AlfalfaGEDB | MS.gene068503

M. sativa cultivar XinJiangDaYe / MS.gene068503

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068503.t1	KEH15888.1	68.9	151	36	1	19	169	154	293	8.50E-57	229.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068503.t1	A0A072TG10	68.9	151	36	1	19	169	154	293	6.1e-57	229.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene068503	0.806606	7.58E-50	-1.69E-46
MS.gene049850	MS.gene068503	0.8137	2.24E-51	-1.69E-46
MS.gene050156	MS.gene068503	0.838814	2.30E-57	-1.69E-46
MS.gene050780	MS.gene068503	0.801109	1.05E-48	-1.69E-46
MS.gene050844	MS.gene068503	0.803186	3.93E-49	-1.69E-46
MS.gene051247	MS.gene068503	0.821102	4.83E-53	-1.69E-46
MS.gene051719	MS.gene068503	0.801534	8.61E-49	-1.69E-46
MS.gene051744	MS.gene068503	0.806399	8.38E-50	-1.69E-46
MS.gene052519	MS.gene068503	0.80112	1.05E-48	-1.69E-46
MS.gene052611	MS.gene068503	0.803616	3.20E-49	-1.69E-46
MS.gene052773	MS.gene068503	0.814885	1.23E-51	-1.69E-46
MS.gene052863	MS.gene068503	0.811396	7.15E-51	-1.69E-46
MS.gene052896	MS.gene068503	0.805487	1.30E-49	-1.69E-46
MS.gene053226	MS.gene068503	0.812198	4.78E-51	-1.69E-46
MS.gene053966	MS.gene068503	0.800307	1.53E-48	-1.69E-46
MS.gene05415	MS.gene068503	0.818812	1.61E-52	-1.69E-46
MS.gene054291	MS.gene068503	-0.805206	1.49E-49	-1.69E-46
MS.gene054467	MS.gene068503	0.80144	8.99E-49	-1.69E-46
MS.gene054468	MS.gene068503	0.801224	9.96E-49	-1.69E-46
MS.gene055466	MS.gene068503	0.807763	4.31E-50	-1.69E-46
MS.gene055818	MS.gene068503	0.856657	2.77E-62	-1.69E-46
MS.gene05604	MS.gene068503	0.801419	9.08E-49	-1.69E-46
MS.gene056050	MS.gene068503	0.809699	1.66E-50	-1.69E-46
MS.gene056366	MS.gene068503	0.809177	2.15E-50	-1.69E-46
MS.gene056498	MS.gene068503	0.804521	2.08E-49	-1.69E-46
MS.gene057026	MS.gene068503	0.818946	1.50E-52	-1.69E-46
MS.gene057060	MS.gene068503	0.800168	1.64E-48	-1.69E-46
MS.gene057444	MS.gene068503	0.817167	3.79E-52	-1.69E-46
MS.gene057598	MS.gene068503	0.804067	2.58E-49	-1.69E-46
MS.gene057915	MS.gene068503	0.803696	3.08E-49	-1.69E-46
MS.gene058625	MS.gene068503	0.803576	3.26E-49	-1.69E-46
MS.gene058636	MS.gene068503	0.82434	8.51E-54	-1.69E-46
MS.gene058829	MS.gene068503	0.836534	8.85E-57	-1.69E-46
MS.gene058979	MS.gene068503	0.803025	4.24E-49	-1.69E-46
MS.gene060322	MS.gene068503	0.800973	1.12E-48	-1.69E-46
MS.gene060521	MS.gene068503	0.803407	3.54E-49	-1.69E-46
MS.gene060572	MS.gene068503	0.812678	3.76E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068503.t1	MTR_0462s0020	68.874	151	36	1	19	169	154	293	3.76e-76	229
MS.gene068503.t1	MTR_2g092960	59.060	149	49	2	19	167	1079	1215	5.85e-53	181
MS.gene068503.t1	MTR_8g017000	49.080	163	71	2	6	167	534	685	9.32e-47	162
MS.gene068503.t1	MTR_2g037270	48.466	163	72	2	6	167	534	685	4.41e-46	160
MS.gene068503.t1	MTR_4g048180	53.642	151	21	2	19	169	170	271	3.34e-44	147
MS.gene068503.t1	MTR_3g011560	45.399	163	64	2	6	167	534	672	7.27e-42	148
MS.gene068503.t1	MTR_5g077900	36.054	147	81	1	21	167	89	222	1.87e-25	97.8
MS.gene068503.t1	MTR_0002s1270	36.301	146	80	1	21	166	263	395	1.44e-24	98.6
MS.gene068503.t1	MTR_7g046380	36.957	138	74	1	21	158	271	395	5.30e-24	97.1
MS.gene068503.t1	MTR_3g052060	72.093	43	12	0	127	169	1	43	4.25e-19	77.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 26 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATAACCGCAAAGCTGTTCC+AGG	0.373449	4.2:-57975135	MS.gene068503:CDS
ATAATTTCGTCATCAGTAAA+CGG	0.378546	4.2:+57974347	None:intergenic
CAGCTGGTATTACTGATTCA+TGG	0.413545	4.2:-57974474	MS.gene068503:CDS
TCAAATGCACTTACTTTCTC+AGG	0.418722	4.2:+57975084	None:intergenic
TAATTTCGTCATCAGTAAAC+GGG	0.428884	4.2:+57974348	None:intergenic
TATGAGACTGAGAACAATCT+TGG	0.430936	4.2:+57975154	None:intergenic
TATTATTGTAGTGCTATTGA+AGG	0.459831	4.2:-57974751	MS.gene068503:intron
GTATCCCACGATGGCTTTCT+CGG	0.461717	4.2:+57974667	None:intergenic
TATTACTGATTCATGGGATC+TGG	0.464985	4.2:-57974467	MS.gene068503:intron
TGCACTTACTTTCTCAGGTA+AGG	0.468229	4.2:+57975089	None:intergenic
TTTACCCTGGAACAGCTTTG+CGG	0.476816	4.2:+57975130	None:intergenic
GTATAATAAGTTGAGCGGTT+TGG	0.476911	4.2:-57974728	MS.gene068503:CDS
ATGTTCGATGGGGTGGAAGC+CGG	0.483605	4.2:-57974917	MS.gene068503:CDS
TTTCCAAATGTAGTCGCCAT+TGG	0.490741	4.2:-57974984	MS.gene068503:intron
AGCTGGTATTACTGATTCAT+GGG	0.511132	4.2:-57974473	MS.gene068503:CDS
ATGTTGAACGTTGTATCAGC+TGG	0.543506	4.2:-57974490	MS.gene068503:CDS
ATTATTGTAGTGCTATTGAA+GGG	0.549725	4.2:-57974750	MS.gene068503:intron
GCTTCCACCCCATCGAACAT+AGG	0.550012	4.2:+57974920	None:intergenic
GAAGGGTATAATAAGTTGAG+CGG	0.607687	4.2:-57974733	MS.gene068503:CDS
CAATAATGAGCAATTGCCAA+TGG	0.614170	4.2:+57974968	None:intergenic
TAGCCGCACTCATAATTGAA+CGG	0.655981	4.2:+57974529	None:intergenic
ATAACCGCAAAGCTGTTCCA+GGG	0.658973	4.2:-57975134	MS.gene068503:CDS
CAACCGTTCAATTATGAGTG+CGG	0.663655	4.2:-57974532	MS.gene068503:CDS
GAAAGCCGAGAAAGCCATCG+TGG	0.664822	4.2:-57974672	MS.gene068503:CDS
AAAGCCGAGAAAGCCATCGT+GGG	0.675119	4.2:-57974671	MS.gene068503:CDS
ACTGTGGGAGTATCCCACGA+TGG	0.746249	4.2:+57974658	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATTAATCACCTATATAT+TGG	-	chr4.2:57974456-57974475	MS.gene068503:intron	15.0%
!!!	AAAACTACATTTTGTTTTTT+TGG	+	chr4.2:57974555-57974574	None:intergenic	15.0%
!!!	ATATTATTTTTAACTTACTG+TGG	+	chr4.2:57974856-57974875	None:intergenic	15.0%
!!!	TAATATGTTTTTTTTTCTTC+AGG	-	chr4.2:57975108-57975127	MS.gene068503:CDS	15.0%
!!!	TATTATTTTTAACTTACTGT+GGG	+	chr4.2:57974855-57974874	None:intergenic	15.0%
!!	CAAAAAAAAAAAAAGTTGAG+CGG	-	chr4.2:57974795-57974814	MS.gene068503:intron	20.0%
!!	TCTTGTAAAAAAATCAAGTT+GGG	+	chr4.2:57974704-57974723	None:intergenic	20.0%
!!	TTCAATTCTTTCCAATATAT+AGG	+	chr4.2:57974470-57974489	None:intergenic	20.0%
!	ACACTACAATTGTCAAAAAT+AGG	+	chr4.2:57974937-57974956	None:intergenic	25.0%
!	ATAATTTCGTCATCAGTAAA+CGG	+	chr4.2:57975151-57975170	None:intergenic	25.0%
!	GTCTTGTAAAAAAATCAAGT+TGG	+	chr4.2:57974705-57974724	None:intergenic	25.0%
!!	ATTATTGTAGTGCTATTGAA+GGG	-	chr4.2:57974745-57974764	MS.gene068503:intron	25.0%
!!	TATTATTGTAGTGCTATTGA+AGG	-	chr4.2:57974744-57974763	MS.gene068503:intron	25.0%
!!!	AATAACAAACTTTTTTACCC+TGG	+	chr4.2:57974381-57974400	None:intergenic	25.0%
!!!	TTTTTTTTTCTTCAGGTCTT+TGG	-	chr4.2:57975115-57975134	MS.gene068503:CDS	25.0%
	TAATTTCGTCATCAGTAAAC+GGG	+	chr4.2:57975150-57975169	None:intergenic	30.0%
!	TTTTCTGCGTGAATGAAAAA+TGG	+	chr4.2:57974318-57974337	None:intergenic	30.0%
!!	ATGTGCTCGATATTTTTATC+CGG	+	chr4.2:57974600-57974619	None:intergenic	30.0%
	CAATAATGAGCAATTGCCAA+TGG	+	chr4.2:57974530-57974549	None:intergenic	35.0%
	TATGAGACTGAGAACAATCT+TGG	+	chr4.2:57974344-57974363	None:intergenic	35.0%
	TCAAATGCACTTACTTTCTC+AGG	+	chr4.2:57974414-57974433	None:intergenic	35.0%
!	GAAGGGTATAATAAGTTGAG+CGG	-	chr4.2:57974762-57974781	MS.gene068503:intron	35.0%
!	GTAGTTTTCCTATGTTCGAT+GGG	-	chr4.2:57974567-57974586	MS.gene068503:intron	35.0%
!	GTATAATAAGTTGAGCGGTT+TGG	-	chr4.2:57974767-57974786	MS.gene068503:intron	35.0%
!	GTTGTATTTTGTACTGCTGA+AGG	-	chr4.2:57974659-57974678	MS.gene068503:intron	35.0%
!	TAGTTTTCCTATGTTCGATG+GGG	-	chr4.2:57974568-57974587	MS.gene068503:intron	35.0%
!	TGTAGTTTTCCTATGTTCGA+TGG	-	chr4.2:57974566-57974585	MS.gene068503:intron	35.0%
!!	AGCTGGTATTACTGATTCAT+GGG	-	chr4.2:57975022-57975041	MS.gene068503:intron	35.0%
!!	TATTACTGATTCATGGGATC+TGG	-	chr4.2:57975028-57975047	MS.gene068503:intron	35.0%
	ATGTTGAACGTTGTATCAGC+TGG	-	chr4.2:57975005-57975024	MS.gene068503:intron	40.0%
	CAACCGTTCAATTATGAGTG+CGG	-	chr4.2:57974963-57974982	MS.gene068503:CDS	40.0%
	TAGCCGCACTCATAATTGAA+CGG	+	chr4.2:57974969-57974988	None:intergenic	40.0%
	TGCACTTACTTTCTCAGGTA+AGG	+	chr4.2:57974409-57974428	None:intergenic	40.0%
	TTTCCAAATGTAGTCGCCAT+TGG	-	chr4.2:57974511-57974530	MS.gene068503:CDS	40.0%
!!	CAGCTGGTATTACTGATTCA+TGG	-	chr4.2:57975021-57975040	MS.gene068503:intron	40.0%
	ATAACCGCAAAGCTGTTCCA+GGG	-	chr4.2:57974361-57974380	MS.gene068503:CDS	45.0%
	TGCTGAAGGCTCTTTACTCT+GGG	-	chr4.2:57974673-57974692	MS.gene068503:CDS	45.0%
	TTGCCAATGGCGACTACATT+TGG	+	chr4.2:57974517-57974536	None:intergenic	45.0%
	TTTACCCTGGAACAGCTTTG+CGG	+	chr4.2:57974368-57974387	None:intergenic	45.0%
!	TTTTCCTATGTTCGATGGGG+TGG	-	chr4.2:57974571-57974590	MS.gene068503:intron	45.0%
	AAAGCCGAGAAAGCCATCGT+GGG	-	chr4.2:57974824-57974843	MS.gene068503:intron	50.0%
	CATAACCGCAAAGCTGTTCC+AGG	-	chr4.2:57974360-57974379	MS.gene068503:CDS	50.0%
	CTGCTGAAGGCTCTTTACTC+TGG	-	chr4.2:57974672-57974691	MS.gene068503:CDS	50.0%
	GTATCCCACGATGGCTTTCT+CGG	+	chr4.2:57974831-57974850	None:intergenic	50.0%
	ATGTTCGATGGGGTGGAAGC+CGG	-	chr4.2:57974578-57974597	MS.gene068503:intron	55.0%
	GAAAGCCGAGAAAGCCATCG+TGG	-	chr4.2:57974823-57974842	MS.gene068503:intron	55.0%
	GCTTCCACCCCATCGAACAT+AGG	+	chr4.2:57974578-57974597	None:intergenic	55.0%
!!	ACTGTGGGAGTATCCCACGA+TGG	+	chr4.2:57974840-57974859	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	57974301	57975216	57974301	ID=MS.gene068503
chr4.2	mRNA	57974301	57975216	57974301	ID=MS.gene068503.t1;Parent=MS.gene068503
chr4.2	exon	57975098	57975216	57975098	ID=MS.gene068503.t1.exon1;Parent=MS.gene068503.t1
chr4.2	CDS	57975098	57975216	57975098	ID=cds.MS.gene068503.t1;Parent=MS.gene068503.t1
chr4.2	exon	57974889	57974993	57974889	ID=MS.gene068503.t1.exon2;Parent=MS.gene068503.t1
chr4.2	CDS	57974889	57974993	57974889	ID=cds.MS.gene068503.t1;Parent=MS.gene068503.t1
chr4.2	exon	57974660	57974762	57974660	ID=MS.gene068503.t1.exon3;Parent=MS.gene068503.t1
chr4.2	CDS	57974660	57974762	57974660	ID=cds.MS.gene068503.t1;Parent=MS.gene068503.t1
chr4.2	exon	57974468	57974563	57974468	ID=MS.gene068503.t1.exon4;Parent=MS.gene068503.t1
chr4.2	CDS	57974468	57974563	57974468	ID=cds.MS.gene068503.t1;Parent=MS.gene068503.t1
chr4.2	exon	57974301	57974387	57974301	ID=MS.gene068503.t1.exon5;Parent=MS.gene068503.t1
chr4.2	CDS	57974301	57974387	57974301	ID=cds.MS.gene068503.t1;Parent=MS.gene068503.t1

Gene Sequence

>MS.gene068503

ATGGGTTCGTTGATCCATTTTTCATTCACGCAGAAAATAACCAAGATTGTTCTCAGTCTCATAACCGCAAAGCTGTTCCAGGGTAAAAAAGTTTGTTATTTTGCACCTTACCTGAGAAAGTAAGTGCATTTGATCTTCAGAGTAATTTTTTTGCTATAATTAATCACCTATATATTGGAAAGAATTGAATTTAACTAATTTAATTTTTTGTTTCCAAATGTAGTCGCCATTGGCAATTGCTCATTATTGAACCAAAAAAACAAAATGTAGTTTTCCTATGTTCGATGGGGTGGAAGCCGGATAAAAATATCGAGCACATTGTTAATTCGTAAGTATTTAACTTGTGAAATTTAATTACGTTGTATTTTGTACTGCTGAAGGCTCTTTACTCTGGGTTATTCCCAACTTGATTTTTTTACAAGACAATTTTAATGAATATATTTTATTATTGTAGTGCTATTGAAGGGTATAATAAGTTGAGCGGTTTGGTCTATCAAAAAAAAAAAAAGTTGAGCGGTTTGAGAAAGCCGAGAAAGCCATCGTGGGATACTCCCACAGTAAGTTAAAAATAATATTTGAAGAAATTCATATAGTAATATAAGTCTTATATATATAATATTTACTCAATAATTTCCTATTTTTGACAATTGTAGTGTCAAAGACAACCGTTCAATTATGAGTGCGGCTATTATATCATGATACATATGTTGAACGTTGTATCAGCTGGTATTACTGATTCATGGGATCTGGTGCGTAATTGAACATTTCAATCATTTGTGTTTTTTTAAGTTAATATGATATTATAATTAATATGTTTTTTTTTCTTCAGGTCTTTGGAGACAAAAACCCGTTTACTGATGACGAAATTATGAATGTTCAAGAACGTTGTGCAAGTTTGATTCTGCAAAGACTTTGA

Protein sequence

>MS.gene068503.t1

MGSLIHFSFTQKITKIVLSLITAKLFQGKKVCYFAPYLRNRHWQLLIIEPKKQNVVFLCSMGWKPDKNIEHIVNSAIEGYNKLSGLVYQKKKKLSGLRKPRKPSWDTPTCQRQPFNYECGYYIMIHMLNVVSAGITDSWDLVFGDKNPFTDDEIMNVQERCASLILQRL