AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56565


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56565.t1 MTR_4g082350 98.883 358 4 0 1 358 1 358 0.0 723
MS.gene56565.t1 MTR_4g109020 28.082 292 198 9 49 331 6 294 7.34e-29 114
MS.gene56565.t1 MTR_3g037110 27.703 296 186 8 49 330 6 287 6.45e-26 106
MS.gene56565.t1 MTR_4g096890 26.073 303 197 7 41 336 7 289 1.80e-17 82.4
MS.gene56565.t1 MTR_7g021620 26.316 304 199 7 39 336 5 289 8.51e-17 80.5
MS.gene56565.t1 MTR_3g088975 24.756 307 211 6 39 336 5 300 2.06e-15 76.6
MS.gene56565.t1 MTR_1g029440 23.127 307 225 6 35 336 1 301 1.05e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56565.t1 AT1G69430 66.462 325 102 2 17 335 8 331 2.83e-154 437
MS.gene56565.t1 AT1G26650 63.939 330 116 3 24 350 2 331 8.05e-154 436
MS.gene56565.t1 AT4G19950 27.451 306 190 9 41 336 7 290 8.72e-15 74.7
MS.gene56565.t1 AT5G61340 28.311 219 136 8 119 329 80 285 1.57e-12 68.2
MS.gene56565.t1 AT5G61340 28.311 219 136 8 119 329 113 318 1.59e-12 68.6

Find 67 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGGTTCTGGAGGATGTTTC+GGG 0.171105 4.2:+27420834 MS.gene56565:CDS
CTCGGTTCTGGAGGATGTTT+CGG 0.203669 4.2:+27420833 MS.gene56565:CDS
GAGAAACTATAAGGATTCTT+AGG 0.245365 4.2:+27420265 MS.gene56565:CDS
TGTAGTGCATTGGCTGTTCT+TGG 0.255096 4.2:+27420708 MS.gene56565:CDS
TTGTGAAGAATCTTACTATT+AGG 0.290531 4.2:+27420379 MS.gene56565:CDS
GAGGTTGTTCTCCTAGGATT+AGG 0.293873 4.2:+27420127 MS.gene56565:CDS
TGCGTTGGTGATCTCGGTTC+TGG 0.297916 4.2:+27420821 MS.gene56565:CDS
TATTCGAGAAAGAAGTTTGA+TGG 0.334246 4.2:+27420567 MS.gene56565:CDS
TTTACGTCAAGCTTTAGAAT+TGG 0.356836 4.2:+27420188 MS.gene56565:CDS
ATATTCTTAGAGAAACTATA+AGG 0.357854 4.2:+27420256 MS.gene56565:CDS
GCCCATTGTTTCAGCAGAAA+TGG 0.362209 4.2:-27421156 None:intergenic
AATTCAATCAATGAATGCTT+TGG 0.379030 4.2:+27420233 MS.gene56565:CDS
TGTGCTATTGTCTAATGTAT+TGG 0.379670 4.2:+27420344 MS.gene56565:CDS
ATATATCTTGGATCAACAAT+AGG 0.400163 4.2:+27420918 MS.gene56565:CDS
TGTTGTGTATGCTGCATTGA+TGG 0.401523 4.2:+27420746 MS.gene56565:CDS
AGGTTGTTCTCCTAGGATTA+GGG 0.404867 4.2:+27420128 MS.gene56565:CDS
CAGCGTTGCGTTGGTGATCT+CGG 0.412590 4.2:+27420815 MS.gene56565:CDS
GTGTGATCGTGGCAAAGTTT+TGG 0.412812 4.2:+27420601 MS.gene56565:CDS
GTCGGTCTGCTAATATATCT+TGG 0.415716 4.2:+27420906 MS.gene56565:CDS
GCAGCATACACAACAACATT+AGG 0.417117 4.2:-27420738 None:intergenic
GGCCATTTCTGCTGAAACAA+TGG 0.429412 4.2:+27421154 MS.gene56565:CDS
GACAATAGCACAGCAGAAAC+AGG 0.430186 4.2:-27420333 None:intergenic
AACAATAGGAATGGCCTTTG+TGG 0.433278 4.2:+27420932 MS.gene56565:CDS
TGCGGATACGGTGATTGCTT+CGG 0.443552 4.2:+27420470 MS.gene56565:CDS
TGGGCTTGTACAGTTATTGT+TGG 0.445242 4.2:+27420648 MS.gene56565:CDS
ATTGTTGTTATTGTCGAAAA+CGG 0.446504 4.2:+27420518 MS.gene56565:CDS
CAAGTGGAGGTTGTTCTCCT+AGG 0.449328 4.2:+27420121 MS.gene56565:CDS
GTAGTGCATTGGCTGTTCTT+GGG 0.473904 4.2:+27420709 MS.gene56565:CDS
GCCATTTCTGCTGAAACAAT+GGG 0.478130 4.2:+27421155 MS.gene56565:CDS
AGATACTTGCGACGTATATG+TGG 0.480298 4.2:+27420628 MS.gene56565:CDS
TTGTCAGAGATTTGCGGATA+CGG 0.482242 4.2:+27420458 MS.gene56565:CDS
TGACAAGATTGCTTAATCAT+AGG 0.488189 4.2:-27420441 None:intergenic
AATCATTTGCAGCGTTGCGT+TGG 0.493610 4.2:+27420806 MS.gene56565:CDS
GGATTCTTAGGTTTAATGCA+TGG 0.498954 4.2:+27420277 MS.gene56565:CDS
TCTTACTATTAGGCTTATGT+TGG 0.501948 4.2:+27420389 MS.gene56565:CDS
TTTAGCATGGAGAACTCAAA+TGG 0.507639 4.2:+27421104 MS.gene56565:CDS
GCAATCTTGTCAGAGATTTG+CGG 0.510716 4.2:+27420452 MS.gene56565:CDS
ATATGATCTACCCTAATCCT+AGG 0.527505 4.2:-27420138 None:intergenic
AATGTGGGAAGGGCCGCTCT+TGG 0.532814 4.2:+27421010 MS.gene56565:CDS
GATTCTTAGGTTTAATGCAT+GGG 0.536767 4.2:+27420278 MS.gene56565:CDS
ATAGGAATGGCCTTTGTGGA+GGG 0.546492 4.2:+27420936 MS.gene56565:CDS
TGCTTAATCATAGGTCTCAA+AGG 0.555452 4.2:-27420432 None:intergenic
GTTATTGTCGAAAACGGCTG+TGG 0.558964 4.2:+27420524 MS.gene56565:CDS
GTGTATGCTGCATTGATGGT+CGG 0.566529 4.2:+27420750 MS.gene56565:CDS
GTGATGGATCTTCGAGAATG+TGG 0.574992 4.2:+27420994 MS.gene56565:CDS
AATAGGAATGGCCTTTGTGG+AGG 0.586579 4.2:+27420935 MS.gene56565:CDS
TCTTGGATCAACAATAGGAA+TGG 0.587680 4.2:+27420923 MS.gene56565:CDS
TTTAATCAAAGGTCAGACTC+AGG 0.592291 4.2:+27420884 MS.gene56565:CDS
AGAGTAAAGACACTGAGTTA+TGG 0.598968 4.2:+27420972 MS.gene56565:CDS
TAGGAATGGCCTTTGTGGAG+GGG 0.600285 4.2:+27420937 MS.gene56565:CDS
ATCAAAGGTCAGACTCAGGT+CGG 0.608137 4.2:+27420888 MS.gene56565:CDS
CGGTTCTGGAGGATGTTTCG+GGG 0.610462 4.2:+27420835 MS.gene56565:CDS
AAAATGGAGATTTCAAGTGG+AGG 0.612604 4.2:+27420108 None:intergenic
ATGGAGAACTCAAATGGCGA+TGG 0.614239 4.2:+27421110 MS.gene56565:CDS
ATGTTGGTTGCTGAAACTAG+TGG 0.620640 4.2:+27420405 MS.gene56565:CDS
TATAGATTCTATGATGAGTG+CGG 0.621002 4.2:+27421058 MS.gene56565:CDS
ATGCACTACAAAACGCAACA+AGG 0.631744 4.2:-27420695 None:intergenic
GATACTTGCGACGTATATGT+GGG 0.634555 4.2:+27420629 MS.gene56565:CDS
AACAACAATGTAGCATACAA+AGG 0.636060 4.2:-27420504 None:intergenic
GTTGGTGATCTCGGTTCTGG+AGG 0.638193 4.2:+27420824 MS.gene56565:CDS
AAGACACTGAGTTATGGTGA+TGG 0.640856 4.2:+27420978 MS.gene56565:CDS
GATCTTCGAGAATGTGGGAA+GGG 0.641099 4.2:+27421000 MS.gene56565:CDS
GGATCTTCGAGAATGTGGGA+AGG 0.642671 4.2:+27420999 MS.gene56565:CDS
CTCAAACAACCCCTCCACAA+AGG 0.646189 4.2:-27420946 None:intergenic
GAATACATTATCACCAAGAG+CGG 0.651587 4.2:-27421023 None:intergenic
GGTTCTGGAGGATGTTTCGG+GGG 0.680880 4.2:+27420836 MS.gene56565:CDS
TGATGGATCTTCGAGAATGT+GGG 0.681312 4.2:+27420995 MS.gene56565:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTCTTAGAGAAACTATA+AGG + chr4.2:27420256-27420275 MS.gene56565:CDS 20.0%
! AATTCAATCAATGAATGCTT+TGG + chr4.2:27420233-27420252 MS.gene56565:CDS 25.0%
! ATATATCTTGGATCAACAAT+AGG + chr4.2:27420918-27420937 MS.gene56565:CDS 25.0%
! GTAGATCATATGAAAAAAGA+GGG + chr4.2:27420150-27420169 MS.gene56565:CDS 25.0%
! TTGTGAAGAATCTTACTATT+AGG + chr4.2:27420379-27420398 MS.gene56565:CDS 25.0%
!! ATTGTTGTTATTGTCGAAAA+CGG + chr4.2:27420518-27420537 MS.gene56565:CDS 25.0%
!!! CAACTCAATTTTAGAAACTA+TGG + chr4.2:27421133-27421152 MS.gene56565:CDS 25.0%
AACAACAATGTAGCATACAA+AGG - chr4.2:27420507-27420526 None:intergenic 30.0%
GAGAAACTATAAGGATTCTT+AGG + chr4.2:27420265-27420284 MS.gene56565:CDS 30.0%
GATTCTTAGGTTTAATGCAT+GGG + chr4.2:27420278-27420297 MS.gene56565:CDS 30.0%
GGTAGATCATATGAAAAAAG+AGG + chr4.2:27420149-27420168 MS.gene56565:CDS 30.0%
TATAGATTCTATGATGAGTG+CGG + chr4.2:27421058-27421077 MS.gene56565:CDS 30.0%
TATTCGAGAAAGAAGTTTGA+TGG + chr4.2:27420567-27420586 MS.gene56565:CDS 30.0%
TCTTACTATTAGGCTTATGT+TGG + chr4.2:27420389-27420408 MS.gene56565:CDS 30.0%
TGACAAGATTGCTTAATCAT+AGG - chr4.2:27420444-27420463 None:intergenic 30.0%
! TGTGCTATTGTCTAATGTAT+TGG + chr4.2:27420344-27420363 MS.gene56565:CDS 30.0%
!! GGTGATAATGTATTCTTTTG+TGG + chr4.2:27421031-27421050 MS.gene56565:CDS 30.0%
!! TTTACGTCAAGCTTTAGAAT+TGG + chr4.2:27420188-27420207 MS.gene56565:CDS 30.0%
!!! AGGTCGAGTATTTTAATCAA+AGG + chr4.2:27420873-27420892 MS.gene56565:CDS 30.0%
AGAGTAAAGACACTGAGTTA+TGG + chr4.2:27420972-27420991 MS.gene56565:CDS 35.0%
ATATGATCTACCCTAATCCT+AGG - chr4.2:27420141-27420160 None:intergenic 35.0%
GAATACATTATCACCAAGAG+CGG - chr4.2:27421026-27421045 None:intergenic 35.0%
GGATTCTTAGGTTTAATGCA+TGG + chr4.2:27420277-27420296 MS.gene56565:CDS 35.0%
TCTTGGATCAACAATAGGAA+TGG + chr4.2:27420923-27420942 MS.gene56565:CDS 35.0%
TGCTTAATCATAGGTCTCAA+AGG - chr4.2:27420435-27420454 None:intergenic 35.0%
TTTAATCAAAGGTCAGACTC+AGG + chr4.2:27420884-27420903 MS.gene56565:CDS 35.0%
TTTAGCATGGAGAACTCAAA+TGG + chr4.2:27421104-27421123 MS.gene56565:CDS 35.0%
! CAGTTGTAGATCTTTTAGCA+TGG + chr4.2:27421091-27421110 MS.gene56565:CDS 35.0%
AACAATAGGAATGGCCTTTG+TGG + chr4.2:27420932-27420951 MS.gene56565:CDS 40.0%
AAGACACTGAGTTATGGTGA+TGG + chr4.2:27420978-27420997 MS.gene56565:CDS 40.0%
AGATACTTGCGACGTATATG+TGG + chr4.2:27420628-27420647 MS.gene56565:CDS 40.0%
AGGTTGTTCTCCTAGGATTA+GGG + chr4.2:27420128-27420147 MS.gene56565:CDS 40.0%
ATGCACTACAAAACGCAACA+AGG - chr4.2:27420698-27420717 None:intergenic 40.0%
GATACTTGCGACGTATATGT+GGG + chr4.2:27420629-27420648 MS.gene56565:CDS 40.0%
GCAATCTTGTCAGAGATTTG+CGG + chr4.2:27420452-27420471 MS.gene56565:CDS 40.0%
GCAGCATACACAACAACATT+AGG - chr4.2:27420741-27420760 None:intergenic 40.0%
GCCATTTCTGCTGAAACAAT+GGG + chr4.2:27421155-27421174 MS.gene56565:CDS 40.0%
GTCGGTCTGCTAATATATCT+TGG + chr4.2:27420906-27420925 MS.gene56565:CDS 40.0%
TGGGCTTGTACAGTTATTGT+TGG + chr4.2:27420648-27420667 MS.gene56565:CDS 40.0%
TGTTGTGTATGCTGCATTGA+TGG + chr4.2:27420746-27420765 MS.gene56565:CDS 40.0%
TTGTCAGAGATTTGCGGATA+CGG + chr4.2:27420458-27420477 MS.gene56565:CDS 40.0%
! ATGTTGGTTGCTGAAACTAG+TGG + chr4.2:27420405-27420424 MS.gene56565:CDS 40.0%
! TGATGGATCTTCGAGAATGT+GGG + chr4.2:27420995-27421014 MS.gene56565:CDS 40.0%
! TGTTGCGTTTTGTAGTGCAT+TGG + chr4.2:27420698-27420717 MS.gene56565:CDS 40.0%
!! TTCGAAGTTTTGTGTGATCG+TGG + chr4.2:27420590-27420609 MS.gene56565:CDS 40.0%
AATAGGAATGGCCTTTGTGG+AGG + chr4.2:27420935-27420954 MS.gene56565:CDS 45.0%
AATCATTTGCAGCGTTGCGT+TGG + chr4.2:27420806-27420825 MS.gene56565:CDS 45.0%
ATAGGAATGGCCTTTGTGGA+GGG + chr4.2:27420936-27420955 MS.gene56565:CDS 45.0%
ATCAAAGGTCAGACTCAGGT+CGG + chr4.2:27420888-27420907 MS.gene56565:CDS 45.0%
ATGGAGAACTCAAATGGCGA+TGG + chr4.2:27421110-27421129 MS.gene56565:CDS 45.0%
GACAATAGCACAGCAGAAAC+AGG - chr4.2:27420336-27420355 None:intergenic 45.0%
GAGGTTGTTCTCCTAGGATT+AGG + chr4.2:27420127-27420146 MS.gene56565:CDS 45.0%
GATCTTCGAGAATGTGGGAA+GGG + chr4.2:27421000-27421019 MS.gene56565:CDS 45.0%
GCCCATTGTTTCAGCAGAAA+TGG - chr4.2:27421159-27421178 None:intergenic 45.0%
GGCCATTTCTGCTGAAACAA+TGG + chr4.2:27421154-27421173 MS.gene56565:CDS 45.0%
GTTATTGTCGAAAACGGCTG+TGG + chr4.2:27420524-27420543 MS.gene56565:CDS 45.0%
! GTGTATGCTGCATTGATGGT+CGG + chr4.2:27420750-27420769 MS.gene56565:CDS 45.0%
! GTGTGATCGTGGCAAAGTTT+TGG + chr4.2:27420601-27420620 MS.gene56565:CDS 45.0%
!! GTAGTGCATTGGCTGTTCTT+GGG + chr4.2:27420709-27420728 MS.gene56565:CDS 45.0%
!! GTGATGGATCTTCGAGAATG+TGG + chr4.2:27420994-27421013 MS.gene56565:CDS 45.0%
!! TGATCGTGGCAAAGTTTTGG+AGG + chr4.2:27420604-27420623 MS.gene56565:CDS 45.0%
!! TGTAGTGCATTGGCTGTTCT+TGG + chr4.2:27420708-27420727 MS.gene56565:CDS 45.0%
CAAGTGGAGGTTGTTCTCCT+AGG + chr4.2:27420121-27420140 MS.gene56565:CDS 50.0%
CTCAAACAACCCCTCCACAA+AGG - chr4.2:27420949-27420968 None:intergenic 50.0%
GGATCTTCGAGAATGTGGGA+AGG + chr4.2:27420999-27421018 MS.gene56565:CDS 50.0%
TAGGAATGGCCTTTGTGGAG+GGG + chr4.2:27420937-27420956 MS.gene56565:CDS 50.0%
TGCGGATACGGTGATTGCTT+CGG + chr4.2:27420470-27420489 MS.gene56565:CDS 50.0%
!! CTCGGTTCTGGAGGATGTTT+CGG + chr4.2:27420833-27420852 MS.gene56565:CDS 50.0%
!! TCGGTTCTGGAGGATGTTTC+GGG + chr4.2:27420834-27420853 MS.gene56565:CDS 50.0%
!! CAGCGTTGCGTTGGTGATCT+CGG + chr4.2:27420815-27420834 MS.gene56565:CDS 55.0%
!! CGGTTCTGGAGGATGTTTCG+GGG + chr4.2:27420835-27420854 MS.gene56565:CDS 55.0%
!! GGTTCTGGAGGATGTTTCGG+GGG + chr4.2:27420836-27420855 MS.gene56565:CDS 55.0%
!! GTTGGTGATCTCGGTTCTGG+AGG + chr4.2:27420824-27420843 MS.gene56565:CDS 55.0%
!! TGCGTTGGTGATCTCGGTTC+TGG + chr4.2:27420821-27420840 MS.gene56565:CDS 55.0%
AATGTGGGAAGGGCCGCTCT+TGG + chr4.2:27421010-27421029 MS.gene56565:CDS 60.0%
! GGAGGATGTTTCGGGGGCGC+AGG + chr4.2:27420842-27420861 MS.gene56565:CDS 70.0%
! CGGGGGCGCAGGCGATGCTA+AGG + chr4.2:27420853-27420872 MS.gene56565:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 27420111 27421187 27420111 ID=MS.gene56565
chr4.2 mRNA 27420111 27421187 27420111 ID=MS.gene56565.t1;Parent=MS.gene56565
chr4.2 exon 27420111 27421187 27420111 ID=MS.gene56565.t1.exon1;Parent=MS.gene56565.t1
chr4.2 CDS 27420111 27421187 27420111 ID=cds.MS.gene56565.t1;Parent=MS.gene56565.t1
Gene Sequence

>MS.gene56565

ATGGAGATTTCAAGTGGAGGTTGTTCTCCTAGGATTAGGGTAGATCATATGAAAAAAGAGGGATCAGATGATACACGTTTACGTCAAGCTTTAGAATTGGAACTTTCTGATCATAATCATGAAATTCAATCAATGAATGCTTTGGATATTCTTAGAGAAACTATAAGGATTCTTAGGTTTAATGCATGGGCTTTTATGATAATCGCTTTTTTGCTTATTTGTCCTGTTTCTGCTGTGCTATTGTCTAATGTATTGGTTGATGAATCTATTGTGAAGAATCTTACTATTAGGCTTATGTTGGTTGCTGAAACTAGTGGTCTTCCTTTGAGACCTATGATTAAGCAATCTTGTCAGAGATTTGCGGATACGGTGATTGCTTCGGCGATGTGTTTTCCTTTGTATGCTACATTGTTGTTATTGTCGAAAACGGCTGTGGTTTATTCTGTTGATTGTACTTATTCGAGAAAGAAGTTTGATGGTTCGAAGTTTTGTGTGATCGTGGCAAAGTTTTGGAGGAAGATACTTGCGACGTATATGTGGGCTTGTACAGTTATTGTTGGTTGCATCACTATGTTTTGTGTTTTCCTTGTTGCGTTTTGTAGTGCATTGGCTGTTCTTGGGTTTTCTCCTAATGTTGTTGTGTATGCTGCATTGATGGTCGGATTAGTTTTCTCTGTTGTATTTGCGAATGCGATAATCATTTGCAGCGTTGCGTTGGTGATCTCGGTTCTGGAGGATGTTTCGGGGGCGCAGGCGATGCTAAGGTCGAGTATTTTAATCAAAGGTCAGACTCAGGTCGGTCTGCTAATATATCTTGGATCAACAATAGGAATGGCCTTTGTGGAGGGGTTGTTTGAGCACAGAGTAAAGACACTGAGTTATGGTGATGGATCTTCGAGAATGTGGGAAGGGCCGCTCTTGGTGATAATGTATTCTTTTGTGGTGCTTATAGATTCTATGATGAGTGCGGTTTTCTATTTCAGTTGTAGATCTTTTAGCATGGAGAACTCAAATGGCGATGGCAACTCAATTTTAGAAACTATGGCCATTTCTGCTGAAACAATGGGCATTCAATGA

Protein sequence

>MS.gene56565.t1

MEISSGGCSPRIRVDHMKKEGSDDTRLRQALELELSDHNHEIQSMNALDILRETIRILRFNAWAFMIIAFLLICPVSAVLLSNVLVDESIVKNLTIRLMLVAETSGLPLRPMIKQSCQRFADTVIASAMCFPLYATLLLLSKTAVVYSVDCTYSRKKFDGSKFCVIVAKFWRKILATYMWACTVIVGCITMFCVFLVAFCSALAVLGFSPNVVVYAALMVGLVFSVVFANAIIICSVALVISVLEDVSGAQAMLRSSILIKGQTQVGLLIYLGSTIGMAFVEGLFEHRVKTLSYGDGSSRMWEGPLLVIMYSFVVLIDSMMSAVFYFSCRSFSMENSNGDGNSILETMAISAETMGIQ