AlfalfaGEDB | MS.gene25757

M. sativa cultivar XinJiangDaYe / MS.gene25757

KEGG: -
GO: -
PFAM: PF03016.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25757.t1	KEH35171.1	95	400	18	1	34	431	29	428	1.80E-227	798.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25757.t1	Q9SSE8	66.3	365	122	1	68	431	87	451	7.9e-147	521.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25757.t1	A0A072V0D6	95.0	400	18	1	34	431	29	428	1.3e-227	798.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049356	MS.gene25757	0.803762	2.99E-49	-1.69E-46
MS.gene049396	MS.gene25757	0.813611	2.34E-51	-1.69E-46
MS.gene050499	MS.gene25757	0.831706	1.43E-55	-1.69E-46
MS.gene050502	MS.gene25757	0.830077	3.60E-55	-1.69E-46
MS.gene050520	MS.gene25757	0.807235	5.58E-50	-1.69E-46
MS.gene051509	MS.gene25757	0.850647	1.48E-60	-1.69E-46
MS.gene053018	MS.gene25757	0.806538	7.83E-50	-1.69E-46
MS.gene053436	MS.gene25757	0.807859	4.11E-50	-1.69E-46
MS.gene053626	MS.gene25757	0.803147	4.00E-49	-1.69E-46
MS.gene053813	MS.gene25757	0.812385	4.35E-51	-1.69E-46
MS.gene054486	MS.gene25757	0.82408	9.79E-54	-1.69E-46
MS.gene054595	MS.gene25757	0.818836	1.59E-52	-1.69E-46
MS.gene055909	MS.gene25757	0.813763	2.17E-51	-1.69E-46
MS.gene055911	MS.gene25757	0.812395	4.33E-51	-1.69E-46
MS.gene056422	MS.gene25757	0.841106	5.81E-58	-1.69E-46
MS.gene057086	MS.gene25757	0.834434	3.00E-56	-1.69E-46
MS.gene05787	MS.gene25757	0.804416	2.18E-49	-1.69E-46
MS.gene058075	MS.gene25757	0.812445	4.23E-51	-1.69E-46
MS.gene058459	MS.gene25757	0.805741	1.15E-49	-1.69E-46
MS.gene059367	MS.gene25757	0.850788	1.35E-60	-1.69E-46
MS.gene059368	MS.gene25757	0.858779	6.52E-63	-1.69E-46
MS.gene059711	MS.gene25757	0.809838	1.55E-50	-1.69E-46
MS.gene060372	MS.gene25757	0.812356	4.42E-51	-1.69E-46
MS.gene060482	MS.gene25757	0.804626	1.97E-49	-1.69E-46
MS.gene06097	MS.gene25757	0.800447	1.44E-48	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene25757	MS.gene027811	PPI
MS.gene25757	MS.gene036708	PPI
MS.gene25757	MS.gene068605	PPI
MS.gene25757	MS.gene069139	PPI
MS.gene25757	MS.gene58027	PPI
MS.gene25757	MS.gene070886	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25757.t1	MTR_3g081240	95.000	400	18	1	34	431	29	428	0.0	801
MS.gene25757.t1	MTR_3g080690	94.975	398	20	0	34	431	29	426	0.0	801
MS.gene25757.t1	MTR_3g097310	62.568	366	135	1	66	431	110	473	1.05e-163	473
MS.gene25757.t1	MTR_1g061760	57.493	367	149	2	66	431	106	466	8.41e-152	442
MS.gene25757.t1	MTR_7g103260	54.076	368	159	2	66	431	20	379	7.78e-146	424
MS.gene25757.t1	MTR_7g103270	52.284	394	169	3	40	431	4	380	5.12e-145	422
MS.gene25757.t1	MTR_3g106690	57.143	322	135	2	113	431	52	373	5.82e-131	386
MS.gene25757.t1	MTR_4g115540	49.867	375	180	3	65	433	236	608	1.91e-126	382
MS.gene25757.t1	MTR_4g115460	46.970	396	200	6	61	450	72	463	1.20e-120	362
MS.gene25757.t1	MTR_4g115530	47.453	373	187	4	65	431	3	372	3.30e-116	348
MS.gene25757.t1	MTR_4g115530	48.428	318	158	2	120	431	1	318	1.33e-101	309
MS.gene25757.t1	MTR_1g006600	44.180	378	193	5	58	431	206	569	2.36e-98	309
MS.gene25757.t1	MTR_4g100730	44.767	344	180	5	95	431	178	518	1.90e-97	305
MS.gene25757.t1	MTR_4g115540	50.166	301	142	3	65	359	236	534	2.09e-97	305
MS.gene25757.t1	MTR_8g102840	41.689	379	202	6	58	431	228	592	7.28e-96	303
MS.gene25757.t1	MTR_7g103150	43.011	372	195	5	64	431	109	467	9.15e-96	299
MS.gene25757.t1	MTR_1g090733	42.700	363	189	7	75	431	181	530	1.92e-94	298
MS.gene25757.t1	MTR_3g110198	44.545	330	176	3	106	431	250	576	1.38e-92	294
MS.gene25757.t1	MTR_3g110200	42.398	342	187	4	94	431	238	573	2.61e-90	288
MS.gene25757.t1	MTR_8g102840	39.286	308	168	6	58	360	228	521	1.20e-66	224
MS.gene25757.t1	MTR_5g031480	34.795	342	207	6	104	439	74	405	2.04e-63	213
MS.gene25757.t1	MTR_5g049790	34.808	339	195	7	104	439	110	425	4.45e-59	201
MS.gene25757.t1	MTR_7g085840	35.168	327	191	9	120	437	1	315	9.47e-49	171
MS.gene25757.t1	MTR_5g031480	33.708	267	161	6	104	364	74	330	7.50e-41	150
MS.gene25757.t1	MTR_5g031480	33.835	266	160	6	104	363	74	329	1.81e-40	149
MS.gene25757.t1	MTR_5g049790	35.211	213	125	5	104	313	127	329	6.29e-31	123
MS.gene25757.t1	MTR_5g049790	35.211	213	125	5	104	313	110	312	7.56e-31	122
MS.gene25757.t1	MTR_8g096140	27.758	281	177	7	151	412	81	354	3.16e-24	105
MS.gene25757.t1	MTR_8g096160	24.359	312	212	6	151	443	81	387	3.28e-24	105
MS.gene25757.t1	MTR_2g084245	27.607	326	193	13	120	414	85	398	8.11e-22	98.6
MS.gene25757.t1	MTR_4g057500	27.607	326	189	13	122	414	105	416	1.68e-21	97.8
MS.gene25757.t1	MTR_3g037720	26.494	385	219	18	121	455	112	482	3.46e-19	90.9
MS.gene25757.t1	MTR_8g080490	25.249	301	208	7	146	434	97	392	1.79e-17	85.1
MS.gene25757.t1	MTR_6g027310	26.804	291	192	9	154	431	174	456	4.95e-17	84.3
MS.gene25757.t1	MTR_4g084200	23.103	290	199	9	161	433	158	440	7.30e-17	83.6
MS.gene25757.t1	MTR_3g085480	25.000	288	191	10	163	434	116	394	3.35e-14	75.1
MS.gene25757.t1	MTR_4g077920	25.503	298	190	11	154	426	98	388	3.87e-14	75.1
MS.gene25757.t1	MTR_4g084200	22.500	280	193	9	161	423	158	430	4.13e-14	74.7
MS.gene25757.t1	MTR_4g084200	22.711	273	187	9	161	416	158	423	1.67e-13	72.8
MS.gene25757.t1	MTR_7g087100	25.000	288	189	10	163	434	188	464	6.04e-13	71.6
MS.gene25757.t1	MTR_1g029350	26.168	321	206	13	155	456	118	426	1.65e-12	69.7
MS.gene25757.t1	MTR_5g081290	30.464	151	87	3	120	270	1	133	2.37e-12	67.8
MS.gene25757.t1	MTR_3g080680	45.333	75	36	2	439	510	126	198	4.40e-12	65.5
MS.gene25757.t1	MTR_1g061790	39.669	121	57	4	47	164	87	194	5.18e-12	65.5
MS.gene25757.t1	MTR_1g097090	24.915	293	183	9	163	425	139	424	1.21e-11	67.4
MS.gene25757.t1	MTR_1g097090	24.915	293	183	9	163	425	139	424	1.41e-11	67.0
MS.gene25757.t1	MTR_4g077920	25.764	229	147	9	215	426	224	446	2.54e-11	66.2
MS.gene25757.t1	MTR_4g077920	25.764	229	147	9	215	426	224	446	3.04e-11	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25757.t1	AT3G07620	66.940	366	118	3	68	431	76	440	0.0	520
MS.gene25757.t1	AT5G03795	56.403	367	154	1	65	431	137	497	9.53e-142	418
MS.gene25757.t1	AT5G25310	49.742	388	183	5	64	447	98	477	3.63e-131	390
MS.gene25757.t1	AT5G11130	44.671	441	216	6	22	450	53	477	7.67e-125	374
MS.gene25757.t1	AT5G20260	46.923	390	199	6	64	450	82	466	6.93e-116	350
MS.gene25757.t1	AT5G11130	49.851	335	162	3	120	450	1	333	1.08e-115	345
MS.gene25757.t1	AT3G42180	47.222	396	198	3	65	451	77	470	5.00e-113	343
MS.gene25757.t1	AT5G33290	47.500	360	178	3	97	447	139	496	1.52e-110	337
MS.gene25757.t1	AT4G16745	44.715	369	187	6	70	431	156	514	3.37e-104	323
MS.gene25757.t1	AT3G42180	46.921	341	172	2	120	451	1	341	7.05e-104	315
MS.gene25757.t1	AT5G20260	47.493	339	175	3	115	450	23	361	1.95e-103	315
MS.gene25757.t1	AT5G20260	47.605	334	172	3	120	450	1	334	3.72e-103	313
MS.gene25757.t1	AT4G16745	44.414	367	187	6	70	429	156	512	4.72e-102	318
MS.gene25757.t1	AT5G19670	43.505	331	179	3	106	431	263	590	7.34e-96	303
MS.gene25757.t1	AT5G19670	43.505	331	179	3	106	431	263	590	7.34e-96	303
MS.gene25757.t1	AT4G32790	43.478	368	190	6	69	431	222	576	1.21e-95	302
MS.gene25757.t1	AT4G32790	43.478	368	190	6	69	431	222	576	1.21e-95	302
MS.gene25757.t1	AT4G32790	43.478	368	190	6	69	431	222	576	1.21e-95	302
MS.gene25757.t1	AT5G37000	37.750	400	227	7	51	432	140	535	5.37e-89	283
MS.gene25757.t1	AT5G25820	42.651	347	186	8	93	431	294	635	1.99e-86	280
MS.gene25757.t1	AT5G11610	39.024	369	206	8	68	431	174	528	1.77e-85	274
MS.gene25757.t1	AT4G38040	34.118	340	208	6	104	437	84	413	1.08e-58	200
MS.gene25757.t1	AT5G11610	37.024	289	164	7	68	352	174	448	2.47e-58	200
MS.gene25757.t1	AT4G38040	33.851	322	197	6	104	419	84	395	8.12e-53	184
MS.gene25757.t1	AT5G11610	36.313	179	102	3	68	244	174	342	1.50e-30	122
MS.gene25757.t1	AT1G27440	26.259	278	183	6	151	411	77	349	1.05e-24	106
MS.gene25757.t1	AT5G61840	25.240	313	202	8	124	412	49	353	3.63e-23	102
MS.gene25757.t1	AT2G28110	25.658	304	198	9	163	443	138	436	9.72e-20	92.4
MS.gene25757.t1	AT1G67410	26.621	293	195	9	157	434	113	400	2.98e-19	90.5
MS.gene25757.t1	AT1G74680	27.336	289	192	7	158	434	144	426	1.44e-18	89.0
MS.gene25757.t1	AT2G35100	24.561	285	182	10	161	426	125	395	3.48e-18	87.4
MS.gene25757.t1	AT3G03650	28.428	299	180	14	158	437	188	471	4.34e-17	84.3
MS.gene25757.t1	AT3G45400	27.931	290	176	10	163	433	154	429	1.35e-16	82.8
MS.gene25757.t1	AT5G22940	24.342	304	192	10	155	431	146	438	3.22e-16	81.6
MS.gene25757.t1	AT5G22940	24.342	304	192	10	155	431	175	467	3.60e-16	81.6
MS.gene25757.t1	AT5G16890	23.675	283	192	10	160	428	182	454	6.86e-14	74.3
MS.gene25757.t1	AT5G44930	26.754	228	133	10	214	422	175	387	4.90e-12	68.6
MS.gene25757.t1	AT5G44930	26.754	228	133	10	214	422	175	387	4.90e-12	68.6
MS.gene25757.t1	AT1G34270	28.814	236	138	10	215	425	195	425	8.45e-12	67.8

Find 104 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTTGCATTGATCTTCTTAT+AGG	0.128865	3.2:-71131007	None:intergenic
GGGAATATTTATCAACTTAT+TGG	0.144366	3.2:+71132177	MS.gene25757:CDS
CCTGTTACCAAGTTGATTTC+AGG	0.167077	3.2:-71132620	None:intergenic
ACCTCAGGTGGTTGGTTTAA+TGG	0.183364	3.2:-71133915	None:intergenic
TAAATTGCCTCAACAATTCT+TGG	0.244588	3.2:-71132869	None:intergenic
GGAAATGATGCATCTTTCTT+TGG	0.250368	3.2:-71132599	None:intergenic
ACAATTCTGGCTTTCTTTGC+AGG	0.251792	3.2:+71132686	MS.gene25757:CDS
TATTCGTGACAAAGCTGTTT+TGG	0.263997	3.2:+71132300	MS.gene25757:CDS
ACAGCTTTGTCACGAATAAC+TGG	0.273001	3.2:-71132295	None:intergenic
TATTGTTGTGATATTATAAT+AGG	0.275605	3.2:-71132905	None:intergenic
TACTCATCTTCATTGTTTCA+TGG	0.291583	3.2:-71132808	None:intergenic
AGGATCATAACAACACTAAA+TGG	0.293875	3.2:-71132256	None:intergenic
CCTGAAATCAACTTGGTAAC+AGG	0.294494	3.2:+71132620	MS.gene25757:CDS
TACAATGGAGAATTGAATTT+AGG	0.295142	3.2:+71130974	MS.gene25757:CDS
TCATGCTTTCTTGTCATGAT+TGG	0.298264	3.2:+71132401	MS.gene25757:CDS
TTGATAATTCAATGAGTAAT+GGG	0.306989	3.2:-71130736	None:intergenic
ATATAGGAGTTACCACTTAA+TGG	0.323102	3.2:+71132069	MS.gene25757:intron
TAGCAGGAAGGATCTTCTTC+TGG	0.323400	3.2:-71133829	None:intergenic
AATGCAAGGAGATAGAAATT+TGG	0.324834	3.2:+71131021	MS.gene25757:CDS
CAACTTTCCAGGCTAAAGTA+TGG	0.325379	3.2:+71133855	MS.gene25757:CDS
CATGCTTTCTTGTCATGATT+GGG	0.337722	3.2:+71132402	MS.gene25757:CDS
TAAATTCAATTCTCCATTGT+AGG	0.358796	3.2:-71130972	None:intergenic
TAGTGAAATTCCCAAGCTTA+AGG	0.365820	3.2:+71132985	MS.gene25757:CDS
TGAAATACATCATATCTCTT+AGG	0.369121	3.2:-71133097	None:intergenic
CAATCAAGAACATCACTAAA+AGG	0.369286	3.2:-71132935	None:intergenic
AAGAAAATTTCCTTAAGCTT+GGG	0.373370	3.2:-71132995	None:intergenic
TCCTGCTATAACAACTTTCC+AGG	0.381638	3.2:+71133844	MS.gene25757:CDS
CAAGAAAATTTCCTTAAGCT+TGG	0.388408	3.2:-71132996	None:intergenic
TATGACTAGTACCAACAGTA+TGG	0.392781	3.2:-71133775	None:intergenic
TATCACACAAATATGCATAT+TGG	0.394028	3.2:+71132353	MS.gene25757:CDS
AAGTAGACATGAGCTTCATT+TGG	0.398848	3.2:-71132229	None:intergenic
TTCACATACCATGTTGCCCT+TGG	0.411752	3.2:-71132515	None:intergenic
AGAAATTTGGAGAAGGTTGA+AGG	0.417058	3.2:+71131034	MS.gene25757:CDS
TAAATAGCTTCTCCATTAAG+TGG	0.424602	3.2:-71132081	None:intergenic
TTTGTACATGTAGGAGGATG+AGG	0.448328	3.2:+71130931	MS.gene25757:intron
AACATGGAACAGAACAATTC+TGG	0.451636	3.2:+71132673	MS.gene25757:CDS
TATCTTTGTTCTTCCAATGT+TGG	0.453246	3.2:-71132742	None:intergenic
TTTGATAATTCAATGAGTAA+TGG	0.455088	3.2:-71130737	None:intergenic
CATGACAAGAAAGCATGAAA+TGG	0.462671	3.2:-71132396	None:intergenic
GCCTGGAAAGTTGTTATAGC+AGG	0.463018	3.2:-71133845	None:intergenic
CTTGCCACTTCCCACCCACT+TGG	0.464582	3.2:-71132845	None:intergenic
TTGCAAGAATATATATTCAA+TGG	0.476651	3.2:+71132153	MS.gene25757:CDS
GCATATTGGAATAGAAGTTA+TGG	0.477539	3.2:+71132367	MS.gene25757:CDS
GACCGTCCCATACTTTAGCC+TGG	0.479999	3.2:-71133862	None:intergenic
AAGAGGCAGTACGGTGTCAT+TGG	0.482673	3.2:-71131104	None:intergenic
TTGGTAACAGGAGAAACAAC+AGG	0.491020	3.2:+71132632	MS.gene25757:CDS
CTTGAAAGGTAAGTAATACA+TGG	0.496221	3.2:-71133801	None:intergenic
GGAGAAACAACAGGTTTACT+TGG	0.502236	3.2:+71132641	MS.gene25757:CDS
TTGTACATGTAGGAGGATGA+GGG	0.505853	3.2:+71130932	MS.gene25757:intron
ATGGCACACTCTCTTACATC+AGG	0.507892	3.2:-71133896	None:intergenic
TTGTCCAAGTGGGTGGGAAG+TGG	0.510845	3.2:+71132841	MS.gene25757:CDS
ATTGTATTTGTCCAAGTGGG+TGG	0.518103	3.2:+71132834	MS.gene25757:CDS
ATACACCTTGTATGATGAAA+GGG	0.521451	3.2:-71130787	None:intergenic
AGGTCATGAAACACAAAAGT+TGG	0.523989	3.2:-71133740	None:intergenic
ACTCTCTGACCTCAGGTGGT+TGG	0.529355	3.2:-71133923	None:intergenic
TATACACCTTGTATGATGAA+AGG	0.534728	3.2:-71130788	None:intergenic
AGAATTGTTCTGTTCCATGT+TGG	0.536389	3.2:-71132671	None:intergenic
GTCAATTGTACCCATACTGT+TGG	0.538189	3.2:+71133764	MS.gene25757:CDS
GAAATATATAAGATTGCAAG+AGG	0.540677	3.2:+71133033	MS.gene25757:CDS
ATTCAATGAGTAATGGGGTG+TGG	0.547831	3.2:-71130730	None:intergenic
ATTACTCATTGAATTATCAA+AGG	0.556943	3.2:+71130739	MS.gene25757:CDS
ATCATTTCCTGAAATCAACT+TGG	0.557019	3.2:+71132613	MS.gene25757:CDS
CTGGGTGAAAGAGATGTTCA+AGG	0.557117	3.2:-71132276	None:intergenic
AGGAGATAGAAATTTGGAGA+AGG	0.557354	3.2:+71131027	MS.gene25757:CDS
AATACATCATATCTCTTAGG+AGG	0.557887	3.2:-71133094	None:intergenic
TTCCAATGTTGGAAAAGCAA+TGG	0.557955	3.2:-71132731	None:intergenic
ATCTACCCTTTCATCATACA+AGG	0.560213	3.2:+71130782	MS.gene25757:CDS
GAAGGAAATGGATGCTGCAA+TGG	0.560884	3.2:+71133358	MS.gene25757:intron
TACTTGGTGGCTATCCAACA+TGG	0.562509	3.2:+71132657	MS.gene25757:CDS
GAAACAACAGGTTTACTTGG+TGG	0.562533	3.2:+71132644	MS.gene25757:CDS
ATTGAATATATATTCTTGCA+AGG	0.562749	3.2:-71132151	None:intergenic
AACTTTCCAGGCTAAAGTAT+GGG	0.564439	3.2:+71133856	MS.gene25757:CDS
CAAGAATATATATTCAATGG+AGG	0.575833	3.2:+71132156	MS.gene25757:CDS
TATAAGACAATGGCCTACAA+TGG	0.581787	3.2:+71130959	MS.gene25757:CDS
GGTGTCATTGGTTCTCAACA+AGG	0.582461	3.2:-71131092	None:intergenic
ATCCTACCTATGAAAAGCAA+AGG	0.584468	3.2:-71131173	None:intergenic
AGAGTCCTCAAGAGGCAGTA+CGG	0.587025	3.2:-71131113	None:intergenic
TTGTATTTGTCCAAGTGGGT+GGG	0.588230	3.2:+71132835	MS.gene25757:CDS
AATATTGTATTTGTCCAAGT+GGG	0.593695	3.2:+71132831	MS.gene25757:CDS
AATTTGCAATGCAAATATCT+CGG	0.599942	3.2:+71132565	MS.gene25757:CDS
TTCTTTGCAGGACAAATGCA+TGG	0.604295	3.2:+71132698	MS.gene25757:CDS
AAGAATATATATTCAATGGA+GGG	0.605925	3.2:+71132157	MS.gene25757:CDS
ATGACTAGTACCAACAGTAT+GGG	0.610332	3.2:-71133774	None:intergenic
GGCAAGTCCAAGAATTGTTG+AGG	0.611209	3.2:+71132862	MS.gene25757:CDS
TAATCATGAGAGTCCTCAAG+AGG	0.613023	3.2:-71131121	None:intergenic
GGGTGTGGATGCATACATCT+TGG	0.614821	3.2:-71130715	None:intergenic
TTTCTGTAGATGTCTCCTTG+TGG	0.615421	3.2:-71131148	None:intergenic
AAATATTGTATTTGTCCAAG+TGG	0.615905	3.2:+71132830	MS.gene25757:CDS
GCACACTCTCTTACATCAGG+TGG	0.617930	3.2:-71133893	None:intergenic
CAGCTTTGTCACGAATAACT+GGG	0.619899	3.2:-71132294	None:intergenic
TATAGGGACCAAGGGCAACA+TGG	0.622985	3.2:+71132507	MS.gene25757:intron
TGACACCGTACTGCCTCTTG+AGG	0.624877	3.2:+71131108	MS.gene25757:CDS
GCTAAAGTATGGGACGGTCA+AGG	0.629484	3.2:+71133866	MS.gene25757:CDS
AAGGGCAACATGGTATGTGA+AGG	0.631000	3.2:+71132517	MS.gene25757:CDS
TGATAATTCAATGAGTAATG+GGG	0.632019	3.2:-71130735	None:intergenic
GCACACTCTCTGACCTCAGG+TGG	0.633417	3.2:-71133927	None:intergenic
GCCATTAAACCAACCACCTG+AGG	0.634243	3.2:+71133914	MS.gene25757:CDS
CCTATAAGAAGATCAATGCA+AGG	0.636919	3.2:+71131007	MS.gene25757:CDS
TTCCAGGCTAAAGTATGGGA+CGG	0.638739	3.2:+71133860	MS.gene25757:CDS
GGAAAGTTGTTATAGCAGGA+AGG	0.640337	3.2:-71133841	None:intergenic
CAGTATGGGTACAATTGACA+AGG	0.650569	3.2:-71133760	None:intergenic
TGAGGGAAGATATAAGACAA+TGG	0.680989	3.2:+71130949	MS.gene25757:CDS
AAATATATAAGATTGCAAGA+GGG	0.689156	3.2:+71133034	MS.gene25757:CDS
TCTCATGATTATGTTCCACA+AGG	0.699811	3.2:+71131133	MS.gene25757:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTATATTTCATGAAAA+TGG	+	chr3.2:71132477-71132496	MS.gene25757:intron	10.0%
!!	ATATGATTATTCATTCTATA+TGG	+	chr3.2:71133119-71133138	MS.gene25757:intron	15.0%
!!	TATTGTTGTGATATTATAAT+AGG	-	chr3.2:71132908-71132927	None:intergenic	15.0%
!!	TGTTCATCTTAATTATATTA+GGG	+	chr3.2:71133716-71133735	MS.gene25757:intron	15.0%
!!	TTGTTCATCTTAATTATATT+AGG	+	chr3.2:71133715-71133734	MS.gene25757:intron	15.0%
!!!	ATTCATTTATTTGTTAATGT+GGG	+	chr3.2:71133200-71133219	MS.gene25757:intron	15.0%
!!!	TATTCATTTATTTGTTAATG+TGG	+	chr3.2:71133199-71133218	MS.gene25757:intron	15.0%
!!!	TTTTTACATAATGATGATAT+AGG	+	chr3.2:71132053-71132072	MS.gene25757:intron	15.0%
!!!	TTTTTTTGAACAATCAAAAA+TGG	+	chr3.2:71131394-71131413	MS.gene25757:intron	15.0%
!!	AAATATATAAGATTGCAAGA+GGG	+	chr3.2:71133034-71133053	MS.gene25757:CDS	20.0%
!!	AAGAATATATATTCAATGGA+GGG	+	chr3.2:71132157-71132176	MS.gene25757:CDS	20.0%
!!	AGTTACAACATTATTGAATA+TGG	+	chr3.2:71131645-71131664	MS.gene25757:intron	20.0%
!!	ATGTATAAGAAGACAATTAT+TGG	-	chr3.2:71130840-71130859	None:intergenic	20.0%
!!	ATTAAGATGAACAATTAGTT+TGG	-	chr3.2:71133709-71133728	None:intergenic	20.0%
!!	ATTACTCATTGAATTATCAA+AGG	+	chr3.2:71130739-71130758	MS.gene25757:CDS	20.0%
!!	ATTGAATATATATTCTTGCA+AGG	-	chr3.2:71132154-71132173	None:intergenic	20.0%
!!	GATTCAAATTCATTTAACAT+AGG	+	chr3.2:71131705-71131724	MS.gene25757:intron	20.0%
!!	TTAAAAATAGTTTCTTAGCT+TGG	+	chr3.2:71131798-71131817	MS.gene25757:intron	20.0%
!!	TTCGTTAAGAATTGATTATA+TGG	-	chr3.2:71133330-71133349	None:intergenic	20.0%
!!	TTGCAAGAATATATATTCAA+TGG	+	chr3.2:71132153-71132172	MS.gene25757:CDS	20.0%
!!!	AAAATTTTTGTGTATGAAGA+GGG	+	chr3.2:71132103-71132122	MS.gene25757:CDS	20.0%
!!!	TAAAATTTTTGTGTATGAAG+AGG	+	chr3.2:71132102-71132121	MS.gene25757:CDS	20.0%
!!!	TTGATAATTCAATGAGTAAT+GGG	-	chr3.2:71130739-71130758	None:intergenic	20.0%
!!!	TTTGATAATTCAATGAGTAA+TGG	-	chr3.2:71130740-71130759	None:intergenic	20.0%
!	AAATATTGTATTTGTCCAAG+TGG	+	chr3.2:71132830-71132849	MS.gene25757:CDS	25.0%
!	AACATTAATTACCATTAGCT+AGG	-	chr3.2:71130884-71130903	None:intergenic	25.0%
!	AACATTATTGAATATGGAGT+TGG	+	chr3.2:71131651-71131670	MS.gene25757:intron	25.0%
!	AAGAAAATTTCCTTAAGCTT+GGG	-	chr3.2:71132998-71133017	None:intergenic	25.0%
!	AATATTGTATTTGTCCAAGT+GGG	+	chr3.2:71132831-71132850	MS.gene25757:CDS	25.0%
!	AATTTGCAATGCAAATATCT+CGG	+	chr3.2:71132565-71132584	MS.gene25757:CDS	25.0%
!	ACAAATGTGAGAAAGATAAA+TGG	+	chr3.2:71133238-71133257	MS.gene25757:intron	25.0%
!	ATATTCATTCTCTTCTGTTT+AGG	-	chr3.2:71133665-71133684	None:intergenic	25.0%
!	ATCTCAACCACATATTAAAT+AGG	-	chr3.2:71131937-71131956	None:intergenic	25.0%
!	ATTGAAGCCTATTTAATATG+TGG	+	chr3.2:71131927-71131946	MS.gene25757:intron	25.0%
!	CAAGAATATATATTCAATGG+AGG	+	chr3.2:71132156-71132175	MS.gene25757:CDS	25.0%
!	CAATCCAAAAAATAATGATC+AGG	+	chr3.2:71133633-71133652	MS.gene25757:intron	25.0%
!	CATACTCACACTATAATTAA+CGG	-	chr3.2:71132449-71132468	None:intergenic	25.0%
!	GAAATATATAAGATTGCAAG+AGG	+	chr3.2:71133033-71133052	MS.gene25757:CDS	25.0%
!	GAGGTTAAAACTCTTAAATA+TGG	-	chr3.2:71131964-71131983	None:intergenic	25.0%
!	GGGAATATTTATCAACTTAT+TGG	+	chr3.2:71132177-71132196	MS.gene25757:CDS	25.0%
!	TAAATTCAATTCTCCATTGT+AGG	-	chr3.2:71130975-71130994	None:intergenic	25.0%
!	TACAATGGAGAATTGAATTT+AGG	+	chr3.2:71130974-71130993	MS.gene25757:CDS	25.0%
!	TATCACACAAATATGCATAT+TGG	+	chr3.2:71132353-71132372	MS.gene25757:CDS	25.0%
!	TCCTTCAAAAAAAATGTAAG+AGG	-	chr3.2:71131983-71132002	None:intergenic	25.0%
!	TGAAATACATCATATCTCTT+AGG	-	chr3.2:71133100-71133119	None:intergenic	25.0%
!	TGAGAACACAAATGTTAAAT+GGG	-	chr3.2:71131830-71131849	None:intergenic	25.0%
!	TGATAATTCAATGAGTAATG+GGG	-	chr3.2:71130738-71130757	None:intergenic	25.0%
!	TTAAATATATGTCTTTAGCC+TGG	-	chr3.2:71131600-71131619	None:intergenic	25.0%
!	TTAACTTTATCTAACAACCT+GGG	+	chr3.2:71131616-71131635	MS.gene25757:intron	25.0%
!	TTATTCATTCTATATGGCTT+AGG	+	chr3.2:71133125-71133144	MS.gene25757:intron	25.0%
!!	AAGCTTAAGGAAATTTTCTT+GGG	+	chr3.2:71132998-71133017	MS.gene25757:CDS	25.0%
!!	TTTAACTTTATCTAACAACC+TGG	+	chr3.2:71131615-71131634	MS.gene25757:intron	25.0%
!!	TTTTAGTGATGTTCTTGATT+GGG	+	chr3.2:71132937-71132956	MS.gene25757:CDS	25.0%
!!!	ACCTCTTACATTTTTTTTGA+AGG	+	chr3.2:71131979-71131998	MS.gene25757:intron	25.0%
!!!	ATGAAAATGGTTTTGTGTAT+AGG	+	chr3.2:71132490-71132509	MS.gene25757:intron	25.0%
!!!	CTCTTAATTTTGATTCATTC+TGG	+	chr3.2:71133471-71133490	MS.gene25757:intron	25.0%
!!!	TGAAAATGGTTTTGTGTATA+GGG	+	chr3.2:71132491-71132510	MS.gene25757:intron	25.0%
!!!	TGAACCTGATCATTATTTTT+TGG	-	chr3.2:71133640-71133659	None:intergenic	25.0%
	AAAGATAGCTCAAAAGATGA+AGG	-	chr3.2:71131898-71131917	None:intergenic	30.0%
	AAATCCACCTAATTGATATC+AGG	+	chr3.2:71131314-71131333	MS.gene25757:intron	30.0%
	AATACATCATATCTCTTAGG+AGG	-	chr3.2:71133097-71133116	None:intergenic	30.0%
	AATGCAAGGAGATAGAAATT+TGG	+	chr3.2:71131021-71131040	MS.gene25757:CDS	30.0%
	AGGATCATAACAACACTAAA+TGG	-	chr3.2:71132259-71132278	None:intergenic	30.0%
	ATACACCTTGTATGATGAAA+GGG	-	chr3.2:71130790-71130809	None:intergenic	30.0%
	ATATAGGAGTTACCACTTAA+TGG	+	chr3.2:71132069-71132088	MS.gene25757:intron	30.0%
	ATCATTTCCTGAAATCAACT+TGG	+	chr3.2:71132613-71132632	MS.gene25757:CDS	30.0%
	CAAGAAAATTTCCTTAAGCT+TGG	-	chr3.2:71132999-71133018	None:intergenic	30.0%
	CAATCAAGAACATCACTAAA+AGG	-	chr3.2:71132938-71132957	None:intergenic	30.0%
	CAATTCTTAACGAATTCTTC+CGG	+	chr3.2:71133336-71133355	MS.gene25757:intron	30.0%
	CTTGAAAGGTAAGTAATACA+TGG	-	chr3.2:71133804-71133823	None:intergenic	30.0%
	GAACTATATCATGAGAGATA+AGG	+	chr3.2:71131201-71131220	MS.gene25757:intron	30.0%
	GCATATTGGAATAGAAGTTA+TGG	+	chr3.2:71132367-71132386	MS.gene25757:CDS	30.0%
	GTATTAGAGTTTACGTTCAA+AGG	+	chr3.2:71131363-71131382	MS.gene25757:intron	30.0%
	GTGAGAACACAAATGTTAAA+TGG	-	chr3.2:71131831-71131850	None:intergenic	30.0%
	TAAATAGCTTCTCCATTAAG+TGG	-	chr3.2:71132084-71132103	None:intergenic	30.0%
	TAAATTGCCTCAACAATTCT+TGG	-	chr3.2:71132872-71132891	None:intergenic	30.0%
	TAATACAACTTCGATATTGC+AGG	-	chr3.2:71131349-71131368	None:intergenic	30.0%
	TACTCATCTTCATTGTTTCA+TGG	-	chr3.2:71132811-71132830	None:intergenic	30.0%
	TATACACCTTGTATGATGAA+AGG	-	chr3.2:71130791-71130810	None:intergenic	30.0%
	TATCTTTGTTCTTCCAATGT+TGG	-	chr3.2:71132745-71132764	None:intergenic	30.0%
!	ACTTTGCTATGTTACTTAAG+TGG	-	chr3.2:71131266-71131285	None:intergenic	30.0%
!	CAAGCTTAAGGAAATTTTCT+TGG	+	chr3.2:71132997-71133016	MS.gene25757:CDS	30.0%
!	CATCTTTTGAGCTATCTTTT+GGG	+	chr3.2:71131899-71131918	MS.gene25757:intron	30.0%
!	CTTTTAGTGATGTTCTTGAT+TGG	+	chr3.2:71132936-71132955	MS.gene25757:CDS	30.0%
!	TCATCTTTTGAGCTATCTTT+TGG	+	chr3.2:71131898-71131917	MS.gene25757:intron	30.0%
!	TCATGGTATGAAATTTTGTC+TGG	-	chr3.2:71132794-71132813	None:intergenic	30.0%
!	TGTTCTTACGGTAATTCTTT+TGG	-	chr3.2:71131465-71131484	None:intergenic	30.0%
!!!	GTTGTTGATTTTGTACATGT+AGG	+	chr3.2:71130922-71130941	MS.gene25757:intron	30.0%
	AAATCTAGTCGTGCAAATGA+TGG	-	chr3.2:71133529-71133548	None:intergenic	35.0%
	AACATGGAACAGAACAATTC+TGG	+	chr3.2:71132673-71132692	MS.gene25757:CDS	35.0%
	AACTTTCCAGGCTAAAGTAT+GGG	+	chr3.2:71133856-71133875	MS.gene25757:CDS	35.0%
	AAGTAGACATGAGCTTCATT+TGG	-	chr3.2:71132232-71132251	None:intergenic	35.0%
	AATAGAGCATGACATGTAAG+AGG	-	chr3.2:71132014-71132033	None:intergenic	35.0%
	AGAAATTTGGAGAAGGTTGA+AGG	+	chr3.2:71131034-71131053	MS.gene25757:CDS	35.0%
	AGAATTGTTCTGTTCCATGT+TGG	-	chr3.2:71132674-71132693	None:intergenic	35.0%
	AGGAGATAGAAATTTGGAGA+AGG	+	chr3.2:71131027-71131046	MS.gene25757:CDS	35.0%
	AGGTCATGAAACACAAAAGT+TGG	-	chr3.2:71133743-71133762	None:intergenic	35.0%
	ATAGATAGATGCGTACACTT+AGG	-	chr3.2:71133409-71133428	None:intergenic	35.0%
	ATCCTACCTATGAAAAGCAA+AGG	-	chr3.2:71131176-71131195	None:intergenic	35.0%
	ATCTACCCTTTCATCATACA+AGG	+	chr3.2:71130782-71130801	MS.gene25757:CDS	35.0%
	ATGACTAGTACCAACAGTAT+GGG	-	chr3.2:71133777-71133796	None:intergenic	35.0%
	ATGTTGTAACTTCTATTCCC+AGG	-	chr3.2:71131636-71131655	None:intergenic	35.0%
	CATGCTTTCTTGTCATGATT+GGG	+	chr3.2:71132402-71132421	MS.gene25757:CDS	35.0%
	CCTATAAGAAGATCAATGCA+AGG	+	chr3.2:71131007-71131026	MS.gene25757:CDS	35.0%
	CGATTCTCCTGATATCAATT+AGG	-	chr3.2:71131324-71131343	None:intergenic	35.0%
	GAGAATAAGGTGATAAGCAT+CGG	-	chr3.2:71133581-71133600	None:intergenic	35.0%
	GATTGTAAACAATCAACGCA+TGG	-	chr3.2:71133618-71133637	None:intergenic	35.0%
	GGAAATGATGCATCTTTCTT+TGG	-	chr3.2:71132602-71132621	None:intergenic	35.0%
	TAAAGTTAGCTCACAAGATG+AGG	-	chr3.2:71131681-71131700	None:intergenic	35.0%
	TAGTGAAATTCCCAAGCTTA+AGG	+	chr3.2:71132985-71133004	MS.gene25757:CDS	35.0%
	TATAAGACAATGGCCTACAA+TGG	+	chr3.2:71130959-71130978	MS.gene25757:CDS	35.0%
	TATGACTAGTACCAACAGTA+TGG	-	chr3.2:71133778-71133797	None:intergenic	35.0%
	TCATGCTTTCTTGTCATGAT+TGG	+	chr3.2:71132401-71132420	MS.gene25757:CDS	35.0%
	TCATGTATGACGTGAAACTT+GGG	-	chr3.2:71131769-71131788	None:intergenic	35.0%
	TCTCATGATTATGTTCCACA+AGG	+	chr3.2:71131133-71131152	MS.gene25757:CDS	35.0%
	TGAGGGAAGATATAAGACAA+TGG	+	chr3.2:71130949-71130968	MS.gene25757:CDS	35.0%
	TTCATGTATGACGTGAAACT+TGG	-	chr3.2:71131770-71131789	None:intergenic	35.0%
	TTCATTCTATATGGCTTAGG+AGG	+	chr3.2:71133128-71133147	MS.gene25757:intron	35.0%
	TTCCAATGTTGGAAAAGCAA+TGG	-	chr3.2:71132734-71132753	None:intergenic	35.0%
	TTCTCCTGATATCAATTAGG+TGG	-	chr3.2:71131321-71131340	None:intergenic	35.0%
!	AATTTTCTTGGGTATCTCTG+AGG	+	chr3.2:71133009-71133028	MS.gene25757:CDS	35.0%
!	CATGACAAGAAAGCATGAAA+TGG	-	chr3.2:71132399-71132418	None:intergenic	35.0%
!	CCTTGCATTGATCTTCTTAT+AGG	-	chr3.2:71131010-71131029	None:intergenic	35.0%
!	TATTCGTGACAAAGCTGTTT+TGG	+	chr3.2:71132300-71132319	MS.gene25757:CDS	35.0%
!	TGACTGACTCTTTGTTCTTA+CGG	-	chr3.2:71131477-71131496	None:intergenic	35.0%
!!	ATTCGTGACAAAGCTGTTTT+GGG	+	chr3.2:71132301-71132320	MS.gene25757:CDS	35.0%
!!	CTCAGAGGTTTTTGAGAATA+AGG	-	chr3.2:71133594-71133613	None:intergenic	35.0%
!!	GAAATGCCTTTGCTTTTCAT+AGG	+	chr3.2:71131167-71131186	MS.gene25757:CDS	35.0%
!!	GTTGATTTTGTACATGTAGG+AGG	+	chr3.2:71130925-71130944	MS.gene25757:intron	35.0%
!!!	TTCTTCTGGTTTTGCTTGAA+AGG	-	chr3.2:71133818-71133837	None:intergenic	35.0%
	AGCTCAATCACTTGGTTATG+AGG	-	chr3.2:71131861-71131880	None:intergenic	40.0%
	ATTGTATTTGTCCAAGTGGG+TGG	+	chr3.2:71132834-71132853	MS.gene25757:CDS	40.0%
	CAACTTTCCAGGCTAAAGTA+TGG	+	chr3.2:71133855-71133874	MS.gene25757:CDS	40.0%
	CAAGGACCATAGTGAAAAAG+AGG	-	chr3.2:71132136-71132155	None:intergenic	40.0%
	CAGTATGGGTACAATTGACA+AGG	-	chr3.2:71133763-71133782	None:intergenic	40.0%
	GAAACAACAGGTTTACTTGG+TGG	+	chr3.2:71132644-71132663	MS.gene25757:CDS	40.0%
	GGAAAGTTGTTATAGCAGGA+AGG	-	chr3.2:71133844-71133863	None:intergenic	40.0%
	GGAGAAACAACAGGTTTACT+TGG	+	chr3.2:71132641-71132660	MS.gene25757:CDS	40.0%
	GTCAATTGTACCCATACTGT+TGG	+	chr3.2:71133764-71133783	MS.gene25757:CDS	40.0%
	TAATCATGAGAGTCCTCAAG+AGG	-	chr3.2:71131124-71131143	None:intergenic	40.0%
	TCCTGCTATAACAACTTTCC+AGG	+	chr3.2:71133844-71133863	MS.gene25757:CDS	40.0%
	TCTTAACGAATTCTTCCGGA+AGG	+	chr3.2:71133340-71133359	MS.gene25757:intron	40.0%
	TTCTTTGCAGGACAAATGCA+TGG	+	chr3.2:71132698-71132717	MS.gene25757:CDS	40.0%
	TTGTACATGTAGGAGGATGA+GGG	+	chr3.2:71130932-71130951	MS.gene25757:intron	40.0%
	TTGTATTTGTCCAAGTGGGT+GGG	+	chr3.2:71132835-71132854	MS.gene25757:CDS	40.0%
	TTTCTGTAGATGTCTCCTTG+TGG	-	chr3.2:71131151-71131170	None:intergenic	40.0%
	TTTGCACGACTAGATTTAGC+TGG	+	chr3.2:71133532-71133551	MS.gene25757:intron	40.0%
	TTTGTACATGTAGGAGGATG+AGG	+	chr3.2:71130931-71130950	MS.gene25757:intron	40.0%
!	AAAAAGGCTGCCTTTAGGTT+TGG	-	chr3.2:71131568-71131587	None:intergenic	40.0%
!	AAACCTAAAGGCAGCCTTTT+TGG	+	chr3.2:71131567-71131586	MS.gene25757:intron	40.0%
!	ACAGCTTTGTCACGAATAAC+TGG	-	chr3.2:71132298-71132317	None:intergenic	40.0%
!	ATTCAATGAGTAATGGGGTG+TGG	-	chr3.2:71130733-71130752	None:intergenic	40.0%
!	CAGCTTTGTCACGAATAACT+GGG	-	chr3.2:71132297-71132316	None:intergenic	40.0%
!	CCTGAAATCAACTTGGTAAC+AGG	+	chr3.2:71132620-71132639	MS.gene25757:CDS	40.0%
!	GTTTTGTGTATAGGGACCAA+GGG	+	chr3.2:71132499-71132518	MS.gene25757:intron	40.0%
!	TTGGTAACAGGAGAAACAAC+AGG	+	chr3.2:71132632-71132651	MS.gene25757:CDS	40.0%
!	TTTTGTGCACACCTAGCTAA+TGG	+	chr3.2:71130870-71130889	MS.gene25757:intron	40.0%
!!	ACAATTCTGGCTTTCTTTGC+AGG	+	chr3.2:71132686-71132705	MS.gene25757:CDS	40.0%
!!	ATAAGCATCGGATGTGTGAA+AGG	-	chr3.2:71133569-71133588	None:intergenic	40.0%
!!	CCTGTTACCAAGTTGATTTC+AGG	-	chr3.2:71132623-71132642	None:intergenic	40.0%
!!	GAACCTCCTCTTTTTCACTA+TGG	+	chr3.2:71132127-71132146	MS.gene25757:CDS	40.0%
!!	GACCATTGCTTTTCCAACAT+TGG	+	chr3.2:71132729-71132748	MS.gene25757:CDS	40.0%
!!	TAAGCATCGGATGTGTGAAA+GGG	-	chr3.2:71133568-71133587	None:intergenic	40.0%
!!	TGCCTTTGCTTTTCATAGGT+AGG	+	chr3.2:71131171-71131190	MS.gene25757:intron	40.0%
!!!	GCTAGATGAGTTGCTTTTTG+TGG	-	chr3.2:71131432-71131451	None:intergenic	40.0%
!!!	GCTGTTTTGGGAAGAACTAT+TGG	+	chr3.2:71132313-71132332	MS.gene25757:CDS	40.0%
	AAGCCAAAAAGGCTGCCTTT+AGG	-	chr3.2:71131573-71131592	None:intergenic	45.0%
	AAGGGCAACATGGTATGTGA+AGG	+	chr3.2:71132517-71132536	MS.gene25757:CDS	45.0%
	ACCAAGTGATTGAGCTCAAC+TGG	+	chr3.2:71131865-71131884	MS.gene25757:intron	45.0%
	ACCAGTTGAGCTCAATCACT+TGG	-	chr3.2:71131869-71131888	None:intergenic	45.0%
	AGCCTGGTTGAAAGCCAAAA+AGG	-	chr3.2:71131584-71131603	None:intergenic	45.0%
	ATGGCACACTCTCTTACATC+AGG	-	chr3.2:71133899-71133918	None:intergenic	45.0%
	CCTATCTGTGCCAAACCTAA+AGG	+	chr3.2:71131555-71131574	MS.gene25757:intron	45.0%
	CGAATTCTTCCGGAAGGAAA+TGG	+	chr3.2:71133346-71133365	MS.gene25757:intron	45.0%
	CTGGGTGAAAGAGATGTTCA+AGG	-	chr3.2:71132279-71132298	None:intergenic	45.0%
	GAAGGAAATGGATGCTGCAA+TGG	+	chr3.2:71133358-71133377	MS.gene25757:intron	45.0%
	GCCTGGAAAGTTGTTATAGC+AGG	-	chr3.2:71133848-71133867	None:intergenic	45.0%
	GGACCATAGTGAAAAAGAGG+AGG	-	chr3.2:71132133-71132152	None:intergenic	45.0%
	GGCAAGTCCAAGAATTGTTG+AGG	+	chr3.2:71132862-71132881	MS.gene25757:CDS	45.0%
	TAGCAGGAAGGATCTTCTTC+TGG	-	chr3.2:71133832-71133851	None:intergenic	45.0%
	TCAAGCCCGTTTCAATGCTT+GGG	-	chr3.2:71131529-71131548	None:intergenic	45.0%
	TTAGGTTTGGCACAGATAGG+TGG	-	chr3.2:71131555-71131574	None:intergenic	45.0%
	TTCACATACCATGTTGCCCT+TGG	-	chr3.2:71132518-71132537	None:intergenic	45.0%
	TTCCAGGCTAAAGTATGGGA+CGG	+	chr3.2:71133860-71133879	MS.gene25757:CDS	45.0%
	TTGCAGCATCCATTTCCTTC+CGG	-	chr3.2:71133358-71133377	None:intergenic	45.0%
!	AATGCTTGGGCATCGCTTAA+TGG	-	chr3.2:71131516-71131535	None:intergenic	45.0%
!	CCTTTAGGTTTGGCACAGAT+AGG	-	chr3.2:71131558-71131577	None:intergenic	45.0%
!	TACTTGGTGGCTATCCAACA+TGG	+	chr3.2:71132657-71132676	MS.gene25757:CDS	45.0%
!!	AGTCGTGCAAATGATGGACA+GGG	-	chr3.2:71133523-71133542	None:intergenic	45.0%
!!	GGTGTCATTGGTTCTCAACA+AGG	-	chr3.2:71131095-71131114	None:intergenic	45.0%
!!	GGTTTTGTGTATAGGGACCA+AGG	+	chr3.2:71132498-71132517	MS.gene25757:intron	45.0%
!!	TAGTCGTGCAAATGATGGAC+AGG	-	chr3.2:71133524-71133543	None:intergenic	45.0%
!!!	AGCCTTTTTGGCTTTCAACC+AGG	+	chr3.2:71131579-71131598	MS.gene25757:intron	45.0%
	AGAGTCCTCAAGAGGCAGTA+CGG	-	chr3.2:71131116-71131135	None:intergenic	50.0%
	CAATCAACGCATGGTCTCAG+AGG	-	chr3.2:71133609-71133628	None:intergenic	50.0%
	CTCAAGCCCGTTTCAATGCT+TGG	-	chr3.2:71131530-71131549	None:intergenic	50.0%
	GCACACTCTCTTACATCAGG+TGG	-	chr3.2:71133896-71133915	None:intergenic	50.0%
	GCACGACTAGATTTAGCTGG+CGG	+	chr3.2:71133535-71133554	MS.gene25757:intron	50.0%
	GCGCTAACTCAATCGTTAGG+TGG	-	chr3.2:71131244-71131263	None:intergenic	50.0%
	GCTAAAGTATGGGACGGTCA+AGG	+	chr3.2:71133866-71133885	MS.gene25757:CDS	50.0%
	GGGTGTGGATGCATACATCT+TGG	-	chr3.2:71130718-71130737	None:intergenic	50.0%
	GTGGCGCTAACTCAATCGTT+AGG	-	chr3.2:71131247-71131266	None:intergenic	50.0%
	TATAGGGACCAAGGGCAACA+TGG	+	chr3.2:71132507-71132526	MS.gene25757:intron	50.0%
!!	AAGAGGCAGTACGGTGTCAT+TGG	-	chr3.2:71131107-71131126	None:intergenic	50.0%
!!	CGATGCCCAAGCATTGAAAC+GGG	+	chr3.2:71131521-71131540	MS.gene25757:intron	50.0%
!!	GCGATGCCCAAGCATTGAAA+CGG	+	chr3.2:71131520-71131539	MS.gene25757:intron	50.0%
!!	GTCGTGCAAATGATGGACAG+GGG	-	chr3.2:71133522-71133541	None:intergenic	50.0%
	GACCGTCCCATACTTTAGCC+TGG	-	chr3.2:71133865-71133884	None:intergenic	55.0%
	GGACAGGGGATAGCATCGTA+CGG	-	chr3.2:71133508-71133527	None:intergenic	55.0%
	TGACACCGTACTGCCTCTTG+AGG	+	chr3.2:71131108-71131127	MS.gene25757:CDS	55.0%
	TTGTCCAAGTGGGTGGGAAG+TGG	+	chr3.2:71132841-71132860	MS.gene25757:CDS	55.0%
!	CTTGCCACTTCCCACCCACT+TGG	-	chr3.2:71132848-71132867	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	71130707	71133934	71130707	ID=MS.gene25757
chr3.2	mRNA	71130707	71133934	71130707	ID=MS.gene25757.t1;Parent=MS.gene25757
chr3.2	exon	71130707	71130805	71130707	ID=MS.gene25757.t1.exon1;Parent=MS.gene25757.t1
chr3.2	CDS	71130707	71130805	71130707	ID=cds.MS.gene25757.t1;Parent=MS.gene25757.t1
chr3.2	exon	71130944	71131188	71130944	ID=MS.gene25757.t1.exon2;Parent=MS.gene25757.t1
chr3.2	CDS	71130944	71131188	71130944	ID=cds.MS.gene25757.t1;Parent=MS.gene25757.t1
chr3.2	exon	71132075	71132423	71132075	ID=MS.gene25757.t1.exon3;Parent=MS.gene25757.t1
chr3.2	CDS	71132075	71132423	71132075	ID=cds.MS.gene25757.t1;Parent=MS.gene25757.t1
chr3.2	exon	71132512	71133111	71132512	ID=MS.gene25757.t1.exon4;Parent=MS.gene25757.t1
chr3.2	CDS	71132512	71133111	71132512	ID=cds.MS.gene25757.t1;Parent=MS.gene25757.t1
chr3.2	exon	71133362	71133412	71133362	ID=MS.gene25757.t1.exon5;Parent=MS.gene25757.t1
chr3.2	CDS	71133362	71133412	71133362	ID=cds.MS.gene25757.t1;Parent=MS.gene25757.t1
chr3.2	exon	71133737	71133934	71133737	ID=MS.gene25757.t1.exon6;Parent=MS.gene25757.t1
chr3.2	CDS	71133737	71133934	71133737	ID=cds.MS.gene25757.t1;Parent=MS.gene25757.t1

Gene Sequence

>MS.gene25757

ATGGGTATCCAAGATGTATGCATCCACACCCCATTACTCATTGAATTATCAAAGGAATCAAAAGAAACATTCAGAATCTACCCTTTCATCATACAAGGTGTATATATAATAATATGAATGAATATTTTGTCCAATAATTGTCTTCTTATACATGTGATTTTGATTTTGTGCACACCTAGCTAATGGTAATTAATGTTTGATCAATATTTTGTTTTGTTGTTGATTTTGTACATGTAGGAGGATGAGGGAAGATATAAGACAATGGCCTACAATGGAGAATTGAATTTAGGTAACAAATTGCCTATAAGAAGATCAATGCAAGGAGATAGAAATTTGGAGAAGGTTGAAGGAAGTCTTGCAAAAGCAAGAGCATTGATAAAACAAGCCTTGTTGAGAACCAATGACACCGTACTGCCTCTTGAGGACTCTCATGATTATGTTCCACAAGGAGACATCTACAGAAATGCCTTTGCTTTTCATAGGTAGGATTTTATGAACTATATCATGAGAGATAAGGATTTTACTACTCATAATCCACCTAACGATTGAGTTAGCGCCACTTAAGTAACATAGCAAAGTTTTTTTTTTTTCTTAAAGAAAGCTAAACAAATCCACCTAATTGATATCAGGAGAATCGAACCTGCAATATCGAAGTTGTATTAGAGTTTACGTTCAAAGGTCTTAGATTTTTTTTGAACAATCAAAAATGGATTATATATTAACCACAAAAAGCAACTCATCTAGCACAAGATGTGCCAAAAGAATTACCGTAAGAACAAAGAGTCAGTCAAGCAATTAAATACAAACCATTAAGCGATGCCCAAGCATTGAAACGGGCTTGAGCACCACCTATCTGTGCCAAACCTAAAGGCAGCCTTTTTGGCTTTCAACCAGGCTAAAGACATATATTTAACTTTATCTAACAACCTGGGAATAGAAGTTACAACATTATTGAATATGGAGTTGGTCATCCTCATCTTGTGAGCTAACTTTAGTGAGATTCAAATTCATTTAACATAGGTATTGAGCAGTGTAAAATATGATAATGTGAGAATTTAACCCAAGTTTCACGTCATACATGAAAATGAGTATTAAAAATAGTTTCTTAGCTTGGAATGCGCCCATTTAACATTTGTGTTCTCACATTCACACCTCATAACCAAGTGATTGAGCTCAACTGGTTAAACACCTTCATCTTTTGAGCTATCTTTTGGGATGAAATTGAAGCCTATTTAATATGTGGTTGAGATCAAACCATATTTAAGAGTTTTAACCTCTTACATTTTTTTTGAAGGAAGTTTTAACCTCTTACATGTCATGCTCTATTTTTTCATTCTTTTGAATTGTTTTTACATAATGATGATATAGGAGTTACCACTTAATGGAGAAGCTATTTAAAATTTTTGTGTATGAAGAGGGAGAACCTCCTCTTTTTCACTATGGTCCTTGCAAGAATATATATTCAATGGAGGGAATATTTATCAACTTATTGGAAAGCAATACTTTGTTTCGAACTCAGAATCCAAATGAAGCTCATGTCTACTTTCTTCCATTTAGTGTTGTTATGATCCTTGAACATCTCTTTCACCCAGTTATTCGTGACAAAGCTGTTTTGGGAAGAACTATTGGTGATTATGTTCATATCATATCACACAAATATGCATATTGGAATAGAAGTTATGGAGCTGACCATTTCATGCTTTCTTGTCATGATTGGGTACTTATTTTTCAAATTTTCTCCGTTAATTATAGTGTGAGTATGTGACTAATATTTTTATATTTCATGAAAATGGTTTTGTGTATAGGGACCAAGGGCAACATGGTATGTGAAGGAATTGTATTTCATTGCAATTCGAGTAATTTGCAATGCAAATATCTCGGAGCATTTCAATCCAAAGAAAGATGCATCATTTCCTGAAATCAACTTGGTAACAGGAGAAACAACAGGTTTACTTGGTGGCTATCCAACATGGAACAGAACAATTCTGGCTTTCTTTGCAGGACAAATGCATGGTAGAATTAGACCATTGCTTTTCCAACATTGGAAGAACAAAGATAAAGATGTGTTAGTTTATGAAAAACTTCCAGACAAAATTTCATACCATGAAACAATGAAGATGAGTAAATATTGTATTTGTCCAAGTGGGTGGGAAGTGGCAAGTCCAAGAATTGTTGAGGCAATTTATGCTGAATGTGTACCTATTATAATATCACAACAATATGTACTTCCTTTTAGTGATGTTCTTGATTGGGATTCATTTTCTGTTCAAATTGAAGTTAGTGAAATTCCCAAGCTTAAGGAAATTTTCTTGGGTATCTCTGAGGAGAAATATATAAGATTGCAAGAGGGTGTTAAGCAAGTTCAAAGACATTTTGAAGTGAATAATCCTCCTAAGAGATATGATGTATTTCATATGATTATTCATTCTATATGGCTTAGGAGGTTGAATGTACTTGTGAAATAAGTCATAATCTCATTGTGTTTCTCTCAATATTCATTTATTTGTTAATGTGGGTCGTTGCTTGTATTAACAAATGTGAGAAAGATAAATGGAACATTGTTTATATACATGTAGTGACAAATACTTAATTAGTTCTTAATATTTCTACAACAAGTAATCCATATAATCAATTCTTAACGAATTCTTCCGGAAGGAAATGGATGCTGCAATGGATGACTATAAGAAGCGCTGTCGCAACCTAAGTGTACGCATCTATCTATCTATTTCAATTTTGTTTTAATTTCATCTTGTTGTGTTTCACTCTCTTAATTTTGATTCATTCTGGACACAACACAGCCGTACGATGCTATCCCCTGTCCATCATTTGCACGACTAGATTTAGCTGGCGGTATCATACCCTTTCACACATCCGATGCTTATCACCTTATTCTCAAAAACCTCTGAGACCATGCGTTGATTGTTTACAATCCAAAAAATAATGATCAGGTTCAATCCTAAACAGAAGAGAATGAATATTGTTGCATGTTTATTCATTAACCAAACTAATTGTTCATCTTAATTATATTAGGGTCCAACTTTTGTGTTTCATGACCTTGTCAATTGTACCCATACTGTTGGTACTAGTCATAGTACCATGTATTACTTACCTTTCAAGCAAAACCAGAAGAAGATCCTTCCTGCTATAACAACTTTCCAGGCTAAAGTATGGGACGGTCAAGGTAAACCACCTGATGTAAGAGAGTGTGCCATTAAACCAACCACCTGA

Protein sequence

>MS.gene25757.t1

MGIQDVCIHTPLLIELSKESKETFRIYPFIIQGEDEGRYKTMAYNGELNLGNKLPIRRSMQGDRNLEKVEGSLAKARALIKQALLRTNDTVLPLEDSHDYVPQGDIYRNAFAFHRSYHLMEKLFKIFVYEEGEPPLFHYGPCKNIYSMEGIFINLLESNTLFRTQNPNEAHVYFLPFSVVMILEHLFHPVIRDKAVLGRTIGDYVHIISHKYAYWNRSYGADHFMLSCHDWGPRATWYVKELYFIAIRVICNANISEHFNPKKDASFPEINLVTGETTGLLGGYPTWNRTILAFFAGQMHGRIRPLLFQHWKNKDKDVLVYEKLPDKISYHETMKMSKYCICPSGWEVASPRIVEAIYAECVPIIISQQYVLPFSDVLDWDSFSVQIEVSEIPKLKEIFLGISEEKYIRLQEGVKQVQRHFEVNNPPKRYDEMDAAMDDYKKRCRNLSGPTFVFHDLVNCTHTVGTSHSTMYYLPFKQNQKKILPAITTFQAKVWDGQGKPPDVRECAIKPTT