Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29448.t1 | XP_024640360.1 | 91.3 | 620 | 26 | 2 | 1 | 601 | 1 | 611 | 1.20E-291 | 1011.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29448.t1 | Q8BHY2 | 40.7 | 280 | 135 | 4 | 268 | 546 | 246 | 495 | 1.4e-49 | 199.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29448.t1 | G7K1U1 | 90.6 | 620 | 26 | 2 | 1 | 601 | 1 | 607 | 4.1e-289 | 1003.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29448.t1 | MTR_5g078780 | 90.050 | 603 | 28 | 2 | 18 | 601 | 18 | 607 | 0.0 | 1071 |
| MS.gene29448.t1 | MTR_5g078810 | 75.714 | 70 | 17 | 0 | 355 | 424 | 1 | 70 | 3.49e-26 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29448.t1 | AT2G17250 | 55.766 | 581 | 226 | 6 | 12 | 573 | 8 | 576 | 0.0 | 641 |
Find 135 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACATTATTGTTACGTTTCT+TGG | 0.096967 | 5.3:-64039888 | None:intergenic |
| AATGGTCAAGGCCTGATTTC+TGG | 0.177335 | 5.3:-64044462 | None:intergenic |
| TTACTAACCTTGAGCTTAAT+TGG | 0.193248 | 5.3:-64038923 | None:intergenic |
| TGAAAAGCTCACTAGAAATT+TGG | 0.202782 | 5.3:+64038706 | MS.gene29448:CDS |
| CTTCATCTTCTTCGCAATTC+TGG | 0.241906 | 5.3:-64040124 | None:intergenic |
| TTGGAGGGAAAAGATGTTTC+AGG | 0.244460 | 5.3:+64038725 | MS.gene29448:CDS |
| TTGCTTTCAGTTCCACCTTC+TGG | 0.244476 | 5.3:+64042876 | MS.gene29448:CDS |
| CTTGAGCAGGAAGCTCTCTT+TGG | 0.258522 | 5.3:+64044942 | MS.gene29448:intron |
| GAACAGGGCTAAAGATTTAT+TGG | 0.262375 | 5.3:+64045752 | MS.gene29448:CDS |
| TTTAGGTTTGCTTTGTCTCT+TGG | 0.268396 | 5.3:+64045128 | MS.gene29448:intron |
| CAGATTCCTCAATGTTAAAA+TGG | 0.289979 | 5.3:-64044480 | None:intergenic |
| AAGCTTGGATTGCGTATCTT+AGG | 0.300738 | 5.3:+64040160 | MS.gene29448:CDS |
| CCAATCCATGCTGGGTCATT+AGG | 0.302737 | 5.3:-64041694 | None:intergenic |
| TTAGGTTTGCTTTGTCTCTT+GGG | 0.321498 | 5.3:+64045129 | MS.gene29448:intron |
| ATTAGTTCCATCTATCTTCA+TGG | 0.328121 | 5.3:+64041751 | MS.gene29448:CDS |
| GGTTCTTATGCAACAATACT+TGG | 0.336089 | 5.3:+64045209 | MS.gene29448:CDS |
| TGAATGCGATGAAACTATTA+AGG | 0.339514 | 5.3:+64038009 | MS.gene29448:CDS |
| GAAGGGAGTTGAGGGAGGAG+AGG | 0.348058 | 5.3:-64037863 | None:intergenic |
| GTGGTGGTTGCGGTGGCGGA+AGG | 0.353194 | 5.3:-64037881 | None:intergenic |
| ATGTGGTGAGGTGGAGAAGA+AGG | 0.362280 | 5.3:-64037803 | None:intergenic |
| GCTGGTGGTGGTTGCGGTGG+CGG | 0.371522 | 5.3:-64037885 | None:intergenic |
| TCAGTGATGCTGTGTTGTTT+TGG | 0.385668 | 5.3:-64037701 | None:intergenic |
| TTGAGCAGGAAGCTCTCTTT+GGG | 0.386720 | 5.3:+64044943 | MS.gene29448:intron |
| ACCCCTTCTTCCAGCTTACC+TGG | 0.387473 | 5.3:+64042821 | MS.gene29448:CDS |
| TGCATTGGTTATTACAGCTC+TGG | 0.389369 | 5.3:+64042899 | MS.gene29448:CDS |
| AAAACAAGCGAAGTTAATAT+TGG | 0.391828 | 5.3:+64045173 | MS.gene29448:CDS |
| CTGGAAGAAGGGGTGACTTT+AGG | 0.399170 | 5.3:-64042812 | None:intergenic |
| TGTGGTGAGGTGGAGAAGAA+GGG | 0.400686 | 5.3:-64037802 | None:intergenic |
| CGAAGAGACTGCTTCTAACT+TGG | 0.405411 | 5.3:+64044411 | MS.gene29448:CDS |
| GAGATGAATGTGAGGAGGAG+AGG | 0.410717 | 5.3:-64037779 | None:intergenic |
| AGATGAATGTGAGGAGGAGA+GGG | 0.412733 | 5.3:-64037778 | None:intergenic |
| AAGGGAGTTGAGGGAGGAGA+GGG | 0.413318 | 5.3:-64037862 | None:intergenic |
| TCATCGACAATAATGAGAAC+AGG | 0.414010 | 5.3:+64045736 | MS.gene29448:CDS |
| CGATCAACTGTTTGGTGCAC+CGG | 0.416098 | 5.3:+64042949 | MS.gene29448:CDS |
| GCAAAGCTCTGCCAGAAATC+AGG | 0.417403 | 5.3:+64044451 | MS.gene29448:CDS |
| TTAGAAATGTGGAGTGTATC+AGG | 0.418848 | 5.3:+64039387 | MS.gene29448:CDS |
| CCACGTGAGGTAAATGAATT+CGG | 0.420969 | 5.3:-64037930 | None:intergenic |
| GGAGGAGCTGGTGGTGGTTG+CGG | 0.422441 | 5.3:-64037891 | None:intergenic |
| TCTTAGGTTGCCACTTCCAT+TGG | 0.431065 | 5.3:+64040176 | MS.gene29448:CDS |
| TACTTTGATGGAGTTTGTTA+AGG | 0.432512 | 5.3:+64038045 | MS.gene29448:CDS |
| AGTTGTGCTTGATACTTTGA+TGG | 0.436883 | 5.3:+64038033 | MS.gene29448:CDS |
| AGTTCCACCTTCTGGTGCAT+TGG | 0.437968 | 5.3:+64042884 | MS.gene29448:CDS |
| ACATCCTTCGATCAACTGTT+TGG | 0.439932 | 5.3:+64042941 | MS.gene29448:CDS |
| TGTCTAATCCCATCATGTTA+TGG | 0.440426 | 5.3:+64041522 | MS.gene29448:CDS |
| AGGGAGGAGAGGGAGGAAGA+AGG | 0.440748 | 5.3:-64037852 | None:intergenic |
| GGAGTTGAGGGAGGAGAGGG+AGG | 0.440858 | 5.3:-64037859 | None:intergenic |
| AGTTAAAATTTACTAAAGCT+TGG | 0.442017 | 5.3:+64040145 | MS.gene29448:CDS |
| CTCATAGCGCCTGACTTCAT+AGG | 0.448545 | 5.3:-64044505 | None:intergenic |
| GCTGGGTCATTAGGACATAA+AGG | 0.449537 | 5.3:-64041685 | None:intergenic |
| AATGAGGCAGCCAGGTAAGC+TGG | 0.457951 | 5.3:-64042831 | None:intergenic |
| TGGTGGTTGCGGTGGCGGAA+GGG | 0.462845 | 5.3:-64037880 | None:intergenic |
| CGGTGGCGGAAGGGAGTTGA+GGG | 0.464726 | 5.3:-64037871 | None:intergenic |
| ACCACCACCAGCTCCTCCTC+TGG | 0.470797 | 5.3:+64037896 | MS.gene29448:CDS |
| TTTCTTCATTGCAGGTTCCA+TGG | 0.470904 | 5.3:-64037654 | None:intergenic |
| TTGGGTATTCCAATCCATGC+TGG | 0.473646 | 5.3:-64041703 | None:intergenic |
| ATTTCAAGTACATTGATGTT+CGG | 0.476121 | 5.3:+64038295 | MS.gene29448:CDS |
| GTTGTTGATGTGTGAGGCGG+AGG | 0.482954 | 5.3:-64037753 | None:intergenic |
| CAGCCAGGTAAGCTGGAAGA+AGG | 0.488496 | 5.3:-64042824 | None:intergenic |
| AGCCAGGTAAGCTGGAAGAA+GGG | 0.489279 | 5.3:-64042823 | None:intergenic |
| CCTAATGACCCAGCATGGAT+TGG | 0.489583 | 5.3:+64041694 | MS.gene29448:CDS |
| ACTCAATTTCTTTGCAAATG+AGG | 0.492729 | 5.3:-64042847 | None:intergenic |
| CAAAACACTTACCGTGAGAC+AGG | 0.494028 | 5.3:-64044998 | None:intergenic |
| GAAGAGAGAGAATCGATTCG+AGG | 0.495834 | 5.3:-64037826 | None:intergenic |
| GGAGAGAAGTTCTTGACCTA+AGG | 0.496473 | 5.3:-64037732 | None:intergenic |
| TGAGCTTAATTGGCTTTCAT+CGG | 0.499732 | 5.3:-64038913 | None:intergenic |
| GTGGTGAGGTGGAGAAGAAG+GGG | 0.502021 | 5.3:-64037801 | None:intergenic |
| TGAATGTGAGGAGGAGAGGG+AGG | 0.502763 | 5.3:-64037775 | None:intergenic |
| ATCACTGAAATCAAAACCTT+AGG | 0.502878 | 5.3:+64037716 | MS.gene29448:CDS |
| GAATCTGATCCTATGAAGTC+AGG | 0.505902 | 5.3:+64044496 | MS.gene29448:CDS |
| TTGACAAGGTCGTATGATGT+AGG | 0.506754 | 5.3:+64041632 | MS.gene29448:CDS |
| TTCTCTGAAAAGAGAGAGGA+TGG | 0.507254 | 5.3:-64045666 | None:intergenic |
| GTAAATGAATTCGGATTGAT+CGG | 0.509695 | 5.3:-64037921 | None:intergenic |
| TCTTTGCAAATGAGGCAGCC+AGG | 0.517329 | 5.3:-64042839 | None:intergenic |
| AAAGCTCACTAGAAATTTGG+AGG | 0.519018 | 5.3:+64038709 | MS.gene29448:CDS |
| ATCGGAACCAGAGGAGGAGC+TGG | 0.519725 | 5.3:-64037903 | None:intergenic |
| GGAGCTGGTGGTGGTTGCGG+TGG | 0.521533 | 5.3:-64037888 | None:intergenic |
| TGGGTATTCCAATCCATGCT+GGG | 0.522547 | 5.3:-64041702 | None:intergenic |
| AGAGAATCGATTCGAGGATG+TGG | 0.533005 | 5.3:-64037820 | None:intergenic |
| ACCAGAGGAGGAGCTGGTGG+TGG | 0.535853 | 5.3:-64037897 | None:intergenic |
| AAGAAGGGGAGATGAATGTG+AGG | 0.536341 | 5.3:-64037787 | None:intergenic |
| CCGAATTCATTTACCTCACG+TGG | 0.537243 | 5.3:+64037930 | MS.gene29448:CDS |
| GCGGTGGCGGAAGGGAGTTG+AGG | 0.538128 | 5.3:-64037872 | None:intergenic |
| GATAAAACTGCAAGCGCAGA+TGG | 0.542599 | 5.3:+64038888 | MS.gene29448:CDS |
| ATCACTTATCGATTGTCTTG+TGG | 0.543229 | 5.3:+64037982 | MS.gene29448:CDS |
| GAGGAATCACTGCTTGGTGA+AGG | 0.547446 | 5.3:-64041496 | None:intergenic |
| ACCTCTTTGAACACATCCAA+TGG | 0.549353 | 5.3:-64040192 | None:intergenic |
| GATCAACTGTTTGGTGCACC+GGG | 0.550891 | 5.3:+64042950 | MS.gene29448:CDS |
| TTGAACACATCCAATGGAAG+TGG | 0.555934 | 5.3:-64040186 | None:intergenic |
| GATTACACGGAGGATGAAGC+AGG | 0.556176 | 5.3:+64045617 | MS.gene29448:intron |
| GCCAGGTAAGCTGGAAGAAG+GGG | 0.560855 | 5.3:-64042822 | None:intergenic |
| GGCTAAAGATTTATTGGACC+AGG | 0.561337 | 5.3:+64045758 | MS.gene29448:CDS |
| AGGTGTTTACCATAACATGA+TGG | 0.563701 | 5.3:-64041531 | None:intergenic |
| TCCATTGGATGTGTTCAAAG+AGG | 0.564996 | 5.3:+64040191 | MS.gene29448:CDS |
| GGTGTTTACCATAACATGAT+GGG | 0.565453 | 5.3:-64041530 | None:intergenic |
| ATATGGTGTAGTACATATTG+TGG | 0.566500 | 5.3:-64039323 | None:intergenic |
| AAGGGGAGATGAATGTGAGG+AGG | 0.575380 | 5.3:-64037784 | None:intergenic |
| AGGGAGGTTGTTGATGTGTG+AGG | 0.576196 | 5.3:-64037759 | None:intergenic |
| TTGCACCATTGCAGATTACA+CGG | 0.577570 | 5.3:+64045604 | MS.gene29448:intron |
| ATGAAAGCCAATTAAGCTCA+AGG | 0.578137 | 5.3:+64038916 | MS.gene29448:CDS |
| TGGCGGAAGGGAGTTGAGGG+AGG | 0.579360 | 5.3:-64037868 | None:intergenic |
| CCTCAATGTTAAAATGGTCA+AGG | 0.580409 | 5.3:-64044474 | None:intergenic |
| TAAAATGCTTTCTGTAGATG+TGG | 0.586404 | 5.3:+64039841 | MS.gene29448:CDS |
| AACTTGGATAATGCTCAGAA+TGG | 0.586561 | 5.3:+64044427 | MS.gene29448:CDS |
| AGACAGGAGGACAGTAGTGG+TGG | 0.591867 | 5.3:-64044982 | None:intergenic |
| ACAGATGAGGAATCACTGCT+TGG | 0.595439 | 5.3:-64041502 | None:intergenic |
| GATTCGAGGATGTGGTGAGG+TGG | 0.595441 | 5.3:-64037812 | None:intergenic |
| TCATGGCAAAACATCGTGCA+AGG | 0.595702 | 5.3:+64041768 | MS.gene29448:CDS |
| TTATGCAACAATACTTGGAG+CGG | 0.603488 | 5.3:+64045214 | MS.gene29448:CDS |
| TTCGGATTGATCGGAACCAG+AGG | 0.606360 | 5.3:-64037912 | None:intergenic |
| GAGGTTGTTGATGTGTGAGG+CGG | 0.608742 | 5.3:-64037756 | None:intergenic |
| CATCGACAATAATGAGAACA+GGG | 0.609216 | 5.3:+64045737 | MS.gene29448:CDS |
| TGCAACAATACTTGGAGCGG+AGG | 0.610817 | 5.3:+64045217 | MS.gene29448:CDS |
| ACTTATCGATTGTCTTGTGG+CGG | 0.611390 | 5.3:+64037985 | MS.gene29448:CDS |
| ATCATTCTCTGAAAAGAGAG+AGG | 0.615510 | 5.3:-64045670 | None:intergenic |
| ATAACCAATGCACCAGAAGG+TGG | 0.616650 | 5.3:-64042888 | None:intergenic |
| TGCACCAAACAGTTGATCGA+AGG | 0.618010 | 5.3:-64042945 | None:intergenic |
| CATATTGTGGATGATGCACT+CGG | 0.624795 | 5.3:-64039310 | None:intergenic |
| ATACATCTGATTTAGAAATG+TGG | 0.629117 | 5.3:+64039376 | MS.gene29448:CDS |
| TATGTCCTAATGACCCAGCA+TGG | 0.629145 | 5.3:+64041689 | MS.gene29448:CDS |
| ACTACTGTCCTCCTGTCTCA+CGG | 0.630327 | 5.3:+64044987 | MS.gene29448:CDS |
| GTAATAACCAATGCACCAGA+AGG | 0.635450 | 5.3:-64042891 | None:intergenic |
| AAGCTCACTAGAAATTTGGA+GGG | 0.638285 | 5.3:+64038710 | MS.gene29448:CDS |
| GGAACCAGAGGAGGAGCTGG+TGG | 0.642590 | 5.3:-64037900 | None:intergenic |
| ATCGATTCGAGGATGTGGTG+AGG | 0.646651 | 5.3:-64037815 | None:intergenic |
| GTGAGACAGGAGGACAGTAG+TGG | 0.650028 | 5.3:-64044985 | None:intergenic |
| CATTTGCAAAGAAATTGAGT+AGG | 0.655411 | 5.3:+64042850 | MS.gene29448:CDS |
| GAAACTCGTCGAACTTCGAA+CGG | 0.658319 | 5.3:-64037955 | None:intergenic |
| ACTTCGAACGGAGCCACGTG+AGG | 0.663920 | 5.3:-64037943 | None:intergenic |
| TGTGCAGTGATTTCTTGACA+AGG | 0.666059 | 5.3:+64041618 | MS.gene29448:intron |
| GGATTGATCGGAACCAGAGG+AGG | 0.668743 | 5.3:-64037909 | None:intergenic |
| AGGTGGTGTAGTCAGCGTGA+TGG | 0.671351 | 5.3:+64041652 | MS.gene29448:CDS |
| AACACTTACCGTGAGACAGG+AGG | 0.675774 | 5.3:-64044995 | None:intergenic |
| ACAAGGTCGTATGATGTAGG+TGG | 0.691770 | 5.3:+64041635 | MS.gene29448:CDS |
| ATGATGGGATTAGACAGATG+AGG | 0.697642 | 5.3:-64041515 | None:intergenic |
| CACCATTGCAGATTACACGG+AGG | 0.749176 | 5.3:+64045607 | MS.gene29448:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 64037656 | 64045821 | 64037656 | ID=MS.gene29448 |
| chr5.3 | mRNA | 64037656 | 64045821 | 64037656 | ID=MS.gene29448.t1;Parent=MS.gene29448 |
| chr5.3 | exon | 64037656 | 64038066 | 64037656 | ID=MS.gene29448.t1.exon1;Parent=MS.gene29448.t1 |
| chr5.3 | CDS | 64037656 | 64038066 | 64037656 | ID=cds.MS.gene29448.t1;Parent=MS.gene29448.t1 |
| chr5.3 | exon | 64038243 | 64038316 | 64038243 | ID=MS.gene29448.t1.exon2;Parent=MS.gene29448.t1 |
| chr5.3 | CDS | 64038243 | 64038316 | 64038243 | ID=cds.MS.gene29448.t1;Parent=MS.gene29448.t1 |
| chr5.3 | exon | 64038685 | 64038746 | 64038685 | ID=MS.gene29448.t1.exon3;Parent=MS.gene29448.t1 |
| chr5.3 | CDS | 64038685 | 64038746 | 64038685 | ID=cds.MS.gene29448.t1;Parent=MS.gene29448.t1 |
| chr5.3 | exon | 64038886 | 64038937 | 64038886 | ID=MS.gene29448.t1.exon4;Parent=MS.gene29448.t1 |
| chr5.3 | CDS | 64038886 | 64038937 | 64038886 | ID=cds.MS.gene29448.t1;Parent=MS.gene29448.t1 |
| chr5.3 | exon | 64039308 | 64039408 | 64039308 | ID=MS.gene29448.t1.exon5;Parent=MS.gene29448.t1 |
| chr5.3 | CDS | 64039308 | 64039408 | 64039308 | ID=cds.MS.gene29448.t1;Parent=MS.gene29448.t1 |
| chr5.3 | exon | 64039831 | 64039907 | 64039831 | ID=MS.gene29448.t1.exon6;Parent=MS.gene29448.t1 |
| chr5.3 | CDS | 64039831 | 64039907 | 64039831 | ID=cds.MS.gene29448.t1;Parent=MS.gene29448.t1 |
| chr5.3 | exon | 64040111 | 64040212 | 64040111 | ID=MS.gene29448.t1.exon7;Parent=MS.gene29448.t1 |
| chr5.3 | CDS | 64040111 | 64040212 | 64040111 | ID=cds.MS.gene29448.t1;Parent=MS.gene29448.t1 |
| chr5.3 | exon | 64041485 | 64041543 | 64041485 | ID=MS.gene29448.t1.exon8;Parent=MS.gene29448.t1 |
| chr5.3 | CDS | 64041485 | 64041543 | 64041485 | ID=cds.MS.gene29448.t1;Parent=MS.gene29448.t1 |
| chr5.3 | exon | 64041625 | 64041799 | 64041625 | ID=MS.gene29448.t1.exon9;Parent=MS.gene29448.t1 |
| chr5.3 | CDS | 64041625 | 64041799 | 64041625 | ID=cds.MS.gene29448.t1;Parent=MS.gene29448.t1 |
| chr5.3 | exon | 64042798 | 64042971 | 64042798 | ID=MS.gene29448.t1.exon10;Parent=MS.gene29448.t1 |
| chr5.3 | CDS | 64042798 | 64042971 | 64042798 | ID=cds.MS.gene29448.t1;Parent=MS.gene29448.t1 |
| chr5.3 | exon | 64044376 | 64044526 | 64044376 | ID=MS.gene29448.t1.exon11;Parent=MS.gene29448.t1 |
| chr5.3 | CDS | 64044376 | 64044526 | 64044376 | ID=cds.MS.gene29448.t1;Parent=MS.gene29448.t1 |
| chr5.3 | exon | 64044951 | 64045008 | 64044951 | ID=MS.gene29448.t1.exon12;Parent=MS.gene29448.t1 |
| chr5.3 | CDS | 64044951 | 64045008 | 64044951 | ID=cds.MS.gene29448.t1;Parent=MS.gene29448.t1 |
| chr5.3 | exon | 64045133 | 64045238 | 64045133 | ID=MS.gene29448.t1.exon13;Parent=MS.gene29448.t1 |
| chr5.3 | CDS | 64045133 | 64045238 | 64045133 | ID=cds.MS.gene29448.t1;Parent=MS.gene29448.t1 |
| chr5.3 | exon | 64045618 | 64045821 | 64045618 | ID=MS.gene29448.t1.exon14;Parent=MS.gene29448.t1 |
| chr5.3 | CDS | 64045618 | 64045821 | 64045618 | ID=cds.MS.gene29448.t1;Parent=MS.gene29448.t1 |
| Gene Sequence |
| Protein sequence |
