AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95806


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95806.t1 MTR_1g103050 98.611 72 1 0 1 72 1 72 3.90e-48 149
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 16 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCATCCAATGATTCCTTT+AGG 0.352281 1.1:+73974790 None:intergenic
CTCTTACCTAAAGGAATCAT+TGG 0.362848 1.1:-73974796 MS.gene95806:CDS
TTTGAAAGCAGTTTCATCTA+AGG 0.366099 1.1:+73974746 None:intergenic
TTTCTTCTGTAGGCGTTGTT+TGG 0.381475 1.1:+73974722 None:intergenic
TGAATGGCTGGTAGGCTACC+TGG 0.496595 1.1:-73974891 MS.gene95806:CDS
CAACGCCTACAGAAGAAACT+TGG 0.500663 1.1:-73974717 MS.gene95806:CDS
GCTGGTAGGCTACCTGGCGT+TGG 0.518244 1.1:-73974885 MS.gene95806:CDS
GGATTTGATGTACCTGGAAG+AGG 0.518796 1.1:+73974683 None:intergenic
GGTAAAGAAGAGTGAATGGC+TGG 0.520572 1.1:-73974903 None:intergenic
CTACAGAGACTCTTACCTAA+AGG 0.529350 1.1:-73974805 MS.gene95806:CDS
AGTGACCAAGTTTCTTCTGT+AGG 0.561380 1.1:+73974712 None:intergenic
CTTGGTGACAATCCAACGCC+AGG 0.563291 1.1:+73974873 None:intergenic
GTAGTAGTCATTGAAGTCCA+TGG 0.592320 1.1:+73974768 None:intergenic
AAGAAGAGTGAATGGCTGGT+AGG 0.606425 1.1:-73974899 None:intergenic
CTTTAGGTAAGAGTCTCTGT+AGG 0.619274 1.1:+73974806 None:intergenic
AGGAATCATTGGATGCACCA+TGG 0.643184 1.1:-73974785 MS.gene95806:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAACTGTATTATTATGT+AGG + chr1.1:73974729-73974748 None:intergenic 15.0%
!!! ATTTATTTTTTTTCTCTCTA+AGG - chr1.1:73974885-73974904 MS.gene95806:CDS 15.0%
!! AGAATTTATACAAATTGTCT+AGG - chr1.1:73974803-73974822 MS.gene95806:CDS 20.0%
!!! TAATTTTCTGATCTTTTTCT+TGG + chr1.1:73974536-73974555 None:intergenic 20.0%
!!! TTTTTGAAGAAACAAGAAAT+TGG - chr1.1:73974752-73974771 MS.gene95806:CDS 20.0%
!! TGTATAAATTCTAGCTAGTT+AGG + chr1.1:73974795-73974814 None:intergenic 25.0%
TTTGAAAGCAGTTTCATCTA+AGG + chr1.1:73974645-73974664 None:intergenic 30.0%
CTCTTACCTAAAGGAATCAT+TGG - chr1.1:73974592-73974611 MS.gene95806:intron 35.0%
GAGTGTGCATAAGAAATATG+TGG + chr1.1:73974844-73974863 None:intergenic 35.0%
TATGTAGGATTTGATGTACC+TGG + chr1.1:73974714-73974733 None:intergenic 35.0%
AGTGACCAAGTTTCTTCTGT+AGG + chr1.1:73974679-73974698 None:intergenic 40.0%
CTACAGAGACTCTTACCTAA+AGG - chr1.1:73974583-73974602 MS.gene95806:intron 40.0%
CTTTAGGTAAGAGTCTCTGT+AGG + chr1.1:73974585-73974604 None:intergenic 40.0%
GTAGTAGTCATTGAAGTCCA+TGG + chr1.1:73974623-73974642 None:intergenic 40.0%
GTGCATCCAATGATTCCTTT+AGG + chr1.1:73974601-73974620 None:intergenic 40.0%
! TTTCTTCTGTAGGCGTTGTT+TGG + chr1.1:73974669-73974688 None:intergenic 40.0%
AGGAATCATTGGATGCACCA+TGG - chr1.1:73974603-73974622 MS.gene95806:intron 45.0%
CAACGCCTACAGAAGAAACT+TGG - chr1.1:73974671-73974690 MS.gene95806:intron 45.0%
GCACACTCACATGCATAGTA+AGG - chr1.1:73974856-73974875 MS.gene95806:CDS 45.0%
GGATTTGATGTACCTGGAAG+AGG + chr1.1:73974708-73974727 None:intergenic 45.0%
! GTCACTTCTTTTCCTCTTCC+AGG - chr1.1:73974693-73974712 MS.gene95806:intron 45.0%
! CTTGGTGACAATCCAACGCC+AGG + chr1.1:73974518-73974537 None:intergenic 55.0%
GCTGGTAGGCTACCTGGCGT+TGG - chr1.1:73974503-73974522 MS.gene95806:intron 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 73974500 73974910 73974500 ID=MS.gene95806
chr1.1 mRNA 73974500 73974910 73974500 ID=MS.gene95806.t1;Parent=MS.gene95806
chr1.1 exon 73974696 73974910 73974696 ID=MS.gene95806.t1.exon1;Parent=MS.gene95806.t1
chr1.1 CDS 73974696 73974910 73974696 ID=cds.MS.gene95806.t1;Parent=MS.gene95806.t1
chr1.1 exon 73974500 73974503 73974500 ID=MS.gene95806.t1.exon2;Parent=MS.gene95806.t1
chr1.1 CDS 73974500 73974503 73974500 ID=cds.MS.gene95806.t1;Parent=MS.gene95806.t1
Gene Sequence

>MS.gene95806

ATGGCTGGTAGGCTACCTGGCGTTGGATTGTCACCAAGAAAAAGATCAGAAAATTATCACAACAGACATGAACATTATGCTACCTACAGAGACTCTTACCTAAAGGAATCATTGGATGCACCATGGACTTCAATGACTACTACCTTAGATGAAACTGCTTTCAAAGCCAAACAACGCCTACAGAAGAAACTTGGTCACTTCTTTTCCTCTTCCAGGTACATCAAATCCTACATAATAATACAGTTTTTTTATTTTTTGAAGAAACAAGAAATTGGTTACTATTAATTTTGAGCCTAACTAGCTAGAATTTATACAAATTGTCTAGGCTTTTATTTAATTTTCCACATATTTCTTATGCACACTCACATGCATAGTAAGGAATTTAATTTATTTTTTTTCTCTCTAAGGTGA

Protein sequence

>MS.gene95806.t1

MAGRLPGVGLSPRKRSENYHNRHEHYATYRDSYLKESLDAPWTSMTTTLDETAFKAKQRLQKKLGHFFSSSR