AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56510


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56510.t1 MTR_4g082350 98.883 358 4 0 1 358 1 358 0.0 723
MS.gene56510.t1 MTR_4g109020 28.082 292 198 9 49 331 6 294 7.34e-29 114
MS.gene56510.t1 MTR_3g037110 27.703 296 186 8 49 330 6 287 6.45e-26 106
MS.gene56510.t1 MTR_4g096890 26.073 303 197 7 41 336 7 289 1.80e-17 82.4
MS.gene56510.t1 MTR_7g021620 26.316 304 199 7 39 336 5 289 8.51e-17 80.5
MS.gene56510.t1 MTR_3g088975 24.756 307 211 6 39 336 5 300 2.06e-15 76.6
MS.gene56510.t1 MTR_1g029440 23.127 307 225 6 35 336 1 301 1.05e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56510.t1 AT1G69430 66.462 325 102 2 17 335 8 331 2.83e-154 437
MS.gene56510.t1 AT1G26650 63.939 330 116 3 24 350 2 331 8.05e-154 436
MS.gene56510.t1 AT4G19950 27.451 306 190 9 41 336 7 290 8.72e-15 74.7
MS.gene56510.t1 AT5G61340 28.311 219 136 8 119 329 80 285 1.57e-12 68.2
MS.gene56510.t1 AT5G61340 28.311 219 136 8 119 329 113 318 1.59e-12 68.6

Find 67 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGGTTCTGGAGGATGTTTC+GGG 0.171105 4.2:+27373738 MS.gene56510:CDS
CTCGGTTCTGGAGGATGTTT+CGG 0.203669 4.2:+27373737 MS.gene56510:CDS
GAGAAACTATAAGGATTCTT+AGG 0.245365 4.2:+27373169 MS.gene56510:CDS
TGTAGTGCATTGGCTGTTCT+TGG 0.255096 4.2:+27373612 MS.gene56510:CDS
TTGTGAAGAATCTTACTATT+AGG 0.290531 4.2:+27373283 MS.gene56510:CDS
GAGGTTGTTCTCCTAGGATT+AGG 0.293873 4.2:+27373031 MS.gene56510:CDS
TGCGTTGGTGATCTCGGTTC+TGG 0.297916 4.2:+27373725 MS.gene56510:CDS
TATTCGAGAAAGAAGTTTGA+TGG 0.334246 4.2:+27373471 MS.gene56510:CDS
TTTACGTCAAGCTTTAGAAT+TGG 0.356836 4.2:+27373092 MS.gene56510:CDS
ATATTCTTAGAGAAACTATA+AGG 0.357854 4.2:+27373160 MS.gene56510:CDS
GCCCATTGTTTCAGCAGAAA+TGG 0.362209 4.2:-27374060 None:intergenic
AATTCAATCAATGAATGCTT+TGG 0.379030 4.2:+27373137 MS.gene56510:CDS
TGTGCTATTGTCTAATGTAT+TGG 0.379670 4.2:+27373248 MS.gene56510:CDS
ATATATCTTGGATCAACAAT+AGG 0.400163 4.2:+27373822 MS.gene56510:CDS
TGTTGTGTATGCTGCATTGA+TGG 0.401523 4.2:+27373650 MS.gene56510:CDS
AGGTTGTTCTCCTAGGATTA+GGG 0.404867 4.2:+27373032 MS.gene56510:CDS
CAGCGTTGCGTTGGTGATCT+CGG 0.412590 4.2:+27373719 MS.gene56510:CDS
GTGTGATCGTGGCAAAGTTT+TGG 0.412812 4.2:+27373505 MS.gene56510:CDS
GTCGGTCTGCTAATATATCT+TGG 0.415716 4.2:+27373810 MS.gene56510:CDS
GCAGCATACACAACAACATT+AGG 0.417117 4.2:-27373642 None:intergenic
GGCCATTTCTGCTGAAACAA+TGG 0.429412 4.2:+27374058 MS.gene56510:CDS
GACAATAGCACAGCAGAAAC+AGG 0.430186 4.2:-27373237 None:intergenic
AACAATAGGAATGGCCTTTG+TGG 0.433278 4.2:+27373836 MS.gene56510:CDS
TGCGGATACGGTGATTGCTT+CGG 0.443552 4.2:+27373374 MS.gene56510:CDS
TGGGCTTGTACAGTTATTGT+TGG 0.445242 4.2:+27373552 MS.gene56510:CDS
ATTGTTGTTATTGTCGAAAA+CGG 0.446504 4.2:+27373422 MS.gene56510:CDS
CAAGTGGAGGTTGTTCTCCT+AGG 0.449328 4.2:+27373025 MS.gene56510:CDS
GTAGTGCATTGGCTGTTCTT+GGG 0.473904 4.2:+27373613 MS.gene56510:CDS
GCCATTTCTGCTGAAACAAT+GGG 0.478130 4.2:+27374059 MS.gene56510:CDS
AGATACTTGCGACGTATATG+TGG 0.480298 4.2:+27373532 MS.gene56510:CDS
TTGTCAGAGATTTGCGGATA+CGG 0.482242 4.2:+27373362 MS.gene56510:CDS
TGACAAGATTGCTTAATCAT+AGG 0.488189 4.2:-27373345 None:intergenic
AATCATTTGCAGCGTTGCGT+TGG 0.493610 4.2:+27373710 MS.gene56510:CDS
GGATTCTTAGGTTTAATGCA+TGG 0.498954 4.2:+27373181 MS.gene56510:CDS
TCTTACTATTAGGCTTATGT+TGG 0.501948 4.2:+27373293 MS.gene56510:CDS
TTTAGCATGGAGAACTCAAA+TGG 0.507639 4.2:+27374008 MS.gene56510:CDS
GCAATCTTGTCAGAGATTTG+CGG 0.510716 4.2:+27373356 MS.gene56510:CDS
ATATGATCTACCCTAATCCT+AGG 0.527505 4.2:-27373042 None:intergenic
AATGTGGGAAGGGCCGCTCT+TGG 0.532814 4.2:+27373914 MS.gene56510:CDS
GATTCTTAGGTTTAATGCAT+GGG 0.536767 4.2:+27373182 MS.gene56510:CDS
ATAGGAATGGCCTTTGTGGA+GGG 0.546492 4.2:+27373840 MS.gene56510:CDS
TGCTTAATCATAGGTCTCAA+AGG 0.555452 4.2:-27373336 None:intergenic
GTTATTGTCGAAAACGGCTG+TGG 0.558964 4.2:+27373428 MS.gene56510:CDS
GTGTATGCTGCATTGATGGT+CGG 0.566529 4.2:+27373654 MS.gene56510:CDS
GTGATGGATCTTCGAGAATG+TGG 0.574992 4.2:+27373898 MS.gene56510:CDS
AATAGGAATGGCCTTTGTGG+AGG 0.586579 4.2:+27373839 MS.gene56510:CDS
TCTTGGATCAACAATAGGAA+TGG 0.587680 4.2:+27373827 MS.gene56510:CDS
TTTAATCAAAGGTCAGACTC+AGG 0.592291 4.2:+27373788 MS.gene56510:CDS
AGAGTAAAGACACTGAGTTA+TGG 0.598968 4.2:+27373876 MS.gene56510:CDS
TAGGAATGGCCTTTGTGGAG+GGG 0.600285 4.2:+27373841 MS.gene56510:CDS
ATCAAAGGTCAGACTCAGGT+CGG 0.608137 4.2:+27373792 MS.gene56510:CDS
CGGTTCTGGAGGATGTTTCG+GGG 0.610462 4.2:+27373739 MS.gene56510:CDS
AAAATGGAGATTTCAAGTGG+AGG 0.612604 4.2:+27373012 None:intergenic
ATGGAGAACTCAAATGGCGA+TGG 0.614239 4.2:+27374014 MS.gene56510:CDS
ATGTTGGTTGCTGAAACTAG+TGG 0.620640 4.2:+27373309 MS.gene56510:CDS
TATAGATTCTATGATGAGTG+CGG 0.621002 4.2:+27373962 MS.gene56510:CDS
ATGCACTACAAAACGCAACA+AGG 0.631744 4.2:-27373599 None:intergenic
GATACTTGCGACGTATATGT+GGG 0.634555 4.2:+27373533 MS.gene56510:CDS
AACAACAATGTAGCATACAA+AGG 0.636060 4.2:-27373408 None:intergenic
GTTGGTGATCTCGGTTCTGG+AGG 0.638193 4.2:+27373728 MS.gene56510:CDS
AAGACACTGAGTTATGGTGA+TGG 0.640856 4.2:+27373882 MS.gene56510:CDS
GATCTTCGAGAATGTGGGAA+GGG 0.641099 4.2:+27373904 MS.gene56510:CDS
GGATCTTCGAGAATGTGGGA+AGG 0.642671 4.2:+27373903 MS.gene56510:CDS
CTCAAACAACCCCTCCACAA+AGG 0.646189 4.2:-27373850 None:intergenic
GAATACATTATCACCAAGAG+CGG 0.651587 4.2:-27373927 None:intergenic
GGTTCTGGAGGATGTTTCGG+GGG 0.680880 4.2:+27373740 MS.gene56510:CDS
TGATGGATCTTCGAGAATGT+GGG 0.681312 4.2:+27373899 MS.gene56510:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTCTTAGAGAAACTATA+AGG + chr4.2:27373160-27373179 MS.gene56510:CDS 20.0%
! AATTCAATCAATGAATGCTT+TGG + chr4.2:27373137-27373156 MS.gene56510:CDS 25.0%
! ATATATCTTGGATCAACAAT+AGG + chr4.2:27373822-27373841 MS.gene56510:CDS 25.0%
! GTAGATCATATGAAAAAAGA+GGG + chr4.2:27373054-27373073 MS.gene56510:CDS 25.0%
! TTGTGAAGAATCTTACTATT+AGG + chr4.2:27373283-27373302 MS.gene56510:CDS 25.0%
!! ATTGTTGTTATTGTCGAAAA+CGG + chr4.2:27373422-27373441 MS.gene56510:CDS 25.0%
!!! CAACTCAATTTTAGAAACTA+TGG + chr4.2:27374037-27374056 MS.gene56510:CDS 25.0%
AACAACAATGTAGCATACAA+AGG - chr4.2:27373411-27373430 None:intergenic 30.0%
GAGAAACTATAAGGATTCTT+AGG + chr4.2:27373169-27373188 MS.gene56510:CDS 30.0%
GATTCTTAGGTTTAATGCAT+GGG + chr4.2:27373182-27373201 MS.gene56510:CDS 30.0%
GGTAGATCATATGAAAAAAG+AGG + chr4.2:27373053-27373072 MS.gene56510:CDS 30.0%
TATAGATTCTATGATGAGTG+CGG + chr4.2:27373962-27373981 MS.gene56510:CDS 30.0%
TATTCGAGAAAGAAGTTTGA+TGG + chr4.2:27373471-27373490 MS.gene56510:CDS 30.0%
TCTTACTATTAGGCTTATGT+TGG + chr4.2:27373293-27373312 MS.gene56510:CDS 30.0%
TGACAAGATTGCTTAATCAT+AGG - chr4.2:27373348-27373367 None:intergenic 30.0%
! TGTGCTATTGTCTAATGTAT+TGG + chr4.2:27373248-27373267 MS.gene56510:CDS 30.0%
!! GGTGATAATGTATTCTTTTG+TGG + chr4.2:27373935-27373954 MS.gene56510:CDS 30.0%
!! TTTACGTCAAGCTTTAGAAT+TGG + chr4.2:27373092-27373111 MS.gene56510:CDS 30.0%
!!! AGGTCGAGTATTTTAATCAA+AGG + chr4.2:27373777-27373796 MS.gene56510:CDS 30.0%
AGAGTAAAGACACTGAGTTA+TGG + chr4.2:27373876-27373895 MS.gene56510:CDS 35.0%
ATATGATCTACCCTAATCCT+AGG - chr4.2:27373045-27373064 None:intergenic 35.0%
GAATACATTATCACCAAGAG+CGG - chr4.2:27373930-27373949 None:intergenic 35.0%
GGATTCTTAGGTTTAATGCA+TGG + chr4.2:27373181-27373200 MS.gene56510:CDS 35.0%
TCTTGGATCAACAATAGGAA+TGG + chr4.2:27373827-27373846 MS.gene56510:CDS 35.0%
TGCTTAATCATAGGTCTCAA+AGG - chr4.2:27373339-27373358 None:intergenic 35.0%
TTTAATCAAAGGTCAGACTC+AGG + chr4.2:27373788-27373807 MS.gene56510:CDS 35.0%
TTTAGCATGGAGAACTCAAA+TGG + chr4.2:27374008-27374027 MS.gene56510:CDS 35.0%
! CAGTTGTAGATCTTTTAGCA+TGG + chr4.2:27373995-27374014 MS.gene56510:CDS 35.0%
AACAATAGGAATGGCCTTTG+TGG + chr4.2:27373836-27373855 MS.gene56510:CDS 40.0%
AAGACACTGAGTTATGGTGA+TGG + chr4.2:27373882-27373901 MS.gene56510:CDS 40.0%
AGATACTTGCGACGTATATG+TGG + chr4.2:27373532-27373551 MS.gene56510:CDS 40.0%
AGGTTGTTCTCCTAGGATTA+GGG + chr4.2:27373032-27373051 MS.gene56510:CDS 40.0%
ATGCACTACAAAACGCAACA+AGG - chr4.2:27373602-27373621 None:intergenic 40.0%
GATACTTGCGACGTATATGT+GGG + chr4.2:27373533-27373552 MS.gene56510:CDS 40.0%
GCAATCTTGTCAGAGATTTG+CGG + chr4.2:27373356-27373375 MS.gene56510:CDS 40.0%
GCAGCATACACAACAACATT+AGG - chr4.2:27373645-27373664 None:intergenic 40.0%
GCCATTTCTGCTGAAACAAT+GGG + chr4.2:27374059-27374078 MS.gene56510:CDS 40.0%
GTCGGTCTGCTAATATATCT+TGG + chr4.2:27373810-27373829 MS.gene56510:CDS 40.0%
TGGGCTTGTACAGTTATTGT+TGG + chr4.2:27373552-27373571 MS.gene56510:CDS 40.0%
TGTTGTGTATGCTGCATTGA+TGG + chr4.2:27373650-27373669 MS.gene56510:CDS 40.0%
TTGTCAGAGATTTGCGGATA+CGG + chr4.2:27373362-27373381 MS.gene56510:CDS 40.0%
! ATGTTGGTTGCTGAAACTAG+TGG + chr4.2:27373309-27373328 MS.gene56510:CDS 40.0%
! TGATGGATCTTCGAGAATGT+GGG + chr4.2:27373899-27373918 MS.gene56510:CDS 40.0%
! TGTTGCGTTTTGTAGTGCAT+TGG + chr4.2:27373602-27373621 MS.gene56510:CDS 40.0%
!! TTCGAAGTTTTGTGTGATCG+TGG + chr4.2:27373494-27373513 MS.gene56510:CDS 40.0%
AATAGGAATGGCCTTTGTGG+AGG + chr4.2:27373839-27373858 MS.gene56510:CDS 45.0%
AATCATTTGCAGCGTTGCGT+TGG + chr4.2:27373710-27373729 MS.gene56510:CDS 45.0%
ATAGGAATGGCCTTTGTGGA+GGG + chr4.2:27373840-27373859 MS.gene56510:CDS 45.0%
ATCAAAGGTCAGACTCAGGT+CGG + chr4.2:27373792-27373811 MS.gene56510:CDS 45.0%
ATGGAGAACTCAAATGGCGA+TGG + chr4.2:27374014-27374033 MS.gene56510:CDS 45.0%
GACAATAGCACAGCAGAAAC+AGG - chr4.2:27373240-27373259 None:intergenic 45.0%
GAGGTTGTTCTCCTAGGATT+AGG + chr4.2:27373031-27373050 MS.gene56510:CDS 45.0%
GATCTTCGAGAATGTGGGAA+GGG + chr4.2:27373904-27373923 MS.gene56510:CDS 45.0%
GCCCATTGTTTCAGCAGAAA+TGG - chr4.2:27374063-27374082 None:intergenic 45.0%
GGCCATTTCTGCTGAAACAA+TGG + chr4.2:27374058-27374077 MS.gene56510:CDS 45.0%
GTTATTGTCGAAAACGGCTG+TGG + chr4.2:27373428-27373447 MS.gene56510:CDS 45.0%
! GTGTATGCTGCATTGATGGT+CGG + chr4.2:27373654-27373673 MS.gene56510:CDS 45.0%
! GTGTGATCGTGGCAAAGTTT+TGG + chr4.2:27373505-27373524 MS.gene56510:CDS 45.0%
!! GTAGTGCATTGGCTGTTCTT+GGG + chr4.2:27373613-27373632 MS.gene56510:CDS 45.0%
!! GTGATGGATCTTCGAGAATG+TGG + chr4.2:27373898-27373917 MS.gene56510:CDS 45.0%
!! TGATCGTGGCAAAGTTTTGG+AGG + chr4.2:27373508-27373527 MS.gene56510:CDS 45.0%
!! TGTAGTGCATTGGCTGTTCT+TGG + chr4.2:27373612-27373631 MS.gene56510:CDS 45.0%
CAAGTGGAGGTTGTTCTCCT+AGG + chr4.2:27373025-27373044 MS.gene56510:CDS 50.0%
CTCAAACAACCCCTCCACAA+AGG - chr4.2:27373853-27373872 None:intergenic 50.0%
GGATCTTCGAGAATGTGGGA+AGG + chr4.2:27373903-27373922 MS.gene56510:CDS 50.0%
TAGGAATGGCCTTTGTGGAG+GGG + chr4.2:27373841-27373860 MS.gene56510:CDS 50.0%
TGCGGATACGGTGATTGCTT+CGG + chr4.2:27373374-27373393 MS.gene56510:CDS 50.0%
!! CTCGGTTCTGGAGGATGTTT+CGG + chr4.2:27373737-27373756 MS.gene56510:CDS 50.0%
!! TCGGTTCTGGAGGATGTTTC+GGG + chr4.2:27373738-27373757 MS.gene56510:CDS 50.0%
!! CAGCGTTGCGTTGGTGATCT+CGG + chr4.2:27373719-27373738 MS.gene56510:CDS 55.0%
!! CGGTTCTGGAGGATGTTTCG+GGG + chr4.2:27373739-27373758 MS.gene56510:CDS 55.0%
!! GGTTCTGGAGGATGTTTCGG+GGG + chr4.2:27373740-27373759 MS.gene56510:CDS 55.0%
!! GTTGGTGATCTCGGTTCTGG+AGG + chr4.2:27373728-27373747 MS.gene56510:CDS 55.0%
!! TGCGTTGGTGATCTCGGTTC+TGG + chr4.2:27373725-27373744 MS.gene56510:CDS 55.0%
AATGTGGGAAGGGCCGCTCT+TGG + chr4.2:27373914-27373933 MS.gene56510:CDS 60.0%
! GGAGGATGTTTCGGGGGCGC+AGG + chr4.2:27373746-27373765 MS.gene56510:CDS 70.0%
! CGGGGGCGCAGGCGATGCTA+AGG + chr4.2:27373757-27373776 MS.gene56510:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 27373015 27374091 27373015 ID=MS.gene56510
chr4.2 mRNA 27373015 27374091 27373015 ID=MS.gene56510.t1;Parent=MS.gene56510
chr4.2 exon 27373015 27374091 27373015 ID=MS.gene56510.t1.exon1;Parent=MS.gene56510.t1
chr4.2 CDS 27373015 27374091 27373015 ID=cds.MS.gene56510.t1;Parent=MS.gene56510.t1
Gene Sequence

>MS.gene56510

ATGGAGATTTCAAGTGGAGGTTGTTCTCCTAGGATTAGGGTAGATCATATGAAAAAAGAGGGATCAGATGATACACGTTTACGTCAAGCTTTAGAATTGGAACTTTCTGATCATAATCATGAAATTCAATCAATGAATGCTTTGGATATTCTTAGAGAAACTATAAGGATTCTTAGGTTTAATGCATGGGCTTTTATGATAATCGCTTTTTTGCTTATTTGTCCTGTTTCTGCTGTGCTATTGTCTAATGTATTGGTTGATGAATCTATTGTGAAGAATCTTACTATTAGGCTTATGTTGGTTGCTGAAACTAGTGGTCTTCCTTTGAGACCTATGATTAAGCAATCTTGTCAGAGATTTGCGGATACGGTGATTGCTTCGGCGATGTGTTTTCCTTTGTATGCTACATTGTTGTTATTGTCGAAAACGGCTGTGGTTTATTCTGTTGATTGTACTTATTCGAGAAAGAAGTTTGATGGTTCGAAGTTTTGTGTGATCGTGGCAAAGTTTTGGAGGAAGATACTTGCGACGTATATGTGGGCTTGTACAGTTATTGTTGGTTGCATCACTATGTTTTGTGTTTTCCTTGTTGCGTTTTGTAGTGCATTGGCTGTTCTTGGGTTTTCTCCTAATGTTGTTGTGTATGCTGCATTGATGGTCGGATTAGTTTTCTCTGTTGTATTTGCGAATGCGATAATCATTTGCAGCGTTGCGTTGGTGATCTCGGTTCTGGAGGATGTTTCGGGGGCGCAGGCGATGCTAAGGTCGAGTATTTTAATCAAAGGTCAGACTCAGGTCGGTCTGCTAATATATCTTGGATCAACAATAGGAATGGCCTTTGTGGAGGGGTTGTTTGAGCACAGAGTAAAGACACTGAGTTATGGTGATGGATCTTCGAGAATGTGGGAAGGGCCGCTCTTGGTGATAATGTATTCTTTTGTGGTGCTTATAGATTCTATGATGAGTGCGGTTTTCTATTTCAGTTGTAGATCTTTTAGCATGGAGAACTCAAATGGCGATGGCAACTCAATTTTAGAAACTATGGCCATTTCTGCTGAAACAATGGGCATTCAATGA

Protein sequence

>MS.gene56510.t1

MEISSGGCSPRIRVDHMKKEGSDDTRLRQALELELSDHNHEIQSMNALDILRETIRILRFNAWAFMIIAFLLICPVSAVLLSNVLVDESIVKNLTIRLMLVAETSGLPLRPMIKQSCQRFADTVIASAMCFPLYATLLLLSKTAVVYSVDCTYSRKKFDGSKFCVIVAKFWRKILATYMWACTVIVGCITMFCVFLVAFCSALAVLGFSPNVVVYAALMVGLVFSVVFANAIIICSVALVISVLEDVSGAQAMLRSSILIKGQTQVGLLIYLGSTIGMAFVEGLFEHRVKTLSYGDGSSRMWEGPLLVIMYSFVVLIDSMMSAVFYFSCRSFSMENSNGDGNSILETMAISAETMGIQ