Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070564.t1 | XP_003613754.1 | 91.6 | 225 | 19 | 0 | 1 | 225 | 1 | 225 | 3.70E-116 | 427.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070564.t1 | Q9CA57 | 43.2 | 220 | 124 | 1 | 1 | 220 | 1 | 219 | 3.2e-52 | 206.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070564.t1 | G7KDY4 | 91.6 | 225 | 19 | 0 | 1 | 225 | 1 | 225 | 2.7e-116 | 427.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene070564 | 0.801917 | 7.18E-49 | -1.69E-46 |
| MS.gene05240 | MS.gene070564 | 0.806846 | 6.75E-50 | -1.69E-46 |
| MS.gene05244 | MS.gene070564 | 0.802954 | 4.39E-49 | -1.69E-46 |
| MS.gene052702 | MS.gene070564 | 0.809142 | 2.19E-50 | -1.69E-46 |
| MS.gene053420 | MS.gene070564 | 0.800418 | 1.45E-48 | -1.69E-46 |
| MS.gene054181 | MS.gene070564 | 0.801818 | 7.52E-49 | -1.69E-46 |
| MS.gene056359 | MS.gene070564 | 0.80131 | 9.56E-49 | -1.69E-46 |
| MS.gene056590 | MS.gene070564 | 0.812633 | 3.84E-51 | -1.69E-46 |
| MS.gene056591 | MS.gene070564 | 0.813204 | 2.88E-51 | -1.69E-46 |
| MS.gene056856 | MS.gene070564 | 0.802266 | 6.09E-49 | -1.69E-46 |
| MS.gene058603 | MS.gene070564 | 0.820956 | 5.21E-53 | -1.69E-46 |
| MS.gene059953 | MS.gene070564 | 0.825475 | 4.59E-54 | -1.69E-46 |
| MS.gene059955 | MS.gene070564 | 0.839209 | 1.82E-57 | -1.69E-46 |
| MS.gene060593 | MS.gene070564 | 0.805362 | 1.39E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene070564 | MS.gene032888 | PPI |
| MS.gene78610 | MS.gene070564 | PPI |
| MS.gene042064 | MS.gene070564 | PPI |
| MS.gene032887 | MS.gene070564 | PPI |
| MS.gene43462 | MS.gene070564 | PPI |
| MS.gene070564 | MS.gene77424 | PPI |
| MS.gene012776 | MS.gene070564 | PPI |
| MS.gene070564 | MS.gene53154 | PPI |
| MS.gene012777 | MS.gene070564 | PPI |
| MS.gene85927 | MS.gene070564 | PPI |
| MS.gene43463 | MS.gene070564 | PPI |
| MS.gene042063 | MS.gene070564 | PPI |
| MS.gene95067 | MS.gene070564 | PPI |
| MS.gene55353 | MS.gene070564 | PPI |
| MS.gene95066 | MS.gene070564 | PPI |
| MS.gene032888 | MS.gene070564 | PPI |
| MS.gene77425 | MS.gene070564 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070564.t1 | MTR_5g040430 | 91.556 | 225 | 19 | 0 | 1 | 225 | 1 | 225 | 7.37e-155 | 428 |
| MS.gene070564.t1 | MTR_4g019780 | 49.302 | 215 | 103 | 2 | 7 | 221 | 8 | 216 | 1.72e-66 | 204 |
| MS.gene070564.t1 | MTR_4g019790 | 42.793 | 222 | 119 | 4 | 1 | 221 | 1 | 215 | 2.64e-59 | 186 |
| MS.gene070564.t1 | MTR_7g065660 | 41.441 | 222 | 122 | 3 | 1 | 221 | 1 | 215 | 4.86e-56 | 178 |
| MS.gene070564.t1 | MTR_1g090070 | 39.910 | 223 | 125 | 4 | 4 | 223 | 6 | 222 | 3.05e-55 | 176 |
| MS.gene070564.t1 | MTR_7g065630 | 40.541 | 222 | 120 | 4 | 1 | 221 | 19 | 229 | 6.76e-55 | 176 |
| MS.gene070564.t1 | MTR_7g065600 | 40.991 | 222 | 123 | 3 | 1 | 221 | 1 | 215 | 1.24e-54 | 174 |
| MS.gene070564.t1 | MTR_1g090060 | 41.743 | 218 | 123 | 2 | 6 | 223 | 2 | 215 | 1.64e-54 | 174 |
| MS.gene070564.t1 | MTR_3g099757 | 42.991 | 214 | 116 | 2 | 8 | 221 | 6 | 213 | 1.70e-54 | 174 |
| MS.gene070564.t1 | MTR_7g065720 | 40.807 | 223 | 123 | 3 | 1 | 221 | 1 | 216 | 3.39e-54 | 173 |
| MS.gene070564.t1 | MTR_7g065590 | 41.204 | 216 | 119 | 3 | 7 | 221 | 7 | 215 | 4.18e-54 | 173 |
| MS.gene070564.t1 | MTR_7g065750 | 42.152 | 223 | 120 | 4 | 1 | 221 | 1 | 216 | 6.21e-54 | 172 |
| MS.gene070564.t1 | MTR_4g059730 | 44.076 | 211 | 112 | 5 | 14 | 223 | 11 | 216 | 1.68e-53 | 171 |
| MS.gene070564.t1 | MTR_7g065230 | 42.342 | 222 | 115 | 4 | 1 | 221 | 1 | 210 | 5.54e-53 | 170 |
| MS.gene070564.t1 | MTR_8g087410 | 41.981 | 212 | 117 | 2 | 5 | 216 | 4 | 209 | 6.55e-53 | 170 |
| MS.gene070564.t1 | MTR_7g065740 | 39.910 | 223 | 125 | 3 | 1 | 221 | 1 | 216 | 1.70e-52 | 169 |
| MS.gene070564.t1 | MTR_7g065290 | 40.991 | 222 | 123 | 3 | 1 | 221 | 1 | 215 | 2.52e-52 | 169 |
| MS.gene070564.t1 | MTR_7g065265 | 40.367 | 218 | 122 | 3 | 1 | 217 | 1 | 211 | 4.34e-52 | 168 |
| MS.gene070564.t1 | MTR_2g070150 | 37.619 | 210 | 124 | 2 | 5 | 214 | 2 | 204 | 2.40e-50 | 163 |
| MS.gene070564.t1 | MTR_8g087425 | 39.171 | 217 | 126 | 2 | 5 | 221 | 3 | 213 | 4.39e-50 | 163 |
| MS.gene070564.t1 | MTR_7g065270 | 39.908 | 218 | 123 | 3 | 1 | 217 | 1 | 211 | 5.45e-50 | 162 |
| MS.gene070564.t1 | MTR_1g090100 | 40.741 | 216 | 124 | 2 | 8 | 223 | 3 | 214 | 7.46e-50 | 162 |
| MS.gene070564.t1 | MTR_2g070060 | 36.323 | 223 | 132 | 3 | 5 | 224 | 2 | 217 | 1.71e-49 | 161 |
| MS.gene070564.t1 | MTR_6g080440 | 40.284 | 211 | 120 | 2 | 6 | 216 | 2 | 206 | 4.83e-49 | 160 |
| MS.gene070564.t1 | MTR_7g065640 | 40.090 | 222 | 107 | 4 | 1 | 221 | 1 | 197 | 9.41e-49 | 159 |
| MS.gene070564.t1 | MTR_2g070180 | 36.019 | 211 | 128 | 2 | 5 | 215 | 2 | 205 | 2.06e-48 | 158 |
| MS.gene070564.t1 | MTR_2g070130 | 36.323 | 223 | 132 | 3 | 5 | 224 | 2 | 217 | 3.63e-48 | 158 |
| MS.gene070564.t1 | MTR_7g065260 | 40.094 | 212 | 121 | 2 | 1 | 212 | 8 | 213 | 3.81e-48 | 158 |
| MS.gene070564.t1 | MTR_1g090150 | 39.252 | 214 | 125 | 3 | 7 | 220 | 3 | 211 | 5.52e-47 | 155 |
| MS.gene070564.t1 | MTR_1g090090 | 39.381 | 226 | 122 | 5 | 6 | 222 | 2 | 221 | 7.67e-47 | 154 |
| MS.gene070564.t1 | MTR_2g070200 | 36.364 | 209 | 125 | 3 | 5 | 212 | 2 | 203 | 9.06e-47 | 154 |
| MS.gene070564.t1 | MTR_2g070120 | 39.151 | 212 | 120 | 4 | 5 | 215 | 2 | 205 | 9.73e-47 | 154 |
| MS.gene070564.t1 | MTR_7g065710 | 37.981 | 208 | 117 | 4 | 8 | 214 | 5 | 201 | 2.43e-46 | 153 |
| MS.gene070564.t1 | MTR_2g070140 | 37.129 | 202 | 120 | 2 | 21 | 222 | 2 | 196 | 4.58e-46 | 152 |
| MS.gene070564.t1 | MTR_7g065680 | 40.278 | 216 | 117 | 4 | 7 | 221 | 7 | 211 | 6.21e-45 | 149 |
| MS.gene070564.t1 | MTR_8g061950 | 39.091 | 220 | 130 | 3 | 6 | 222 | 4 | 222 | 8.33e-45 | 149 |
| MS.gene070564.t1 | MTR_5g037380 | 35.814 | 215 | 134 | 3 | 5 | 216 | 3 | 216 | 2.28e-44 | 148 |
| MS.gene070564.t1 | MTR_7g065700 | 42.927 | 205 | 105 | 5 | 7 | 210 | 7 | 200 | 2.56e-44 | 148 |
| MS.gene070564.t1 | MTR_2g070210 | 35.111 | 225 | 134 | 4 | 5 | 224 | 2 | 219 | 3.81e-44 | 147 |
| MS.gene070564.t1 | MTR_1g115195 | 35.321 | 218 | 132 | 4 | 6 | 220 | 2 | 213 | 4.98e-43 | 144 |
| MS.gene070564.t1 | MTR_2g070070 | 35.426 | 223 | 134 | 3 | 5 | 224 | 2 | 217 | 5.84e-43 | 144 |
| MS.gene070564.t1 | MTR_8g056940 | 36.098 | 205 | 124 | 2 | 5 | 209 | 2 | 199 | 1.39e-42 | 143 |
| MS.gene070564.t1 | MTR_2g070110 | 36.771 | 223 | 130 | 5 | 5 | 224 | 2 | 216 | 1.61e-41 | 140 |
| MS.gene070564.t1 | MTR_5g076900 | 36.364 | 209 | 124 | 4 | 8 | 215 | 7 | 207 | 1.72e-40 | 138 |
| MS.gene070564.t1 | MTR_3g467420 | 32.692 | 208 | 132 | 3 | 6 | 212 | 8 | 208 | 5.08e-40 | 137 |
| MS.gene070564.t1 | MTR_3g467430 | 33.333 | 213 | 132 | 4 | 1 | 212 | 5 | 208 | 5.60e-39 | 134 |
| MS.gene070564.t1 | MTR_0186s0030 | 45.185 | 135 | 74 | 0 | 5 | 139 | 2 | 136 | 5.82e-38 | 129 |
| MS.gene070564.t1 | MTR_4g124130 | 39.785 | 186 | 102 | 4 | 6 | 187 | 3 | 182 | 3.50e-36 | 127 |
| MS.gene070564.t1 | MTR_2g072120 | 44.286 | 70 | 39 | 0 | 51 | 120 | 2 | 71 | 2.33e-20 | 82.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070564.t1 | AT1G74590 | 43.182 | 220 | 124 | 1 | 1 | 220 | 1 | 219 | 3.99e-67 | 206 |
| MS.gene070564.t1 | AT5G62480 | 44.000 | 225 | 122 | 3 | 1 | 221 | 1 | 225 | 1.48e-65 | 203 |
| MS.gene070564.t1 | AT3G09270 | 41.593 | 226 | 125 | 3 | 1 | 225 | 1 | 220 | 3.20e-57 | 181 |
| MS.gene070564.t1 | AT2G29450 | 45.161 | 217 | 107 | 6 | 4 | 216 | 3 | 211 | 2.59e-53 | 171 |
| MS.gene070564.t1 | AT2G29490 | 38.326 | 227 | 131 | 4 | 1 | 225 | 1 | 220 | 4.49e-52 | 168 |
| MS.gene070564.t1 | AT2G29480 | 39.111 | 225 | 128 | 4 | 1 | 223 | 1 | 218 | 9.37e-52 | 167 |
| MS.gene070564.t1 | AT2G29460 | 36.889 | 225 | 133 | 4 | 1 | 223 | 1 | 218 | 4.43e-51 | 165 |
| MS.gene070564.t1 | AT2G29440 | 40.271 | 221 | 123 | 5 | 4 | 222 | 3 | 216 | 6.15e-51 | 165 |
| MS.gene070564.t1 | AT2G29420 | 37.615 | 218 | 128 | 3 | 5 | 221 | 7 | 217 | 3.67e-50 | 163 |
| MS.gene070564.t1 | AT5G62480 | 37.778 | 225 | 110 | 4 | 1 | 221 | 1 | 199 | 2.27e-48 | 158 |
| MS.gene070564.t1 | AT2G29470 | 37.004 | 227 | 133 | 5 | 1 | 224 | 1 | 220 | 1.16e-47 | 157 |
| MS.gene070564.t1 | AT5G62480 | 37.333 | 225 | 110 | 4 | 1 | 221 | 1 | 198 | 1.36e-47 | 156 |
| MS.gene070564.t1 | AT1G78380 | 34.434 | 212 | 132 | 2 | 5 | 216 | 2 | 206 | 1.11e-46 | 154 |
| MS.gene070564.t1 | AT1G78320 | 34.742 | 213 | 132 | 2 | 7 | 219 | 4 | 209 | 1.38e-45 | 151 |
| MS.gene070564.t1 | AT1G17180 | 35.849 | 212 | 129 | 2 | 5 | 216 | 2 | 206 | 1.90e-45 | 151 |
| MS.gene070564.t1 | AT1G10370 | 36.986 | 219 | 137 | 1 | 5 | 222 | 3 | 221 | 4.07e-45 | 150 |
| MS.gene070564.t1 | AT1G78340 | 36.872 | 179 | 108 | 1 | 5 | 183 | 2 | 175 | 6.79e-44 | 147 |
| MS.gene070564.t1 | AT1G17170 | 36.279 | 215 | 129 | 3 | 5 | 219 | 2 | 208 | 1.70e-43 | 145 |
| MS.gene070564.t1 | AT1G10360 | 36.916 | 214 | 132 | 3 | 5 | 216 | 3 | 215 | 2.49e-43 | 145 |
| MS.gene070564.t1 | AT1G78370 | 32.057 | 209 | 135 | 2 | 9 | 217 | 6 | 207 | 2.55e-43 | 145 |
| MS.gene070564.t1 | AT1G53680 | 37.991 | 229 | 130 | 5 | 1 | 224 | 1 | 222 | 4.57e-43 | 145 |
| MS.gene070564.t1 | AT3G43800 | 34.513 | 226 | 137 | 5 | 1 | 223 | 1 | 218 | 4.29e-42 | 142 |
| MS.gene070564.t1 | AT1G69930 | 35.714 | 210 | 133 | 2 | 8 | 216 | 13 | 221 | 5.98e-42 | 142 |
| MS.gene070564.t1 | AT1G27130 | 35.023 | 217 | 137 | 3 | 4 | 217 | 3 | 218 | 8.94e-42 | 142 |
| MS.gene070564.t1 | AT1G59670 | 36.364 | 220 | 126 | 5 | 4 | 215 | 3 | 216 | 2.10e-40 | 138 |
| MS.gene070564.t1 | AT1G78360 | 33.333 | 216 | 136 | 4 | 5 | 219 | 2 | 210 | 1.11e-39 | 136 |
| MS.gene070564.t1 | AT1G17190 | 31.604 | 212 | 138 | 2 | 5 | 216 | 3 | 207 | 4.38e-39 | 134 |
| MS.gene070564.t1 | AT1G69920 | 33.491 | 212 | 137 | 3 | 2 | 210 | 29 | 239 | 6.54e-39 | 135 |
| MS.gene070564.t1 | AT1G59700 | 37.037 | 216 | 132 | 4 | 4 | 216 | 3 | 217 | 1.22e-37 | 131 |
| MS.gene070564.t1 | AT1G27140 | 33.036 | 224 | 145 | 3 | 4 | 223 | 3 | 225 | 2.34e-36 | 128 |
| MS.gene070564.t1 | AT1G78320 | 29.108 | 213 | 117 | 3 | 7 | 219 | 4 | 182 | 4.39e-30 | 110 |
Find 59 sgRNAs with CRISPR-Local
Find 105 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAATACATCGATGAAATT+TGG | 0.107039 | 5.3:+28331507 | MS.gene070564:CDS |
| TCACCAGAGGATGCTCCTTT+AGG | 0.227371 | 5.3:-28332526 | None:intergenic |
| AAGGTATACCCTTCACTTTA+AGG | 0.271772 | 5.3:-28331361 | None:intergenic |
| CTTCCTTTATGCACGAGAAT+TGG | 0.274141 | 5.3:-28331458 | None:intergenic |
| CACCAGAGGATGCTCCTTTA+GGG | 0.280200 | 5.3:-28332525 | None:intergenic |
| TTCTCTCTTCTCCTCGAGAT+TGG | 0.307184 | 5.3:-28332283 | None:intergenic |
| TGAATACATCGATGAAATTT+GGG | 0.316149 | 5.3:+28331508 | MS.gene070564:CDS |
| AGGATCCTCTGGCAAGAGTT+TGG | 0.331001 | 5.3:-28331540 | None:intergenic |
| GCGTTTCGCCATTGAAGAAT+TGG | 0.361541 | 5.3:-28332382 | None:intergenic |
| GTACAATCCTGTCCATAAAA+AGG | 0.369292 | 5.3:+28331433 | MS.gene070564:CDS |
| TTCCTTTATGCACGAGAATT+GGG | 0.383723 | 5.3:-28331457 | None:intergenic |
| AGGTATACCCTTCACTTTAA+GGG | 0.391035 | 5.3:-28331360 | None:intergenic |
| GGATCCTCTGGCAAGAGTTT+GGG | 0.395079 | 5.3:-28331539 | None:intergenic |
| AAGTTGTGTTGTTGGCAAAC+TGG | 0.400482 | 5.3:+28331306 | MS.gene070564:CDS |
| CGGACTTTAGCCCTTTGATA+AGG | 0.406040 | 5.3:-28331560 | None:intergenic |
| GCCCTTTGATAAGGATCCTC+TGG | 0.413467 | 5.3:-28331551 | None:intergenic |
| GTCCCAATTCTCGTGCATAA+AGG | 0.433737 | 5.3:+28331455 | MS.gene070564:CDS |
| TCACTTGTTTCTTCATCCAT+TGG | 0.440197 | 5.3:-28331284 | None:intergenic |
| ATACTTGGGGTGTTTGAAGA+AGG | 0.446735 | 5.3:+28332332 | MS.gene070564:CDS |
| TTTAAGGGCTAGTTCTACTT+TGG | 0.446812 | 5.3:-28331345 | None:intergenic |
| TACTTGGGGTGTTTGAAGAA+GGG | 0.448054 | 5.3:+28332333 | MS.gene070564:CDS |
| AAGCAATTCCAATTCTTCAA+TGG | 0.449699 | 5.3:+28332374 | MS.gene070564:CDS |
| AACAAGTGAAGTTGTGTTGT+TGG | 0.453636 | 5.3:+28331298 | MS.gene070564:CDS |
| TGGAATATGCGGTAGCTACT+AGG | 0.468988 | 5.3:-28332263 | None:intergenic |
| CATGTGGGTGAATGCCCTAA+AGG | 0.473273 | 5.3:+28332511 | MS.gene070564:CDS |
| TGCCCTAAAGGAGCATCCTC+TGG | 0.481677 | 5.3:+28332523 | MS.gene070564:CDS |
| TACTTTCTGATCAAAGTAGT+TGG | 0.483393 | 5.3:-28331591 | None:intergenic |
| TCCTCCTCCACATACTTGTA+AGG | 0.491365 | 5.3:-28331380 | None:intergenic |
| GAAGACATGGAAGAGATACT+TGG | 0.494020 | 5.3:+28332317 | MS.gene070564:CDS |
| GGCCTCTTGAAATGCTTCAT+AGG | 0.494435 | 5.3:-28332444 | None:intergenic |
| ACTTGGGGTGTTTGAAGAAG+GGG | 0.504475 | 5.3:+28332334 | MS.gene070564:CDS |
| AGTTGTGTTGTTGGCAAACT+GGG | 0.513784 | 5.3:+28331307 | MS.gene070564:CDS |
| GCCAGAGGATCCTTATCAAA+GGG | 0.523043 | 5.3:+28331550 | MS.gene070564:CDS |
| AAAGAAAGCTGCAGAAGACA+TGG | 0.529969 | 5.3:+28332304 | MS.gene070564:CDS |
| GGTGTTTGAAGAAGGGGTTA+AGG | 0.532545 | 5.3:+28332340 | MS.gene070564:CDS |
| ACCTTACAAGTATGTGGAGG+AGG | 0.533258 | 5.3:+28331379 | MS.gene070564:CDS |
| AAGACATGGAAGAGATACTT+GGG | 0.539650 | 5.3:+28332318 | MS.gene070564:CDS |
| CGTGAAGACCTCTACATCGC+AGG | 0.547982 | 5.3:-28332465 | None:intergenic |
| GGGTATACCTTACAAGTATG+TGG | 0.549194 | 5.3:+28331373 | MS.gene070564:CDS |
| ACTTCCTCCTCATGACAAGT+TGG | 0.553330 | 5.3:+28332556 | MS.gene070564:CDS |
| AGAACTAGCCCTTAAAGTGA+AGG | 0.562510 | 5.3:+28331352 | MS.gene070564:CDS |
| TCCTCCTCATGACAAGTTGG+TGG | 0.565432 | 5.3:+28332559 | MS.gene070564:CDS |
| CACCTATGAAGCATTTCAAG+AGG | 0.567937 | 5.3:+28332442 | MS.gene070564:CDS |
| TGCCAGAGGATCCTTATCAA+AGG | 0.569024 | 5.3:+28331549 | MS.gene070564:CDS |
| GCTAGGACTCCTCGAAATTG+TGG | 0.571810 | 5.3:+28332403 | MS.gene070564:CDS |
| GAACTAGCCCTTAAAGTGAA+GGG | 0.575201 | 5.3:+28331353 | MS.gene070564:CDS |
| TTCTTCAATGGCGAAACGCT+AGG | 0.575394 | 5.3:+28332386 | MS.gene070564:CDS |
| TCAAGAGGCCTGCGATGTAG+AGG | 0.579483 | 5.3:+28332457 | MS.gene070564:CDS |
| GCCACCAACTTGTCATGAGG+AGG | 0.584492 | 5.3:-28332560 | None:intergenic |
| AAAGGAGCATCCTCTGGTGA+AGG | 0.590106 | 5.3:+28332529 | MS.gene070564:CDS |
| TTGGCCACCAACTTGTCATG+AGG | 0.608148 | 5.3:-28332563 | None:intergenic |
| TCGCGGCTACCACAATTTCG+AGG | 0.622866 | 5.3:-28332412 | None:intergenic |
| TTCATAGGTGCAAGAAGTCG+CGG | 0.631066 | 5.3:-28332429 | None:intergenic |
| CTCCTCGAGATTGGAATATG+CGG | 0.637949 | 5.3:-28332274 | None:intergenic |
| GGAAGTGTTTCCTTCACCAG+AGG | 0.655838 | 5.3:-28332539 | None:intergenic |
| TACCGCATATTCCAATCTCG+AGG | 0.668331 | 5.3:+28332272 | MS.gene070564:CDS |
| TAGTCCCAAACTCTTGCCAG+AGG | 0.676520 | 5.3:+28331535 | MS.gene070564:CDS |
| TATACCTTACAAGTATGTGG+AGG | 0.715787 | 5.3:+28331376 | MS.gene070564:CDS |
| AGACATGGAAGAGATACTTG+GGG | 0.746381 | 5.3:+28332319 | MS.gene070564:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACATATAATTTGTTTTTTTA+AGG | + | chr5.3:28331712-28331731 | MS.gene070564:intron | 10.0% |
| !!! | CATATAATTTGTTTTTTTAA+GGG | + | chr5.3:28331713-28331732 | MS.gene070564:intron | 10.0% |
| !! | ATGTAAATATAAGTAGAAAA+GGG | - | chr5.3:28331696-28331715 | None:intergenic | 15.0% |
| !! | TATGTAAATATAAGTAGAAA+AGG | - | chr5.3:28331697-28331716 | None:intergenic | 15.0% |
| !!! | TCCAATAATACTTTTTATTT+TGG | - | chr5.3:28332585-28332604 | None:intergenic | 15.0% |
| !! | AATAGTTATGGAAGTAAAAT+AGG | + | chr5.3:28331981-28332000 | MS.gene070564:intron | 20.0% |
| !! | ATTGGTAAGTTACTTAAAAT+TGG | - | chr5.3:28331964-28331983 | None:intergenic | 20.0% |
| !!! | GCCAAAATAAAAAGTATTAT+TGG | + | chr5.3:28332581-28332600 | MS.gene070564:CDS | 20.0% |
| !!! | TATTTTACTTCCATAACTAT+TGG | - | chr5.3:28331982-28332001 | None:intergenic | 20.0% |
| !!! | TGTCTTCTCTTAATAATTTT+AGG | - | chr5.3:28331858-28331877 | None:intergenic | 20.0% |
| ! | AAGTAACTTACCAATAGTTA+TGG | + | chr5.3:28331969-28331988 | MS.gene070564:intron | 25.0% |
| ! | AAGTTAATTAACAAGTCTCT+AGG | - | chr5.3:28332207-28332226 | None:intergenic | 25.0% |
| ! | AGTTAATTAACAAGTCTCTA+GGG | - | chr5.3:28332206-28332225 | None:intergenic | 25.0% |
| ! | TGAATACATCGATGAAATTT+GGG | + | chr5.3:28331508-28331527 | MS.gene070564:CDS | 25.0% |
| ! | TTGAATACATCGATGAAATT+TGG | + | chr5.3:28331507-28331526 | MS.gene070564:CDS | 25.0% |
| !! | CATTTTCTTGTTAGTCAATT+TGG | + | chr5.3:28331830-28331849 | MS.gene070564:intron | 25.0% |
| !! | TTTTATCTAACCACTCAAAT+TGG | + | chr5.3:28332052-28332071 | MS.gene070564:intron | 25.0% |
| !!! | TAGAGACTTGTTAATTAACT+TGG | + | chr5.3:28332206-28332225 | MS.gene070564:intron | 25.0% |
| AAACCTTAATCCATCATATC+TGG | - | chr5.3:28332034-28332053 | None:intergenic | 30.0% | |
| AAGCAATTCCAATTCTTCAA+TGG | + | chr5.3:28332374-28332393 | MS.gene070564:CDS | 30.0% | |
| ACTTTATCCACTAAAAGAAC+AGG | + | chr5.3:28332083-28332102 | MS.gene070564:intron | 30.0% | |
| CTTTATCCACTAAAAGAACA+GGG | + | chr5.3:28332084-28332103 | MS.gene070564:intron | 30.0% | |
| TACTTTCTGATCAAAGTAGT+TGG | - | chr5.3:28331594-28331613 | None:intergenic | 30.0% | |
| TTCACCCACATGAAAAATTT+CGG | - | chr5.3:28332503-28332522 | None:intergenic | 30.0% | |
| ! | AAAAATTTCGGCATCTTTTC+AGG | - | chr5.3:28332491-28332510 | None:intergenic | 30.0% |
| !! | TTCTTTTAGTGGATAAAGTG+AGG | - | chr5.3:28332082-28332101 | None:intergenic | 30.0% |
| AACAAGTGAAGTTGTGTTGT+TGG | + | chr5.3:28331298-28331317 | MS.gene070564:CDS | 35.0% | |
| AACCTATTAGCCAGATATGA+TGG | + | chr5.3:28332021-28332040 | MS.gene070564:intron | 35.0% | |
| AAGACATGGAAGAGATACTT+GGG | + | chr5.3:28332318-28332337 | MS.gene070564:CDS | 35.0% | |
| AAGGTATACCCTTCACTTTA+AGG | - | chr5.3:28331364-28331383 | None:intergenic | 35.0% | |
| AGGTATACCCTTCACTTTAA+GGG | - | chr5.3:28331363-28331382 | None:intergenic | 35.0% | |
| ATCCATCATATCTGGCTAAT+AGG | - | chr5.3:28332026-28332045 | None:intergenic | 35.0% | |
| GTACAATCCTGTCCATAAAA+AGG | + | chr5.3:28331433-28331452 | MS.gene070564:CDS | 35.0% | |
| TAGGGATAAAATTGGCTACT+AGG | - | chr5.3:28332188-28332207 | None:intergenic | 35.0% | |
| TATACCTTACAAGTATGTGG+AGG | + | chr5.3:28331376-28331395 | MS.gene070564:CDS | 35.0% | |
| TTCCTTTATGCACGAGAATT+GGG | - | chr5.3:28331460-28331479 | None:intergenic | 35.0% | |
| ! | AGATGCCGAAATTTTTCATG+TGG | + | chr5.3:28332495-28332514 | MS.gene070564:CDS | 35.0% |
| ! | CAAGTCTCTAGGGATAAAAT+TGG | - | chr5.3:28332196-28332215 | None:intergenic | 35.0% |
| ! | GATGCCGAAATTTTTCATGT+GGG | + | chr5.3:28332496-28332515 | MS.gene070564:CDS | 35.0% |
| !! | TAGCCAGATATGATGGATTA+AGG | + | chr5.3:28332028-28332047 | MS.gene070564:intron | 35.0% |
| !! | TTTAAGGGCTAGTTCTACTT+TGG | - | chr5.3:28331348-28331367 | None:intergenic | 35.0% |
| AAAGAAAGCTGCAGAAGACA+TGG | + | chr5.3:28332304-28332323 | MS.gene070564:CDS | 40.0% | |
| ACTAAAAGAACAGGGTGGAT+AGG | + | chr5.3:28332092-28332111 | MS.gene070564:intron | 40.0% | |
| AGAACTAGCCCTTAAAGTGA+AGG | + | chr5.3:28331352-28331371 | MS.gene070564:CDS | 40.0% | |
| AGACATGGAAGAGATACTTG+GGG | + | chr5.3:28332319-28332338 | MS.gene070564:CDS | 40.0% | |
| CAAAGTAGTTGGCCCAAAAA+CGG | - | chr5.3:28331583-28331602 | None:intergenic | 40.0% | |
| CTAAAAGAACAGGGTGGATA+GGG | + | chr5.3:28332093-28332112 | MS.gene070564:intron | 40.0% | |
| CTTCCTTTATGCACGAGAAT+TGG | - | chr5.3:28331461-28331480 | None:intergenic | 40.0% | |
| GAACTAGCCCTTAAAGTGAA+GGG | + | chr5.3:28331353-28331372 | MS.gene070564:CDS | 40.0% | |
| GAAGACATGGAAGAGATACT+TGG | + | chr5.3:28332317-28332336 | MS.gene070564:CDS | 40.0% | |
| GGGTATACCTTACAAGTATG+TGG | + | chr5.3:28331373-28331392 | MS.gene070564:CDS | 40.0% | |
| TATCCACTAAAAGAACAGGG+TGG | + | chr5.3:28332087-28332106 | MS.gene070564:intron | 40.0% | |
| ! | AAAGGGCTAAAGTCCGTTTT+TGG | + | chr5.3:28331567-28331586 | MS.gene070564:CDS | 40.0% |
| ! | AAGTTGTGTTGTTGGCAAAC+TGG | + | chr5.3:28331306-28331325 | MS.gene070564:CDS | 40.0% |
| ! | AGTTGTGTTGTTGGCAAACT+GGG | + | chr5.3:28331307-28331326 | MS.gene070564:CDS | 40.0% |
| ! | ATACTTGGGGTGTTTGAAGA+AGG | + | chr5.3:28332332-28332351 | MS.gene070564:CDS | 40.0% |
| ! | ATTGGGACCTTTTTATGGAC+AGG | - | chr5.3:28331443-28331462 | None:intergenic | 40.0% |
| ! | CACCTATGAAGCATTTCAAG+AGG | + | chr5.3:28332442-28332461 | MS.gene070564:CDS | 40.0% |
| ! | CGAGAATTGGGACCTTTTTA+TGG | - | chr5.3:28331448-28331467 | None:intergenic | 40.0% |
| ! | GAAGTAAAATAGGTTGAGCC+CGG | + | chr5.3:28331991-28332010 | MS.gene070564:intron | 40.0% |
| ! | TACTTGGGGTGTTTGAAGAA+GGG | + | chr5.3:28332333-28332352 | MS.gene070564:CDS | 40.0% |
| ! | TATCCACCCTGTTCTTTTAG+TGG | - | chr5.3:28332093-28332112 | None:intergenic | 40.0% |
| !! | AAGGGCTAAAGTCCGTTTTT+GGG | + | chr5.3:28331568-28331587 | MS.gene070564:CDS | 40.0% |
| ACCTTACAAGTATGTGGAGG+AGG | + | chr5.3:28331379-28331398 | MS.gene070564:CDS | 45.0% | |
| ACTTCCTCCTCATGACAAGT+TGG | + | chr5.3:28332556-28332575 | MS.gene070564:CDS | 45.0% | |
| CGGACTTTAGCCCTTTGATA+AGG | - | chr5.3:28331563-28331582 | None:intergenic | 45.0% | |
| CTCCTCGAGATTGGAATATG+CGG | - | chr5.3:28332277-28332296 | None:intergenic | 45.0% | |
| GCCAGAGGATCCTTATCAAA+GGG | + | chr5.3:28331550-28331569 | MS.gene070564:CDS | 45.0% | |
| GCGTTTCGCCATTGAAGAAT+TGG | - | chr5.3:28332385-28332404 | None:intergenic | 45.0% | |
| GGCCTCTTGAAATGCTTCAT+AGG | - | chr5.3:28332447-28332466 | None:intergenic | 45.0% | |
| GGTGTTTGAAGAAGGGGTTA+AGG | + | chr5.3:28332340-28332359 | MS.gene070564:CDS | 45.0% | |
| GTCCCAATTCTCGTGCATAA+AGG | + | chr5.3:28331455-28331474 | MS.gene070564:CDS | 45.0% | |
| TACCGCATATTCCAATCTCG+AGG | + | chr5.3:28332272-28332291 | MS.gene070564:CDS | 45.0% | |
| TCCTCCTCCACATACTTGTA+AGG | - | chr5.3:28331383-28331402 | None:intergenic | 45.0% | |
| TGCCAGAGGATCCTTATCAA+AGG | + | chr5.3:28331549-28331568 | MS.gene070564:CDS | 45.0% | |
| TGGAATATGCGGTAGCTACT+AGG | - | chr5.3:28332266-28332285 | None:intergenic | 45.0% | |
| TTCATAGGTGCAAGAAGTCG+CGG | - | chr5.3:28332432-28332451 | None:intergenic | 45.0% | |
| TTCTCTCTTCTCCTCGAGAT+TGG | - | chr5.3:28332286-28332305 | None:intergenic | 45.0% | |
| TTCTTCAATGGCGAAACGCT+AGG | + | chr5.3:28332386-28332405 | MS.gene070564:CDS | 45.0% | |
| ! | ACTTGGGGTGTTTGAAGAAG+GGG | + | chr5.3:28332334-28332353 | MS.gene070564:CDS | 45.0% |
| AAGAACAGGGTGGATAGGGT+TGG | + | chr5.3:28332097-28332116 | MS.gene070564:intron | 50.0% | |
| AGGATCCTCTGGCAAGAGTT+TGG | - | chr5.3:28331543-28331562 | None:intergenic | 50.0% | |
| CACCAGAGGATGCTCCTTTA+GGG | - | chr5.3:28332528-28332547 | None:intergenic | 50.0% | |
| CATGTGGGTGAATGCCCTAA+AGG | + | chr5.3:28332511-28332530 | MS.gene070564:CDS | 50.0% | |
| GCCCTTTGATAAGGATCCTC+TGG | - | chr5.3:28331554-28331573 | None:intergenic | 50.0% | |
| GCTAATAGGTTAAACCCGCC+GGG | - | chr5.3:28332012-28332031 | None:intergenic | 50.0% | |
| GCTAGGACTCCTCGAAATTG+TGG | + | chr5.3:28332403-28332422 | MS.gene070564:CDS | 50.0% | |
| GGATCCTCTGGCAAGAGTTT+GGG | - | chr5.3:28331542-28331561 | None:intergenic | 50.0% | |
| GGCTAATAGGTTAAACCCGC+CGG | - | chr5.3:28332013-28332032 | None:intergenic | 50.0% | |
| GTGAGGCGAACCAATTTGAG+TGG | - | chr5.3:28332065-28332084 | None:intergenic | 50.0% | |
| TAGTCCCAAACTCTTGCCAG+AGG | + | chr5.3:28331535-28331554 | MS.gene070564:CDS | 50.0% | |
| TCACCAGAGGATGCTCCTTT+AGG | - | chr5.3:28332529-28332548 | None:intergenic | 50.0% | |
| TTGGCCACCAACTTGTCATG+AGG | - | chr5.3:28332566-28332585 | None:intergenic | 50.0% | |
| ! | GTAAAATAGGTTGAGCCCGG+CGG | + | chr5.3:28331994-28332013 | MS.gene070564:intron | 50.0% |
| ! | TAAAATAGGTTGAGCCCGGC+GGG | + | chr5.3:28331995-28332014 | MS.gene070564:intron | 50.0% |
| ! | TCCTCCTCATGACAAGTTGG+TGG | + | chr5.3:28332559-28332578 | MS.gene070564:CDS | 50.0% |
| !! | AAAGGAGCATCCTCTGGTGA+AGG | + | chr5.3:28332529-28332548 | MS.gene070564:CDS | 50.0% |
| !! | GGAAGTGTTTCCTTCACCAG+AGG | - | chr5.3:28332542-28332561 | None:intergenic | 50.0% |
| CGTGAAGACCTCTACATCGC+AGG | - | chr5.3:28332468-28332487 | None:intergenic | 55.0% | |
| GCCACCAACTTGTCATGAGG+AGG | - | chr5.3:28332563-28332582 | None:intergenic | 55.0% | |
| TCAAGAGGCCTGCGATGTAG+AGG | + | chr5.3:28332457-28332476 | MS.gene070564:CDS | 55.0% | |
| TCGCGGCTACCACAATTTCG+AGG | - | chr5.3:28332415-28332434 | None:intergenic | 55.0% | |
| !! | GGTGGATAGGGTTGGCTCAT+CGG | + | chr5.3:28332105-28332124 | MS.gene070564:intron | 55.0% |
| !! | GTGGATAGGGTTGGCTCATC+GGG | + | chr5.3:28332106-28332125 | MS.gene070564:intron | 55.0% |
| !! | TGCCCTAAAGGAGCATCCTC+TGG | + | chr5.3:28332523-28332542 | MS.gene070564:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 28331287 | 28332610 | 28331287 | ID=MS.gene070564 |
| chr5.3 | mRNA | 28331287 | 28332610 | 28331287 | ID=MS.gene070564.t1;Parent=MS.gene070564 |
| chr5.3 | exon | 28331287 | 28331610 | 28331287 | ID=MS.gene070564.t1.exon1;Parent=MS.gene070564.t1 |
| chr5.3 | CDS | 28331287 | 28331610 | 28331287 | ID=cds.MS.gene070564.t1;Parent=MS.gene070564.t1 |
| chr5.3 | exon | 28332257 | 28332610 | 28332257 | ID=MS.gene070564.t1.exon2;Parent=MS.gene070564.t1 |
| chr5.3 | CDS | 28332257 | 28332610 | 28332257 | ID=cds.MS.gene070564.t1;Parent=MS.gene070564.t1 |
| Gene Sequence |
| Protein sequence |
