AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39895

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39895.t1 MTR_1g070385 95.918 294 12 0 1 294 1 294 0.0 582
MS.gene39895.t1 MTR_1g097840 43.556 225 127 0 57 281 69 293 5.60e-56 183
MS.gene39895.t1 MTR_2g017590 41.256 223 130 1 62 283 36 258 3.32e-54 177
MS.gene39895.t1 MTR_7g010650 38.136 236 139 1 47 275 15 250 2.35e-53 175
MS.gene39895.t1 MTR_2g104940 40.175 229 137 0 60 288 78 306 1.25e-52 174
MS.gene39895.t1 MTR_2g017620 42.396 217 124 1 60 275 22 238 1.96e-52 172
MS.gene39895.t1 MTR_2g017620 42.396 217 124 1 60 275 36 252 2.07e-52 172
MS.gene39895.t1 MTR_2g017660 39.224 232 139 2 62 291 39 270 2.17e-51 170
MS.gene39895.t1 MTR_8g087720 38.182 220 135 1 59 277 35 254 4.90e-51 169
MS.gene39895.t1 MTR_8g087710 40.826 218 128 1 60 276 37 254 4.68e-50 167
MS.gene39895.t1 MTR_4g006730 40.909 220 130 0 60 279 73 292 8.50e-50 167
MS.gene39895.t1 MTR_4g084880 36.444 225 140 2 62 283 35 259 2.15e-49 165
MS.gene39895.t1 MTR_2g017610 38.565 223 136 1 62 283 38 260 5.36e-49 164
MS.gene39895.t1 MTR_2g017570 38.117 223 137 1 62 283 38 260 9.37e-49 163
MS.gene39895.t1 MTR_5g079570 37.727 220 135 2 58 276 36 254 2.10e-45 154
MS.gene39895.t1 MTR_4g006630 42.473 186 107 0 94 279 20 205 6.57e-45 152
MS.gene39895.t1 MTR_3g008920 37.662 231 142 2 50 279 14 243 9.18e-45 153
MS.gene39895.t1 MTR_4g006730 42.473 186 107 0 94 279 20 205 1.14e-44 151
MS.gene39895.t1 MTR_5g063930 36.681 229 137 4 62 285 37 262 2.54e-44 152
MS.gene39895.t1 MTR_2g017620 43.860 171 95 1 106 275 1 171 4.19e-44 149
MS.gene39895.t1 MTR_1g097840 42.857 168 96 0 57 224 69 236 7.35e-32 119
MS.gene39895.t1 MTR_4g006730 36.410 195 112 1 60 254 73 255 3.52e-30 115
MS.gene39895.t1 MTR_7g010650 35.758 165 99 1 47 204 15 179 3.71e-28 107
MS.gene39895.t1 MTR_8g087720 34.940 166 107 1 60 224 36 201 1.34e-27 106
MS.gene39895.t1 MTR_8g087720 35.616 146 93 1 60 204 36 181 3.11e-23 94.7
MS.gene39895.t1 MTR_8g013680 33.197 244 141 8 54 279 7 246 1.11e-22 94.7
MS.gene39895.t1 MTR_2g101370 32.645 242 145 8 54 279 8 247 3.86e-22 93.2
MS.gene39895.t1 MTR_1g006590 31.193 218 143 5 59 270 16 232 2.24e-20 88.2
MS.gene39895.t1 MTR_1g077540 27.119 236 161 4 62 289 21 253 8.98e-20 87.0
MS.gene39895.t1 MTR_6g069040 28.700 223 151 4 62 276 21 243 7.47e-19 84.3
MS.gene39895.t1 MTR_1g006490 30.769 221 144 6 62 275 19 237 1.07e-18 83.6
MS.gene39895.t1 MTR_5g012810 38.065 155 89 4 114 263 76 228 3.21e-18 82.4
MS.gene39895.t1 MTR_7g103030 30.396 227 149 5 65 283 24 249 1.06e-17 80.9
MS.gene39895.t1 MTR_4g063090 32.278 158 104 2 114 268 78 235 5.09e-17 79.3
MS.gene39895.t1 MTR_8g088310 76.087 46 11 0 159 204 83 128 1.37e-15 72.4
MS.gene39895.t1 MTR_3g070210 26.230 244 154 7 59 281 52 290 7.80e-15 73.6
MS.gene39895.t1 MTR_4g006650 36.232 138 79 1 66 203 8 136 1.52e-14 73.2
MS.gene39895.t1 MTR_2g060360 28.000 225 153 5 59 275 16 239 2.44e-14 71.6
MS.gene39895.t1 MTR_1g115405 26.840 231 158 5 62 281 21 251 3.07e-14 71.2
MS.gene39895.t1 MTR_2g094270 26.724 232 147 5 62 270 40 271 3.14e-14 71.6
MS.gene39895.t1 MTR_7g023350 27.706 231 156 5 62 281 21 251 4.04e-14 70.9
MS.gene39895.t1 MTR_3g118010 25.847 236 143 7 59 270 34 261 4.39e-14 71.2
MS.gene39895.t1 MTR_5g082070 26.667 225 146 6 59 265 52 275 2.18e-13 69.3
MS.gene39895.t1 MTR_8g098375 25.778 225 148 6 59 265 49 272 2.29e-13 69.3
MS.gene39895.t1 MTR_3g070210 26.238 202 127 5 59 242 52 249 8.14e-13 67.8
MS.gene39895.t1 MTR_5g010150 26.222 225 147 6 59 265 49 272 8.76e-13 67.8
MS.gene39895.t1 MTR_7g101190 25.641 234 152 6 59 270 36 269 2.32e-12 66.2
MS.gene39895.t1 MTR_4g059390 24.790 238 149 6 59 270 36 269 2.98e-12 66.2
MS.gene39895.t1 MTR_4g415300 26.068 234 135 7 59 265 53 275 3.26e-12 65.9
MS.gene39895.t1 MTR_2g094270 27.363 201 126 4 62 242 40 240 1.51e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39895.t1 AT3G06100 56.078 255 105 3 27 277 11 262 1.45e-93 278
MS.gene39895.t1 AT3G06100 60.748 214 83 1 65 277 1 214 2.55e-88 263
MS.gene39895.t1 AT4G18910 39.919 248 136 2 37 276 32 274 6.85e-56 182
MS.gene39895.t1 AT4G10380 41.778 225 131 0 57 281 73 297 8.64e-55 180
MS.gene39895.t1 AT5G37820 39.815 216 129 1 62 276 43 258 1.02e-53 176
MS.gene39895.t1 AT5G37820 39.815 216 129 1 62 276 79 294 1.25e-53 177
MS.gene39895.t1 AT4G19030 40.444 225 126 1 60 276 53 277 2.05e-53 176
MS.gene39895.t1 AT1G80760 41.096 219 129 0 57 275 75 293 1.14e-51 172
MS.gene39895.t1 AT5G37810 38.073 218 134 1 60 276 41 258 2.91e-51 170
MS.gene39895.t1 AT3G06100 54.610 141 63 1 138 277 41 181 1.11e-48 160
MS.gene39895.t1 AT1G31885 37.759 241 139 2 56 285 37 277 3.88e-48 163
MS.gene39895.t1 AT2G34390 38.117 223 129 2 56 270 42 263 1.92e-44 152
MS.gene39895.t1 AT2G34390 38.636 220 126 2 59 270 3 221 1.27e-43 149
MS.gene39895.t1 AT1G31885 38.117 223 127 2 74 285 1 223 2.54e-43 149
MS.gene39895.t1 AT4G18910 36.224 196 112 2 37 224 32 222 3.73e-33 121
MS.gene39895.t1 AT4G19030 36.257 171 101 1 60 222 53 223 1.65e-30 114
MS.gene39895.t1 AT4G19030 35.948 153 90 1 60 204 53 205 1.36e-26 104
MS.gene39895.t1 AT2G34390 31.638 177 112 2 56 224 42 217 7.60e-23 94.4
MS.gene39895.t1 AT1G73190 28.070 228 151 3 62 276 24 251 5.18e-21 90.5
MS.gene39895.t1 AT2G29870 43.119 109 60 1 178 286 22 128 2.08e-20 85.5
MS.gene39895.t1 AT4G17340 38.462 156 88 4 114 263 76 229 5.47e-20 87.4
MS.gene39895.t1 AT2G34390 32.278 158 98 2 56 205 42 198 5.77e-20 86.7
MS.gene39895.t1 AT1G17810 31.875 160 106 2 114 270 86 245 4.20e-19 85.1
MS.gene39895.t1 AT5G47450 31.925 213 132 6 62 263 19 229 4.91e-19 84.7
MS.gene39895.t1 AT3G26520 30.380 237 151 7 59 284 19 252 6.78e-19 84.3
MS.gene39895.t1 AT3G16240 31.963 219 140 5 54 263 11 229 1.17e-18 83.6
MS.gene39895.t1 AT2G25810 33.796 216 120 8 62 263 19 225 2.49e-18 82.8
MS.gene39895.t1 AT2G36830 29.870 231 152 6 62 283 21 250 3.06e-17 79.7
MS.gene39895.t1 AT2G16850 25.957 235 144 6 59 270 33 260 1.40e-15 75.5
MS.gene39895.t1 AT4G35100 26.582 237 140 9 59 270 35 262 7.24e-14 70.9
MS.gene39895.t1 AT4G35100 26.582 237 140 9 59 270 35 262 7.24e-14 70.9
MS.gene39895.t1 AT4G01470 30.864 162 101 4 114 268 78 235 7.83e-14 70.1
MS.gene39895.t1 AT3G53420 26.891 238 144 6 59 270 36 269 9.61e-14 70.5
MS.gene39895.t1 AT3G53420 26.891 238 144 6 59 270 36 269 9.61e-14 70.5
MS.gene39895.t1 AT3G54820 27.615 239 141 9 59 270 35 268 1.39e-13 70.1
MS.gene39895.t1 AT5G60660 26.809 235 142 7 62 270 39 269 2.32e-13 69.3
MS.gene39895.t1 AT3G47440 28.507 221 147 3 56 267 17 235 5.74e-13 67.8
MS.gene39895.t1 AT1G31885 37.143 105 65 1 56 159 37 141 1.47e-12 64.3
MS.gene39895.t1 AT4G23400 24.373 279 181 8 7 265 4 272 2.19e-12 66.6
MS.gene39895.t1 AT1G01620 26.293 232 147 7 59 270 49 276 4.39e-12 65.9
MS.gene39895.t1 AT4G00430 27.313 227 141 7 59 265 50 272 7.60e-12 65.1
MS.gene39895.t1 AT2G37170 25.106 235 146 6 62 270 37 267 1.35e-11 64.3
MS.gene39895.t1 AT2G37180 24.893 233 149 6 62 270 37 267 1.48e-11 63.9
MS.gene39895.t1 AT2G45960 26.316 228 142 7 59 265 49 271 1.86e-11 63.9
MS.gene39895.t1 AT2G37170 25.106 235 146 6 62 270 80 310 1.87e-11 64.3
MS.gene39895.t1 AT3G61430 25.877 228 143 7 59 265 49 271 1.98e-11 63.9
MS.gene39895.t1 AT3G61430 25.877 228 143 7 59 265 49 271 1.98e-11 63.9
MS.gene39895.t1 AT2G39010 27.236 246 133 12 59 270 35 268 9.89e-11 61.6

Find 72 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTGTGCTATTATGTAAAT+TGG 0.196563 1.4:-57709825 None:intergenic
AAAGGCCAACTGCACCATTT+TGG 0.259769 1.4:-57709377 None:intergenic
GGGCTTGCTGTACTAATTAC+AGG 0.270264 1.4:+57710159 MS.gene39895:CDS
CATGGAAATTTAAGAACATT+TGG 0.291328 1.4:+57710756 MS.gene39895:CDS
ATCAATCCATCCATATCTTT+TGG 0.297657 1.4:+57709111 MS.gene39895:CDS
AAATGGTGCAGTTGGCCTTT+TGG 0.300610 1.4:+57709380 MS.gene39895:CDS
AAGGGTGTAACTCTGCCTTC+TGG 0.313532 1.4:+57709928 MS.gene39895:CDS
GTGCAGGTGGGACATTTATC+TGG 0.315213 1.4:+57710114 MS.gene39895:intron
GACCAACTTCACTAGTGTTT+GGG 0.319934 1.4:-57710865 None:intergenic
TTTGTTGCTGGAATTGCAAT+TGG 0.328084 1.4:+57710138 MS.gene39895:CDS
ACAAACAATTGGTTCTTTGA+TGG 0.342849 1.4:+57709842 MS.gene39895:CDS
TGACCAACTTCACTAGTGTT+TGG 0.354249 1.4:-57710866 None:intergenic
TCTTTGATGGCAACATATAT+TGG 0.360684 1.4:+57709855 MS.gene39895:CDS
CCTGCCACAGCTCCTCCACT+TGG 0.364123 1.4:-57710794 None:intergenic
ATGTACTAACTTGCACAAAA+AGG 0.373003 1.4:-57710896 None:intergenic
AATTTCCATGACACAATTGC+TGG 0.374440 1.4:-57710743 None:intergenic
GCACCAAGTAGTGGTTTGTC+TGG 0.377145 1.4:+57709055 MS.gene39895:CDS
GCAAGCACACAACACCAAAA+TGG 0.381298 1.4:+57709363 MS.gene39895:CDS
GGTGCATAATTTGAACCTTC+CGG 0.382873 1.4:-57709037 None:intergenic
TTCATTGATCCACCTGATAC+AGG 0.386394 1.4:-57710701 None:intergenic
ACAATAGCCTTTGCAACAAT+TGG 0.390249 1.4:+57709489 MS.gene39895:CDS
TATATTGGTAGCCTTGTCTA+TGG 0.405955 1.4:+57709870 MS.gene39895:CDS
CTGCCACAGCTCCTCCACTT+GGG 0.408595 1.4:-57710793 None:intergenic
AGGGTGTAACTCTGCCTTCT+GGG 0.416790 1.4:+57709929 MS.gene39895:CDS
TACATAATAGCACAAACAAT+TGG 0.418691 1.4:+57709831 MS.gene39895:CDS
CAACAATTGGTCACTTTCCA+TGG 0.426818 1.4:+57709502 MS.gene39895:CDS
GCAAAGGCTATTGTGATAGC+TGG 0.429353 1.4:-57709480 None:intergenic
GCTGGACCAAGTGATCTTGC+TGG 0.429509 1.4:-57710725 None:intergenic
CTAGGTAATGGCAGAGGTGG+TGG 0.457154 1.4:+57709302 MS.gene39895:intron
CCAAGTGGAGGAGCTGTGGC+AGG 0.470037 1.4:+57710794 MS.gene39895:CDS
CTGTAGCTGCATACTCCAAA+AGG 0.475436 1.4:-57709395 None:intergenic
TTGTTGCTGGAATTGCAATT+GGG 0.478564 1.4:+57710139 MS.gene39895:CDS
TTACCATTCGAGCGAAATTG+AGG 0.478978 1.4:-57709174 None:intergenic
TTGGAGTATGCAGCTACAGC+AGG 0.482843 1.4:+57709399 MS.gene39895:CDS
CACCAAGTAGTGGTTTGTCT+GGG 0.484402 1.4:+57709056 MS.gene39895:CDS
TCCCCAGACAAACCACTACT+TGG 0.487928 1.4:-57709058 None:intergenic
TGCCACAGCTCCTCCACTTG+GGG 0.492390 1.4:-57710792 None:intergenic
CTAGTGTTTGGGGAGCTACA+AGG 0.500939 1.4:-57710854 None:intergenic
TAACTCTGCCTTCTGGGTGG+AGG 0.509744 1.4:+57709935 MS.gene39895:CDS
CGACCTCAATTTCGCTCGAA+TGG 0.512673 1.4:+57709171 MS.gene39895:CDS
TCTGGGGATGATAAAGAGCT+TGG 0.521216 1.4:+57709073 MS.gene39895:CDS
TCCCCAAACACTAGTGAAGT+TGG 0.525887 1.4:+57710863 MS.gene39895:CDS
GTTGTGATAATCTTCTCTAT+AGG 0.527057 1.4:+57709432 MS.gene39895:CDS
TATGTAACAACCTTGGACCA+TGG 0.527508 1.4:-57709519 None:intergenic
CAACACCAAAATGGTGCAGT+TGG 0.532462 1.4:+57709372 MS.gene39895:CDS
TGTTGCAGCCCTGTATCAGG+TGG 0.533926 1.4:+57710692 MS.gene39895:intron
TGGTCACTTTCCATGGTCCA+AGG 0.541664 1.4:+57709509 MS.gene39895:CDS
TCAAATTATGCACCAAGTAG+TGG 0.544288 1.4:+57709046 MS.gene39895:CDS
CAAAGGCTATTGTGATAGCT+GGG 0.546101 1.4:-57709479 None:intergenic
AGCCCCAAGTGGAGGAGCTG+TGG 0.566367 1.4:+57710790 MS.gene39895:CDS
TGCAATAACCTCCACCCAGA+AGG 0.568635 1.4:-57709943 None:intergenic
TAGGTAATGGCAGAGGTGGT+GGG 0.568752 1.4:+57709303 MS.gene39895:intron
AGAAGGCAGAGTTACACCCT+TGG 0.582526 1.4:-57709926 None:intergenic
ATGAATCCAGCAAGATCACT+TGG 0.586915 1.4:+57710719 MS.gene39895:CDS
TCATTGATCCACCTGATACA+GGG 0.593857 1.4:-57710700 None:intergenic
AAAGTGACCAATTGTTGCAA+AGG 0.597190 1.4:-57709496 None:intergenic
ATGATGACTCAACCACTCCA+AGG 0.603566 1.4:+57709909 MS.gene39895:CDS
TTGGTCCAGCAATTGTGTCA+TGG 0.604068 1.4:+57710738 MS.gene39895:CDS
ACCAACTTCACTAGTGTTTG+GGG 0.606040 1.4:-57710864 None:intergenic
TGTTTGGGGAGCTACAAGGT+TGG 0.607612 1.4:-57710850 None:intergenic
GTGTAACTCTGCCTTCTGGG+TGG 0.612167 1.4:+57709932 MS.gene39895:CDS
GGCTTGCTGTACTAATTACA+GGG 0.627222 1.4:+57710160 MS.gene39895:CDS
AAGGTTGGTCTTTAAGACGT+AGG 0.629203 1.4:-57710835 None:intergenic
TGATGACTCAACCACTCCAA+GGG 0.629504 1.4:+57709910 MS.gene39895:CDS
TATCTAGGTAATGGCAGAGG+TGG 0.629546 1.4:+57709299 MS.gene39895:intron
ATGTATCTAGGTAATGGCAG+AGG 0.632592 1.4:+57709296 MS.gene39895:intron
TGTCTATGGCATAAAATCAG+AGG 0.635801 1.4:+57709884 MS.gene39895:CDS
GCAGAGTTACACCCTTGGAG+TGG 0.641435 1.4:-57709921 None:intergenic
AAACAATCATCATCTCCGGA+AGG 0.645392 1.4:+57709022 MS.gene39895:CDS
TACATCTTAGCCCCAAGTGG+AGG 0.646124 1.4:+57710782 MS.gene39895:CDS
ATATACATCTTAGCCCCAAG+TGG 0.684115 1.4:+57710779 MS.gene39895:CDS
ACCAAGTAGTGGTTTGTCTG+GGG 0.700105 1.4:+57709057 MS.gene39895:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTTATACTAAATGTT+AGG + chr1.4:57710325-57710344 MS.gene39895:intron 10.0%
!! AAAAATTAAAAATATTGTGC+AGG + chr1.4:57710098-57710117 MS.gene39895:intron 15.0%
!! AATTAGACAAAAATGTTTAT+GGG - chr1.4:57710575-57710594 None:intergenic 15.0%
!! TTTGTGATAAAAATGTTATA+TGG + chr1.4:57710601-57710620 MS.gene39895:intron 15.0%
!!! ATTTTTAGCTAGTAAAAAAA+TGG - chr1.4:57710084-57710103 None:intergenic 15.0%
!!! TTTGTATAATTTTTTTTCTC+CGG + chr1.4:57709620-57709639 MS.gene39895:intron 15.0%
!! ACTATTGACAAAATAATCAA+GGG - chr1.4:57709242-57709261 None:intergenic 20.0%
!! CAATTAGACAAAAATGTTTA+TGG - chr1.4:57710576-57710595 None:intergenic 20.0%
!! CTGTTTAAGAAACAATAAAA+GGG - chr1.4:57709802-57709821 None:intergenic 20.0%
!! CTTAACTTTCTCTTATAAAT+AGG + chr1.4:57709726-57709745 MS.gene39895:intron 20.0%
!! GTATAACACTTAAATTTGAT+TGG + chr1.4:57710257-57710276 MS.gene39895:intron 20.0%
!! TACATAAATAACTACTTAGT+CGG + chr1.4:57709536-57709555 MS.gene39895:intron 20.0%
!! TCTATTTATAAGAGAAAGTT+TGG + chr1.4:57709644-57709663 MS.gene39895:intron 20.0%
!!! AACATTCATCATTATGTTTT+TGG + chr1.4:57709965-57709984 MS.gene39895:CDS 20.0%
! AATTAAAAATATTGTGCAGG+TGG + chr1.4:57710101-57710120 MS.gene39895:intron 25.0%
! AGTTATTTATGTAACAACCT+TGG - chr1.4:57709529-57709548 None:intergenic 25.0%
! ATTAAAAATATTGTGCAGGT+GGG + chr1.4:57710102-57710121 MS.gene39895:intron 25.0%
! CAATCAGTAAGTACTAATTA+AGG + chr1.4:57710011-57710030 MS.gene39895:intron 25.0%
! CATGGAAATTTAAGAACATT+TGG + chr1.4:57710756-57710775 MS.gene39895:CDS 25.0%
! CCTGTTTAAGAAACAATAAA+AGG - chr1.4:57709803-57709822 None:intergenic 25.0%
! CTTTCTCTTATAAATAGAAC+CGG - chr1.4:57709642-57709661 None:intergenic 25.0%
! GACTATTGACAAAATAATCA+AGG - chr1.4:57709243-57709262 None:intergenic 25.0%
! GGAATCATTGCTAATTATTA+AGG - chr1.4:57710063-57710082 None:intergenic 25.0%
! TACATAATAGCACAAACAAT+TGG + chr1.4:57709831-57709850 MS.gene39895:CDS 25.0%
!! ATATTGATGTTTTGTGTATG+TGG + chr1.4:57709333-57709352 MS.gene39895:CDS 25.0%
!! GTTTGTGCTATTATGTAAAT+TGG - chr1.4:57709828-57709847 None:intergenic 25.0%
!!! AGTTATTTTGCTATGTATCT+AGG + chr1.4:57709284-57709303 MS.gene39895:intron 25.0%
!!! CCTTTTATTGTTTCTTAAAC+AGG + chr1.4:57709800-57709819 MS.gene39895:intron 25.0%
ACTTGACTATATAACTGGTT+GGG - chr1.4:57710517-57710536 None:intergenic 30.0%
AGTGATCAAATATATGTGAG+TGG - chr1.4:57710189-57710208 None:intergenic 30.0%
CACTTTAACCACTATTCTTA+AGG + chr1.4:57710362-57710381 MS.gene39895:intron 30.0%
CTAATACACCTTAAGAATAG+TGG - chr1.4:57710373-57710392 None:intergenic 30.0%
CTTTCTCTTATAAATAGGAC+CGG + chr1.4:57709731-57709750 MS.gene39895:intron 30.0%
GTGATCAAATATATGTGAGT+GGG - chr1.4:57710188-57710207 None:intergenic 30.0%
GTTGTGATAATCTTCTCTAT+AGG + chr1.4:57709432-57709451 MS.gene39895:CDS 30.0%
TATATAACTGGTTGGGTTAT+CGG - chr1.4:57710510-57710529 None:intergenic 30.0%
TCAACAAAACTTGATGTATC+TGG + chr1.4:57709667-57709686 MS.gene39895:intron 30.0%
TGAAAAACAATCATCATCTC+CGG + chr1.4:57709018-57709037 MS.gene39895:CDS 30.0%
TTGATTTCATTAGTACACTC+CGG - chr1.4:57709753-57709772 None:intergenic 30.0%
! AAACATAAAACGAAGAGTGT+TGG - chr1.4:57710445-57710464 None:intergenic 30.0%
! ACAAACAATTGGTTCTTTGA+TGG + chr1.4:57709842-57709861 MS.gene39895:CDS 30.0%
! ATCAATCCATCCATATCTTT+TGG + chr1.4:57709111-57709130 MS.gene39895:CDS 30.0%
! TCTTTGATGGCAACATATAT+TGG + chr1.4:57709855-57709874 MS.gene39895:CDS 30.0%
! TTTGCTATGTATCTAGGTAA+TGG + chr1.4:57709290-57709309 MS.gene39895:intron 30.0%
! TTTTAGCCAAAAGATATGGA+TGG - chr1.4:57709120-57709139 None:intergenic 30.0%
!!! GTGTTTTTAGCCAAAAGATA+TGG - chr1.4:57709124-57709143 None:intergenic 30.0%
AAAGTGACCAATTGTTGCAA+AGG - chr1.4:57709499-57709518 None:intergenic 35.0%
AATTTCCATGACACAATTGC+TGG - chr1.4:57710746-57710765 None:intergenic 35.0%
ACAATAGCCTTTGCAACAAT+TGG + chr1.4:57709489-57709508 MS.gene39895:CDS 35.0%
ACTGAAGTTGTGAAACATGA+CGG - chr1.4:57710484-57710503 None:intergenic 35.0%
CACTTGACTATATAACTGGT+TGG - chr1.4:57710518-57710537 None:intergenic 35.0%
TATGGGTATAGCATTCTAAG+TGG - chr1.4:57710558-57710577 None:intergenic 35.0%
TCAAATTATGCACCAAGTAG+TGG + chr1.4:57709046-57709065 MS.gene39895:CDS 35.0%
TGTCTATGGCATAAAATCAG+AGG + chr1.4:57709884-57709903 MS.gene39895:CDS 35.0%
TTGTTGCTGGAATTGCAATT+GGG + chr1.4:57710139-57710158 MS.gene39895:CDS 35.0%
TTTGTTGCTGGAATTGCAAT+TGG + chr1.4:57710138-57710157 MS.gene39895:CDS 35.0%
! CTAACTTGCACAAAAAGGTA+CGG - chr1.4:57710894-57710913 None:intergenic 35.0%
! CTGATTTTATGCCATAGACA+AGG - chr1.4:57709884-57709903 None:intergenic 35.0%
! TAACTTGCACAAAAAGGTAC+GGG - chr1.4:57710893-57710912 None:intergenic 35.0%
!! GAAATTGAGGTCGATTTTGT+TGG - chr1.4:57709164-57709183 None:intergenic 35.0%
!! TATATTGGTAGCCTTGTCTA+TGG + chr1.4:57709870-57709889 MS.gene39895:CDS 35.0%
!!! CATTTATCTGGTTTTGTTGC+TGG + chr1.4:57710126-57710145 MS.gene39895:CDS 35.0%
AAACAATCATCATCTCCGGA+AGG + chr1.4:57709022-57709041 MS.gene39895:CDS 40.0%
ACCAACTTCACTAGTGTTTG+GGG - chr1.4:57710867-57710886 None:intergenic 40.0%
ATATACATCTTAGCCCCAAG+TGG + chr1.4:57710779-57710798 MS.gene39895:CDS 40.0%
ATGAATCCAGCAAGATCACT+TGG + chr1.4:57710719-57710738 MS.gene39895:CDS 40.0%
CAAAGGCTATTGTGATAGCT+GGG - chr1.4:57709482-57709501 None:intergenic 40.0%
GACCAACTTCACTAGTGTTT+GGG - chr1.4:57710868-57710887 None:intergenic 40.0%
GGAATATGACATCACAACGA+TGG - chr1.4:57710424-57710443 None:intergenic 40.0%
GGTATAGCATTCTAAGTGGT+CGG - chr1.4:57710554-57710573 None:intergenic 40.0%
GGTGCATAATTTGAACCTTC+CGG - chr1.4:57709040-57709059 None:intergenic 40.0%
TATGTAACAACCTTGGACCA+TGG - chr1.4:57709522-57709541 None:intergenic 40.0%
TCATTGATCCACCTGATACA+GGG - chr1.4:57710703-57710722 None:intergenic 40.0%
TGACCAACTTCACTAGTGTT+TGG - chr1.4:57710869-57710888 None:intergenic 40.0%
TTACCATTCGAGCGAAATTG+AGG - chr1.4:57709177-57709196 None:intergenic 40.0%
TTCATTGATCCACCTGATAC+AGG - chr1.4:57710704-57710723 None:intergenic 40.0%
! ATGTATCTAGGTAATGGCAG+AGG + chr1.4:57709296-57709315 MS.gene39895:intron 40.0%
! CAACAATTGGTCACTTTCCA+TGG + chr1.4:57709502-57709521 MS.gene39895:CDS 40.0%
!! AAGGTTGGTCTTTAAGACGT+AGG - chr1.4:57710838-57710857 None:intergenic 40.0%
!! GGCTTGCTGTACTAATTACA+GGG + chr1.4:57710160-57710179 MS.gene39895:CDS 40.0%
ATGATGACTCAACCACTCCA+AGG + chr1.4:57709909-57709928 MS.gene39895:CDS 45.0%
CAACACCAAAATGGTGCAGT+TGG + chr1.4:57709372-57709391 MS.gene39895:CDS 45.0%
CTGTAGCTGCATACTCCAAA+AGG - chr1.4:57709398-57709417 None:intergenic 45.0%
GCAAAGGCTATTGTGATAGC+TGG - chr1.4:57709483-57709502 None:intergenic 45.0%
GCAAGCACACAACACCAAAA+TGG + chr1.4:57709363-57709382 MS.gene39895:CDS 45.0%
TCCCCAAACACTAGTGAAGT+TGG + chr1.4:57710863-57710882 MS.gene39895:CDS 45.0%
TCTGGGGATGATAAAGAGCT+TGG + chr1.4:57709073-57709092 MS.gene39895:CDS 45.0%
TGATGACTCAACCACTCCAA+GGG + chr1.4:57709910-57709929 MS.gene39895:CDS 45.0%
TTGGTCCAGCAATTGTGTCA+TGG + chr1.4:57710738-57710757 MS.gene39895:CDS 45.0%
! AAAGGCCAACTGCACCATTT+TGG - chr1.4:57709380-57709399 None:intergenic 45.0%
! ACCAAGTAGTGGTTTGTCTG+GGG + chr1.4:57709057-57709076 MS.gene39895:CDS 45.0%
! CACCAAGTAGTGGTTTGTCT+GGG + chr1.4:57709056-57709075 MS.gene39895:CDS 45.0%
! GGGGCACTTGACTATATAAC+TGG - chr1.4:57710522-57710541 None:intergenic 45.0%
! TATCTAGGTAATGGCAGAGG+TGG + chr1.4:57709299-57709318 MS.gene39895:intron 45.0%
! TTTTGTTGCAGCCCTGTATC+AGG + chr1.4:57710689-57710708 MS.gene39895:intron 45.0%
!! GGGCTTGCTGTACTAATTAC+AGG + chr1.4:57710159-57710178 MS.gene39895:CDS 45.0%
!!! AAATGGTGCAGTTGGCCTTT+TGG + chr1.4:57709380-57709399 MS.gene39895:CDS 45.0%
AGAAGGCAGAGTTACACCCT+TGG - chr1.4:57709929-57709948 None:intergenic 50.0%
AGGGTGTAACTCTGCCTTCT+GGG + chr1.4:57709929-57709948 MS.gene39895:CDS 50.0%
CGACCTCAATTTCGCTCGAA+TGG + chr1.4:57709171-57709190 MS.gene39895:CDS 50.0%
CTAGTGTTTGGGGAGCTACA+AGG - chr1.4:57710857-57710876 None:intergenic 50.0%
GTGCAGGTGGGACATTTATC+TGG + chr1.4:57710114-57710133 MS.gene39895:intron 50.0%
TACATCTTAGCCCCAAGTGG+AGG + chr1.4:57710782-57710801 MS.gene39895:CDS 50.0%
TCCCCAGACAAACCACTACT+TGG - chr1.4:57709061-57709080 None:intergenic 50.0%
TGCAATAACCTCCACCCAGA+AGG - chr1.4:57709946-57709965 None:intergenic 50.0%
TGTTTGGGGAGCTACAAGGT+TGG - chr1.4:57710853-57710872 None:intergenic 50.0%
TTGGAGTATGCAGCTACAGC+AGG + chr1.4:57709399-57709418 MS.gene39895:CDS 50.0%
! AAGGGTGTAACTCTGCCTTC+TGG + chr1.4:57709928-57709947 MS.gene39895:CDS 50.0%
! GCACCAAGTAGTGGTTTGTC+TGG + chr1.4:57709055-57709074 MS.gene39895:CDS 50.0%
! TAGGTAATGGCAGAGGTGGT+GGG + chr1.4:57709303-57709322 MS.gene39895:intron 50.0%
! TGGTCACTTTCCATGGTCCA+AGG + chr1.4:57709509-57709528 MS.gene39895:CDS 50.0%
ATTCTAAGTGGTCGGCCTCG+AGG - chr1.4:57710546-57710565 None:intergenic 55.0%
GCAGAGTTACACCCTTGGAG+TGG - chr1.4:57709924-57709943 None:intergenic 55.0%
GCTGGACCAAGTGATCTTGC+TGG - chr1.4:57710728-57710747 None:intergenic 55.0%
GTGTAACTCTGCCTTCTGGG+TGG + chr1.4:57709932-57709951 MS.gene39895:CDS 55.0%
TAACTCTGCCTTCTGGGTGG+AGG + chr1.4:57709935-57709954 MS.gene39895:CDS 55.0%
TGTTGCAGCCCTGTATCAGG+TGG + chr1.4:57710692-57710711 MS.gene39895:intron 55.0%
! CTAGGTAATGGCAGAGGTGG+TGG + chr1.4:57709302-57709321 MS.gene39895:intron 55.0%
CTGCCACAGCTCCTCCACTT+GGG - chr1.4:57710796-57710815 None:intergenic 60.0%
TAAGTGGTCGGCCTCGAGGA+GGG - chr1.4:57710542-57710561 None:intergenic 60.0%
TAGTCAAGTGCCCCTCCTCG+AGG + chr1.4:57710528-57710547 MS.gene39895:intron 60.0%
TGCCACAGCTCCTCCACTTG+GGG - chr1.4:57710795-57710814 None:intergenic 60.0%
AAGTGGTCGGCCTCGAGGAG+GGG - chr1.4:57710541-57710560 None:intergenic 65.0%
AGCCCCAAGTGGAGGAGCTG+TGG + chr1.4:57710790-57710809 MS.gene39895:CDS 65.0%
CCAAGTGGAGGAGCTGTGGC+AGG + chr1.4:57710794-57710813 MS.gene39895:CDS 65.0%
CCTGCCACAGCTCCTCCACT+TGG - chr1.4:57710797-57710816 None:intergenic 65.0%
CTAAGTGGTCGGCCTCGAGG+AGG - chr1.4:57710543-57710562 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 57709004 57710913 57709004 ID=MS.gene39895
chr1.4 mRNA 57709004 57710913 57709004 ID=MS.gene39895.t1;Parent=MS.gene39895
chr1.4 exon 57709004 57709192 57709004 ID=MS.gene39895.t1.exon1;Parent=MS.gene39895.t1
chr1.4 CDS 57709004 57709192 57709004 ID=cds.MS.gene39895.t1;Parent=MS.gene39895.t1
chr1.4 exon 57709306 57709530 57709306 ID=MS.gene39895.t1.exon2;Parent=MS.gene39895.t1
chr1.4 CDS 57709306 57709530 57709306 ID=cds.MS.gene39895.t1;Parent=MS.gene39895.t1
chr1.4 exon 57709822 57710016 57709822 ID=MS.gene39895.t1.exon3;Parent=MS.gene39895.t1
chr1.4 CDS 57709822 57710016 57709822 ID=cds.MS.gene39895.t1;Parent=MS.gene39895.t1
chr1.4 exon 57710120 57710181 57710120 ID=MS.gene39895.t1.exon4;Parent=MS.gene39895.t1
chr1.4 CDS 57710120 57710181 57710120 ID=cds.MS.gene39895.t1;Parent=MS.gene39895.t1
chr1.4 exon 57710700 57710913 57710700 ID=MS.gene39895.t1.exon5;Parent=MS.gene39895.t1
chr1.4 CDS 57710700 57710913 57710700 ID=cds.MS.gene39895.t1;Parent=MS.gene39895.t1
Gene Sequence

>MS.gene39895

ATGAGAGACATATTTGAAAAACAATCATCATCTCCGGAAGGTTCAAATTATGCACCAAGTAGTGGTTTGTCTGGGGATGATAAAGAGCTTGGCTATAGAGCTGCAACATCAATCCATCCATATCTTTTGGCTAAAAACACTTATCTTCATTTCTTAGCCAACAAAATCGACCTCAATTTCGCTCGAATGGTAATAGTATATTCAACATTTTTATGTGTATACAGAATGCAATTGTCCCTTGATTATTTTGTCAATAGTCTTTTGATAAATGCAAAATTGAAGTTATTTTGCTATGTATCTAGGTAATGGCAGAGGTGGTGGGTACTTTTATATTGATGTTTTGTGTATGTGGAATCATTGCAAGCACACAACACCAAAATGGTGCAGTTGGCCTTTTGGAGTATGCAGCTACAGCAGGATTAACAGTCGTTGTGATAATCTTCTCTATAGGCTCAATTTCTTGTGCACATGTTAACCCAGCTATCACAATAGCCTTTGCAACAATTGGTCACTTTCCATGGTCCAAGGTTGTTACATAAATAACTACTTAGTCGGTTAAACTTAAAATTTAGATAGTATATCTGATTGATTTATAGAGTTTAATTTTTATGTATTTTTTGTATAATTTTTTTTCTCCGGTTCTATTTATAAGAGAAAGTTTGGTCAACAAAACTTGATGTATCTGGTTAAAAATTCAGTCAAGATACATCAATTTTTGTTGACTTAACTTTCTCTTATAAATAGGACCGGAGTGTACTAATGAAATCAAATTTATAACTATATAATATATACATACCCTTTTATTGTTTCTTAAACAGGTTCCAATTTACATAATAGCACAAACAATTGGTTCTTTGATGGCAACATATATTGGTAGCCTTGTCTATGGCATAAAATCAGAGGTTATGATGACTCAACCACTCCAAGGGTGTAACTCTGCCTTCTGGGTGGAGGTTATTGCAACATTCATCATTATGTTTTTGGTTACAGCTTTGACATCTGAATCTCAATCAGTAAGTACTAATTAAGGAAAAAAATTACATAGTTATATATGTTCCTTAATAATTAGCAATGATTCCATTTTTTTACTAGCTAAAAATTAAAAATATTGTGCAGGTGGGACATTTATCTGGTTTTGTTGCTGGAATTGCAATTGGGCTTGCTGTACTAATTACAGGGTACCCACTCACATATATTTGATCACTTTGATATTTATCGAGATATTACTTTGAGAAATAGTATAATAATCTATAGTATAACACTTAAATTTGATTGGATATCTGTTTAAAATTCTTTATATGATTAGTAGTACGTAGTTATTAAATTTTTATACTAAATGTTAGGTCAGCTATGATTATCACTTTAACCACTATTCTTAAGGTGTATTAGTCTATTTGATCATGCTTTGTGTTGTGCACCATCGTTGTGATGTCATATTCCAACACTCTTCGTTTTATGTTTATGAAATTCACTCTGACCGTCATGTTTCACAACTTCAGTGAACCGATAACCCAACCAGTTATATAGTCAAGTGCCCCTCCTCGAGGCCGACCACTTAGAATGCTATACCCATAAACATTTTTGTCTAATTGTGTATTTTGTGATAAAAATGTTATATGGATTAATTCTATATCTTACTCTTTAACATGATACTTTAATCTAATAACAAAGTAATTTGATTTAATTTTTGTTGCAGCCCTGTATCAGGTGGATCAATGAATCCAGCAAGATCACTTGGTCCAGCAATTGTGTCATGGAAATTTAAGAACATTTGGATATACATCTTAGCCCCAAGTGGAGGAGCTGTGGCAGGAGCTCTAATGTTTCGTTTCCTACGTCTTAAAGACCAACCTTGTAGCTCCCCAAACACTAGTGAAGTTGGTCATCCCGTACCTTTTTGTGCAAGTTAG
Protein sequence

>MS.gene39895.t1

MRDIFEKQSSSPEGSNYAPSSGLSGDDKELGYRAATSIHPYLLAKNTYLHFLANKIDLNFARMVMAEVVGTFILMFCVCGIIASTQHQNGAVGLLEYAATAGLTVVVIIFSIGSISCAHVNPAITIAFATIGHFPWSKVPIYIIAQTIGSLMATYIGSLVYGIKSEVMMTQPLQGCNSAFWVEVIATFIIMFLVTALTSESQSVGHLSGFVAGIAIGLAVLITGPVSGGSMNPARSLGPAIVSWKFKNIWIYILAPSGGAVAGALMFRFLRLKDQPCSSPNTSEVGHPVPFCAS