Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20856.t1 | XP_003591401.1 | 96.9 | 131 | 4 | 0 | 1 | 131 | 1 | 131 | 5.20E-62 | 246.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20856.t1 | Q9LZP9 | 53.3 | 135 | 55 | 3 | 1 | 131 | 1 | 131 | 2.9e-29 | 129.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20856.t1 | B7FGI5 | 96.9 | 131 | 4 | 0 | 1 | 131 | 1 | 131 | 3.7e-62 | 246.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene20856 | 0.802741 | 4.86E-49 | -1.69E-46 |
| MS.gene049171 | MS.gene20856 | 0.833743 | 4.48E-56 | -1.69E-46 |
| MS.gene049257 | MS.gene20856 | 0.815047 | 1.13E-51 | -1.69E-46 |
| MS.gene049609 | MS.gene20856 | 0.801761 | 7.73E-49 | -1.69E-46 |
| MS.gene050133 | MS.gene20856 | -0.801136 | 1.04E-48 | -1.69E-46 |
| MS.gene05081 | MS.gene20856 | 0.844033 | 9.71E-59 | -1.69E-46 |
| MS.gene051244 | MS.gene20856 | 0.806412 | 8.33E-50 | -1.69E-46 |
| MS.gene051838 | MS.gene20856 | 0.831191 | 1.92E-55 | -1.69E-46 |
| MS.gene052134 | MS.gene20856 | 0.829903 | 3.97E-55 | -1.69E-46 |
| MS.gene052135 | MS.gene20856 | 0.800962 | 1.13E-48 | -1.69E-46 |
| MS.gene052469 | MS.gene20856 | 0.837515 | 4.97E-57 | -1.69E-46 |
| MS.gene052606 | MS.gene20856 | -0.804308 | 2.30E-49 | -1.69E-46 |
| MS.gene053330 | MS.gene20856 | 0.831263 | 1.84E-55 | -1.69E-46 |
| MS.gene054343 | MS.gene20856 | 0.851415 | 9.01E-61 | -1.69E-46 |
| MS.gene054344 | MS.gene20856 | 0.812023 | 5.22E-51 | -1.69E-46 |
| MS.gene054884 | MS.gene20856 | -0.858194 | 9.74E-63 | -1.69E-46 |
| MS.gene055206 | MS.gene20856 | 0.805314 | 1.42E-49 | -1.69E-46 |
| MS.gene055289 | MS.gene20856 | 0.804017 | 2.64E-49 | -1.69E-46 |
| MS.gene055370 | MS.gene20856 | 0.843616 | 1.26E-58 | -1.69E-46 |
| MS.gene055539 | MS.gene20856 | 0.826425 | 2.73E-54 | -1.69E-46 |
| MS.gene055731 | MS.gene20856 | -0.808942 | 2.42E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene20856 | 0.800864 | 1.18E-48 | -1.69E-46 |
| MS.gene056351 | MS.gene20856 | 0.803686 | 3.10E-49 | -1.69E-46 |
| MS.gene056633 | MS.gene20856 | 0.871718 | 5.52E-67 | -1.69E-46 |
| MS.gene056664 | MS.gene20856 | 0.809051 | 2.29E-50 | -1.69E-46 |
| MS.gene056697 | MS.gene20856 | -0.809183 | 2.15E-50 | -1.69E-46 |
| MS.gene057196 | MS.gene20856 | 0.836768 | 7.71E-57 | -1.69E-46 |
| MS.gene057332 | MS.gene20856 | 0.856535 | 3.01E-62 | -1.69E-46 |
| MS.gene057658 | MS.gene20856 | 0.833885 | 4.12E-56 | -1.69E-46 |
| MS.gene058544 | MS.gene20856 | 0.814336 | 1.62E-51 | -1.69E-46 |
| MS.gene058746 | MS.gene20856 | 0.864386 | 1.26E-64 | -1.69E-46 |
| MS.gene058838 | MS.gene20856 | 0.846717 | 1.82E-59 | -1.69E-46 |
| MS.gene059023 | MS.gene20856 | 0.830758 | 2.45E-55 | -1.69E-46 |
| MS.gene05914 | MS.gene20856 | 0.841292 | 5.19E-58 | -1.69E-46 |
| MS.gene059512 | MS.gene20856 | 0.848962 | 4.39E-60 | -1.69E-46 |
| MS.gene059855 | MS.gene20856 | -0.8151 | 1.10E-51 | -1.69E-46 |
| MS.gene060087 | MS.gene20856 | -0.807328 | 5.33E-50 | -1.69E-46 |
| MS.gene06092 | MS.gene20856 | 0.806933 | 6.47E-50 | -1.69E-46 |
| MS.gene061231 | MS.gene20856 | 0.848521 | 5.81E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20856.t1 | MTR_1g087030 | 96.947 | 131 | 4 | 0 | 1 | 131 | 1 | 131 | 3.59e-92 | 262 |
| MS.gene20856.t1 | MTR_7g109520 | 60.769 | 130 | 45 | 4 | 3 | 130 | 2 | 127 | 2.55e-48 | 151 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20856.t1 | AT3G62410 | 53.333 | 135 | 55 | 3 | 1 | 131 | 1 | 131 | 1.43e-43 | 139 |
| MS.gene20856.t1 | AT2G47400 | 62.745 | 102 | 33 | 4 | 32 | 131 | 26 | 124 | 8.06e-34 | 114 |
| MS.gene20856.t1 | AT1G76560 | 48.611 | 72 | 35 | 1 | 62 | 131 | 63 | 134 | 7.78e-18 | 74.3 |
Find 47 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGCTCTCTTCCACCTTCC+CGG | 0.302904 | 1.3:-61577924 | None:intergenic |
| TGACTGCACCGGTCCATCTT+TGG | 0.344117 | 1.3:-61577850 | None:intergenic |
| CAAGCATCACCGGACATAAC+CGG | 0.380356 | 1.3:+61577905 | MS.gene20856:CDS |
| AAGTCTCAGGCCATCAGGTC+TGG | 0.407675 | 1.3:+61577809 | MS.gene20856:CDS |
| TTCAAAAGTCTCAGGCCATC+AGG | 0.429143 | 1.3:+61577804 | MS.gene20856:CDS |
| TCCACCTTCCCGGTTATGTC+CGG | 0.434096 | 1.3:-61577914 | None:intergenic |
| TGGACCGGTGCAGTCAGTTC+CGG | 0.447899 | 1.3:+61577857 | MS.gene20856:CDS |
| GAGCATAAAGAGTGCGGAAG+AGG | 0.462834 | 1.3:+61577940 | MS.gene20856:CDS |
| ATAGTATCATCAGTCTTCAT+AGG | 0.466900 | 1.3:-61578119 | None:intergenic |
| GGCGGCGTGGGACGAGGTAG+AGG | 0.472117 | 1.3:+61577997 | MS.gene20856:CDS |
| TGTTCATGCTCAACCAAAGA+TGG | 0.486584 | 1.3:+61577837 | MS.gene20856:CDS |
| ATGTGTGGCGGCGTGGGACG+AGG | 0.494667 | 1.3:+61577991 | MS.gene20856:CDS |
| AGTGCGGAAGAGGCGTGTGC+AGG | 0.498117 | 1.3:+61577950 | MS.gene20856:CDS |
| GGAATACTGCAAGGACAATC+CGG | 0.499926 | 1.3:+61578078 | MS.gene20856:CDS |
| GTGCAGCGGCAAGCCATGCA+AGG | 0.500755 | 1.3:+61578026 | MS.gene20856:CDS |
| CGGTGAATGTGTGGCGGCGT+GGG | 0.504208 | 1.3:+61577985 | MS.gene20856:CDS |
| GGTCCCTTGAAAATTACTCT+AGG | 0.509125 | 1.3:-61577773 | None:intergenic |
| AGAGTCACTTCAAAAGTCTC+AGG | 0.515969 | 1.3:+61577796 | MS.gene20856:CDS |
| TCGACCAGTGCAAGCATCAC+CGG | 0.524706 | 1.3:+61577895 | MS.gene20856:CDS |
| GTCCCTTGAAAATTACTCTA+GGG | 0.529441 | 1.3:-61577772 | None:intergenic |
| TGCAGTTATGGCAGCAACAA+TGG | 0.530066 | 1.3:+61577730 | None:intergenic |
| CGCCACTAGCGGTGAATGTG+TGG | 0.535540 | 1.3:+61577976 | MS.gene20856:CDS |
| TGCAGTCAGTTCCGGTAGGA+TGG | 0.539303 | 1.3:+61577865 | MS.gene20856:CDS |
| CGCCACACATTCACCGCTAG+TGG | 0.552220 | 1.3:-61577978 | None:intergenic |
| GTTATGGCAGCAACAATGGC+TGG | 0.554458 | 1.3:+61577734 | None:intergenic |
| ATGTCCGGTGATGCTTGCAC+TGG | 0.555683 | 1.3:-61577899 | None:intergenic |
| AAGCATCACCGGACATAACC+GGG | 0.568302 | 1.3:+61577906 | MS.gene20856:CDS |
| ATGCTCAACCAAAGATGGAC+CGG | 0.572661 | 1.3:+61577842 | MS.gene20856:CDS |
| TGCAGCGGCAAGCCATGCAA+GGG | 0.573901 | 1.3:+61578027 | MS.gene20856:CDS |
| GAACACTGGTCCAGACCTGA+TGG | 0.573903 | 1.3:-61577819 | None:intergenic |
| TCCTTGCAGTATTCCTCGAG+AGG | 0.580542 | 1.3:-61578070 | None:intergenic |
| AAAGACGTCGGACCCTCTCG+AGG | 0.580550 | 1.3:+61578057 | MS.gene20856:CDS |
| GCGGTGAATGTGTGGCGGCG+TGG | 0.592717 | 1.3:+61577984 | MS.gene20856:CDS |
| TCGAATGCTTACCATCCTAC+CGG | 0.601933 | 1.3:-61577876 | None:intergenic |
| CCTTGCAGTATTCCTCGAGA+GGG | 0.604324 | 1.3:-61578069 | None:intergenic |
| ACCGGACATAACCGGGAAGG+TGG | 0.605824 | 1.3:+61577913 | MS.gene20856:CDS |
| GTACGACACTCATTAGCCTC+CGG | 0.606135 | 1.3:-61578097 | None:intergenic |
| CTTTGGTTGAGCATGAACAC+TGG | 0.608780 | 1.3:-61577833 | None:intergenic |
| CCCTCTCGAGGAATACTGCA+AGG | 0.623920 | 1.3:+61578069 | MS.gene20856:CDS |
| CACTAGCGGTGAATGTGTGG+CGG | 0.630714 | 1.3:+61577979 | MS.gene20856:CDS |
| CCGGTGCAGTCAGTTCCGGT+AGG | 0.639198 | 1.3:+61577861 | MS.gene20856:CDS |
| CCTACCGGAACTGACTGCAC+CGG | 0.656038 | 1.3:-61577861 | None:intergenic |
| GGAAGAGAGCATAAAGAGTG+CGG | 0.657638 | 1.3:+61577934 | MS.gene20856:CDS |
| TGTGCAGGAGACGCCACTAG+CGG | 0.665675 | 1.3:+61577965 | MS.gene20856:CDS |
| ATCACCGGACATAACCGGGA+AGG | 0.669449 | 1.3:+61577910 | MS.gene20856:CDS |
| GGTAGAGGAACTGAGTGCAG+CGG | 0.687487 | 1.3:+61578012 | MS.gene20856:CDS |
| ATACTGCAAGGACAATCCGG+AGG | 0.732199 | 1.3:+61578081 | MS.gene20856:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGGGATAAGAAAAAGACGT+CGG | + | chr1.3:61578045-61578064 | MS.gene20856:CDS | 35.0% | |
| GTCCCTTGAAAATTACTCTA+GGG | - | chr1.3:61577775-61577794 | None:intergenic | 35.0% | |
| ! | AAGCCCTAGAGTAATTTTCA+AGG | + | chr1.3:61577769-61577788 | MS.gene20856:CDS | 35.0% |
| ! | AGCCCTAGAGTAATTTTCAA+GGG | + | chr1.3:61577770-61577789 | MS.gene20856:CDS | 35.0% |
| ! | TCTTTTTCTTATCCCTTGCA+TGG | - | chr1.3:61578042-61578061 | None:intergenic | 35.0% |
| AGAGTCACTTCAAAAGTCTC+AGG | + | chr1.3:61577796-61577815 | MS.gene20856:CDS | 40.0% | |
| GGTCCCTTGAAAATTACTCT+AGG | - | chr1.3:61577776-61577795 | None:intergenic | 40.0% | |
| TGTTCATGCTCAACCAAAGA+TGG | + | chr1.3:61577837-61577856 | MS.gene20856:CDS | 40.0% | |
| !!! | TGAGACTTTTGAAGTGACTC+TGG | - | chr1.3:61577797-61577816 | None:intergenic | 40.0% |
| ATGCTCAACCAAAGATGGAC+CGG | + | chr1.3:61577842-61577861 | MS.gene20856:CDS | 45.0% | |
| CTTTGGTTGAGCATGAACAC+TGG | - | chr1.3:61577836-61577855 | None:intergenic | 45.0% | |
| GGAATACTGCAAGGACAATC+CGG | + | chr1.3:61578078-61578097 | MS.gene20856:CDS | 45.0% | |
| TCGAATGCTTACCATCCTAC+CGG | - | chr1.3:61577879-61577898 | None:intergenic | 45.0% | |
| TTCAAAAGTCTCAGGCCATC+AGG | + | chr1.3:61577804-61577823 | MS.gene20856:CDS | 45.0% | |
| ! | GGAAGAGAGCATAAAGAGTG+CGG | + | chr1.3:61577934-61577953 | MS.gene20856:CDS | 45.0% |
| AAGCATCACCGGACATAACC+GGG | + | chr1.3:61577906-61577925 | MS.gene20856:CDS | 50.0% | |
| ATACTGCAAGGACAATCCGG+AGG | + | chr1.3:61578081-61578100 | MS.gene20856:CDS | 50.0% | |
| CAAGCATCACCGGACATAAC+CGG | + | chr1.3:61577905-61577924 | MS.gene20856:CDS | 50.0% | |
| CCTTGCAGTATTCCTCGAGA+GGG | - | chr1.3:61578072-61578091 | None:intergenic | 50.0% | |
| TATGCTCTCTTCCACCTTCC+CGG | - | chr1.3:61577927-61577946 | None:intergenic | 50.0% | |
| TCCTTGCAGTATTCCTCGAG+AGG | - | chr1.3:61578073-61578092 | None:intergenic | 50.0% | |
| ! | GAGCATAAAGAGTGCGGAAG+AGG | + | chr1.3:61577940-61577959 | MS.gene20856:CDS | 50.0% |
| ! | GTACGACACTCATTAGCCTC+CGG | - | chr1.3:61578100-61578119 | None:intergenic | 50.0% |
| AAGTCTCAGGCCATCAGGTC+TGG | + | chr1.3:61577809-61577828 | MS.gene20856:CDS | 55.0% | |
| ATCACCGGACATAACCGGGA+AGG | + | chr1.3:61577910-61577929 | MS.gene20856:CDS | 55.0% | |
| ATGTCCGGTGATGCTTGCAC+TGG | - | chr1.3:61577902-61577921 | None:intergenic | 55.0% | |
| CACTAGCGGTGAATGTGTGG+CGG | + | chr1.3:61577979-61577998 | MS.gene20856:CDS | 55.0% | |
| CCCTCTCGAGGAATACTGCA+AGG | + | chr1.3:61578069-61578088 | MS.gene20856:CDS | 55.0% | |
| GAACACTGGTCCAGACCTGA+TGG | - | chr1.3:61577822-61577841 | None:intergenic | 55.0% | |
| GGTAGAGGAACTGAGTGCAG+CGG | + | chr1.3:61578012-61578031 | MS.gene20856:CDS | 55.0% | |
| TCCACCTTCCCGGTTATGTC+CGG | - | chr1.3:61577917-61577936 | None:intergenic | 55.0% | |
| TCGACCAGTGCAAGCATCAC+CGG | + | chr1.3:61577895-61577914 | MS.gene20856:CDS | 55.0% | |
| TGCAGTCAGTTCCGGTAGGA+TGG | + | chr1.3:61577865-61577884 | MS.gene20856:CDS | 55.0% | |
| ! | TGACTGCACCGGTCCATCTT+TGG | - | chr1.3:61577853-61577872 | None:intergenic | 55.0% |
| AAAGACGTCGGACCCTCTCG+AGG | + | chr1.3:61578057-61578076 | MS.gene20856:CDS | 60.0% | |
| ACCGGACATAACCGGGAAGG+TGG | + | chr1.3:61577913-61577932 | MS.gene20856:CDS | 60.0% | |
| CCTACCGGAACTGACTGCAC+CGG | - | chr1.3:61577864-61577883 | None:intergenic | 60.0% | |
| CGCCACACATTCACCGCTAG+TGG | - | chr1.3:61577981-61578000 | None:intergenic | 60.0% | |
| CGCCACTAGCGGTGAATGTG+TGG | + | chr1.3:61577976-61577995 | MS.gene20856:CDS | 60.0% | |
| TGCAGCGGCAAGCCATGCAA+GGG | + | chr1.3:61578027-61578046 | MS.gene20856:CDS | 60.0% | |
| TGGACCGGTGCAGTCAGTTC+CGG | + | chr1.3:61577857-61577876 | MS.gene20856:CDS | 60.0% | |
| TGTGCAGGAGACGCCACTAG+CGG | + | chr1.3:61577965-61577984 | MS.gene20856:CDS | 60.0% | |
| CCGGTGCAGTCAGTTCCGGT+AGG | + | chr1.3:61577861-61577880 | MS.gene20856:CDS | 65.0% | |
| CGGTGAATGTGTGGCGGCGT+GGG | + | chr1.3:61577985-61578004 | MS.gene20856:CDS | 65.0% | |
| GTGCAGCGGCAAGCCATGCA+AGG | + | chr1.3:61578026-61578045 | MS.gene20856:CDS | 65.0% | |
| ! | AGTGCGGAAGAGGCGTGTGC+AGG | + | chr1.3:61577950-61577969 | MS.gene20856:CDS | 65.0% |
| ATGTGTGGCGGCGTGGGACG+AGG | + | chr1.3:61577991-61578010 | MS.gene20856:CDS | 70.0% | |
| GCGGTGAATGTGTGGCGGCG+TGG | + | chr1.3:61577984-61578003 | MS.gene20856:CDS | 70.0% | |
| GGCGGCGTGGGACGAGGTAG+AGG | + | chr1.3:61577997-61578016 | MS.gene20856:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 61577737 | 61578132 | 61577737 | ID=MS.gene20856 |
| chr1.3 | mRNA | 61577737 | 61578132 | 61577737 | ID=MS.gene20856.t1;Parent=MS.gene20856 |
| chr1.3 | exon | 61577737 | 61578132 | 61577737 | ID=MS.gene20856.t1.exon1;Parent=MS.gene20856.t1 |
| chr1.3 | CDS | 61577737 | 61578132 | 61577737 | ID=cds.MS.gene20856.t1;Parent=MS.gene20856.t1 |
| Gene Sequence |
| Protein sequence |
