AlfalfaGEDB | MS.gene31318

M. sativa cultivar XinJiangDaYe / MS.gene31318

KEGG: K09481
GO: GO:0005575 GO:0005784 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0015031 GO:0015833 GO:0032991 GO:0033036 GO:0042886 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0071256 GO:0071702 GO:0071705 GO:0098796
PFAM: PF03911.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31318.t1	XP_003597194.1	100	46	0	0	62	107	62	107	9.30E-17	96.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31318.t1	P38389	73.2	41	11	0	62	102	42	82	1.2e-09	63.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31318.t1	Q2HSW3	100.0	46	0	0	62	107	62	107	6.7e-17	96.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049356	MS.gene31318	-0.806743	7.09E-50	-1.69E-46
MS.gene050593	MS.gene31318	-0.814669	1.37E-51	-1.69E-46
MS.gene050670	MS.gene31318	-0.834115	3.61E-56	-1.69E-46
MS.gene050747	MS.gene31318	-0.813483	2.50E-51	-1.69E-46
MS.gene050950	MS.gene31318	-0.803552	3.30E-49	-1.69E-46
MS.gene051167	MS.gene31318	0.813307	2.74E-51	-1.69E-46
MS.gene051360	MS.gene31318	-0.806421	8.29E-50	-1.69E-46
MS.gene051387	MS.gene31318	-0.829625	4.64E-55	-1.69E-46
MS.gene051525	MS.gene31318	-0.839886	1.21E-57	-1.69E-46
MS.gene051838	MS.gene31318	-0.82442	8.15E-54	-1.69E-46
MS.gene052011	MS.gene31318	0.822065	2.89E-53	-1.69E-46
MS.gene052346	MS.gene31318	-0.834801	2.43E-56	-1.69E-46
MS.gene052414	MS.gene31318	-0.801508	8.71E-49	-1.69E-46
MS.gene052421	MS.gene31318	-0.81632	5.88E-52	-1.69E-46
MS.gene053169	MS.gene31318	-0.829131	6.11E-55	-1.69E-46
MS.gene053318	MS.gene31318	-0.828786	7.41E-55	-1.69E-46
MS.gene053824	MS.gene31318	-0.810495	1.12E-50	-1.69E-46
MS.gene054486	MS.gene31318	-0.826374	2.81E-54	-1.69E-46
MS.gene054556	MS.gene31318	-0.809146	2.19E-50	-1.69E-46
MS.gene05494	MS.gene31318	-0.817985	2.48E-52	-1.69E-46
MS.gene05505	MS.gene31318	-0.819392	1.19E-52	-1.69E-46
MS.gene055486	MS.gene31318	-0.824855	6.44E-54	-1.69E-46
MS.gene055741	MS.gene31318	-0.810857	9.36E-51	-1.69E-46
MS.gene055744	MS.gene31318	-0.811822	5.78E-51	-1.69E-46
MS.gene055909	MS.gene31318	-0.855658	5.44E-62	-1.69E-46
MS.gene055911	MS.gene31318	-0.855907	4.60E-62	-1.69E-46
MS.gene055941	MS.gene31318	-0.803742	3.01E-49	-1.69E-46
MS.gene05601	MS.gene31318	-0.838117	3.48E-57	-1.69E-46
MS.gene056166	MS.gene31318	-0.80279	4.75E-49	-1.69E-46
MS.gene056448	MS.gene31318	-0.801923	7.16E-49	-1.69E-46
MS.gene056511	MS.gene31318	-0.838985	2.08E-57	-1.69E-46
MS.gene056514	MS.gene31318	-0.808991	2.36E-50	-1.69E-46
MS.gene057019	MS.gene31318	-0.802795	4.74E-49	-1.69E-46
MS.gene057289	MS.gene31318	-0.8214	4.12E-53	-1.69E-46
MS.gene057734	MS.gene31318	-0.821746	3.43E-53	-1.69E-46
MS.gene057856	MS.gene31318	-0.840203	1.00E-57	-1.69E-46
MS.gene058007	MS.gene31318	-0.817816	2.71E-52	-1.69E-46
MS.gene058120	MS.gene31318	-0.825653	4.17E-54	-1.69E-46
MS.gene058157	MS.gene31318	-0.803209	3.89E-49	-1.69E-46
MS.gene058376	MS.gene31318	-0.821363	4.20E-53	-1.69E-46
MS.gene058444	MS.gene31318	-0.82157	3.76E-53	-1.69E-46
MS.gene058700	MS.gene31318	-0.850481	1.65E-60	-1.69E-46
MS.gene058948	MS.gene31318	-0.820469	6.75E-53	-1.69E-46
MS.gene059420	MS.gene31318	-0.832047	1.18E-55	-1.69E-46
MS.gene059674	MS.gene31318	-0.813012	3.17E-51	-1.69E-46
MS.gene059749	MS.gene31318	-0.806437	8.23E-50	-1.69E-46
MS.gene060372	MS.gene31318	-0.817126	3.88E-52	-1.69E-46
MS.gene060837	MS.gene31318	-0.867995	9.07E-66	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31318.t1	MTR_2g093770	100.000	107	0	0	1	107	1	107	1.25e-71	208
MS.gene31318.t1	MTR_1g082400	72.826	92	20	2	15	104	17	105	2.80e-36	119
MS.gene31318.t1	MTR_8g086330	60.976	82	26	1	21	102	4	79	7.47e-25	89.7
MS.gene31318.t1	MTR_4g028080	61.446	83	26	1	21	103	4	80	4.42e-24	87.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31318.t1	AT5G60460	77.907	86	17	1	22	105	22	107	3.53e-37	122
MS.gene31318.t1	AT5G60460	77.907	86	17	1	22	105	22	107	3.53e-37	122
MS.gene31318.t1	AT2G45070	60.759	79	29	1	24	102	6	82	2.38e-23	85.9
MS.gene31318.t1	AT2G45070	60.759	79	29	1	24	102	6	82	2.38e-23	85.9
MS.gene31318.t1	AT2G45070	60.759	79	29	1	24	102	6	82	2.38e-23	85.9
MS.gene31318.t1	AT2G45070	60.759	79	29	1	24	102	6	82	2.38e-23	85.9
MS.gene31318.t1	AT3G60540	57.831	83	30	1	21	103	3	80	2.76e-23	85.9
MS.gene31318.t1	AT3G60540	57.831	83	30	1	21	103	3	80	2.76e-23	85.9
MS.gene31318.t1	AT3G60540	57.831	83	30	1	21	103	3	80	2.76e-23	85.9

Find 41 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACGCTGGCGGTGCTGTTTC+CGG	0.263401	2.4:+8254620	None:intergenic
GATTTCACCGACGGTGGTTT+TGG	0.294926	2.4:-8254532	MS.gene31318:CDS
GTCACCGCGCTTCATGTGTT+CGG	0.324439	2.4:-8254483	MS.gene31318:CDS
TCACCGCGCTTCATGTGTTC+GGG	0.377638	2.4:-8254482	MS.gene31318:CDS
TCCTCCTGGACGGGAGGATG+TGG	0.389528	2.4:+8254710	None:intergenic
GGCTCCGCTGCCGCCACAGC+CGG	0.408056	2.4:-8254666	MS.gene31318:CDS
CATGCCGGCTGTGGCGGCAG+CGG	0.430788	2.4:+8254662	None:intergenic
GCAGCGAGTCCGCCTCCTCC+TGG	0.437060	2.4:+8254696	None:intergenic
AAGCTTTATCGTTACAAAGC+TGG	0.460509	2.4:-8254459	MS.gene31318:CDS
TCCTCCCGTCCAGGAGGAGG+CGG	0.463865	2.4:-8254705	MS.gene31318:CDS
ACATCCTCCCGTCCAGGAGG+AGG	0.475747	2.4:-8254708	MS.gene31318:CDS
ACCTCCACATCCTCCCGTCC+AGG	0.490070	2.4:-8254714	MS.gene31318:CDS
AACAGCACCGCCAGCGTTGG+AGG	0.491709	2.4:-8254615	MS.gene31318:CDS
TATCGTTACAAAGCTGGCGC+TGG	0.497594	2.4:-8254453	MS.gene31318:CDS
AGCACCGCCAGCGTTGGAGG+TGG	0.498786	2.4:-8254612	MS.gene31318:CDS
CTCCCACTCATCATGGCTAA+AGG	0.505690	2.4:-8254759	None:intergenic
TCGACGACGCATGCCGGCTG+TGG	0.506267	2.4:+8254653	None:intergenic
ACGACGCATGCCGGCTGTGG+CGG	0.508943	2.4:+8254656	None:intergenic
GCTAGATCCACCTCCAACGC+TGG	0.511643	2.4:+8254605	None:intergenic
CGTCGTCGACGTCTCACCTC+CGG	0.514572	2.4:-8254639	MS.gene31318:CDS
ATTCTACACTGACGATGCAC+CGG	0.515056	2.4:-8254562	MS.gene31318:CDS
TTCTACACTGACGATGCACC+GGG	0.517664	2.4:-8254561	MS.gene31318:CDS
CGAGTCCGCCTCCTCCTGGA+CGG	0.525894	2.4:+8254700	None:intergenic
GGAAACAGCACCGCCAGCGT+TGG	0.527583	2.4:-8254618	MS.gene31318:CDS
TCCTGGACGGGAGGATGTGG+AGG	0.527989	2.4:+8254713	None:intergenic
GAGTCCGCCTCCTCCTGGAC+GGG	0.535624	2.4:+8254701	None:intergenic
TCCGCCTCCTCCTGGACGGG+AGG	0.535956	2.4:+8254704	None:intergenic
GTTATGAGTCTCTGCTTCAT+CGG	0.539263	2.4:-8254510	MS.gene31318:CDS
TTTGAAGATTTCACCGACGG+TGG	0.598789	2.4:-8254538	MS.gene31318:CDS
GAGACGTCGACGACGCATGC+CGG	0.602996	2.4:+8254647	None:intergenic
GTGGCGGCAGCGGAGCCACG+TGG	0.617814	2.4:+8254672	None:intergenic
CTCATAACCAAAACCACCGT+CGG	0.617981	2.4:+8254525	None:intergenic
GCTGGCGGTGCTGTTTCCGG+AGG	0.626090	2.4:+8254623	None:intergenic
AGATCCACCTCCAACGCTGG+CGG	0.627371	2.4:+8254608	None:intergenic
AGCGTTGGAGGTGGATCTAG+CGG	0.636568	2.4:-8254603	MS.gene31318:CDS
GTCGGTGAAATCTTCAAACC+CGG	0.639473	2.4:+8254543	None:intergenic
GGGTTTGAAGATTTCACCGA+CGG	0.641586	2.4:-8254541	MS.gene31318:CDS
GGCGGACTCGCTGCACCACG+TGG	0.650379	2.4:-8254687	MS.gene31318:CDS
TCCACATCCTCCCGTCCAGG+AGG	0.666674	2.4:-8254711	MS.gene31318:CDS
GTTGGAGGTGGATCTAGCGG+AGG	0.684640	2.4:-8254600	MS.gene31318:CDS
CTTCCCGAACACATGAAGCG+CGG	0.732527	2.4:+8254479	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAGCTTTATCGTTACAAAGC+TGG	-	chr2.4:8254734-8254753	MS.gene31318:CDS	35.0%
	GTTATGAGTCTCTGCTTCAT+CGG	-	chr2.4:8254683-8254702	MS.gene31318:CDS	40.0%
	ATTCTACACTGACGATGCAC+CGG	-	chr2.4:8254631-8254650	MS.gene31318:CDS	45.0%
	CTCATAACCAAAACCACCGT+CGG	+	chr2.4:8254671-8254690	None:intergenic	45.0%
	GGGTTTGAAGATTTCACCGA+CGG	-	chr2.4:8254652-8254671	MS.gene31318:CDS	45.0%
	GTCGGTGAAATCTTCAAACC+CGG	+	chr2.4:8254653-8254672	None:intergenic	45.0%
	TTTGAAGATTTCACCGACGG+TGG	-	chr2.4:8254655-8254674	MS.gene31318:CDS	45.0%
	TATCGTTACAAAGCTGGCGC+TGG	-	chr2.4:8254740-8254759	MS.gene31318:CDS	50.0%
	TTCTACACTGACGATGCACC+GGG	-	chr2.4:8254632-8254651	MS.gene31318:CDS	50.0%
!	GATTTCACCGACGGTGGTTT+TGG	-	chr2.4:8254661-8254680	MS.gene31318:CDS	50.0%
	CTTCCCGAACACATGAAGCG+CGG	+	chr2.4:8254717-8254736	None:intergenic	55.0%
	GTCACCGCGCTTCATGTGTT+CGG	-	chr2.4:8254710-8254729	MS.gene31318:CDS	55.0%
	TCACCGCGCTTCATGTGTTC+GGG	-	chr2.4:8254711-8254730	MS.gene31318:CDS	55.0%
!	AGCGTTGGAGGTGGATCTAG+CGG	-	chr2.4:8254590-8254609	MS.gene31318:CDS	55.0%
	AGATCCACCTCCAACGCTGG+CGG	+	chr2.4:8254588-8254607	None:intergenic	60.0%
	GCTAGATCCACCTCCAACGC+TGG	+	chr2.4:8254591-8254610	None:intergenic	60.0%
	GTTGGAGGTGGATCTAGCGG+AGG	-	chr2.4:8254593-8254612	MS.gene31318:CDS	60.0%
!!	AACGCTGGCGGTGCTGTTTC+CGG	+	chr2.4:8254576-8254595	None:intergenic	60.0%
	ACATCCTCCCGTCCAGGAGG+AGG	-	chr2.4:8254485-8254504	MS.gene31318:CDS	65.0%
	ACCTCCACATCCTCCCGTCC+AGG	-	chr2.4:8254479-8254498	MS.gene31318:CDS	65.0%
	CGTCGTCGACGTCTCACCTC+CGG	-	chr2.4:8254554-8254573	MS.gene31318:CDS	65.0%
	GAGACGTCGACGACGCATGC+CGG	+	chr2.4:8254549-8254568	None:intergenic	65.0%
	GGAAACAGCACCGCCAGCGT+TGG	-	chr2.4:8254575-8254594	MS.gene31318:CDS	65.0%
	TCCACATCCTCCCGTCCAGG+AGG	-	chr2.4:8254482-8254501	MS.gene31318:CDS	65.0%
	TCCTCCTGGACGGGAGGATG+TGG	+	chr2.4:8254486-8254505	None:intergenic	65.0%
	TCCTGGACGGGAGGATGTGG+AGG	+	chr2.4:8254483-8254502	None:intergenic	65.0%
!	AACAGCACCGCCAGCGTTGG+AGG	-	chr2.4:8254578-8254597	MS.gene31318:CDS	65.0%
	CGAGTCCGCCTCCTCCTGGA+CGG	+	chr2.4:8254496-8254515	None:intergenic	70.0%
	GAGTCCGCCTCCTCCTGGAC+GGG	+	chr2.4:8254495-8254514	None:intergenic	70.0%
	TCCTCCCGTCCAGGAGGAGG+CGG	-	chr2.4:8254488-8254507	MS.gene31318:CDS	70.0%
!	ACGACGCATGCCGGCTGTGG+CGG	+	chr2.4:8254540-8254559	None:intergenic	70.0%
!	AGCACCGCCAGCGTTGGAGG+TGG	-	chr2.4:8254581-8254600	MS.gene31318:CDS	70.0%
!	TCGACGACGCATGCCGGCTG+TGG	+	chr2.4:8254543-8254562	None:intergenic	70.0%
!!	GCTGGCGGTGCTGTTTCCGG+AGG	+	chr2.4:8254573-8254592	None:intergenic	70.0%
	GCAGCGAGTCCGCCTCCTCC+TGG	+	chr2.4:8254500-8254519	None:intergenic	75.0%
	TCCGCCTCCTCCTGGACGGG+AGG	+	chr2.4:8254492-8254511	None:intergenic	75.0%
!	CATGCCGGCTGTGGCGGCAG+CGG	+	chr2.4:8254534-8254553	None:intergenic	75.0%
!	GGCGGACTCGCTGCACCACG+TGG	-	chr2.4:8254506-8254525	MS.gene31318:CDS	75.0%
!	GGCTCCGCTGCCGCCACAGC+CGG	-	chr2.4:8254527-8254546	MS.gene31318:CDS	80.0%
!	GTGGCGGCAGCGGAGCCACG+TGG	+	chr2.4:8254524-8254543	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	8254446	8254769	8254446	ID=MS.gene31318
chr2.4	mRNA	8254446	8254769	8254446	ID=MS.gene31318.t1;Parent=MS.gene31318
chr2.4	exon	8254446	8254769	8254446	ID=MS.gene31318.t1.exon1;Parent=MS.gene31318.t1
chr2.4	CDS	8254446	8254769	8254446	ID=cds.MS.gene31318.t1;Parent=MS.gene31318.t1

Gene Sequence	>MS.gene31318 ATGGCTAAAGGCGCATCTCAATCTCAATCATCAACCTCCACATCCTCCCGTCCAGGAGGAGGCGGACTCGCTGCACCACGTGGCTCCGCTGCCGCCACAGCCGGCATGCGTCGTCGACGTCTCACCTCCGGAAACAGCACCGCCAGCGTTGGAGGTGGATCTAGCGGAGGAAGCAACATGCTGAGATTCTACACTGACGATGCACCGGGTTTGAAGATTTCACCGACGGTGGTTTTGGTTATGAGTCTCTGCTTCATCGGTTTCGTCACCGCGCTTCATGTGTTCGGGAAGCTTTATCGTTACAAAGCTGGCGCTGGTGTATAA
Protein sequence	>MS.gene31318.t1 MAKGASQSQSSTSTSSRPGGGGLAAPRGSAAATAGMRRRRLTSGNSTASVGGGSSGGSNMLRFYTDDAPGLKISPTVVLVMSLCFIGFVTALHVFGKLYRYKAGAGV