AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28525


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28525.t1 MTR_8g078830 97.727 44 1 0 1 44 1 44 2.33e-23 90.5
MS.gene28525.t1 MTR_5g018680 74.419 43 11 0 1 43 1 43 3.00e-14 67.0
MS.gene28525.t1 MTR_4g068780 68.182 44 14 0 1 44 15 58 1.50e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28525.t1 AT3G50810 63.636 44 16 0 1 44 1 44 1.99e-13 64.7
MS.gene28525.t1 AT3G50810 63.636 44 16 0 1 44 1 44 1.99e-13 64.7
MS.gene28525.t1 AT3G50810 63.636 44 16 0 1 44 1 44 1.99e-13 64.7

Find 37 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATGGTTACTCTGCTTTC+TGG 0.095591 8.4:+27893804 MS.gene28525:CDS
CAGAGAAAGTTGAAACATTT+TGG 0.155943 8.4:+27894612 MS.gene28525:CDS
TCAACTTTCTCTGGTGTTTA+TGG 0.158428 8.4:-27894602 None:intergenic
TACGATTACTTTCGGAGATA+TGG 0.295674 8.4:+27895232 MS.gene28525:CDS
CAAAATGTTTCAACTTTCTC+TGG 0.310351 8.4:-27894611 None:intergenic
TGCTTGGATGTTGATTTCTA+TGG 0.343327 8.4:+27893788 MS.gene28525:CDS
CTCCAGCTTTGCTCTTCGAT+TGG 0.344325 8.4:+27893727 MS.gene28525:CDS
GGTGTTTATGGCCGGCTACT+TGG 0.345172 8.4:-27894590 None:intergenic
TGATGGGTTTGGTAATTTCC+TGG 0.359500 8.4:+27895135 MS.gene28525:CDS
GAGGAATTGCCAGGGGTGTT+AGG 0.368859 8.4:+27893698 MS.gene28525:CDS
TTCCTGGAGCACAACACTAT+TGG 0.376363 8.4:+27895151 MS.gene28525:CDS
TCCAGCTTTGCTCTTCGATT+GGG 0.385905 8.4:+27893728 MS.gene28525:CDS
TTTGGTAATGGTGATGGGTT+TGG 0.404444 8.4:+27895124 MS.gene28525:CDS
CACCAATAGTGTTGTGCTCC+AGG 0.420340 8.4:-27895153 None:intergenic
GCCCAATCGAAGAGCAAAGC+TGG 0.452438 8.4:-27893729 None:intergenic
AAAATGAATCTCCGCTGCTT+CGG 0.454686 8.4:-27895209 None:intergenic
GGTGGAATGGAGGAATTGCC+AGG 0.464393 8.4:+27893689 None:intergenic
TCACCATGTTGAAGTGGCCT+TGG 0.468461 8.4:-27894784 None:intergenic
AGAGAATTACGTTGAATTAG+CGG 0.478315 8.4:+27894548 MS.gene28525:CDS
CTTTCTCTGGTGTTTATGGC+CGG 0.489586 8.4:-27894598 None:intergenic
CTGGAGCACAACACTATTGG+TGG 0.509436 8.4:+27895154 MS.gene28525:CDS
TTAGTTTGTTGCCAAGTAGC+CGG 0.513000 8.4:+27894579 MS.gene28525:CDS
TGCTTCAGAAGATGCTGCAA+TGG 0.533381 8.4:+27895047 MS.gene28525:intron
AAGAGAAGCAGAGGAAAAGA+CGG 0.546272 8.4:-27893756 None:intergenic
AAGCAGAGGAAAAGACGGCT+TGG 0.548579 8.4:-27893751 None:intergenic
TGGTAGTGAGATTGCACTAA+GGG 0.549465 8.4:-27894764 None:intergenic
TCATAATCACCATGTTGAAG+TGG 0.560494 8.4:-27894790 None:intergenic
GAGCTTGTTCCTAACACCCC+TGG 0.567572 8.4:-27893707 None:intergenic
CATGAAGAAAAGAGAAGCAG+AGG 0.574247 8.4:-27893765 None:intergenic
TTGGTAGTGAGATTGCACTA+AGG 0.583096 8.4:-27894765 None:intergenic
GAGCACAACACTATTGGTGG+TGG 0.623288 8.4:+27895157 MS.gene28525:CDS
TCAAAAGTGTACCGAAGCAG+CGG 0.627998 8.4:+27895198 MS.gene28525:CDS
GTGGAATGGAGGAATTGCCA+GGG 0.633206 8.4:+27893690 None:intergenic
CTACCAAGGCCACTTCAACA+TGG 0.636592 8.4:+27894781 MS.gene28525:CDS
ATGCTGCAATGGCAATGTCG+CGG 0.685066 8.4:+27895058 MS.gene28525:CDS
TTAGTGCAATCTCACTACCA+AGG 0.697055 8.4:+27894767 MS.gene28525:CDS
TGGAATGGAGGAATTGCCAG+GGG 0.755495 8.4:+27893691 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTTATCTTCT+GGG + chr8.4:27893872-27893891 MS.gene28525:intron 10.0%
!!! TTTTTTTTTTTTTTATCTTC+TGG + chr8.4:27893871-27893890 MS.gene28525:intron 10.0%
!! CTAAAGAAATAAAAATAGTT+GGG + chr8.4:27894041-27894060 MS.gene28525:intron 15.0%
!! TAAAGAAATAAAAATAGTTG+GGG + chr8.4:27894042-27894061 MS.gene28525:intron 15.0%
!! TCTAAAGAAATAAAAATAGT+TGG + chr8.4:27894040-27894059 MS.gene28525:intron 15.0%
!! AAATACAAGTAAGAAAAAAC+GGG - chr8.4:27893956-27893975 None:intergenic 20.0%
!! CAAATACAAGTAAGAAAAAA+CGG - chr8.4:27893957-27893976 None:intergenic 20.0%
!! TAATCTAAAAATGTCAGAAT+TGG + chr8.4:27894994-27895013 MS.gene28525:intron 20.0%
!! TTGATTGAATTTGTATTGTT+TGG - chr8.4:27894893-27894912 None:intergenic 20.0%
!!! AATATTATGTTTTGGAAGTT+AGG + chr8.4:27894112-27894131 MS.gene28525:intron 20.0%
!!! ATTCTGACATTTTTAGATTA+AGG - chr8.4:27894994-27895013 None:intergenic 20.0%
!!! TGTTTCAACTTTATTTTCAA+AGG + chr8.4:27894161-27894180 MS.gene28525:intron 20.0%
!!! TTCATTTTTACGATTACTTT+CGG + chr8.4:27895224-27895243 MS.gene28525:CDS 20.0%
! AAAATGTCAGAATTGGTAAA+AGG + chr8.4:27895001-27895020 MS.gene28525:intron 25.0%
! TCTTAGAGTTAAGCTAATTA+GGG + chr8.4:27894083-27894102 MS.gene28525:intron 25.0%
!! GGGGTAAAAATATTATGTTT+TGG + chr8.4:27894104-27894123 MS.gene28525:intron 25.0%
!! TTTTCTTACTTGTATTTGCA+TGG + chr8.4:27893959-27893978 MS.gene28525:intron 25.0%
!!! ATAAGGTTTTGATTTTTGGT+TGG + chr8.4:27894487-27894506 MS.gene28525:intron 25.0%
!!! CTTCTATGTCATTTTAAGAA+GGG + chr8.4:27894437-27894456 MS.gene28525:intron 25.0%
!!! TAAGGTTTTGATTTTTGGTT+GGG + chr8.4:27894488-27894507 MS.gene28525:intron 25.0%
!!! TGTCATAAGGTTTTGATTTT+TGG + chr8.4:27894483-27894502 MS.gene28525:intron 25.0%
!!! TTATGAGTTCTGAATATGTT+TGG + chr8.4:27893996-27894015 MS.gene28525:intron 25.0%
!!! TTGAAACATTTTGGCATTAT+CGG + chr8.4:27894621-27894640 MS.gene28525:CDS 25.0%
AGAGAATTACGTTGAATTAG+CGG + chr8.4:27894548-27894567 MS.gene28525:CDS 30.0%
CAAAATGTTTCAACTTTCTC+TGG - chr8.4:27894614-27894633 None:intergenic 30.0%
CTTAGAGTTAAGCTAATTAG+GGG + chr8.4:27894084-27894103 MS.gene28525:intron 30.0%
GTCTTAGAGTTAAGCTAATT+AGG + chr8.4:27894082-27894101 MS.gene28525:intron 30.0%
TGTCTGACATATTTCAATGT+TGG - chr8.4:27894316-27894335 None:intergenic 30.0%
TTAGAGTTAAGCTAATTAGG+GGG + chr8.4:27894085-27894104 MS.gene28525:intron 30.0%
TTATAGCTGATAGCTTAGAA+CGG - chr8.4:27894925-27894944 None:intergenic 30.0%
TTCAGAACTCATAAAATCAC+TGG - chr8.4:27893990-27894009 None:intergenic 30.0%
! AAATAGTTGGGGATTTTACA+AGG + chr8.4:27894053-27894072 MS.gene28525:intron 30.0%
! ATGTACACTGGTCTAATTAT+AGG + chr8.4:27894375-27894394 MS.gene28525:intron 30.0%
! CAGAGAAAGTTGAAACATTT+TGG + chr8.4:27894612-27894631 MS.gene28525:CDS 30.0%
! CATTGATTAGCAATGTCATA+AGG + chr8.4:27894470-27894489 MS.gene28525:intron 30.0%
! TCAGAATTGGTAAAAGGTTT+TGG + chr8.4:27895007-27895026 MS.gene28525:intron 30.0%
!!! AAGGTTTTGATTTTTGGTTG+GGG + chr8.4:27894489-27894508 MS.gene28525:intron 30.0%
!!! GCTTCTATGTCATTTTAAGA+AGG + chr8.4:27894436-27894455 MS.gene28525:intron 30.0%
ACAGCTTCAATATGAAGTGT+CGG + chr8.4:27894342-27894361 MS.gene28525:intron 35.0%
ATGAAGCACAGACACATATA+CGG + chr8.4:27894202-27894221 MS.gene28525:intron 35.0%
CTTACTTGTATTTGCATGGT+GGG + chr8.4:27893963-27893982 MS.gene28525:intron 35.0%
CTTCATATTGAAGCTGTGTT+TGG - chr8.4:27894339-27894358 None:intergenic 35.0%
TAAGATTGCAAAAACAGTGC+TGG - chr8.4:27894718-27894737 None:intergenic 35.0%
TACGATTACTTTCGGAGATA+TGG + chr8.4:27895232-27895251 MS.gene28525:CDS 35.0%
TCAACTTTCTCTGGTGTTTA+TGG - chr8.4:27894605-27894624 None:intergenic 35.0%
TCATAATCACCATGTTGAAG+TGG - chr8.4:27894793-27894812 None:intergenic 35.0%
TCTTACTTGTATTTGCATGG+TGG + chr8.4:27893962-27893981 MS.gene28525:intron 35.0%
! TCTGTTATTGCACAGCTTTT+TGG + chr8.4:27895106-27895125 MS.gene28525:CDS 35.0%
! TTCTCTTTTCTTCATGTGCT+TGG + chr8.4:27893772-27893791 MS.gene28525:CDS 35.0%
!! ATTGCACAGCTTTTTGGTAA+TGG + chr8.4:27895112-27895131 MS.gene28525:CDS 35.0%
!! GAGTTCTGAATATGTTTGGT+AGG + chr8.4:27894000-27894019 MS.gene28525:intron 35.0%
!! TGCTTGGATGTTGATTTCTA+TGG + chr8.4:27893788-27893807 MS.gene28525:CDS 35.0%
!!! AGCTTTTTGGTAATGGTGAT+GGG + chr8.4:27895119-27895138 MS.gene28525:CDS 35.0%
AAAATGAATCTCCGCTGCTT+CGG - chr8.4:27895212-27895231 None:intergenic 40.0%
AAGAGAAGCAGAGGAAAAGA+CGG - chr8.4:27893759-27893778 None:intergenic 40.0%
AGACCAGTGTACATCAATGT+AGG - chr8.4:27894369-27894388 None:intergenic 40.0%
CATGAAGAAAAGAGAAGCAG+AGG - chr8.4:27893768-27893787 None:intergenic 40.0%
TCTATGGTTACTCTGCTTTC+TGG + chr8.4:27893804-27893823 MS.gene28525:CDS 40.0%
TTAGTGCAATCTCACTACCA+AGG + chr8.4:27894767-27894786 MS.gene28525:CDS 40.0%
TTCAATGTTGGACACTGTCA+CGG - chr8.4:27894304-27894323 None:intergenic 40.0%
! TGGTAGTGAGATTGCACTAA+GGG - chr8.4:27894767-27894786 None:intergenic 40.0%
!! ACATTTTGGCATTATCGGAG+AGG + chr8.4:27894626-27894645 MS.gene28525:CDS 40.0%
!! TGATGGGTTTGGTAATTTCC+TGG + chr8.4:27895135-27895154 MS.gene28525:CDS 40.0%
!! TTAGTTTGTTGCCAAGTAGC+CGG + chr8.4:27894579-27894598 MS.gene28525:CDS 40.0%
!! TTGGTAGTGAGATTGCACTA+AGG - chr8.4:27894768-27894787 None:intergenic 40.0%
!! TTTGGTAATGGTGATGGGTT+TGG + chr8.4:27895124-27895143 MS.gene28525:CDS 40.0%
!!! CAGCTTTTTGGTAATGGTGA+TGG + chr8.4:27895118-27895137 MS.gene28525:CDS 40.0%
CTGTGCTTCATAGCGAGTAT+TGG - chr8.4:27894193-27894212 None:intergenic 45.0%
CTTTCTCTGGTGTTTATGGC+CGG - chr8.4:27894601-27894620 None:intergenic 45.0%
GGACCTACATTGATGTACAC+TGG + chr8.4:27894363-27894382 MS.gene28525:intron 45.0%
TCCAGCTTTGCTCTTCGATT+GGG + chr8.4:27893728-27893747 MS.gene28525:CDS 45.0%
TGCTTCAGAAGATGCTGCAA+TGG + chr8.4:27895047-27895066 MS.gene28525:intron 45.0%
TTCCTGGAGCACAACACTAT+TGG + chr8.4:27895151-27895170 MS.gene28525:CDS 45.0%
! TCAAAAGTGTACCGAAGCAG+CGG + chr8.4:27895198-27895217 MS.gene28525:CDS 45.0%
!! ATTGCAGCATCTTCTGAAGC+AGG - chr8.4:27895048-27895067 None:intergenic 45.0%
AAGCAGAGGAAAAGACGGCT+TGG - chr8.4:27893754-27893773 None:intergenic 50.0%
ATGCTGCAATGGCAATGTCG+CGG + chr8.4:27895058-27895077 MS.gene28525:CDS 50.0%
CACCAATAGTGTTGTGCTCC+AGG - chr8.4:27895156-27895175 None:intergenic 50.0%
CTACCAAGGCCACTTCAACA+TGG + chr8.4:27894781-27894800 MS.gene28525:CDS 50.0%
CTCCAGCTTTGCTCTTCGAT+TGG + chr8.4:27893727-27893746 MS.gene28525:CDS 50.0%
CTGGAGCACAACACTATTGG+TGG + chr8.4:27895154-27895173 MS.gene28525:CDS 50.0%
GAGCACAACACTATTGGTGG+TGG + chr8.4:27895157-27895176 MS.gene28525:CDS 50.0%
TCACCATGTTGAAGTGGCCT+TGG - chr8.4:27894787-27894806 None:intergenic 50.0%
GCCCAATCGAAGAGCAAAGC+TGG - chr8.4:27893732-27893751 None:intergenic 55.0%
GGTGTTTATGGCCGGCTACT+TGG - chr8.4:27894593-27894612 None:intergenic 55.0%
! GAGCTTGTTCCTAACACCCC+TGG - chr8.4:27893710-27893729 None:intergenic 55.0%
!! GAGGAATTGCCAGGGGTGTT+AGG + chr8.4:27893698-27893717 MS.gene28525:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 27893695 27895285 27893695 ID=MS.gene28525
chr8.4 mRNA 27893695 27895285 27893695 ID=MS.gene28525.t1;Parent=MS.gene28525
chr8.4 exon 27893695 27893825 27893695 ID=MS.gene28525.t1.exon1;Parent=MS.gene28525.t1
chr8.4 CDS 27893695 27893825 27893695 ID=cds.MS.gene28525.t1;Parent=MS.gene28525.t1
chr8.4 exon 27894545 27894667 27894545 ID=MS.gene28525.t1.exon2;Parent=MS.gene28525.t1
chr8.4 CDS 27894545 27894667 27894545 ID=cds.MS.gene28525.t1;Parent=MS.gene28525.t1
chr8.4 exon 27894763 27894802 27894763 ID=MS.gene28525.t1.exon3;Parent=MS.gene28525.t1
chr8.4 CDS 27894763 27894802 27894763 ID=cds.MS.gene28525.t1;Parent=MS.gene28525.t1
chr8.4 exon 27895055 27895285 27895055 ID=MS.gene28525.t1.exon4;Parent=MS.gene28525.t1
chr8.4 CDS 27895055 27895285 27895055 ID=cds.MS.gene28525.t1;Parent=MS.gene28525.t1
Gene Sequence

>MS.gene28525

ATGGAGGAATTGCCAGGGGTGTTAGGAACAAGCTCCAGCTTTGCTCTTCGATTGGGCCAAGCCGTCTTTTCCTCTGCTTCTCTTTTCTTCATGTGCTTGGATGTTGATTTCTATGGTTACTCTGCTTTCTGGTTAGCATTCTTAATTTTCTGTTTCATATCAAATCATGTTTTTTTTTTTTTTTTTTTTTATCTTCTGGGTTTGTCTTATTTTGATGTTGCTTATGTAAAGTTTGTAACTTTATCAATTTTACTTTTGCCCGTTTTTTCTTACTTGTATTTGCATGGTGGGTCCAGTGATTTTATGAGTTCTGAATATGTTTGGTAGGATTGATCAATGAGAAATTCTAAAGAAATAAAAATAGTTGGGGATTTTACAAGGAGTGTTGTCTTAGAGTTAAGCTAATTAGGGGGTAAAAATATTATGTTTTGGAAGTTAGGACAGTTTTTGTGTGCTGCAAATATATTGTTTCAACTTTATTTTCAAAGGCAAGTACCAATACTCGCTATGAAGCACAGACACATATACGGACACTGATAGCGTCACATCGACGCTGATAATAAATTGAAAAATTAAAGTAATTGAATGTAATTACATATGTTGATGCCGTGACAGTGTCCAACATTGAAATATGTCAGACACCAAACACAGCTTCAATATGAAGTGTCGGACCTACATTGATGTACACTGGTCTAATTATAGGCTAAATAATGCATGTATGCTTGCTTTGTTATTGAATTTGCTTCTATGTCATTTTAAGAAGGGTCATTCTAAACATTGATTAGCAATGTCATAAGGTTTTGATTTTTGGTTGGGGAAATTATGTTACTAAATCATATTCATTTATTAGTGAAGAGAATTACGTTGAATTAGCGGCTAGCGAATTAGTTTGTTGCCAAGTAGCCGGCCATAAACACCAGAGAAAGTTGAAACATTTTGGCATTATCGGAGAGGAAATGATTTCAAGCTGTTTGTAAGTTGAAGTGAAATCTTTGTATAATTGACTTTCATATTAATTTGCCAGCACTGTTTTTGCAATCTTAACTTCACTGTTTATTTTTTCATTAGTCCCTTAGTGCAATCTCACTACCAAGGCCACTTCAACATGGTGATTATGATAAGAGTGAATGTTGTTTTTCTTCTGAAAAGAATGTTTGTATCACAAGTTTATAAGCTAGTTCAGCTGTTAATTTTACCAAACAATACAAATTCAATCAACTAGCTTATCCGTTCTAAGCTATCAGCTATAAGCTAACTTATCAGTTATCAGCTGTTTTTTTTTTTTATCAAACAGAGCCTTAATCTAAAAATGTCAGAATTGGTAAAAGGTTTTGGTATAAACATGTTTTTCCTGCTTCAGAAGATGCTGCAATGGCAATGTCGCGGAGTTTATCTTGTCTTGATTATCTTTTCTGTTATTGCACAGCTTTTTGGTAATGGTGATGGGTTTGGTAATTTCCTGGAGCACAACACTATTGGTGGTGGATGCATATTCTATCTTTATCAAAAGTGTACCGAAGCAGCGGAGATTCATTTTTACGATTACTTTCGGAGATATGGTATGTACGCTTTACAACTGAAAAAGACTTAA

Protein sequence

>MS.gene28525.t1

MEELPGVLGTSSSFALRLGQAVFSSASLFFMCLDVDFYGYSAFCEENYVELAASELVCCQVAGHKHQRKLKHFGIIGEEMISSCFPLVQSHYQGHFNMKMLQWQCRGVYLVLIIFSVIAQLFGNGDGFGNFLEHNTIGGGCIFYLYQKCTEAAEIHFYDYFRRYGMYALQLKKT