTTCCTTCCACAATCATGCAACCTGAATGGATTCCATGCACAATGCAAGGGTACACAATTCATATATATGTTTCCTTTTGTGATAAATTATGTGATGGATTTTCTTCCTATTTTAATTTCCTTTAAAATCATTTGTATATAAAATGTCTAATAAAGGTTTTTATAAATTTGTATATTGCAGCTTCTGGCTCTCCTTTACCAGCTCCAGGTACTTATTATTAATTAAATTAACTCAATTGTTTTTAAGTAGTCCAATGGCTAAACTCATGTGGAGAAGTGAGAAATTTGACGTTCGAACCTCAATCCATATGTCCTAATGTCCATGAAGCTATCAACTGAACTTCATTTAGAAGGTTGCTTTTAAACATTTTATTTGCAAGGACAGTACCATCCGATCAAGTTTTTAATTACATAAGTAACACCGTCTCTAACCTATTCTTTTTCGACACACTTTTTATAGATTAAACTCCATGAGTCCACTAATTTTTTTGAGAACATCACAATGAAGCTCTCTCATTTGAGTTTTTAAACGGATCTTGTATTCTTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATGTCACTTATATTATAGTTTTTTTTAATGTTGATACTTAATAAACACTCAACTTCTCAAACTCTTAAAATTATTTTACTTTCACTTTGGTCTCTTAAGCCTGTTTTGTTTAATTTTGATACCTTACAAACATTTTACAGTTTGGTCCTTTAAAAAAATTGTTTCATTTTGCTACCTTAAGTTATGAACGTTGAACACTTTGATCTCGAAAAGACTAAAGTGTCAAAAGTTTATAACCTAAAGCATCAAAGTATCTAATATTTATAAGTTAAGTGATCAAAATGAAATGAAAAAAAAAAAAACTTCGTGATCAAATTGTCTAACATTTGTAATTTATAAAAACAAAGTGAAAAGAAAAAAAAAACTTAAAATCAAAGTGAAACCTAAAATTAACTTAAGAGACCAAAAGTATAATATAACCACAAATTAAATTTACTAGCTATGGAAAATGTTGTATCTCGCAAGAAAATTCCTAGTAGTTTTAAACTTGATACCGAAGCATGCTATCATCTTTTTATGCCATGGCTTATGCCATAGAAAATCCAATGTTGACCATGAATTTGTGAGACAGTGTGAACCCCTCCTGAAATGTGAGTGCTTATTTAAACTATTCAATTCAATAAACATCTTCAACCTTTTCTCCACGCCATCATAGATGGTCTCAGATATAAATAATTAATAATACTCGTTTGAATTTCAATCAATAGTATATATATCATAAAGAGTGTGTTACACATTACATTATTAATCCTCTTATATATGAATGTTACATGCTCAACGTTGAGTTCTGGAAATATATAAAGGTACATGGATATGATCTTTAAAAATTATTCAGGATCATGTCATAAATCTTATTCCTCTTTCCTTTGCTTTTGTTGGCAGGTCCAGCTATATTTGGATCAAATGTCCCGTCTCTTCCGCCAATTAATGCTTCTCCCTTGAGTCCGCAAGGTAAATATCATTGTTATTGTAAAAATTATATTTATTATGTAAACACAAAATTAAAAGGGTTGGTGACATTTATTTTCTTCCATTATACATCACCCAATTTGTTGACATAAAAAATATTGTCTAAACTTCAACGCAGTAGATGAAACCATTGAGGTAGCTGATTCTCCTAAATATGCAATTCAACACTCAGCAAAGGCACCAGCACCGACAAAACTAGTACCAATGAAGCATAGTCGCCCAAGAAAACTTCAGGAATTCAACAAGTAG

Protein sequence

>MS.gene39959.t1

MGCACLLITANVPFQLPPINRVLSFFLPQSCNLNGFHAQCKASGSPLPAPGPAIFGSNVPSLPPINASPLSPQVDETIEVADSPKYAIQHSAKAPAPTKLVPMKHSRPRKLQEFNK