AlfalfaGEDB | MS.gene24099

M. sativa cultivar XinJiangDaYe / MS.gene24099

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24099.t1	XP_003589562.1	91.7	254	14	2	1	254	1	247	1.60E-128	468.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24099.t1	Q9SGS5	31.6	285	132	9	4	250	13	272	6.3e-20	99.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24099.t1	G7I8D2	91.7	254	14	2	1	254	1	247	1.2e-128	468.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049181	MS.gene24099	0.848013	8.03E-60	-1.69E-46
MS.gene049457	MS.gene24099	0.819245	1.28E-52	-1.69E-46
MS.gene050363	MS.gene24099	0.810876	9.27E-51	-1.69E-46
MS.gene050461	MS.gene24099	0.81493	1.20E-51	-1.69E-46
MS.gene050526	MS.gene24099	0.833653	4.71E-56	-1.69E-46
MS.gene050883	MS.gene24099	0.810211	1.29E-50	-1.69E-46
MS.gene051959	MS.gene24099	0.845646	3.57E-59	-1.69E-46
MS.gene051960	MS.gene24099	0.84485	5.86E-59	-1.69E-46
MS.gene052053	MS.gene24099	0.819671	1.03E-52	-1.69E-46
MS.gene052091	MS.gene24099	0.854053	1.59E-61	-1.69E-46
MS.gene053826	MS.gene24099	0.830057	3.64E-55	-1.69E-46
MS.gene053932	MS.gene24099	0.860311	2.26E-63	-1.69E-46
MS.gene055549	MS.gene24099	0.868294	7.26E-66	-1.69E-46
MS.gene055550	MS.gene24099	0.873254	1.70E-67	-1.69E-46
MS.gene055551	MS.gene24099	0.866604	2.52E-65	-1.69E-46
MS.gene056311	MS.gene24099	0.804412	2.19E-49	-1.69E-46
MS.gene056365	MS.gene24099	0.811051	8.49E-51	-1.69E-46
MS.gene056408	MS.gene24099	0.807718	4.41E-50	-1.69E-46
MS.gene056530	MS.gene24099	0.849678	2.77E-60	-1.69E-46
MS.gene057001	MS.gene24099	0.824388	8.29E-54	-1.69E-46
MS.gene057093	MS.gene24099	0.844983	5.39E-59	-1.69E-46
MS.gene058628	MS.gene24099	0.83613	1.12E-56	-1.69E-46
MS.gene058629	MS.gene24099	0.836277	1.03E-56	-1.69E-46
MS.gene058630	MS.gene24099	0.839319	1.70E-57	-1.69E-46
MS.gene060237	MS.gene24099	0.808484	3.03E-50	-1.69E-46
MS.gene06023	MS.gene24099	0.805271	1.45E-49	-1.69E-46
MS.gene060446	MS.gene24099	0.828082	1.10E-54	-1.69E-46
MS.gene060964	MS.gene24099	0.832407	9.62E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24099.t1	MTR_1g026110	91.732	254	14	2	1	254	1	247	2.43e-163	452
MS.gene24099.t1	MTR_3g114750	48.485	264	98	11	4	250	2	244	3.07e-59	188

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24099.t1	AT1G76070	33.212	274	120	10	15	250	24	272	2.80e-28	108
MS.gene24099.t1	AT1G20310	34.686	271	120	11	1	247	1	238	3.51e-27	105

Find 61 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAGATCTTTGTTGGGTTT+TGG	0.236347	1.2:+198061	None:intergenic
TCCCTTTGATCAATTTCTTC+AGG	0.304197	1.2:+197815	None:intergenic
TTCACCAAAGATTTCTTGTA+TGG	0.307383	1.2:-198116	MS.gene24099:CDS
TCATTGCCACCACCATCTTT+AGG	0.317528	1.2:+198154	None:intergenic
CCTAAAATAGGTGCAGAAAA+TGG	0.326900	1.2:+197755	None:intergenic
TTTCCACCAACACCTAAAAT+AGG	0.335034	1.2:+197743	None:intergenic
CTAAATTTGTGATCTCTTCC+TGG	0.339147	1.2:+198280	None:intergenic
TTTACCTCCGTATCCACCTT+TGG	0.375428	1.2:+198019	None:intergenic
GAAAGGAAATCAGATGGTTC+TGG	0.386986	1.2:-197944	MS.gene24099:CDS
CAAGAAATCTTTGGTGAAGT+AGG	0.393822	1.2:+198121	None:intergenic
CAAGGAATGAGATTAATCTT+TGG	0.407911	1.2:-197682	MS.gene24099:CDS
TTAGGTGTTGGTGGAAATAG+TGG	0.423953	1.2:-197737	MS.gene24099:CDS
TGTCCCATACAAGAAATCTT+TGG	0.426848	1.2:+198112	None:intergenic
GCCTGAAGAAATTGATCAAA+GGG	0.432040	1.2:-197816	MS.gene24099:CDS
GGAAGTGAAGAGATCTTTGT+TGG	0.442034	1.2:+198054	None:intergenic
CAAAGATTAATCTCATTCCT+TGG	0.442885	1.2:+197683	None:intergenic
CAAAACGCTCCTTTCAGTCC+AGG	0.469371	1.2:-198298	MS.gene24099:CDS
AGAAAATAACACAAAGTCAT+GGG	0.481776	1.2:-198254	MS.gene24099:CDS
GGTTCTGGATTTGTTGATGA+AGG	0.482613	1.2:-197929	MS.gene24099:CDS
CACTCCACCAAAGGTGGATA+CGG	0.483600	1.2:-198026	MS.gene24099:CDS
CAAAGGGATTGCTTTACTGA+TGG	0.491176	1.2:-197800	MS.gene24099:CDS
ACCTCCGTATCCACCTTTGG+TGG	0.491667	1.2:+198022	None:intergenic
CACCGCCGATGGGTGATATG+AGG	0.497283	1.2:-197895	MS.gene24099:CDS
GGATTCAACTGAAGAGGCCT+AGG	0.502469	1.2:+197638	None:intergenic
ATTTATTTAGCTTTAAGCAC+AGG	0.503675	1.2:+197617	None:intergenic
AAAACTCATGCTACTGGTAA+GGG	0.508213	1.2:-198226	MS.gene24099:CDS
CGTATCCACCTTTGGTGGAG+TGG	0.514538	1.2:+198027	None:intergenic
CTGATTTGAAGTTTAAGCCA+AGG	0.518893	1.2:-197700	MS.gene24099:CDS
TGATCTCTTCCTGGACTGAA+AGG	0.521071	1.2:+198289	None:intergenic
CAGAAAATAACACAAAGTCA+TGG	0.527453	1.2:-198255	MS.gene24099:CDS
AAGAGAAACAAAGTAAGCTA+AGG	0.530956	1.2:-198354	MS.gene24099:CDS
GCTAGAAGAAAACCTAAAGA+TGG	0.536495	1.2:-198166	MS.gene24099:CDS
GAAGTGAAGAGATCTTTGTT+GGG	0.537217	1.2:+198055	None:intergenic
CACCTTTGGTGGAGTGGACA+CGG	0.538901	1.2:+198033	None:intergenic
AGCCTGAAGAAATTGATCAA+AGG	0.541841	1.2:-197817	MS.gene24099:CDS
TAAAGAGATCGAAGTTTCAG+AGG	0.548970	1.2:-198000	MS.gene24099:CDS
CGTGTCCACTCCACCAAAGG+TGG	0.560517	1.2:-198032	MS.gene24099:CDS
AACAACAAAGCACCGCCGAT+GGG	0.567253	1.2:-197905	MS.gene24099:CDS
AGCACAGGATTCAACTGAAG+AGG	0.568748	1.2:+197632	None:intergenic
TCACCAAAGATTTCTTGTAT+GGG	0.570550	1.2:-198115	MS.gene24099:CDS
TTCCGTGTCCACTCCACCAA+AGG	0.573551	1.2:-198035	MS.gene24099:CDS
GTTCTGGATTTGTTGATGAA+GGG	0.581024	1.2:-197928	MS.gene24099:CDS
AGAAAACCTAAAGATGGTGG+TGG	0.587690	1.2:-198160	MS.gene24099:CDS
TATGGAATCATAGTAACCAT+AGG	0.589627	1.2:+198193	None:intergenic
GAACAACAAAGCACCGCCGA+TGG	0.591209	1.2:-197906	MS.gene24099:CDS
GAGATTTGCAAGTGGACGTG+AGG	0.598149	1.2:-197873	MS.gene24099:CDS
TTTGCGGAAAGGAAATCAGA+TGG	0.598533	1.2:-197950	MS.gene24099:CDS
TTGCTTTACTGATGGAGAGG+AGG	0.603818	1.2:-197792	MS.gene24099:CDS
GGATTGCTTTACTGATGGAG+AGG	0.611817	1.2:-197795	MS.gene24099:CDS
CTAAAGCGAAATTTGCGGAA+AGG	0.612185	1.2:-197961	MS.gene24099:CDS
GAAGAACAATGGCTCCACCT+AGG	0.627085	1.2:-197655	MS.gene24099:CDS
TTCAACTGAAGAGGCCTAGG+TGG	0.634353	1.2:+197641	None:intergenic
AGAAGAAAACCTAAAGATGG+TGG	0.634473	1.2:-198163	MS.gene24099:CDS
TCTTTGGAAGAGAAGAACAA+TGG	0.638654	1.2:-197666	MS.gene24099:CDS
AATCTCCTCATATCACCCAT+CGG	0.643282	1.2:+197890	None:intergenic
GAAAATAACACAAAGTCATG+GGG	0.645047	1.2:-198253	MS.gene24099:CDS
AAATAGTGGTACATCTAATG+TGG	0.671533	1.2:-197723	MS.gene24099:CDS
TCCACCAAAGGTGGATACGG+AGG	0.678054	1.2:-198023	MS.gene24099:CDS
CTCCTCATATCACCCATCGG+CGG	0.680805	1.2:+197893	None:intergenic
GAGGTCTAAAGCGAAATTTG+CGG	0.681767	1.2:-197966	MS.gene24099:CDS
GATATGAGGAGATTTGCAAG+TGG	0.723470	1.2:-197881	MS.gene24099:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTCTAAATTTGATTGGA+AGG	-	chr1.2:198131-198150	MS.gene24099:CDS	20.0%
!	AGAAAATAACACAAAGTCAT+GGG	-	chr1.2:197726-197745	MS.gene24099:CDS	25.0%
!!!	AGGCTTTTTCTAAATTTGAT+TGG	-	chr1.2:198127-198146	MS.gene24099:CDS	25.0%
	AAATAGTGGTACATCTAATG+TGG	-	chr1.2:198257-198276	MS.gene24099:CDS	30.0%
	AAGAGAAACAAAGTAAGCTA+AGG	-	chr1.2:197626-197645	MS.gene24099:CDS	30.0%
	CAAAGATTAATCTCATTCCT+TGG	+	chr1.2:198300-198319	None:intergenic	30.0%
	CAAGGAATGAGATTAATCTT+TGG	-	chr1.2:198298-198317	MS.gene24099:CDS	30.0%
	CAGAAAATAACACAAAGTCA+TGG	-	chr1.2:197725-197744	MS.gene24099:CDS	30.0%
	GAAAATAACACAAAGTCATG+GGG	-	chr1.2:197727-197746	MS.gene24099:CDS	30.0%
	TATGGAATCATAGTAACCAT+AGG	+	chr1.2:197790-197809	None:intergenic	30.0%
	TCACCAAAGATTTCTTGTAT+GGG	-	chr1.2:197865-197884	MS.gene24099:CDS	30.0%
	TTCACCAAAGATTTCTTGTA+TGG	-	chr1.2:197864-197883	MS.gene24099:CDS	30.0%
	AAAAACTCATGCTACTGGTA+AGG	-	chr1.2:197753-197772	MS.gene24099:CDS	35.0%
	AAAACTCATGCTACTGGTAA+GGG	-	chr1.2:197754-197773	MS.gene24099:CDS	35.0%
	AGAAGAAAACCTAAAGATGG+TGG	-	chr1.2:197817-197836	MS.gene24099:CDS	35.0%
	AGCCTGAAGAAATTGATCAA+AGG	-	chr1.2:198163-198182	MS.gene24099:CDS	35.0%
	CAAGAAATCTTTGGTGAAGT+AGG	+	chr1.2:197862-197881	None:intergenic	35.0%
	CCTAAAATAGGTGCAGAAAA+TGG	+	chr1.2:198228-198247	None:intergenic	35.0%
	CTAAATTTGTGATCTCTTCC+TGG	+	chr1.2:197703-197722	None:intergenic	35.0%
	GAAGTGAAGAGATCTTTGTT+GGG	+	chr1.2:197928-197947	None:intergenic	35.0%
	GCCTGAAGAAATTGATCAAA+GGG	-	chr1.2:198164-198183	MS.gene24099:CDS	35.0%
	GCTAGAAGAAAACCTAAAGA+TGG	-	chr1.2:197814-197833	MS.gene24099:CDS	35.0%
	TAAAGAGATCGAAGTTTCAG+AGG	-	chr1.2:197980-197999	MS.gene24099:CDS	35.0%
	TCCCTTTGATCAATTTCTTC+AGG	+	chr1.2:198168-198187	None:intergenic	35.0%
	TGTCCCATACAAGAAATCTT+TGG	+	chr1.2:197871-197890	None:intergenic	35.0%
	TTTCCACCAACACCTAAAAT+AGG	+	chr1.2:198240-198259	None:intergenic	35.0%
!	AAGAGATCTTTGTTGGGTTT+TGG	+	chr1.2:197922-197941	None:intergenic	35.0%
!	CCATTTTCTGCACCTATTTT+AGG	-	chr1.2:198225-198244	MS.gene24099:CDS	35.0%
!	CTGATTTGAAGTTTAAGCCA+AGG	-	chr1.2:198280-198299	MS.gene24099:CDS	35.0%
!	TCTTTGGAAGAGAAGAACAA+TGG	-	chr1.2:198314-198333	MS.gene24099:CDS	35.0%
!!	GTTCTGGATTTGTTGATGAA+GGG	-	chr1.2:198052-198071	MS.gene24099:CDS	35.0%
!!!	GGTTTTCTTCTAGCTTCATA+TGG	+	chr1.2:197808-197827	None:intergenic	35.0%
	AATCTCCTCATATCACCCAT+CGG	+	chr1.2:198093-198112	None:intergenic	40.0%
	CAAAGGGATTGCTTTACTGA+TGG	-	chr1.2:198180-198199	MS.gene24099:CDS	40.0%
	CTAAAGCGAAATTTGCGGAA+AGG	-	chr1.2:198019-198038	MS.gene24099:CDS	40.0%
	GAAAGGAAATCAGATGGTTC+TGG	-	chr1.2:198036-198055	MS.gene24099:CDS	40.0%
	GATATGAGGAGATTTGCAAG+TGG	-	chr1.2:198099-198118	MS.gene24099:CDS	40.0%
	GGAAGTGAAGAGATCTTTGT+TGG	+	chr1.2:197929-197948	None:intergenic	40.0%
	TTTGCGGAAAGGAAATCAGA+TGG	-	chr1.2:198030-198049	MS.gene24099:CDS	40.0%
!	AGAAAACCTAAAGATGGTGG+TGG	-	chr1.2:197820-197839	MS.gene24099:CDS	40.0%
!	GAGGTCTAAAGCGAAATTTG+CGG	-	chr1.2:198014-198033	MS.gene24099:CDS	40.0%
!	TAAGGGATTTTCTGGACCTA+TGG	-	chr1.2:197771-197790	MS.gene24099:CDS	40.0%
!!	GGTTCTGGATTTGTTGATGA+AGG	-	chr1.2:198051-198070	MS.gene24099:CDS	40.0%
!!	TCTGCACCTATTTTAGGTGT+TGG	-	chr1.2:198231-198250	MS.gene24099:CDS	40.0%
!!	TTAGGTGTTGGTGGAAATAG+TGG	-	chr1.2:198243-198262	MS.gene24099:CDS	40.0%
!!!	TTCAGAGGATTTTTTCGCAG+AGG	-	chr1.2:197995-198014	MS.gene24099:CDS	40.0%
	AGCACAGGATTCAACTGAAG+AGG	+	chr1.2:198351-198370	None:intergenic	45.0%
	GGATTGCTTTACTGATGGAG+AGG	-	chr1.2:198185-198204	MS.gene24099:CDS	45.0%
	GGTGCAAAAACTCATGCTAC+TGG	-	chr1.2:197748-197767	MS.gene24099:CDS	45.0%
	TCATTGCCACCACCATCTTT+AGG	+	chr1.2:197829-197848	None:intergenic	45.0%
	TGATCTCTTCCTGGACTGAA+AGG	+	chr1.2:197694-197713	None:intergenic	45.0%
	TTGCTTTACTGATGGAGAGG+AGG	-	chr1.2:198188-198207	MS.gene24099:CDS	45.0%
	TTTACCTCCGTATCCACCTT+TGG	+	chr1.2:197964-197983	None:intergenic	45.0%
!	GCTACTGGTAAGGGATTTTC+TGG	-	chr1.2:197763-197782	MS.gene24099:CDS	45.0%
!!!	GCACCTATTTTAGGTGTTGG+TGG	-	chr1.2:198234-198253	MS.gene24099:CDS	45.0%
	CAAAACGCTCCTTTCAGTCC+AGG	-	chr1.2:197682-197701	MS.gene24099:CDS	50.0%
	CACTCCACCAAAGGTGGATA+CGG	-	chr1.2:197954-197973	MS.gene24099:CDS	50.0%
	GAAGAACAATGGCTCCACCT+AGG	-	chr1.2:198325-198344	MS.gene24099:CDS	50.0%
	GAGATTTGCAAGTGGACGTG+AGG	-	chr1.2:198107-198126	MS.gene24099:CDS	50.0%
	GGATTCAACTGAAGAGGCCT+AGG	+	chr1.2:198345-198364	None:intergenic	50.0%
	TTCAACTGAAGAGGCCTAGG+TGG	+	chr1.2:198342-198361	None:intergenic	50.0%
!!	AACAACAAAGCACCGCCGAT+GGG	-	chr1.2:198075-198094	MS.gene24099:CDS	50.0%
	ACCTCCGTATCCACCTTTGG+TGG	+	chr1.2:197961-197980	None:intergenic	55.0%
	CACCTTTGGTGGAGTGGACA+CGG	+	chr1.2:197950-197969	None:intergenic	55.0%
	CGTATCCACCTTTGGTGGAG+TGG	+	chr1.2:197956-197975	None:intergenic	55.0%
	CTCCTCATATCACCCATCGG+CGG	+	chr1.2:198090-198109	None:intergenic	55.0%
	TCCACCAAAGGTGGATACGG+AGG	-	chr1.2:197957-197976	MS.gene24099:CDS	55.0%
	TTCCGTGTCCACTCCACCAA+AGG	-	chr1.2:197945-197964	MS.gene24099:CDS	55.0%
!!	GAACAACAAAGCACCGCCGA+TGG	-	chr1.2:198074-198093	MS.gene24099:CDS	55.0%
	CACCGCCGATGGGTGATATG+AGG	-	chr1.2:198085-198104	MS.gene24099:CDS	60.0%
	CGTGTCCACTCCACCAAAGG+TGG	-	chr1.2:197948-197967	MS.gene24099:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	197619	198383	197619	ID=MS.gene24099
chr1.2	mRNA	197619	198383	197619	ID=MS.gene24099.t1;Parent=MS.gene24099
chr1.2	exon	197619	198383	197619	ID=MS.gene24099.t1.exon1;Parent=MS.gene24099.t1
chr1.2	CDS	197619	198383	197619	ID=cds.MS.gene24099.t1;Parent=MS.gene24099.t1

Gene Sequence

>MS.gene24099

ATGGAGAAAGAGAAACAAAGTAAGCTAAGGAACAAGTTCATGAAAGCAATCACAATTACATTTCAAAACGCTCCTTTCAGTCCAGGAAGAGATCACAAATTTAGATCAGAAAATAACACAAAGTCATGGGGTGCAAAAACTCATGCTACTGGTAAGGGATTTTCTGGACCTATGGTTACTATGATTCCATATGAAGCTAGAAGAAAACCTAAAGATGGTGGTGGCAATGAAACTCAAGAACCTACTTCACCAAAGATTTCTTGTATGGGACAGATCAAGCATCACAAGAAGAAACATATTCCAAAACCCAACAAAGATCTCTTCACTTCCGTGTCCACTCCACCAAAGGTGGATACGGAGGTAAAGAGATCGAAGTTTCAGAGGATTTTTTCGCAGAGGTCTAAAGCGAAATTTGCGGAAAGGAAATCAGATGGTTCTGGATTTGTTGATGAAGGGAACAACAAAGCACCGCCGATGGGTGATATGAGGAGATTTGCAAGTGGACGTGAGGCTTTTTCTAAATTTGATTGGAAGGCAGTGATTGAGCCTGAAGAAATTGATCAAAGGGATTGCTTTACTGATGGAGAGGAGGATGAAATTTTGATTCCATTTTCTGCACCTATTTTAGGTGTTGGTGGAAATAGTGGTACATCTAATGTGGCTGATTTGAAGTTTAAGCCAAGGAATGAGATTAATCTTTGGAAGAGAAGAACAATGGCTCCACCTAGGCCTCTTCAGTTGAATCCTGTGCTTAAAGCTAAATAA

Protein sequence

>MS.gene24099.t1

MEKEKQSKLRNKFMKAITITFQNAPFSPGRDHKFRSENNTKSWGAKTHATGKGFSGPMVTMIPYEARRKPKDGGGNETQEPTSPKISCMGQIKHHKKKHIPKPNKDLFTSVSTPPKVDTEVKRSKFQRIFSQRSKAKFAERKSDGSGFVDEGNNKAPPMGDMRRFASGREAFSKFDWKAVIEPEEIDQRDCFTDGEEDEILIPFSAPILGVGGNSGTSNVADLKFKPRNEINLWKRRTMAPPRPLQLNPVLKAK