Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29844.t1 | XP_013460856.1 | 94.2 | 326 | 18 | 1 | 1 | 326 | 1 | 325 | 9.90E-171 | 609.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29844.t1 | Q55CU8 | 25.6 | 246 | 175 | 4 | 20 | 257 | 43 | 288 | 6.6e-22 | 106.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29844.t1 | G8A376 | 94.2 | 326 | 18 | 1 | 1 | 326 | 1 | 325 | 7.2e-171 | 609.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene29844 | 0.841167 | 5.60E-58 | -1.69E-46 |
| MS.gene049153 | MS.gene29844 | 0.846563 | 2.01E-59 | -1.69E-46 |
| MS.gene049260 | MS.gene29844 | 0.8039 | 2.79E-49 | -1.69E-46 |
| MS.gene049356 | MS.gene29844 | 0.885792 | 5.99E-72 | -1.69E-46 |
| MS.gene049369 | MS.gene29844 | 0.842737 | 2.15E-58 | -1.69E-46 |
| MS.gene049661 | MS.gene29844 | 0.811313 | 7.45E-51 | -1.69E-46 |
| MS.gene049662 | MS.gene29844 | 0.802151 | 6.43E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene29844 | 0.818854 | 1.58E-52 | -1.69E-46 |
| MS.gene050128 | MS.gene29844 | 0.851184 | 1.05E-60 | -1.69E-46 |
| MS.gene050301 | MS.gene29844 | 0.835461 | 1.66E-56 | -1.69E-46 |
| MS.gene050397 | MS.gene29844 | 0.806975 | 6.33E-50 | -1.69E-46 |
| MS.gene050432 | MS.gene29844 | 0.824644 | 7.22E-54 | -1.69E-46 |
| MS.gene050670 | MS.gene29844 | 0.819012 | 1.45E-52 | -1.69E-46 |
| MS.gene050971 | MS.gene29844 | 0.809878 | 1.52E-50 | -1.69E-46 |
| MS.gene05105 | MS.gene29844 | 0.80694 | 6.44E-50 | -1.69E-46 |
| MS.gene051360 | MS.gene29844 | 0.815186 | 1.05E-51 | -1.69E-46 |
| MS.gene051860 | MS.gene29844 | 0.823368 | 1.44E-53 | -1.69E-46 |
| MS.gene052077 | MS.gene29844 | 0.805026 | 1.63E-49 | -1.69E-46 |
| MS.gene052178 | MS.gene29844 | 0.811577 | 6.53E-51 | -1.69E-46 |
| MS.gene052188 | MS.gene29844 | 0.860634 | 1.80E-63 | -1.69E-46 |
| MS.gene052253 | MS.gene29844 | 0.859004 | 5.58E-63 | -1.69E-46 |
| MS.gene052255 | MS.gene29844 | 0.805423 | 1.34E-49 | -1.69E-46 |
| MS.gene052310 | MS.gene29844 | 0.800009 | 1.76E-48 | -1.69E-46 |
| MS.gene052340 | MS.gene29844 | 0.861891 | 7.46E-64 | -1.69E-46 |
| MS.gene052485 | MS.gene29844 | 0.828416 | 9.10E-55 | -1.69E-46 |
| MS.gene05316 | MS.gene29844 | 0.859866 | 3.08E-63 | -1.69E-46 |
| MS.gene053209 | MS.gene29844 | 0.831766 | 1.39E-55 | -1.69E-46 |
| MS.gene053449 | MS.gene29844 | 0.806697 | 7.25E-50 | -1.69E-46 |
| MS.gene053557 | MS.gene29844 | 0.812665 | 3.78E-51 | -1.69E-46 |
| MS.gene053758 | MS.gene29844 | 0.819706 | 1.01E-52 | -1.69E-46 |
| MS.gene053813 | MS.gene29844 | 0.815339 | 9.72E-52 | -1.69E-46 |
| MS.gene053836 | MS.gene29844 | 0.810314 | 1.23E-50 | -1.69E-46 |
| MS.gene053912 | MS.gene29844 | 0.809073 | 2.27E-50 | -1.69E-46 |
| MS.gene053928 | MS.gene29844 | 0.801691 | 7.99E-49 | -1.69E-46 |
| MS.gene054010 | MS.gene29844 | 0.824224 | 9.06E-54 | -1.69E-46 |
| MS.gene054011 | MS.gene29844 | 0.822625 | 2.14E-53 | -1.69E-46 |
| MS.gene054012 | MS.gene29844 | 0.823039 | 1.72E-53 | -1.69E-46 |
| MS.gene054013 | MS.gene29844 | 0.816367 | 5.74E-52 | -1.69E-46 |
| MS.gene054123 | MS.gene29844 | 0.801501 | 8.74E-49 | -1.69E-46 |
| MS.gene054183 | MS.gene29844 | 0.830129 | 3.49E-55 | -1.69E-46 |
| MS.gene054349 | MS.gene29844 | 0.831813 | 1.35E-55 | -1.69E-46 |
| MS.gene055116 | MS.gene29844 | 0.852358 | 4.87E-61 | -1.69E-46 |
| MS.gene055277 | MS.gene29844 | 0.837738 | 4.35E-57 | -1.69E-46 |
| MS.gene055390 | MS.gene29844 | 0.80527 | 1.45E-49 | -1.69E-46 |
| MS.gene055422 | MS.gene29844 | 0.816757 | 4.69E-52 | -1.69E-46 |
| MS.gene055604 | MS.gene29844 | 0.84622 | 2.49E-59 | -1.69E-46 |
| MS.gene055666 | MS.gene29844 | 0.822589 | 2.18E-53 | -1.69E-46 |
| MS.gene056191 | MS.gene29844 | 0.821407 | 4.11E-53 | -1.69E-46 |
| MS.gene056422 | MS.gene29844 | 0.815016 | 1.15E-51 | -1.69E-46 |
| MS.gene056511 | MS.gene29844 | 0.823461 | 1.37E-53 | -1.69E-46 |
| MS.gene056609 | MS.gene29844 | 0.813781 | 2.15E-51 | -1.69E-46 |
| MS.gene056659 | MS.gene29844 | 0.823122 | 1.64E-53 | -1.69E-46 |
| MS.gene056723 | MS.gene29844 | 0.81042 | 1.16E-50 | -1.69E-46 |
| MS.gene056902 | MS.gene29844 | 0.807874 | 4.08E-50 | -1.69E-46 |
| MS.gene05702 | MS.gene29844 | 0.831791 | 1.37E-55 | -1.69E-46 |
| MS.gene057124 | MS.gene29844 | 0.844698 | 6.43E-59 | -1.69E-46 |
| MS.gene05735 | MS.gene29844 | 0.815122 | 1.09E-51 | -1.69E-46 |
| MS.gene057396 | MS.gene29844 | 0.848329 | 6.57E-60 | -1.69E-46 |
| MS.gene057448 | MS.gene29844 | 0.856351 | 3.41E-62 | -1.69E-46 |
| MS.gene057449 | MS.gene29844 | 0.859417 | 4.20E-63 | -1.69E-46 |
| MS.gene057450 | MS.gene29844 | 0.854021 | 1.62E-61 | -1.69E-46 |
| MS.gene057469 | MS.gene29844 | 0.810849 | 9.39E-51 | -1.69E-46 |
| MS.gene057537 | MS.gene29844 | 0.844134 | 9.13E-59 | -1.69E-46 |
| MS.gene057749 | MS.gene29844 | 0.832251 | 1.05E-55 | -1.69E-46 |
| MS.gene057796 | MS.gene29844 | 0.806187 | 9.29E-50 | -1.69E-46 |
| MS.gene057823 | MS.gene29844 | 0.825597 | 4.30E-54 | -1.69E-46 |
| MS.gene058002 | MS.gene29844 | 0.830106 | 3.54E-55 | -1.69E-46 |
| MS.gene05811 | MS.gene29844 | 0.823107 | 1.65E-53 | -1.69E-46 |
| MS.gene058365 | MS.gene29844 | 0.818951 | 1.50E-52 | -1.69E-46 |
| MS.gene05842 | MS.gene29844 | 0.830574 | 2.72E-55 | -1.69E-46 |
| MS.gene05858 | MS.gene29844 | 0.805043 | 1.61E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene29844 | 0.807537 | 4.82E-50 | -1.69E-46 |
| MS.gene058710 | MS.gene29844 | 0.803549 | 3.31E-49 | -1.69E-46 |
| MS.gene05886 | MS.gene29844 | 0.825274 | 5.13E-54 | -1.69E-46 |
| MS.gene058928 | MS.gene29844 | 0.875242 | 3.60E-68 | -1.69E-46 |
| MS.gene058949 | MS.gene29844 | 0.855602 | 5.65E-62 | -1.69E-46 |
| MS.gene059011 | MS.gene29844 | 0.814342 | 1.62E-51 | -1.69E-46 |
| MS.gene05907 | MS.gene29844 | 0.822477 | 2.32E-53 | -1.69E-46 |
| MS.gene059285 | MS.gene29844 | 0.847601 | 1.04E-59 | -1.69E-46 |
| MS.gene059294 | MS.gene29844 | 0.837003 | 6.72E-57 | -1.69E-46 |
| MS.gene059366 | MS.gene29844 | 0.853768 | 1.92E-61 | -1.69E-46 |
| MS.gene059420 | MS.gene29844 | 0.85527 | 7.06E-62 | -1.69E-46 |
| MS.gene059462 | MS.gene29844 | 0.810631 | 1.05E-50 | -1.69E-46 |
| MS.gene059597 | MS.gene29844 | 0.813275 | 2.78E-51 | -1.69E-46 |
| MS.gene059674 | MS.gene29844 | 0.825632 | 4.22E-54 | -1.69E-46 |
| MS.gene059688 | MS.gene29844 | 0.843893 | 1.06E-58 | -1.69E-46 |
| MS.gene059692 | MS.gene29844 | 0.83776 | 4.30E-57 | -1.69E-46 |
| MS.gene059695 | MS.gene29844 | 0.833868 | 4.16E-56 | -1.69E-46 |
| MS.gene059711 | MS.gene29844 | 0.835078 | 2.07E-56 | -1.69E-46 |
| MS.gene059807 | MS.gene29844 | 0.859176 | 4.96E-63 | -1.69E-46 |
| MS.gene05984 | MS.gene29844 | 0.823071 | 1.69E-53 | -1.69E-46 |
| MS.gene059876 | MS.gene29844 | 0.811201 | 7.88E-51 | -1.69E-46 |
| MS.gene059894 | MS.gene29844 | 0.819457 | 1.15E-52 | -1.69E-46 |
| MS.gene059974 | MS.gene29844 | 0.824731 | 6.88E-54 | -1.69E-46 |
| MS.gene060247 | MS.gene29844 | 0.809941 | 1.47E-50 | -1.69E-46 |
| MS.gene06037 | MS.gene29844 | 0.849789 | 2.58E-60 | -1.69E-46 |
| MS.gene060495 | MS.gene29844 | 0.851411 | 9.03E-61 | -1.69E-46 |
| MS.gene060837 | MS.gene29844 | 0.834092 | 3.66E-56 | -1.69E-46 |
| MS.gene061049 | MS.gene29844 | 0.854566 | 1.13E-61 | -1.69E-46 |
| MS.gene061051 | MS.gene29844 | -0.841788 | 3.84E-58 | -1.69E-46 |
| MS.gene061159 | MS.gene29844 | 0.806877 | 6.64E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29844.t1 | MTR_3g074050 | 94.172 | 326 | 18 | 1 | 1 | 326 | 1 | 325 | 0.0 | 640 |
| MS.gene29844.t1 | MTR_3g074050 | 93.865 | 326 | 18 | 2 | 1 | 326 | 1 | 324 | 0.0 | 634 |
| MS.gene29844.t1 | MTR_3g074050 | 93.023 | 258 | 17 | 1 | 69 | 326 | 1 | 257 | 0.0 | 506 |
| MS.gene29844.t1 | MTR_5g062310 | 65.147 | 307 | 104 | 1 | 1 | 307 | 1 | 304 | 4.22e-149 | 422 |
| MS.gene29844.t1 | MTR_5g062310 | 64.435 | 239 | 85 | 0 | 69 | 307 | 1 | 239 | 1.57e-115 | 335 |
| MS.gene29844.t1 | MTR_7g087500 | 43.416 | 281 | 155 | 1 | 23 | 303 | 18 | 294 | 3.56e-90 | 273 |
| MS.gene29844.t1 | MTR_8g077700 | 47.638 | 254 | 132 | 1 | 18 | 270 | 12 | 265 | 1.12e-84 | 257 |
| MS.gene29844.t1 | MTR_8g077700 | 47.638 | 254 | 132 | 1 | 18 | 270 | 40 | 293 | 9.26e-84 | 256 |
| MS.gene29844.t1 | MTR_1g022185 | 43.902 | 246 | 137 | 1 | 26 | 270 | 20 | 265 | 5.67e-77 | 238 |
| MS.gene29844.t1 | MTR_8g096180 | 30.263 | 228 | 152 | 7 | 20 | 244 | 19 | 242 | 1.17e-23 | 97.8 |
| MS.gene29844.t1 | MTR_4g105450 | 27.219 | 169 | 120 | 2 | 52 | 218 | 72 | 239 | 1.43e-22 | 95.1 |
| MS.gene29844.t1 | MTR_4g105460 | 28.025 | 157 | 111 | 1 | 55 | 209 | 77 | 233 | 2.90e-21 | 91.7 |
| MS.gene29844.t1 | MTR_8g096170 | 29.515 | 227 | 153 | 7 | 21 | 244 | 23 | 245 | 8.49e-21 | 90.1 |
| MS.gene29844.t1 | MTR_8g096180 | 28.992 | 238 | 152 | 8 | 20 | 244 | 19 | 252 | 2.05e-20 | 89.4 |
| MS.gene29844.t1 | MTR_3g098530 | 26.106 | 226 | 160 | 7 | 22 | 244 | 41 | 262 | 3.49e-19 | 86.3 |
| MS.gene29844.t1 | MTR_1g104540 | 28.358 | 201 | 137 | 3 | 21 | 215 | 8 | 207 | 1.57e-18 | 85.9 |
| MS.gene29844.t1 | MTR_4g105460 | 28.358 | 134 | 90 | 3 | 55 | 185 | 77 | 207 | 4.48e-13 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29844.t1 | AT4G08690 | 52.529 | 257 | 119 | 1 | 9 | 265 | 6 | 259 | 3.92e-104 | 307 |
| MS.gene29844.t1 | AT4G08690 | 52.529 | 257 | 119 | 1 | 9 | 265 | 6 | 259 | 3.92e-104 | 307 |
| MS.gene29844.t1 | AT4G08690 | 52.529 | 257 | 119 | 1 | 9 | 265 | 6 | 259 | 3.92e-104 | 307 |
| MS.gene29844.t1 | AT1G22180 | 51.546 | 291 | 126 | 5 | 12 | 301 | 12 | 288 | 7.12e-102 | 302 |
| MS.gene29844.t1 | AT1G22180 | 51.546 | 291 | 126 | 5 | 12 | 301 | 12 | 288 | 7.12e-102 | 302 |
| MS.gene29844.t1 | AT1G22180 | 52.137 | 234 | 100 | 4 | 69 | 301 | 1 | 223 | 1.09e-83 | 253 |
| MS.gene29844.t1 | AT1G22180 | 52.137 | 234 | 100 | 4 | 69 | 301 | 1 | 223 | 1.09e-83 | 253 |
| MS.gene29844.t1 | AT1G75170 | 42.636 | 258 | 147 | 1 | 25 | 281 | 20 | 277 | 5.41e-79 | 243 |
| MS.gene29844.t1 | AT1G75170 | 42.636 | 258 | 147 | 1 | 25 | 281 | 20 | 277 | 5.41e-79 | 243 |
| MS.gene29844.t1 | AT4G36640 | 41.199 | 267 | 156 | 1 | 24 | 289 | 16 | 282 | 1.42e-75 | 234 |
| MS.gene29844.t1 | AT4G36640 | 41.199 | 267 | 156 | 1 | 24 | 289 | 16 | 282 | 1.42e-75 | 234 |
| MS.gene29844.t1 | AT4G36640 | 40.541 | 222 | 131 | 1 | 69 | 289 | 1 | 222 | 3.68e-59 | 190 |
| MS.gene29844.t1 | AT1G75170 | 38.860 | 193 | 117 | 1 | 90 | 281 | 2 | 194 | 6.52e-48 | 160 |
| MS.gene29844.t1 | AT5G63060 | 30.128 | 156 | 107 | 1 | 55 | 208 | 78 | 233 | 3.06e-21 | 91.7 |
| MS.gene29844.t1 | AT5G63060 | 30.189 | 159 | 109 | 1 | 52 | 208 | 40 | 198 | 3.96e-21 | 90.5 |
| MS.gene29844.t1 | AT1G01630 | 27.755 | 245 | 170 | 7 | 3 | 244 | 5 | 245 | 3.94e-20 | 88.6 |
| MS.gene29844.t1 | AT1G05370 | 26.816 | 179 | 128 | 2 | 39 | 215 | 32 | 209 | 1.10e-14 | 74.7 |
| MS.gene29844.t1 | AT3G22410 | 26.667 | 195 | 139 | 3 | 24 | 215 | 6 | 199 | 5.33e-13 | 69.7 |
Find 69 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATATTCCTGTCTCCATTTA+AGG | 0.158580 | 3.4:+72649437 | None:intergenic |
| GGTATTTAAAATCACAAAAT+TGG | 0.173573 | 3.4:-72649495 | MS.gene29844:CDS |
| CCATCCTTGGCACAATAATT+TGG | 0.174530 | 3.4:+72649121 | None:intergenic |
| CCATAATCTTCTTTGTGTTT+TGG | 0.230567 | 3.4:+72648485 | None:intergenic |
| TATGGAGGGTTTATTTGATA+TGG | 0.234312 | 3.4:-72648467 | MS.gene29844:CDS |
| ATAACAAGATGCACTCCTTT+TGG | 0.282527 | 3.4:-72648363 | MS.gene29844:CDS |
| CTTTATCTTATTATAATTCT+CGG | 0.306203 | 3.4:+72648525 | None:intergenic |
| CTTACCCAGGTAATCTCTTC+AGG | 0.306944 | 3.4:+72649412 | None:intergenic |
| CCAAAACACAAAGAAGATTA+TGG | 0.309315 | 3.4:-72648485 | MS.gene29844:CDS |
| GAGATATATTGATCAGGTTT+AGG | 0.320441 | 3.4:+72648226 | None:intergenic |
| CTCACCAGGAGCAGGTGATC+TGG | 0.345435 | 3.4:-72648878 | MS.gene29844:CDS |
| TTTGTTCTCATTATAAGTAC+TGG | 0.403459 | 3.4:+72649094 | None:intergenic |
| GTTCTGATGCATCCATTTCA+AGG | 0.412879 | 3.4:-72649516 | MS.gene29844:CDS |
| CAGAGACAGGGAAAATGTTT+AGG | 0.428866 | 3.4:-72649144 | MS.gene29844:CDS |
| ATCTGGCTGGTTGATTTCCA+TGG | 0.440350 | 3.4:-72648861 | MS.gene29844:CDS |
| GATCAACTTGAACCTGCATT+TGG | 0.447521 | 3.4:-72648445 | MS.gene29844:CDS |
| AAGTACTGGCCTTCCATCCT+TGG | 0.462466 | 3.4:+72649108 | None:intergenic |
| AGATCACCTGCTCCTGGTGA+GGG | 0.468118 | 3.4:+72648880 | None:intergenic |
| CAGATCACCTGCTCCTGGTG+AGG | 0.469356 | 3.4:+72648879 | None:intergenic |
| GTTGCTAATGAAGCAGAGAC+AGG | 0.477872 | 3.4:-72649157 | MS.gene29844:CDS |
| TATTTCGTTACCGAGAAGAA+TGG | 0.481590 | 3.4:+72648720 | None:intergenic |
| ATTGAGGTGAGAAAGTTGAT+AGG | 0.483915 | 3.4:-72649568 | MS.gene29844:CDS |
| CCAAATTATTGTGCCAAGGA+TGG | 0.499730 | 3.4:-72649121 | MS.gene29844:CDS |
| GTAACTGAGATATATTGATC+AGG | 0.500083 | 3.4:+72648220 | None:intergenic |
| CCAGGAGCAGGTGATCTGGC+TGG | 0.500834 | 3.4:-72648874 | MS.gene29844:CDS |
| TCATTATTCCCACCAAATGC+AGG | 0.506468 | 3.4:+72648433 | None:intergenic |
| CAACTTGAACCTGCATTTGG+TGG | 0.510649 | 3.4:-72648442 | MS.gene29844:CDS |
| GTCACTATCACAAGAACAGC+AGG | 0.515426 | 3.4:-72649702 | MS.gene29844:CDS |
| AGAGTTTGCATGTGTCCAAA+AGG | 0.518082 | 3.4:+72648348 | None:intergenic |
| CTGAAGAAAACTGCCTCTGA+TGG | 0.531528 | 3.4:-72649746 | MS.gene29844:CDS |
| TTCCTCCTTCAGATTTGACC+AGG | 0.531700 | 3.4:-72648303 | MS.gene29844:CDS |
| CACTTGACAAACTGAAACCA+TGG | 0.532083 | 3.4:+72648844 | None:intergenic |
| CCAGCCAGATCACCTGCTCC+TGG | 0.532714 | 3.4:+72648874 | None:intergenic |
| ATAAACCTGAAGAGATTACC+TGG | 0.535260 | 3.4:-72649417 | MS.gene29844:CDS |
| GAAGAATGGTTGGAAAATCC+CGG | 0.535429 | 3.4:+72648734 | None:intergenic |
| TTATCCACAGCGCCTCGGAT+TGG | 0.536069 | 3.4:-72648775 | MS.gene29844:CDS |
| GATAGGAACATTATCAGATA+AGG | 0.538137 | 3.4:-72649551 | MS.gene29844:CDS |
| ATCACCTGCTCCTGGTGAGG+GGG | 0.548989 | 3.4:+72648882 | None:intergenic |
| ATATGCTAAGAGAATGAGAG+AGG | 0.549993 | 3.4:-72648389 | MS.gene29844:CDS |
| AACTTGAACCTGCATTTGGT+GGG | 0.550189 | 3.4:-72648441 | MS.gene29844:CDS |
| TATAATGAGAACAAATCGCC+AGG | 0.555150 | 3.4:-72649086 | MS.gene29844:intron |
| AATATATCTCAGTTACTCGC+GGG | 0.560385 | 3.4:-72648213 | MS.gene29844:CDS |
| ATCAGAATCTGCTACTAACC+TGG | 0.564891 | 3.4:+72648285 | None:intergenic |
| TAGGCCAAATTATTGTGCCA+AGG | 0.566490 | 3.4:-72649125 | MS.gene29844:CDS |
| TGTTGCATTCCAGATCATTG+AGG | 0.566777 | 3.4:-72649584 | MS.gene29844:intron |
| ATTATTGTGCCAAGGATGGA+AGG | 0.571441 | 3.4:-72649117 | MS.gene29844:CDS |
| GATCACCTGCTCCTGGTGAG+GGG | 0.576919 | 3.4:+72648881 | None:intergenic |
| GCAATCATGTATGATGCACC+CGG | 0.576986 | 3.4:-72648753 | MS.gene29844:CDS |
| AAATATTATCCACAGCGCCT+CGG | 0.585906 | 3.4:-72648780 | MS.gene29844:CDS |
| TCGTTACCGAGAAGAATGGT+TGG | 0.590066 | 3.4:+72648724 | None:intergenic |
| AACACAAAGAAGATTATGGA+GGG | 0.591841 | 3.4:-72648481 | MS.gene29844:CDS |
| ATCACAAGAACAGCAGGCTA+AGG | 0.594560 | 3.4:-72649696 | MS.gene29844:intron |
| ACATTGTGTGAGACTGATGA+TGG | 0.595579 | 3.4:+72648325 | None:intergenic |
| AAGAATGGTTGGAAAATCCC+GGG | 0.597907 | 3.4:+72648735 | None:intergenic |
| ACTAACCTGGTCAAATCTGA+AGG | 0.603314 | 3.4:+72648298 | None:intergenic |
| AAACACAAAGAAGATTATGG+AGG | 0.618726 | 3.4:-72648482 | MS.gene29844:CDS |
| TCGCGGGGACACAAAGGTCA+TGG | 0.619216 | 3.4:-72648197 | MS.gene29844:CDS |
| CAATATATCTCAGTTACTCG+CGG | 0.625172 | 3.4:-72648214 | MS.gene29844:CDS |
| TGAATTGTTGCAGATGGTGA+GGG | 0.625659 | 3.4:-72648560 | MS.gene29844:intron |
| TGTTCTTGTGATAGTGACAC+TGG | 0.627385 | 3.4:+72649707 | None:intergenic |
| ATGAATTGTTGCAGATGGTG+AGG | 0.628754 | 3.4:-72648561 | MS.gene29844:intron |
| ATTGCCAATCCGAGGCGCTG+TGG | 0.630157 | 3.4:+72648771 | None:intergenic |
| AGTTACTCGCGGGGACACAA+AGG | 0.631514 | 3.4:-72648203 | MS.gene29844:CDS |
| CATACATGATTGCCAATCCG+AGG | 0.632178 | 3.4:+72648763 | None:intergenic |
| AACCTGGTCAAATCTGAAGG+AGG | 0.655670 | 3.4:+72648301 | None:intergenic |
| TTGCTAATGAAGCAGAGACA+GGG | 0.659274 | 3.4:-72649156 | MS.gene29844:CDS |
| CAATCATGTATGATGCACCC+GGG | 0.666450 | 3.4:-72648752 | MS.gene29844:CDS |
| TAAACCTGAAGAGATTACCT+GGG | 0.681766 | 3.4:-72649416 | MS.gene29844:intron |
| ATATATCTCAGTTACTCGCG+GGG | 0.688008 | 3.4:-72648212 | MS.gene29844:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTAAATATCAACATTTTA+CGG | + | chr3.4:72649308-72649327 | None:intergenic | 10.0% |
| !!! | GATAATATTTTTGTAATATA+TGG | + | chr3.4:72649160-72649179 | None:intergenic | 10.0% |
| !!! | TGAATGATATATATATTTTA+AGG | - | chr3.4:72648692-72648711 | MS.gene29844:intron | 10.0% |
| !! | CTTTATCTTATTATAATTCT+CGG | + | chr3.4:72649428-72649447 | None:intergenic | 15.0% |
| !!! | ATTTTAAGGATTTAAAGATG+AGG | - | chr3.4:72648706-72648725 | MS.gene29844:intron | 20.0% |
| !!! | GGTATTTAAAATCACAAAAT+TGG | - | chr3.4:72648455-72648474 | MS.gene29844:CDS | 20.0% |
| !!! | TGATTTTAAATACCTTGAAA+TGG | + | chr3.4:72648449-72648468 | None:intergenic | 20.0% |
| ! | AACAAAGCCTAAAATTGTTA+TGG | - | chr3.4:72648603-72648622 | MS.gene29844:intron | 25.0% |
| ! | AGAACATTGATCAAACTTAT+CGG | + | chr3.4:72649345-72649364 | None:intergenic | 25.0% |
| ! | ATGTCCAATAATTGAAATCT+TGG | - | chr3.4:72648278-72648297 | MS.gene29844:CDS | 25.0% |
| ! | TCAGATTGAATTTAAAGATG+AGG | - | chr3.4:72648643-72648662 | MS.gene29844:intron | 25.0% |
| ! | TGTTGAAAAAAAGCCTTAAA+TGG | - | chr3.4:72648500-72648519 | MS.gene29844:CDS | 25.0% |
| ! | TTTGTTCTCATTATAAGTAC+TGG | + | chr3.4:72648859-72648878 | None:intergenic | 25.0% |
| !!! | AATATTTTGAGTGAAGAATG+AGG | - | chr3.4:72648323-72648342 | MS.gene29844:CDS | 25.0% |
| !!! | AGTATGTTTTTTCTTGGTTA+AGG | - | chr3.4:72648765-72648784 | MS.gene29844:CDS | 25.0% |
| !!! | TTAACGAGTATGTTTTTTCT+TGG | - | chr3.4:72648759-72648778 | MS.gene29844:CDS | 25.0% |
| !!! | TTTTTTTTTACAGCAGAAGT+TGG | - | chr3.4:72649280-72649299 | MS.gene29844:intron | 25.0% |
| AAACACAAAGAAGATTATGG+AGG | - | chr3.4:72649468-72649487 | MS.gene29844:CDS | 30.0% | |
| AAAGAATGAATTGTTGCAGA+TGG | - | chr3.4:72649384-72649403 | MS.gene29844:intron | 30.0% | |
| AACACAAAGAAGATTATGGA+GGG | - | chr3.4:72649469-72649488 | MS.gene29844:CDS | 30.0% | |
| CCAAAACACAAAGAAGATTA+TGG | - | chr3.4:72649465-72649484 | MS.gene29844:CDS | 30.0% | |
| GAGATATATTGATCAGGTTT+AGG | + | chr3.4:72649727-72649746 | None:intergenic | 30.0% | |
| GATAGGAACATTATCAGATA+AGG | - | chr3.4:72648399-72648418 | MS.gene29844:CDS | 30.0% | |
| GTAACTGAGATATATTGATC+AGG | + | chr3.4:72649733-72649752 | None:intergenic | 30.0% | |
| TATATTCCTGTCTCCATTTA+AGG | + | chr3.4:72648516-72648535 | None:intergenic | 30.0% | |
| TATGGAGGGTTTATTTGATA+TGG | - | chr3.4:72649483-72649502 | MS.gene29844:CDS | 30.0% | |
| TGCTCCAAGATTTCAATTAT+TGG | + | chr3.4:72648285-72648304 | None:intergenic | 30.0% | |
| ! | AACACAGCCATAACAATTTT+AGG | + | chr3.4:72648613-72648632 | None:intergenic | 30.0% |
| ! | ATCAACTATTTTCTCGTTGT+TGG | + | chr3.4:72649695-72649714 | None:intergenic | 30.0% |
| ! | CCATAATCTTCTTTGTGTTT+TGG | + | chr3.4:72649468-72649487 | None:intergenic | 30.0% |
| !! | TAAGCATTTTGTTTACTGCA+TGG | - | chr3.4:72649025-72649044 | MS.gene29844:intron | 30.0% |
| AAAAAGCCTTAAATGGAGAC+AGG | - | chr3.4:72648507-72648526 | MS.gene29844:CDS | 35.0% | |
| AATATATCTCAGTTACTCGC+GGG | - | chr3.4:72649737-72649756 | MS.gene29844:CDS | 35.0% | |
| ACTTCAGCATGAGTGAAAAA+TGG | - | chr3.4:72648926-72648945 | MS.gene29844:CDS | 35.0% | |
| ATAAACCTGAAGAGATTACC+TGG | - | chr3.4:72648533-72648552 | MS.gene29844:CDS | 35.0% | |
| ATATGCTAAGAGAATGAGAG+AGG | - | chr3.4:72649561-72649580 | MS.gene29844:CDS | 35.0% | |
| CAATATATCTCAGTTACTCG+CGG | - | chr3.4:72649736-72649755 | MS.gene29844:CDS | 35.0% | |
| TAAACCTGAAGAGATTACCT+GGG | - | chr3.4:72648534-72648553 | MS.gene29844:CDS | 35.0% | |
| TATAATGAGAACAAATCGCC+AGG | - | chr3.4:72648864-72648883 | MS.gene29844:CDS | 35.0% | |
| TATTTCGTTACCGAGAAGAA+TGG | + | chr3.4:72649233-72649252 | None:intergenic | 35.0% | |
| ! | ACACTGGTAACATTTTCTCA+TGG | + | chr3.4:72648230-72648249 | None:intergenic | 35.0% |
| ! | AGTGATTTTCAGAAGAAACC+TGG | + | chr3.4:72648885-72648904 | None:intergenic | 35.0% |
| ! | ATAACAAGATGCACTCCTTT+TGG | - | chr3.4:72649587-72649606 | MS.gene29844:intron | 35.0% |
| ! | ATTGAGGTGAGAAAGTTGAT+AGG | - | chr3.4:72648382-72648401 | MS.gene29844:CDS | 35.0% |
| ! | GATTTTCCAACCATTCTTCT+CGG | - | chr3.4:72649220-72649239 | MS.gene29844:intron | 35.0% |
| AAATATTATCCACAGCGCCT+CGG | - | chr3.4:72649170-72649189 | MS.gene29844:intron | 40.0% | |
| AACTTGAACCTGCATTTGGT+GGG | - | chr3.4:72649509-72649528 | MS.gene29844:CDS | 40.0% | |
| AAGAATGGTTGGAAAATCCC+GGG | + | chr3.4:72649218-72649237 | None:intergenic | 40.0% | |
| ACATTGTGTGAGACTGATGA+TGG | + | chr3.4:72649628-72649647 | None:intergenic | 40.0% | |
| ACTAACCTGGTCAAATCTGA+AGG | + | chr3.4:72649655-72649674 | None:intergenic | 40.0% | |
| ACTTTCTCACCTCAATGATC+TGG | + | chr3.4:72648378-72648397 | None:intergenic | 40.0% | |
| AGAGTTTGCATGTGTCCAAA+AGG | + | chr3.4:72649605-72649624 | None:intergenic | 40.0% | |
| ATATATCTCAGTTACTCGCG+GGG | - | chr3.4:72649738-72649757 | MS.gene29844:CDS | 40.0% | |
| ATCAGAATCTGCTACTAACC+TGG | + | chr3.4:72649668-72649687 | None:intergenic | 40.0% | |
| ATGAATTGTTGCAGATGGTG+AGG | - | chr3.4:72649389-72649408 | MS.gene29844:intron | 40.0% | |
| ATTATTGTGCCAAGGATGGA+AGG | - | chr3.4:72648833-72648852 | MS.gene29844:CDS | 40.0% | |
| CACTTGACAAACTGAAACCA+TGG | + | chr3.4:72649109-72649128 | None:intergenic | 40.0% | |
| CAGAGACAGGGAAAATGTTT+AGG | - | chr3.4:72648806-72648825 | MS.gene29844:CDS | 40.0% | |
| CCAAATTATTGTGCCAAGGA+TGG | - | chr3.4:72648829-72648848 | MS.gene29844:CDS | 40.0% | |
| CCATCCTTGGCACAATAATT+TGG | + | chr3.4:72648832-72648851 | None:intergenic | 40.0% | |
| GAAGAATGGTTGGAAAATCC+CGG | + | chr3.4:72649219-72649238 | None:intergenic | 40.0% | |
| GATCAACTTGAACCTGCATT+TGG | - | chr3.4:72649505-72649524 | MS.gene29844:CDS | 40.0% | |
| GTTCTGATGCATCCATTTCA+AGG | - | chr3.4:72648434-72648453 | MS.gene29844:CDS | 40.0% | |
| TAGGCCAAATTATTGTGCCA+AGG | - | chr3.4:72648825-72648844 | MS.gene29844:CDS | 40.0% | |
| TCATTATTCCCACCAAATGC+AGG | + | chr3.4:72649520-72649539 | None:intergenic | 40.0% | |
| TGAATTGTTGCAGATGGTGA+GGG | - | chr3.4:72649390-72649409 | MS.gene29844:intron | 40.0% | |
| TGTTGCATTCCAGATCATTG+AGG | - | chr3.4:72648366-72648385 | MS.gene29844:CDS | 40.0% | |
| TTGCTAATGAAGCAGAGACA+GGG | - | chr3.4:72648794-72648813 | MS.gene29844:CDS | 40.0% | |
| ! | TGTTCTTGTGATAGTGACAC+TGG | + | chr3.4:72648246-72648265 | None:intergenic | 40.0% |
| AACCTGGTCAAATCTGAAGG+AGG | + | chr3.4:72649652-72649671 | None:intergenic | 45.0% | |
| AAGCAAGCAGTAACTTACCC+AGG | + | chr3.4:72648554-72648573 | None:intergenic | 45.0% | |
| ATCACAAGAACAGCAGGCTA+AGG | - | chr3.4:72648254-72648273 | MS.gene29844:CDS | 45.0% | |
| CAACTTGAACCTGCATTTGG+TGG | - | chr3.4:72649508-72649527 | MS.gene29844:CDS | 45.0% | |
| CAATCATGTATGATGCACCC+GGG | - | chr3.4:72649198-72649217 | MS.gene29844:intron | 45.0% | |
| CATACATGATTGCCAATCCG+AGG | + | chr3.4:72649190-72649209 | None:intergenic | 45.0% | |
| CTGAAGAAAACTGCCTCTGA+TGG | - | chr3.4:72648204-72648223 | MS.gene29844:CDS | 45.0% | |
| CTTACCCAGGTAATCTCTTC+AGG | + | chr3.4:72648541-72648560 | None:intergenic | 45.0% | |
| GCAATCATGTATGATGCACC+CGG | - | chr3.4:72649197-72649216 | MS.gene29844:intron | 45.0% | |
| GTCACTATCACAAGAACAGC+AGG | - | chr3.4:72648248-72648267 | MS.gene29844:CDS | 45.0% | |
| GTTGCTAATGAAGCAGAGAC+AGG | - | chr3.4:72648793-72648812 | MS.gene29844:CDS | 45.0% | |
| TCGTTACCGAGAAGAATGGT+TGG | + | chr3.4:72649229-72649248 | None:intergenic | 45.0% | |
| TTCCTCCTTCAGATTTGACC+AGG | - | chr3.4:72649647-72649666 | MS.gene29844:intron | 45.0% | |
| TTTAAATCTTCCCCCTCACC+AGG | - | chr3.4:72649058-72649077 | MS.gene29844:intron | 45.0% | |
| ! | ATCTGGCTGGTTGATTTCCA+TGG | - | chr3.4:72649089-72649108 | MS.gene29844:CDS | 45.0% |
| ! | CATTTTCTCATGGCCATCAG+AGG | + | chr3.4:72648220-72648239 | None:intergenic | 45.0% |
| !! | AAGTACTGGCCTTCCATCCT+TGG | + | chr3.4:72648845-72648864 | None:intergenic | 50.0% |
| AGATCACCTGCTCCTGGTGA+GGG | + | chr3.4:72649073-72649092 | None:intergenic | 55.0% | |
| AGTTACTCGCGGGGACACAA+AGG | - | chr3.4:72649747-72649766 | MS.gene29844:CDS | 55.0% | |
| TTATCCACAGCGCCTCGGAT+TGG | - | chr3.4:72649175-72649194 | MS.gene29844:intron | 55.0% | |
| ATCACCTGCTCCTGGTGAGG+GGG | + | chr3.4:72649071-72649090 | None:intergenic | 60.0% | |
| CAGATCACCTGCTCCTGGTG+AGG | + | chr3.4:72649074-72649093 | None:intergenic | 60.0% | |
| CTCACCAGGAGCAGGTGATC+TGG | - | chr3.4:72649072-72649091 | MS.gene29844:intron | 60.0% | |
| GATCACCTGCTCCTGGTGAG+GGG | + | chr3.4:72649072-72649091 | None:intergenic | 60.0% | |
| TCGCGGGGACACAAAGGTCA+TGG | - | chr3.4:72649753-72649772 | MS.gene29844:CDS | 60.0% | |
| !! | ATTGCCAATCCGAGGCGCTG+TGG | + | chr3.4:72649182-72649201 | None:intergenic | 60.0% |
| CCAGCCAGATCACCTGCTCC+TGG | + | chr3.4:72649079-72649098 | None:intergenic | 65.0% | |
| CCAGGAGCAGGTGATCTGGC+TGG | - | chr3.4:72649076-72649095 | MS.gene29844:intron | 65.0% | |
| TCTTCCCCCTCACCAGGAGC+AGG | - | chr3.4:72649064-72649083 | MS.gene29844:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 72648192 | 72649780 | 72648192 | ID=MS.gene29844 |
| chr3.4 | mRNA | 72648192 | 72649780 | 72648192 | ID=MS.gene29844.t1;Parent=MS.gene29844 |
| chr3.4 | exon | 72649697 | 72649780 | 72649697 | ID=MS.gene29844.t1.exon1;Parent=MS.gene29844.t1 |
| chr3.4 | CDS | 72649697 | 72649780 | 72649697 | ID=cds.MS.gene29844.t1;Parent=MS.gene29844.t1 |
| chr3.4 | exon | 72649417 | 72649593 | 72649417 | ID=MS.gene29844.t1.exon2;Parent=MS.gene29844.t1 |
| chr3.4 | CDS | 72649417 | 72649593 | 72649417 | ID=cds.MS.gene29844.t1;Parent=MS.gene29844.t1 |
| chr3.4 | exon | 72649087 | 72649185 | 72649087 | ID=MS.gene29844.t1.exon3;Parent=MS.gene29844.t1 |
| chr3.4 | CDS | 72649087 | 72649185 | 72649087 | ID=cds.MS.gene29844.t1;Parent=MS.gene29844.t1 |
| chr3.4 | exon | 72648731 | 72648973 | 72648731 | ID=MS.gene29844.t1.exon4;Parent=MS.gene29844.t1 |
| chr3.4 | CDS | 72648731 | 72648973 | 72648731 | ID=cds.MS.gene29844.t1;Parent=MS.gene29844.t1 |
| chr3.4 | exon | 72648192 | 72648569 | 72648192 | ID=MS.gene29844.t1.exon5;Parent=MS.gene29844.t1 |
| chr3.4 | CDS | 72648192 | 72648569 | 72648192 | ID=cds.MS.gene29844.t1;Parent=MS.gene29844.t1 |
| Gene Sequence |
| Protein sequence |
