Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36061.t1 | AET02997.1 | 98.4 | 63 | 1 | 0 | 1 | 63 | 1 | 63 | 2.10E-29 | 137.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36061.t1 | Q96386 | 66.7 | 66 | 18 | 3 | 1 | 63 | 1 | 65 | 4.2e-18 | 91.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36061.t1 | G7LCX2 | 98.4 | 63 | 1 | 0 | 1 | 63 | 1 | 63 | 1.5e-29 | 137.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049149 | MS.gene36061 | 0.817875 | 2.63E-52 | -1.69E-46 |
| MS.gene049150 | MS.gene36061 | 0.819453 | 1.15E-52 | -1.69E-46 |
| MS.gene049153 | MS.gene36061 | 0.807469 | 4.98E-50 | -1.69E-46 |
| MS.gene049260 | MS.gene36061 | 0.804754 | 1.86E-49 | -1.69E-46 |
| MS.gene050680 | MS.gene36061 | 0.845918 | 3.01E-59 | -1.69E-46 |
| MS.gene050750 | MS.gene36061 | 0.832175 | 1.10E-55 | -1.69E-46 |
| MS.gene050820 | MS.gene36061 | 0.812551 | 4.01E-51 | -1.69E-46 |
| MS.gene050953 | MS.gene36061 | 0.845052 | 5.17E-59 | -1.69E-46 |
| MS.gene05119 | MS.gene36061 | -0.817108 | 3.91E-52 | -1.69E-46 |
| MS.gene052426 | MS.gene36061 | 0.829137 | 6.09E-55 | -1.69E-46 |
| MS.gene053166 | MS.gene36061 | 0.80202 | 6.84E-49 | -1.69E-46 |
| MS.gene053494 | MS.gene36061 | 0.862624 | 4.44E-64 | -1.69E-46 |
| MS.gene053555 | MS.gene36061 | 0.801731 | 7.84E-49 | -1.69E-46 |
| MS.gene053584 | MS.gene36061 | 0.806224 | 9.13E-50 | -1.69E-46 |
| MS.gene054405 | MS.gene36061 | 0.844991 | 5.36E-59 | -1.69E-46 |
| MS.gene054542 | MS.gene36061 | 0.81609 | 6.62E-52 | -1.69E-46 |
| MS.gene054559 | MS.gene36061 | 0.805096 | 1.57E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene36061 | 0.823885 | 1.09E-53 | -1.69E-46 |
| MS.gene05510 | MS.gene36061 | 0.831166 | 1.95E-55 | -1.69E-46 |
| MS.gene055356 | MS.gene36061 | 0.817393 | 3.38E-52 | -1.69E-46 |
| MS.gene055358 | MS.gene36061 | 0.815814 | 7.62E-52 | -1.69E-46 |
| MS.gene055701 | MS.gene36061 | 0.844133 | 9.13E-59 | -1.69E-46 |
| MS.gene056723 | MS.gene36061 | 0.809194 | 2.13E-50 | -1.69E-46 |
| MS.gene057194 | MS.gene36061 | 0.801465 | 8.89E-49 | -1.69E-46 |
| MS.gene057394 | MS.gene36061 | 0.80751 | 4.88E-50 | -1.69E-46 |
| MS.gene057639 | MS.gene36061 | 0.837106 | 6.32E-57 | -1.69E-46 |
| MS.gene059128 | MS.gene36061 | 0.826437 | 2.71E-54 | -1.69E-46 |
| MS.gene059161 | MS.gene36061 | 0.822805 | 1.95E-53 | -1.69E-46 |
| MS.gene059285 | MS.gene36061 | 0.803786 | 2.95E-49 | -1.69E-46 |
| MS.gene059609 | MS.gene36061 | 0.823958 | 1.05E-53 | -1.69E-46 |
| MS.gene060322 | MS.gene36061 | 0.842737 | 2.15E-58 | -1.69E-46 |
| MS.gene060998 | MS.gene36061 | 0.805534 | 1.27E-49 | -1.69E-46 |
| MS.gene061002 | MS.gene36061 | 0.815214 | 1.04E-51 | -1.69E-46 |
| MS.gene061114 | MS.gene36061 | -0.848816 | 4.82E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36061.t1 | MTR_8g060850 | 98.413 | 63 | 1 | 0 | 1 | 63 | 1 | 63 | 3.86e-40 | 125 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36061.t1 | AT3G15353 | 57.576 | 66 | 23 | 2 | 1 | 61 | 1 | 66 | 2.95e-17 | 68.6 |
| MS.gene36061.t1 | AT3G15353 | 56.923 | 65 | 23 | 2 | 1 | 61 | 1 | 64 | 1.59e-16 | 66.6 |
Find 12 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCAATCATGCTCAACAGC+TGG | 0.507300 | 8.1:-52803273 | None:intergenic |
| ATATATTCTTTAATGACCGC+AGG | 0.551610 | 8.1:-52803328 | None:intergenic |
| AGAGGAGCACTTACCCGCAC+TGG | 0.574771 | 8.1:-52802660 | None:intergenic |
| GTTGAGCATGATTGCAAGTG+TGG | 0.581040 | 8.1:+52803279 | MS.gene36061:CDS |
| CTTGGTCGAGACTGTTGTCA+TGG | 0.585178 | 8.1:+52803245 | MS.gene36061:CDS |
| TTCAGGAAGGGAAACAACTA+CGG | 0.588697 | 8.1:+52803081 | MS.gene36061:intron |
| GCGCTGACAAGAGCCAGTGC+GGG | 0.597634 | 8.1:+52802647 | MS.gene36061:CDS |
| ACAAACATGTCAAGCTCATG+CGG | 0.602184 | 8.1:+52802613 | None:intergenic |
| TGCGCTGACAAGAGCCAGTG+CGG | 0.611618 | 8.1:+52802646 | MS.gene36061:CDS |
| ACTTGCACTAACTGCACCTG+CGG | 0.677689 | 8.1:+52803312 | MS.gene36061:CDS |
| CCTCTTCTGAGTCTCGACAA+TGG | 0.679042 | 8.1:-52803109 | None:intergenic |
| CCATTGTCGAGACTCAGAAG+AGG | 0.690774 | 8.1:+52803109 | MS.gene36061:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAACACTTATGATATAAG+TGG | + | chr8.1:52802827-52802846 | MS.gene36061:intron | 20.0% |
| !! | GAGTTTATAAAAATAGCTTA+TGG | - | chr8.1:52802948-52802967 | None:intergenic | 20.0% |
| ! | TTTAGAAAAACGGCTTAATT+AGG | + | chr8.1:52802794-52802813 | MS.gene36061:intron | 25.0% |
| AAGGTTAATGTTGTACTGTA+AGG | + | chr8.1:52802695-52802714 | MS.gene36061:intron | 30.0% | |
| ATACCATAGCATCATATATG+AGG | - | chr8.1:52803062-52803081 | None:intergenic | 30.0% | |
| TACCATAGCATCATATATGA+GGG | - | chr8.1:52803061-52803080 | None:intergenic | 30.0% | |
| TATATGATGCTATGGTATTC+AGG | + | chr8.1:52803064-52803083 | MS.gene36061:intron | 30.0% | |
| ! | TTGTTTTCATAAGCTGAAGT+CGG | + | chr8.1:52802910-52802929 | MS.gene36061:intron | 30.0% |
| !!! | TTAAGCCGTTTTTCTAAACA+AGG | - | chr8.1:52802792-52802811 | None:intergenic | 30.0% |
| AACCTTACAGTACAAGATAG+AGG | - | chr8.1:52802681-52802700 | None:intergenic | 35.0% | |
| ACCATAGCATCATATATGAG+GGG | - | chr8.1:52803060-52803079 | None:intergenic | 35.0% | |
| CAGCTTATGAAAACAACTTC+AGG | - | chr8.1:52802906-52802925 | None:intergenic | 35.0% | |
| GCGTTCACTCATTTATATAG+AGG | + | chr8.1:52802766-52802785 | MS.gene36061:intron | 35.0% | |
| TCGAGGAGTTATTAGAAACA+TGG | - | chr8.1:52803164-52803183 | None:intergenic | 35.0% | |
| !! | AGAGGCCTTGTTTAGAAAAA+CGG | + | chr8.1:52802784-52802803 | MS.gene36061:intron | 35.0% |
| CTCCTCTATCTTGTACTGTA+AGG | + | chr8.1:52802676-52802695 | MS.gene36061:intron | 40.0% | |
| GATGCTATGGTATTCAGGAA+GGG | + | chr8.1:52803069-52803088 | MS.gene36061:intron | 40.0% | |
| TCCCCTCATATATGATGCTA+TGG | + | chr8.1:52803056-52803075 | MS.gene36061:intron | 40.0% | |
| TCTCACAAACAAATCCGTAC+AGG | + | chr8.1:52802978-52802997 | MS.gene36061:intron | 40.0% | |
| TGATGCTATGGTATTCAGGA+AGG | + | chr8.1:52803068-52803087 | MS.gene36061:intron | 40.0% | |
| TTCAGGAAGGGAAACAACTA+CGG | + | chr8.1:52803081-52803100 | MS.gene36061:intron | 40.0% | |
| ! | GTCAAATGTTTTGTGCAGCT+TGG | + | chr8.1:52803227-52803246 | MS.gene36061:intron | 40.0% |
| ! | TACGGATTTGTTTGTGAGAC+TGG | - | chr8.1:52802977-52802996 | None:intergenic | 40.0% |
| AACAAATCCGTACAGGCTTG+TGG | + | chr8.1:52802985-52803004 | MS.gene36061:intron | 45.0% | |
| GTTGAGCATGATTGCAAGTG+TGG | + | chr8.1:52803279-52803298 | MS.gene36061:CDS | 45.0% | |
| TTGCAATCATGCTCAACAGC+TGG | - | chr8.1:52803276-52803295 | None:intergenic | 45.0% | |
| ! | CATAAGCTGAAGTCGGCTTA+TGG | + | chr8.1:52802917-52802936 | MS.gene36061:intron | 45.0% |
| !! | CTAGCTTTTGCATTGAGTCG+AGG | - | chr8.1:52803181-52803200 | None:intergenic | 45.0% |
| !!! | TATTTCTATAAATAAAATAA+AGG | + | chr8.1:52802862-52802881 | MS.gene36061:intron | 5.0% |
| ACTTGCACTAACTGCACCTG+CGG | + | chr8.1:52803312-52803331 | MS.gene36061:CDS | 50.0% | |
| CCATTGTCGAGACTCAGAAG+AGG | + | chr8.1:52803109-52803128 | MS.gene36061:CDS | 50.0% | |
| CCTCTTCTGAGTCTCGACAA+TGG | - | chr8.1:52803112-52803131 | None:intergenic | 50.0% | |
| CTACAAGCCACAAGCCTGTA+CGG | - | chr8.1:52802995-52803014 | None:intergenic | 50.0% | |
| CTTGGTCGAGACTGTTGTCA+TGG | + | chr8.1:52803245-52803264 | MS.gene36061:CDS | 50.0% | |
| ! | TGCGCTGACAAGAGCCAGTG+CGG | + | chr8.1:52802646-52802665 | MS.gene36061:CDS | 60.0% |
| !! | AGAGGAGCACTTACCCGCAC+TGG | - | chr8.1:52802663-52802682 | None:intergenic | 60.0% |
| ! | GCGCTGACAAGAGCCAGTGC+GGG | + | chr8.1:52802647-52802666 | MS.gene36061:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 52802619 | 52803341 | 52802619 | ID=MS.gene36061 |
| chr8.1 | mRNA | 52802619 | 52803341 | 52802619 | ID=MS.gene36061.t1;Parent=MS.gene36061 |
| chr8.1 | exon | 52802619 | 52802668 | 52802619 | ID=MS.gene36061.t1.exon1;Parent=MS.gene36061.t1 |
| chr8.1 | CDS | 52802619 | 52802668 | 52802619 | ID=cds.MS.gene36061.t1;Parent=MS.gene36061.t1 |
| chr8.1 | exon | 52803086 | 52803130 | 52803086 | ID=MS.gene36061.t1.exon2;Parent=MS.gene36061.t1 |
| chr8.1 | CDS | 52803086 | 52803130 | 52803086 | ID=cds.MS.gene36061.t1;Parent=MS.gene36061.t1 |
| chr8.1 | exon | 52803245 | 52803341 | 52803245 | ID=MS.gene36061.t1.exon3;Parent=MS.gene36061.t1 |
| chr8.1 | CDS | 52803245 | 52803341 | 52803245 | ID=cds.MS.gene36061.t1;Parent=MS.gene36061.t1 |
| Gene Sequence |
| Protein sequence |
