Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36497.t1 | XP_003621678.1 | 94 | 168 | 10 | 0 | 1 | 168 | 153 | 320 | 2.10E-76 | 295 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36497.t1 | A2Q573 | 94.0 | 168 | 10 | 0 | 1 | 168 | 153 | 320 | 1.5e-76 | 295.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene36497 | 0.804635 | 1.97E-49 | -1.69E-46 |
| MS.gene049680 | MS.gene36497 | 0.833893 | 4.10E-56 | -1.69E-46 |
| MS.gene050414 | MS.gene36497 | 0.824051 | 9.95E-54 | -1.69E-46 |
| MS.gene050752 | MS.gene36497 | 0.835494 | 1.62E-56 | -1.69E-46 |
| MS.gene050945 | MS.gene36497 | 0.835082 | 2.06E-56 | -1.69E-46 |
| MS.gene050955 | MS.gene36497 | 0.843733 | 1.17E-58 | -1.69E-46 |
| MS.gene051003 | MS.gene36497 | 0.838047 | 3.63E-57 | -1.69E-46 |
| MS.gene051043 | MS.gene36497 | 0.86003 | 2.74E-63 | -1.69E-46 |
| MS.gene051234 | MS.gene36497 | 0.851103 | 1.10E-60 | -1.69E-46 |
| MS.gene051699 | MS.gene36497 | 0.817584 | 3.06E-52 | -1.69E-46 |
| MS.gene051902 | MS.gene36497 | 0.80145 | 8.95E-49 | -1.69E-46 |
| MS.gene052296 | MS.gene36497 | 0.850602 | 1.53E-60 | -1.69E-46 |
| MS.gene052335 | MS.gene36497 | 0.811608 | 6.43E-51 | -1.69E-46 |
| MS.gene052533 | MS.gene36497 | 0.819176 | 1.33E-52 | -1.69E-46 |
| MS.gene052793 | MS.gene36497 | 0.848551 | 5.70E-60 | -1.69E-46 |
| MS.gene053298 | MS.gene36497 | 0.803848 | 2.87E-49 | -1.69E-46 |
| MS.gene053879 | MS.gene36497 | 0.831124 | 1.99E-55 | -1.69E-46 |
| MS.gene054403 | MS.gene36497 | 0.84248 | 2.52E-58 | -1.69E-46 |
| MS.gene05469 | MS.gene36497 | 0.816358 | 5.76E-52 | -1.69E-46 |
| MS.gene055555 | MS.gene36497 | 0.807352 | 5.27E-50 | -1.69E-46 |
| MS.gene056082 | MS.gene36497 | 0.802723 | 4.90E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene36497 | 0.844936 | 5.55E-59 | -1.69E-46 |
| MS.gene058159 | MS.gene36497 | 0.82517 | 5.42E-54 | -1.69E-46 |
| MS.gene058261 | MS.gene36497 | 0.81419 | 1.75E-51 | -1.69E-46 |
| MS.gene059146 | MS.gene36497 | 0.825082 | 5.69E-54 | -1.69E-46 |
| MS.gene05985 | MS.gene36497 | -0.836035 | 1.18E-56 | -1.69E-46 |
| MS.gene060259 | MS.gene36497 | 0.803963 | 2.71E-49 | -1.69E-46 |
| MS.gene061100 | MS.gene36497 | 0.832867 | 7.40E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36497.t1 | MTR_7g021620 | 97.619 | 168 | 4 | 0 | 1 | 168 | 153 | 320 | 3.06e-114 | 327 |
| MS.gene36497.t1 | MTR_4g096890 | 95.833 | 168 | 7 | 0 | 1 | 168 | 153 | 320 | 5.08e-113 | 323 |
| MS.gene36497.t1 | MTR_3g014430 | 67.961 | 103 | 22 | 3 | 51 | 148 | 16 | 112 | 3.17e-34 | 117 |
| MS.gene36497.t1 | MTR_1g029440 | 43.373 | 166 | 89 | 2 | 1 | 165 | 169 | 330 | 1.27e-29 | 110 |
| MS.gene36497.t1 | MTR_3g088975 | 44.667 | 150 | 81 | 2 | 17 | 165 | 181 | 329 | 2.08e-29 | 110 |
| MS.gene36497.t1 | MTR_3g088965 | 39.007 | 141 | 83 | 2 | 18 | 155 | 184 | 324 | 8.90e-20 | 84.3 |
| MS.gene36497.t1 | MTR_3g014620 | 73.333 | 45 | 12 | 0 | 12 | 56 | 12 | 56 | 2.35e-13 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36497.t1 | AT4G19950 | 76.190 | 168 | 40 | 0 | 1 | 168 | 154 | 321 | 5.51e-80 | 240 |
| MS.gene36497.t1 | AT5G44860 | 72.619 | 168 | 46 | 0 | 1 | 168 | 154 | 321 | 1.89e-77 | 233 |
| MS.gene36497.t1 | AT5G44860 | 72.619 | 168 | 46 | 0 | 1 | 168 | 154 | 321 | 1.89e-77 | 233 |
| MS.gene36497.t1 | AT1G31130 | 64.286 | 168 | 60 | 0 | 1 | 168 | 154 | 321 | 3.45e-60 | 189 |
Find 33 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCATTCAAATGGAAAATT+TGG | 0.141896 | 4.1:-18200748 | MS.gene36497:CDS |
| TGTTCTTGTCATTGTGAATT+TGG | 0.199328 | 4.1:-18200895 | MS.gene36497:CDS |
| TCTGTGCTTGAACCTCTTTA+TGG | 0.244172 | 4.1:-18201101 | MS.gene36497:CDS |
| GATTGTCATTGGTGGGTTCT+TGG | 0.268660 | 4.1:-18200922 | MS.gene36497:CDS |
| GGTTAGATATGCTGCACTTT+TGG | 0.290596 | 4.1:-18201036 | MS.gene36497:CDS |
| TGTGAATTTGGTTGGGTTGT+TGG | 0.309528 | 4.1:-18200883 | MS.gene36497:CDS |
| TATAACTGCTTTGTGGCATT+TGG | 0.326265 | 4.1:-18201135 | MS.gene36497:CDS |
| CTTGTCATTGTGAATTTGGT+TGG | 0.385554 | 4.1:-18200891 | MS.gene36497:CDS |
| ATTTGAATGCTGCTCTTAAG+AGG | 0.385789 | 4.1:+18200759 | None:intergenic |
| CTTATTTGCGCGGTTATTAG+TGG | 0.393312 | 4.1:-18200999 | MS.gene36497:CDS |
| TTTCTAGGATTGTCATTGGT+GGG | 0.414842 | 4.1:-18200929 | MS.gene36497:CDS |
| GAAGAGTTATGAGTTGTTGA+AGG | 0.420597 | 4.1:-18201063 | MS.gene36497:CDS |
| ATGTTTATATAACTGCTTTG+TGG | 0.423556 | 4.1:-18201142 | MS.gene36497:CDS |
| TTGTCATTGTGAATTTGGTT+GGG | 0.428320 | 4.1:-18200890 | MS.gene36497:CDS |
| TTATGAGTTGTTGAAGGGTA+GGG | 0.428981 | 4.1:-18201057 | MS.gene36497:CDS |
| GTTATGAGTTGTTGAAGGGT+AGG | 0.441707 | 4.1:-18201058 | MS.gene36497:CDS |
| GTCATTGGTGGGTTCTTGGT+TGG | 0.447415 | 4.1:-18200918 | MS.gene36497:CDS |
| TTTAGCGTGGTTGTGGTACA+TGG | 0.449111 | 4.1:-18200972 | MS.gene36497:CDS |
| GTACATGGTGGTGATGGTTA+TGG | 0.461928 | 4.1:-18200957 | MS.gene36497:CDS |
| GATCATCTTGGTGGCTACCT+TGG | 0.466274 | 4.1:-18200792 | MS.gene36497:CDS |
| TCTTAAGAGCAGCATTCAAA+TGG | 0.471769 | 4.1:-18200757 | MS.gene36497:CDS |
| TGGGATGTTTAGCGTGGTTG+TGG | 0.508402 | 4.1:-18200979 | MS.gene36497:CDS |
| AAGAGTTATGAGTTGTTGAA+GGG | 0.511629 | 4.1:-18201062 | MS.gene36497:CDS |
| AGTGCTTTACATGATCATCT+TGG | 0.515132 | 4.1:-18200804 | MS.gene36497:CDS |
| TTATTTGCGCGGTTATTAGT+GGG | 0.540153 | 4.1:-18200998 | MS.gene36497:CDS |
| GCTTTACATGATCATCTTGG+TGG | 0.540813 | 4.1:-18200801 | MS.gene36497:CDS |
| ATAGCAGCAAAACCATAAAG+AGG | 0.564333 | 4.1:+18201089 | None:intergenic |
| GTTGTGGTACATGGTGGTGA+TGG | 0.590598 | 4.1:-18200963 | MS.gene36497:CDS |
| CGGGTATTTGCTTATTTGCG+CGG | 0.599347 | 4.1:-18201009 | MS.gene36497:CDS |
| TAAGAGGCACATATTCTCCA+AGG | 0.610436 | 4.1:+18200775 | None:intergenic |
| AGCAGTTGATACTGACAACT+CGG | 0.612984 | 4.1:-18201216 | MS.gene36497:CDS |
| TATTAGTGGGATGTTTAGCG+TGG | 0.653236 | 4.1:-18200985 | MS.gene36497:CDS |
| AGCGTGGTTGTGGTACATGG+TGG | 0.722658 | 4.1:-18200969 | MS.gene36497:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGTTTATATAACTGCTTTG+TGG | - | chr4.1:18200822-18200841 | MS.gene36497:CDS | 25.0% |
| AAGAGTTATGAGTTGTTGAA+GGG | - | chr4.1:18200902-18200921 | MS.gene36497:CDS | 30.0% | |
| CAGCATTCAAATGGAAAATT+TGG | - | chr4.1:18201216-18201235 | MS.gene36497:CDS | 30.0% | |
| TGTTCTTGTCATTGTGAATT+TGG | - | chr4.1:18201069-18201088 | MS.gene36497:CDS | 30.0% | |
| TTGTCATTGTGAATTTGGTT+GGG | - | chr4.1:18201074-18201093 | MS.gene36497:CDS | 30.0% | |
| AGTGCTTTACATGATCATCT+TGG | - | chr4.1:18201160-18201179 | MS.gene36497:CDS | 35.0% | |
| ATAGCAGCAAAACCATAAAG+AGG | + | chr4.1:18200878-18200897 | None:intergenic | 35.0% | |
| ATTTGAATGCTGCTCTTAAG+AGG | + | chr4.1:18201208-18201227 | None:intergenic | 35.0% | |
| CTTGTCATTGTGAATTTGGT+TGG | - | chr4.1:18201073-18201092 | MS.gene36497:CDS | 35.0% | |
| GAAGAGTTATGAGTTGTTGA+AGG | - | chr4.1:18200901-18200920 | MS.gene36497:CDS | 35.0% | |
| TATAACTGCTTTGTGGCATT+TGG | - | chr4.1:18200829-18200848 | MS.gene36497:CDS | 35.0% | |
| TCTTAAGAGCAGCATTCAAA+TGG | - | chr4.1:18201207-18201226 | MS.gene36497:CDS | 35.0% | |
| TTATGAGTTGTTGAAGGGTA+GGG | - | chr4.1:18200907-18200926 | MS.gene36497:CDS | 35.0% | |
| TTATTTGCGCGGTTATTAGT+GGG | - | chr4.1:18200966-18200985 | MS.gene36497:CDS | 35.0% | |
| !! | TTTCTAGGATTGTCATTGGT+GGG | - | chr4.1:18201035-18201054 | MS.gene36497:CDS | 35.0% |
| !!! | GTGTTTTCTAGGATTGTCAT+TGG | - | chr4.1:18201031-18201050 | MS.gene36497:CDS | 35.0% |
| !!! | TTTTCTAGGATTGTCATTGG+TGG | - | chr4.1:18201034-18201053 | MS.gene36497:CDS | 35.0% |
| CTTATTTGCGCGGTTATTAG+TGG | - | chr4.1:18200965-18200984 | MS.gene36497:CDS | 40.0% | |
| GCTTTACATGATCATCTTGG+TGG | - | chr4.1:18201163-18201182 | MS.gene36497:CDS | 40.0% | |
| GTTATGAGTTGTTGAAGGGT+AGG | - | chr4.1:18200906-18200925 | MS.gene36497:CDS | 40.0% | |
| TATTAGTGGGATGTTTAGCG+TGG | - | chr4.1:18200979-18200998 | MS.gene36497:CDS | 40.0% | |
| TCTGTGCTTGAACCTCTTTA+TGG | - | chr4.1:18200863-18200882 | MS.gene36497:CDS | 40.0% | |
| ! | AGCAGTTGATACTGACAACT+CGG | - | chr4.1:18200748-18200767 | MS.gene36497:CDS | 40.0% |
| ! | ATGGTTATGGCGTGTTTTCT+AGG | - | chr4.1:18201020-18201039 | MS.gene36497:CDS | 40.0% |
| ! | GGTTAGATATGCTGCACTTT+TGG | - | chr4.1:18200928-18200947 | MS.gene36497:CDS | 40.0% |
| ! | TAAGAGGCACATATTCTCCA+AGG | + | chr4.1:18201192-18201211 | None:intergenic | 40.0% |
| ! | TGTGAATTTGGTTGGGTTGT+TGG | - | chr4.1:18201081-18201100 | MS.gene36497:CDS | 40.0% |
| !!! | TATGCTGCACTTTTGGTTTG+CGG | - | chr4.1:18200935-18200954 | MS.gene36497:CDS | 40.0% |
| CGGGTATTTGCTTATTTGCG+CGG | - | chr4.1:18200955-18200974 | MS.gene36497:CDS | 45.0% | |
| TTTAGCGTGGTTGTGGTACA+TGG | - | chr4.1:18200992-18201011 | MS.gene36497:CDS | 45.0% | |
| !! | GATTGTCATTGGTGGGTTCT+TGG | - | chr4.1:18201042-18201061 | MS.gene36497:CDS | 45.0% |
| !! | GTACATGGTGGTGATGGTTA+TGG | - | chr4.1:18201007-18201026 | MS.gene36497:CDS | 45.0% |
| !!! | ATGCTGCACTTTTGGTTTGC+GGG | - | chr4.1:18200936-18200955 | MS.gene36497:CDS | 45.0% |
| TGGGATGTTTAGCGTGGTTG+TGG | - | chr4.1:18200985-18201004 | MS.gene36497:CDS | 50.0% | |
| ! | GATCATCTTGGTGGCTACCT+TGG | - | chr4.1:18201172-18201191 | MS.gene36497:CDS | 50.0% |
| ! | GTTGTGGTACATGGTGGTGA+TGG | - | chr4.1:18201001-18201020 | MS.gene36497:CDS | 50.0% |
| !! | GTCATTGGTGGGTTCTTGGT+TGG | - | chr4.1:18201046-18201065 | MS.gene36497:CDS | 50.0% |
| AGCGTGGTTGTGGTACATGG+TGG | - | chr4.1:18200995-18201014 | MS.gene36497:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 18200740 | 18201246 | 18200740 | ID=MS.gene36497 |
| chr4.1 | mRNA | 18200740 | 18201246 | 18200740 | ID=MS.gene36497.t1;Parent=MS.gene36497 |
| chr4.1 | exon | 18200740 | 18201246 | 18200740 | ID=MS.gene36497.t1.exon1;Parent=MS.gene36497.t1 |
| chr4.1 | CDS | 18200740 | 18201246 | 18200740 | ID=cds.MS.gene36497.t1;Parent=MS.gene36497.t1 |
| Gene Sequence |
| Protein sequence |
