Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068950.t1 | XP_003628404.1 | 95.4 | 219 | 10 | 0 | 1 | 219 | 1 | 219 | 3.80E-118 | 434.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068950.t1 | P49332 | 68.3 | 218 | 68 | 1 | 3 | 219 | 4 | 221 | 2.2e-82 | 306.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068950.t1 | G7LB15 | 95.4 | 219 | 10 | 0 | 1 | 219 | 1 | 219 | 2.8e-118 | 434.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene068950 | 0.805652 | 1.20E-49 | -1.69E-46 |
| MS.gene049233 | MS.gene068950 | 0.818733 | 1.68E-52 | -1.69E-46 |
| MS.gene049260 | MS.gene068950 | 0.802119 | 6.53E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene068950 | 0.815868 | 7.41E-52 | -1.69E-46 |
| MS.gene050227 | MS.gene068950 | 0.809339 | 1.99E-50 | -1.69E-46 |
| MS.gene050228 | MS.gene068950 | 0.802417 | 5.67E-49 | -1.69E-46 |
| MS.gene050330 | MS.gene068950 | -0.801321 | 9.52E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene068950 | 0.825116 | 5.59E-54 | -1.69E-46 |
| MS.gene050558 | MS.gene068950 | 0.816194 | 6.27E-52 | -1.69E-46 |
| MS.gene050560 | MS.gene068950 | 0.824793 | 6.66E-54 | -1.69E-46 |
| MS.gene050680 | MS.gene068950 | 0.8302 | 3.36E-55 | -1.69E-46 |
| MS.gene050787 | MS.gene068950 | 0.831789 | 1.37E-55 | -1.69E-46 |
| MS.gene050820 | MS.gene068950 | 0.816493 | 5.38E-52 | -1.69E-46 |
| MS.gene050826 | MS.gene068950 | 0.802892 | 4.52E-49 | -1.69E-46 |
| MS.gene05119 | MS.gene068950 | -0.814824 | 1.27E-51 | -1.69E-46 |
| MS.gene051265 | MS.gene068950 | 0.829783 | 4.24E-55 | -1.69E-46 |
| MS.gene051860 | MS.gene068950 | 0.809242 | 2.08E-50 | -1.69E-46 |
| MS.gene052190 | MS.gene068950 | 0.815453 | 9.17E-52 | -1.69E-46 |
| MS.gene053836 | MS.gene068950 | 0.807777 | 4.28E-50 | -1.69E-46 |
| MS.gene054467 | MS.gene068950 | 0.800887 | 1.17E-48 | -1.69E-46 |
| MS.gene054468 | MS.gene068950 | 0.810467 | 1.14E-50 | -1.69E-46 |
| MS.gene054473 | MS.gene068950 | 0.81522 | 1.03E-51 | -1.69E-46 |
| MS.gene054542 | MS.gene068950 | 0.804072 | 2.57E-49 | -1.69E-46 |
| MS.gene054559 | MS.gene068950 | 0.800452 | 1.43E-48 | -1.69E-46 |
| MS.gene054833 | MS.gene068950 | 0.809027 | 2.32E-50 | -1.69E-46 |
| MS.gene054930 | MS.gene068950 | 0.833561 | 4.97E-56 | -1.69E-46 |
| MS.gene055356 | MS.gene068950 | 0.832693 | 8.17E-56 | -1.69E-46 |
| MS.gene055357 | MS.gene068950 | 0.801415 | 9.10E-49 | -1.69E-46 |
| MS.gene055358 | MS.gene068950 | 0.850549 | 1.58E-60 | -1.69E-46 |
| MS.gene057194 | MS.gene068950 | 0.801143 | 1.03E-48 | -1.69E-46 |
| MS.gene057469 | MS.gene068950 | 0.855954 | 4.46E-62 | -1.69E-46 |
| MS.gene059128 | MS.gene068950 | 0.855984 | 4.37E-62 | -1.69E-46 |
| MS.gene060314 | MS.gene068950 | 0.806957 | 6.39E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene068950 | 0.826095 | 3.27E-54 | -1.69E-46 |
| MS.gene061114 | MS.gene068950 | -0.820421 | 6.92E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068950.t1 | MTR_8g056940 | 95.434 | 219 | 10 | 0 | 1 | 219 | 1 | 219 | 4.97e-155 | 428 |
| MS.gene068950.t1 | MTR_2g070060 | 65.297 | 219 | 76 | 0 | 1 | 219 | 1 | 219 | 4.29e-104 | 300 |
| MS.gene068950.t1 | MTR_2g070070 | 65.753 | 219 | 75 | 0 | 1 | 219 | 1 | 219 | 1.31e-103 | 298 |
| MS.gene068950.t1 | MTR_2g070180 | 64.486 | 214 | 76 | 0 | 1 | 214 | 1 | 214 | 2.68e-99 | 287 |
| MS.gene068950.t1 | MTR_4g124130 | 63.636 | 220 | 79 | 1 | 1 | 219 | 1 | 220 | 8.58e-99 | 286 |
| MS.gene068950.t1 | MTR_2g070150 | 66.832 | 202 | 67 | 0 | 1 | 202 | 1 | 202 | 7.03e-98 | 283 |
| MS.gene068950.t1 | MTR_0186s0030 | 97.872 | 141 | 3 | 0 | 1 | 141 | 1 | 141 | 2.56e-96 | 277 |
| MS.gene068950.t1 | MTR_2g070130 | 59.817 | 219 | 88 | 0 | 1 | 219 | 1 | 219 | 3.26e-96 | 280 |
| MS.gene068950.t1 | MTR_2g070140 | 65.025 | 203 | 71 | 0 | 17 | 219 | 1 | 203 | 4.80e-96 | 278 |
| MS.gene068950.t1 | MTR_2g070110 | 62.100 | 219 | 82 | 1 | 1 | 219 | 1 | 218 | 1.09e-94 | 276 |
| MS.gene068950.t1 | MTR_2g070200 | 60.455 | 220 | 85 | 2 | 1 | 218 | 1 | 220 | 2.26e-92 | 270 |
| MS.gene068950.t1 | MTR_2g070210 | 59.722 | 216 | 85 | 1 | 1 | 214 | 1 | 216 | 6.78e-91 | 266 |
| MS.gene068950.t1 | MTR_3g467420 | 54.884 | 215 | 97 | 0 | 5 | 219 | 10 | 224 | 2.61e-90 | 265 |
| MS.gene068950.t1 | MTR_3g467430 | 54.378 | 217 | 99 | 0 | 3 | 219 | 8 | 224 | 5.10e-89 | 261 |
| MS.gene068950.t1 | MTR_2g070120 | 56.808 | 213 | 92 | 0 | 1 | 213 | 1 | 213 | 1.06e-83 | 248 |
| MS.gene068950.t1 | MTR_5g076900 | 52.336 | 214 | 100 | 2 | 2 | 214 | 4 | 216 | 4.35e-73 | 221 |
| MS.gene068950.t1 | MTR_7g065720 | 40.000 | 220 | 125 | 5 | 3 | 217 | 6 | 223 | 2.23e-45 | 150 |
| MS.gene068950.t1 | MTR_7g065750 | 40.639 | 219 | 121 | 5 | 3 | 217 | 6 | 219 | 2.29e-45 | 150 |
| MS.gene068950.t1 | MTR_7g065740 | 41.096 | 219 | 120 | 5 | 3 | 217 | 6 | 219 | 1.38e-44 | 149 |
| MS.gene068950.t1 | MTR_4g019780 | 38.756 | 209 | 123 | 4 | 3 | 208 | 7 | 213 | 2.00e-44 | 148 |
| MS.gene068950.t1 | MTR_3g099757 | 47.159 | 176 | 91 | 2 | 1 | 175 | 1 | 175 | 8.86e-44 | 146 |
| MS.gene068950.t1 | MTR_7g065660 | 40.191 | 209 | 120 | 4 | 3 | 208 | 6 | 212 | 9.27e-44 | 146 |
| MS.gene068950.t1 | MTR_8g087425 | 36.406 | 217 | 135 | 2 | 2 | 216 | 3 | 218 | 1.40e-43 | 146 |
| MS.gene068950.t1 | MTR_4g059730 | 40.376 | 213 | 122 | 3 | 1 | 209 | 1 | 212 | 4.53e-43 | 144 |
| MS.gene068950.t1 | MTR_7g065630 | 45.699 | 186 | 96 | 5 | 3 | 187 | 24 | 205 | 2.85e-42 | 143 |
| MS.gene068950.t1 | MTR_1g090060 | 39.901 | 203 | 117 | 3 | 11 | 209 | 10 | 211 | 4.10e-42 | 142 |
| MS.gene068950.t1 | MTR_5g040430 | 35.610 | 205 | 125 | 2 | 2 | 199 | 5 | 209 | 1.68e-41 | 140 |
| MS.gene068950.t1 | MTR_4g019790 | 38.942 | 208 | 122 | 4 | 4 | 208 | 7 | 212 | 1.76e-41 | 140 |
| MS.gene068950.t1 | MTR_5g037380 | 43.689 | 206 | 101 | 7 | 2 | 195 | 3 | 205 | 3.84e-41 | 140 |
| MS.gene068950.t1 | MTR_7g065265 | 40.191 | 209 | 115 | 6 | 13 | 217 | 16 | 218 | 6.19e-40 | 137 |
| MS.gene068950.t1 | MTR_7g065710 | 48.428 | 159 | 79 | 3 | 1 | 158 | 1 | 157 | 7.97e-40 | 136 |
| MS.gene068950.t1 | MTR_7g065270 | 38.942 | 208 | 119 | 5 | 13 | 217 | 16 | 218 | 9.94e-40 | 136 |
| MS.gene068950.t1 | MTR_8g087410 | 40.884 | 181 | 104 | 2 | 7 | 185 | 9 | 188 | 1.28e-39 | 135 |
| MS.gene068950.t1 | MTR_7g065230 | 39.904 | 208 | 112 | 7 | 13 | 217 | 16 | 213 | 7.57e-38 | 131 |
| MS.gene068950.t1 | MTR_6g080440 | 38.835 | 206 | 116 | 5 | 18 | 216 | 17 | 219 | 1.04e-37 | 131 |
| MS.gene068950.t1 | MTR_7g065590 | 38.806 | 201 | 116 | 5 | 3 | 199 | 6 | 203 | 1.10e-37 | 130 |
| MS.gene068950.t1 | MTR_8g061950 | 40.909 | 198 | 108 | 5 | 7 | 195 | 8 | 205 | 1.98e-37 | 130 |
| MS.gene068950.t1 | MTR_1g090100 | 40.594 | 202 | 115 | 3 | 11 | 208 | 9 | 209 | 2.66e-37 | 130 |
| MS.gene068950.t1 | MTR_1g115195 | 39.109 | 202 | 113 | 4 | 7 | 201 | 6 | 204 | 4.27e-37 | 129 |
| MS.gene068950.t1 | MTR_7g065290 | 37.500 | 208 | 122 | 5 | 13 | 217 | 16 | 218 | 5.61e-37 | 129 |
| MS.gene068950.t1 | MTR_7g065680 | 47.771 | 157 | 79 | 3 | 3 | 158 | 6 | 160 | 8.14e-37 | 128 |
| MS.gene068950.t1 | MTR_1g090150 | 36.967 | 211 | 125 | 5 | 11 | 216 | 10 | 217 | 8.80e-37 | 129 |
| MS.gene068950.t1 | MTR_1g090070 | 38.140 | 215 | 119 | 5 | 4 | 209 | 9 | 218 | 1.75e-36 | 128 |
| MS.gene068950.t1 | MTR_7g065600 | 41.799 | 189 | 103 | 5 | 3 | 187 | 6 | 191 | 5.18e-36 | 126 |
| MS.gene068950.t1 | MTR_1g090090 | 39.604 | 202 | 117 | 4 | 11 | 208 | 10 | 210 | 4.64e-35 | 124 |
| MS.gene068950.t1 | MTR_7g065640 | 46.377 | 138 | 72 | 2 | 3 | 139 | 6 | 142 | 6.56e-35 | 123 |
| MS.gene068950.t1 | MTR_7g065700 | 48.387 | 155 | 77 | 3 | 3 | 156 | 6 | 158 | 7.01e-35 | 124 |
| MS.gene068950.t1 | MTR_7g065260 | 38.298 | 188 | 111 | 4 | 3 | 187 | 13 | 198 | 4.28e-33 | 119 |
| MS.gene068950.t1 | MTR_2g072120 | 63.889 | 72 | 26 | 0 | 47 | 118 | 1 | 72 | 1.08e-27 | 100 |
| MS.gene068950.t1 | MTR_4g043640 | 69.355 | 62 | 13 | 2 | 131 | 190 | 29 | 86 | 8.50e-23 | 88.6 |
| MS.gene068950.t1 | MTR_7g100320 | 28.169 | 213 | 135 | 8 | 10 | 216 | 32 | 232 | 1.02e-11 | 62.8 |
| MS.gene068950.t1 | MTR_5g037390 | 45.205 | 73 | 38 | 2 | 28 | 98 | 11 | 83 | 3.24e-11 | 58.5 |
| MS.gene068950.t1 | MTR_4g134380 | 28.655 | 171 | 105 | 5 | 3 | 159 | 14 | 181 | 5.44e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068950.t1 | AT1G78380 | 64.815 | 216 | 76 | 0 | 1 | 216 | 1 | 216 | 7.43e-101 | 291 |
| MS.gene068950.t1 | AT1G17170 | 62.100 | 219 | 82 | 1 | 1 | 219 | 1 | 218 | 2.29e-97 | 283 |
| MS.gene068950.t1 | AT1G78370 | 60.930 | 215 | 84 | 0 | 1 | 215 | 1 | 215 | 2.52e-97 | 282 |
| MS.gene068950.t1 | AT1G17180 | 60.274 | 219 | 87 | 0 | 1 | 219 | 1 | 219 | 1.57e-95 | 278 |
| MS.gene068950.t1 | AT1G78340 | 61.682 | 214 | 82 | 0 | 1 | 214 | 1 | 214 | 1.89e-94 | 275 |
| MS.gene068950.t1 | AT1G78360 | 60.000 | 220 | 87 | 1 | 1 | 219 | 1 | 220 | 1.47e-93 | 273 |
| MS.gene068950.t1 | AT1G78320 | 60.280 | 214 | 85 | 0 | 1 | 214 | 1 | 214 | 2.71e-89 | 262 |
| MS.gene068950.t1 | AT1G17190 | 55.607 | 214 | 95 | 0 | 3 | 216 | 4 | 217 | 2.78e-85 | 252 |
| MS.gene068950.t1 | AT1G53680 | 52.995 | 217 | 100 | 2 | 5 | 219 | 8 | 224 | 8.84e-83 | 246 |
| MS.gene068950.t1 | AT3G43800 | 53.302 | 212 | 95 | 3 | 3 | 210 | 4 | 215 | 3.06e-72 | 219 |
| MS.gene068950.t1 | AT1G78320 | 51.402 | 214 | 77 | 1 | 1 | 214 | 1 | 187 | 6.68e-69 | 209 |
| MS.gene068950.t1 | AT2G29420 | 40.654 | 214 | 116 | 5 | 2 | 209 | 7 | 215 | 9.11e-48 | 157 |
| MS.gene068950.t1 | AT3G09270 | 47.340 | 188 | 96 | 2 | 3 | 187 | 5 | 192 | 2.52e-47 | 155 |
| MS.gene068950.t1 | AT1G10370 | 38.889 | 216 | 124 | 4 | 2 | 209 | 3 | 218 | 4.72e-44 | 147 |
| MS.gene068950.t1 | AT2G29460 | 42.233 | 206 | 113 | 4 | 3 | 204 | 6 | 209 | 1.29e-43 | 146 |
| MS.gene068950.t1 | AT1G74590 | 35.000 | 220 | 131 | 4 | 5 | 215 | 8 | 224 | 1.11e-42 | 144 |
| MS.gene068950.t1 | AT2G29480 | 46.584 | 161 | 82 | 2 | 3 | 160 | 6 | 165 | 1.85e-42 | 143 |
| MS.gene068950.t1 | AT1G10360 | 42.051 | 195 | 105 | 4 | 2 | 188 | 3 | 197 | 5.27e-42 | 142 |
| MS.gene068950.t1 | AT2G29490 | 41.000 | 200 | 114 | 2 | 3 | 199 | 6 | 204 | 1.11e-41 | 141 |
| MS.gene068950.t1 | AT1G59700 | 40.359 | 223 | 122 | 6 | 3 | 215 | 5 | 226 | 1.90e-41 | 141 |
| MS.gene068950.t1 | AT2G29450 | 49.693 | 163 | 75 | 4 | 3 | 160 | 5 | 165 | 3.10e-41 | 140 |
| MS.gene068950.t1 | AT2G29440 | 48.485 | 165 | 73 | 4 | 3 | 160 | 5 | 164 | 3.15e-41 | 140 |
| MS.gene068950.t1 | AT2G29470 | 40.500 | 200 | 112 | 5 | 7 | 201 | 10 | 207 | 3.26e-41 | 140 |
| MS.gene068950.t1 | AT1G59670 | 40.090 | 222 | 124 | 5 | 3 | 215 | 5 | 226 | 1.07e-39 | 136 |
| MS.gene068950.t1 | AT1G27130 | 36.652 | 221 | 128 | 5 | 7 | 218 | 9 | 226 | 1.27e-39 | 136 |
| MS.gene068950.t1 | AT5G62480 | 37.989 | 179 | 103 | 1 | 5 | 175 | 9 | 187 | 3.09e-38 | 133 |
| MS.gene068950.t1 | AT1G27140 | 39.512 | 205 | 114 | 4 | 15 | 209 | 17 | 221 | 4.87e-38 | 132 |
| MS.gene068950.t1 | AT1G69930 | 35.885 | 209 | 127 | 3 | 7 | 208 | 15 | 223 | 1.92e-36 | 128 |
| MS.gene068950.t1 | AT1G69920 | 35.426 | 223 | 128 | 6 | 7 | 218 | 37 | 254 | 1.09e-34 | 124 |
| MS.gene068950.t1 | AT5G62480 | 29.050 | 179 | 92 | 2 | 5 | 175 | 9 | 160 | 1.05e-20 | 86.7 |
| MS.gene068950.t1 | AT5G62480 | 29.050 | 179 | 93 | 2 | 5 | 175 | 9 | 161 | 1.61e-20 | 86.3 |
Find 49 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAAGCTCAAGCCAGATTT+TGG | 0.160142 | 8.1:-58207465 | MS.gene068950:CDS |
| AGGATAGCATTGGCTGAAAA+AGG | 0.234872 | 8.1:-58207679 | MS.gene068950:CDS |
| ATAATTATGGACTCAGAAAT+TGG | 0.235033 | 8.1:+58207550 | None:intergenic |
| CTTGGAGACCAACCTTATTT+TGG | 0.247910 | 8.1:-58207023 | MS.gene068950:CDS |
| GGCAGAAGAGTTGATATTAT+TGG | 0.258477 | 8.1:-58207734 | MS.gene068950:CDS |
| GGAGTTGATTGAAGGCTTAA+AGG | 0.261757 | 8.1:-58207060 | MS.gene068950:CDS |
| AATATACTCAACAATAATTA+TGG | 0.276052 | 8.1:+58207537 | None:intergenic |
| TTTCTCATCCGCAACAGATT+TGG | 0.278908 | 8.1:+58206851 | None:intergenic |
| CTGGAATCTTCTTGTGAATT+GGG | 0.294128 | 8.1:+58207593 | None:intergenic |
| ACTGGAATCTTCTTGTGAAT+TGG | 0.295626 | 8.1:+58207592 | None:intergenic |
| CTACAGGAAGAGGTTTGATT+TGG | 0.324310 | 8.1:-58206805 | MS.gene068950:CDS |
| GTGATGTTGGAGGAAGGATA+TGG | 0.346197 | 8.1:-58207118 | MS.gene068950:CDS |
| GACAAAATCAGCCCAAAATC+TGG | 0.350630 | 8.1:+58207453 | None:intergenic |
| GGTTCATTTGCAAAAGCATT+TGG | 0.357554 | 8.1:+58207614 | None:intergenic |
| TAAGGATCTGAGGGTAGAAA+TGG | 0.386916 | 8.1:+58207490 | None:intergenic |
| CTGGCTTGAGCTTTCTCATA+AGG | 0.391510 | 8.1:+58207472 | None:intergenic |
| GATGAATGGCTTAGTATGTT+TGG | 0.396742 | 8.1:-58207712 | MS.gene068950:CDS |
| TTTCCCTTATGGATAAGAAC+TGG | 0.413273 | 8.1:+58207574 | None:intergenic |
| AGTTGATATTATTGGATGAA+TGG | 0.416995 | 8.1:-58207726 | MS.gene068950:CDS |
| AAAGAGAGATGAGATTGAAT+TGG | 0.418834 | 8.1:-58207090 | MS.gene068950:CDS |
| GATTCCAGTTCTTATCCATA+AGG | 0.427078 | 8.1:-58207578 | MS.gene068950:CDS |
| TTGAGTATATTGATGAGGTT+TGG | 0.428745 | 8.1:-58207525 | MS.gene068950:CDS |
| AATGTGTCACCACCAAAATA+AGG | 0.439985 | 8.1:+58207011 | None:intergenic |
| TGAGATTGAATTGGCAAAGA+AGG | 0.443100 | 8.1:-58207081 | MS.gene068950:CDS |
| TGATGAGGTTTGGAAGGATA+AGG | 0.450626 | 8.1:-58207515 | MS.gene068950:CDS |
| TATAAAACCAACTATGAAAA+GGG | 0.461833 | 8.1:+58206964 | None:intergenic |
| ATTCCAGTTCTTATCCATAA+GGG | 0.462545 | 8.1:-58207577 | MS.gene068950:CDS |
| GTATAAAACCAACTATGAAA+AGG | 0.463905 | 8.1:+58206963 | None:intergenic |
| AAAATGCAGGTTGGTGATGT+TGG | 0.476506 | 8.1:-58207131 | MS.gene068950:intron |
| GCAAAGAAGGAGTTGATTGA+AGG | 0.480756 | 8.1:-58207068 | MS.gene068950:CDS |
| TGATGTTGGAGGAAGGATAT+GGG | 0.485671 | 8.1:-58207117 | MS.gene068950:CDS |
| AAGGAATTAGAGAATGTGCT+TGG | 0.497037 | 8.1:-58207041 | MS.gene068950:CDS |
| AGGGATAAAGTATGAGTACA+AGG | 0.524364 | 8.1:-58207659 | MS.gene068950:CDS |
| GTGTGATAAGTTGATTGCTT+GGG | 0.528271 | 8.1:-58206904 | MS.gene068950:CDS |
| AGCTTTCTCATAAGGATCTG+AGG | 0.541397 | 8.1:+58207480 | None:intergenic |
| ATTATGCAACTTCAAAGTTG+AGG | 0.546513 | 8.1:-58206931 | MS.gene068950:CDS |
| GTATATTGATGAGGTTTGGA+AGG | 0.548215 | 8.1:-58207521 | MS.gene068950:CDS |
| GTTGGAGGAAGGATATGGGC+TGG | 0.551186 | 8.1:-58207113 | MS.gene068950:CDS |
| TTGTTGTTGACTACAGGAAG+AGG | 0.552913 | 8.1:-58206815 | MS.gene068950:CDS |
| AAGGATCTGAGGGTAGAAAT+GGG | 0.556042 | 8.1:+58207491 | None:intergenic |
| GTATGTTTGGCATGAGAGCA+AGG | 0.570950 | 8.1:-58207699 | MS.gene068950:CDS |
| CATGAGAGCAAGGATAGCAT+TGG | 0.573949 | 8.1:-58207689 | MS.gene068950:CDS |
| AGTGTGATAAGTTGATTGCT+TGG | 0.574483 | 8.1:-58206905 | MS.gene068950:CDS |
| GCTTTCTCATAAGGATCTGA+GGG | 0.584199 | 8.1:+58207481 | None:intergenic |
| GAGTGTTTCCAAATCTGTTG+CGG | 0.586353 | 8.1:-58206859 | MS.gene068950:CDS |
| TATTGTTGAGTATATTGATG+AGG | 0.599628 | 8.1:-58207530 | MS.gene068950:CDS |
| ATGCAGGTTGGTGATGTTGG+AGG | 0.604533 | 8.1:-58207128 | MS.gene068950:intron |
| TGTCACCACCAAAATAAGGT+TGG | 0.621870 | 8.1:+58207015 | None:intergenic |
| AGGTTGGTGATGTTGGAGGA+AGG | 0.655636 | 8.1:-58207124 | MS.gene068950:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGATATTTTTATTTATTTAC+TGG | + | chr8.1:58207182-58207201 | None:intergenic | 10.0% |
| !! | AATATACTCAACAATAATTA+TGG | + | chr8.1:58207002-58207021 | None:intergenic | 15.0% |
| !!! | ATTTTTATTTATTTACTGGT+AGG | + | chr8.1:58207178-58207197 | None:intergenic | 15.0% |
| !! | AATGTGTTAAAAAAAAATGC+AGG | - | chr8.1:58207392-58207411 | MS.gene068950:intron | 20.0% |
| !! | TATAAAACCAACTATGAAAA+GGG | + | chr8.1:58207575-58207594 | None:intergenic | 20.0% |
| ! | AAATATGTTAAGCCATTGAA+TGG | + | chr8.1:58207152-58207171 | None:intergenic | 25.0% |
| ! | AATATGTTAAGCCATTGAAT+GGG | + | chr8.1:58207151-58207170 | None:intergenic | 25.0% |
| ! | ATAATTATGGACTCAGAAAT+TGG | + | chr8.1:58206989-58207008 | None:intergenic | 25.0% |
| ! | CAACCAAGTCTTTAATAATA+TGG | + | chr8.1:58207332-58207351 | None:intergenic | 25.0% |
| ! | GATCCATATTATTAAAGACT+TGG | - | chr8.1:58207326-58207345 | MS.gene068950:intron | 25.0% |
| ! | GTATAAAACCAACTATGAAA+AGG | + | chr8.1:58207576-58207595 | None:intergenic | 25.0% |
| ! | TATTGTTGAGTATATTGATG+AGG | - | chr8.1:58207006-58207025 | MS.gene068950:CDS | 25.0% |
| ! | TGTTAAAAAAAAATGCAGGT+TGG | - | chr8.1:58207396-58207415 | MS.gene068950:intron | 25.0% |
| !! | AGTTGATATTATTGGATGAA+TGG | - | chr8.1:58206810-58206829 | MS.gene068950:CDS | 25.0% |
| AAAGAGAGATGAGATTGAAT+TGG | - | chr8.1:58207446-58207465 | MS.gene068950:CDS | 30.0% | |
| ATTATGCAACTTCAAAGTTG+AGG | - | chr8.1:58207605-58207624 | MS.gene068950:CDS | 30.0% | |
| ATTCCAGTTCTTATCCATAA+GGG | - | chr8.1:58206959-58206978 | MS.gene068950:CDS | 30.0% | |
| GTATAGGAAACATTCATCAA+TGG | - | chr8.1:58207278-58207297 | MS.gene068950:intron | 30.0% | |
| TTGAGTATATTGATGAGGTT+TGG | - | chr8.1:58207011-58207030 | MS.gene068950:CDS | 30.0% | |
| !!! | ACAAGAAGGTAAGTTTTGTT+AGG | - | chr8.1:58207107-58207126 | MS.gene068950:CDS | 30.0% |
| !!! | ATGATTTTGTTGTTGACTAC+AGG | - | chr8.1:58207715-58207734 | MS.gene068950:CDS | 30.0% |
| AAGGAATTAGAGAATGTGCT+TGG | - | chr8.1:58207495-58207514 | MS.gene068950:CDS | 35.0% | |
| AATGTGTCACCACCAAAATA+AGG | + | chr8.1:58207528-58207547 | None:intergenic | 35.0% | |
| ACTGGAATCTTCTTGTGAAT+TGG | + | chr8.1:58206947-58206966 | None:intergenic | 35.0% | |
| AGGAAACATTCATCAATGGA+TGG | - | chr8.1:58207282-58207301 | MS.gene068950:intron | 35.0% | |
| AGGGATAAAGTATGAGTACA+AGG | - | chr8.1:58206877-58206896 | MS.gene068950:CDS | 35.0% | |
| ATACTCATATCTCACTAGGA+AGG | + | chr8.1:58207262-58207281 | None:intergenic | 35.0% | |
| CTGGAATCTTCTTGTGAATT+GGG | + | chr8.1:58206946-58206965 | None:intergenic | 35.0% | |
| GATGAATGGCTTAGTATGTT+TGG | - | chr8.1:58206824-58206843 | MS.gene068950:CDS | 35.0% | |
| GATTCCAGTTCTTATCCATA+AGG | - | chr8.1:58206958-58206977 | MS.gene068950:CDS | 35.0% | |
| GTATATTGATGAGGTTTGGA+AGG | - | chr8.1:58207015-58207034 | MS.gene068950:CDS | 35.0% | |
| TCCTAGTGAGATATGAGTAT+AGG | - | chr8.1:58207262-58207281 | MS.gene068950:intron | 35.0% | |
| TCCTATACTCATATCTCACT+AGG | + | chr8.1:58207266-58207285 | None:intergenic | 35.0% | |
| TGAGATTGAATTGGCAAAGA+AGG | - | chr8.1:58207455-58207474 | MS.gene068950:CDS | 35.0% | |
| TTTCCCTTATGGATAAGAAC+TGG | + | chr8.1:58206965-58206984 | None:intergenic | 35.0% | |
| ! | CTTTGATCCCTTTTCATAGT+TGG | - | chr8.1:58207565-58207584 | MS.gene068950:CDS | 35.0% |
| !! | AGTGTGATAAGTTGATTGCT+TGG | - | chr8.1:58207631-58207650 | MS.gene068950:CDS | 35.0% |
| !! | GGCAGAAGAGTTGATATTAT+TGG | - | chr8.1:58206802-58206821 | MS.gene068950:CDS | 35.0% |
| !! | GGTTCATTTGCAAAAGCATT+TGG | + | chr8.1:58206925-58206944 | None:intergenic | 35.0% |
| !! | GTGTGATAAGTTGATTGCTT+GGG | - | chr8.1:58207632-58207651 | MS.gene068950:CDS | 35.0% |
| !!! | ATTTTGGTGGTGACACATTT+GGG | - | chr8.1:58207529-58207548 | MS.gene068950:CDS | 35.0% |
| !!! | CAGAAATTGGTTTTCCCTTA+TGG | + | chr8.1:58206976-58206995 | None:intergenic | 35.0% |
| !!! | TATTTTGGTGGTGACACATT+TGG | - | chr8.1:58207528-58207547 | MS.gene068950:CDS | 35.0% |
| AAGGATCTGAGGGTAGAAAT+GGG | + | chr8.1:58207048-58207067 | None:intergenic | 40.0% | |
| AGCTTTCTCATAAGGATCTG+AGG | + | chr8.1:58207059-58207078 | None:intergenic | 40.0% | |
| CATATCTCACTAGGAAGGAA+AGG | + | chr8.1:58207257-58207276 | None:intergenic | 40.0% | |
| CTACAGGAAGAGGTTTGATT+TGG | - | chr8.1:58207731-58207750 | MS.gene068950:CDS | 40.0% | |
| GAATGGGACACAAAGAACTT+TGG | + | chr8.1:58207135-58207154 | None:intergenic | 40.0% | |
| GACAAAATCAGCCCAAAATC+TGG | + | chr8.1:58207086-58207105 | None:intergenic | 40.0% | |
| GAGTGTTTCCAAATCTGTTG+CGG | - | chr8.1:58207677-58207696 | MS.gene068950:CDS | 40.0% | |
| GCTTTCTCATAAGGATCTGA+GGG | + | chr8.1:58207058-58207077 | None:intergenic | 40.0% | |
| GTTCTTTGTGTCCCATTCAA+TGG | - | chr8.1:58207137-58207156 | MS.gene068950:intron | 40.0% | |
| TAAGGATCTGAGGGTAGAAA+TGG | + | chr8.1:58207049-58207068 | None:intergenic | 40.0% | |
| TGATGAGGTTTGGAAGGATA+AGG | - | chr8.1:58207021-58207040 | MS.gene068950:CDS | 40.0% | |
| TGATGTTGGAGGAAGGATAT+GGG | - | chr8.1:58207419-58207438 | MS.gene068950:intron | 40.0% | |
| TGTCACCACCAAAATAAGGT+TGG | + | chr8.1:58207524-58207543 | None:intergenic | 40.0% | |
| TTTCTCATCCGCAACAGATT+TGG | + | chr8.1:58207688-58207707 | None:intergenic | 40.0% | |
| ! | AGAAAGCTCAAGCCAGATTT+TGG | - | chr8.1:58207071-58207090 | MS.gene068950:CDS | 40.0% |
| ! | GAAAGCTCAAGCCAGATTTT+GGG | - | chr8.1:58207072-58207091 | MS.gene068950:CDS | 40.0% |
| ! | GCAAAGAAGGAGTTGATTGA+AGG | - | chr8.1:58207468-58207487 | MS.gene068950:CDS | 40.0% |
| ! | GGAGTTGATTGAAGGCTTAA+AGG | - | chr8.1:58207476-58207495 | MS.gene068950:CDS | 40.0% |
| ! | TTGTTGTTGACTACAGGAAG+AGG | - | chr8.1:58207721-58207740 | MS.gene068950:CDS | 40.0% |
| !! | AAAATGCAGGTTGGTGATGT+TGG | - | chr8.1:58207405-58207424 | MS.gene068950:intron | 40.0% |
| !! | AGGATAGCATTGGCTGAAAA+AGG | - | chr8.1:58206857-58206876 | MS.gene068950:CDS | 40.0% |
| !! | CTTGGAGACCAACCTTATTT+TGG | - | chr8.1:58207513-58207532 | MS.gene068950:CDS | 40.0% |
| !! | GGATAGCATTGGCTGAAAAA+GGG | - | chr8.1:58206858-58206877 | MS.gene068950:CDS | 40.0% |
| !! | GGCTGATTTTGTCAACAAGA+AGG | - | chr8.1:58207093-58207112 | MS.gene068950:CDS | 40.0% |
| CATGAGAGCAAGGATAGCAT+TGG | - | chr8.1:58206847-58206866 | MS.gene068950:CDS | 45.0% | |
| GTATGTTTGGCATGAGAGCA+AGG | - | chr8.1:58206837-58206856 | MS.gene068950:CDS | 45.0% | |
| GTGATGTTGGAGGAAGGATA+TGG | - | chr8.1:58207418-58207437 | MS.gene068950:intron | 45.0% | |
| ! | CTGGCTTGAGCTTTCTCATA+AGG | + | chr8.1:58207067-58207086 | None:intergenic | 45.0% |
| !!! | GGAGACCAACCTTATTTTGG+TGG | - | chr8.1:58207516-58207535 | MS.gene068950:CDS | 45.0% |
| !! | AGGTTGGTGATGTTGGAGGA+AGG | - | chr8.1:58207412-58207431 | MS.gene068950:intron | 50.0% |
| !! | ATGCAGGTTGGTGATGTTGG+AGG | - | chr8.1:58207408-58207427 | MS.gene068950:intron | 50.0% |
| GTTGGAGGAAGGATATGGGC+TGG | - | chr8.1:58207423-58207442 | MS.gene068950:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 58206800 | 58207758 | 58206800 | ID=MS.gene068950 |
| chr8.1 | mRNA | 58206800 | 58207758 | 58206800 | ID=MS.gene068950.t1;Parent=MS.gene068950 |
| chr8.1 | exon | 58207444 | 58207758 | 58207444 | ID=MS.gene068950.t1.exon1;Parent=MS.gene068950.t1 |
| chr8.1 | CDS | 58207444 | 58207758 | 58207444 | ID=cds.MS.gene068950.t1;Parent=MS.gene068950.t1 |
| chr8.1 | exon | 58206800 | 58207144 | 58206800 | ID=MS.gene068950.t1.exon2;Parent=MS.gene068950.t1 |
| chr8.1 | CDS | 58206800 | 58207144 | 58206800 | ID=cds.MS.gene068950.t1;Parent=MS.gene068950.t1 |
| Gene Sequence |
| Protein sequence |
