AlfalfaGEDB | MS.gene063518

M. sativa cultivar XinJiangDaYe / MS.gene063518

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02701.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063518.t1	XP_013458026.1	96.6	322	9	1	1	322	1	320	2.80E-101	378.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063518.t1	Q8LDR0	51.8	301	78	9	40	319	51	305	8.5e-30	132.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063518.t1	A0A072UQU9	96.6	322	9	1	1	322	1	320	2.0e-101	378.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene063518.t1	TF	C2C2-Dof

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049217	MS.gene063518	0.802988	4.32E-49	-1.69E-46
MS.gene049633	MS.gene063518	0.81192	5.50E-51	-1.69E-46
MS.gene050432	MS.gene063518	0.811672	6.23E-51	-1.69E-46
MS.gene05081	MS.gene063518	0.827696	1.36E-54	-1.69E-46
MS.gene05105	MS.gene063518	0.811604	6.44E-51	-1.69E-46
MS.gene051255	MS.gene063518	0.802869	4.57E-49	-1.69E-46
MS.gene051360	MS.gene063518	0.827354	1.64E-54	-1.69E-46
MS.gene051734	MS.gene063518	0.810825	9.51E-51	-1.69E-46
MS.gene051810	MS.gene063518	0.800193	1.62E-48	-1.69E-46
MS.gene051838	MS.gene063518	0.802301	5.99E-49	-1.69E-46
MS.gene051858	MS.gene063518	0.836122	1.13E-56	-1.69E-46
MS.gene052271	MS.gene063518	0.813875	2.05E-51	-1.69E-46
MS.gene052481	MS.gene063518	0.829303	5.55E-55	-1.69E-46
MS.gene052484	MS.gene063518	0.849206	3.75E-60	-1.69E-46
MS.gene052485	MS.gene063518	0.822324	2.52E-53	-1.69E-46
MS.gene052870	MS.gene063518	0.810031	1.41E-50	-1.69E-46
MS.gene052872	MS.gene063518	0.807512	4.87E-50	-1.69E-46
MS.gene052999	MS.gene063518	0.822372	2.45E-53	-1.69E-46
MS.gene053207	MS.gene063518	0.822326	2.52E-53	-1.69E-46
MS.gene053209	MS.gene063518	0.819093	1.39E-52	-1.69E-46
MS.gene053449	MS.gene063518	0.811504	6.77E-51	-1.69E-46
MS.gene053824	MS.gene063518	0.811478	6.86E-51	-1.69E-46
MS.gene054600	MS.gene063518	0.823341	1.46E-53	-1.69E-46
MS.gene05492	MS.gene063518	0.814105	1.82E-51	-1.69E-46
MS.gene05494	MS.gene063518	0.817301	3.54E-52	-1.69E-46
MS.gene055206	MS.gene063518	0.851986	6.21E-61	-1.69E-46
MS.gene055289	MS.gene063518	0.805982	1.03E-49	-1.69E-46
MS.gene055647	MS.gene063518	0.809772	1.60E-50	-1.69E-46
MS.gene055722	MS.gene063518	0.837914	3.92E-57	-1.69E-46
MS.gene055941	MS.gene063518	0.800932	1.14E-48	-1.69E-46
MS.gene057076	MS.gene063518	0.821559	3.79E-53	-1.69E-46
MS.gene057289	MS.gene063518	0.824292	8.74E-54	-1.69E-46
MS.gene057331	MS.gene063518	0.808085	3.68E-50	-1.69E-46
MS.gene057332	MS.gene063518	0.802087	6.63E-49	-1.69E-46
MS.gene057749	MS.gene063518	0.809939	1.48E-50	-1.69E-46
MS.gene057823	MS.gene063518	0.810078	1.38E-50	-1.69E-46
MS.gene058338	MS.gene063518	0.81407	1.86E-51	-1.69E-46
MS.gene058444	MS.gene063518	0.806557	7.76E-50	-1.69E-46
MS.gene058588	MS.gene063518	0.803083	4.13E-49	-1.69E-46
MS.gene059023	MS.gene063518	0.801538	8.59E-49	-1.69E-46
MS.gene059129	MS.gene063518	0.825196	5.35E-54	-1.69E-46
MS.gene059695	MS.gene063518	0.800009	1.76E-48	-1.69E-46
MS.gene059902	MS.gene063518	0.830356	3.08E-55	-1.69E-46
MS.gene060469	MS.gene063518	0.838358	3.02E-57	-1.69E-46
MS.gene060837	MS.gene063518	0.839352	1.67E-57	-1.69E-46
MS.gene061231	MS.gene063518	0.803711	3.06E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063518.t1	MTR_4g109980	97.516	322	6	1	1	322	1	320	0.0	644
MS.gene063518.t1	MTR_2g059540	63.000	100	37	0	40	139	31	130	4.22e-34	124
MS.gene063518.t1	MTR_2g096740	63.043	92	25	1	40	131	62	144	6.77e-34	126
MS.gene063518.t1	MTR_4g088580	58.879	107	42	2	18	123	32	137	8.36e-34	128
MS.gene063518.t1	MTR_2g096740	63.043	92	25	1	40	131	77	159	8.83e-34	126
MS.gene063518.t1	MTR_8g479350	82.759	58	10	0	36	93	64	121	1.35e-31	121
MS.gene063518.t1	MTR_8g015840	80.000	65	12	1	37	100	17	81	1.76e-31	117
MS.gene063518.t1	MTR_4g063780	67.606	71	23	0	37	107	72	142	3.15e-31	120
MS.gene063518.t1	MTR_4g022370	40.361	166	78	3	19	170	54	212	1.22e-30	119
MS.gene063518.t1	MTR_7g059400	47.581	124	47	2	27	150	63	168	1.69e-30	118
MS.gene063518.t1	MTR_8g068210	70.833	72	18	1	39	107	31	102	7.47e-30	116
MS.gene063518.t1	MTR_8g079060	60.759	79	31	0	37	115	26	104	7.51e-30	114
MS.gene063518.t1	MTR_4g461080	70.270	74	16	1	18	91	13	80	9.12e-30	114
MS.gene063518.t1	MTR_7g024670	82.456	57	10	0	41	97	78	134	1.34e-29	116
MS.gene063518.t1	MTR_5g031440	83.333	54	9	0	37	90	40	93	1.71e-29	115
MS.gene063518.t1	MTR_3g091820	64.865	74	20	1	23	96	32	99	2.31e-29	114
MS.gene063518.t1	MTR_3g077750	56.000	100	38	1	23	122	50	143	6.46e-29	114
MS.gene063518.t1	MTR_2g093220	66.667	81	24	1	37	114	37	117	7.50e-29	112
MS.gene063518.t1	MTR_2g014060	86.275	51	7	0	41	91	26	76	2.37e-28	112
MS.gene063518.t1	MTR_8g027295	53.191	94	36	1	21	114	13	98	3.78e-28	110
MS.gene063518.t1	MTR_3g090430	74.194	62	16	0	42	103	53	114	6.66e-28	111
MS.gene063518.t1	MTR_1g056810	77.966	59	13	0	36	94	8	66	4.26e-27	108
MS.gene063518.t1	MTR_1g077600	72.414	58	16	0	37	94	40	97	6.06e-27	107
MS.gene063518.t1	MTR_1g077600	72.414	58	16	0	37	94	40	97	7.09e-27	107
MS.gene063518.t1	MTR_7g010950	71.186	59	17	0	38	96	133	191	1.74e-26	109
MS.gene063518.t1	MTR_2g013370	70.769	65	18	1	30	94	36	99	2.74e-26	105
MS.gene063518.t1	MTR_8g044220	68.966	58	18	0	38	95	116	173	3.23e-26	108
MS.gene063518.t1	MTR_2g014170	84.000	50	8	0	39	88	44	93	5.30e-26	105
MS.gene063518.t1	MTR_2g016030	68.421	57	18	0	37	93	44	100	9.75e-26	100
MS.gene063518.t1	MTR_7g086780	46.316	95	51	0	38	132	97	191	1.67e-25	105
MS.gene063518.t1	MTR_6g012450	67.797	59	19	0	38	96	134	192	2.24e-25	106
MS.gene063518.t1	MTR_4g082060	67.857	56	18	0	38	93	120	175	1.50e-24	103
MS.gene063518.t1	MTR_3g435480	58.904	73	23	1	31	96	120	192	3.15e-24	102
MS.gene063518.t1	MTR_5g041420	67.797	59	19	0	38	96	136	194	5.41e-24	100
MS.gene063518.t1	MTR_6g027460	46.000	100	50	1	38	137	94	189	6.07e-24	100
MS.gene063518.t1	MTR_6g027450	63.158	57	21	0	38	94	87	143	3.20e-23	98.2
MS.gene063518.t1	MTR_5g041530	63.077	65	23	1	30	93	129	193	3.72e-23	99.0
MS.gene063518.t1	MTR_5g041380	66.102	59	20	0	38	96	125	183	6.99e-23	97.8
MS.gene063518.t1	MTR_5g041400	66.102	59	20	0	38	96	117	175	1.35e-22	97.1
MS.gene063518.t1	MTR_4g089095	35.714	168	79	3	38	203	48	188	3.82e-16	77.8
MS.gene063518.t1	MTR_4g089095	35.714	168	79	3	38	203	14	154	4.76e-16	77.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063518.t1	AT5G60850	42.857	322	114	12	23	322	34	307	4.01e-55	182
MS.gene063518.t1	AT1G51700	76.712	73	16	1	37	108	30	102	1.75e-33	122
MS.gene063518.t1	AT5G65590	75.758	66	16	0	36	101	37	102	1.65e-32	123
MS.gene063518.t1	AT5G60200	67.949	78	19	1	39	116	54	125	2.88e-32	121
MS.gene063518.t1	AT5G66940	56.989	93	34	1	36	122	28	120	2.09e-31	117
MS.gene063518.t1	AT3G45610	66.234	77	19	1	37	113	37	106	4.37e-31	117
MS.gene063518.t1	AT2G37590	68.116	69	22	0	27	95	76	144	9.81e-31	119
MS.gene063518.t1	AT2G28510	42.778	180	79	5	35	202	43	210	2.73e-30	116
MS.gene063518.t1	AT1G07640	79.310	58	12	0	40	97	21	78	3.01e-30	116
MS.gene063518.t1	AT1G07640	79.310	58	12	0	40	97	77	134	4.09e-30	117
MS.gene063518.t1	AT1G07640	79.310	58	12	0	40	97	85	142	5.45e-30	117
MS.gene063518.t1	AT2G28810	83.019	53	9	0	39	91	76	128	1.14e-29	115
MS.gene063518.t1	AT3G61850	65.000	80	20	1	24	103	54	125	1.17e-29	114
MS.gene063518.t1	AT3G61850	65.000	80	20	1	24	103	54	125	1.17e-29	114
MS.gene063518.t1	AT4G38000	63.750	80	20	2	20	90	12	91	1.24e-29	114
MS.gene063518.t1	AT3G52440	71.053	76	17	2	27	102	14	84	1.25e-29	114
MS.gene063518.t1	AT3G61850	65.000	80	20	1	24	103	66	137	1.38e-29	115
MS.gene063518.t1	AT2G28810	83.019	53	9	0	39	91	93	145	1.64e-29	115
MS.gene063518.t1	AT3G52440	71.053	76	17	2	27	102	33	103	1.68e-29	114
MS.gene063518.t1	AT3G61850	65.000	80	20	1	24	103	54	125	1.89e-29	115
MS.gene063518.t1	AT3G61850	65.000	80	20	1	24	103	66	137	2.00e-29	115
MS.gene063518.t1	AT3G50410	61.905	84	28	1	18	97	4	87	2.65e-29	113
MS.gene063518.t1	AT3G21270	88.679	53	6	0	40	92	29	81	2.72e-29	111
MS.gene063518.t1	AT3G55370	69.118	68	21	0	35	102	71	138	3.88e-29	114
MS.gene063518.t1	AT3G55370	69.118	68	21	0	35	102	71	138	5.48e-29	114
MS.gene063518.t1	AT3G55370	69.118	68	21	0	35	102	116	183	7.63e-29	114
MS.gene063518.t1	AT1G28310	77.966	59	13	0	40	98	27	85	7.91e-29	113
MS.gene063518.t1	AT5G02460	75.000	60	15	0	35	94	90	149	8.83e-29	114
MS.gene063518.t1	AT4G21050	55.882	102	38	2	42	137	26	126	1.16e-28	110
MS.gene063518.t1	AT1G28310	77.966	59	13	0	40	98	51	109	1.37e-28	113
MS.gene063518.t1	AT4G24060	63.514	74	21	1	21	94	40	107	3.26e-28	112
MS.gene063518.t1	AT5G62940	69.565	69	15	1	22	90	61	123	7.22e-28	111
MS.gene063518.t1	AT1G21340	80.357	56	11	0	42	97	40	95	7.48e-28	109
MS.gene063518.t1	AT4G00940	64.103	78	28	0	37	114	65	142	2.88e-26	105
MS.gene063518.t1	AT4G00940	64.103	78	28	0	37	114	65	142	2.88e-26	105
MS.gene063518.t1	AT4G00940	64.103	78	28	0	37	114	65	142	2.88e-26	105
MS.gene063518.t1	AT1G29160	71.429	56	16	0	38	93	60	115	1.65e-25	100
MS.gene063518.t1	AT2G34140	71.429	56	16	0	38	93	56	111	1.92e-25	100
MS.gene063518.t1	AT3G47500	69.492	59	18	0	38	96	108	166	2.15e-25	105
MS.gene063518.t1	AT2G46590	54.321	81	29	1	7	87	43	115	3.17e-25	104
MS.gene063518.t1	AT2G46590	54.321	81	29	1	7	87	55	127	3.50e-25	104
MS.gene063518.t1	AT1G64620	61.194	67	20	1	23	89	38	98	3.69e-25	103
MS.gene063518.t1	AT5G39660	69.643	56	17	0	38	93	136	191	9.60e-25	104
MS.gene063518.t1	AT5G39660	69.643	56	17	0	38	93	136	191	9.60e-25	104
MS.gene063518.t1	AT5G39660	69.643	56	17	0	38	93	136	191	9.60e-25	104
MS.gene063518.t1	AT5G62430	64.407	59	21	0	38	96	52	110	3.18e-24	100
MS.gene063518.t1	AT1G69570	69.643	56	17	0	38	93	130	185	5.00e-24	101
MS.gene063518.t1	AT1G47655	74.510	51	13	0	38	88	27	77	5.10e-24	97.8
MS.gene063518.t1	AT1G26790	66.071	56	19	0	38	93	109	164	3.46e-23	99.0
MS.gene063518.t1	AT4G21040	42.069	145	67	3	42	169	27	171	4.60e-23	95.9
MS.gene063518.t1	AT4G21080	68.966	58	17	1	42	98	27	84	1.34e-21	92.4
MS.gene063518.t1	AT4G21030	55.882	68	27	1	42	109	23	87	4.93e-16	75.9

Find 94 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCTAGAGCGTTTGGATTTC+CGG	0.179446	8.4:-17786555	None:intergenic
TTCTGGTTCTGGATCGTTGC+TGG	0.241672	8.4:+17787044	MS.gene063518:CDS
GTTATTGTAGTAACAGAATT+TGG	0.266870	8.4:-17786437	None:intergenic
TCCCCGTCGGTGGCGGTTGC+CGG	0.275837	8.4:+17786536	MS.gene063518:CDS
GAGGACATTTCATGACTTCT+TGG	0.303639	8.4:-17786396	None:intergenic
AAAACAAGCAACGGTGGATT+TGG	0.307667	8.4:+17787099	MS.gene063518:CDS
GGTTCAGGTTGTTGTTGTTT+TGG	0.310471	8.4:-17786375	None:intergenic
GAGACATTGCCGTTTGGTTT+TGG	0.311028	8.4:+17786844	MS.gene063518:CDS
CGGTAACGCTCATCAGCTTT+TGG	0.328939	8.4:-17786942	None:intergenic
ACGGTAATGTTAACGTTAAC+GGG	0.351619	8.4:+17786887	MS.gene063518:CDS
CAAAAGCTGATGAGCGTTAC+CGG	0.354847	8.4:+17786943	MS.gene063518:CDS
ACACCGTTGATCATGGTTAC+TGG	0.364180	8.4:+17787181	MS.gene063518:CDS
GACTTATTCTCCGGCGCCTC+CGG	0.380062	8.4:-17786628	None:intergenic
GTAACAGAATTTGGTGTTGA+TGG	0.387408	8.4:-17786428	None:intergenic
TTCTTCTCCGGCGGTGGTGT+TGG	0.390782	8.4:-17786718	None:intergenic
ATTTCATGACTTCTTGGTTC+AGG	0.391447	8.4:-17786390	None:intergenic
TAACCATGATCAACGGTGTT+AGG	0.392627	8.4:-17787177	None:intergenic
AACGGTAATGTTAACGTTAA+CGG	0.394532	8.4:+17786886	MS.gene063518:CDS
ATAGGTGCTGGTGCTGGTGG+GGG	0.397991	8.4:+17787015	MS.gene063518:CDS
AATATAGGTGCTGGTGCTGG+TGG	0.411942	8.4:+17787012	MS.gene063518:CDS
GGCGCCTCCGGTGGAGTTGT+CGG	0.413936	8.4:-17786616	None:intergenic
TATAGGTGCTGGTGCTGGTG+GGG	0.419125	8.4:+17787014	MS.gene063518:CDS
CAACGGTGGATTTGGATCGT+TGG	0.423030	8.4:+17787107	MS.gene063518:CDS
ATATAGGTGCTGGTGCTGGT+GGG	0.429106	8.4:+17787013	MS.gene063518:CDS
CACCGCAGCGGCGGTGAGGC+TGG	0.433116	8.4:-17786677	None:intergenic
CGCCACCGACGGGGACGTTA+CGG	0.435705	8.4:-17786528	None:intergenic
AAACGGCAATGTCTCGTTAG+AGG	0.437377	8.4:-17786836	None:intergenic
GGTTCCTCCGGCGCCTCCGA+TGG	0.440934	8.4:-17786308	None:intergenic
AGACGCATTGAAGTTGTTAC+CGG	0.443650	8.4:+17786987	MS.gene063518:CDS
GTGGATTTGGATCGTTGGAT+TGG	0.444872	8.4:+17787112	MS.gene063518:CDS
CTTCCTAACACCGTTGATCA+TGG	0.449712	8.4:+17787174	MS.gene063518:CDS
TTCCGGCAACCGCCACCGAC+GGG	0.461760	8.4:-17786538	None:intergenic
CAGCCTCACCGCCGCTGCGG+TGG	0.464633	8.4:+17786678	MS.gene063518:CDS
GCCTCCACCACTGAAGAATC+TGG	0.466306	8.4:-17786329	None:intergenic
TCACACTCAATGGTCTGATC+AGG	0.473669	8.4:+17787206	MS.gene063518:CDS
AAGTTGTTACCGGAGAATAT+AGG	0.476958	8.4:+17786997	MS.gene063518:CDS
TAACGTTAACATTACCGTTA+CGG	0.477277	8.4:-17786882	None:intergenic
GATTCACTTGAACAAGGAAC+AGG	0.481996	8.4:+17786763	MS.gene063518:CDS
CGTAACGTCCCCGTCGGTGG+CGG	0.482120	8.4:+17786529	MS.gene063518:CDS
TCAGTGGTGGAGGCGGTGAT+CGG	0.482140	8.4:+17786338	MS.gene063518:CDS
GGAACTCTCACTGCTGGAAT+GGG	0.482309	8.4:-17786656	None:intergenic
TGGAACTCTCACTGCTGGAA+TGG	0.493236	8.4:-17786657	None:intergenic
TCTAACGAGACATTGCCGTT+TGG	0.499608	8.4:+17786838	MS.gene063518:CDS
GACAACTCCACCGGAGGCGC+CGG	0.504458	8.4:+17786618	MS.gene063518:CDS
CAACATTCCATCGGAGGCGC+CGG	0.516567	8.4:+17786301	MS.gene063518:CDS
GAGGCTGGAACTCTCACTGC+TGG	0.519133	8.4:-17786662	None:intergenic
TTTCCGGCAACCGCCACCGA+CGG	0.522246	8.4:-17786539	None:intergenic
TGCCGACGTTACTGGACTAA+AGG	0.527907	8.4:+17786496	MS.gene063518:CDS
CTCCGTAACGTCCCCGTCGG+TGG	0.530524	8.4:+17786526	MS.gene063518:CDS
TTATTCTCCGGCGCCTCCGG+TGG	0.532938	8.4:-17786625	None:intergenic
CAGCCACCGCAGCGGCGGTG+AGG	0.533594	8.4:-17786681	None:intergenic
ACCAGATTCTTCAGTGGTGG+AGG	0.541259	8.4:+17786328	MS.gene063518:CDS
ATGATGCGTCGTCGTTTCAG+TGG	0.549551	8.4:+17786914	MS.gene063518:CDS
CATTCCATCGGAGGCGCCGG+AGG	0.561089	8.4:+17786304	MS.gene063518:CDS
TTTGGATCGTTGGATTGGAA+TGG	0.568406	8.4:+17787117	MS.gene063518:CDS
TGGGAGTTAGACTTATTCTC+CGG	0.569296	8.4:-17786637	None:intergenic
ACTGAAGAATCTGGTTCCTC+CGG	0.571115	8.4:-17786320	None:intergenic
AGATTCTTCAGTGGTGGAGG+CGG	0.571847	8.4:+17786331	MS.gene063518:CDS
TTCAGAACCAACACCACCGC+CGG	0.573448	8.4:+17786711	MS.gene063518:CDS
GTTACTGGAATCACACTCAA+TGG	0.574806	8.4:+17787196	MS.gene063518:CDS
TTACCGGAGAATATAGGTGC+TGG	0.577924	8.4:+17787003	MS.gene063518:CDS
CGGTAAAACAGTGGAATCTG+AGG	0.581795	8.4:-17786596	None:intergenic
GTCCTCCGTAACGTCCCCGT+CGG	0.588295	8.4:+17786523	MS.gene063518:CDS
TGTTTCAGCCACCGCAGCGG+CGG	0.589914	8.4:-17786686	None:intergenic
GAGAATATAGGTGCTGGTGC+TGG	0.591108	8.4:+17787009	MS.gene063518:CDS
TTCCAGCCTCACCGCCGCTG+CGG	0.594936	8.4:+17786675	MS.gene063518:CDS
GCACCAGCACCTATATTCTC+CGG	0.599864	8.4:-17787006	None:intergenic
AGAAGTGACGAGGCTGAGAG+AGG	0.604907	8.4:-17786465	None:intergenic
GAACAAGGAACAGGTGATTG+TGG	0.605175	8.4:+17786772	MS.gene063518:CDS
TCCGGCAACCGCCACCGACG+GGG	0.606442	8.4:-17786537	None:intergenic
GGAACCAGATTCTTCAGTGG+TGG	0.610190	8.4:+17786325	MS.gene063518:CDS
TTACCGATACCAAAACCAAA+CGG	0.611130	8.4:-17786853	None:intergenic
CTGCAGAGTAAAACAAGCAA+CGG	0.614794	8.4:+17787090	MS.gene063518:CDS
TCGTTGGATTGGAATGGTGA+CGG	0.617509	8.4:+17787123	MS.gene063518:CDS
GAGAATGATTCACTTGAACA+AGG	0.621864	8.4:+17786757	MS.gene063518:CDS
GACGGTGGTGCAGATCATCA+AGG	0.629904	8.4:+17787141	MS.gene063518:CDS
TCTGGATCGTTGCTGGACCA+TGG	0.630293	8.4:+17787051	MS.gene063518:CDS
CAGAGTAAAACAAGCAACGG+TGG	0.633363	8.4:+17787093	MS.gene063518:CDS
GAGATACAACATTCCATCGG+AGG	0.634291	8.4:+17786295	MS.gene063518:CDS
GCAAAGAGAAATCAATACCA+TGG	0.638840	8.4:-17787068	None:intergenic
AACTGTTTCAGCCACCGCAG+CGG	0.652527	8.4:-17786689	None:intergenic
ATCGGTAACGATAACCGTAA+CGG	0.654058	8.4:+17786868	MS.gene063518:CDS
TAAAACAGTGGAATCTGAGG+AGG	0.660488	8.4:-17786593	None:intergenic
TTTACCGACAACTCCACCGG+AGG	0.660654	8.4:+17786612	MS.gene063518:CDS
CGCCTTTAGTCCAGTAACGT+CGG	0.661444	8.4:-17786498	None:intergenic
CACCGACGGGGACGTTACGG+AGG	0.667570	8.4:-17786525	None:intergenic
CAGGAGATACAACATTCCAT+CGG	0.667929	8.4:+17786292	MS.gene063518:CDS
TGGAGTTGTCGGTAAAACAG+TGG	0.668985	8.4:-17786605	None:intergenic
ATTCCAGTAACCATGATCAA+CGG	0.674918	8.4:-17787184	None:intergenic
CCGGAGAAGAAAATCAACAA+AGG	0.680379	8.4:+17786730	MS.gene063518:CDS
CGACGTTACTGGACTAAAGG+CGG	0.694270	8.4:+17786499	MS.gene063518:CDS
GGAGGAACCAGATTCTTCAG+TGG	0.695984	8.4:+17786322	MS.gene063518:CDS
GTGTTGATGGAGTCACAGCG+AGG	0.726737	8.4:-17786415	None:intergenic
TTGGATTGGAATGGTGACGG+TGG	0.733534	8.4:+17787126	MS.gene063518:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GTTATTGTAGTAACAGAATT+TGG	-	chr8.4:17786440-17786459	None:intergenic	25.0%
	AACGGTAATGTTAACGTTAA+CGG	+	chr8.4:17786886-17786905	MS.gene063518:CDS	30.0%
	TAACGTTAACATTACCGTTA+CGG	-	chr8.4:17786885-17786904	None:intergenic	30.0%
	AAGTTGTTACCGGAGAATAT+AGG	+	chr8.4:17786997-17787016	MS.gene063518:CDS	35.0%
	ACGGTAATGTTAACGTTAAC+GGG	+	chr8.4:17786887-17786906	MS.gene063518:CDS	35.0%
	ATTCCAGTAACCATGATCAA+CGG	-	chr8.4:17787187-17787206	None:intergenic	35.0%
	ATTTCATGACTTCTTGGTTC+AGG	-	chr8.4:17786393-17786412	None:intergenic	35.0%
	GAGAATGATTCACTTGAACA+AGG	+	chr8.4:17786757-17786776	MS.gene063518:CDS	35.0%
	GCAAAGAGAAATCAATACCA+TGG	-	chr8.4:17787071-17787090	None:intergenic	35.0%
	TTACCGATACCAAAACCAAA+CGG	-	chr8.4:17786856-17786875	None:intergenic	35.0%
!!	GTAACAGAATTTGGTGTTGA+TGG	-	chr8.4:17786431-17786450	None:intergenic	35.0%
!!	TTCATGATGTTTTTGATCGC+CGG	-	chr8.4:17786965-17786984	None:intergenic	35.0%
!!!	AGGTTGTTGTTGTTTTGGTT+GGG	-	chr8.4:17786373-17786392	None:intergenic	35.0%
!!!	CCTTTGTTGATTTTCTTCTC+CGG	-	chr8.4:17786733-17786752	None:intergenic	35.0%
!!!	TCTGAGGAGGAAGATTTTTT+TGG	-	chr8.4:17786583-17786602	None:intergenic	35.0%
	AGACGCATTGAAGTTGTTAC+CGG	+	chr8.4:17786987-17787006	MS.gene063518:CDS	40.0%
	ATCGGTAACGATAACCGTAA+CGG	+	chr8.4:17786868-17786887	MS.gene063518:CDS	40.0%
	CAGGAGATACAACATTCCAT+CGG	+	chr8.4:17786292-17786311	MS.gene063518:CDS	40.0%
	CCGGAGAAGAAAATCAACAA+AGG	+	chr8.4:17786730-17786749	MS.gene063518:CDS	40.0%
	CTGCAGAGTAAAACAAGCAA+CGG	+	chr8.4:17787090-17787109	MS.gene063518:CDS	40.0%
	GAGGACATTTCATGACTTCT+TGG	-	chr8.4:17786399-17786418	None:intergenic	40.0%
	GATTCACTTGAACAAGGAAC+AGG	+	chr8.4:17786763-17786782	MS.gene063518:CDS	40.0%
	GTTACTGGAATCACACTCAA+TGG	+	chr8.4:17787196-17787215	MS.gene063518:CDS	40.0%
	TAAAACAGTGGAATCTGAGG+AGG	-	chr8.4:17786596-17786615	None:intergenic	40.0%
	TAACCATGATCAACGGTGTT+AGG	-	chr8.4:17787180-17787199	None:intergenic	40.0%
	TGGGAGTTAGACTTATTCTC+CGG	-	chr8.4:17786640-17786659	None:intergenic	40.0%
!	AAAACAAGCAACGGTGGATT+TGG	+	chr8.4:17787099-17787118	MS.gene063518:CDS	40.0%
!	TTTGGATCGTTGGATTGGAA+TGG	+	chr8.4:17787117-17787136	MS.gene063518:CDS	40.0%
!!!	CAGGTTGTTGTTGTTTTGGT+TGG	-	chr8.4:17786374-17786393	None:intergenic	40.0%
!!!	GGTTCAGGTTGTTGTTGTTT+TGG	-	chr8.4:17786378-17786397	None:intergenic	40.0%
!!!	TTGCCGTTTGGTTTTGGTAT+CGG	+	chr8.4:17786850-17786869	MS.gene063518:CDS	40.0%
!!!	TTGTTGATTTTCTTCTCCGG+CGG	-	chr8.4:17786730-17786749	None:intergenic	40.0%
!!!	TTTTGGTTTGCTAGAGCGTT+TGG	-	chr8.4:17786566-17786585	None:intergenic	40.0%
	AAACGGCAATGTCTCGTTAG+AGG	-	chr8.4:17786839-17786858	None:intergenic	45.0%
	ACACCGTTGATCATGGTTAC+TGG	+	chr8.4:17787181-17787200	MS.gene063518:CDS	45.0%
	CAAAAGCTGATGAGCGTTAC+CGG	+	chr8.4:17786943-17786962	MS.gene063518:CDS	45.0%
	CAGAGTAAAACAAGCAACGG+TGG	+	chr8.4:17787093-17787112	MS.gene063518:CDS	45.0%
	CGGTAAAACAGTGGAATCTG+AGG	-	chr8.4:17786599-17786618	None:intergenic	45.0%
	CTTCCTAACACCGTTGATCA+TGG	+	chr8.4:17787174-17787193	MS.gene063518:CDS	45.0%
	GAACAAGGAACAGGTGATTG+TGG	+	chr8.4:17786772-17786791	MS.gene063518:CDS	45.0%
	GAGATACAACATTCCATCGG+AGG	+	chr8.4:17786295-17786314	MS.gene063518:CDS	45.0%
	GTGGATTTGGATCGTTGGAT+TGG	+	chr8.4:17787112-17787131	MS.gene063518:CDS	45.0%
	TCACACTCAATGGTCTGATC+AGG	+	chr8.4:17787206-17787225	MS.gene063518:CDS	45.0%
	TCTAACGAGACATTGCCGTT+TGG	+	chr8.4:17786838-17786857	MS.gene063518:CDS	45.0%
	TGGAGTTGTCGGTAAAACAG+TGG	-	chr8.4:17786608-17786627	None:intergenic	45.0%
	TTACCGGAGAATATAGGTGC+TGG	+	chr8.4:17787003-17787022	MS.gene063518:CDS	45.0%
!	ACTGAAGAATCTGGTTCCTC+CGG	-	chr8.4:17786323-17786342	None:intergenic	45.0%
!	CAGTTTTTGCAGAAGTGACG+AGG	-	chr8.4:17786478-17786497	None:intergenic	45.0%
!	TCGTTGGATTGGAATGGTGA+CGG	+	chr8.4:17787123-17787142	MS.gene063518:CDS	45.0%
!	TGCTAGAGCGTTTGGATTTC+CGG	-	chr8.4:17786558-17786577	None:intergenic	45.0%
!	TGTTTTACCGACAACTCCAC+CGG	+	chr8.4:17786609-17786628	MS.gene063518:CDS	45.0%
!!	GAGACATTGCCGTTTGGTTT+TGG	+	chr8.4:17786844-17786863	MS.gene063518:CDS	45.0%
	ACCAGATTCTTCAGTGGTGG+AGG	+	chr8.4:17786328-17786347	MS.gene063518:CDS	50.0%
	ATGATGCGTCGTCGTTTCAG+TGG	+	chr8.4:17786914-17786933	MS.gene063518:CDS	50.0%
	CGACGTTACTGGACTAAAGG+CGG	+	chr8.4:17786499-17786518	MS.gene063518:CDS	50.0%
	CGCCTTTAGTCCAGTAACGT+CGG	-	chr8.4:17786501-17786520	None:intergenic	50.0%
	GCAAAAACTGCCGACGTTAC+TGG	+	chr8.4:17786488-17786507	MS.gene063518:CDS	50.0%
	GCACCAGCACCTATATTCTC+CGG	-	chr8.4:17787009-17787028	None:intergenic	50.0%
	GGAACCAGATTCTTCAGTGG+TGG	+	chr8.4:17786325-17786344	MS.gene063518:CDS	50.0%
	GGAACTCTCACTGCTGGAAT+GGG	-	chr8.4:17786659-17786678	None:intergenic	50.0%
	GGAGGAACCAGATTCTTCAG+TGG	+	chr8.4:17786322-17786341	MS.gene063518:CDS	50.0%
	TGCCGACGTTACTGGACTAA+AGG	+	chr8.4:17786496-17786515	MS.gene063518:CDS	50.0%
	TGGAACTCTCACTGCTGGAA+TGG	-	chr8.4:17786660-17786679	None:intergenic	50.0%
!	AGATTCTTCAGTGGTGGAGG+CGG	+	chr8.4:17786331-17786350	MS.gene063518:CDS	50.0%
!	CAACGGTGGATTTGGATCGT+TGG	+	chr8.4:17787107-17787126	MS.gene063518:CDS	50.0%
!	CGGTAACGCTCATCAGCTTT+TGG	-	chr8.4:17786945-17786964	None:intergenic	50.0%
!	TTGATTTTCTTCTCCGGCGG+TGG	-	chr8.4:17786727-17786746	None:intergenic	50.0%
!!	AATATAGGTGCTGGTGCTGG+TGG	+	chr8.4:17787012-17787031	MS.gene063518:CDS	50.0%
!!	ATATAGGTGCTGGTGCTGGT+GGG	+	chr8.4:17787013-17787032	MS.gene063518:CDS	50.0%
!!	GAGAATATAGGTGCTGGTGC+TGG	+	chr8.4:17787009-17787028	MS.gene063518:CDS	50.0%
!!	TTCTGGTTCTGGATCGTTGC+TGG	+	chr8.4:17787044-17787063	MS.gene063518:CDS	50.0%
!!	TTGGATTGGAATGGTGACGG+TGG	+	chr8.4:17787126-17787145	MS.gene063518:CDS	50.0%
	AACTGTTTCAGCCACCGCAG+CGG	-	chr8.4:17786692-17786711	None:intergenic	55.0%
	AGAAGTGACGAGGCTGAGAG+AGG	-	chr8.4:17786468-17786487	None:intergenic	55.0%
	GACGGTGGTGCAGATCATCA+AGG	+	chr8.4:17787141-17787160	MS.gene063518:CDS	55.0%
	GCCTCCACCACTGAAGAATC+TGG	-	chr8.4:17786332-17786351	None:intergenic	55.0%
	TCTGGATCGTTGCTGGACCA+TGG	+	chr8.4:17787051-17787070	MS.gene063518:CDS	55.0%
	TTCAGAACCAACACCACCGC+CGG	+	chr8.4:17786711-17786730	MS.gene063518:CDS	55.0%
	TTTACCGACAACTCCACCGG+AGG	+	chr8.4:17786612-17786631	MS.gene063518:CDS	55.0%
!	GTGTTGATGGAGTCACAGCG+AGG	-	chr8.4:17786418-17786437	None:intergenic	55.0%
!!	TATAGGTGCTGGTGCTGGTG+GGG	+	chr8.4:17787014-17787033	MS.gene063518:CDS	55.0%
	GACTTATTCTCCGGCGCCTC+CGG	-	chr8.4:17786631-17786650	None:intergenic	60.0%
	GAGGCTGGAACTCTCACTGC+TGG	-	chr8.4:17786665-17786684	None:intergenic	60.0%
	TTCTTCTCCGGCGGTGGTGT+TGG	-	chr8.4:17786721-17786740	None:intergenic	60.0%
!	CAACATTCCATCGGAGGCGC+CGG	+	chr8.4:17786301-17786320	MS.gene063518:CDS	60.0%
!	TCAGTGGTGGAGGCGGTGAT+CGG	+	chr8.4:17786338-17786357	MS.gene063518:CDS	60.0%
!!	ATAGGTGCTGGTGCTGGTGG+GGG	+	chr8.4:17787015-17787034	MS.gene063518:CDS	60.0%
!!	GGGGGTGGTTCTTCTGGTTC+TGG	+	chr8.4:17787033-17787052	MS.gene063518:CDS	60.0%
	GCTGGTGGGGGTGGTTCTTC+TGG	+	chr8.4:17787027-17787046	MS.gene063518:CDS	65.0%
	GTCCTCCGTAACGTCCCCGT+CGG	+	chr8.4:17786523-17786542	MS.gene063518:CDS	65.0%
	TGTTTCAGCCACCGCAGCGG+CGG	-	chr8.4:17786689-17786708	None:intergenic	65.0%
	TTATTCTCCGGCGCCTCCGG+TGG	-	chr8.4:17786628-17786647	None:intergenic	65.0%
	TTTCCGGCAACCGCCACCGA+CGG	-	chr8.4:17786542-17786561	None:intergenic	65.0%
	CACCGACGGGGACGTTACGG+AGG	-	chr8.4:17786528-17786547	None:intergenic	70.0%
	CGCCACCGACGGGGACGTTA+CGG	-	chr8.4:17786531-17786550	None:intergenic	70.0%
	CGTAACGTCCCCGTCGGTGG+CGG	+	chr8.4:17786529-17786548	MS.gene063518:CDS	70.0%
	CTCCGTAACGTCCCCGTCGG+TGG	+	chr8.4:17786526-17786545	MS.gene063518:CDS	70.0%
	GGCGCCTCCGGTGGAGTTGT+CGG	-	chr8.4:17786619-17786638	None:intergenic	70.0%
	TTCCAGCCTCACCGCCGCTG+CGG	+	chr8.4:17786675-17786694	MS.gene063518:CDS	70.0%
	TTCCGGCAACCGCCACCGAC+GGG	-	chr8.4:17786541-17786560	None:intergenic	70.0%
!	GACAACTCCACCGGAGGCGC+CGG	+	chr8.4:17786618-17786637	MS.gene063518:CDS	70.0%
!!	CATTCCATCGGAGGCGCCGG+AGG	+	chr8.4:17786304-17786323	MS.gene063518:CDS	70.0%
	GGTTCCTCCGGCGCCTCCGA+TGG	-	chr8.4:17786311-17786330	None:intergenic	75.0%
	TCCGGCAACCGCCACCGACG+GGG	-	chr8.4:17786540-17786559	None:intergenic	75.0%
!	TCCCCGTCGGTGGCGGTTGC+CGG	+	chr8.4:17786536-17786555	MS.gene063518:CDS	75.0%
!!	GGTGCTGGTGCTGGTGGGGG+TGG	+	chr8.4:17787018-17787037	MS.gene063518:CDS	75.0%
!	CACCGCAGCGGCGGTGAGGC+TGG	-	chr8.4:17786680-17786699	None:intergenic	80.0%
!	CAGCCACCGCAGCGGCGGTG+AGG	-	chr8.4:17786684-17786703	None:intergenic	80.0%
!	CAGCCTCACCGCCGCTGCGG+TGG	+	chr8.4:17786678-17786697	MS.gene063518:CDS	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	17786289	17787257	17786289	ID=MS.gene063518
chr8.4	mRNA	17786289	17787257	17786289	ID=MS.gene063518.t1;Parent=MS.gene063518
chr8.4	exon	17786289	17787257	17786289	ID=MS.gene063518.t1.exon1;Parent=MS.gene063518.t1
chr8.4	CDS	17786289	17787257	17786289	ID=cds.MS.gene063518.t1;Parent=MS.gene063518.t1

Gene Sequence

>MS.gene063518

ATGCAGGAGATACAACATTCCATCGGAGGCGCCGGAGGAACCAGATTCTTCAGTGGTGGAGGCGGTGATCGGCGACTCAGACCCAACCAAAACAACAACAACCTGAACCAAGAAGTCATGAAATGTCCTCGCTGTGACTCCATCAACACCAAATTCTGTTACTACAATAACTACAACCTCTCTCAGCCTCGTCACTTCTGCAAAAACTGCCGACGTTACTGGACTAAAGGCGGTGTCCTCCGTAACGTCCCCGTCGGTGGCGGTTGCCGGAAATCCAAACGCTCTAGCAAACCAAAAAAATCTTCCTCCTCAGATTCCACTGTTTTACCGACAACTCCACCGGAGGCGCCGGAGAATAAGTCTAACTCCCATTCCAGCAGTGAGAGTTCCAGCCTCACCGCCGCTGCGGTGGCTGAAACAGTTTCAGAACCAACACCACCGCCGGAGAAGAAAATCAACAAAGGTTTTGAGAATGATTCACTTGAACAAGGAACAGGTGATTGTGGTATATTCTCAGAGATCGCAACTTTCACAAGCTTGATCACTTCCTCTAACGAGACATTGCCGTTTGGTTTTGGTATCGGTAACGATAACCGTAACGGTAATGTTAACGTTAACGGGTTTAATGATGCGTCGTCGTTTCAGTGGCAAAACCAAAAGCTGATGAGCGTTACCGGCGATCAAAAACATCATGAAGAAGACGCATTGAAGTTGTTACCGGAGAATATAGGTGCTGGTGCTGGTGGGGGTGGTTCTTCTGGTTCTGGATCGTTGCTGGACCATGGTATTGATTTCTCTTTGCTGCAGAGTAAAACAAGCAACGGTGGATTTGGATCGTTGGATTGGAATGGTGACGGTGGTGCAGATCATCAAGGTTTGTTTGATCTTCCTAACACCGTTGATCATGGTTACTGGAATCACACTCAATGGTCTGATCAGGATCATTCTTCTCTCTTTCATCTTCCATGA

Protein sequence

>MS.gene063518.t1

MQEIQHSIGGAGGTRFFSGGGGDRRLRPNQNNNNLNQEVMKCPRCDSINTKFCYYNNYNLSQPRHFCKNCRRYWTKGGVLRNVPVGGGCRKSKRSSKPKKSSSSDSTVLPTTPPEAPENKSNSHSSSESSSLTAAAVAETVSEPTPPPEKKINKGFENDSLEQGTGDCGIFSEIATFTSLITSSNETLPFGFGIGNDNRNGNVNVNGFNDASSFQWQNQKLMSVTGDQKHHEEDALKLLPENIGAGAGGGGSSGSGSLLDHGIDFSLLQSKTSNGGFGSLDWNGDGGADHQGLFDLPNTVDHGYWNHTQWSDQDHSSLFHLP