Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064771.t1 | RHN71126.1 | 96 | 654 | 25 | 1 | 1 | 654 | 1 | 653 | 2.20E-286 | 994.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064771.t1 | P54103 | 37.4 | 625 | 288 | 17 | 62 | 653 | 55 | 609 | 9.7e-57 | 223.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064771.t1 | A0A396IZE3 | 96.0 | 654 | 25 | 1 | 1 | 654 | 1 | 653 | 1.6e-286 | 994.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene064771.t1 | TF | MYB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene064771 | 0.814479 | 1.51E-51 | -1.69E-46 |
| MS.gene049166 | MS.gene064771 | 0.839233 | 1.79E-57 | -1.69E-46 |
| MS.gene049283 | MS.gene064771 | 0.807055 | 6.09E-50 | -1.69E-46 |
| MS.gene050084 | MS.gene064771 | 0.83173 | 1.41E-55 | -1.69E-46 |
| MS.gene050085 | MS.gene064771 | 0.848842 | 4.73E-60 | -1.69E-46 |
| MS.gene050317 | MS.gene064771 | 0.807453 | 5.02E-50 | -1.69E-46 |
| MS.gene050551 | MS.gene064771 | 0.801541 | 8.58E-49 | -1.69E-46 |
| MS.gene050654 | MS.gene064771 | 0.834876 | 2.33E-56 | -1.69E-46 |
| MS.gene050689 | MS.gene064771 | 0.803775 | 2.97E-49 | -1.69E-46 |
| MS.gene050941 | MS.gene064771 | 0.80939 | 1.94E-50 | -1.69E-46 |
| MS.gene051003 | MS.gene064771 | 0.862746 | 4.07E-64 | -1.69E-46 |
| MS.gene051234 | MS.gene064771 | 0.809861 | 1.53E-50 | -1.69E-46 |
| MS.gene051343 | MS.gene064771 | 0.812746 | 3.63E-51 | -1.69E-46 |
| MS.gene051363 | MS.gene064771 | 0.818435 | 1.96E-52 | -1.69E-46 |
| MS.gene051365 | MS.gene064771 | 0.812617 | 3.87E-51 | -1.69E-46 |
| MS.gene05188 | MS.gene064771 | 0.855212 | 7.34E-62 | -1.69E-46 |
| MS.gene051975 | MS.gene064771 | 0.825192 | 5.36E-54 | -1.69E-46 |
| MS.gene052072 | MS.gene064771 | 0.807157 | 5.80E-50 | -1.69E-46 |
| MS.gene052342 | MS.gene064771 | 0.807797 | 4.24E-50 | -1.69E-46 |
| MS.gene052434 | MS.gene064771 | 0.828461 | 8.88E-55 | -1.69E-46 |
| MS.gene052518 | MS.gene064771 | 0.842811 | 2.06E-58 | -1.69E-46 |
| MS.gene053058 | MS.gene064771 | 0.822373 | 2.45E-53 | -1.69E-46 |
| MS.gene053381 | MS.gene064771 | 0.827384 | 1.61E-54 | -1.69E-46 |
| MS.gene053786 | MS.gene064771 | 0.816955 | 4.23E-52 | -1.69E-46 |
| MS.gene05517 | MS.gene064771 | 0.802844 | 4.63E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene064771 | 0.843879 | 1.07E-58 | -1.69E-46 |
| MS.gene056045 | MS.gene064771 | 0.806342 | 8.62E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene064771 | 0.838513 | 2.75E-57 | -1.69E-46 |
| MS.gene056403 | MS.gene064771 | 0.819863 | 9.29E-53 | -1.69E-46 |
| MS.gene056605 | MS.gene064771 | 0.819874 | 9.24E-53 | -1.69E-46 |
| MS.gene056786 | MS.gene064771 | 0.827342 | 1.65E-54 | -1.69E-46 |
| MS.gene056831 | MS.gene064771 | 0.842742 | 2.15E-58 | -1.69E-46 |
| MS.gene056986 | MS.gene064771 | 0.811755 | 5.97E-51 | -1.69E-46 |
| MS.gene05781 | MS.gene064771 | 0.825841 | 3.76E-54 | -1.69E-46 |
| MS.gene058292 | MS.gene064771 | 0.820113 | 8.14E-53 | -1.69E-46 |
| MS.gene058781 | MS.gene064771 | 0.814478 | 1.51E-51 | -1.69E-46 |
| MS.gene059146 | MS.gene064771 | 0.876379 | 1.46E-68 | -1.69E-46 |
| MS.gene059901 | MS.gene064771 | -0.815302 | 9.91E-52 | -1.69E-46 |
| MS.gene06010 | MS.gene064771 | 0.820672 | 6.06E-53 | -1.69E-46 |
| MS.gene06011 | MS.gene064771 | 0.873892 | 1.03E-67 | -1.69E-46 |
| MS.gene060296 | MS.gene064771 | 0.815643 | 8.32E-52 | -1.69E-46 |
| MS.gene060763 | MS.gene064771 | 0.891737 | 3.02E-74 | -1.69E-46 |
| MS.gene060830 | MS.gene064771 | 0.812233 | 4.70E-51 | -1.69E-46 |
| MS.gene060933 | MS.gene064771 | 0.805968 | 1.03E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene064771 | 0.83483 | 2.39E-56 | -1.69E-46 |
| MS.gene060937 | MS.gene064771 | 0.848536 | 5.76E-60 | -1.69E-46 |
| MS.gene061100 | MS.gene064771 | 0.830782 | 2.42E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064771.t1 | MTR_3g113680 | 97.554 | 654 | 15 | 1 | 1 | 654 | 1 | 653 | 0.0 | 1308 |
| MS.gene064771.t1 | MTR_7g109430 | 97.095 | 654 | 18 | 1 | 1 | 654 | 1 | 653 | 0.0 | 1306 |
| MS.gene064771.t1 | MTR_3g114060 | 81.720 | 279 | 43 | 2 | 378 | 654 | 62 | 334 | 1.30e-148 | 434 |
| MS.gene064771.t1 | MTR_8g093080 | 29.439 | 214 | 95 | 3 | 443 | 653 | 140 | 300 | 1.05e-20 | 93.6 |
| MS.gene064771.t1 | MTR_4g109510 | 30.417 | 240 | 115 | 9 | 98 | 300 | 9 | 233 | 3.01e-17 | 86.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064771.t1 | AT3G11450 | 63.939 | 660 | 210 | 11 | 4 | 654 | 7 | 647 | 0.0 | 734 |
| MS.gene064771.t1 | AT5G06110 | 59.492 | 669 | 239 | 10 | 2 | 651 | 5 | 660 | 0.0 | 713 |
| MS.gene064771.t1 | AT5G06110 | 59.492 | 669 | 239 | 10 | 2 | 651 | 5 | 660 | 0.0 | 713 |
| MS.gene064771.t1 | AT5G45420 | 31.959 | 194 | 87 | 3 | 459 | 652 | 158 | 306 | 7.78e-19 | 88.2 |
| MS.gene064771.t1 | AT5G45420 | 31.959 | 194 | 87 | 3 | 459 | 652 | 158 | 306 | 7.78e-19 | 88.2 |
| MS.gene064771.t1 | AT5G45420 | 31.959 | 194 | 87 | 3 | 459 | 652 | 158 | 306 | 7.78e-19 | 88.2 |
| MS.gene064771.t1 | AT1G74250 | 29.167 | 240 | 117 | 6 | 98 | 297 | 12 | 238 | 4.83e-16 | 82.4 |
Find 130 sgRNAs with CRISPR-Local
Find 155 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACTTCTTTGCCATATATT+TGG | 0.137476 | 3.4:+98000171 | None:intergenic |
| TTTCTCCTCTTCAGCAATTC+TGG | 0.180978 | 3.4:+98000132 | None:intergenic |
| GCAAGATCATTATGCATTAT+TGG | 0.181634 | 3.4:-98000824 | MS.gene064771:CDS |
| TCCTTTCTTCTCAGTATTCT+AGG | 0.194363 | 3.4:+98000229 | None:intergenic |
| CCATGATGAAGGAAAATTTC+AGG | 0.207011 | 3.4:-97999187 | MS.gene064771:CDS |
| CCATCTCCTTGAAGTTCCTT+TGG | 0.238251 | 3.4:+97999679 | None:intergenic |
| CTTTGGCTGTATCGGGCTTC+TGG | 0.244533 | 3.4:+97999582 | None:intergenic |
| CATCTCTGGTTAGCTTCCTT+TGG | 0.256785 | 3.4:+97999269 | None:intergenic |
| CAAGATCATTATGCATTATT+GGG | 0.286954 | 3.4:-98000823 | MS.gene064771:CDS |
| ATCTAATTGTCTTCCGGTTA+AGG | 0.327275 | 3.4:-98001034 | MS.gene064771:CDS |
| AGCTGAGAAGAAAAGGAAAA+AGG | 0.327832 | 3.4:-98000197 | MS.gene064771:CDS |
| ATTGTCAACTAGAGAAGAAT+TGG | 0.337451 | 3.4:-97999504 | MS.gene064771:CDS |
| TCATATTTCAACGCCTTAAC+CGG | 0.338185 | 3.4:+98001021 | None:intergenic |
| GAGAAAAGTAATCAACAAAA+TGG | 0.344685 | 3.4:-97999815 | MS.gene064771:CDS |
| CATCTCCTTGAAGTTCCTTT+GGG | 0.350239 | 3.4:+97999680 | None:intergenic |
| CTGAATCCCGAGATCATAAA+AGG | 0.353541 | 3.4:-98000348 | MS.gene064771:CDS |
| ATCTCTGGTTAGCTTCCTTT+GGG | 0.356444 | 3.4:+97999270 | None:intergenic |
| CGAGTAGCTGCAGCTGTACC+TGG | 0.359607 | 3.4:-97999242 | MS.gene064771:CDS |
| TACTCACAAGAGCTTGTAGA+TGG | 0.365954 | 3.4:-98001075 | MS.gene064771:CDS |
| TGTTTCATCTAATTGTCTTC+CGG | 0.373042 | 3.4:-98001040 | MS.gene064771:CDS |
| AATGGCTATGTCAAGGCTAA+TGG | 0.383665 | 3.4:-97999797 | MS.gene064771:CDS |
| CTTCTGTTGCAAAGCAGCTT+CGG | 0.407096 | 3.4:+98000078 | None:intergenic |
| CAGCTACTCGTTCCCATCTC+TGG | 0.408539 | 3.4:+97999255 | None:intergenic |
| AGGCAAAAGCTGAGAAGAAA+AGG | 0.413153 | 3.4:-98000204 | MS.gene064771:CDS |
| TTGTTGATAATGCTTATAAG+AGG | 0.415278 | 3.4:-98000255 | MS.gene064771:CDS |
| AGCAAGAGCAAGAAGAAATC+TGG | 0.416590 | 3.4:-98000865 | MS.gene064771:CDS |
| ATTCTTCTCTAGTTGACAAT+GGG | 0.419152 | 3.4:+97999507 | None:intergenic |
| TCATCACCTAGAGATGGAAT+TGG | 0.419175 | 3.4:+98000472 | None:intergenic |
| CCTTTCTTCTCAGTATTCTA+GGG | 0.419637 | 3.4:+98000230 | None:intergenic |
| GAAGAATGTGGTTGAAGATA+AGG | 0.419917 | 3.4:-98000920 | MS.gene064771:CDS |
| CTGCTTGTTGCTGTCGGTAC+CGG | 0.420172 | 3.4:+97999383 | None:intergenic |
| TCAAAAGCTTTGGCTGTATC+GGG | 0.425452 | 3.4:+97999575 | None:intergenic |
| CAAGACTTCTACAAGGTGTT+TGG | 0.427058 | 3.4:-98000535 | MS.gene064771:CDS |
| GCTGCTGCACTTAAACTTCT+CGG | 0.428929 | 3.4:-98000976 | MS.gene064771:CDS |
| TGATGATAAGTCTTCAATAA+AGG | 0.429949 | 3.4:-98000455 | MS.gene064771:CDS |
| AACTTCCTTGAGGTTTCATC+CGG | 0.430850 | 3.4:-98000743 | MS.gene064771:CDS |
| TTTGGTATTCCTTTAAAAGC+TGG | 0.431430 | 3.4:-98000411 | MS.gene064771:CDS |
| GAGGTTTCATCCGGACAAAC+AGG | 0.431529 | 3.4:-98000734 | MS.gene064771:CDS |
| GAGATTGAGCTATTGAGAAA+AGG | 0.438019 | 3.4:-97999716 | MS.gene064771:CDS |
| ATCAAAAGCTTTGGCTGTAT+CGG | 0.439665 | 3.4:+97999574 | None:intergenic |
| CAAATATATGGCAAAGAAGT+TGG | 0.442593 | 3.4:-98000170 | MS.gene064771:CDS |
| AAGAAATGTATCAAAAGCTT+TGG | 0.451091 | 3.4:+97999565 | None:intergenic |
| ATTTGATATCGAACAATTGA+GGG | 0.453929 | 3.4:-97999927 | MS.gene064771:CDS |
| CATTTGATATCGAACAATTG+AGG | 0.454216 | 3.4:-97999928 | MS.gene064771:CDS |
| CCAAAGGAACTTCAAGGAGA+TGG | 0.456430 | 3.4:-97999679 | MS.gene064771:CDS |
| CTTCGCAGCTTCAGTTTGCT+CGG | 0.456856 | 3.4:+98000696 | None:intergenic |
| CCAACACTGCATAAGCCTCT+TGG | 0.462398 | 3.4:+98000632 | None:intergenic |
| GATGCAATTGATTGTGCTGC+AGG | 0.463598 | 3.4:+97999530 | None:intergenic |
| ACAGCAGCAGCCTGTTTGTC+CGG | 0.463811 | 3.4:+98000724 | None:intergenic |
| CAAGATCAAACTCATCAGCC+TGG | 0.467623 | 3.4:+98000377 | None:intergenic |
| AGAGGCGTGGTCTGCAGTGC+AGG | 0.469414 | 3.4:-97999324 | MS.gene064771:CDS |
| CTGGTTAGCTTCCTTTGGGA+AGG | 0.471179 | 3.4:+97999274 | None:intergenic |
| GTCGTCGTCATCGTCGGTGT+TGG | 0.475726 | 3.4:+97999419 | None:intergenic |
| CAAAGGAAGCTAACCAGAGA+TGG | 0.482494 | 3.4:-97999268 | MS.gene064771:CDS |
| ATTGTTGTCGTCGTCATCGT+CGG | 0.494599 | 3.4:+97999413 | None:intergenic |
| TTGATACATTTCTTGAGAAG+AGG | 0.495249 | 3.4:-97999556 | MS.gene064771:CDS |
| AGCTGCTTTGCAACAGAAGA+AGG | 0.496514 | 3.4:-98000074 | MS.gene064771:CDS |
| CACTGGATCACGGTCTTCCC+AGG | 0.497559 | 3.4:+97999224 | None:intergenic |
| TCAATCTCTTCTTTAGTCCA+AGG | 0.508908 | 3.4:+97999731 | None:intergenic |
| AGCAGGCTACACAGAGAAGA+AGG | 0.509420 | 3.4:-97999759 | MS.gene064771:CDS |
| CAGAGAAGAAGGAGAAACCT+TGG | 0.509658 | 3.4:-97999748 | MS.gene064771:CDS |
| AAGATCAAACTCATCAGCCT+GGG | 0.518267 | 3.4:+98000378 | None:intergenic |
| AATTCTTCTCTAGTTGACAA+TGG | 0.519138 | 3.4:+97999506 | None:intergenic |
| CCCTAGAATACTGAGAAGAA+AGG | 0.521673 | 3.4:-98000230 | MS.gene064771:CDS |
| CCGTAGCAAGATATCTAAGA+TGG | 0.529700 | 3.4:+98000794 | None:intergenic |
| CAAAGGAACTTCAAGGAGAT+GGG | 0.529793 | 3.4:-97999678 | MS.gene064771:CDS |
| GTTGCTGTCGGTACCGGTGT+TGG | 0.530623 | 3.4:+97999389 | None:intergenic |
| GTTTCAGAATACATTGGCAC+TGG | 0.531471 | 3.4:-97999650 | MS.gene064771:CDS |
| AATCAACCAATTCCATCTCT+AGG | 0.533477 | 3.4:-98000478 | MS.gene064771:CDS |
| GCATGAAAAGAGTGACCAGC+CGG | 0.535447 | 3.4:+98000997 | None:intergenic |
| GGAAGCAGTAGTTCTCTAGC+AGG | 0.540499 | 3.4:-97999776 | MS.gene064771:CDS |
| AGCGGCCAGAATTGCTGAAG+AGG | 0.545593 | 3.4:-98000137 | MS.gene064771:CDS |
| ATCATAAAAGGTGGATGGAA+AGG | 0.545814 | 3.4:-98000336 | MS.gene064771:CDS |
| TATGGCAAAGAAGTTGGAGG+AGG | 0.545831 | 3.4:-98000164 | MS.gene064771:CDS |
| AGTTTCCCAAAGGAACTTCA+AGG | 0.546208 | 3.4:-97999685 | MS.gene064771:CDS |
| CGAGCAAACTGAAGCTGCGA+AGG | 0.546255 | 3.4:-98000695 | MS.gene064771:CDS |
| CCGAGATCATAAAAGGTGGA+TGG | 0.548047 | 3.4:-98000341 | MS.gene064771:CDS |
| TTGATGCTGCTTGTTGCTGT+CGG | 0.552607 | 3.4:+97999377 | None:intergenic |
| CCAAGAGGCTTATGCAGTGT+TGG | 0.555210 | 3.4:-98000632 | MS.gene064771:CDS |
| GCAAACTGAAGCTGCGAAGG+AGG | 0.560752 | 3.4:-98000692 | MS.gene064771:CDS |
| AATACTGAGAAGAAAGGAAG+AGG | 0.562192 | 3.4:-98000224 | MS.gene064771:CDS |
| CGTCCATTCCTCATAAAAGC+AGG | 0.567375 | 3.4:+98000511 | None:intergenic |
| TGGCTGTATCGGGCTTCTGG+AGG | 0.570452 | 3.4:+97999585 | None:intergenic |
| GACTTATCATCACCTAGAGA+TGG | 0.570557 | 3.4:+98000466 | None:intergenic |
| TATGAGGAATGGACGGTGGT+CGG | 0.570845 | 3.4:-98000503 | MS.gene064771:CDS |
| CAACTAGAGAAGAATTGGAA+GGG | 0.572911 | 3.4:-97999499 | MS.gene064771:CDS |
| AACACCTTGTAGAAGTCTTG+TGG | 0.578361 | 3.4:+98000538 | None:intergenic |
| ATCGGGCTTCTGGAGGAGAA+CGG | 0.578804 | 3.4:+97999592 | None:intergenic |
| TGTTGATAATGCTTATAAGA+GGG | 0.580470 | 3.4:-98000254 | MS.gene064771:CDS |
| GAGGTTGTTTCAGAATACAT+TGG | 0.584103 | 3.4:-97999656 | MS.gene064771:CDS |
| GGAGAAACAACGAAAAGCAG+AGG | 0.585652 | 3.4:-98000116 | MS.gene064771:CDS |
| GAAGTTGGAGGAGGAAGAAG+CGG | 0.588565 | 3.4:-98000155 | MS.gene064771:CDS |
| ATATATGGCAAAGAAGTTGG+AGG | 0.590878 | 3.4:-98000167 | MS.gene064771:CDS |
| AAAGGAAGCTAACCAGAGAT+GGG | 0.594916 | 3.4:-97999267 | MS.gene064771:CDS |
| AGCAACAAGCAGCATCAACT+CGG | 0.597280 | 3.4:-97999372 | MS.gene064771:CDS |
| CCATCTTAGATATCTTGCTA+CGG | 0.601973 | 3.4:-98000794 | MS.gene064771:CDS |
| AGCAAGAAGAAATCTGGTGA+CGG | 0.602442 | 3.4:-98000859 | MS.gene064771:CDS |
| GAGTAGCTGCAGCTGTACCT+GGG | 0.604010 | 3.4:-97999241 | MS.gene064771:CDS |
| AGTATGTGTTGTGACAAGAT+AGG | 0.605889 | 3.4:+97999989 | None:intergenic |
| TCAACAAAATGGCTATGTCA+AGG | 0.605950 | 3.4:-97999804 | MS.gene064771:CDS |
| TCAACTAGAGAAGAATTGGA+AGG | 0.608211 | 3.4:-97999500 | MS.gene064771:CDS |
| AACTAGAGAAGAATTGGAAG+GGG | 0.608691 | 3.4:-97999498 | MS.gene064771:CDS |
| ACAGAAGAAGGTGAAAGAGA+AGG | 0.608855 | 3.4:-98000062 | MS.gene064771:CDS |
| TCAAGGAGTTTCAGAACAAG+AGG | 0.609387 | 3.4:-97999342 | MS.gene064771:CDS |
| GCTATCATAAAACTTCCTTG+AGG | 0.611239 | 3.4:-98000753 | MS.gene064771:CDS |
| ACTCACAAGAGCTTGTAGAT+GGG | 0.613229 | 3.4:-98001074 | MS.gene064771:CDS |
| CATGAAAAGAGTGACCAGCC+GGG | 0.617908 | 3.4:+98000998 | None:intergenic |
| CTTGTGAGTAAGTAATAAGA+CGG | 0.621140 | 3.4:+98001088 | None:intergenic |
| GTCTTGTGGATCACAGTCAG+TGG | 0.627262 | 3.4:+98000552 | None:intergenic |
| GGAATACAAAAGTTTCCCAA+AGG | 0.628434 | 3.4:-97999695 | MS.gene064771:CDS |
| TCTTGTGGATCACAGTCAGT+GGG | 0.629359 | 3.4:+98000553 | None:intergenic |
| TAAGGCGTTGAAATATGACC+CGG | 0.631520 | 3.4:-98001016 | MS.gene064771:CDS |
| ATCAACTCGGAAGACTCTCA+AGG | 0.633952 | 3.4:-97999359 | MS.gene064771:CDS |
| AAGCTGGAGAGAGTTTCCCC+AGG | 0.640812 | 3.4:-98000395 | MS.gene064771:CDS |
| TTTGTCCGGATGAAACCTCA+AGG | 0.646808 | 3.4:+98000738 | None:intergenic |
| TTTGATATCGAACAATTGAG+GGG | 0.649974 | 3.4:-97999926 | MS.gene064771:CDS |
| GACGACAACAATTCCAACAC+CGG | 0.650077 | 3.4:-97999402 | MS.gene064771:CDS |
| TCTTAGATATCTTGCTACGG+AGG | 0.651757 | 3.4:-98000791 | MS.gene064771:CDS |
| TGATCCACAAGACTTCTACA+AGG | 0.666734 | 3.4:-98000542 | MS.gene064771:CDS |
| AGATCAAACTCATCAGCCTG+GGG | 0.670566 | 3.4:+98000379 | None:intergenic |
| GGTTTGTGTGAGAAAATCGA+AGG | 0.671097 | 3.4:-97999905 | MS.gene064771:CDS |
| GCGTTGAAATATGACCCGGC+TGG | 0.672506 | 3.4:-98001012 | MS.gene064771:CDS |
| TGAGAAAATCGAAGGCAAAG+AGG | 0.673494 | 3.4:-97999897 | MS.gene064771:CDS |
| AATCCCGAGATCATAAAAGG+TGG | 0.674327 | 3.4:-98000345 | MS.gene064771:CDS |
| TTCTTCTCTAGTTGACAATG+GGG | 0.676675 | 3.4:+97999508 | None:intergenic |
| GAGTTTCAGAACAAGAGGCG+TGG | 0.676797 | 3.4:-97999337 | MS.gene064771:CDS |
| TTGCTGCTGTAAGAATCAGA+AGG | 0.678587 | 3.4:+98000883 | None:intergenic |
| AAGCATTATCAACAAGAGTG+CGG | 0.686922 | 3.4:+98000263 | None:intergenic |
| AGGAACTTCAAGGAGATGGG+AGG | 0.689319 | 3.4:-97999675 | MS.gene064771:CDS |
| AGATGCTGATAAGAAGAATG+TGG | 0.708564 | 3.4:-98000932 | MS.gene064771:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCATAAATTCTTCTTTTTA+CGG | + | chr3.4:97999674-97999693 | None:intergenic | 15.0% |
| !! | CAATTAGATGAAACAAAAAT+AGG | + | chr3.4:97999229-97999248 | None:intergenic | 20.0% |
| !!! | TCATAAATTCTTCTTTTTAC+GGG | + | chr3.4:97999673-97999692 | None:intergenic | 20.0% |
| !!! | TGAAATAGATGTTCATTTTA+AGG | - | chr3.4:97999615-97999634 | MS.gene064771:CDS | 20.0% |
| !!! | TTGATAGTTTCTACAATTTT+TGG | - | chr3.4:97999845-97999864 | MS.gene064771:CDS | 20.0% |
| ! | AAGAAATGTATCAAAAGCTT+TGG | + | chr3.4:98000712-98000731 | None:intergenic | 25.0% |
| ! | ATTTGATATCGAACAATTGA+GGG | - | chr3.4:98000347-98000366 | MS.gene064771:CDS | 25.0% |
| ! | CAAGATCATTATGCATTATT+GGG | - | chr3.4:97999451-97999470 | MS.gene064771:CDS | 25.0% |
| ! | GAGAAAAGTAATCAACAAAA+TGG | - | chr3.4:98000459-98000478 | MS.gene064771:CDS | 25.0% |
| ! | TGATGATAAGTCTTCAATAA+AGG | - | chr3.4:97999819-97999838 | MS.gene064771:CDS | 25.0% |
| !!! | TGTTGATAATGCTTATAAGA+GGG | - | chr3.4:98000020-98000039 | MS.gene064771:CDS | 25.0% |
| !!! | TTGTTGATAATGCTTATAAG+AGG | - | chr3.4:98000019-98000038 | MS.gene064771:CDS | 25.0% |
| AATTCTTCTCTAGTTGACAA+TGG | + | chr3.4:98000771-98000790 | None:intergenic | 30.0% | |
| ATTCTTCTCTAGTTGACAAT+GGG | + | chr3.4:98000770-98000789 | None:intergenic | 30.0% | |
| ATTGTCAACTAGAGAAGAAT+TGG | - | chr3.4:98000770-98000789 | MS.gene064771:CDS | 30.0% | |
| CAAATATATGGCAAAGAAGT+TGG | - | chr3.4:98000104-98000123 | MS.gene064771:CDS | 30.0% | |
| CAACTTCTTTGCCATATATT+TGG | + | chr3.4:98000106-98000125 | None:intergenic | 30.0% | |
| CATTTGATATCGAACAATTG+AGG | - | chr3.4:98000346-98000365 | MS.gene064771:CDS | 30.0% | |
| CTTGTGAGTAAGTAATAAGA+CGG | + | chr3.4:97999189-97999208 | None:intergenic | 30.0% | |
| GAAAAAATTTGCCATGATGA+AGG | - | chr3.4:98001076-98001095 | MS.gene064771:CDS | 30.0% | |
| GAAAAAGGAGTCCAAATATA+TGG | - | chr3.4:98000092-98000111 | MS.gene064771:CDS | 30.0% | |
| GCAAGATCATTATGCATTAT+TGG | - | chr3.4:97999450-97999469 | MS.gene064771:CDS | 30.0% | |
| TGTTTCATCTAATTGTCTTC+CGG | - | chr3.4:97999234-97999253 | MS.gene064771:CDS | 30.0% | |
| TTGATACATTTCTTGAGAAG+AGG | - | chr3.4:98000718-98000737 | MS.gene064771:CDS | 30.0% | |
| TTTGATATCGAACAATTGAG+GGG | - | chr3.4:98000348-98000367 | MS.gene064771:CDS | 30.0% | |
| ! | TTTGGTATTCCTTTAAAAGC+TGG | - | chr3.4:97999863-97999882 | MS.gene064771:CDS | 30.0% |
| AACTAGAGAAGAATTGGAAG+GGG | - | chr3.4:98000776-98000795 | MS.gene064771:CDS | 35.0% | |
| AAGCATTATCAACAAGAGTG+CGG | + | chr3.4:98000014-98000033 | None:intergenic | 35.0% | |
| AATACTGAGAAGAAAGGAAG+AGG | - | chr3.4:98000050-98000069 | MS.gene064771:CDS | 35.0% | |
| AATCAACCAATTCCATCTCT+AGG | - | chr3.4:97999796-97999815 | MS.gene064771:CDS | 35.0% | |
| AGATGCTGATAAGAAGAATG+TGG | - | chr3.4:97999342-97999361 | MS.gene064771:CDS | 35.0% | |
| AGCTGAGAAGAAAAGGAAAA+AGG | - | chr3.4:98000077-98000096 | MS.gene064771:CDS | 35.0% | |
| AGTATGTGTTGTGACAAGAT+AGG | + | chr3.4:98000288-98000307 | None:intergenic | 35.0% | |
| ATATATGGCAAAGAAGTTGG+AGG | - | chr3.4:98000107-98000126 | MS.gene064771:CDS | 35.0% | |
| ATCATAAAAGGTGGATGGAA+AGG | - | chr3.4:97999938-97999957 | MS.gene064771:CDS | 35.0% | |
| ATCTAATTGTCTTCCGGTTA+AGG | - | chr3.4:97999240-97999259 | MS.gene064771:CDS | 35.0% | |
| CAAAACTGTCAGAAAAAGCT+AGG | - | chr3.4:97999968-97999987 | MS.gene064771:CDS | 35.0% | |
| CAACTAGAGAAGAATTGGAA+GGG | - | chr3.4:98000775-98000794 | MS.gene064771:CDS | 35.0% | |
| CCATCTTAGATATCTTGCTA+CGG | - | chr3.4:97999480-97999499 | MS.gene064771:CDS | 35.0% | |
| CCATGATGAAGGAAAATTTC+AGG | - | chr3.4:98001087-98001106 | MS.gene064771:CDS | 35.0% | |
| CCTTTCTTCTCAGTATTCTA+GGG | + | chr3.4:98000047-98000066 | None:intergenic | 35.0% | |
| GAAGAATGTGGTTGAAGATA+AGG | - | chr3.4:97999354-97999373 | MS.gene064771:CDS | 35.0% | |
| GAGATTGAGCTATTGAGAAA+AGG | - | chr3.4:98000558-98000577 | MS.gene064771:CDS | 35.0% | |
| GCTATCATAAAACTTCCTTG+AGG | - | chr3.4:97999521-97999540 | MS.gene064771:CDS | 35.0% | |
| GGAATACAAAAGTTTCCCAA+AGG | - | chr3.4:98000579-98000598 | MS.gene064771:CDS | 35.0% | |
| TCAACAAAATGGCTATGTCA+AGG | - | chr3.4:98000470-98000489 | MS.gene064771:CDS | 35.0% | |
| TCAACTAGAGAAGAATTGGA+AGG | - | chr3.4:98000774-98000793 | MS.gene064771:CDS | 35.0% | |
| TCAATCTCTTCTTTAGTCCA+AGG | + | chr3.4:98000546-98000565 | None:intergenic | 35.0% | |
| TCATATTTCAACGCCTTAAC+CGG | + | chr3.4:97999256-97999275 | None:intergenic | 35.0% | |
| TCCTTTCTTCTCAGTATTCT+AGG | + | chr3.4:98000048-98000067 | None:intergenic | 35.0% | |
| TTCTTCTCTAGTTGACAATG+GGG | + | chr3.4:98000769-98000788 | None:intergenic | 35.0% | |
| ! | AAACTCTCTCCAGCTTTTAA+AGG | + | chr3.4:97999875-97999894 | None:intergenic | 35.0% |
| ! | ATCAAAAGCTTTGGCTGTAT+CGG | + | chr3.4:98000703-98000722 | None:intergenic | 35.0% |
| ! | CCTGAAATTTTCCTTCATCA+TGG | + | chr3.4:98001090-98001109 | None:intergenic | 35.0% |
| ! | GAGGTTGTTTCAGAATACAT+TGG | - | chr3.4:98000618-98000637 | MS.gene064771:CDS | 35.0% |
| !! | AGCTTTAAAAACCTTCCCAA+AGG | - | chr3.4:98000989-98001008 | MS.gene064771:CDS | 35.0% |
| !! | CTATTTTCAGTTGTTGTTGC+TGG | + | chr3.4:98000810-98000829 | None:intergenic | 35.0% |
| !!! | TCATTTTAAGGCAATCCAAG+AGG | - | chr3.4:97999627-97999646 | MS.gene064771:CDS | 35.0% |
| !!! | TGGCAAATTTTTTCTTGCAC+TGG | + | chr3.4:98001070-98001089 | None:intergenic | 35.0% |
| !!! | TTTTTTCTTGCACTGGATCA+CGG | + | chr3.4:98001063-98001082 | None:intergenic | 35.0% |
| AAAGGAAGCTAACCAGAGAT+GGG | - | chr3.4:98001007-98001026 | MS.gene064771:CDS | 40.0% | |
| AACACCTTGTAGAAGTCTTG+TGG | + | chr3.4:97999739-97999758 | None:intergenic | 40.0% | |
| AACTTCCTTGAGGTTTCATC+CGG | - | chr3.4:97999531-97999550 | MS.gene064771:CDS | 40.0% | |
| AAGATCAAACTCATCAGCCT+GGG | + | chr3.4:97999899-97999918 | None:intergenic | 40.0% | |
| AATCCCGAGATCATAAAAGG+TGG | - | chr3.4:97999929-97999948 | MS.gene064771:CDS | 40.0% | |
| AATGGCTATGTCAAGGCTAA+TGG | - | chr3.4:98000477-98000496 | MS.gene064771:CDS | 40.0% | |
| ACAGAAGAAGGTGAAAGAGA+AGG | - | chr3.4:98000212-98000231 | MS.gene064771:CDS | 40.0% | |
| ACTCACAAGAGCTTGTAGAT+GGG | - | chr3.4:97999200-97999219 | MS.gene064771:CDS | 40.0% | |
| ACTGAGTTGCAAGAAAAACG+TGG | - | chr3.4:98000431-98000450 | MS.gene064771:CDS | 40.0% | |
| ACTGTCAGAAAAAGCTAGGA+AGG | - | chr3.4:97999972-97999991 | MS.gene064771:CDS | 40.0% | |
| AGAAGGAGAAAAAGCTCTTG+CGG | - | chr3.4:98000229-98000248 | MS.gene064771:CDS | 40.0% | |
| AGCAAGAAGAAATCTGGTGA+CGG | - | chr3.4:97999415-97999434 | MS.gene064771:CDS | 40.0% | |
| AGCAAGAGCAAGAAGAAATC+TGG | - | chr3.4:97999409-97999428 | MS.gene064771:CDS | 40.0% | |
| AGGCAAAAGCTGAGAAGAAA+AGG | - | chr3.4:98000070-98000089 | MS.gene064771:CDS | 40.0% | |
| AGTTTCCCAAAGGAACTTCA+AGG | - | chr3.4:98000589-98000608 | MS.gene064771:CDS | 40.0% | |
| CAAAGGAACTTCAAGGAGAT+GGG | - | chr3.4:98000596-98000615 | MS.gene064771:CDS | 40.0% | |
| CATCTCCTTGAAGTTCCTTT+GGG | + | chr3.4:98000597-98000616 | None:intergenic | 40.0% | |
| CCCTAGAATACTGAGAAGAA+AGG | - | chr3.4:98000044-98000063 | MS.gene064771:CDS | 40.0% | |
| CCGTAGCAAGATATCTAAGA+TGG | + | chr3.4:97999483-97999502 | None:intergenic | 40.0% | |
| CTGAATCCCGAGATCATAAA+AGG | - | chr3.4:97999926-97999945 | MS.gene064771:CDS | 40.0% | |
| GACTTATCATCACCTAGAGA+TGG | + | chr3.4:97999811-97999830 | None:intergenic | 40.0% | |
| GGTTTGTGTGAGAAAATCGA+AGG | - | chr3.4:98000369-98000388 | MS.gene064771:CDS | 40.0% | |
| GTTTCAGAATACATTGGCAC+TGG | - | chr3.4:98000624-98000643 | MS.gene064771:CDS | 40.0% | |
| TACTCACAAGAGCTTGTAGA+TGG | - | chr3.4:97999199-97999218 | MS.gene064771:CDS | 40.0% | |
| TCAAGGAGTTTCAGAACAAG+AGG | - | chr3.4:98000932-98000951 | MS.gene064771:CDS | 40.0% | |
| TCATCACCTAGAGATGGAAT+TGG | + | chr3.4:97999805-97999824 | None:intergenic | 40.0% | |
| TCTTAGATATCTTGCTACGG+AGG | - | chr3.4:97999483-97999502 | MS.gene064771:CDS | 40.0% | |
| TGAGAAAATCGAAGGCAAAG+AGG | - | chr3.4:98000377-98000396 | MS.gene064771:CDS | 40.0% | |
| TGATCCACAAGACTTCTACA+AGG | - | chr3.4:97999732-97999751 | MS.gene064771:CDS | 40.0% | |
| TTGCTGCTGTAAGAATCAGA+AGG | + | chr3.4:97999394-97999413 | None:intergenic | 40.0% | |
| TTTCTCCTCTTCAGCAATTC+TGG | + | chr3.4:98000145-98000164 | None:intergenic | 40.0% | |
| ! | CAAGACTTCTACAAGGTGTT+TGG | - | chr3.4:97999739-97999758 | MS.gene064771:CDS | 40.0% |
| ! | CATCCACCTTTTATGATCTC+GGG | + | chr3.4:97999935-97999954 | None:intergenic | 40.0% |
| ! | CCATCCACCTTTTATGATCT+CGG | + | chr3.4:97999936-97999955 | None:intergenic | 40.0% |
| ! | TCAAAAGCTTTGGCTGTATC+GGG | + | chr3.4:98000702-98000721 | None:intergenic | 40.0% |
| !! | ATCTCTGGTTAGCTTCCTTT+GGG | + | chr3.4:98001007-98001026 | None:intergenic | 40.0% |
| !! | CTGCTTTTATGAGGAATGGA+CGG | - | chr3.4:97999764-97999783 | MS.gene064771:CDS | 40.0% |
| !! | TAAGGCGTTGAAATATGACC+CGG | - | chr3.4:97999258-97999277 | MS.gene064771:CDS | 40.0% |
| !! | TGTTTGGTCCTGCTTTTATG+AGG | - | chr3.4:97999755-97999774 | MS.gene064771:CDS | 40.0% |
| AGATCAAACTCATCAGCCTG+GGG | + | chr3.4:97999898-97999917 | None:intergenic | 45.0% | |
| AGCAACAAGCAGCATCAACT+CGG | - | chr3.4:98000902-98000921 | MS.gene064771:CDS | 45.0% | |
| ATCAACTCGGAAGACTCTCA+AGG | - | chr3.4:98000915-98000934 | MS.gene064771:CDS | 45.0% | |
| ATTGTTGTCGTCGTCATCGT+CGG | + | chr3.4:98000864-98000883 | None:intergenic | 45.0% | |
| CAAAGGAAGCTAACCAGAGA+TGG | - | chr3.4:98001006-98001025 | MS.gene064771:CDS | 45.0% | |
| CAAGATCAAACTCATCAGCC+TGG | + | chr3.4:97999900-97999919 | None:intergenic | 45.0% | |
| CAGAGAAGAAGGAGAAACCT+TGG | - | chr3.4:98000526-98000545 | MS.gene064771:CDS | 45.0% | |
| CCAAAGGAACTTCAAGGAGA+TGG | - | chr3.4:98000595-98000614 | MS.gene064771:CDS | 45.0% | |
| CCATCTCCTTGAAGTTCCTT+TGG | + | chr3.4:98000598-98000617 | None:intergenic | 45.0% | |
| CCGAGATCATAAAAGGTGGA+TGG | - | chr3.4:97999933-97999952 | MS.gene064771:CDS | 45.0% | |
| CGTCCATTCCTCATAAAAGC+AGG | + | chr3.4:97999766-97999785 | None:intergenic | 45.0% | |
| CTTCTGTTGCAAAGCAGCTT+CGG | + | chr3.4:98000199-98000218 | None:intergenic | 45.0% | |
| GACGACAACAATTCCAACAC+CGG | - | chr3.4:98000872-98000891 | MS.gene064771:CDS | 45.0% | |
| GATGCAATTGATTGTGCTGC+AGG | + | chr3.4:98000747-98000766 | None:intergenic | 45.0% | |
| GCTGCTGCACTTAAACTTCT+CGG | - | chr3.4:97999298-97999317 | MS.gene064771:CDS | 45.0% | |
| GGAGAAACAACGAAAAGCAG+AGG | - | chr3.4:98000158-98000177 | MS.gene064771:CDS | 45.0% | |
| TATGGCAAAGAAGTTGGAGG+AGG | - | chr3.4:98000110-98000129 | MS.gene064771:CDS | 45.0% | |
| TCTTGTGGATCACAGTCAGT+GGG | + | chr3.4:97999724-97999743 | None:intergenic | 45.0% | |
| TTTGTCCGGATGAAACCTCA+AGG | + | chr3.4:97999539-97999558 | None:intergenic | 45.0% | |
| ! | AGCTGCTTTGCAACAGAAGA+AGG | - | chr3.4:98000200-98000219 | MS.gene064771:CDS | 45.0% |
| ! | CTTTTATGAGGAATGGACGG+TGG | - | chr3.4:97999767-97999786 | MS.gene064771:CDS | 45.0% |
| ! | TTGATGCTGCTTGTTGCTGT+CGG | + | chr3.4:98000900-98000919 | None:intergenic | 45.0% |
| !! | CATCTCTGGTTAGCTTCCTT+TGG | + | chr3.4:98001008-98001027 | None:intergenic | 45.0% |
| !! | GGTCCTGCTTTTATGAGGAA+TGG | - | chr3.4:97999760-97999779 | MS.gene064771:CDS | 45.0% |
| AGCAGGCTACACAGAGAAGA+AGG | - | chr3.4:98000515-98000534 | MS.gene064771:CDS | 50.0% | |
| AGGAACTTCAAGGAGATGGG+AGG | - | chr3.4:98000599-98000618 | MS.gene064771:CDS | 50.0% | |
| CATGAAAAGAGTGACCAGCC+GGG | + | chr3.4:97999279-97999298 | None:intergenic | 50.0% | |
| CCAACACTGCATAAGCCTCT+TGG | + | chr3.4:97999645-97999664 | None:intergenic | 50.0% | |
| CCAAGAGGCTTATGCAGTGT+TGG | - | chr3.4:97999642-97999661 | MS.gene064771:CDS | 50.0% | |
| GAAGTTGGAGGAGGAAGAAG+CGG | - | chr3.4:98000119-98000138 | MS.gene064771:CDS | 50.0% | |
| GAGGTTTCATCCGGACAAAC+AGG | - | chr3.4:97999540-97999559 | MS.gene064771:CDS | 50.0% | |
| GAGTTTCAGAACAAGAGGCG+TGG | - | chr3.4:98000937-98000956 | MS.gene064771:CDS | 50.0% | |
| GCATGAAAAGAGTGACCAGC+CGG | + | chr3.4:97999280-97999299 | None:intergenic | 50.0% | |
| GGAAGCAGTAGTTCTCTAGC+AGG | - | chr3.4:98000498-98000517 | MS.gene064771:CDS | 50.0% | |
| GGAGAAAAAGCTCTTGCGGA+AGG | - | chr3.4:98000233-98000252 | MS.gene064771:CDS | 50.0% | |
| GTCTTGTGGATCACAGTCAG+TGG | + | chr3.4:97999725-97999744 | None:intergenic | 50.0% | |
| TATGAGGAATGGACGGTGGT+CGG | - | chr3.4:97999771-97999790 | MS.gene064771:CDS | 50.0% | |
| ! | CTTCGCAGCTTCAGTTTGCT+CGG | + | chr3.4:97999581-97999600 | None:intergenic | 50.0% |
| !! | CTGGTTAGCTTCCTTTGGGA+AGG | + | chr3.4:98001003-98001022 | None:intergenic | 50.0% |
| AAGCTGGAGAGAGTTTCCCC+AGG | - | chr3.4:97999879-97999898 | MS.gene064771:CDS | 55.0% | |
| ACAGCAGCAGCCTGTTTGTC+CGG | + | chr3.4:97999553-97999572 | None:intergenic | 55.0% | |
| AGCGGCCAGAATTGCTGAAG+AGG | - | chr3.4:98000137-98000156 | MS.gene064771:CDS | 55.0% | |
| CAGCTACTCGTTCCCATCTC+TGG | + | chr3.4:98001022-98001041 | None:intergenic | 55.0% | |
| CGAGCAAACTGAAGCTGCGA+AGG | - | chr3.4:97999579-97999598 | MS.gene064771:CDS | 55.0% | |
| CTTTGGCTGTATCGGGCTTC+TGG | + | chr3.4:98000695-98000714 | None:intergenic | 55.0% | |
| GAGTAGCTGCAGCTGTACCT+GGG | - | chr3.4:98001033-98001052 | MS.gene064771:CDS | 55.0% | |
| GCAAACTGAAGCTGCGAAGG+AGG | - | chr3.4:97999582-97999601 | MS.gene064771:CDS | 55.0% | |
| GCGTTGAAATATGACCCGGC+TGG | - | chr3.4:97999262-97999281 | MS.gene064771:CDS | 55.0% | |
| ! | ATCGGGCTTCTGGAGGAGAA+CGG | + | chr3.4:98000685-98000704 | None:intergenic | 55.0% |
| ! | CTGCTTGTTGCTGTCGGTAC+CGG | + | chr3.4:98000894-98000913 | None:intergenic | 55.0% |
| CACTGGATCACGGTCTTCCC+AGG | + | chr3.4:98001053-98001072 | None:intergenic | 60.0% | |
| CGAGTAGCTGCAGCTGTACC+TGG | - | chr3.4:98001032-98001051 | MS.gene064771:CDS | 60.0% | |
| GTCGTCGTCATCGTCGGTGT+TGG | + | chr3.4:98000858-98000877 | None:intergenic | 60.0% | |
| TGGCTGTATCGGGCTTCTGG+AGG | + | chr3.4:98000692-98000711 | None:intergenic | 60.0% | |
| !! | GTTGCTGTCGGTACCGGTGT+TGG | + | chr3.4:98000888-98000907 | None:intergenic | 60.0% |
| ! | AGAGGCGTGGTCTGCAGTGC+AGG | - | chr3.4:98000950-98000969 | MS.gene064771:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 97999166 | 98001130 | 97999166 | ID=MS.gene064771 |
| chr3.4 | mRNA | 97999166 | 98001130 | 97999166 | ID=MS.gene064771.t1;Parent=MS.gene064771 |
| chr3.4 | exon | 97999166 | 98001130 | 97999166 | ID=MS.gene064771.t1.exon1;Parent=MS.gene064771.t1 |
| chr3.4 | CDS | 97999166 | 98001130 | 97999166 | ID=cds.MS.gene064771.t1;Parent=MS.gene064771.t1 |
| Gene Sequence |
| Protein sequence |
