AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35557


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 61 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCACGGCTATATTATTT+TGG 0.224338 2.3:+12090229 MS.gene35557:CDS
AAATATTGTAGGAAAATTTC+AGG 0.231634 2.3:+12090281 MS.gene35557:CDS
TGAGAATGTGCAAGGTTATT+TGG 0.272129 2.3:-12090045 None:intergenic
TCACTTTCATGTGAATTTAC+TGG 0.292583 2.3:-12089581 None:intergenic
CACAACGGAGAATACGATTA+AGG 0.296370 2.3:+12090085 MS.gene35557:CDS
CATTCTGTCCTTTCACCCTT+AGG 0.318522 2.3:-12089292 None:intergenic
CATCAAACGATTGGTCATTC+TGG 0.319286 2.3:-12090184 None:intergenic
AAAAGAGCGCAACGTGAAAT+CGG 0.320485 2.3:+12089216 MS.gene35557:CDS
ATGTATGTTTCAGGATTCTT+TGG 0.329361 2.3:+12089439 MS.gene35557:intron
ATAACTGCTGCTTCCCCGTT+TGG 0.351801 2.3:-12089681 None:intergenic
CAGTTATGGTTCAGCTTCAA+TGG 0.373075 2.3:+12089697 MS.gene35557:CDS
GAGCTACTGACTGTGGCTAT+TGG 0.383824 2.3:+12089182 MS.gene35557:CDS
TCACTTAGGTCGTGCACCTA+AGG 0.393121 2.3:+12089276 MS.gene35557:CDS
CACTTTCATGTGAATTTACT+GGG 0.402866 2.3:-12089580 None:intergenic
ATCTGATTGAATGAATGATT+TGG 0.404293 2.3:-12089098 None:intergenic
TTCTCCGTTGTGCAGTGCCT+TGG 0.417997 2.3:-12090074 None:intergenic
AAACAAGTTAGAAATATTGT+AGG 0.438564 2.3:+12090270 MS.gene35557:CDS
AAATCGGGTTCTAATGTCAT+AGG 0.442652 2.3:+12089232 MS.gene35557:CDS
AAAGAGCGCAACGTGAAATC+GGG 0.454109 2.3:+12089217 MS.gene35557:CDS
CGAACTTTGGTGTTTCACTT+AGG 0.463686 2.3:+12089262 MS.gene35557:CDS
ATTGTTATGCTGAGATACTA+AGG 0.473886 2.3:+12089968 MS.gene35557:CDS
GGTTGATGGTAGTTCCAAAC+GGG 0.476342 2.3:+12089667 MS.gene35557:CDS
AGCAAATATCATCAAACGAT+TGG 0.476940 2.3:-12090193 None:intergenic
AAACGGGGAAGCAGCAGTTA+TGG 0.485441 2.3:+12089683 MS.gene35557:CDS
TCTGGCAAAGAAGCTACTCT+TGG 0.501988 2.3:-12090166 None:intergenic
ACAACGGAGAATACGATTAA+GGG 0.503506 2.3:+12090086 MS.gene35557:CDS
GAACAAGAAAAGGAGGTTGA+TGG 0.507935 2.3:+12089653 MS.gene35557:CDS
CGGTACTGATCATAGAGAAA+GGG 0.508013 2.3:+12089622 MS.gene35557:CDS
AGACTCTTCGGGCCAGCTAA+AGG 0.513022 2.3:+12089778 MS.gene35557:CDS
GGGTGTCTGCGAACAAGAAA+AGG 0.514695 2.3:+12089643 MS.gene35557:CDS
CATAGGTACAAAGCGAACTT+TGG 0.517811 2.3:+12089249 MS.gene35557:CDS
CACTTAGGTCGTGCACCTAA+GGG 0.525784 2.3:+12089277 MS.gene35557:CDS
TCACTTTCATGTGAATGTAC+AGG 0.533418 2.3:-12089539 None:intergenic
GCGGTACTGATCATAGAGAA+AGG 0.535249 2.3:+12089621 MS.gene35557:CDS
CTGTGTCAGTGACAGGTCTC+CGG 0.535633 2.3:+12089330 MS.gene35557:CDS
CGTTGTGCAGTGCCTTGGCA+TGG 0.537314 2.3:-12090069 None:intergenic
TGGTTGTTTGTCGTCTCAAG+AGG 0.540094 2.3:+12089459 MS.gene35557:CDS
CGTGGAAGAAAATATCCAAA+TGG 0.543612 2.3:+12089145 MS.gene35557:CDS
AGGTTGATGGTAGTTCCAAA+CGG 0.545938 2.3:+12089666 MS.gene35557:CDS
ACGAGTACTGTGTCAGTGAC+AGG 0.551156 2.3:+12089323 MS.gene35557:CDS
TGTGGCTATTGGAAAGCCAC+TGG 0.560417 2.3:+12089193 MS.gene35557:CDS
CAAAGAAGCTACTCTTGGAC+TGG 0.563143 2.3:-12090161 None:intergenic
TGTTATGTAGATGGATGAAG+AGG 0.570365 2.3:+12089945 MS.gene35557:intron
GTCGTGCACCTAAGGGTGAA+AGG 0.588146 2.3:+12089284 MS.gene35557:CDS
CTAAGGGTGAAAGGACAGAA+TGG 0.589250 2.3:+12089293 MS.gene35557:CDS
GATGAATCATCAATGTCTCA+AGG 0.597395 2.3:+12090009 MS.gene35557:CDS
AGTGACTGTGCTATCTCAGA+AGG 0.605478 2.3:+12089599 MS.gene35557:CDS
TGGTTCTTCTTCTCTCCCCG+TGG 0.613681 2.3:+12089127 MS.gene35557:CDS
GCACATTCTCATCCATGCCA+AGG 0.629279 2.3:+12090057 MS.gene35557:CDS
GCTCCTGGTTGAGAATGTGT+TGG 0.632511 2.3:-12090117 None:intergenic
TCATTCAATCAGATAACGAG+TGG 0.635826 2.3:+12089107 MS.gene35557:CDS
GACTGTGCTATCTCAGAAGG+CGG 0.647418 2.3:+12089602 MS.gene35557:CDS
AATCAGCGACCAGCTAATGA+CGG 0.647955 2.3:+12089746 MS.gene35557:CDS
AGTAAAAGAGCTACTGACTG+TGG 0.648863 2.3:+12089175 MS.gene35557:CDS
GGTACTGATCATAGAGAAAG+GGG 0.649597 2.3:+12089623 MS.gene35557:CDS
TCACATGAAAGTGATTGTGC+TGG 0.661055 2.3:+12089548 MS.gene35557:CDS
AAACCAACACATTCTCAACC+AGG 0.668307 2.3:+12090114 MS.gene35557:CDS
TGTCTGCGAACAAGAAAAGG+AGG 0.695006 2.3:+12089646 MS.gene35557:CDS
GGCATGGATGAGAATGTGCA+AGG 0.698005 2.3:-12090053 None:intergenic
CATGCCAAGGCACTGCACAA+CGG 0.707247 2.3:+12090070 MS.gene35557:CDS
GTTGATGGTAGTTCCAAACG+GGG 0.760252 2.3:+12089668 MS.gene35557:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACAAGTTAGAAATATTGT+AGG + chr2.3:12090270-12090289 MS.gene35557:CDS 20.0%
!! AAATATTGTAGGAAAATTTC+AGG + chr2.3:12090281-12090300 MS.gene35557:CDS 20.0%
!! CATAACAATATCATCATAAA+AGG - chr2.3:12089932-12089951 None:intergenic 20.0%
!!! ATATTATTTTGGTCTTCATT+TGG + chr2.3:12090240-12090259 MS.gene35557:CDS 20.0%
!!! CTTGTTTTTTAACATTCATA+GGG - chr2.3:12089386-12089405 None:intergenic 20.0%
!!! TCTTGTTTTTTAACATTCAT+AGG - chr2.3:12089387-12089406 None:intergenic 20.0%
! ATCTGATTGAATGAATGATT+TGG - chr2.3:12089101-12089120 None:intergenic 25.0%
! TGATGATATTGTTATGTAGA+TGG + chr2.3:12089936-12089955 MS.gene35557:intron 25.0%
!! ATCAATTTTATACCTTTAGC+TGG - chr2.3:12089793-12089812 None:intergenic 25.0%
!!! TTGTTTGTTATGTATGTTTC+AGG + chr2.3:12089430-12089449 MS.gene35557:intron 25.0%
AGCAAATATCATCAAACGAT+TGG - chr2.3:12090196-12090215 None:intergenic 30.0%
ATGTATGTTTCAGGATTCTT+TGG + chr2.3:12089439-12089458 MS.gene35557:intron 30.0%
ATTGTTATGCTGAGATACTA+AGG + chr2.3:12089968-12089987 MS.gene35557:CDS 30.0%
CACTTTCATGTGAATTTACT+GGG - chr2.3:12089583-12089602 None:intergenic 30.0%
TCACTTTCATGTGAATTTAC+TGG - chr2.3:12089584-12089603 None:intergenic 30.0%
! ATTTGGATATTTTCTTCCAC+GGG - chr2.3:12089146-12089165 None:intergenic 30.0%
! CATTTGGATATTTTCTTCCA+CGG - chr2.3:12089147-12089166 None:intergenic 30.0%
! CTTTTACTTTGTGAACCATT+TGG - chr2.3:12089163-12089182 None:intergenic 30.0%
! TTTCTTCATTTTCACTCTCA+CGG + chr2.3:12090215-12090234 MS.gene35557:CDS 30.0%
! TTTTTAACATTCATAGGGAG+AGG - chr2.3:12089381-12089400 None:intergenic 30.0%
!!! TTATTTTGGTCTTCATTTGG+TGG + chr2.3:12090243-12090262 MS.gene35557:CDS 30.0%
ACAACGGAGAATACGATTAA+GGG + chr2.3:12090086-12090105 MS.gene35557:CDS 35.0%
ACATTCAGATTATTACCCAC+CGG - chr2.3:12089352-12089371 None:intergenic 35.0%
CGTGGAAGAAAATATCCAAA+TGG + chr2.3:12089145-12089164 MS.gene35557:CDS 35.0%
CGTGTAATGTACTCATTCTA+CGG - chr2.3:12089846-12089865 None:intergenic 35.0%
GATGAATCATCAATGTCTCA+AGG + chr2.3:12090009-12090028 MS.gene35557:CDS 35.0%
TCACTTTCATGTGAATGTAC+AGG - chr2.3:12089542-12089561 None:intergenic 35.0%
TCATTCAATCAGATAACGAG+TGG + chr2.3:12089107-12089126 MS.gene35557:CDS 35.0%
TGAGAATGTGCAAGGTTATT+TGG - chr2.3:12090048-12090067 None:intergenic 35.0%
TGTTATGTAGATGGATGAAG+AGG + chr2.3:12089945-12089964 MS.gene35557:intron 35.0%
! AAATCGGGTTCTAATGTCAT+AGG + chr2.3:12089232-12089251 MS.gene35557:CDS 35.0%
! CTAGATTCAGCTTTTTGCAT+CGG + chr2.3:12089878-12089897 MS.gene35557:intron 35.0%
! CTCTCACGGCTATATTATTT+TGG + chr2.3:12090229-12090248 MS.gene35557:CDS 35.0%
! TTTGGATATTTTCTTCCACG+GGG - chr2.3:12089145-12089164 None:intergenic 35.0%
AAACCAACACATTCTCAACC+AGG + chr2.3:12090114-12090133 MS.gene35557:CDS 40.0%
CACAACGGAGAATACGATTA+AGG + chr2.3:12090085-12090104 MS.gene35557:CDS 40.0%
CAGTTATGGTTCAGCTTCAA+TGG + chr2.3:12089697-12089716 MS.gene35557:CDS 40.0%
CATAGGTACAAAGCGAACTT+TGG + chr2.3:12089249-12089268 MS.gene35557:CDS 40.0%
GAACAAGAAAAGGAGGTTGA+TGG + chr2.3:12089653-12089672 MS.gene35557:CDS 40.0%
GTCAAAAAACCGTCATTAGC+TGG - chr2.3:12089758-12089777 None:intergenic 40.0%
TAACATTCATAGGGAGAGGA+AGG - chr2.3:12089377-12089396 None:intergenic 40.0%
TCACATGAAAGTGATTGTGC+TGG + chr2.3:12089548-12089567 MS.gene35557:CDS 40.0%
! AAAAGAGCGCAACGTGAAAT+CGG + chr2.3:12089216-12089235 MS.gene35557:CDS 40.0%
! AGTAAAAGAGCTACTGACTG+TGG + chr2.3:12089175-12089194 MS.gene35557:CDS 40.0%
! CATCAAACGATTGGTCATTC+TGG - chr2.3:12090187-12090206 None:intergenic 40.0%
!! AGGTTGATGGTAGTTCCAAA+CGG + chr2.3:12089666-12089685 MS.gene35557:CDS 40.0%
!! CGAACTTTGGTGTTTCACTT+AGG + chr2.3:12089262-12089281 MS.gene35557:CDS 40.0%
!! CGGTACTGATCATAGAGAAA+GGG + chr2.3:12089622-12089641 MS.gene35557:CDS 40.0%
!! GGTACTGATCATAGAGAAAG+GGG + chr2.3:12089623-12089642 MS.gene35557:CDS 40.0%
!!! CGGTTTTTTGACAGACTCTT+CGG + chr2.3:12089766-12089785 MS.gene35557:CDS 40.0%
!!! GGTTTTTTGACAGACTCTTC+GGG + chr2.3:12089767-12089786 MS.gene35557:CDS 40.0%
AATCAGCGACCAGCTAATGA+CGG + chr2.3:12089746-12089765 MS.gene35557:CDS 45.0%
AGTGACTGTGCTATCTCAGA+AGG + chr2.3:12089599-12089618 MS.gene35557:CDS 45.0%
CAAAGAAGCTACTCTTGGAC+TGG - chr2.3:12090164-12090183 None:intergenic 45.0%
CATTCTGTCCTTTCACCCTT+AGG - chr2.3:12089295-12089314 None:intergenic 45.0%
CTAAGGGTGAAAGGACAGAA+TGG + chr2.3:12089293-12089312 MS.gene35557:CDS 45.0%
TCTGGCAAAGAAGCTACTCT+TGG - chr2.3:12090169-12090188 None:intergenic 45.0%
TGGTTGTTTGTCGTCTCAAG+AGG + chr2.3:12089459-12089478 MS.gene35557:CDS 45.0%
TGTCTGCGAACAAGAAAAGG+AGG + chr2.3:12089646-12089665 MS.gene35557:CDS 45.0%
! AAAGAGCGCAACGTGAAATC+GGG + chr2.3:12089217-12089236 MS.gene35557:CDS 45.0%
!! GCGGTACTGATCATAGAGAA+AGG + chr2.3:12089621-12089640 MS.gene35557:CDS 45.0%
!! GGTTGATGGTAGTTCCAAAC+GGG + chr2.3:12089667-12089686 MS.gene35557:CDS 45.0%
!! GTTGATGGTAGTTCCAAACG+GGG + chr2.3:12089668-12089687 MS.gene35557:CDS 45.0%
!! GTTTTTGAGAAACGAGCTCC+TGG - chr2.3:12090135-12090154 None:intergenic 45.0%
AAACGGGGAAGCAGCAGTTA+TGG + chr2.3:12089683-12089702 MS.gene35557:CDS 50.0%
CACTTAGGTCGTGCACCTAA+GGG + chr2.3:12089277-12089296 MS.gene35557:CDS 50.0%
GACTGTGCTATCTCAGAAGG+CGG + chr2.3:12089602-12089621 MS.gene35557:CDS 50.0%
GCACATTCTCATCCATGCCA+AGG + chr2.3:12090057-12090076 MS.gene35557:CDS 50.0%
GCTCCTGGTTGAGAATGTGT+TGG - chr2.3:12090120-12090139 None:intergenic 50.0%
GGCATGGATGAGAATGTGCA+AGG - chr2.3:12090056-12090075 None:intergenic 50.0%
GGGTGTCTGCGAACAAGAAA+AGG + chr2.3:12089643-12089662 MS.gene35557:CDS 50.0%
TCACTTAGGTCGTGCACCTA+AGG + chr2.3:12089276-12089295 MS.gene35557:CDS 50.0%
TGTGGCTATTGGAAAGCCAC+TGG + chr2.3:12089193-12089212 MS.gene35557:CDS 50.0%
! ACGAGTACTGTGTCAGTGAC+AGG + chr2.3:12089323-12089342 MS.gene35557:CDS 50.0%
! ATAACTGCTGCTTCCCCGTT+TGG - chr2.3:12089684-12089703 None:intergenic 50.0%
! GAGCTACTGACTGTGGCTAT+TGG + chr2.3:12089182-12089201 MS.gene35557:CDS 50.0%
AGACTCTTCGGGCCAGCTAA+AGG + chr2.3:12089778-12089797 MS.gene35557:CDS 55.0%
CTGTGTCAGTGACAGGTCTC+CGG + chr2.3:12089330-12089349 MS.gene35557:CDS 55.0%
GTCGTGCACCTAAGGGTGAA+AGG + chr2.3:12089284-12089303 MS.gene35557:CDS 55.0%
TGGTTCTTCTTCTCTCCCCG+TGG + chr2.3:12089127-12089146 MS.gene35557:CDS 55.0%
TTCTCCGTTGTGCAGTGCCT+TGG - chr2.3:12090077-12090096 None:intergenic 55.0%
! CACGTTGCGCTCTTTTCCAG+TGG - chr2.3:12089212-12089231 None:intergenic 55.0%
!! CATGCCAAGGCACTGCACAA+CGG + chr2.3:12090070-12090089 MS.gene35557:CDS 55.0%
CGTTGTGCAGTGCCTTGGCA+TGG - chr2.3:12090072-12090091 None:intergenic 60.0%
GTCAGTGACAGGTCTCCGGT+GGG + chr2.3:12089334-12089353 MS.gene35557:intron 60.0%
TGTCAGTGACAGGTCTCCGG+TGG + chr2.3:12089333-12089352 MS.gene35557:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 12089098 12090304 12089098 ID=MS.gene35557
chr2.3 mRNA 12089098 12090304 12089098 ID=MS.gene35557.t1;Parent=MS.gene35557
chr2.3 exon 12089098 12089351 12089098 ID=MS.gene35557.t1.exon1;Parent=MS.gene35557.t1
chr2.3 CDS 12089098 12089351 12089098 ID=cds.MS.gene35557.t1;Parent=MS.gene35557.t1
chr2.3 exon 12089452 12089799 12089452 ID=MS.gene35557.t1.exon2;Parent=MS.gene35557.t1
chr2.3 CDS 12089452 12089799 12089452 ID=cds.MS.gene35557.t1;Parent=MS.gene35557.t1
chr2.3 exon 12089955 12090304 12089955 ID=MS.gene35557.t1.exon3;Parent=MS.gene35557.t1
chr2.3 CDS 12089955 12090304 12089955 ID=cds.MS.gene35557.t1;Parent=MS.gene35557.t1
Gene Sequence

>MS.gene35557

CCAAATCATTCATTCAATCAGATAACGAGTGGTTCTTCTTCTCTCCCCGTGGAAGAAAATATCCAAATGGTTCACAAAGTAAAAGAGCTACTGACTGTGGCTATTGGAAAGCCACTGGAAAAGAGCGCAACGTGAAATCGGGTTCTAATGTCATAGGTACAAAGCGAACTTTGGTGTTTCACTTAGGTCGTGCACCTAAGGGTGAAAGGACAGAATGGATTATGCACGAGTACTGTGTCAGTGACAGGTCTCCGGTGGGTAATAATCTGAATGTATCCTTCCTCTCCCTATGAATGTTAAAAAACAAGAGTGACTCATAAATTATTTGTTTGTTGTTTGTTATGTATGTTTCAGGATTCTTTGGTTGTTTGTCGTCTCAAGAGGAACACAGAGTTTCGTGTTAACGATGTTTCAAATAGAGCATCTTCAAGTCAAATAAATCCTGTACATTCACATGAAAGTGATTGTGCTGGTCAAATAAACCCAGTAAATTCACATGAAAGTGACTGTGCTATCTCAGAAGGCGGTACTGATCATAGAGAAAGGGGTGTCTGCGAACAAGAAAAGGAGGTTGATGGTAGTTCCAAACGGGGAAGCAGCAGTTATGGTTCAGCTTCAATGGAACAAATCGATTCTGTGTCTGAATCAAATCAGCGACCAGCTAATGACGGTTTTTTGACAGACTCTTCGGGCCAGCTAAAGGTATAAAATTGATTTTGAAGTTAACAGAAAAAAACTTTTGCAACCGTAGAATGAGTACATTACACGTATATTATTAGACTAGATTCAGCTTTTTGCATCGGATAAAATTGAGCATTTAATGTTACTTAGCCTTTTATGATGATATTGTTATGTAGATGGATGAAGAGGATTGTTATGCTGAGATACTAAGGAATGATATCATAAAATTAGATGAATCATCAATGTCTCAAGGAGCTCAAACAAGACCAAATAACCTTGCACATTCTCATCCATGCCAAGGCACTGCACAACGGAGAATACGATTAAGGGTTGCGAAACCAACACATTCTCAACCAGGAGCTCGTTTCTCAAAAACAAAGCACCAGTCCAAGAGTAGCTTCTTTGCCAGAATGACCAATCGTTTGATGATATTTGCTTTCTTCATTTTCACTCTCACGGCTATATTATTTTGGTCTTCATTTGGTGGTCACAAACAAGTTAGAAATATTGTAGGAAAATTTCAGGT

Protein sequence

>MS.gene35557.t1

PNHSFNQITSGSSSLPVEENIQMVHKVKELLTVAIGKPLEKSATUNRVLMSUVQSELWCFTUVVHLRVKGQNGLCTSTVSVTGLRILWLFVVSRGTQSFVLTMFQIEHLQVKUILYIHMKVIVLVKUTQUIHMKVTVLSQKAVLIIEKGVSANKKRRLMVVPNGEAAVMVQLQWNKSILCLNQISDQLMTVFUQTLRASURWMKRIVMLRYUGMISUNUMNHQCLKELKQDQITLHILIHAKALHNGEYDUGLRNQHILNQELVSQKQSTSPRVASLPEUPIVUUYLLSSFSLSRLYYFGLHLVVTNKLEILUENFR