Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060740.t1 | XP_003602607.1 | 85.5 | 242 | 28 | 1 | 228 | 469 | 47 | 281 | 1.50E-106 | 396.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060740.t1 | O35130 | 51.2 | 205 | 96 | 2 | 265 | 469 | 43 | 243 | 3.4e-51 | 204.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060740.t1 | G7JBJ8 | 85.5 | 242 | 28 | 1 | 228 | 469 | 47 | 281 | 1.1e-106 | 396.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene012655 | MS.gene060740 | PPI |
| MS.gene060740 | MS.gene82426 | PPI |
| MS.gene060740 | MS.gene26077 | PPI |
| MS.gene060740 | MS.gene028153 | PPI |
| MS.gene060740 | MS.gene89206 | PPI |
| MS.gene060740 | MS.gene015586 | PPI |
| MS.gene060740 | MS.gene019309 | PPI |
| MS.gene063209 | MS.gene060740 | PPI |
| MS.gene060740 | MS.gene042172 | PPI |
| MS.gene060740 | MS.gene063568 | PPI |
| MS.gene060740 | MS.gene68942 | PPI |
| MS.gene060740 | MS.gene83444 | PPI |
| MS.gene060740 | MS.gene52173 | PPI |
| MS.gene42236 | MS.gene060740 | PPI |
| MS.gene060740 | MS.gene50744 | PPI |
| MS.gene060740 | MS.gene072782 | PPI |
| MS.gene27648 | MS.gene060740 | PPI |
| MS.gene060740 | MS.gene29448 | PPI |
| MS.gene060740 | MS.gene053381 | PPI |
| MS.gene060740 | MS.gene029825 | PPI |
| MS.gene060740 | MS.gene003693 | PPI |
| MS.gene060740 | MS.gene023799 | PPI |
| MS.gene060740 | MS.gene72768 | PPI |
| MS.gene75013 | MS.gene060740 | PPI |
| MS.gene59369 | MS.gene060740 | PPI |
| MS.gene060740 | MS.gene65479 | PPI |
| MS.gene017434 | MS.gene060740 | PPI |
| MS.gene060740 | MS.gene23314 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060740.t1 | MTR_3g096190 | 90.083 | 242 | 17 | 2 | 228 | 469 | 47 | 281 | 2.48e-154 | 440 |
| MS.gene060740.t1 | MTR_3g096190 | 82.540 | 252 | 35 | 3 | 4 | 252 | 18 | 263 | 1.38e-135 | 392 |
| MS.gene060740.t1 | MTR_4g032390 | 89.669 | 242 | 23 | 2 | 228 | 469 | 44 | 283 | 3.14e-153 | 437 |
| MS.gene060740.t1 | MTR_4g032390 | 87.054 | 224 | 27 | 2 | 4 | 227 | 18 | 239 | 1.36e-130 | 379 |
| MS.gene060740.t1 | MTR_3g096190 | 88.832 | 197 | 14 | 2 | 4 | 198 | 18 | 208 | 9.81e-114 | 334 |
| MS.gene060740.t1 | MTR_3g096190 | 89.349 | 169 | 11 | 2 | 228 | 396 | 47 | 208 | 4.06e-100 | 299 |
| MS.gene060740.t1 | MTR_5g464770 | 49.091 | 220 | 102 | 3 | 260 | 469 | 19 | 238 | 1.17e-63 | 206 |
| MS.gene060740.t1 | MTR_5g464770 | 50.588 | 170 | 82 | 2 | 62 | 229 | 19 | 188 | 1.22e-48 | 167 |
| MS.gene060740.t1 | MTR_1g112880 | 52.071 | 169 | 80 | 1 | 301 | 469 | 2 | 169 | 3.56e-55 | 182 |
| MS.gene060740.t1 | MTR_1g112880 | 46.980 | 149 | 77 | 2 | 103 | 250 | 2 | 149 | 8.13e-38 | 136 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060740.t1 | AT3G57000 | 63.525 | 244 | 84 | 4 | 228 | 469 | 57 | 297 | 8.58e-106 | 317 |
| MS.gene060740.t1 | AT3G57000 | 57.676 | 241 | 84 | 5 | 3 | 227 | 15 | 253 | 4.38e-86 | 266 |
Find 91 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGAACCGTTTGAATGTTCT+TGG | 0.244591 | 4.4:+71499080 | None:intergenic |
| GCGAACCGTTTGAATGTTCT+TGG | 0.244591 | 4.4:+71505127 | None:intergenic |
| GAGAGTTGACGGGCAAATAC+TGG | 0.282558 | 4.4:+71498832 | None:intergenic |
| GAGAGTTGACGGGCAAATAC+TGG | 0.282558 | 4.4:+71504888 | None:intergenic |
| TTCTGAAGATTTGGTTTCTT+AGG | 0.304076 | 4.4:+71507694 | None:intergenic |
| GCAAGAATCCTGGCGATTAC+AGG | 0.312927 | 4.4:-71506496 | MS.gene060740:CDS |
| TACAGTAAGCAGCACTTAAT+TGG | 0.314750 | 4.4:+71497902 | None:intergenic |
| TGTTCCTCGTTCTGAAGATT+TGG | 0.333064 | 4.4:+71507685 | None:intergenic |
| GCAAGGATCCTGGTCATTAC+AGG | 0.339703 | 4.4:-71500347 | MS.gene060740:CDS |
| ACGGGCAAATACTGGGTTAC+GGG | 0.353988 | 4.4:+71498840 | None:intergenic |
| ACGGGCAAATACTGGGTTAC+GGG | 0.353988 | 4.4:+71504896 | None:intergenic |
| ACCTCCCAGCTTTATTTAGT+TGG | 0.354972 | 4.4:+71499168 | None:intergenic |
| TAAAGCTGGGAGGTTGAAAA+TGG | 0.355073 | 4.4:-71499159 | MS.gene060740:CDS |
| TAAAGCTGGGAGGTTGAAAA+TGG | 0.355073 | 4.4:-71505206 | MS.gene060740:CDS |
| CAGGATTCTTGCTATGTTTG+AGG | 0.362731 | 4.4:+71506507 | None:intergenic |
| CAATCAGGACCTTGTCTTTG+TGG | 0.379821 | 4.4:-71498455 | MS.gene060740:intron |
| CAATCAGGACCTTGTCTTTG+TGG | 0.379821 | 4.4:-71504408 | MS.gene060740:intron |
| GTAGTTTCTGTTGGCGCACT+TGG | 0.393876 | 4.4:+71502406 | None:intergenic |
| GTCAACAATTCCAAGCAATC+AGG | 0.407962 | 4.4:-71498470 | MS.gene060740:CDS |
| GTCAACAATTCCAAGCAATC+AGG | 0.407962 | 4.4:-71504423 | MS.gene060740:CDS |
| TCATTCAAAGGAGGAGCAAT+TGG | 0.409680 | 4.4:+71502349 | None:intergenic |
| CAGGCCTGACATTTGTCATC+AGG | 0.410963 | 4.4:-71500328 | MS.gene060740:intron |
| CAGGCCTGACATTTGTCATC+AGG | 0.410963 | 4.4:-71506477 | MS.gene060740:intron |
| GTATACATAAGGACCGAAAA+AGG | 0.411155 | 4.4:-71499137 | MS.gene060740:CDS |
| GTATACATAAGGACTGAAAA+AGG | 0.415050 | 4.4:-71505184 | MS.gene060740:CDS |
| GACAGCCAACTAAATAAAGC+TGG | 0.432597 | 4.4:-71499173 | MS.gene060740:CDS |
| AAGGATTACTAATGCTATTG+AGG | 0.435293 | 4.4:-71497875 | MS.gene060740:CDS |
| TCTTTAGAAGTCGCTAAAGT+TGG | 0.449255 | 4.4:-71502280 | MS.gene060740:CDS |
| TCTTTAGAAGTCGCTAAAGT+TGG | 0.449255 | 4.4:-71507541 | MS.gene060740:CDS |
| ACCGATGTAACTGAACTTCC+TGG | 0.451025 | 4.4:-71502376 | MS.gene060740:CDS |
| AGATTTGGTTTCTTAGGTGT+TGG | 0.452992 | 4.4:+71507700 | None:intergenic |
| TACATAGATGATTATATAGC+TGG | 0.453978 | 4.4:-71498318 | MS.gene060740:intron |
| ATCTTATAGTTCAGAGAAGC+TGG | 0.455879 | 4.4:-71498512 | MS.gene060740:CDS |
| ATCTTATAGTTCAGAGAAGC+TGG | 0.455879 | 4.4:-71504465 | MS.gene060740:CDS |
| CAGGATCCTTGCTATGTTTG+CGG | 0.457691 | 4.4:+71500358 | None:intergenic |
| GATAGCACACTAAATAAAGC+TGG | 0.459423 | 4.4:-71505220 | MS.gene060740:CDS |
| AGGATTACTAATGCTATTGA+GGG | 0.461127 | 4.4:-71497874 | MS.gene060740:CDS |
| CCCGTCAACTCTCGAAAAGC+AGG | 0.471030 | 4.4:-71498822 | MS.gene060740:intron |
| CCCGTCAACTCTCGAAAAGC+AGG | 0.471030 | 4.4:-71504878 | MS.gene060740:intron |
| AAACTGAGTATAACTGCTGC+TGG | 0.476547 | 4.4:-71504953 | MS.gene060740:CDS |
| AAGACAAGGTCCTGATTGCT+TGG | 0.489499 | 4.4:+71498460 | None:intergenic |
| AAGACAAGGTCCTGATTGCT+TGG | 0.489499 | 4.4:+71504413 | None:intergenic |
| AAATTGAGTATAACTGCTGC+TGG | 0.491203 | 4.4:-71498897 | MS.gene060740:CDS |
| CTAATTTAAGGTTGGAGCAA+TGG | 0.500486 | 4.4:-71498364 | MS.gene060740:intron |
| ACCGACGAAACTGAACTTCC+TGG | 0.505847 | 4.4:-71507634 | MS.gene060740:CDS |
| GTTCTTGGAATAAGAACATA+GGG | 0.506707 | 4.4:+71499095 | None:intergenic |
| CTAATGCTATTGAGGGAAAG+TGG | 0.509443 | 4.4:-71497867 | MS.gene060740:CDS |
| GGTTGAAAATGGTATACATA+AGG | 0.511316 | 4.4:-71499148 | MS.gene060740:CDS |
| GGTTGAAAATGGTATACATA+AGG | 0.511316 | 4.4:-71505195 | MS.gene060740:CDS |
| CGCAAACATAGCAAGGATCC+TGG | 0.518066 | 4.4:-71500357 | MS.gene060740:CDS |
| CGGGCAAATACTGGGTTACG+GGG | 0.520947 | 4.4:+71498841 | None:intergenic |
| CGGGCAAATACTGGGTTACG+GGG | 0.520947 | 4.4:+71504897 | None:intergenic |
| GGAGGAGCAATTGGAATACC+AGG | 0.523622 | 4.4:+71502358 | None:intergenic |
| GACGGGCAAATACTGGGTTA+CGG | 0.523874 | 4.4:+71498839 | None:intergenic |
| GACGGGCAAATACTGGGTTA+CGG | 0.523874 | 4.4:+71504895 | None:intergenic |
| TGGAATTGTTGACAAATACT+TGG | 0.529015 | 4.4:+71498480 | None:intergenic |
| GTGCTGCTTACTGTATTACA+AGG | 0.530206 | 4.4:-71497894 | MS.gene060740:CDS |
| ACCAGGAAGTTCAGTTTCGT+CGG | 0.530441 | 4.4:+71507633 | None:intergenic |
| ATGTCAGGCCTGTAATCGCC+AGG | 0.533692 | 4.4:+71506488 | None:intergenic |
| TGTTCTTGGAATAAGAACAT+AGG | 0.541341 | 4.4:+71499094 | None:intergenic |
| AGAGTTGACGGGCAAATACT+GGG | 0.541627 | 4.4:+71498833 | None:intergenic |
| AGAGTTGACGGGCAAATACT+GGG | 0.541627 | 4.4:+71504889 | None:intergenic |
| AGAACATTCAAACGGTTCGC+TGG | 0.542174 | 4.4:-71499077 | MS.gene060740:CDS |
| AAGGTTGGAGCAATGGCACA+TGG | 0.543720 | 4.4:-71498357 | MS.gene060740:CDS |
| ATGTTCTTGGAATACGAACA+TGG | 0.550576 | 4.4:+71505140 | None:intergenic |
| AAAGGAGCAATTGGAATACC+AGG | 0.551218 | 4.4:+71507616 | None:intergenic |
| TTATTCCAAGAACATTCAAA+CGG | 0.551522 | 4.4:-71499085 | MS.gene060740:CDS |
| AGAACATTCAAACGGTTCGC+TGG | 0.554467 | 4.4:-71505124 | MS.gene060740:CDS |
| GTATTCCAAGAACATTCAAA+CGG | 0.559538 | 4.4:-71505132 | MS.gene060740:CDS |
| TGGAGCAATGGCACATGGGA+AGG | 0.561421 | 4.4:-71498352 | MS.gene060740:CDS |
| CTCAAACATAGCAAGAATCC+TGG | 0.561681 | 4.4:-71506506 | MS.gene060740:CDS |
| CCAACCTGATGACAAATGTC+AGG | 0.572185 | 4.4:+71500324 | None:intergenic |
| CCAACCTGATGACAAATGTC+AGG | 0.572185 | 4.4:+71506473 | None:intergenic |
| AGAAGTCGCTAAAGTTGGCA+AGG | 0.584499 | 4.4:-71502275 | MS.gene060740:intron |
| AGAAGTCGCTAAAGTTGGCA+AGG | 0.591319 | 4.4:-71507536 | MS.gene060740:intron |
| ACAGCCAACTAAATAAAGCT+GGG | 0.593260 | 4.4:-71499172 | MS.gene060740:CDS |
| TTTCCTCCGCAAACATAGCA+AGG | 0.593451 | 4.4:-71500364 | MS.gene060740:CDS |
| ATAGCACACTAAATAAAGCT+GGG | 0.597262 | 4.4:-71505219 | MS.gene060740:CDS |
| GATTCTTGCTATGTTTGAGG+AGG | 0.599323 | 4.4:+71506510 | None:intergenic |
| ACCAGGAAGTTCAGTTACAT+CGG | 0.602601 | 4.4:+71502375 | None:intergenic |
| ATGTCAGGCCTGTAATGACC+AGG | 0.605340 | 4.4:+71500339 | None:intergenic |
| TGTTCTTGGAATACGAACAT+GGG | 0.611478 | 4.4:+71505141 | None:intergenic |
| GATCCTTGCTATGTTTGCGG+AGG | 0.633807 | 4.4:+71500361 | None:intergenic |
| GTTCTTGGAATACGAACATG+GGG | 0.659189 | 4.4:+71505142 | None:intergenic |
| GAAAAGAATAGTGAGAAACA+AGG | 0.661309 | 4.4:-71507589 | MS.gene060740:CDS |
| AGGTTGGAGCAATGGCACAT+GGG | 0.675683 | 4.4:-71498356 | MS.gene060740:CDS |
| GAAACCAAATCTTCAGAACG+AGG | 0.704893 | 4.4:-71507689 | MS.gene060740:CDS |
| TAGGGAGTACCACAAAGACA+AGG | 0.712278 | 4.4:+71498446 | None:intergenic |
| TAGGGAGTACCACAAAGACA+AGG | 0.712278 | 4.4:+71504399 | None:intergenic |
| GCCAACTAAATAAAGCTGGG+AGG | 0.728784 | 4.4:-71499169 | MS.gene060740:CDS |
| GCACACTAAATAAAGCTGGG+AGG | 0.737869 | 4.4:-71505216 | MS.gene060740:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 71497849 | 71507785 | 71497849 | ID=MS.gene060740 |
| chr4.4 | mRNA | 71497849 | 71507785 | 71497849 | ID=MS.gene060740.t1;Parent=MS.gene060740 |
| chr4.4 | exon | 71507537 | 71507785 | 71507537 | ID=MS.gene060740.t1.exon1;Parent=MS.gene060740.t1 |
| chr4.4 | CDS | 71507537 | 71507785 | 71507537 | ID=cds.MS.gene060740.t1;Parent=MS.gene060740.t1 |
| chr4.4 | exon | 71506478 | 71506570 | 71506478 | ID=MS.gene060740.t1.exon2;Parent=MS.gene060740.t1 |
| chr4.4 | CDS | 71506478 | 71506570 | 71506478 | ID=cds.MS.gene060740.t1;Parent=MS.gene060740.t1 |
| chr4.4 | exon | 71505116 | 71505260 | 71505116 | ID=MS.gene060740.t1.exon3;Parent=MS.gene060740.t1 |
| chr4.4 | CDS | 71505116 | 71505260 | 71505116 | ID=cds.MS.gene060740.t1;Parent=MS.gene060740.t1 |
| chr4.4 | exon | 71504879 | 71504989 | 71504879 | ID=MS.gene060740.t1.exon4;Parent=MS.gene060740.t1 |
| chr4.4 | CDS | 71504879 | 71504989 | 71504879 | ID=cds.MS.gene060740.t1;Parent=MS.gene060740.t1 |
| chr4.4 | exon | 71504409 | 71504491 | 71504409 | ID=MS.gene060740.t1.exon5;Parent=MS.gene060740.t1 |
| chr4.4 | CDS | 71504409 | 71504491 | 71504409 | ID=cds.MS.gene060740.t1;Parent=MS.gene060740.t1 |
| chr4.4 | exon | 71502276 | 71502437 | 71502276 | ID=MS.gene060740.t1.exon6;Parent=MS.gene060740.t1 |
| chr4.4 | CDS | 71502276 | 71502437 | 71502276 | ID=cds.MS.gene060740.t1;Parent=MS.gene060740.t1 |
| chr4.4 | exon | 71500329 | 71500421 | 71500329 | ID=MS.gene060740.t1.exon7;Parent=MS.gene060740.t1 |
| chr4.4 | CDS | 71500329 | 71500421 | 71500329 | ID=cds.MS.gene060740.t1;Parent=MS.gene060740.t1 |
| chr4.4 | exon | 71499069 | 71499213 | 71499069 | ID=MS.gene060740.t1.exon8;Parent=MS.gene060740.t1 |
| chr4.4 | CDS | 71499069 | 71499213 | 71499069 | ID=cds.MS.gene060740.t1;Parent=MS.gene060740.t1 |
| chr4.4 | exon | 71498823 | 71498933 | 71498823 | ID=MS.gene060740.t1.exon9;Parent=MS.gene060740.t1 |
| chr4.4 | CDS | 71498823 | 71498933 | 71498823 | ID=cds.MS.gene060740.t1;Parent=MS.gene060740.t1 |
| chr4.4 | exon | 71498456 | 71498538 | 71498456 | ID=MS.gene060740.t1.exon10;Parent=MS.gene060740.t1 |
| chr4.4 | CDS | 71498456 | 71498538 | 71498456 | ID=cds.MS.gene060740.t1;Parent=MS.gene060740.t1 |
| chr4.4 | exon | 71498319 | 71498376 | 71498319 | ID=MS.gene060740.t1.exon11;Parent=MS.gene060740.t1 |
| chr4.4 | CDS | 71498319 | 71498376 | 71498319 | ID=cds.MS.gene060740.t1;Parent=MS.gene060740.t1 |
| chr4.4 | exon | 71497849 | 71497934 | 71497849 | ID=MS.gene060740.t1.exon12;Parent=MS.gene060740.t1 |
| chr4.4 | CDS | 71497849 | 71497934 | 71497849 | ID=cds.MS.gene060740.t1;Parent=MS.gene060740.t1 |
| Gene Sequence |
| Protein sequence |
