AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07981


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07981.t1 MTR_6g038750 95.597 159 7 0 1 159 552 710 5.00e-100 302
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07981.t1 AT1G71810 48.750 160 73 3 1 159 541 692 1.63e-38 138

Find 39 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATCTTCTTCGGTCATTAA+AGG 0.214414 6.3:+32195907 None:intergenic
CGCCTTATGCTATTACTATC+AGG 0.254646 6.3:-32195862 MS.gene07981:CDS
TGCACTTGGGCTTGCTTCCT+TGG 0.320437 6.3:-32195974 MS.gene07981:CDS
CCGAGTACAGTATTTGATTC+TGG 0.326284 6.3:+32194997 None:intergenic
AATAATTGCTGCTGGGATTC+CGG 0.357575 6.3:+32194762 None:intergenic
TAAGTTGACAATATCTTCTT+CGG 0.390382 6.3:+32195897 None:intergenic
ATTTCAATTGCCAGCTAACT+TGG 0.391080 6.3:-32194742 MS.gene07981:CDS
AAGAAGATATTGTCAACTTA+AGG 0.428840 6.3:-32195894 MS.gene07981:CDS
CTGCTGCGAGAACTATCAAA+AGG 0.443736 6.3:-32194698 MS.gene07981:CDS
TTTCTGTTAGTGTAAAAGAA+AGG 0.455354 6.3:+32196341 None:intergenic
AACTGTTGAACCAAGTTAGC+TGG 0.464113 6.3:+32194732 None:intergenic
GCAATTGAAATAATTGCTGC+TGG 0.469883 6.3:+32194754 None:intergenic
TACAGTATTTGATTCTGGTC+GGG 0.473562 6.3:+32195002 None:intergenic
AACAGGAAGAATGTCTTGAA+TGG 0.494577 6.3:+32194941 None:intergenic
TCAGGAATGACGGAAAGAAC+AGG 0.508934 6.3:+32194924 None:intergenic
GATAGTTCTCGCAGCAGCGC+GGG 0.517491 6.3:+32194704 None:intergenic
TCTTGCAGCAGCTGTTTCCA+AGG 0.528924 6.3:+32195957 None:intergenic
CCCCTGATAGTAATAGCATA+AGG 0.543212 6.3:+32195860 None:intergenic
ATGTAGGGTTTAGATGCACT+TGG 0.550374 6.3:-32195988 MS.gene07981:intron
CCAGAATCAAATACTGTACT+CGG 0.551398 6.3:-32194997 MS.gene07981:CDS
GCCTTATGCTATTACTATCA+GGG 0.556826 6.3:-32195861 MS.gene07981:CDS
AGATTCGAAAATGGTTATGA+AGG 0.557498 6.3:-32196368 None:intergenic
TATATTTACCTCAGGAATGA+CGG 0.557901 6.3:+32194914 None:intergenic
ATGTAAATTTCAGCTCCTAC+CGG 0.564826 6.3:-32194781 MS.gene07981:intron
ATAGTAATAGCATAAGGCGT+TGG 0.567548 6.3:+32195866 None:intergenic
GTACAGTATTTGATTCTGGT+CGG 0.577930 6.3:+32195001 None:intergenic
TGTTCTTTCCGTCATTCCTG+AGG 0.588868 6.3:-32194922 MS.gene07981:intron
CAATTGAAATAATTGCTGCT+GGG 0.591793 6.3:+32194755 None:intergenic
TGCTGCGAGAACTATCAAAA+GGG 0.593150 6.3:-32194697 MS.gene07981:CDS
TGTAGGGTTTAGATGCACTT+GGG 0.596142 6.3:-32195987 MS.gene07981:intron
TAATAGCATAAGGCGTTGGA+AGG 0.598040 6.3:+32195870 None:intergenic
GAAAAGAAGAAGTTACTGAA+AGG 0.608276 6.3:+32195931 None:intergenic
TGTCTTATAGGGAGAGGCAA+AGG 0.610421 6.3:-32195030 MS.gene07981:intron
AATTGTTCAAGAATTTGCCA+AGG 0.611754 6.3:-32196203 MS.gene07981:intron
ATTGCTGCTGGGATTCCGGT+AGG 0.637646 6.3:+32194766 None:intergenic
TGTTGCAGGGTGGATTTGTG+AGG 0.637741 6.3:-32196231 MS.gene07981:intron
CCTTATGCTATTACTATCAG+GGG 0.647224 6.3:-32195860 MS.gene07981:CDS
TGATAGTTCTCGCAGCAGCG+CGG 0.673188 6.3:+32194703 None:intergenic
GAATCAAATACTGTACTCGG+AGG 0.741867 6.3:-32194994 MS.gene07981:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGATTGATTTTTCTTTT+AGG - chr6.3:32195291-32195310 MS.gene07981:intron 15.0%
!! ATTAAACTAATAGTCTACAA+TGG + chr6.3:32194923-32194942 None:intergenic 20.0%
!! TATGAAAATGAAACGATAAT+TGG - chr6.3:32195943-32195962 MS.gene07981:CDS 20.0%
!!! AAAAGCATCTAAATTGTTAA+TGG + chr6.3:32194745-32194764 None:intergenic 20.0%
!!! AAAGCATCTAAATTGTTAAT+GGG + chr6.3:32194744-32194763 None:intergenic 20.0%
! AAGAAGATATTGTCAACTTA+AGG - chr6.3:32195150-32195169 MS.gene07981:intron 25.0%
! AAGCATCTAAATTGTTAATG+GGG + chr6.3:32194743-32194762 None:intergenic 25.0%
! AGATTAGAAATGAAGAAAGA+AGG + chr6.3:32194994-32195013 None:intergenic 25.0%
! AGTTTACATTATGATTGTAG+TGG + chr6.3:32195740-32195759 None:intergenic 25.0%
! ATCATGTAAGTACCATATTT+CGG + chr6.3:32195320-32195339 None:intergenic 25.0%
! TATCATGAAAACAAATACCT+TGG + chr6.3:32194861-32194880 None:intergenic 25.0%
! TTTCTGTTAGTGTAAAAGAA+AGG + chr6.3:32194706-32194725 None:intergenic 25.0%
!! TAAGTTGACAATATCTTCTT+CGG + chr6.3:32195150-32195169 None:intergenic 25.0%
!! TTCTTTTACACTAACAGAAA+AGG - chr6.3:32194706-32194725 MS.gene07981:CDS 25.0%
!!! TACGATTTTTCCTTTGTTTT+GGG + chr6.3:32195682-32195701 None:intergenic 25.0%
!!! TTACGATTTTTCCTTTGTTT+TGG + chr6.3:32195683-32195702 None:intergenic 25.0%
AATTGTTCAAGAATTTGCCA+AGG - chr6.3:32194841-32194860 MS.gene07981:intron 30.0%
ATACTCAAACACATTGCAAT+GGG + chr6.3:32195880-32195899 None:intergenic 30.0%
ATATCTTCTTCGGTCATTAA+AGG + chr6.3:32195140-32195159 None:intergenic 30.0%
CAAATGCTTATATTTACCTC+AGG + chr6.3:32196141-32196160 None:intergenic 30.0%
CAATTGAAATAATTGCTGCT+GGG + chr6.3:32196292-32196311 None:intergenic 30.0%
CTTTCATGATAATTGTTGCA+GGG - chr6.3:32194800-32194819 MS.gene07981:intron 30.0%
GAAAAGAAGAAGTTACTGAA+AGG + chr6.3:32195116-32195135 None:intergenic 30.0%
TATAATATATGACGCTCAGT+GGG + chr6.3:32195643-32195662 None:intergenic 30.0%
TATATTTACCTCAGGAATGA+CGG + chr6.3:32196133-32196152 None:intergenic 30.0%
TCACATAATCAGGAGAATAA+AGG + chr6.3:32195575-32195594 None:intergenic 30.0%
TCATGAAAGACATGTTAGAA+AGG + chr6.3:32194789-32194808 None:intergenic 30.0%
TCATGTAAGTACCATATTTC+GGG + chr6.3:32195319-32195338 None:intergenic 30.0%
TCTTTCATGATAATTGTTGC+AGG - chr6.3:32194799-32194818 MS.gene07981:intron 30.0%
TTAATCCTTTCACCTTCATT+AGG - chr6.3:32194967-32194986 MS.gene07981:CDS 30.0%
! CTAACTTTTACCCAAAACAA+AGG - chr6.3:32195669-32195688 MS.gene07981:intron 30.0%
!! TGTAAACTTCTAAAGCTTCT+AGG - chr6.3:32195752-32195771 MS.gene07981:intron 30.0%
AACAGGAAGAATGTCTTGAA+TGG + chr6.3:32196106-32196125 None:intergenic 35.0%
AAGAAAGAAGGACCTAATGA+AGG + chr6.3:32194982-32195001 None:intergenic 35.0%
ACTTTCCGTCAATGTCTTAT+AGG - chr6.3:32196002-32196021 MS.gene07981:intron 35.0%
AGATATGCACTCACATAATC+AGG + chr6.3:32195585-32195604 None:intergenic 35.0%
ATAGTAATAGCATAAGGCGT+TGG + chr6.3:32195181-32195200 None:intergenic 35.0%
ATGTAAATTTCAGCTCCTAC+CGG - chr6.3:32196263-32196282 MS.gene07981:intron 35.0%
ATGTAAGTACCATATTTCGG+GGG + chr6.3:32195317-32195336 None:intergenic 35.0%
ATTTCAATTGCCAGCTAACT+TGG - chr6.3:32196302-32196321 MS.gene07981:intron 35.0%
CATGTAAGTACCATATTTCG+GGG + chr6.3:32195318-32195337 None:intergenic 35.0%
CCAGAATCAAATACTGTACT+CGG - chr6.3:32196047-32196066 MS.gene07981:intron 35.0%
CCTTATGCTATTACTATCAG+GGG - chr6.3:32195184-32195203 MS.gene07981:intron 35.0%
CGACTGTAGTAGTATAATAG+TGG - chr6.3:32195900-32195919 MS.gene07981:CDS 35.0%
CTTTCCGTCAATGTCTTATA+GGG - chr6.3:32196003-32196022 MS.gene07981:intron 35.0%
GAAAGGCAGTAAAAACTTCA+GGG - chr6.3:32195774-32195793 MS.gene07981:intron 35.0%
GATACTCAAACACATTGCAA+TGG + chr6.3:32195881-32195900 None:intergenic 35.0%
GCAATTGAAATAATTGCTGC+TGG + chr6.3:32196293-32196312 None:intergenic 35.0%
GCCTTATGCTATTACTATCA+GGG - chr6.3:32195183-32195202 MS.gene07981:intron 35.0%
GTACAGTATTTGATTCTGGT+CGG + chr6.3:32196046-32196065 None:intergenic 35.0%
GTATAATATATGACGCTCAG+TGG + chr6.3:32195644-32195663 None:intergenic 35.0%
GTCATAAGCAATTTCACATC+TGG - chr6.3:32196207-32196226 MS.gene07981:CDS 35.0%
TAAAGTCTTTGTCATGCTTC+CGG - chr6.3:32194879-32194898 MS.gene07981:intron 35.0%
TACAGTATTTGATTCTGGTC+GGG + chr6.3:32196045-32196064 None:intergenic 35.0%
TACTCAAACACATTGCAATG+GGG + chr6.3:32195879-32195898 None:intergenic 35.0%
TGTAGTAGTATAATAGTGGC+TGG - chr6.3:32195904-32195923 MS.gene07981:CDS 35.0%
TTGCTTTCGATCCAAATGTA+GGG - chr6.3:32195041-32195060 MS.gene07981:intron 35.0%
TTTATCCATGGTGATTTCCA+TGG + chr6.3:32195371-32195390 None:intergenic 35.0%
TTTGCTTTCGATCCAAATGT+AGG - chr6.3:32195040-32195059 MS.gene07981:intron 35.0%
! ATCGAAGTCTTCTTTTCTTC+TGG + chr6.3:32195210-32195229 None:intergenic 35.0%
!! AAGCAAATTTTAACGCTCAG+AGG + chr6.3:32195027-32195046 None:intergenic 35.0%
!! ACTTCTAAAGCTTCTAGGAA+AGG - chr6.3:32195757-32195776 MS.gene07981:intron 35.0%
!! AGCAAATTTTAACGCTCAGA+GGG + chr6.3:32195026-32195045 None:intergenic 35.0%
AACTGTTGAACCAAGTTAGC+TGG + chr6.3:32196315-32196334 None:intergenic 40.0%
AAGGACCTAATGAAGGTGAA+AGG + chr6.3:32194975-32194994 None:intergenic 40.0%
AATAATTGCTGCTGGGATTC+CGG + chr6.3:32196285-32196304 None:intergenic 40.0%
ATGTAGGGTTTAGATGCACT+TGG - chr6.3:32195056-32195075 MS.gene07981:intron 40.0%
CCCCTGATAGTAATAGCATA+AGG + chr6.3:32195187-32195206 None:intergenic 40.0%
CCGAGTACAGTATTTGATTC+TGG + chr6.3:32196050-32196069 None:intergenic 40.0%
CGCCTTATGCTATTACTATC+AGG - chr6.3:32195182-32195201 MS.gene07981:intron 40.0%
CTCTCCCTATAAGACATTGA+CGG + chr6.3:32196010-32196029 None:intergenic 40.0%
GAATCAAATACTGTACTCGG+AGG - chr6.3:32196050-32196069 MS.gene07981:intron 40.0%
GGAAAGGCAGTAAAAACTTC+AGG - chr6.3:32195773-32195792 MS.gene07981:intron 40.0%
GGATAAAGCTTGAAGCTCTA+AGG - chr6.3:32195384-32195403 MS.gene07981:intron 40.0%
GTGCATCTAAACCCTACATT+TGG + chr6.3:32195055-32195074 None:intergenic 40.0%
TCATGATAATTGTTGCAGGG+TGG - chr6.3:32194803-32194822 MS.gene07981:intron 40.0%
TGCTGCGAGAACTATCAAAA+GGG - chr6.3:32196347-32196366 MS.gene07981:CDS 40.0%
TGTAGGGTTTAGATGCACTT+GGG - chr6.3:32195057-32195076 MS.gene07981:intron 40.0%
! AGAGCTTCAAGCTTTATCCA+TGG + chr6.3:32195383-32195402 None:intergenic 40.0%
! CGTTGATGAATGAGTTTCCA+CGG - chr6.3:32195809-32195828 MS.gene07981:intron 40.0%
!! AGCTGTTTTGTCAGTGTTAG+TGG + chr6.3:32196185-32196204 None:intergenic 40.0%
!! GCAAATTTTAACGCTCAGAG+GGG + chr6.3:32195025-32195044 None:intergenic 40.0%
!! TAATAGCATAAGGCGTTGGA+AGG + chr6.3:32195177-32195196 None:intergenic 40.0%
!! TTCTAAGATTGAAACCGCCA+TGG - chr6.3:32195351-32195370 MS.gene07981:intron 40.0%
ACAATGGCCTAGAATGCATG+AGG + chr6.3:32194907-32194926 None:intergenic 45.0%
ATCCATGGTGATTTCCATGG+CGG + chr6.3:32195368-32195387 None:intergenic 45.0%
CAATGTAGCAATGTAGTCCG+TGG + chr6.3:32195829-32195848 None:intergenic 45.0%
CGTCAATGTCTTATAGGGAG+AGG - chr6.3:32196008-32196027 MS.gene07981:intron 45.0%
CTGCTGCGAGAACTATCAAA+AGG - chr6.3:32196346-32196365 MS.gene07981:CDS 45.0%
TGGGCATTCACACGTATTAG+AGG + chr6.3:32195624-32195643 None:intergenic 45.0%
TGTCTTATAGGGAGAGGCAA+AGG - chr6.3:32196014-32196033 MS.gene07981:intron 45.0%
TGTTCTTTCCGTCATTCCTG+AGG - chr6.3:32196122-32196141 MS.gene07981:intron 45.0%
! TCAGGAATGACGGAAAGAAC+AGG + chr6.3:32196123-32196142 None:intergenic 45.0%
! TCTTTTAGGCCCCCGAAATA+TGG - chr6.3:32195305-32195324 MS.gene07981:intron 45.0%
AACCGCCATGGAAATCACCA+TGG - chr6.3:32195363-32195382 MS.gene07981:intron 50.0%
! TCTTGCAGCAGCTGTTTCCA+AGG + chr6.3:32195090-32195109 None:intergenic 50.0%
! TGTTGCAGGGTGGATTTGTG+AGG - chr6.3:32194813-32194832 MS.gene07981:intron 50.0%
ATTGCTGCTGGGATTCCGGT+AGG + chr6.3:32196281-32196300 None:intergenic 55.0%
CTACATTGCGTAGTGCGCGT+AGG - chr6.3:32195841-32195860 MS.gene07981:CDS 55.0%
GCCTAGAATGCATGAGGTGC+CGG + chr6.3:32194901-32194920 None:intergenic 55.0%
TCCGGCACCTCATGCATTCT+AGG - chr6.3:32194897-32194916 MS.gene07981:intron 55.0%
TGATAGTTCTCGCAGCAGCG+CGG + chr6.3:32196344-32196363 None:intergenic 55.0%
! TGCACTTGGGCTTGCTTCCT+TGG - chr6.3:32195070-32195089 MS.gene07981:intron 55.0%
GATAGTTCTCGCAGCAGCGC+GGG + chr6.3:32196343-32196362 None:intergenic 60.0%
GCGTAGTGCGCGTAGGTGCT+AGG - chr6.3:32195848-32195867 MS.gene07981:CDS 65.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 32194686 32196380 32194686 ID=MS.gene07981
chr6.3 mRNA 32194686 32196380 32194686 ID=MS.gene07981.t1;Parent=MS.gene07981
chr6.3 exon 32196339 32196380 32196339 ID=MS.gene07981.t1.exon1;Parent=MS.gene07981.t1
chr6.3 CDS 32196339 32196380 32196339 ID=cds.MS.gene07981.t1;Parent=MS.gene07981.t1
chr6.3 exon 32196204 32196245 32196204 ID=MS.gene07981.t1.exon2;Parent=MS.gene07981.t1
chr6.3 CDS 32196204 32196245 32196204 ID=cds.MS.gene07981.t1;Parent=MS.gene07981.t1
chr6.3 exon 32195834 32196004 32195834 ID=MS.gene07981.t1.exon3;Parent=MS.gene07981.t1
chr6.3 CDS 32195834 32196004 32195834 ID=cds.MS.gene07981.t1;Parent=MS.gene07981.t1
chr6.3 exon 32194923 32195042 32194923 ID=MS.gene07981.t1.exon4;Parent=MS.gene07981.t1
chr6.3 CDS 32194923 32195042 32194923 ID=cds.MS.gene07981.t1;Parent=MS.gene07981.t1
chr6.3 exon 32194686 32194790 32194686 ID=MS.gene07981.t1.exon5;Parent=MS.gene07981.t1
chr6.3 CDS 32194686 32194790 32194686 ID=cds.MS.gene07981.t1;Parent=MS.gene07981.t1
Gene Sequence

>MS.gene07981

ATGGTTATGAAGGAAATCCTTTCTTTTACACTAACAGAAAAGGTACATATTGTTCCCCATTAACAATTTAGATGCTTTTTTGCAACTGCATTTAACTTCTCCTTTCTAACATGTCTTTCATGATAATTGTTGCAGGGTGGATTTGTGAGGAAAATAATTGTTCAAGAATTTGCCAAGGTATTTGTTTTCATGATAAAGTCTTTGTCATGCTTCCGGCACCTCATGCATTCTAGGCCATTGTAGACTATTAGTTTAATATTTTTTTTTTATACGATATTAGTTTAATCCTTTCACCTTCATTAGGTCCTTCTTTCTTCATTTCTAATCTAGTTTTCACCCCTCTGAGCGTTAAAATTTGCTTTCGATCCAAATGTAGGGTTTAGATGCACTTGGGCTTGCTTCCTTGGAAACAGCTGCTGCAAGAGTTCCTTTCAGTAACTTCTTCTTTTCACCTTTAATGACCGAAGAAGATATTGTCAACTTAAGGACCTTCCAACGCCTTATGCTATTACTATCAGGGGCCAGAAGAAAAGAAGACTTCGATATTGTAAGTTTTGTAGTTTACGCAAAAACTATATCTTTGCATTGTTATATTTTTTTATCATTTTTGATTGATTTTTCTTTTAGGCCCCCGAAATATGGTACTTACATGATTACAGTGCTTGTTCTAAGATTGAAACCGCCATGGAAATCACCATGGATAAAGCTTGAAGCTCTAAGGAAGAAGAAAGAGAATGTATTGATTCAATTAAGAAAACAGTTACAAGAGTAGCTTATATAGAAGCTAAGATCTGTAACTAACTACATGCAATACAAATGAAATGTAAATGAAAGTGAAATTACAAATGCACAAATGCACTTAAGCTAACTTGCGCTCTAAGTAACTCCTTTATTCTCCTGATTATGTGAGTGCATATCTTTCTCTTCAATGCTTTCCTCTAATACGTGTGAATGCCCACTGAGCGTCATATATTATACTGAAACTAACTTTTACCCAAAACAAAGGAAAAATCGTAAAACTGTTTCAAATTAAAATTTTGAACACACTCTTCCACTACAATCATAATGTAAACTTCTAAAGCTTCTAGGAAAGGCAGTAAAAACTTCAGGGAAGTTATGTTTTCGTTGATGAATGAGTTTCCACGGACTACATTGCTACATTGCGTAGTGCGCGTAGGTGCTAGGCAAAACCCCATTGCAATGTGTTTGAGTATCGACTGTAGTAGTATAATAGTGGCTGGTACAGTGAGACAAATATATGAAAATGAAACGATAATTGGATTTATTATTTAACTTGTTTATGACTAATTGCAAGTACTTTCCGTCAATGTCTTATAGGGAGAGGCAAAGGCTAGTCCCGACCAGAATCAAATACTGTACTCGGAGGAGCTGTCGCTAGTTTTAAATCAATTTGACTCCATTCAAGACATTCTTCCTGTTCTTTCCGTCATTCCTGAGGTAAATATAAGCATTTGTCTATTATTTAAGATATGTAACCACTAACACTGACAAAACAGCTAAGTCATAAGCAATTTCACATCTGGACATTCTTTATTACAGCTGATGCATGACTATATATGTAAATTTCAGCTCCTACCGGAATCCCAGCAGCAATTATTTCAATTGCCAGCTAACTTGGTTCAACAGTTAATATCCCGCGCTGCTGCGAGAACTATCAAAAGGGTGTTTTTGTGA

Protein sequence

>MS.gene07981.t1

MVMKEILSFTLTEKGGFVRKIIVQEFAKGLDALGLASLETAAARVPFSNFFFSPLMTEEDIVNLRTFQRLMLLLSGARRKEDFDIGEAKASPDQNQILYSEELSLVLNQFDSIQDILPVLSVIPELLPESQQQLFQLPANLVQQLISRAAARTIKRVFL