AlfalfaGEDB | MS.gene066500

M. sativa cultivar XinJiangDaYe / MS.gene066500

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12796.10 PF13857.9 PF13962.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066500.t1	RHN41843.1	88.3	342	39	1	1	341	1	342	2.90E-104	388.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066500.t1	Q8GYH5	48.0	306	150	3	7	305	1	304	5.0e-12	73.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066500.t1	G7L8Q1	88.3	342	39	1	1	341	1	342	2.1e-104	388.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene066500	-0.800266	1.56E-48	-1.69E-46
MS.gene049549	MS.gene066500	0.817327	3.49E-52	-1.69E-46
MS.gene050095	MS.gene066500	0.80297	4.36E-49	-1.69E-46
MS.gene050647	MS.gene066500	0.801916	7.19E-49	-1.69E-46
MS.gene050720	MS.gene066500	0.814657	1.38E-51	-1.69E-46
MS.gene050747	MS.gene066500	-0.800467	1.42E-48	-1.69E-46
MS.gene05104	MS.gene066500	0.802166	6.38E-49	-1.69E-46
MS.gene051131	MS.gene066500	0.834083	3.68E-56	-1.69E-46
MS.gene051138	MS.gene066500	0.801686	8.01E-49	-1.69E-46
MS.gene051158	MS.gene066500	0.800712	1.27E-48	-1.69E-46
MS.gene051996	MS.gene066500	0.806777	6.98E-50	-1.69E-46
MS.gene052211	MS.gene066500	0.858698	6.89E-63	-1.69E-46
MS.gene052212	MS.gene066500	0.839381	1.64E-57	-1.69E-46
MS.gene05240	MS.gene066500	0.857543	1.52E-62	-1.69E-46
MS.gene05244	MS.gene066500	0.816559	5.20E-52	-1.69E-46
MS.gene052606	MS.gene066500	0.820612	6.25E-53	-1.69E-46
MS.gene053403	MS.gene066500	0.819441	1.16E-52	-1.69E-46
MS.gene053406	MS.gene066500	0.863444	2.48E-64	-1.69E-46
MS.gene053420	MS.gene066500	0.804822	1.80E-49	-1.69E-46
MS.gene053574	MS.gene066500	-0.80168	8.03E-49	-1.69E-46
MS.gene054146	MS.gene066500	0.855902	4.62E-62	-1.69E-46
MS.gene054421	MS.gene066500	0.812933	3.30E-51	-1.69E-46
MS.gene05470	MS.gene066500	-0.806177	9.34E-50	-1.69E-46
MS.gene054742	MS.gene066500	0.825564	4.38E-54	-1.69E-46
MS.gene054884	MS.gene066500	0.808431	3.11E-50	-1.69E-46
MS.gene055240	MS.gene066500	0.835544	1.58E-56	-1.69E-46
MS.gene05589	MS.gene066500	-0.800178	1.63E-48	-1.69E-46
MS.gene056218	MS.gene066500	0.830173	3.41E-55	-1.69E-46
MS.gene056590	MS.gene066500	0.800193	1.62E-48	-1.69E-46
MS.gene056591	MS.gene066500	0.802389	5.74E-49	-1.69E-46
MS.gene056748	MS.gene066500	0.813724	2.21E-51	-1.69E-46
MS.gene056920	MS.gene066500	0.805949	1.04E-49	-1.69E-46
MS.gene056922	MS.gene066500	0.800332	1.51E-48	-1.69E-46
MS.gene057261	MS.gene066500	0.822704	2.05E-53	-1.69E-46
MS.gene057820	MS.gene066500	0.801766	7.71E-49	-1.69E-46
MS.gene05837	MS.gene066500	-0.809816	1.57E-50	-1.69E-46
MS.gene058544	MS.gene066500	-0.813089	3.05E-51	-1.69E-46
MS.gene058603	MS.gene066500	0.820604	6.28E-53	-1.69E-46
MS.gene058838	MS.gene066500	-0.802855	4.60E-49	-1.69E-46
MS.gene059265	MS.gene066500	0.813072	3.08E-51	-1.69E-46
MS.gene059892	MS.gene066500	0.810434	1.16E-50	-1.69E-46
MS.gene059953	MS.gene066500	0.826193	3.10E-54	-1.69E-46
MS.gene059955	MS.gene066500	0.855153	7.64E-62	-1.69E-46
MS.gene060087	MS.gene066500	0.823621	1.25E-53	-1.69E-46
MS.gene06077	MS.gene066500	-0.802027	6.82E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene30172	MS.gene066500	PPI
MS.gene23852	MS.gene066500	PPI
MS.gene69966	MS.gene066500	PPI
MS.gene066500	MS.gene81055	PPI
MS.gene97943	MS.gene066500	PPI
MS.gene066500	MS.gene65775	PPI
MS.gene066500	MS.gene32723	PPI
MS.gene066500	MS.gene80163	PPI
MS.gene066500	MS.gene02469	PPI
MS.gene027071	MS.gene066500	PPI
MS.gene97379	MS.gene066500	PPI
MS.gene67157	MS.gene066500	PPI
MS.gene066500	MS.gene045937	PPI
MS.gene30433	MS.gene066500	PPI
MS.gene012203	MS.gene066500	PPI
MS.gene022109	MS.gene066500	PPI
MS.gene34651	MS.gene066500	PPI
MS.gene25244	MS.gene066500	PPI
MS.gene066500	MS.gene51278	PPI
MS.gene010910	MS.gene066500	PPI
MS.gene066500	MS.gene87218	PPI
MS.gene025229	MS.gene066500	PPI
MS.gene066500	MS.gene85534	PPI
MS.gene063222	MS.gene066500	PPI
MS.gene25515	MS.gene066500	PPI
MS.gene07706	MS.gene066500	PPI
MS.gene027074	MS.gene066500	PPI
MS.gene066500	MS.gene23881	PPI
MS.gene69687	MS.gene066500	PPI
MS.gene066500	MS.gene014931	PPI
MS.gene066500	MS.gene062662	PPI
MS.gene23810	MS.gene066500	PPI
MS.gene066500	MS.gene30177	PPI
MS.gene066500	MS.gene70008	PPI
MS.gene024753	MS.gene066500	PPI
MS.gene066500	MS.gene90400	PPI
MS.gene78281	MS.gene066500	PPI
MS.gene005995	MS.gene066500	PPI
MS.gene037950	MS.gene066500	PPI
MS.gene70008	MS.gene066500	PPI
MS.gene066500	MS.gene09785	PPI
MS.gene64441	MS.gene066500	PPI
MS.gene24806	MS.gene066500	PPI
MS.gene071113	MS.gene066500	PPI
MS.gene004844	MS.gene066500	PPI
MS.gene30176	MS.gene066500	PPI
MS.gene066500	MS.gene027072	PPI
MS.gene066500	MS.gene65463	PPI
MS.gene007487	MS.gene066500	PPI
MS.gene066500	MS.gene007485	PPI
MS.gene018518	MS.gene066500	PPI
MS.gene066464	MS.gene066500	PPI
MS.gene066500	MS.gene067705	PPI
MS.gene24922	MS.gene066500	PPI
MS.gene027073	MS.gene066500	PPI
MS.gene066500	MS.gene043742	PPI
MS.gene066500	MS.gene027441	PPI
MS.gene010912	MS.gene066500	PPI
MS.gene066500	MS.gene050457	PPI
MS.gene066500	MS.gene99973	PPI
MS.gene25024	MS.gene066500	PPI
MS.gene23811	MS.gene066500	PPI
MS.gene066500	MS.gene30174	PPI
MS.gene34712	MS.gene066500	PPI
MS.gene066500	MS.gene008833	PPI
MS.gene51335	MS.gene066500	PPI
MS.gene066500	MS.gene48655	PPI
MS.gene068249	MS.gene066500	PPI
MS.gene066046	MS.gene066500	PPI
MS.gene051003	MS.gene066500	PPI
MS.gene27223	MS.gene066500	PPI
MS.gene066500	MS.gene050270	PPI
MS.gene066500	MS.gene065848	PPI
MS.gene066500	MS.gene037184	PPI
MS.gene22482	MS.gene066500	PPI
MS.gene066500	MS.gene23196	PPI
MS.gene039682	MS.gene066500	PPI
MS.gene061932	MS.gene066500	PPI
MS.gene066500	MS.gene038519	PPI
MS.gene26532	MS.gene066500	PPI
MS.gene064542	MS.gene066500	PPI
MS.gene066467	MS.gene066500	PPI
MS.gene47479	MS.gene066500	PPI
MS.gene21734	MS.gene066500	PPI
MS.gene33377	MS.gene066500	PPI
MS.gene008767	MS.gene066500	PPI
MS.gene066500	MS.gene26021	PPI
MS.gene71204	MS.gene066500	PPI
MS.gene007486	MS.gene066500	PPI
MS.gene041770	MS.gene066500	PPI
MS.gene066500	MS.gene050456	PPI
MS.gene066500	MS.gene51258	PPI
MS.gene26672	MS.gene066500	PPI
MS.gene36842	MS.gene066500	PPI
MS.gene066500	MS.gene053363	PPI
MS.gene066500	MS.gene91775	PPI
MS.gene99554	MS.gene066500	PPI
MS.gene058128	MS.gene066500	PPI
MS.gene99588	MS.gene066500	PPI
MS.gene027075	MS.gene066500	PPI
MS.gene41291	MS.gene066500	PPI
MS.gene066500	MS.gene32726	PPI
MS.gene066500	MS.gene71122	PPI
MS.gene066501	MS.gene066500	PPI
MS.gene08470	MS.gene066500	PPI
MS.gene63085	MS.gene066500	PPI
MS.gene066500	MS.gene052956	PPI
MS.gene056455	MS.gene066500	PPI
MS.gene066500	MS.gene60920	PPI
MS.gene066500	MS.gene73119	PPI
MS.gene066500	MS.gene92243	PPI
MS.gene89986	MS.gene066500	PPI
MS.gene26019	MS.gene066500	PPI
MS.gene066500	MS.gene34009	PPI
MS.gene066500	MS.gene51262	PPI
MS.gene066500	MS.gene043662	PPI
MS.gene052956	MS.gene066500	PPI
MS.gene035805	MS.gene066500	PPI
MS.gene066500	MS.gene99588	PPI
MS.gene066500	MS.gene21888	PPI
MS.gene066500	MS.gene54764	PPI
MS.gene69965	MS.gene066500	PPI
MS.gene066500	MS.gene27223	PPI
MS.gene066500	MS.gene39623	PPI
MS.gene82740	MS.gene066500	PPI
MS.gene042783	MS.gene066500	PPI
MS.gene066500	MS.gene050272	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066500.t1	MTR_8g073260	92.982	342	23	1	1	341	1	342	0.0	650
MS.gene066500.t1	MTR_3g016210	61.905	357	117	4	1	339	1	356	1.31e-154	441
MS.gene066500.t1	MTR_8g072510	63.265	343	122	3	1	340	1	342	2.89e-152	436
MS.gene066500.t1	MTR_8g039620	63.897	349	117	5	1	340	1	349	3.80e-152	435
MS.gene066500.t1	MTR_1g059930	64.286	336	116	1	10	341	11	346	6.62e-151	432
MS.gene066500.t1	MTR_7g451420	66.343	309	104	0	10	318	8	316	1.86e-145	413
MS.gene066500.t1	MTR_8g073275	63.231	359	52	7	53	341	1	349	4.02e-141	407
MS.gene066500.t1	MTR_7g021090	58.017	343	132	4	1	339	1	335	6.03e-136	393
MS.gene066500.t1	MTR_8g068990	59.644	337	116	5	10	340	11	333	4.19e-132	385
MS.gene066500.t1	MTR_8g062410	60.000	345	125	6	1	340	1	337	3.83e-129	377
MS.gene066500.t1	MTR_8g069880	59.184	343	134	5	4	342	1	341	8.23e-129	377
MS.gene066500.t1	MTR_8g467780	57.402	331	131	1	1	321	1	331	7.18e-127	371
MS.gene066500.t1	MTR_8g066670	60.417	336	128	4	9	340	1073	1407	6.63e-124	389
MS.gene066500.t1	MTR_8g040930	60.678	295	110	3	53	341	1	295	5.13e-121	353
MS.gene066500.t1	MTR_8g041240	60.678	295	110	3	53	341	1	295	5.13e-121	353
MS.gene066500.t1	MTR_3g105650	53.333	315	136	3	4	311	1	311	3.75e-116	343
MS.gene066500.t1	MTR_1g069110	27.193	342	202	9	14	340	21	330	3.52e-27	111
MS.gene066500.t1	MTR_7g099580	25.449	334	189	9	14	333	15	302	5.88e-25	103
MS.gene066500.t1	MTR_7g099570	25.969	258	167	5	14	256	12	260	2.18e-18	86.7
MS.gene066500.t1	MTR_2g101320	27.982	218	139	4	39	248	134	341	5.42e-18	85.5
MS.gene066500.t1	MTR_7g117620	26.481	287	177	7	46	310	119	393	5.76e-17	82.0
MS.gene066500.t1	MTR_1g115575	25.753	299	181	6	46	318	169	452	7.37e-17	82.0
MS.gene066500.t1	MTR_1g032100	26.140	329	180	11	47	340	95	395	2.71e-15	77.0
MS.gene066500.t1	MTR_4g062100	24.653	288	181	6	47	310	109	384	3.71e-15	76.6
MS.gene066500.t1	MTR_7g100430	26.415	318	180	12	44	311	124	437	3.99e-15	76.6
MS.gene066500.t1	MTR_2g101310	26.728	217	139	4	42	249	137	342	6.30e-15	75.9
MS.gene066500.t1	MTR_2g101310	26.852	216	140	4	42	249	137	342	6.41e-15	76.3
MS.gene066500.t1	MTR_2g096190	25.466	322	168	9	13	310	107	380	8.12e-15	75.9
MS.gene066500.t1	MTR_8g105260	23.793	290	184	5	46	310	101	378	3.37e-12	67.8
MS.gene066500.t1	MTR_7g100390	24.611	321	171	7	13	310	90	362	3.77e-12	67.4
MS.gene066500.t1	MTR_5g085500	29.114	158	100	4	80	233	8	157	1.67e-11	65.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066500.t1	AT4G10720	45.511	323	168	2	7	321	1	323	3.12e-96	291
MS.gene066500.t1	AT4G10720	44.315	343	174	3	7	341	1	334	5.41e-96	292
MS.gene066500.t1	AT1G14500	40.841	333	188	2	7	330	1	333	1.03e-84	263
MS.gene066500.t1	AT1G14480	40.000	325	185	2	7	321	1	325	5.01e-78	246
MS.gene066500.t1	AT5G15500	41.471	340	184	5	11	342	6	338	9.64e-78	246
MS.gene066500.t1	AT5G54620	41.987	312	168	5	7	308	1	309	4.84e-75	238
MS.gene066500.t1	AT5G54610	40.523	306	173	3	7	305	1	304	3.00e-73	233
MS.gene066500.t1	AT1G14480	38.279	337	168	7	7	338	1	302	2.44e-59	196
MS.gene066500.t1	AT5G15500	36.820	239	136	5	112	342	1	232	4.67e-38	139
MS.gene066500.t1	AT5G51160	31.673	281	160	6	59	317	31	301	9.39e-38	140
MS.gene066500.t1	AT1G10340	25.000	368	166	10	45	314	73	428	8.75e-18	84.7
MS.gene066500.t1	AT1G10340	30.000	240	120	7	111	314	205	432	2.09e-17	83.6
MS.gene066500.t1	AT4G03500	23.988	321	155	9	45	311	217	502	1.38e-15	78.2
MS.gene066500.t1	AT4G03500	23.988	321	155	9	45	311	194	479	1.55e-15	78.2
MS.gene066500.t1	AT4G03500	23.988	321	155	9	45	311	194	479	1.55e-15	78.2
MS.gene066500.t1	AT3G09550	25.773	291	175	7	47	311	182	457	2.24e-15	77.4
MS.gene066500.t1	AT3G09550	25.773	291	175	7	47	311	182	457	2.24e-15	77.4
MS.gene066500.t1	AT3G09550	25.773	291	175	7	47	311	189	464	2.50e-15	77.4
MS.gene066500.t1	AT2G24600	27.338	278	161	9	70	314	163	432	3.34e-15	77.0
MS.gene066500.t1	AT2G24600	27.338	278	161	9	70	314	163	432	3.34e-15	77.0
MS.gene066500.t1	AT2G24600	27.338	278	161	9	70	314	163	432	6.20e-15	76.3
MS.gene066500.t1	AT2G24600	27.338	278	161	9	70	314	163	432	6.20e-15	76.3
MS.gene066500.t1	AT5G54710	26.877	253	130	9	97	314	219	451	1.67e-14	75.1
MS.gene066500.t1	AT4G03460	27.468	233	115	7	104	316	292	490	1.83e-14	74.7
MS.gene066500.t1	AT5G54710	26.877	253	130	9	97	314	264	496	1.87e-14	74.7
MS.gene066500.t1	AT4G03460	27.468	233	115	7	104	316	260	458	2.19e-14	74.7
MS.gene066500.t1	AT1G34050	24.903	257	171	6	70	314	177	423	2.22e-14	74.3
MS.gene066500.t1	AT4G03490	26.518	313	159	12	45	311	180	467	2.63e-14	74.3
MS.gene066500.t1	AT3G12360	24.340	341	173	11	44	322	130	447	2.64e-14	74.3
MS.gene066500.t1	AT3G12360	24.340	341	173	11	44	322	118	435	2.65e-14	74.3
MS.gene066500.t1	AT2G01680	23.548	310	199	6	46	329	97	394	3.20e-14	73.9
MS.gene066500.t1	AT4G03490	26.518	313	159	12	45	311	180	467	4.03e-14	73.9
MS.gene066500.t1	AT4G03490	26.518	313	159	12	45	311	170	457	4.41e-14	73.6
MS.gene066500.t1	AT5G54700	26.531	294	139	9	26	314	20	241	5.21e-14	73.2
MS.gene066500.t1	AT2G31820	24.390	287	183	6	46	310	227	501	5.41e-14	73.6
MS.gene066500.t1	AT1G05640	23.580	352	184	9	44	310	114	465	5.87e-14	73.2
MS.gene066500.t1	AT5G02620	24.742	291	180	6	47	310	95	373	8.31e-14	72.8
MS.gene066500.t1	AT5G02620	24.742	291	180	6	47	310	95	373	8.31e-14	72.8
MS.gene066500.t1	AT5G02620	24.742	291	180	6	47	310	95	373	8.31e-14	72.8
MS.gene066500.t1	AT5G50140	26.829	205	124	5	42	230	26	220	1.34e-13	72.0
MS.gene066500.t1	AT1G07710	25.087	287	180	8	47	310	105	379	2.16e-11	65.5
MS.gene066500.t1	AT1G07710	25.087	287	180	8	47	310	105	379	2.16e-11	65.5
MS.gene066500.t1	AT5G60070	26.623	154	110	2	13	165	114	265	3.80e-11	64.7

Find 59 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTAACATCTTCGATCGAATT+TGG	0.197403	8.2:-31010805	None:intergenic
TCATAATTTCAGTGGCAAAT+TGG	0.236402	8.2:-31010570	None:intergenic
GAAATTAATACTTAATTATA+AGG	0.240344	8.2:+31010942	MS.gene066500:CDS
AGCACTAAACAATGACTTAA+AGG	0.246988	8.2:+31010996	MS.gene066500:CDS
CACCACTTCTTTGATTTCTA+AGG	0.264459	8.2:+31011820	MS.gene066500:CDS
TGAGGAGGATCCATATGTTT+TGG	0.280435	8.2:+31010483	MS.gene066500:CDS
TCTTAATTTATCTTCAAGTT+TGG	0.313621	8.2:-31011609	None:intergenic
TAGTAATATACCCTTGATTT+CGG	0.324028	8.2:-31011546	None:intergenic
CTCTTCATGTCTTGGTTGGT+TGG	0.334379	8.2:+31010881	MS.gene066500:CDS
AACGAAGCACCATACTCTTT+TGG	0.338315	8.2:-31010666	None:intergenic
TCGTTTCGTAGACATGAATA+AGG	0.350127	8.2:+31010684	MS.gene066500:CDS
TCTATATATTCCAAAACATA+TGG	0.396171	8.2:-31010493	None:intergenic
GCAAAACAACCAAAAGAGTA+TGG	0.398876	8.2:+31010657	MS.gene066500:CDS
GATTGAAATCTTCATTTGCT+TGG	0.413951	8.2:+31010593	MS.gene066500:CDS
GTTTGAAGCTCTTCATGTCT+TGG	0.417575	8.2:+31010873	MS.gene066500:CDS
AGCAGCAAGTGCCGAAATCA+AGG	0.421794	8.2:+31011535	MS.gene066500:CDS
GAAGCTCTTCATGTCTTGGT+TGG	0.429930	8.2:+31010877	MS.gene066500:CDS
TGTAGAGGAGTTTCAACAAA+TGG	0.445043	8.2:-31010523	None:intergenic
GTGAAGGTTGCAACTCTAGA+AGG	0.445366	8.2:+31010433	MS.gene066500:CDS
TCTACATATAGCTGCATCAA+TGG	0.456263	8.2:+31010540	MS.gene066500:CDS
TCTTTGATTTCTAAGGAAGT+TGG	0.463599	8.2:+31011827	MS.gene066500:CDS
CCAACTCCCTAGCACCTCTC+TGG	0.465296	8.2:-31010918	None:intergenic
CTTAATTATAAGGATGAGAA+AGG	0.474353	8.2:+31010952	MS.gene066500:CDS
CTTTGCATTCAAATTTACCT+TGG	0.475404	8.2:-31011483	None:intergenic
AAGGAACTTGTTAGAATCAA+AGG	0.479455	8.2:+31010703	MS.gene066500:CDS
CTTCATCTTGCTTGTCAAAG+TGG	0.481775	8.2:+31010745	MS.gene066500:CDS
CCAGAGAGGTGCTAGGGAGT+TGG	0.487334	8.2:+31010918	MS.gene066500:CDS
TCCACAGGCGCTTCGACTGT+TGG	0.498523	8.2:+31011454	MS.gene066500:intron
CTACATATAGCTGCATCAAT+GGG	0.510700	8.2:+31010541	MS.gene066500:CDS
AAGGGTATATTACTAAGTGC+TGG	0.522957	8.2:+31011554	MS.gene066500:CDS
GGTAAATCAGTTATTTCTGA+AGG	0.526141	8.2:+31011848	MS.gene066500:CDS
TTCCTTAGAAATCAAAGAAG+TGG	0.529376	8.2:-31011822	None:intergenic
CAAATCGCCAGAGAGGTGCT+AGG	0.534314	8.2:+31010911	MS.gene066500:CDS
GTTAGAATCAAAGGAAGGGA+AGG	0.542029	8.2:+31010712	MS.gene066500:CDS
GGAGTTTATCAAGGTAATGC+AGG	0.543139	8.2:+31011776	MS.gene066500:CDS
AAATCGCCAGAGAGGTGCTA+GGG	0.545317	8.2:+31010912	MS.gene066500:CDS
CCTTCTCTATACACTAATTG+AGG	0.550283	8.2:+31010465	MS.gene066500:CDS
TTTCAATCTCATAATTTCAG+TGG	0.552567	8.2:-31010578	None:intergenic
CCAAGCTCAAAAGTCAAAGA+TGG	0.559102	8.2:+31011584	MS.gene066500:CDS
CTTTGACAAGCAAGATGAAG+AGG	0.564320	8.2:-31010742	None:intergenic
GATCGAAGATGTTACTCTGA+GGG	0.576263	8.2:+31010813	MS.gene066500:CDS
CCTCAATTAGTGTATAGAGA+AGG	0.577645	8.2:-31010465	None:intergenic
AACTTGTTAGAATCAAAGGA+AGG	0.583415	8.2:+31010707	MS.gene066500:CDS
TTACCTTGATAAACTCCACC+AGG	0.585352	8.2:-31011770	None:intergenic
GCTTGGAAGCTAAATCAACA+AGG	0.604414	8.2:+31010610	MS.gene066500:CDS
ACTTGTTAGAATCAAAGGAA+GGG	0.615429	8.2:+31010708	MS.gene066500:CDS
GCAGCAAGTGCCGAAATCAA+GGG	0.615626	8.2:+31011536	MS.gene066500:CDS
TGATATTGATTACAAAGTGA+AGG	0.624755	8.2:+31010417	MS.gene066500:CDS
CTTGGAAGCTAAATCAACAA+GGG	0.626538	8.2:+31010611	MS.gene066500:CDS
ATTGATGCAGCTATATGTAG+AGG	0.639713	8.2:-31010538	None:intergenic
CGATCGAAGATGTTACTCTG+AGG	0.644610	8.2:+31010812	MS.gene066500:CDS
ATCGAAGATGTTACTCTGAG+GGG	0.654189	8.2:+31010814	MS.gene066500:CDS
TCTCTATACACTAATTGAGG+AGG	0.662445	8.2:+31010468	MS.gene066500:CDS
TACCTTGATAAACTCCACCA+GGG	0.669953	8.2:-31011769	None:intergenic
TCGACTGTTGGTAAAGACCA+AGG	0.673714	8.2:+31011466	MS.gene066500:CDS
ACCTTGATAAACTCCACCAG+GGG	0.689637	8.2:-31011768	None:intergenic
TATTAGAAAAGATATATCAG+AGG	0.690330	8.2:+31011676	MS.gene066500:CDS
TTGAAGACAAATCGCCAGAG+AGG	0.694700	8.2:+31010904	MS.gene066500:CDS
CCTTGATAAACTCCACCAGG+GGG	0.739127	8.2:-31011767	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAAAATATACTAAATAGA+AGG	-	chr8.2:31011025-31011044	None:intergenic	10.0%
!!	GAAATTAATACTTAATTATA+AGG	+	chr8.2:31010942-31010961	MS.gene066500:CDS	10.0%
!!!	TTATTTTTGATGAATAACTA+TGG	+	chr8.2:31011040-31011059	MS.gene066500:intron	15.0%
!!	ACAAATAAAGAGATATAAAG+AGG	-	chr8.2:31011282-31011301	None:intergenic	20.0%
!!	CAAATAAAAAGTTAGCTAAA+AGG	-	chr8.2:31010780-31010799	None:intergenic	20.0%
!!	TATTAGAAAAGATATATCAG+AGG	+	chr8.2:31011676-31011695	MS.gene066500:CDS	20.0%
!!	TCTATATATTCCAAAACATA+TGG	-	chr8.2:31010496-31010515	None:intergenic	20.0%
!!!	TCTTAATTTATCTTCAAGTT+TGG	-	chr8.2:31011612-31011631	None:intergenic	20.0%
!	AAGTGTATTCAAAATTGACA+AGG	-	chr8.2:31011885-31011904	None:intergenic	25.0%
!	CAAAAAAAAAAAACGAGACA+TGG	-	chr8.2:31011102-31011121	None:intergenic	25.0%
!	CTTAATTATAAGGATGAGAA+AGG	+	chr8.2:31010952-31010971	MS.gene066500:CDS	25.0%
!	TAGTAATATACCCTTGATTT+CGG	-	chr8.2:31011549-31011568	None:intergenic	25.0%
!	TGTCAAAAGATATATTGTCA+AGG	-	chr8.2:31011924-31011943	None:intergenic	25.0%
!	TTTCAATCTCATAATTTCAG+TGG	-	chr8.2:31010581-31010600	None:intergenic	25.0%
!!	AAGATCAAATGTCACATTTT+TGG	+	chr8.2:31011628-31011647	MS.gene066500:CDS	25.0%
!!	ATAACAACTATTTTATCCAC+AGG	+	chr8.2:31011439-31011458	MS.gene066500:intron	25.0%
!!	TGATATTGATTACAAAGTGA+AGG	+	chr8.2:31010417-31010436	MS.gene066500:CDS	25.0%
!!!	CATGTCTCGTTTTTTTTTTT+TGG	+	chr8.2:31011100-31011119	MS.gene066500:intron	25.0%
!!!	TCTCGTTTTTTTTTTTTGGA+GGG	+	chr8.2:31011104-31011123	MS.gene066500:intron	25.0%
	AAAAAAAAAACGAGACATGG+CGG	-	chr8.2:31011099-31011118	None:intergenic	30.0%
	AAGAGGACATGTTAGAAAAA+TGG	-	chr8.2:31011265-31011284	None:intergenic	30.0%
	AGCACTAAACAATGACTTAA+AGG	+	chr8.2:31010996-31011015	MS.gene066500:CDS	30.0%
	CATGTGACATCACAAATTAT+AGG	-	chr8.2:31011305-31011324	None:intergenic	30.0%
	CTTTGCATTCAAATTTACCT+TGG	-	chr8.2:31011486-31011505	None:intergenic	30.0%
	GATTGAAATCTTCATTTGCT+TGG	+	chr8.2:31010593-31010612	MS.gene066500:CDS	30.0%
	GCTTGCAAAGATTTAGTTAT+CGG	+	chr8.2:31011401-31011420	MS.gene066500:intron	30.0%
	TCATAATTTCAGTGGCAAAT+TGG	-	chr8.2:31010573-31010592	None:intergenic	30.0%
	TTAGAAAAATGGACCTTCTT+AGG	-	chr8.2:31011254-31011273	None:intergenic	30.0%
	TTCCTTAGAAATCAAAGAAG+TGG	-	chr8.2:31011825-31011844	None:intergenic	30.0%
!	ACTTGTTAGAATCAAAGGAA+GGG	+	chr8.2:31010708-31010727	MS.gene066500:CDS	30.0%
!	ATATATCTTTTGACACCAAG+TGG	+	chr8.2:31011929-31011948	MS.gene066500:intron	30.0%
!	TATATCTTTTGACACCAAGT+GGG	+	chr8.2:31011930-31011949	MS.gene066500:intron	30.0%
!	TCTTTGATTTCTAAGGAAGT+TGG	+	chr8.2:31011827-31011846	MS.gene066500:CDS	30.0%
!!	AACTTGTTAGAATCAAAGGA+AGG	+	chr8.2:31010707-31010726	MS.gene066500:CDS	30.0%
!!	AAGGAACTTGTTAGAATCAA+AGG	+	chr8.2:31010703-31010722	MS.gene066500:CDS	30.0%
!!	GGTAAATCAGTTATTTCTGA+AGG	+	chr8.2:31011848-31011867	MS.gene066500:CDS	30.0%
!!!	GTCTCGTTTTTTTTTTTTGG+AGG	+	chr8.2:31011103-31011122	MS.gene066500:intron	30.0%
	AAAACTAGGACAATATGCGT+TGG	+	chr8.2:31011205-31011224	MS.gene066500:intron	35.0%
	ATGGTATGATTGAACCAAGA+TGG	+	chr8.2:31011059-31011078	MS.gene066500:intron	35.0%
	ATTGAACCAAGATGGTGAAA+CGG	+	chr8.2:31011067-31011086	MS.gene066500:intron	35.0%
	ATTGATGCAGCTATATGTAG+AGG	-	chr8.2:31010541-31010560	None:intergenic	35.0%
	CCTCAATTAGTGTATAGAGA+AGG	-	chr8.2:31010468-31010487	None:intergenic	35.0%
	CCTTCTCTATACACTAATTG+AGG	+	chr8.2:31010465-31010484	MS.gene066500:CDS	35.0%
	CTACATATAGCTGCATCAAT+GGG	+	chr8.2:31010541-31010560	MS.gene066500:CDS	35.0%
	CTTGGAAGCTAAATCAACAA+GGG	+	chr8.2:31010611-31010630	MS.gene066500:CDS	35.0%
	GAGAATTTGAACTGACAGAT+AGG	-	chr8.2:31011170-31011189	None:intergenic	35.0%
	GCAAAACAACCAAAAGAGTA+TGG	+	chr8.2:31010657-31010676	MS.gene066500:CDS	35.0%
	GTAACATCTTCGATCGAATT+TGG	-	chr8.2:31010808-31010827	None:intergenic	35.0%
	TCGTTTCGTAGACATGAATA+AGG	+	chr8.2:31010684-31010703	MS.gene066500:CDS	35.0%
	TCTACATATAGCTGCATCAA+TGG	+	chr8.2:31010540-31010559	MS.gene066500:CDS	35.0%
	TCTCTATACACTAATTGAGG+AGG	+	chr8.2:31010468-31010487	MS.gene066500:CDS	35.0%
	TGTAGAGGAGTTTCAACAAA+TGG	-	chr8.2:31010526-31010545	None:intergenic	35.0%
!	AAGGGTATATTACTAAGTGC+TGG	+	chr8.2:31011554-31011573	MS.gene066500:CDS	35.0%
!	CACCACTTCTTTGATTTCTA+AGG	+	chr8.2:31011820-31011839	MS.gene066500:CDS	35.0%
!	TCAATCCATTTTGCCTAAGA+AGG	+	chr8.2:31011238-31011257	MS.gene066500:intron	35.0%
!	TCTTGTTTGTTAAGCGGTTT+AGG	+	chr8.2:31011135-31011154	MS.gene066500:intron	35.0%
!!	ACCATGTCTTGTTTGTTAAG+CGG	+	chr8.2:31011129-31011148	MS.gene066500:intron	35.0%
!!	ACTCTTTTGGTTGTTTTGCA+TGG	-	chr8.2:31010656-31010675	None:intergenic	35.0%
!!!	ATTGTCCTAGTTTTTTGACG+AGG	-	chr8.2:31011199-31011218	None:intergenic	35.0%
	ACCGCTTAACAAACAAGACA+TGG	-	chr8.2:31011133-31011152	None:intergenic	40.0%
	ATCGAAGATGTTACTCTGAG+GGG	+	chr8.2:31010814-31010833	MS.gene066500:CDS	40.0%
	ATGGACCTTCTTAGGCAAAA+TGG	-	chr8.2:31011246-31011265	None:intergenic	40.0%
	CCAAGCTCAAAAGTCAAAGA+TGG	+	chr8.2:31011584-31011603	MS.gene066500:CDS	40.0%
	CTTCATCTTGCTTGTCAAAG+TGG	+	chr8.2:31010745-31010764	MS.gene066500:CDS	40.0%
	CTTTGACAAGCAAGATGAAG+AGG	-	chr8.2:31010745-31010764	None:intergenic	40.0%
	GATCGAAGATGTTACTCTGA+GGG	+	chr8.2:31010813-31010832	MS.gene066500:CDS	40.0%
	GCTTGGAAGCTAAATCAACA+AGG	+	chr8.2:31010610-31010629	MS.gene066500:CDS	40.0%
	GGAGTTTATCAAGGTAATGC+AGG	+	chr8.2:31011776-31011795	MS.gene066500:CDS	40.0%
	GTTAGAATCAAAGGAAGGGA+AGG	+	chr8.2:31010712-31010731	MS.gene066500:CDS	40.0%
	TACCTTGATAAACTCCACCA+GGG	-	chr8.2:31011772-31011791	None:intergenic	40.0%
	TTACCTTGATAAACTCCACC+AGG	-	chr8.2:31011773-31011792	None:intergenic	40.0%
	TTGAACCAAGATGGTGAAAC+GGG	+	chr8.2:31011068-31011087	MS.gene066500:intron	40.0%
	TTTGTTAAGCGGTTTAGGTG+GGG	+	chr8.2:31011140-31011159	MS.gene066500:intron	40.0%
!	GTTTGAAGCTCTTCATGTCT+TGG	+	chr8.2:31010873-31010892	MS.gene066500:CDS	40.0%
!	GTTTGTTAAGCGGTTTAGGT+GGG	+	chr8.2:31011139-31011158	MS.gene066500:intron	40.0%
!	TGAGGAGGATCCATATGTTT+TGG	+	chr8.2:31010483-31010502	MS.gene066500:CDS	40.0%
!	TGTTTGTTAAGCGGTTTAGG+TGG	+	chr8.2:31011138-31011157	MS.gene066500:intron	40.0%
!!	AACGAAGCACCATACTCTTT+TGG	-	chr8.2:31010669-31010688	None:intergenic	40.0%
!!	CAAAATGGATTGAGCCTGAT+GGG	-	chr8.2:31011231-31011250	None:intergenic	40.0%
!!	CCATCTTTGACTTTTGAGCT+TGG	-	chr8.2:31011587-31011606	None:intergenic	40.0%
!!	TTTGGTTGTTTTGCATGGCA+AGG	-	chr8.2:31010651-31010670	None:intergenic	40.0%
	ACCTTGATAAACTCCACCAG+GGG	-	chr8.2:31011771-31011790	None:intergenic	45.0%
	ACTCGCCTCGTCAAAAAACT+AGG	+	chr8.2:31011191-31011210	MS.gene066500:intron	45.0%
	CGATCGAAGATGTTACTCTG+AGG	+	chr8.2:31010812-31010831	MS.gene066500:CDS	45.0%
	CTCTTCATGTCTTGGTTGGT+TGG	+	chr8.2:31010881-31010900	MS.gene066500:CDS	45.0%
	GAAGCTCTTCATGTCTTGGT+TGG	+	chr8.2:31010877-31010896	MS.gene066500:CDS	45.0%
	GTGAAGGTTGCAACTCTAGA+AGG	+	chr8.2:31010433-31010452	MS.gene066500:CDS	45.0%
	TTGAAGACAAATCGCCAGAG+AGG	+	chr8.2:31010904-31010923	MS.gene066500:CDS	45.0%
!	TCGACTGTTGGTAAAGACCA+AGG	+	chr8.2:31011466-31011485	MS.gene066500:CDS	45.0%
!	TTTTGCATGGCAAGGTGGAT+TGG	-	chr8.2:31010643-31010662	None:intergenic	45.0%
!!	AAAAACGAGACATGGCGGAT+AGG	-	chr8.2:31011094-31011113	None:intergenic	45.0%
!!	GCAAAATGGATTGAGCCTGA+TGG	-	chr8.2:31011232-31011251	None:intergenic	45.0%
	ATAGGCCCGTTTCACCATCT+TGG	-	chr8.2:31011076-31011095	None:intergenic	50.0%
	CCTTGATAAACTCCACCAGG+GGG	-	chr8.2:31011770-31011789	None:intergenic	50.0%
	TTAAGCGGTTTAGGTGGGGA+GGG	+	chr8.2:31011144-31011163	MS.gene066500:intron	50.0%
!	ACAATATGCGTTGGCCCATC+AGG	+	chr8.2:31011214-31011233	MS.gene066500:intron	50.0%
!!	AAATCGCCAGAGAGGTGCTA+GGG	+	chr8.2:31010912-31010931	MS.gene066500:CDS	50.0%
!!	AGCAGCAAGTGCCGAAATCA+AGG	+	chr8.2:31011535-31011554	MS.gene066500:CDS	50.0%
!!	GCAGCAAGTGCCGAAATCAA+GGG	+	chr8.2:31011536-31011555	MS.gene066500:CDS	50.0%
!!	GGTTGTTTTGCATGGCAAGG+TGG	-	chr8.2:31010648-31010667	None:intergenic	50.0%
	CCCCCTGGTGGAGTTTATCA+AGG	+	chr8.2:31011767-31011786	MS.gene066500:CDS	55.0%
	GTTAAGCGGTTTAGGTGGGG+AGG	+	chr8.2:31011143-31011162	MS.gene066500:intron	55.0%
!!	CAAATCGCCAGAGAGGTGCT+AGG	+	chr8.2:31010911-31010930	MS.gene066500:CDS	55.0%
	CCAACTCCCTAGCACCTCTC+TGG	-	chr8.2:31010921-31010940	None:intergenic	60.0%
	TCCACAGGCGCTTCGACTGT+TGG	+	chr8.2:31011454-31011473	MS.gene066500:intron	60.0%
!	ACCAACAGTCGAAGCGCCTG+TGG	-	chr8.2:31011458-31011477	None:intergenic	60.0%
!	CAATCAGCACTGAGTCCCCC+TGG	+	chr8.2:31011752-31011771	MS.gene066500:CDS	60.0%
!!	CCAGAGAGGTGCTAGGGAGT+TGG	+	chr8.2:31010918-31010937	MS.gene066500:CDS	60.0%
!	TCAGCACTGAGTCCCCCTGG+TGG	+	chr8.2:31011755-31011774	MS.gene066500:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	31010403	31011956	31010403	ID=MS.gene066500
chr8.2	mRNA	31010403	31011956	31010403	ID=MS.gene066500.t1;Parent=MS.gene066500
chr8.2	exon	31010403	31011017	31010403	ID=MS.gene066500.t1.exon1;Parent=MS.gene066500.t1
chr8.2	CDS	31010403	31011017	31010403	ID=cds.MS.gene066500.t1;Parent=MS.gene066500.t1
chr8.2	exon	31011461	31011869	31011461	ID=MS.gene066500.t1.exon2;Parent=MS.gene066500.t1
chr8.2	CDS	31011461	31011869	31011461	ID=cds.MS.gene066500.t1;Parent=MS.gene066500.t1
chr8.2	exon	31011949	31011956	31011949	ID=MS.gene066500.t1.exon3;Parent=MS.gene066500.t1
chr8.2	CDS	31011949	31011956	31011949	ID=cds.MS.gene066500.t1;Parent=MS.gene066500.t1

Gene Sequence

>MS.gene066500

ATGAACACAATGAATGATATTGATTACAAAGTGAAGGTTGCAACTCTAGAAGGTGATATAAACCTTCTCTATACACTAATTGAGGAGGATCCATATGTTTTGGAATATATAGATTTAATTCCATTTGTTGAAACTCCTCTACATATAGCTGCATCAATGGGACATGTCCAATTTGCCACTGAAATTATGAGATTGAAATCTTCATTTGCTTGGAAGCTAAATCAACAAGGGTTCAGTCCAATCCACCTTGCCATGCAAAACAACCAAAAGAGTATGGTGCTTCGTTTCGTAGACATGAATAAGGAACTTGTTAGAATCAAAGGAAGGGAAGGTTTAACTCCTCTTCATCTTGCTTGTCAAAGTGGAGAAATTGACCTTTTAGCTAACTTTTTATTTGTTTGTCCAAATTCGATCGAAGATGTTACTCTGAGGGGTGAGACTGCACTTCATATTTCTGTCAAAAATAAACAGTTTGAAGCTCTTCATGTCTTGGTTGGTTGGTTGAAGACAAATCGCCAGAGAGGTGCTAGGGAGTTGGAGAAATTAATACTTAATTATAAGGATGAGAAAGGAAACACTATTTTGCATATTTCAGCACTAAACAATGACTTAAAGGTAACCTTCTATTTAGTATATTTTATTTTTGATGAATAACTATGGTATGATTGAACCAAGATGGTGAAACGGGCCTATCCGCCATGTCTCGTTTTTTTTTTTTGGAGGGAAACCATGTCTTGTTTGTTAAGCGGTTTAGGTGGGGAGGGCCTATCTGTCAGTTCAAATTCTCAACTCGCCTCGTCAAAAAACTAGGACAATATGCGTTGGCCCATCAGGCTCAATCCATTTTGCCTAAGAAGGTCCATTTTTCTAACATGTCCTCTTTATATCTCTTTATTTGTCCTATAATTTGTGATGTCACATGAAAGAAAAGACATAACAGATATGTTAGTTCTAAATTGACGCTGAGATGCTGTTACAGTCTTTTAAAACTATTACTTGCTTGCAAAGATTTAGTTATCGGTTAAAGAAATGATTGATAACAACTATTTTATCCACAGGCGCTTCGACTGTTGGTAAAGACCAAGGTAAATTTGAATGCAAAGAATTCAGAGAACTCAACAGCATTAGACTTAGCAGCAAGTGCCGAAATCAAGGGTATATTACTAAGTGCTGGAGCAAAACCAAGCTCAAAAGTCAAAGATGGCTCCAAACTTGAAGATAAATTAAGATCAAATGTCACATTTTTGGATAAAATGTTAATTTATATACTGCGTATTAGAAAAGATATATCAGAGGAACAACGCAACGCTTTTCTAATAGTTGCGACTCTTATTGCAACTGCTACATATCAATCAGCACTGAGTCCCCCTGGTGGAGTTTATCAAGGTAATGCAGGAGACAATAACAATGTGAAAAACACCACTTCTTTGATTTCTAAGGAAGTTGGTAAATCAGTTATTTCTGAAGGTGATTTTTTTACCTTGTCAATTTTGAATACACTTTCACTTCTGTTATCAACCTTGACAATATATCTTTTGACACCAAGTGGGTTAA

Protein sequence

>MS.gene066500.t1

MNTMNDIDYKVKVATLEGDINLLYTLIEEDPYVLEYIDLIPFVETPLHIAASMGHVQFATEIMRLKSSFAWKLNQQGFSPIHLAMQNNQKSMVLRFVDMNKELVRIKGREGLTPLHLACQSGEIDLLANFLFVCPNSIEDVTLRGETALHISVKNKQFEALHVLVGWLKTNRQRGARELEKLILNYKDEKGNTILHISALNNDLKALRLLVKTKVNLNAKNSENSTALDLAASAEIKGILLSAGAKPSSKVKDGSKLEDKLRSNVTFLDKMLIYILRIRKDISEEQRNAFLIVATLIATATYQSALSPPGGVYQGNAGDNNNVKNTTSLISKEVGKSVISEVG