Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75719.t1 | XP_003601603.1 | 95.8 | 72 | 2 | 1 | 1 | 71 | 154 | 225 | 4.70E-30 | 141.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75719.t1 | Q9FYK2 | 63.4 | 71 | 25 | 1 | 1 | 70 | 108 | 178 | 1.4e-19 | 97.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75719.t1 | G7J9L5 | 95.8 | 72 | 2 | 1 | 1 | 71 | 154 | 225 | 3.4e-30 | 141.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene75719 | 0.811066 | 8.43E-51 | -1.69E-46 |
| MS.gene049350 | MS.gene75719 | 0.834796 | 2.44E-56 | -1.69E-46 |
| MS.gene049368 | MS.gene75719 | 0.804062 | 2.59E-49 | -1.69E-46 |
| MS.gene049499 | MS.gene75719 | 0.802998 | 4.30E-49 | -1.69E-46 |
| MS.gene049711 | MS.gene75719 | 0.828778 | 7.44E-55 | -1.69E-46 |
| MS.gene049807 | MS.gene75719 | 0.841677 | 4.11E-58 | -1.69E-46 |
| MS.gene049983 | MS.gene75719 | 0.800621 | 1.32E-48 | -1.69E-46 |
| MS.gene050253 | MS.gene75719 | 0.801092 | 1.06E-48 | -1.69E-46 |
| MS.gene050300 | MS.gene75719 | -0.818524 | 1.87E-52 | -1.69E-46 |
| MS.gene050414 | MS.gene75719 | 0.821628 | 3.65E-53 | -1.69E-46 |
| MS.gene051700 | MS.gene75719 | 0.801096 | 1.06E-48 | -1.69E-46 |
| MS.gene051719 | MS.gene75719 | 0.811883 | 5.60E-51 | -1.69E-46 |
| MS.gene051720 | MS.gene75719 | 0.821124 | 4.77E-53 | -1.69E-46 |
| MS.gene051852 | MS.gene75719 | 0.837267 | 5.75E-57 | -1.69E-46 |
| MS.gene052016 | MS.gene75719 | 0.823426 | 1.39E-53 | -1.69E-46 |
| MS.gene052307 | MS.gene75719 | 0.825264 | 5.15E-54 | -1.69E-46 |
| MS.gene052335 | MS.gene75719 | 0.814807 | 1.28E-51 | -1.69E-46 |
| MS.gene052342 | MS.gene75719 | 0.818117 | 2.32E-52 | -1.69E-46 |
| MS.gene052463 | MS.gene75719 | 0.859979 | 2.84E-63 | -1.69E-46 |
| MS.gene052516 | MS.gene75719 | 0.816328 | 5.85E-52 | -1.69E-46 |
| MS.gene052673 | MS.gene75719 | -0.829455 | 5.10E-55 | -1.69E-46 |
| MS.gene053079 | MS.gene75719 | 0.803201 | 3.90E-49 | -1.69E-46 |
| MS.gene053144 | MS.gene75719 | 0.806135 | 9.53E-50 | -1.69E-46 |
| MS.gene053164 | MS.gene75719 | 0.807723 | 4.40E-50 | -1.69E-46 |
| MS.gene053581 | MS.gene75719 | 0.836095 | 1.14E-56 | -1.69E-46 |
| MS.gene054178 | MS.gene75719 | 0.807781 | 4.27E-50 | -1.69E-46 |
| MS.gene05423 | MS.gene75719 | 0.813393 | 2.62E-51 | -1.69E-46 |
| MS.gene054584 | MS.gene75719 | 0.800841 | 1.19E-48 | -1.69E-46 |
| MS.gene05458 | MS.gene75719 | 0.814976 | 1.17E-51 | -1.69E-46 |
| MS.gene054772 | MS.gene75719 | 0.814454 | 1.53E-51 | -1.69E-46 |
| MS.gene055141 | MS.gene75719 | 0.829321 | 5.49E-55 | -1.69E-46 |
| MS.gene055465 | MS.gene75719 | 0.823509 | 1.33E-53 | -1.69E-46 |
| MS.gene055466 | MS.gene75719 | 0.805717 | 1.17E-49 | -1.69E-46 |
| MS.gene055468 | MS.gene75719 | 0.825736 | 3.98E-54 | -1.69E-46 |
| MS.gene055469 | MS.gene75719 | 0.844447 | 7.52E-59 | -1.69E-46 |
| MS.gene055513 | MS.gene75719 | 0.823856 | 1.11E-53 | -1.69E-46 |
| MS.gene05574 | MS.gene75719 | 0.819442 | 1.16E-52 | -1.69E-46 |
| MS.gene056082 | MS.gene75719 | 0.811517 | 6.73E-51 | -1.69E-46 |
| MS.gene056308 | MS.gene75719 | 0.825926 | 3.59E-54 | -1.69E-46 |
| MS.gene056390 | MS.gene75719 | 0.813122 | 3.00E-51 | -1.69E-46 |
| MS.gene056677 | MS.gene75719 | 0.806561 | 7.75E-50 | -1.69E-46 |
| MS.gene057102 | MS.gene75719 | 0.823825 | 1.12E-53 | -1.69E-46 |
| MS.gene058330 | MS.gene75719 | 0.810841 | 9.43E-51 | -1.69E-46 |
| MS.gene058339 | MS.gene75719 | 0.811848 | 5.70E-51 | -1.69E-46 |
| MS.gene058492 | MS.gene75719 | 0.816953 | 4.24E-52 | -1.69E-46 |
| MS.gene058852 | MS.gene75719 | 0.818102 | 2.34E-52 | -1.69E-46 |
| MS.gene059170 | MS.gene75719 | 0.84944 | 3.23E-60 | -1.69E-46 |
| MS.gene059171 | MS.gene75719 | 0.801167 | 1.02E-48 | -1.69E-46 |
| MS.gene059227 | MS.gene75719 | 0.803345 | 3.64E-49 | -1.69E-46 |
| MS.gene060259 | MS.gene75719 | 0.800311 | 1.53E-48 | -1.69E-46 |
| MS.gene060932 | MS.gene75719 | 0.841061 | 5.97E-58 | -1.69E-46 |
| MS.gene061100 | MS.gene75719 | 0.852063 | 5.90E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75719.t1 | MTR_3g083470 | 95.833 | 72 | 2 | 1 | 1 | 71 | 154 | 225 | 2.18e-42 | 142 |
| MS.gene75719.t1 | MTR_3g083470 | 67.568 | 74 | 21 | 2 | 131 | 202 | 89 | 161 | 1.72e-17 | 77.8 |
| MS.gene75719.t1 | MTR_8g105230 | 69.014 | 71 | 21 | 1 | 1 | 70 | 107 | 177 | 6.18e-25 | 96.7 |
| MS.gene75719.t1 | MTR_1g032070 | 62.687 | 67 | 25 | 0 | 1 | 67 | 117 | 183 | 6.52e-23 | 91.7 |
| MS.gene75719.t1 | MTR_3g067610 | 49.180 | 61 | 31 | 0 | 4 | 64 | 75 | 135 | 2.29e-17 | 75.5 |
| MS.gene75719.t1 | MTR_1g046950 | 44.444 | 63 | 35 | 0 | 2 | 64 | 78 | 140 | 9.33e-16 | 71.2 |
| MS.gene75719.t1 | MTR_5g017510 | 45.588 | 68 | 37 | 0 | 2 | 69 | 120 | 187 | 1.15e-15 | 72.0 |
| MS.gene75719.t1 | MTR_5g017560 | 45.588 | 68 | 37 | 0 | 2 | 69 | 120 | 187 | 1.15e-15 | 72.0 |
| MS.gene75719.t1 | MTR_5g079340 | 44.262 | 61 | 34 | 0 | 4 | 64 | 77 | 137 | 4.04e-15 | 69.7 |
| MS.gene75719.t1 | MTR_1g047100 | 44.444 | 63 | 35 | 0 | 2 | 64 | 77 | 139 | 5.05e-15 | 69.3 |
| MS.gene75719.t1 | MTR_5g017550 | 44.118 | 68 | 38 | 0 | 2 | 69 | 119 | 186 | 8.45e-15 | 69.7 |
| MS.gene75719.t1 | MTR_8g107110 | 43.284 | 67 | 38 | 0 | 4 | 70 | 97 | 163 | 3.52e-14 | 67.8 |
| MS.gene75719.t1 | MTR_3g083460 | 60.417 | 48 | 19 | 0 | 18 | 65 | 40 | 87 | 1.31e-13 | 64.3 |
| MS.gene75719.t1 | MTR_4g103655 | 45.312 | 64 | 35 | 0 | 1 | 64 | 76 | 139 | 3.58e-13 | 64.3 |
| MS.gene75719.t1 | MTR_4g103650 | 45.312 | 64 | 35 | 0 | 1 | 64 | 72 | 135 | 4.04e-13 | 64.3 |
| MS.gene75719.t1 | MTR_4g103630 | 43.750 | 64 | 36 | 0 | 1 | 64 | 76 | 139 | 1.11e-12 | 63.2 |
| MS.gene75719.t1 | MTR_7g090450 | 48.438 | 64 | 32 | 1 | 2 | 64 | 149 | 212 | 1.21e-12 | 64.7 |
| MS.gene75719.t1 | MTR_7g087610 | 50.820 | 61 | 30 | 0 | 4 | 64 | 13 | 73 | 1.27e-12 | 63.2 |
| MS.gene75719.t1 | MTR_6g079570 | 41.270 | 63 | 37 | 0 | 4 | 66 | 100 | 162 | 1.49e-12 | 63.5 |
| MS.gene75719.t1 | MTR_5g088320 | 50.820 | 61 | 30 | 0 | 4 | 64 | 13 | 73 | 1.61e-12 | 62.8 |
| MS.gene75719.t1 | MTR_6g025320 | 50.820 | 61 | 30 | 0 | 4 | 64 | 13 | 73 | 1.85e-12 | 62.8 |
| MS.gene75719.t1 | MTR_4g067270 | 43.284 | 67 | 36 | 1 | 2 | 66 | 77 | 143 | 2.11e-12 | 62.4 |
| MS.gene75719.t1 | MTR_6g007613 | 38.272 | 81 | 47 | 1 | 2 | 79 | 28 | 108 | 4.40e-12 | 62.0 |
| MS.gene75719.t1 | MTR_8g036075 | 50.000 | 64 | 31 | 1 | 2 | 64 | 128 | 191 | 4.60e-12 | 62.8 |
| MS.gene75719.t1 | MTR_1g076650 | 43.284 | 67 | 38 | 0 | 2 | 68 | 84 | 150 | 6.06e-12 | 61.2 |
| MS.gene75719.t1 | MTR_7g074020 | 38.272 | 81 | 47 | 1 | 2 | 79 | 29 | 109 | 6.15e-12 | 61.6 |
| MS.gene75719.t1 | MTR_6g007613 | 38.272 | 81 | 47 | 1 | 2 | 79 | 28 | 108 | 7.54e-12 | 61.6 |
| MS.gene75719.t1 | MTR_7g074020 | 38.272 | 81 | 47 | 1 | 2 | 79 | 29 | 109 | 8.25e-12 | 61.6 |
| MS.gene75719.t1 | MTR_5g008695 | 41.667 | 60 | 35 | 0 | 4 | 63 | 53 | 112 | 9.46e-12 | 60.1 |
| MS.gene75719.t1 | MTR_7g075040 | 46.875 | 64 | 33 | 1 | 2 | 64 | 127 | 190 | 1.60e-11 | 61.2 |
| MS.gene75719.t1 | MTR_1g041285 | 42.188 | 64 | 37 | 0 | 2 | 65 | 84 | 147 | 5.00e-11 | 58.9 |
| MS.gene75719.t1 | MTR_1g071150 | 42.188 | 64 | 37 | 0 | 2 | 65 | 83 | 146 | 6.03e-11 | 58.5 |
| MS.gene75719.t1 | MTR_0168s0120 | 40.909 | 66 | 39 | 0 | 2 | 67 | 48 | 113 | 6.64e-11 | 57.8 |
| MS.gene75719.t1 | MTR_6g025320 | 40.909 | 66 | 39 | 0 | 2 | 67 | 48 | 113 | 6.64e-11 | 57.8 |
| MS.gene75719.t1 | MTR_3g088655 | 43.077 | 65 | 35 | 1 | 3 | 65 | 91 | 155 | 8.79e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75719.t1 | AT1G24620 | 63.380 | 71 | 25 | 1 | 1 | 70 | 108 | 178 | 2.94e-24 | 94.7 |
| MS.gene75719.t1 | AT1G66400 | 49.231 | 65 | 33 | 0 | 1 | 65 | 88 | 152 | 1.48e-18 | 79.0 |
| MS.gene75719.t1 | AT5G37770 | 45.312 | 64 | 35 | 0 | 1 | 64 | 92 | 155 | 1.70e-16 | 73.9 |
| MS.gene75719.t1 | AT5G37770 | 45.312 | 64 | 35 | 0 | 1 | 64 | 92 | 155 | 1.70e-16 | 73.9 |
| MS.gene75719.t1 | AT2G43290 | 49.254 | 67 | 32 | 1 | 2 | 66 | 142 | 208 | 1.77e-14 | 69.7 |
| MS.gene75719.t1 | AT1G05990 | 46.377 | 69 | 35 | 1 | 4 | 70 | 80 | 148 | 2.31e-14 | 67.8 |
| MS.gene75719.t1 | AT1G18210 | 44.262 | 61 | 34 | 0 | 4 | 64 | 93 | 153 | 2.55e-14 | 68.2 |
| MS.gene75719.t1 | AT1G18210 | 44.262 | 61 | 34 | 0 | 4 | 64 | 93 | 153 | 2.55e-14 | 68.2 |
| MS.gene75719.t1 | AT1G73630 | 35.211 | 71 | 46 | 0 | 4 | 74 | 90 | 160 | 7.00e-13 | 64.3 |
| MS.gene75719.t1 | AT4G03290 | 46.269 | 67 | 34 | 1 | 4 | 68 | 82 | 148 | 1.13e-12 | 63.5 |
| MS.gene75719.t1 | AT5G37780 | 50.820 | 61 | 30 | 0 | 4 | 64 | 13 | 73 | 1.54e-12 | 63.2 |
| MS.gene75719.t1 | AT5G37780 | 42.424 | 66 | 38 | 0 | 2 | 67 | 84 | 149 | 6.24e-11 | 58.5 |
| MS.gene75719.t1 | AT1G66410 | 50.820 | 61 | 30 | 0 | 4 | 64 | 13 | 73 | 1.54e-12 | 63.2 |
| MS.gene75719.t1 | AT1G66410 | 42.424 | 66 | 38 | 0 | 2 | 67 | 84 | 149 | 6.24e-11 | 58.5 |
| MS.gene75719.t1 | AT3G56800 | 50.820 | 61 | 30 | 0 | 4 | 64 | 13 | 73 | 1.71e-12 | 62.8 |
| MS.gene75719.t1 | AT2G27030 | 50.820 | 61 | 30 | 0 | 4 | 64 | 13 | 73 | 1.71e-12 | 62.8 |
| MS.gene75719.t1 | AT2G41110 | 50.820 | 61 | 30 | 0 | 4 | 64 | 13 | 73 | 1.71e-12 | 62.8 |
| MS.gene75719.t1 | AT5G21274 | 50.820 | 61 | 30 | 0 | 4 | 64 | 13 | 73 | 1.86e-12 | 62.8 |
| MS.gene75719.t1 | AT3G43810 | 50.820 | 61 | 30 | 0 | 4 | 64 | 13 | 73 | 1.93e-12 | 62.8 |
| MS.gene75719.t1 | AT5G17470 | 42.623 | 61 | 35 | 0 | 4 | 64 | 78 | 138 | 2.22e-12 | 62.4 |
| MS.gene75719.t1 | AT3G59440 | 45.205 | 73 | 37 | 2 | 3 | 72 | 123 | 195 | 2.28e-12 | 63.5 |
| MS.gene75719.t1 | AT2G36180 | 35.294 | 68 | 44 | 0 | 4 | 71 | 77 | 144 | 2.46e-12 | 62.4 |
| MS.gene75719.t1 | AT3G43810 | 50.820 | 61 | 30 | 0 | 4 | 64 | 13 | 73 | 4.12e-12 | 63.2 |
| MS.gene75719.t1 | AT2G27030 | 50.820 | 61 | 30 | 0 | 4 | 64 | 13 | 73 | 4.12e-12 | 62.4 |
| MS.gene75719.t1 | AT2G27030 | 37.333 | 75 | 47 | 0 | 2 | 76 | 84 | 158 | 1.36e-11 | 61.2 |
| MS.gene75719.t1 | AT1G66410 | 42.424 | 66 | 38 | 0 | 2 | 67 | 48 | 113 | 1.35e-11 | 59.7 |
| MS.gene75719.t1 | AT1G66410 | 42.424 | 66 | 38 | 0 | 2 | 67 | 48 | 113 | 1.35e-11 | 59.7 |
| MS.gene75719.t1 | AT2G15680 | 38.806 | 67 | 40 | 1 | 3 | 69 | 121 | 186 | 1.64e-11 | 60.8 |
| MS.gene75719.t1 | AT4G12860 | 45.161 | 62 | 32 | 1 | 7 | 66 | 82 | 143 | 1.79e-11 | 60.1 |
| MS.gene75719.t1 | AT3G07490 | 45.161 | 62 | 32 | 1 | 7 | 66 | 82 | 143 | 1.98e-11 | 60.1 |
| MS.gene75719.t1 | AT3G10190 | 50.000 | 62 | 30 | 1 | 4 | 64 | 144 | 205 | 5.40e-11 | 60.1 |
| MS.gene75719.t1 | AT5G37780 | 42.424 | 66 | 38 | 0 | 2 | 67 | 71 | 136 | 5.64e-11 | 58.5 |
| MS.gene75719.t1 | AT3G43810 | 40.909 | 66 | 39 | 0 | 2 | 67 | 48 | 113 | 6.95e-11 | 57.8 |
| MS.gene75719.t1 | AT3G43810 | 40.909 | 66 | 39 | 0 | 2 | 67 | 48 | 113 | 6.95e-11 | 57.8 |
| MS.gene75719.t1 | AT1G66410 | 42.424 | 66 | 38 | 0 | 2 | 67 | 94 | 159 | 6.99e-11 | 58.9 |
| MS.gene75719.t1 | AT5G37780 | 42.424 | 66 | 38 | 0 | 2 | 67 | 99 | 164 | 9.26e-11 | 58.5 |
Find 43 sgRNAs with CRISPR-Local
Find 144 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACCTTGCCGTCACCTTCTT+TGG | 0.219243 | 3.3:+70113614 | None:intergenic |
| TTTGATGTTGGACTCTATTA+TGG | 0.222707 | 3.3:+70115516 | None:intergenic |
| GATGAATGTGAAAGAATTAT+TGG | 0.254128 | 3.3:-70115608 | MS.gene75719:CDS |
| AATTCTTTCACATTCATCTA+AGG | 0.287517 | 3.3:+70115613 | None:intergenic |
| ATCTGATCTAAGAGCGATAA+TGG | 0.288423 | 3.3:-70113586 | MS.gene75719:CDS |
| AGAGGAAATGGAAAACTTAA+AGG | 0.303204 | 3.3:-70115726 | None:intergenic |
| TAACCTTGAAACTGGATTAC+TGG | 0.330482 | 3.3:+70114137 | None:intergenic |
| TGGATTACTGGAGTTTGAAT+TGG | 0.335217 | 3.3:+70114149 | None:intergenic |
| AAAGTCAGGCCAAGTATAAA+TGG | 0.337545 | 3.3:+70114196 | None:intergenic |
| CGAGGTACTAACCTTGAAAC+TGG | 0.337964 | 3.3:+70114129 | None:intergenic |
| TGCTTAGCTCAATAAATTCT+TGG | 0.338403 | 3.3:+70113514 | None:intergenic |
| CAATAGTAGCACCTTGGAAT+TGG | 0.366331 | 3.3:-70113667 | MS.gene75719:CDS |
| AAGAGCGATAATGGGAAAGT+TGG | 0.422840 | 3.3:-70113577 | MS.gene75719:CDS |
| ATATATAATTATCGATTTCA+AGG | 0.424075 | 3.3:-70113732 | MS.gene75719:CDS |
| TTATTGTGGCCATTTATACT+TGG | 0.446233 | 3.3:-70114205 | MS.gene75719:CDS |
| AGAAACAATGGCATCGCTAA+TGG | 0.452342 | 3.3:-70113705 | MS.gene75719:CDS |
| GAGGAAATGGAAAACTTAAA+GGG | 0.465725 | 3.3:-70115725 | None:intergenic |
| AATATATTTATGCAAAAGTC+AGG | 0.467136 | 3.3:+70114182 | None:intergenic |
| ATAATGGGAAAGTTGGGTAT+AGG | 0.468705 | 3.3:-70113570 | MS.gene75719:CDS |
| ACTCCAGTAATCCAGTTTCA+AGG | 0.469240 | 3.3:-70114140 | MS.gene75719:intron |
| AAGCGTATTAAGCTCCTTTG+CGG | 0.477365 | 3.3:+70115661 | None:intergenic |
| TCTGATCTAAGAGCGATAAT+GGG | 0.494788 | 3.3:-70113585 | MS.gene75719:CDS |
| AGAGCGATAATGGGAAAGTT+GGG | 0.504415 | 3.3:-70113576 | MS.gene75719:CDS |
| TATACAGGGCATCATTATTG+TGG | 0.520951 | 3.3:-70114219 | MS.gene75719:intron |
| AATACGCTTATGAGAAGCAT+TGG | 0.523714 | 3.3:-70115647 | MS.gene75719:CDS |
| AAGAAATTGGAGACCAAAGA+AGG | 0.535558 | 3.3:-70113627 | MS.gene75719:CDS |
| GAGTTTGAATTGGACTCCAT+CGG | 0.545341 | 3.3:+70114159 | None:intergenic |
| TAGCACCTTGGAATTGGAGA+TGG | 0.560673 | 3.3:-70113661 | MS.gene75719:CDS |
| TTTGCATAAATATATTCCGA+TGG | 0.564718 | 3.3:-70114175 | MS.gene75719:CDS |
| CTCTTCAGTTTCAGAATGTG+TGG | 0.566849 | 3.3:+70113491 | None:intergenic |
| TTGATAGTGATGGTGATGGT+AGG | 0.571325 | 3.3:-70115580 | MS.gene75719:CDS |
| GGCATCGCTAATGGCGACGT+TGG | 0.580456 | 3.3:-70113696 | MS.gene75719:CDS |
| GTGTATGACATTGATGGCGA+TGG | 0.590310 | 3.3:-70115692 | MS.gene75719:CDS |
| CACACATTCTGAAACTGAAG+AGG | 0.599111 | 3.3:-70113490 | MS.gene75719:CDS |
| GACCAAAGAAGGTGACGGCA+AGG | 0.600759 | 3.3:-70113616 | MS.gene75719:CDS |
| ATTGGTCGTGTTGATAGTGA+TGG | 0.614485 | 3.3:-70115590 | MS.gene75719:CDS |
| TGATGACAATAGTAGCACCT+TGG | 0.620972 | 3.3:-70113673 | MS.gene75719:CDS |
| CGATGGTTTCATCACCGCAA+AGG | 0.626557 | 3.3:-70115675 | MS.gene75719:CDS |
| TTGGAGACCAAAGAAGGTGA+CGG | 0.633691 | 3.3:-70113621 | MS.gene75719:CDS |
| CGTGTTGATAGTGATGGTGA+TGG | 0.638546 | 3.3:-70115584 | MS.gene75719:CDS |
| TTTCAAGGAGAGAGAAACAA+TGG | 0.649363 | 3.3:-70113717 | MS.gene75719:CDS |
| ATAGGAGACGATGTTGACAA+CGG | 0.711944 | 3.3:-70113552 | MS.gene75719:CDS |
| GGAGACGATGTTGACAACGG+AGG | 0.719496 | 3.3:-70113549 | MS.gene75719:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATTGAATTTATTATACA+GGG | - | chr3.3:70114947-70114966 | MS.gene75719:intron | 10.0% |
| !!! | TTTTTTTTTTTACAAAAAAC+CGG | + | chr3.3:70114140-70114159 | None:intergenic | 10.0% |
| !! | CTATATTGAATTTATTATAC+AGG | - | chr3.3:70114946-70114965 | MS.gene75719:intron | 15.0% |
| !! | TGTTGTTAACAAATTAAAAA+AGG | - | chr3.3:70114793-70114812 | MS.gene75719:intron | 15.0% |
| !! | TTATTGTATATTTCTTACTA+TGG | - | chr3.3:70115257-70115276 | MS.gene75719:intron | 15.0% |
| !!! | AATTGTGTTGTTATTTTTTT+TGG | - | chr3.3:70113766-70113785 | MS.gene75719:intron | 15.0% |
| !!! | ATATATAATTATCGATTTCA+AGG | - | chr3.3:70115448-70115467 | MS.gene75719:intron | 15.0% |
| !! | AAACAGTTTATATAAGATGT+TGG | + | chr3.3:70115170-70115189 | None:intergenic | 20.0% |
| !! | AATATATTTATGCAAAAGTC+AGG | + | chr3.3:70115001-70115020 | None:intergenic | 20.0% |
| !! | ATCAAAATTAGATACATACT+AGG | - | chr3.3:70114201-70114220 | MS.gene75719:CDS | 20.0% |
| !! | ATGACTTATATTCAAATGTT+TGG | - | chr3.3:70114758-70114777 | MS.gene75719:intron | 20.0% |
| !! | GATAATCGTTAATTTAGTTA+AGG | + | chr3.3:70114405-70114424 | None:intergenic | 20.0% |
| !! | GTTTCTATTAGAAAAAACAA+TGG | - | chr3.3:70114501-70114520 | MS.gene75719:intron | 20.0% |
| !! | TCAAAATTAGATACATACTA+GGG | - | chr3.3:70114202-70114221 | MS.gene75719:CDS | 20.0% |
| !!! | ATATAAACTGTTTACCATTT+TGG | - | chr3.3:70115177-70115196 | MS.gene75719:intron | 20.0% |
| !!! | TATTTTAAAACAACCCATTA+GGG | - | chr3.3:70115291-70115310 | MS.gene75719:intron | 20.0% |
| !!! | TGATGTTTTAGATATTGTTT+AGG | + | chr3.3:70114827-70114846 | None:intergenic | 20.0% |
| !!! | TTTTTTTTTTGATTTCCCTT+TGG | - | chr3.3:70115104-70115123 | MS.gene75719:intron | 20.0% |
| ! | AACAATGGTATAGTAATAGT+TGG | - | chr3.3:70114516-70114535 | MS.gene75719:intron | 25.0% |
| ! | AATCGTTAATTTAGTTAAGG+TGG | + | chr3.3:70114402-70114421 | None:intergenic | 25.0% |
| ! | AATTCTTTCACATTCATCTA+AGG | + | chr3.3:70113570-70113589 | None:intergenic | 25.0% |
| ! | ACAAAAAGAATTGTTTGCAA+TGG | + | chr3.3:70114722-70114741 | None:intergenic | 25.0% |
| ! | ATGGATCATTAAAAAAAGAG+AGG | - | chr3.3:70113890-70113909 | MS.gene75719:intron | 25.0% |
| ! | CATGTTCATGACTAAATAAT+TGG | + | chr3.3:70114292-70114311 | None:intergenic | 25.0% |
| ! | GATGAATGTGAAAGAATTAT+TGG | - | chr3.3:70113572-70113591 | MS.gene75719:CDS | 25.0% |
| ! | GGAAAAAATCTTCAAGAAAT+TGG | - | chr3.3:70115540-70115559 | MS.gene75719:CDS | 25.0% |
| ! | GGTGAAGATGTTAAAAAAAA+TGG | + | chr3.3:70114170-70114189 | None:intergenic | 25.0% |
| ! | TAAGAAATATACAATAACCG+CGG | + | chr3.3:70115254-70115273 | None:intergenic | 25.0% |
| ! | TCATGACTAAATAATTGGTT+CGG | + | chr3.3:70114287-70114306 | None:intergenic | 25.0% |
| ! | TGTTGATGTTATTCATGATA+TGG | - | chr3.3:70113809-70113828 | MS.gene75719:intron | 25.0% |
| ! | TTTGCATAAATATATTCCGA+TGG | - | chr3.3:70115005-70115024 | MS.gene75719:intron | 25.0% |
| !!! | CTATTTTAAAACAACCCATT+AGG | - | chr3.3:70115290-70115309 | MS.gene75719:intron | 25.0% |
| !!! | GACAAGATCAAATTGATTTT+AGG | - | chr3.3:70114371-70114390 | MS.gene75719:intron | 25.0% |
| !!! | GATTTTAGAATCATGATGAT+GGG | - | chr3.3:70113635-70113654 | MS.gene75719:CDS | 25.0% |
| !!! | GTGTTGTTATTTTTTTTGGT+TGG | - | chr3.3:70113770-70113789 | MS.gene75719:intron | 25.0% |
| !!! | TAATTTTGATTTGCCAATCA+TGG | + | chr3.3:70114191-70114210 | None:intergenic | 25.0% |
| AATAAGGATGAGATGATACA+AGG | - | chr3.3:70114349-70114368 | MS.gene75719:intron | 30.0% | |
| ACTAGGGATGTATTAGAAAT+AGG | - | chr3.3:70114218-70114237 | MS.gene75719:intron | 30.0% | |
| AGAAATGTCAAGTTTGATGT+TGG | + | chr3.3:70113679-70113698 | None:intergenic | 30.0% | |
| AGTAAGAGTAAGACTGAAAT+TGG | + | chr3.3:70115331-70115350 | None:intergenic | 30.0% | |
| ATTTCAGTCTTACTCTTACT+AGG | - | chr3.3:70115331-70115350 | MS.gene75719:intron | 30.0% | |
| GACGTAATACATGTTCAATA+AGG | - | chr3.3:70114333-70114352 | MS.gene75719:intron | 30.0% | |
| GGATGTATTAGAAATAGGTT+AGG | - | chr3.3:70114223-70114242 | MS.gene75719:intron | 30.0% | |
| GTTATTGAAAAGAAAGTTCC+AGG | + | chr3.3:70114903-70114922 | None:intergenic | 30.0% | |
| TATAAATACAGTGAGCCATA+AGG | + | chr3.3:70115419-70115438 | None:intergenic | 30.0% | |
| TATTAGAAATAGGTTAGGTC+TGG | - | chr3.3:70114228-70114247 | MS.gene75719:intron | 30.0% | |
| TCATAATTTGAACCCAGAAA+TGG | + | chr3.3:70114448-70114467 | None:intergenic | 30.0% | |
| TCGGTAATTGTCATAAAACA+CGG | - | chr3.3:70113997-70114016 | MS.gene75719:intron | 30.0% | |
| TGAACTAGTGTTTAAAGACT+TGG | - | chr3.3:70115144-70115163 | MS.gene75719:intron | 30.0% | |
| TGCTATACTATGCATTATTG+TGG | - | chr3.3:70115077-70115096 | MS.gene75719:intron | 30.0% | |
| TGCTTAGCTCAATAAATTCT+TGG | + | chr3.3:70115669-70115688 | None:intergenic | 30.0% | |
| TTATTGTGGCCATTTATACT+TGG | - | chr3.3:70114975-70114994 | MS.gene75719:intron | 30.0% | |
| TTTAACATCTTCACCATGAT+TGG | - | chr3.3:70114175-70114194 | MS.gene75719:CDS | 30.0% | |
| TTTGATGTTGGACTCTATTA+TGG | + | chr3.3:70113667-70113686 | None:intergenic | 30.0% | |
| ! | GTCTTCTTTACTTTATTTCC+TGG | - | chr3.3:70114882-70114901 | MS.gene75719:intron | 30.0% |
| ! | GTTCGGTCTAATTTAAGATT+GGG | + | chr3.3:70114270-70114289 | None:intergenic | 30.0% |
| ! | TGCTAATATTTGCCATTTCT+GGG | - | chr3.3:70114433-70114452 | MS.gene75719:intron | 30.0% |
| ! | TTGCTAATATTTGCCATTTC+TGG | - | chr3.3:70114432-70114451 | MS.gene75719:intron | 30.0% |
| ! | TTTTCTGTGTATGACATTGA+TGG | - | chr3.3:70113482-70113501 | MS.gene75719:CDS | 30.0% |
| !!! | GGATTTTAGAATCATGATGA+TGG | - | chr3.3:70113634-70113653 | MS.gene75719:CDS | 30.0% |
| !!! | TTTTGATTTCCCTTTGGAAA+AGG | - | chr3.3:70115110-70115129 | MS.gene75719:intron | 30.0% |
| AAAGTCAGGCCAAGTATAAA+TGG | + | chr3.3:70114987-70115006 | None:intergenic | 35.0% | |
| AACTATTGCAATACTCCATG+TGG | - | chr3.3:70114571-70114590 | MS.gene75719:intron | 35.0% | |
| AAGAAATTGGAGACCAAAGA+AGG | - | chr3.3:70115553-70115572 | MS.gene75719:CDS | 35.0% | |
| AATGAAACGTTCAACCCTAA+TGG | + | chr3.3:70115308-70115327 | None:intergenic | 35.0% | |
| AATTGTCATAAAACACGGGA+GGG | - | chr3.3:70114002-70114021 | MS.gene75719:intron | 35.0% | |
| ACGAGAGTTAAGTACTAACA+TGG | - | chr3.3:70113871-70113890 | MS.gene75719:intron | 35.0% | |
| ATCTGATCTAAGAGCGATAA+TGG | - | chr3.3:70115594-70115613 | MS.gene75719:CDS | 35.0% | |
| ATGAAACGTTCAACCCTAAT+GGG | + | chr3.3:70115307-70115326 | None:intergenic | 35.0% | |
| CATGATATGGTATGTAGAAG+TGG | - | chr3.3:70113822-70113841 | MS.gene75719:intron | 35.0% | |
| CGGTAATTGTCATAAAACAC+GGG | - | chr3.3:70113998-70114017 | MS.gene75719:intron | 35.0% | |
| GTAGAATGAATTTGATCCAG+TGG | + | chr3.3:70113746-70113765 | None:intergenic | 35.0% | |
| GTCGAGTTCAATTAGATTCA+CGG | + | chr3.3:70114316-70114335 | None:intergenic | 35.0% | |
| GTTGCTTGAAATAAGTAGCA+AGG | + | chr3.3:70115377-70115396 | None:intergenic | 35.0% | |
| TAACCTTGAAACTGGATTAC+TGG | + | chr3.3:70115046-70115065 | None:intergenic | 35.0% | |
| TAATTGTCATAAAACACGGG+AGG | - | chr3.3:70114001-70114020 | MS.gene75719:intron | 35.0% | |
| TAGCATGACTGCTTAAAGTT+CGG | - | chr3.3:70113978-70113997 | MS.gene75719:intron | 35.0% | |
| TCTGATCTAAGAGCGATAAT+GGG | - | chr3.3:70115595-70115614 | MS.gene75719:CDS | 35.0% | |
| TGGATTACTGGAGTTTGAAT+TGG | + | chr3.3:70115034-70115053 | None:intergenic | 35.0% | |
| TGTTTGCAATGGTTGAAAAG+TGG | + | chr3.3:70114711-70114730 | None:intergenic | 35.0% | |
| TTTCAAGGAGAGAGAAACAA+TGG | - | chr3.3:70115463-70115482 | MS.gene75719:intron | 35.0% | |
| ! | AATACGCTTATGAGAAGCAT+TGG | - | chr3.3:70113533-70113552 | MS.gene75719:CDS | 35.0% |
| ! | ATAATGGGAAAGTTGGGTAT+AGG | - | chr3.3:70115610-70115629 | MS.gene75719:CDS | 35.0% |
| ! | ATAGGTCGTATAGTGTTGTT+TGG | + | chr3.3:70115224-70115243 | None:intergenic | 35.0% |
| ! | CAACATTGCTATTGTCACTT+TGG | - | chr3.3:70115393-70115412 | MS.gene75719:intron | 35.0% |
| ! | GGTTCGGTCTAATTTAAGAT+TGG | + | chr3.3:70114271-70114290 | None:intergenic | 35.0% |
| ! | TATACAGGGCATCATTATTG+TGG | - | chr3.3:70114961-70114980 | MS.gene75719:intron | 35.0% |
| ! | TTTGGAAAGCTTGTTTACCA+AGG | + | chr3.3:70115206-70115225 | None:intergenic | 35.0% |
| ! | TTTTCCAAACCATCGCAAAA+TGG | - | chr3.3:70114609-70114628 | MS.gene75719:intron | 35.0% |
| ! | TTTTTCCATCTCCAATTCCA+AGG | + | chr3.3:70115527-70115546 | None:intergenic | 35.0% |
| !! | GTTGAAAAGTGGATTTGCTA+CGG | + | chr3.3:70114700-70114719 | None:intergenic | 35.0% |
| !! | TGATGGTAGGATTGATTTTG+AGG | - | chr3.3:70113613-70113632 | MS.gene75719:CDS | 35.0% |
| AAGCGTATTAAGCTCCTTTG+CGG | + | chr3.3:70113522-70113541 | None:intergenic | 40.0% | |
| AAGTAGCAAGGCTAAACATG+AGG | + | chr3.3:70115365-70115384 | None:intergenic | 40.0% | |
| ACCAAGCGAATAACTGTGAT+TGG | + | chr3.3:70114041-70114060 | None:intergenic | 40.0% | |
| ACCAATCACAGTTATTCGCT+TGG | - | chr3.3:70114037-70114056 | MS.gene75719:intron | 40.0% | |
| ACTCCAGTAATCCAGTTTCA+AGG | - | chr3.3:70115040-70115059 | MS.gene75719:intron | 40.0% | |
| AGAAACAATGGCATCGCTAA+TGG | - | chr3.3:70115475-70115494 | MS.gene75719:intron | 40.0% | |
| ATAGGAGACGATGTTGACAA+CGG | - | chr3.3:70115628-70115647 | MS.gene75719:CDS | 40.0% | |
| ATGCATAGTATAGCAAACCG+AGG | + | chr3.3:70115072-70115091 | None:intergenic | 40.0% | |
| CAAGACGAAAACGTTATAGC+CGG | - | chr3.3:70114118-70114137 | MS.gene75719:intron | 40.0% | |
| CAATAGTAGCACCTTGGAAT+TGG | - | chr3.3:70115513-70115532 | MS.gene75719:intron | 40.0% | |
| CAGTTTCAAGGTTAGTACCT+CGG | - | chr3.3:70115052-70115071 | MS.gene75719:intron | 40.0% | |
| CGAGAATATATCTCTCGAGA+TGG | - | chr3.3:70114064-70114083 | MS.gene75719:intron | 40.0% | |
| CTCTTCAGTTTCAGAATGTG+TGG | + | chr3.3:70115692-70115711 | None:intergenic | 40.0% | |
| GAAATAGGTTAGGTCTGGTT+TGG | - | chr3.3:70114233-70114252 | MS.gene75719:intron | 40.0% | |
| GAGTTTGAATTGGACTCCAT+CGG | + | chr3.3:70115024-70115043 | None:intergenic | 40.0% | |
| TATCCATAGACAATTGCCAC+TGG | - | chr3.3:70113727-70113746 | MS.gene75719:CDS | 40.0% | |
| TGATGACAATAGTAGCACCT+TGG | - | chr3.3:70115507-70115526 | MS.gene75719:intron | 40.0% | |
| ! | GCTTTTGCTCCTTTTCCAAA+GGG | + | chr3.3:70115122-70115141 | None:intergenic | 40.0% |
| ! | TAGACCATTTTGCGATGGTT+TGG | + | chr3.3:70114616-70114635 | None:intergenic | 40.0% |
| !! | AAGAGCGATAATGGGAAAGT+TGG | - | chr3.3:70115603-70115622 | MS.gene75719:CDS | 40.0% |
| !! | AGAGCGATAATGGGAAAGTT+GGG | - | chr3.3:70115604-70115623 | MS.gene75719:CDS | 40.0% |
| !! | ATTGGTCGTGTTGATAGTGA+TGG | - | chr3.3:70113590-70113609 | MS.gene75719:CDS | 40.0% |
| !! | CACACATTCTGAAACTGAAG+AGG | - | chr3.3:70115690-70115709 | MS.gene75719:CDS | 40.0% |
| !! | CTATTGTCACTTTGGCCTTA+TGG | - | chr3.3:70115401-70115420 | MS.gene75719:intron | 40.0% |
| !! | TTGATAGTGATGGTGATGGT+AGG | - | chr3.3:70113600-70113619 | MS.gene75719:CDS | 40.0% |
| AATAACCGCGGCGATGAAAT+AGG | + | chr3.3:70115242-70115261 | None:intergenic | 45.0% | |
| CCATGTGGTAGTTGTAACGA+CGG | - | chr3.3:70114586-70114605 | MS.gene75719:intron | 45.0% | |
| GATCCAGTGGCAATTGTCTA+TGG | + | chr3.3:70113733-70113752 | None:intergenic | 45.0% | |
| GTTTACCAAGGCAGCCAAAA+TGG | + | chr3.3:70115194-70115213 | None:intergenic | 45.0% | |
| TAGCACCTTGGAATTGGAGA+TGG | - | chr3.3:70115519-70115538 | MS.gene75719:intron | 45.0% | |
| TTGGAGACCAAAGAAGGTGA+CGG | - | chr3.3:70115559-70115578 | MS.gene75719:CDS | 45.0% | |
| ! | CGAGGTACTAACCTTGAAAC+TGG | + | chr3.3:70115054-70115073 | None:intergenic | 45.0% |
| ! | CGCTTTTGCTCCTTTTCCAA+AGG | + | chr3.3:70115123-70115142 | None:intergenic | 45.0% |
| !! | CGTGTTGATAGTGATGGTGA+TGG | - | chr3.3:70113596-70113615 | MS.gene75719:CDS | 45.0% |
| !! | GTGTATGACATTGATGGCGA+TGG | - | chr3.3:70113488-70113507 | MS.gene75719:CDS | 45.0% |
| !!! | GTTTACCATTTTGGCTGCCT+TGG | - | chr3.3:70115186-70115205 | MS.gene75719:intron | 45.0% |
| ACACCGATATCCTCGATCGT+TGG | - | chr3.3:70114639-70114658 | MS.gene75719:intron | 50.0% | |
| ACCGATATCCTCGATCGTTG+GGG | - | chr3.3:70114641-70114660 | MS.gene75719:intron | 50.0% | |
| CACCGATATCCTCGATCGTT+GGG | - | chr3.3:70114640-70114659 | MS.gene75719:intron | 50.0% | |
| CCGTCGTTACAACTACCACA+TGG | + | chr3.3:70114589-70114608 | None:intergenic | 50.0% | |
| CGATGGTTTCATCACCGCAA+AGG | - | chr3.3:70113505-70113524 | MS.gene75719:CDS | 50.0% | |
| TACGACCTATTTCATCGCCG+CGG | - | chr3.3:70115234-70115253 | MS.gene75719:intron | 50.0% | |
| TCCCCAACGATCGAGGATAT+CGG | + | chr3.3:70114645-70114664 | None:intergenic | 50.0% | |
| TGTCATAAAACACGGGAGGG+TGG | - | chr3.3:70114005-70114024 | MS.gene75719:intron | 50.0% | |
| ! | GTCGCTAGACCATTTTGCGA+TGG | + | chr3.3:70114621-70114640 | None:intergenic | 50.0% |
| !! | TCGCAATGTGCCATTTGCGA+CGG | + | chr3.3:70114668-70114687 | None:intergenic | 50.0% |
| GACCTTGCCGTCACCTTCTT+TGG | + | chr3.3:70115569-70115588 | None:intergenic | 55.0% | |
| GGAGACGATGTTGACAACGG+AGG | - | chr3.3:70115631-70115650 | MS.gene75719:CDS | 55.0% | |
| ! | GACCAAAGAAGGTGACGGCA+AGG | - | chr3.3:70115564-70115583 | MS.gene75719:CDS | 55.0% |
| GGCATCGCTAATGGCGACGT+TGG | - | chr3.3:70115484-70115503 | MS.gene75719:intron | 60.0% | |
| TCGTTGGGGACCGTCGCAAA+TGG | - | chr3.3:70114655-70114674 | MS.gene75719:intron | 60.0% | |
| GCGACGGTCCCCAACGATCG+AGG | + | chr3.3:70114652-70114671 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 70113461 | 70115741 | 70113461 | ID=MS.gene75719 |
| chr3.3 | mRNA | 70113461 | 70115741 | 70113461 | ID=MS.gene75719.t1;Parent=MS.gene75719 |
| chr3.3 | exon | 70115529 | 70115741 | 70115529 | ID=MS.gene75719.t1.exon1;Parent=MS.gene75719.t1 |
| chr3.3 | CDS | 70115529 | 70115741 | 70115529 | ID=cds.MS.gene75719.t1;Parent=MS.gene75719.t1 |
| chr3.3 | exon | 70114141 | 70114234 | 70114141 | ID=MS.gene75719.t1.exon2;Parent=MS.gene75719.t1 |
| chr3.3 | CDS | 70114141 | 70114234 | 70114141 | ID=cds.MS.gene75719.t1;Parent=MS.gene75719.t1 |
| chr3.3 | exon | 70113461 | 70113762 | 70113461 | ID=MS.gene75719.t1.exon3;Parent=MS.gene75719.t1 |
| chr3.3 | CDS | 70113461 | 70113762 | 70113461 | ID=cds.MS.gene75719.t1;Parent=MS.gene75719.t1 |
| Gene Sequence |
| Protein sequence |
