AlfalfaGEDB | MS.gene80732

M. sativa cultivar XinJiangDaYe / MS.gene80732

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80732.t1	RHN63835.1	97.4	76	2	0	3	78	91	166	2.70E-34	154.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80732.t1	A0A2Z6LM17	97.4	76	2	0	3	78	91	166	2.0e-34	154.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene80732	0.865101	7.54E-65	-1.69E-46
MS.gene049159	MS.gene80732	0.819953	8.86E-53	-1.69E-46
MS.gene049254	MS.gene80732	0.818055	2.39E-52	-1.69E-46
MS.gene049350	MS.gene80732	0.813485	2.50E-51	-1.69E-46
MS.gene049368	MS.gene80732	0.832117	1.13E-55	-1.69E-46
MS.gene049404	MS.gene80732	0.808506	2.99E-50	-1.69E-46
MS.gene049499	MS.gene80732	0.822272	2.59E-53	-1.69E-46
MS.gene049585	MS.gene80732	0.804671	1.93E-49	-1.69E-46
MS.gene049711	MS.gene80732	0.839316	1.70E-57	-1.69E-46
MS.gene049744	MS.gene80732	0.815457	9.16E-52	-1.69E-46
MS.gene049807	MS.gene80732	0.826138	3.20E-54	-1.69E-46
MS.gene049983	MS.gene80732	0.850963	1.21E-60	-1.69E-46
MS.gene050221	MS.gene80732	0.801511	8.70E-49	-1.69E-46
MS.gene050224	MS.gene80732	0.812802	3.53E-51	-1.69E-46
MS.gene050348	MS.gene80732	0.812389	4.35E-51	-1.69E-46
MS.gene050414	MS.gene80732	0.820914	5.33E-53	-1.69E-46
MS.gene050551	MS.gene80732	0.853182	2.83E-61	-1.69E-46
MS.gene050559	MS.gene80732	0.803422	3.51E-49	-1.69E-46
MS.gene050561	MS.gene80732	0.806321	8.71E-50	-1.69E-46
MS.gene050562	MS.gene80732	0.806285	8.86E-50	-1.69E-46
MS.gene050646	MS.gene80732	0.828224	1.01E-54	-1.69E-46
MS.gene050654	MS.gene80732	0.819377	1.20E-52	-1.69E-46
MS.gene050657	MS.gene80732	0.831621	1.50E-55	-1.69E-46
MS.gene051102	MS.gene80732	0.811077	8.39E-51	-1.69E-46
MS.gene051198	MS.gene80732	0.825725	4.01E-54	-1.69E-46
MS.gene051201	MS.gene80732	0.800033	1.74E-48	-1.69E-46
MS.gene051279	MS.gene80732	0.854306	1.34E-61	-1.69E-46
MS.gene051744	MS.gene80732	0.842715	2.18E-58	-1.69E-46
MS.gene051800	MS.gene80732	0.832244	1.06E-55	-1.69E-46
MS.gene051852	MS.gene80732	0.814589	1.43E-51	-1.69E-46
MS.gene052037	MS.gene80732	0.842247	2.90E-58	-1.69E-46
MS.gene052118	MS.gene80732	0.802949	4.40E-49	-1.69E-46
MS.gene052239	MS.gene80732	-0.802321	5.93E-49	-1.69E-46
MS.gene052307	MS.gene80732	0.874138	8.53E-68	-1.69E-46
MS.gene052311	MS.gene80732	0.807566	4.75E-50	-1.69E-46
MS.gene052463	MS.gene80732	0.864475	1.18E-64	-1.69E-46
MS.gene052514	MS.gene80732	0.833831	4.25E-56	-1.69E-46
MS.gene052516	MS.gene80732	0.852538	4.32E-61	-1.69E-46
MS.gene052609	MS.gene80732	0.863502	2.38E-64	-1.69E-46
MS.gene052773	MS.gene80732	0.800593	1.34E-48	-1.69E-46
MS.gene053079	MS.gene80732	0.86883	4.88E-66	-1.69E-46
MS.gene053143	MS.gene80732	0.811273	7.60E-51	-1.69E-46
MS.gene053144	MS.gene80732	0.822013	2.97E-53	-1.69E-46
MS.gene053164	MS.gene80732	0.804025	2.63E-49	-1.69E-46
MS.gene053226	MS.gene80732	0.811416	7.08E-51	-1.69E-46
MS.gene05350	MS.gene80732	0.800815	1.21E-48	-1.69E-46
MS.gene053621	MS.gene80732	0.813358	2.67E-51	-1.69E-46
MS.gene054178	MS.gene80732	0.810626	1.05E-50	-1.69E-46
MS.gene05423	MS.gene80732	0.819912	9.05E-53	-1.69E-46
MS.gene054576	MS.gene80732	0.814608	1.41E-51	-1.69E-46
MS.gene054584	MS.gene80732	0.823609	1.26E-53	-1.69E-46
MS.gene05458	MS.gene80732	0.827775	1.30E-54	-1.69E-46
MS.gene054641	MS.gene80732	0.823693	1.21E-53	-1.69E-46
MS.gene054651	MS.gene80732	0.847068	1.46E-59	-1.69E-46
MS.gene054709	MS.gene80732	0.80312	4.06E-49	-1.69E-46
MS.gene054991	MS.gene80732	0.800507	1.40E-48	-1.69E-46
MS.gene055465	MS.gene80732	0.803604	3.22E-49	-1.69E-46
MS.gene055469	MS.gene80732	0.825005	5.93E-54	-1.69E-46
MS.gene055513	MS.gene80732	0.828515	8.61E-55	-1.69E-46
MS.gene05574	MS.gene80732	0.812295	4.55E-51	-1.69E-46
MS.gene055864	MS.gene80732	0.816346	5.80E-52	-1.69E-46
MS.gene055926	MS.gene80732	0.8067	7.24E-50	-1.69E-46
MS.gene056050	MS.gene80732	0.827099	1.89E-54	-1.69E-46
MS.gene056174	MS.gene80732	0.818673	1.73E-52	-1.69E-46
MS.gene056181	MS.gene80732	0.802267	6.08E-49	-1.69E-46
MS.gene056308	MS.gene80732	0.834347	3.16E-56	-1.69E-46
MS.gene056507	MS.gene80732	0.864606	1.08E-64	-1.69E-46
MS.gene056519	MS.gene80732	0.821058	4.94E-53	-1.69E-46
MS.gene056677	MS.gene80732	0.807928	3.98E-50	-1.69E-46
MS.gene056699	MS.gene80732	0.810258	1.26E-50	-1.69E-46
MS.gene05686	MS.gene80732	0.866356	3.03E-65	-1.69E-46
MS.gene05690	MS.gene80732	0.83495	2.23E-56	-1.69E-46
MS.gene057034	MS.gene80732	0.812222	4.73E-51	-1.69E-46
MS.gene057102	MS.gene80732	0.816615	5.05E-52	-1.69E-46
MS.gene057132	MS.gene80732	0.808544	2.94E-50	-1.69E-46
MS.gene057208	MS.gene80732	0.811253	7.68E-51	-1.69E-46
MS.gene057598	MS.gene80732	0.802342	5.87E-49	-1.69E-46
MS.gene057869	MS.gene80732	0.821479	3.95E-53	-1.69E-46
MS.gene057915	MS.gene80732	0.822065	2.89E-53	-1.69E-46
MS.gene058008	MS.gene80732	0.804414	2.19E-49	-1.69E-46
MS.gene058009	MS.gene80732	0.814411	1.56E-51	-1.69E-46
MS.gene058010	MS.gene80732	0.851745	7.27E-61	-1.69E-46
MS.gene058289	MS.gene80732	0.825728	4.00E-54	-1.69E-46
MS.gene058292	MS.gene80732	0.808239	3.41E-50	-1.69E-46
MS.gene058330	MS.gene80732	0.816848	4.48E-52	-1.69E-46
MS.gene058625	MS.gene80732	0.816842	4.49E-52	-1.69E-46
MS.gene058647	MS.gene80732	0.815826	7.58E-52	-1.69E-46
MS.gene058649	MS.gene80732	0.801929	7.14E-49	-1.69E-46
MS.gene058781	MS.gene80732	0.803262	3.79E-49	-1.69E-46
MS.gene058919	MS.gene80732	0.825473	4.60E-54	-1.69E-46
MS.gene058979	MS.gene80732	0.805402	1.36E-49	-1.69E-46
MS.gene059170	MS.gene80732	0.834734	2.53E-56	-1.69E-46
MS.gene059439	MS.gene80732	0.823196	1.58E-53	-1.69E-46
MS.gene059570	MS.gene80732	0.842919	1.93E-58	-1.69E-46
MS.gene059634	MS.gene80732	0.818281	2.13E-52	-1.69E-46
MS.gene059965	MS.gene80732	0.818656	1.75E-52	-1.69E-46
MS.gene060373	MS.gene80732	0.811425	7.05E-51	-1.69E-46
MS.gene060435	MS.gene80732	0.807449	5.03E-50	-1.69E-46
MS.gene06052	MS.gene80732	0.848333	6.55E-60	-1.69E-46
MS.gene060785	MS.gene80732	0.838753	2.38E-57	-1.69E-46
MS.gene060932	MS.gene80732	0.814149	1.78E-51	-1.69E-46
MS.gene060933	MS.gene80732	0.829125	6.13E-55	-1.69E-46
MS.gene060935	MS.gene80732	0.828317	9.61E-55	-1.69E-46
MS.gene060937	MS.gene80732	0.838294	3.13E-57	-1.69E-46
MS.gene061006	MS.gene80732	0.805544	1.27E-49	-1.69E-46
MS.gene061100	MS.gene80732	0.838194	3.32E-57	-1.69E-46
MS.gene061188	MS.gene80732	0.804739	1.87E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 11 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAGAACAAGAACACCATTC+AGG	0.401351	8.4:+16297801	MS.gene80732:CDS
AGGAAAAGAGGGAGAAGAAA+AGG	0.464405	8.4:+16297496	MS.gene80732:CDS
TGTACATATATATTCCTGAA+TGG	0.494787	8.4:-16297815	None:intergenic
TATGTACAAACTGTGGCAAA+AGG	0.503511	8.4:+16297830	MS.gene80732:CDS
TGAAGCTACGACAGGAAAAG+AGG	0.510943	8.4:+16297484	MS.gene80732:CDS
GGCAGATATATGATGTGGTT+AGG	0.581872	8.4:+16297737	MS.gene80732:intron
GAAGCTACGACAGGAAAAGA+GGG	0.609436	8.4:+16297485	MS.gene80732:CDS
CTGCTGTCTAGATTATGCAC+AGG	0.622608	8.4:+16297541	MS.gene80732:CDS
GCTACAGCTGAAGCTACGAC+AGG	0.624340	8.4:+16297476	MS.gene80732:CDS
GAATATATATGTACAAACTG+TGG	0.654824	8.4:+16297823	MS.gene80732:CDS
TTGTTGGCAGATATATGATG+TGG	0.670036	8.4:+16297732	MS.gene80732:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAAAACAACTGAATATCAT+TGG	-	chr8.4:16297606-16297625	None:intergenic	20.0%
!!	TTCAGATAATTCAATTGAAT+TGG	+	chr8.4:16297665-16297684	MS.gene80732:intron	20.0%
!	AATTGAATTATCTGAAAAGC+AGG	-	chr8.4:16297661-16297680	None:intergenic	25.0%
!	GAATATATATGTACAAACTG+TGG	+	chr8.4:16297823-16297842	MS.gene80732:CDS	25.0%
!	GAATTGGTACATCAAAATTT+GGG	+	chr8.4:16297681-16297700	MS.gene80732:intron	25.0%
!	TGAATTGGTACATCAAAATT+TGG	+	chr8.4:16297680-16297699	MS.gene80732:intron	25.0%
!	TGTACATATATATTCCTGAA+TGG	-	chr8.4:16297818-16297837	None:intergenic	25.0%
	ATTTGGGCTCTTTCATAAAT+AGG	+	chr8.4:16297697-16297716	MS.gene80732:intron	30.0%
!	TAGGATACTTGTGATTTTGT+TGG	+	chr8.4:16297716-16297735	MS.gene80732:intron	30.0%
!!	TTGTTTTATTGCAGAGAGAA+GGG	+	chr8.4:16297617-16297636	MS.gene80732:intron	30.0%
	TTGTTGGCAGATATATGATG+TGG	+	chr8.4:16297732-16297751	MS.gene80732:intron	35.0%
!!	GTTGTTTTATTGCAGAGAGA+AGG	+	chr8.4:16297616-16297635	MS.gene80732:intron	35.0%
	ACGCTGAAGAATACAATCAG+TGG	-	chr8.4:16297576-16297595	None:intergenic	40.0%
	AGAGAACAAGAACACCATTC+AGG	+	chr8.4:16297801-16297820	MS.gene80732:CDS	40.0%
	AGGAAAAGAGGGAGAAGAAA+AGG	+	chr8.4:16297496-16297515	MS.gene80732:CDS	40.0%
	GGCAGATATATGATGTGGTT+AGG	+	chr8.4:16297737-16297756	MS.gene80732:intron	40.0%
	CTGCTGTCTAGATTATGCAC+AGG	+	chr8.4:16297541-16297560	MS.gene80732:CDS	45.0%
	GAAGCTACGACAGGAAAAGA+GGG	+	chr8.4:16297485-16297504	MS.gene80732:CDS	45.0%
	TGAAGCTACGACAGGAAAAG+AGG	+	chr8.4:16297484-16297503	MS.gene80732:CDS	45.0%
	GCTACAGCTGAAGCTACGAC+AGG	+	chr8.4:16297476-16297495	MS.gene80732:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	16297436	16297851	16297436	ID=MS.gene80732
chr8.4	mRNA	16297436	16297851	16297436	ID=MS.gene80732.t1;Parent=MS.gene80732
chr8.4	exon	16297436	16297562	16297436	ID=MS.gene80732.t1.exon1;Parent=MS.gene80732.t1
chr8.4	CDS	16297436	16297562	16297436	ID=cds.MS.gene80732.t1;Parent=MS.gene80732.t1
chr8.4	exon	16297742	16297851	16297742	ID=MS.gene80732.t1.exon2;Parent=MS.gene80732.t1
chr8.4	CDS	16297742	16297851	16297742	ID=cds.MS.gene80732.t1;Parent=MS.gene80732.t1

Gene Sequence

>MS.gene80732

ACAGCAAAAGGTGCAAAAAATATACAGCGCTGCTGTAGCTGCTACAGCTGAAGCTACGACAGGAAAAGAGGGAGAAGAAAAGGTCAAGCTACACACACACTCTTACTGCTGTCTAGATTATGCACAGGTCAATCTCTCCACTGATTGTATTCTTCAGCGTAGCTTTACCAATGATATTCAGTTGTTTTATTGCAGAGAGAAGGGAAAATCAATGTTATTTTGCCTGCTTTTCAGATAATTCAATTGAATTGGTACATCAAAATTTGGGCTCTTTCATAAATAGGATACTTGTGATTTTGTTGGCAGATATATGATGTGGTTAGGTACAGACTGCATCGCATGAAGCATAAGCTGAAAGATGAATTAGAGAACAAGAACACCATTCAGGAATATATATGTACAAACTGTGGCAAAAG

Protein sequence

>MS.gene80732.t1

TAKGAKNIQRCCSCYSUSYDRKRGRRKGQATHTLLLLSRLCTDIUCGUVQTASHEAUAERUIREQEHHSGIYMYKLWQK