AlfalfaGEDB | MS.gene069966

M. sativa cultivar XinJiangDaYe / MS.gene069966

KEGG: K00888
GO: GO:0003674 GO:0003824 GO:0016301 GO:0016740 GO:0016772
PFAM: PF00454.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069966.t1	XP_003603887.2	94.4	107	6	0	2	108	1843	1949	2.60E-51	211.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069966.t1	Q9SXA1	85.0	107	16	0	2	108	1836	1942	2.7e-49	195.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069966.t1	G7J6H9	94.4	107	6	0	2	108	1843	1949	1.9e-51	211.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene069966	0.8285	8.68E-55	-1.69E-46
MS.gene049254	MS.gene069966	0.81228	4.59E-51	-1.69E-46
MS.gene049350	MS.gene069966	0.817359	3.44E-52	-1.69E-46
MS.gene049711	MS.gene069966	0.82111	4.80E-53	-1.69E-46
MS.gene049807	MS.gene069966	0.821971	3.04E-53	-1.69E-46
MS.gene049850	MS.gene069966	0.80506	1.60E-49	-1.69E-46
MS.gene050901	MS.gene069966	0.819342	1.22E-52	-1.69E-46
MS.gene051018	MS.gene069966	0.806781	6.96E-50	-1.69E-46
MS.gene051247	MS.gene069966	0.818186	2.23E-52	-1.69E-46
MS.gene051388	MS.gene069966	0.8138	2.13E-51	-1.69E-46
MS.gene051719	MS.gene069966	0.855277	7.03E-62	-1.69E-46
MS.gene051720	MS.gene069966	0.85467	1.05E-61	-1.69E-46
MS.gene051744	MS.gene069966	0.828924	6.86E-55	-1.69E-46
MS.gene051957	MS.gene069966	0.801267	9.76E-49	-1.69E-46
MS.gene052463	MS.gene069966	0.804605	1.99E-49	-1.69E-46
MS.gene052516	MS.gene069966	0.817148	3.83E-52	-1.69E-46
MS.gene052519	MS.gene069966	0.821781	3.36E-53	-1.69E-46
MS.gene053144	MS.gene069966	0.861968	7.07E-64	-1.69E-46
MS.gene053226	MS.gene069966	0.808299	3.32E-50	-1.69E-46
MS.gene053581	MS.gene069966	0.824368	8.38E-54	-1.69E-46
MS.gene053599	MS.gene069966	0.80294	4.42E-49	-1.69E-46
MS.gene05423	MS.gene069966	0.817858	2.65E-52	-1.69E-46
MS.gene054584	MS.gene069966	0.838829	2.28E-57	-1.69E-46
MS.gene054651	MS.gene069966	0.808447	3.08E-50	-1.69E-46
MS.gene054775	MS.gene069966	0.80965	1.70E-50	-1.69E-46
MS.gene054991	MS.gene069966	0.809007	2.34E-50	-1.69E-46
MS.gene055465	MS.gene069966	0.845038	5.21E-59	-1.69E-46
MS.gene055466	MS.gene069966	0.850546	1.58E-60	-1.69E-46
MS.gene055468	MS.gene069966	0.843389	1.44E-58	-1.69E-46
MS.gene055537	MS.gene069966	0.814273	1.68E-51	-1.69E-46
MS.gene055580	MS.gene069966	-0.819928	8.97E-53	-1.69E-46
MS.gene055818	MS.gene069966	0.808228	3.43E-50	-1.69E-46
MS.gene056050	MS.gene069966	0.802616	5.16E-49	-1.69E-46
MS.gene056308	MS.gene069966	0.81595	7.11E-52	-1.69E-46
MS.gene056595	MS.gene069966	0.827839	1.25E-54	-1.69E-46
MS.gene057294	MS.gene069966	0.803247	3.82E-49	-1.69E-46
MS.gene057444	MS.gene069966	0.80882	2.57E-50	-1.69E-46
MS.gene058625	MS.gene069966	0.827745	1.32E-54	-1.69E-46
MS.gene05894	MS.gene069966	0.808876	2.50E-50	-1.69E-46
MS.gene059015	MS.gene069966	0.837997	3.73E-57	-1.69E-46
MS.gene059084	MS.gene069966	0.805357	1.39E-49	-1.69E-46
MS.gene059625	MS.gene069966	0.826486	2.64E-54	-1.69E-46
MS.gene060519	MS.gene069966	0.822505	2.29E-53	-1.69E-46
MS.gene061100	MS.gene069966	0.805502	1.29E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069966.t1	MTR_3g116250	94.393	107	6	0	2	108	1843	1949	1.35e-65	214
MS.gene069966.t1	MTR_8g093570	55.128	78	34	1	17	94	912	988	1.57e-22	91.3
MS.gene069966.t1	MTR_3g096100	58.696	46	18	1	47	92	27	71	1.76e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069966.t1	AT1G49340	85.047	107	16	0	2	108	1829	1935	2.25e-60	199
MS.gene069966.t1	AT1G49340	85.047	107	16	0	2	108	1829	1935	2.25e-60	199
MS.gene069966.t1	AT1G49340	85.047	107	16	0	2	108	1836	1942	2.29e-60	199
MS.gene069966.t1	AT1G49340	85.047	107	16	0	2	108	1836	1942	2.29e-60	199
MS.gene069966.t1	AT1G51040	82.243	107	19	0	2	108	333	439	4.97e-59	189
MS.gene069966.t1	AT5G64070	56.410	78	33	1	17	94	944	1020	4.51e-23	92.8
MS.gene069966.t1	AT5G09350	56.410	78	33	1	17	94	939	1015	6.22e-23	92.4

Find 26 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCTTTACTTATCAAATAGA+AGG	0.334555	3.1:+91806052	None:intergenic
AAATATTTCAGCAAGGACTA+TGG	0.338107	3.1:-91806186	MS.gene069966:CDS
CACTGCTAATGATGAAGTTC+TGG	0.358922	3.1:+91806130	None:intergenic
TTCCCATTGTGCCGATCCTT+TGG	0.377218	3.1:+91806075	None:intergenic
CTGGCGTGCAGCCTCAAAAC+TGG	0.389867	3.1:+91806149	None:intergenic
GTTCCATGTATCACTCTTCA+TGG	0.400678	3.1:+91805660	None:intergenic
AACCAAAGGATCGGCACAAT+GGG	0.428176	3.1:-91806077	MS.gene069966:CDS
AACTTCATCATTAGCAGTGC+AGG	0.441078	3.1:-91806126	MS.gene069966:CDS
TTCATTCTAGAAACTTCTCC+AGG	0.464936	3.1:-91805757	MS.gene069966:CDS
AGGCTTGTTCATATTGACTT+CGG	0.529710	3.1:-91805781	MS.gene069966:CDS
TAGTCTCTTGCTTCAACCAA+AGG	0.542801	3.1:-91806091	MS.gene069966:CDS
AGCAAGGACTATGGACCTGT+CGG	0.548609	3.1:-91806177	MS.gene069966:CDS
ACACAATTACTAGATCCATC+TGG	0.559163	3.1:-91805685	MS.gene069966:CDS
TTCTAGAAACTTCTCCAGGT+GGG	0.561363	3.1:-91805753	MS.gene069966:CDS
CAACCAAAGGATCGGCACAA+TGG	0.570140	3.1:-91806078	MS.gene069966:CDS
GCAAGGACTATGGACCTGTC+GGG	0.579900	3.1:-91806176	MS.gene069966:CDS
TCACTCTTCATGGCACCAGA+TGG	0.580788	3.1:+91805670	None:intergenic
TATGCTGTTGCAGTGTTGGG+AGG	0.590277	3.1:-91805801	MS.gene069966:intron
CTAGTAATTGTGTCATCTCG+TGG	0.595750	3.1:+91805695	None:intergenic
TCAAAACTGGAAGACCCGAC+AGG	0.600790	3.1:+91806162	None:intergenic
ATTCTAGAAACTTCTCCAGG+TGG	0.613115	3.1:-91805754	MS.gene069966:CDS
TCTTGCTTCAACCAAAGGAT+CGG	0.613180	3.1:-91806086	MS.gene069966:CDS
TTGTTTGAAATATTTCAGCA+AGG	0.626092	3.1:-91806193	MS.gene069966:CDS
GTGCCATGAAGAGTGATACA+TGG	0.633071	3.1:-91805663	MS.gene069966:CDS
TCAAAACGCATATTCCCACC+TGG	0.645332	3.1:+91805739	None:intergenic
TGGGAGAAACAACTGATGGG+GGG	0.678437	3.1:-91806216	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATATTAGTCATAGATTTCT+TGG	+	chr3.1:91806026-91806045	None:intergenic	20.0%
!!	ATCTTTACTTATCAAATAGA+AGG	+	chr3.1:91805801-91805820	None:intergenic	20.0%
!!	TAAACTTAAATTAAATCCAC+TGG	+	chr3.1:91805869-91805888	None:intergenic	20.0%
!	ATCACAAATAAATTGAACCA+GGG	+	chr3.1:91805936-91805955	None:intergenic	25.0%
!!	TTGTTTGAAATATTTCAGCA+AGG	-	chr3.1:91805657-91805676	MS.gene069966:CDS	25.0%
	AAATATTTCAGCAAGGACTA+TGG	-	chr3.1:91805664-91805683	MS.gene069966:CDS	30.0%
	ATTCTTATTATAATCGCCAG+TGG	-	chr3.1:91805850-91805869	MS.gene069966:intron	30.0%
	GATCACAAATAAATTGAACC+AGG	+	chr3.1:91805937-91805956	None:intergenic	30.0%
	GGATCAATCAATTAGCATAT+AGG	+	chr3.1:91805914-91805933	None:intergenic	30.0%
	TCACAAATAAATTGAACCAG+GGG	+	chr3.1:91805935-91805954	None:intergenic	30.0%
	ACACAATTACTAGATCCATC+TGG	-	chr3.1:91806165-91806184	MS.gene069966:CDS	35.0%
	ATATATGCTGTTGCAGTGTT+GGG	-	chr3.1:91806046-91806065	MS.gene069966:intron	35.0%
	TATATATGCTGTTGCAGTGT+TGG	-	chr3.1:91806045-91806064	MS.gene069966:intron	35.0%
!	TTCATTCTAGAAACTTCTCC+AGG	-	chr3.1:91806093-91806112	MS.gene069966:CDS	35.0%
!!	AGGCTTGTTCATATTGACTT+CGG	-	chr3.1:91806069-91806088	MS.gene069966:CDS	35.0%
	AACTTCATCATTAGCAGTGC+AGG	-	chr3.1:91805724-91805743	MS.gene069966:CDS	40.0%
	AGTAGGAGAAGAAAACAGAG+AGG	+	chr3.1:91805974-91805993	None:intergenic	40.0%
	ATGCTAATTGATTGATCCCC+TGG	-	chr3.1:91805916-91805935	MS.gene069966:intron	40.0%
	CACTGCTAATGATGAAGTTC+TGG	+	chr3.1:91805723-91805742	None:intergenic	40.0%
	CTAGTAATTGTGTCATCTCG+TGG	+	chr3.1:91806158-91806177	None:intergenic	40.0%
	GTAGGAGAAGAAAACAGAGA+GGG	+	chr3.1:91805973-91805992	None:intergenic	40.0%
	GTTCCATGTATCACTCTTCA+TGG	+	chr3.1:91806193-91806212	None:intergenic	40.0%
	TAGGAGAAGAAAACAGAGAG+GGG	+	chr3.1:91805972-91805991	None:intergenic	40.0%
	TAGTCTCTTGCTTCAACCAA+AGG	-	chr3.1:91805759-91805778	MS.gene069966:CDS	40.0%
	TATACTTGCAGAGTTGCAGT+AGG	+	chr3.1:91805991-91806010	None:intergenic	40.0%
	TCTTGCTTCAACCAAAGGAT+CGG	-	chr3.1:91805764-91805783	MS.gene069966:CDS	40.0%
!	ATTCTAGAAACTTCTCCAGG+TGG	-	chr3.1:91806096-91806115	MS.gene069966:CDS	40.0%
!	TTCTAGAAACTTCTCCAGGT+GGG	-	chr3.1:91806097-91806116	MS.gene069966:CDS	40.0%
	AACCAAAGGATCGGCACAAT+GGG	-	chr3.1:91805773-91805792	MS.gene069966:CDS	45.0%
	GTGCCATGAAGAGTGATACA+TGG	-	chr3.1:91806187-91806206	MS.gene069966:CDS	45.0%
	TCAAAACGCATATTCCCACC+TGG	+	chr3.1:91806114-91806133	None:intergenic	45.0%
	AGCAAGGACTATGGACCTGT+CGG	-	chr3.1:91805673-91805692	MS.gene069966:CDS	50.0%
	CAACCAAAGGATCGGCACAA+TGG	-	chr3.1:91805772-91805791	MS.gene069966:CDS	50.0%
	TCAAAACTGGAAGACCCGAC+AGG	+	chr3.1:91805691-91805710	None:intergenic	50.0%
	TCACTCTTCATGGCACCAGA+TGG	+	chr3.1:91806183-91806202	None:intergenic	50.0%
!	TATGCTGTTGCAGTGTTGGG+AGG	-	chr3.1:91806049-91806068	MS.gene069966:intron	50.0%
!	TGTCGGGTCTTCCAGTTTTG+AGG	-	chr3.1:91805690-91805709	MS.gene069966:CDS	50.0%
!	TTCCCATTGTGCCGATCCTT+TGG	+	chr3.1:91805778-91805797	None:intergenic	50.0%
	GCAAGGACTATGGACCTGTC+GGG	-	chr3.1:91805674-91805693	MS.gene069966:CDS	55.0%
	CTGGCGTGCAGCCTCAAAAC+TGG	+	chr3.1:91805704-91805723	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	91805649	91806223	91805649	ID=MS.gene069966
chr3.1	mRNA	91805649	91806223	91805649	ID=MS.gene069966.t1;Parent=MS.gene069966
chr3.1	exon	91806060	91806223	91806060	ID=MS.gene069966.t1.exon1;Parent=MS.gene069966.t1
chr3.1	CDS	91806060	91806223	91806060	ID=cds.MS.gene069966.t1;Parent=MS.gene069966.t1
chr3.1	exon	91805649	91805810	91805649	ID=MS.gene069966.t1.exon2;Parent=MS.gene069966.t1
chr3.1	CDS	91805649	91805810	91805649	ID=cds.MS.gene069966.t1;Parent=MS.gene069966.t1

Gene Sequence

>MS.gene069966

ATGGGGGGTTGTTTGAAATATTTCAGCAAGGACTATGGACCTGTCGGGTCTTCCAGTTTTGAGGCTGCACGCCAGAACTTCATCATTAGCAGTGCAGGTTATGCTGTTGCTAGTCTCTTGCTTCAACCAAAGGATCGGCACAATGGGAACCTTCTATTTGATAAGTAAAGATATTTATATTTCTTCATTAATTTGTCAGCAATTCTTATTATAATCGCCAGTGGATTTAATTTAAGTTTAGCTTTATATGTATCTGAAACATCCTATATGCTAATTGATTGATCCCCTGGTTCAATTTATTTGTGATCTTCAGTTCTCTTCCCCTCTCTGTTTTCTTCTCCTACTGCAACTCTGCAAGTATATGAAGTACTGATCCAAGAAATCTATGACTAATATTATATATGCTGTTGCAGTGTTGGGAGGCTTGTTCATATTGACTTCGGTTTCATTCTAGAAACTTCTCCAGGTGGGAATATGCGTTTTGAAAGTGCACATTTTAAGCTGAGCCACGAGATGACACAATTACTAGATCCATCTGGTGCCATGAAGAGTGATACATGGAACCAATTTTTAAG

Protein sequence

>MS.gene069966.t1

MGGCLKYFSKDYGPVGSSSFEAARQNFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGAMKSDTWNQFL