AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004322

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004322.t1 MTR_2g017590 94.834 271 13 1 1 271 1 270 0.0 516
MS.gene004322.t1 MTR_2g017620 89.259 270 27 1 1 268 1 270 5.66e-176 486
MS.gene004322.t1 MTR_2g017620 90.234 256 25 0 13 268 1 256 3.63e-170 470
MS.gene004322.t1 MTR_2g017570 79.775 267 54 0 5 271 7 273 1.76e-160 446
MS.gene004322.t1 MTR_2g017610 79.775 267 54 0 5 271 7 273 1.80e-160 446
MS.gene004322.t1 MTR_8g087720 75.096 261 65 0 5 265 7 267 8.53e-146 409
MS.gene004322.t1 MTR_2g017660 65.942 276 85 2 1 267 1 276 2.74e-133 378
MS.gene004322.t1 MTR_8g087710 66.794 262 87 0 10 271 13 274 5.30e-131 372
MS.gene004322.t1 MTR_2g017620 91.005 189 17 0 80 268 1 189 2.18e-123 349
MS.gene004322.t1 MTR_8g087720 74.359 195 50 0 5 199 7 201 8.74e-103 298
MS.gene004322.t1 MTR_4g084880 56.838 234 99 1 32 263 31 264 6.18e-99 291
MS.gene004322.t1 MTR_8g087720 71.910 178 50 0 5 182 7 184 1.39e-89 264
MS.gene004322.t1 MTR_5g079570 52.209 249 110 3 6 251 12 254 7.67e-87 260
MS.gene004322.t1 MTR_3g008920 52.301 239 111 2 16 254 8 243 1.00e-82 249
MS.gene004322.t1 MTR_4g006730 50.000 246 122 1 15 260 54 298 8.96e-75 230
MS.gene004322.t1 MTR_2g104940 50.000 252 117 3 14 260 56 303 1.31e-74 230
MS.gene004322.t1 MTR_7g010650 48.458 227 115 2 36 262 37 261 6.27e-72 222
MS.gene004322.t1 MTR_1g097840 47.471 257 120 5 13 257 47 300 1.14e-68 214
MS.gene004322.t1 MTR_5g063930 45.113 266 131 6 1 258 1 259 2.02e-68 213
MS.gene004322.t1 MTR_4g006630 51.887 212 101 1 49 260 1 211 9.95e-68 209
MS.gene004322.t1 MTR_4g006730 51.887 212 101 1 49 260 1 211 1.59e-67 209
MS.gene004322.t1 MTR_1g070385 43.662 213 119 1 38 250 64 275 1.36e-51 180
MS.gene004322.t1 MTR_4g006730 44.444 216 107 2 14 229 53 255 1.35e-49 164
MS.gene004322.t1 MTR_1g097840 52.632 171 74 2 32 199 70 236 2.46e-45 154
MS.gene004322.t1 MTR_7g010650 44.444 144 79 1 36 179 37 179 1.35e-38 134
MS.gene004322.t1 MTR_8g013680 33.193 238 148 6 28 254 9 246 1.75e-28 109
MS.gene004322.t1 MTR_2g101370 34.274 248 145 8 19 254 6 247 1.39e-27 107
MS.gene004322.t1 MTR_1g077540 32.510 243 153 6 19 251 2 243 2.29e-27 106
MS.gene004322.t1 MTR_6g069040 32.129 249 146 6 19 251 2 243 2.44e-27 106
MS.gene004322.t1 MTR_1g006490 38.389 211 120 7 35 238 18 225 6.02e-27 105
MS.gene004322.t1 MTR_4g006650 45.517 145 63 3 37 178 5 136 5.83e-26 104
MS.gene004322.t1 MTR_5g012810 35.849 212 127 5 35 238 18 228 1.04e-23 96.7
MS.gene004322.t1 MTR_1g006590 33.906 233 145 6 35 261 18 247 5.32e-23 94.7
MS.gene004322.t1 MTR_4g063090 34.081 223 138 5 41 255 26 247 1.84e-22 93.6
MS.gene004322.t1 MTR_7g103030 34.123 211 131 4 31 234 16 225 2.88e-22 92.8
MS.gene004322.t1 MTR_1g115405 31.624 234 152 4 35 261 20 252 2.53e-20 87.8
MS.gene004322.t1 MTR_5g010150 31.944 216 125 6 39 235 55 267 4.01e-17 79.7
MS.gene004322.t1 MTR_7g023350 30.303 231 154 3 35 258 20 250 5.48e-17 78.6
MS.gene004322.t1 MTR_7g101190 29.231 260 162 7 25 262 28 287 7.49e-17 79.0
MS.gene004322.t1 MTR_5g082070 31.481 216 126 6 39 235 58 270 8.31e-17 78.6
MS.gene004322.t1 MTR_2g094270 32.275 189 115 4 59 235 74 261 1.03e-16 78.6
MS.gene004322.t1 MTR_3g118010 28.194 227 148 4 34 245 35 261 3.07e-16 77.0
MS.gene004322.t1 MTR_3g070210 31.776 214 128 7 39 235 58 270 4.51e-16 76.6
MS.gene004322.t1 MTR_8g098375 31.481 216 126 6 39 235 55 267 9.33e-16 75.9
MS.gene004322.t1 MTR_4g415300 30.660 212 132 6 39 235 59 270 1.23e-15 75.5
MS.gene004322.t1 MTR_2g060360 31.336 217 137 5 35 242 18 231 7.47e-15 72.8
MS.gene004322.t1 MTR_2g094270 31.214 173 109 3 59 222 74 245 1.08e-13 69.7
MS.gene004322.t1 MTR_3g070210 31.156 199 122 6 39 223 58 255 1.73e-13 69.3
MS.gene004322.t1 MTR_1g095070 29.121 182 111 4 79 245 25 203 5.01e-13 67.0
MS.gene004322.t1 MTR_4g059390 28.319 226 135 7 34 235 37 259 1.07e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004322.t1 AT4G18910 67.871 249 73 1 22 263 38 286 2.53e-124 356
MS.gene004322.t1 AT4G19030 63.813 257 86 1 14 263 33 289 1.63e-120 346
MS.gene004322.t1 AT5G37820 59.438 249 98 1 20 268 30 275 3.34e-102 299
MS.gene004322.t1 AT5G37820 59.438 249 98 1 20 268 66 311 1.06e-101 299
MS.gene004322.t1 AT5G37810 58.091 241 98 1 23 263 33 270 7.34e-98 288
MS.gene004322.t1 AT1G31885 50.000 270 120 3 1 257 4 271 1.02e-92 277
MS.gene004322.t1 AT4G18910 68.649 185 51 1 22 199 38 222 5.78e-87 258
MS.gene004322.t1 AT4G19030 66.486 185 55 1 22 199 41 225 2.19e-85 254
MS.gene004322.t1 AT2G34390 49.301 286 117 6 1 263 1 281 5.46e-85 256
MS.gene004322.t1 AT1G31885 54.839 217 91 1 48 257 1 217 7.89e-85 254
MS.gene004322.t1 AT2G34390 53.680 231 99 2 37 260 7 236 8.78e-83 249
MS.gene004322.t1 AT1G80760 51.020 245 115 2 8 250 52 293 3.89e-74 229
MS.gene004322.t1 AT4G19030 61.272 173 60 1 14 179 33 205 1.25e-73 224
MS.gene004322.t1 AT4G10380 49.200 250 116 3 18 257 56 304 1.02e-70 220
MS.gene004322.t1 AT2G34390 50.901 222 81 7 1 199 1 217 4.60e-64 200
MS.gene004322.t1 AT2G34390 55.414 157 62 2 32 181 44 199 1.52e-55 179
MS.gene004322.t1 AT3G06100 43.119 218 122 2 35 251 45 261 7.27e-52 171
MS.gene004322.t1 AT3G06100 43.458 214 119 2 39 251 1 213 2.26e-51 168
MS.gene004322.t1 AT3G16240 36.866 217 128 4 27 234 8 224 5.65e-32 118
MS.gene004322.t1 AT2G29870 55.789 95 42 0 151 245 20 114 7.85e-31 112
MS.gene004322.t1 AT1G31885 46.610 118 55 2 1 112 4 119 8.96e-31 112
MS.gene004322.t1 AT5G47450 37.004 227 133 5 22 238 3 229 3.64e-30 114
MS.gene004322.t1 AT3G06100 39.437 142 84 2 112 252 41 181 8.99e-27 103
MS.gene004322.t1 AT4G17340 34.802 227 138 5 22 238 3 229 1.34e-26 104
MS.gene004322.t1 AT1G17810 30.916 262 142 6 22 258 13 260 5.16e-26 103
MS.gene004322.t1 AT1G73190 31.349 252 138 7 22 251 13 251 1.48e-24 99.4
MS.gene004322.t1 AT3G26520 32.365 241 144 6 31 258 17 251 8.02e-23 94.7
MS.gene004322.t1 AT2G25810 36.493 211 124 7 35 238 18 225 6.53e-22 92.0
MS.gene004322.t1 AT2G36830 31.441 229 147 4 13 234 1 226 8.95e-22 91.7
MS.gene004322.t1 AT4G01470 33.766 231 141 6 13 235 1 227 1.59e-20 88.2
MS.gene004322.t1 AT5G60660 28.458 253 153 7 33 261 36 284 1.01e-18 84.0
MS.gene004322.t1 AT4G35100 30.396 227 143 5 34 245 36 262 3.32e-18 82.4
MS.gene004322.t1 AT4G35100 30.396 227 143 5 34 245 36 262 3.32e-18 82.4
MS.gene004322.t1 AT2G16850 29.130 230 142 5 34 245 34 260 4.41e-18 82.0
MS.gene004322.t1 AT2G39010 31.771 192 119 4 66 245 77 268 2.44e-17 80.1
MS.gene004322.t1 AT3G47440 30.045 223 132 6 35 242 22 235 6.34e-17 78.6
MS.gene004322.t1 AT1G01620 31.628 215 126 5 39 235 55 266 8.48e-17 78.6
MS.gene004322.t1 AT3G61430 31.163 215 127 5 39 235 55 266 1.11e-16 78.6
MS.gene004322.t1 AT3G61430 31.163 215 127 5 39 235 55 266 1.11e-16 78.6
MS.gene004322.t1 AT4G00430 31.628 215 126 5 39 235 56 267 1.47e-16 78.2
MS.gene004322.t1 AT1G01620 33.158 190 109 4 61 235 8 194 1.48e-16 76.6
MS.gene004322.t1 AT3G53420 30.890 191 114 4 70 245 82 269 3.30e-16 77.0
MS.gene004322.t1 AT3G53420 30.890 191 114 4 70 245 82 269 3.30e-16 77.0
MS.gene004322.t1 AT4G23400 31.163 215 127 5 39 235 56 267 6.27e-16 76.3
MS.gene004322.t1 AT2G37170 27.542 236 144 6 34 245 35 267 3.17e-15 74.3
MS.gene004322.t1 AT3G54820 27.468 233 148 5 34 245 36 268 3.18e-15 74.3
MS.gene004322.t1 AT2G45960 30.698 215 128 5 39 235 55 266 3.27e-15 74.3
MS.gene004322.t1 AT2G37170 27.542 236 144 6 34 245 78 310 5.33e-15 73.9
MS.gene004322.t1 AT2G37180 27.542 236 144 6 34 245 35 267 1.29e-14 72.4
MS.gene004322.t1 AT2G45960 30.964 197 118 4 39 220 55 248 3.40e-13 68.6
MS.gene004322.t1 AT2G45960 30.964 197 118 4 39 220 55 248 4.70e-13 68.2

Find 76 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTTATGTTCATCATTTC+TGG 0.019796 2.3:-68643826 MS.gene004322:CDS
TCCTGGAATTTCTATTGTTT+GGG 0.178041 2.3:-68644800 MS.gene004322:CDS
TTCCTGGAATTTCTATTGTT+TGG 0.214894 2.3:-68644801 MS.gene004322:CDS
CCTTGCTTCCTGCACCTTTG+AGG 0.279429 2.3:+68643086 None:intergenic
ACATTCTACTTGATATTTGC+TGG 0.321198 2.3:-68644871 MS.gene004322:CDS
TGTTTGGGGACTTGCTGTTA+TGG 0.331507 2.3:-68644785 MS.gene004322:CDS
GTCACCTATTCTAGGAGCTT+TGG 0.344731 2.3:-68643182 MS.gene004322:CDS
GATAAAGTAGTGACACTTCC+TGG 0.350687 2.3:-68644817 MS.gene004322:CDS
TCCATATTCCTCTATATTCA+TGG 0.360944 2.3:+68643215 None:intergenic
ATTGCTTTAGATTGGTGAAT+TGG 0.362837 2.3:-68643497 MS.gene004322:intron
GCTGGTCCTAAGCTTCTTGC+TGG 0.364030 2.3:+68643247 None:intergenic
GGCAAGGAAAATCAATTTGC+AGG 0.368386 2.3:-68643907 MS.gene004322:CDS
ATACTAAATGTGTTGTTTGC+GGG 0.374820 2.3:-68643448 MS.gene004322:CDS
TATGTTGTGTCACCTATTCT+AGG 0.387825 2.3:-68643190 MS.gene004322:CDS
TCACTACTTTATCATTGTTA+AGG 0.387999 2.3:+68644827 None:intergenic
ATGGTATTAGTATATTCTAT+TGG 0.391418 2.3:-68644766 MS.gene004322:CDS
CTGCTTCCATGGAAACCTTC+CGG 0.415154 2.3:+68644690 None:intergenic
TACTTGATATTTGCTGGATG+TGG 0.422703 2.3:-68644865 MS.gene004322:CDS
CTCCAGAAATGATGAACATA+AGG 0.423335 2.3:+68643824 None:intergenic
CTTTAGATTGGTGAATTGGC+TGG 0.424124 2.3:-68643493 MS.gene004322:intron
TATTCTATTGGCCATATCTC+TGG 0.439778 2.3:-68644754 MS.gene004322:CDS
CCCCAAACAATAGAAATTCC+AGG 0.443536 2.3:+68644799 None:intergenic
TGAGATGGGCACCAGAGATA+TGG 0.444610 2.3:+68644743 None:intergenic
CCAGCCAAAGCTCCTAGAAT+AGG 0.460240 2.3:+68643178 None:intergenic
TAGGAGCTTTGGCTGGTACA+TGG 0.462758 2.3:-68643171 MS.gene004322:CDS
GCTCTCAAACTTATATTCAA+TGG 0.464325 2.3:-68643928 MS.gene004322:CDS
ATTCATTGATGCTCCTGTGA+TGG 0.469127 2.3:+68643270 None:intergenic
TTTGAGGAATGAAGAACTCT+TGG 0.469564 2.3:+68643102 None:intergenic
TATACTAAATGTGTTGTTTG+CGG 0.470231 2.3:-68643449 MS.gene004322:CDS
TTCATTGATGCTCCTGTGAT+GGG 0.472526 2.3:+68643271 None:intergenic
TGGCGATTTACCTGCTTCCA+TGG 0.482498 2.3:+68644679 None:intergenic
ATGAATCCAGCAAGAAGCTT+AGG 0.484395 2.3:-68643253 MS.gene004322:CDS
TATATAATTGCTCAGGTCGT+TGG 0.487350 2.3:-68643970 MS.gene004322:CDS
TATTCATGGTGCACAATAGC+TGG 0.496895 2.3:+68643229 None:intergenic
TTTGCAGGAACACTTCCTGC+TGG 0.505103 2.3:-68643892 MS.gene004322:CDS
TCTTCATTCCTCAAAGGTGC+AGG 0.507670 2.3:-68643094 MS.gene004322:CDS
TGGTGACAGCGGGATTGAGA+TGG 0.513241 2.3:+68644728 None:intergenic
AATATAGTTGGTAGCAGAAG+TGG 0.514295 2.3:-68644899 MS.gene004322:intron
GAATTGGCTGGACTTGCAGT+TGG 0.518324 2.3:-68643481 MS.gene004322:CDS
ACCTGAGCAATTATATAAGC+TGG 0.518659 2.3:+68643976 None:intergenic
ACCAGCTTATATAATTGCTC+AGG 0.519565 2.3:-68643977 MS.gene004322:CDS
CCTGGAATTTCTATTGTTTG+GGG 0.522115 2.3:-68644799 MS.gene004322:CDS
GAAACCTTCCGGTGGTAGTG+TGG 0.524431 2.3:+68644701 None:intergenic
CCTATTCTAGGAGCTTTGGC+TGG 0.527244 2.3:-68643178 MS.gene004322:CDS
CTCTTGGTGAGCTCACGTAC+TGG 0.527633 2.3:+68643118 None:intergenic
ACCATGAATATAGAGGAATA+TGG 0.529709 2.3:-68643216 MS.gene004322:CDS
CGGAAGGTTTCCATGGAAGC+AGG 0.534310 2.3:-68644689 MS.gene004322:intron
CCTCAAAGGTGCAGGAAGCA+AGG 0.539036 2.3:-68643086 MS.gene004322:CDS
CTACCACCGGAAGGTTTCCA+TGG 0.544728 2.3:-68644696 MS.gene004322:CDS
CTCAAAGGTGCAGGAAGCAA+GGG 0.548913 2.3:-68643085 MS.gene004322:CDS
TACTAAATGTGTTGTTTGCG+GGG 0.554404 2.3:-68643447 MS.gene004322:intron
GTGTGGGCAATGGTGACAGC+GGG 0.556492 2.3:+68644718 None:intergenic
GGTGACAGCGGGATTGAGAT+GGG 0.557496 2.3:+68644729 None:intergenic
AACATGGGTGATATTAGCAA+TGG 0.558983 2.3:-68645110 MS.gene004322:CDS
ACCATTGCCCACACTACCAC+CGG 0.559073 2.3:-68644709 MS.gene004322:CDS
CTGGCTTATTAGTGATTCTG+AGG 0.564075 2.3:+68643137 None:intergenic
TGCTGGATGTGGTTCTGTGG+TGG 0.564205 2.3:-68644854 MS.gene004322:CDS
ATAGTTGGTAGCAGAAGTGG+TGG 0.567543 2.3:-68644896 MS.gene004322:intron
ATTTGCTGGATGTGGTTCTG+TGG 0.593346 2.3:-68644857 MS.gene004322:CDS
GCTTGAAGATCAGAACCAGC+AGG 0.594492 2.3:+68643877 None:intergenic
CAAACTTATATTCAATGGCA+AGG 0.602566 2.3:-68643923 MS.gene004322:CDS
AAGAGTTCTTCATTCCTCAA+AGG 0.603468 2.3:-68643100 MS.gene004322:CDS
AAACCTTCCGGTGGTAGTGT+GGG 0.610875 2.3:+68644702 None:intergenic
AGTGATTCTGAGGAAAGTGT+AGG 0.628271 2.3:+68643147 None:intergenic
TCCGGTGGTAGTGTGGGCAA+TGG 0.629620 2.3:+68644708 None:intergenic
ATATTTGACAGACCCATCAC+AGG 0.629986 2.3:-68643283 MS.gene004322:intron
GATTACCGCTCTATTATCAG+TGG 0.633724 2.3:+68643798 None:intergenic
GTTGGCTCAACACTTGCAAG+TGG 0.642506 2.3:-68643952 MS.gene004322:CDS
ATTGTGCACCATGAATATAG+AGG 0.654237 2.3:-68643223 MS.gene004322:CDS
TAGCAATGGAAATCTTGATG+TGG 0.656350 2.3:-68645096 MS.gene004322:CDS
TTGCCCACACTACCACCGGA+AGG 0.659217 2.3:-68644705 MS.gene004322:CDS
CTTCCATGGAAACCTTCCGG+TGG 0.678612 2.3:+68644693 None:intergenic
AGTGTGGGCAATGGTGACAG+CGG 0.685972 2.3:+68644717 None:intergenic
TAGTTGGTAGCAGAAGTGGT+GGG 0.737512 2.3:-68644895 MS.gene004322:CDS
AGTTGCCACTGATAATAGAG+CGG 0.742875 2.3:-68643803 MS.gene004322:intron
GTGGTTATGAATGTAAACGA+TGG 0.756470 2.3:-68645077 MS.gene004322:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATATAATGTATAATCT+TGG + chr2.3:68644813-68644832 None:intergenic 10.0%
!!! AAAAAATTAATTGTTGTTTA+TGG - chr2.3:68643206-68643225 MS.gene004322:CDS 10.0%
!!! TAAACAACAATTAATTTTTT+TGG + chr2.3:68643206-68643225 None:intergenic 10.0%
!!! TTTTTTAAAAAAAAAATGCT+TGG - chr2.3:68644407-68644426 MS.gene004322:intron 10.0%
!! TAGATGTATAAATATATTCT+AGG - chr2.3:68644574-68644593 MS.gene004322:intron 15.0%
!!! AGAAAAAGTCAATTTTATTT+CGG + chr2.3:68644527-68644546 None:intergenic 15.0%
!!! TATTATTTATTGCTTTAGAT+TGG - chr2.3:68644677-68644696 MS.gene004322:intron 15.0%
!! AAAAATTCAAAACTCTTTAG+AGG + chr2.3:68643656-68643675 None:intergenic 20.0%
!! ATGGTATTAGTATATTCTAT+TGG - chr2.3:68643416-68643435 MS.gene004322:intron 20.0%
!! GCACTTTATGATATAATATT+TGG + chr2.3:68644780-68644799 None:intergenic 20.0%
!! TATACAAAAACTATTAGACA+AGG + chr2.3:68644653-68644672 None:intergenic 20.0%
!! TCTTATATAGTTGAGAAAAT+AGG - chr2.3:68643715-68643734 MS.gene004322:intron 20.0%
!! TTGCCTTAATATGAATTATA+GGG + chr2.3:68643538-68643557 None:intergenic 20.0%
!! TTTGCCTTAATATGAATTAT+AGG + chr2.3:68643539-68643558 None:intergenic 20.0%
!!! AAAAATGTTGTTTTCGTTAT+AGG + chr2.3:68644144-68644163 None:intergenic 20.0%
!!! ATAACGAAAACAACATTTTT+AGG - chr2.3:68644144-68644163 MS.gene004322:intron 20.0%
!!! CTAAAGAGTTTTGAATTTTT+GGG - chr2.3:68643656-68643675 MS.gene004322:intron 20.0%
!!! TAAAGAGTTTTGAATTTTTG+GGG - chr2.3:68643657-68643676 MS.gene004322:intron 20.0%
!!! TCTAAAGAGTTTTGAATTTT+TGG - chr2.3:68643655-68643674 MS.gene004322:intron 20.0%
! ATCTCGTTAAGTTATTTGAT+CGG - chr2.3:68643574-68643593 MS.gene004322:intron 25.0%
! ATGCCCTATAATTCATATTA+AGG - chr2.3:68643532-68643551 MS.gene004322:intron 25.0%
! CTTTGATTGTTGAATATAGT+TGG - chr2.3:68643271-68643290 MS.gene004322:CDS 25.0%
! GATGTTCTGACTAAAAAAAA+TGG - chr2.3:68643240-68643259 MS.gene004322:CDS 25.0%
! TCACTACTTTATCATTGTTA+AGG + chr2.3:68643358-68643377 None:intergenic 25.0%
!! TATTTTCTTCTTCTTCACAT+AGG + chr2.3:68643924-68643943 None:intergenic 25.0%
!!! TATACTAAATGTGTTGTTTG+CGG - chr2.3:68644733-68644752 MS.gene004322:CDS 25.0%
!!! TGTACTTAATTTTTTCTTGC+AGG - chr2.3:68644181-68644200 MS.gene004322:intron 25.0%
ACATTCTACTTGATATTTGC+TGG - chr2.3:68643311-68643330 MS.gene004322:intron 30.0%
ACCATGAATATAGAGGAATA+TGG - chr2.3:68644966-68644985 MS.gene004322:intron 30.0%
AGAGATAGTGACTTAAATTG+TGG + chr2.3:68644035-68644054 None:intergenic 30.0%
ATAGTTGGAAGACCATTATT+TGG - chr2.3:68643941-68643960 MS.gene004322:CDS 30.0%
CAAACTTATATTCAATGGCA+AGG - chr2.3:68644259-68644278 MS.gene004322:intron 30.0%
GCTCTCAAACTTATATTCAA+TGG - chr2.3:68644254-68644273 MS.gene004322:intron 30.0%
GTGAAGAAGAAGAAAATAGT+TGG - chr2.3:68643926-68643945 MS.gene004322:CDS 30.0%
GTGACATGTTATTATGAGAA+AGG - chr2.3:68644845-68644864 MS.gene004322:CDS 30.0%
TAGTTGGAAGACCATTATTT+GGG - chr2.3:68643942-68643961 MS.gene004322:CDS 30.0%
TCCATATTCCTCTATATTCA+TGG + chr2.3:68644970-68644989 None:intergenic 30.0%
TCCTGGAATTTCTATTGTTT+GGG - chr2.3:68643382-68643401 MS.gene004322:intron 30.0%
TGTTGACAATTATAGACGAT+GGG - chr2.3:68643767-68643786 MS.gene004322:intron 30.0%
TTCCTGGAATTTCTATTGTT+TGG - chr2.3:68643381-68643400 MS.gene004322:intron 30.0%
TTCCTTATGTTCATCATTTC+TGG - chr2.3:68644356-68644375 MS.gene004322:intron 30.0%
TTGTTGACAATTATAGACGA+TGG - chr2.3:68643766-68643785 MS.gene004322:intron 30.0%
! ATTGCTTTAGATTGGTGAAT+TGG - chr2.3:68644685-68644704 MS.gene004322:intron 30.0%
!! AAGTCAATTTTATTTCGGCA+AGG + chr2.3:68644522-68644541 None:intergenic 30.0%
!! ATACTAAATGTGTTGTTTGC+GGG - chr2.3:68644734-68644753 MS.gene004322:CDS 30.0%
AAATAGGATCCTCAGAGTAA+TGG - chr2.3:68643731-68643750 MS.gene004322:intron 35.0%
AAGAGTTCTTCATTCCTCAA+AGG - chr2.3:68645082-68645101 MS.gene004322:CDS 35.0%
ACCAGCTTATATAATTGCTC+AGG - chr2.3:68644205-68644224 MS.gene004322:intron 35.0%
ACCTGAGCAATTATATAAGC+TGG + chr2.3:68644209-68644228 None:intergenic 35.0%
AGAGAGATAGACACATATTG+TGG - chr2.3:68644081-68644100 MS.gene004322:intron 35.0%
AGATAGACACATATTGTGGA+AGG - chr2.3:68644085-68644104 MS.gene004322:intron 35.0%
ATTGTGCACCATGAATATAG+AGG - chr2.3:68644959-68644978 MS.gene004322:intron 35.0%
CAATGTAGTAGCAAAATGGA+AGG - chr2.3:68643867-68643886 MS.gene004322:CDS 35.0%
CCTGGAATTTCTATTGTTTG+GGG - chr2.3:68643383-68643402 MS.gene004322:intron 35.0%
CTCCAGAAATGATGAACATA+AGG + chr2.3:68644361-68644380 None:intergenic 35.0%
CTTAAATTGTGGATGAGAGA+AGG + chr2.3:68644024-68644043 None:intergenic 35.0%
GTGGTTATGAATGTAAACGA+TGG - chr2.3:68643105-68643124 MS.gene004322:CDS 35.0%
GTTGACAATTATAGACGATG+GGG - chr2.3:68643768-68643787 MS.gene004322:intron 35.0%
TAGCAATGGAAATCTTGATG+TGG - chr2.3:68643086-68643105 MS.gene004322:CDS 35.0%
TATATAATTGCTCAGGTCGT+TGG - chr2.3:68644212-68644231 MS.gene004322:intron 35.0%
TATGTTGTGTCACCTATTCT+AGG - chr2.3:68644992-68645011 MS.gene004322:intron 35.0%
TATTCTATTGGCCATATCTC+TGG - chr2.3:68643428-68643447 MS.gene004322:intron 35.0%
TCAACATAAGCTTCGAACAT+AGG + chr2.3:68643968-68643987 None:intergenic 35.0%
TCGTTAAGTTATTTGATCGG+AGG - chr2.3:68643577-68643596 MS.gene004322:intron 35.0%
TGCTCAATGTAGTAGCAAAA+TGG - chr2.3:68643863-68643882 MS.gene004322:CDS 35.0%
TGGAAGAAGATGATCCAATT+CGG + chr2.3:68643991-68644010 None:intergenic 35.0%
TTTGAGGAATGAAGAACTCT+TGG + chr2.3:68645083-68645102 None:intergenic 35.0%
! AAAGTCACCTTTTGCAAAAG+AGG + chr2.3:68643174-68643193 None:intergenic 35.0%
! TACTTGATATTTGCTGGATG+TGG - chr2.3:68643317-68643336 MS.gene004322:intron 35.0%
! TCATGTTCCTCTTTTGCAAA+AGG - chr2.3:68643164-68643183 MS.gene004322:CDS 35.0%
! TTCTGATCTTCAAGCTTTTG+TGG - chr2.3:68644313-68644332 MS.gene004322:intron 35.0%
!! AATATAGTTGGTAGCAGAAG+TGG - chr2.3:68643283-68643302 MS.gene004322:intron 35.0%
!! GAATTATAGGGCATTGTGTT+TGG + chr2.3:68643526-68643545 None:intergenic 35.0%
!! TACTAAATGTGTTGTTTGCG+GGG - chr2.3:68644735-68644754 MS.gene004322:CDS 35.0%
AGACACATATTGTGGAAGGA+GGG - chr2.3:68644089-68644108 MS.gene004322:intron 40.0%
AGTTGCCACTGATAATAGAG+CGG - chr2.3:68644379-68644398 MS.gene004322:intron 40.0%
ATATTTGACAGACCCATCAC+AGG - chr2.3:68644899-68644918 MS.gene004322:intron 40.0%
ATCGGAGGTGATTAGAAGAA+CGG - chr2.3:68643592-68643611 MS.gene004322:intron 40.0%
ATGAATCCAGCAAGAAGCTT+AGG - chr2.3:68644929-68644948 MS.gene004322:intron 40.0%
ATTCATTGATGCTCCTGTGA+TGG + chr2.3:68644915-68644934 None:intergenic 40.0%
CATTGCATCTTGAATGCATG+CGG - chr2.3:68643806-68643825 MS.gene004322:CDS 40.0%
CCCCAAACAATAGAAATTCC+AGG + chr2.3:68643386-68643405 None:intergenic 40.0%
CTGGCTTATTAGTGATTCTG+AGG + chr2.3:68645048-68645067 None:intergenic 40.0%
CTTTAGATTGGTGAATTGGC+TGG - chr2.3:68644689-68644708 MS.gene004322:intron 40.0%
GAAGCTTATGTTGACCGAAT+TGG - chr2.3:68643974-68643993 MS.gene004322:CDS 40.0%
GAGCTTGTAGGTTGTTGAAA+CGG - chr2.3:68643623-68643642 MS.gene004322:intron 40.0%
GATAAAGTAGTGACACTTCC+TGG - chr2.3:68643365-68643384 MS.gene004322:intron 40.0%
GATTACCGCTCTATTATCAG+TGG + chr2.3:68644387-68644406 None:intergenic 40.0%
GGCAAGGAAAATCAATTTGC+AGG - chr2.3:68644275-68644294 MS.gene004322:intron 40.0%
TAGACACATATTGTGGAAGG+AGG - chr2.3:68644088-68644107 MS.gene004322:intron 40.0%
TATTCATGGTGCACAATAGC+TGG + chr2.3:68644956-68644975 None:intergenic 40.0%
TCGAACATAGGCCCAAATAA+TGG + chr2.3:68643956-68643975 None:intergenic 40.0%
TTCATTGATGCTCCTGTGAT+GGG + chr2.3:68644914-68644933 None:intergenic 40.0%
! AATGGAAGGTGTGAAGAGTA+TGG - chr2.3:68643881-68643900 MS.gene004322:CDS 40.0%
! AGTGATTCTGAGGAAAGTGT+AGG + chr2.3:68645038-68645057 None:intergenic 40.0%
! ATGGAAGGTGTGAAGAGTAT+GGG - chr2.3:68643882-68643901 MS.gene004322:CDS 40.0%
AGGATCCTCAGAGTAATGGT+TGG - chr2.3:68643735-68643754 MS.gene004322:intron 45.0%
ATTGTGGATGAGAGAAGGTG+AGG + chr2.3:68644019-68644038 None:intergenic 45.0%
CTTGTAGGTTGTTGAAACGG+CGG - chr2.3:68643626-68643645 MS.gene004322:intron 45.0%
GTTAGAAGATTGCACCTTGG+AGG - chr2.3:68643679-68643698 MS.gene004322:intron 45.0%
TTGTAGGTTGTTGAAACGGC+GGG - chr2.3:68643627-68643646 MS.gene004322:intron 45.0%
TTGTGGATGAGAGAAGGTGA+GGG + chr2.3:68644018-68644037 None:intergenic 45.0%
! ATTTGCTGGATGTGGTTCTG+TGG - chr2.3:68643325-68643344 MS.gene004322:intron 45.0%
! GTCACCTATTCTAGGAGCTT+TGG - chr2.3:68645000-68645019 MS.gene004322:intron 45.0%
! TCTTCATTCCTCAAAGGTGC+AGG - chr2.3:68645088-68645107 MS.gene004322:CDS 45.0%
!! AGGAGGCATTACAGACTTAC+GGG - chr2.3:68643829-68643848 MS.gene004322:CDS 45.0%
!! ATAGTTGGTAGCAGAAGTGG+TGG - chr2.3:68643286-68643305 MS.gene004322:intron 45.0%
!! GAGGAGGCATTACAGACTTA+CGG - chr2.3:68643828-68643847 MS.gene004322:CDS 45.0%
!! TAGTTGGTAGCAGAAGTGGT+GGG - chr2.3:68643287-68643306 MS.gene004322:intron 45.0%
!! TGTTTGGGGACTTGCTGTTA+TGG - chr2.3:68643397-68643416 MS.gene004322:intron 45.0%
ACGGCACTTGATGAGCTTGT+AGG - chr2.3:68643611-68643630 MS.gene004322:intron 50.0%
AGATTGCACCTTGGAGGACA+TGG - chr2.3:68643685-68643704 MS.gene004322:intron 50.0%
AGTACATCCCATGTCCTCCA+AGG + chr2.3:68643696-68643715 None:intergenic 50.0%
ATCTTGAATGCATGCGGAGG+AGG - chr2.3:68643812-68643831 MS.gene004322:CDS 50.0%
CCAGCCAAAGCTCCTAGAAT+AGG + chr2.3:68645007-68645026 None:intergenic 50.0%
CTGCTTCCATGGAAACCTTC+CGG + chr2.3:68643495-68643514 None:intergenic 50.0%
GAATTGGCTGGACTTGCAGT+TGG - chr2.3:68644701-68644720 MS.gene004322:CDS 50.0%
GATTGCACCTTGGAGGACAT+GGG - chr2.3:68643686-68643705 MS.gene004322:intron 50.0%
GCTCTCCAACCATTACTCTG+AGG + chr2.3:68643743-68643762 None:intergenic 50.0%
GCTTGAAGATCAGAACCAGC+AGG + chr2.3:68644308-68644327 None:intergenic 50.0%
GGGGTTAGAAGATTGCACCT+TGG - chr2.3:68643676-68643695 MS.gene004322:intron 50.0%
TGAGATGGGCACCAGAGATA+TGG + chr2.3:68643442-68643461 None:intergenic 50.0%
TGCATCTTGAATGCATGCGG+AGG - chr2.3:68643809-68643828 MS.gene004322:CDS 50.0%
TGGCGATTTACCTGCTTCCA+TGG + chr2.3:68643506-68643525 None:intergenic 50.0%
TGTAGGTTGTTGAAACGGCG+GGG - chr2.3:68643628-68643647 MS.gene004322:intron 50.0%
TTTGCAGGAACACTTCCTGC+TGG - chr2.3:68644290-68644309 MS.gene004322:intron 50.0%
! AAACCTTCCGGTGGTAGTGT+GGG + chr2.3:68643483-68643502 None:intergenic 50.0%
! CCTATTCTAGGAGCTTTGGC+TGG - chr2.3:68645004-68645023 MS.gene004322:intron 50.0%
! CTCAAAGGTGCAGGAAGCAA+GGG - chr2.3:68645097-68645116 MS.gene004322:CDS 50.0%
! GTTGGCTCAACACTTGCAAG+TGG - chr2.3:68644230-68644249 MS.gene004322:intron 50.0%
! TAGGAGCTTTGGCTGGTACA+TGG - chr2.3:68645011-68645030 MS.gene004322:intron 50.0%
!! GGAGGCATTACAGACTTACG+GGG - chr2.3:68643830-68643849 MS.gene004322:CDS 50.0%
ACCATTGCCCACACTACCAC+CGG - chr2.3:68643473-68643492 MS.gene004322:CDS 55.0%
AGTGTGGGCAATGGTGACAG+CGG + chr2.3:68643468-68643487 None:intergenic 55.0%
CCTTGCTTCCTGCACCTTTG+AGG + chr2.3:68645099-68645118 None:intergenic 55.0%
CGGAAGGTTTCCATGGAAGC+AGG - chr2.3:68643493-68643512 MS.gene004322:intron 55.0%
CTACCACCGGAAGGTTTCCA+TGG - chr2.3:68643486-68643505 MS.gene004322:CDS 55.0%
CTCTTGGTGAGCTCACGTAC+TGG + chr2.3:68645067-68645086 None:intergenic 55.0%
CTTCCATGGAAACCTTCCGG+TGG + chr2.3:68643492-68643511 None:intergenic 55.0%
GCTGGTCCTAAGCTTCTTGC+TGG + chr2.3:68644938-68644957 None:intergenic 55.0%
GGTGACAGCGGGATTGAGAT+GGG + chr2.3:68643456-68643475 None:intergenic 55.0%
TGAGAGAAGGTGAGGGTGAG+TGG + chr2.3:68644011-68644030 None:intergenic 55.0%
TGGTGACAGCGGGATTGAGA+TGG + chr2.3:68643457-68643476 None:intergenic 55.0%
! CCTCAAAGGTGCAGGAAGCA+AGG - chr2.3:68645096-68645115 MS.gene004322:CDS 55.0%
! GAAACCTTCCGGTGGTAGTG+TGG + chr2.3:68643484-68643503 None:intergenic 55.0%
! TGCTGGATGTGGTTCTGTGG+TGG - chr2.3:68643328-68643347 MS.gene004322:intron 55.0%
GTGTGGGCAATGGTGACAGC+GGG + chr2.3:68643467-68643486 None:intergenic 60.0%
TTGCCCACACTACCACCGGA+AGG - chr2.3:68643477-68643496 MS.gene004322:CDS 60.0%
! TCCGGTGGTAGTGTGGGCAA+TGG + chr2.3:68643477-68643496 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 68643075 68645129 68643075 ID=MS.gene004322
chr2.3 mRNA 68643075 68645129 68643075 ID=MS.gene004322.t1;Parent=MS.gene004322
chr2.3 exon 68645019 68645129 68645019 ID=MS.gene004322.t1.exon1;Parent=MS.gene004322.t1
chr2.3 CDS 68645019 68645129 68645019 ID=cds.MS.gene004322.t1;Parent=MS.gene004322.t1
chr2.3 exon 68644690 68644914 68644690 ID=MS.gene004322.t1.exon2;Parent=MS.gene004322.t1
chr2.3 CDS 68644690 68644914 68644690 ID=cds.MS.gene004322.t1;Parent=MS.gene004322.t1
chr2.3 exon 68643804 68644001 68643804 ID=MS.gene004322.t1.exon3;Parent=MS.gene004322.t1
chr2.3 CDS 68643804 68644001 68643804 ID=cds.MS.gene004322.t1;Parent=MS.gene004322.t1
chr2.3 exon 68643448 68643509 68643448 ID=MS.gene004322.t1.exon4;Parent=MS.gene004322.t1
chr2.3 CDS 68643448 68643509 68643448 ID=cds.MS.gene004322.t1;Parent=MS.gene004322.t1
chr2.3 exon 68643075 68643294 68643075 ID=MS.gene004322.t1.exon5;Parent=MS.gene004322.t1
chr2.3 CDS 68643075 68643294 68643075 ID=cds.MS.gene004322.t1;Parent=MS.gene004322.t1
Gene Sequence

>MS.gene004322

ATGGGTGATATTAGCAATGGAAATCTTGATGTGGTTATGAATGTAAACGATGGTGCAACAAAAAAATGTGACGACACAAGCATTGATGATCATGTTCCTCTTTTGCAAAAGGTGACTTTGTTTATCTTCCAAAAAAATTAATTGTTGTTTATGGTTAATTAATTAGATGTTCTGACTAAAAAAAATGGATGTTTTGCTTTGATTGTTGAATATAGTTGGTAGCAGAAGTGGTGGGAACATTCTACTTGATATTTGCTGGATGTGGTTCTGTGGTGGTGAACCTTAACAATGATAAAGTAGTGACACTTCCTGGAATTTCTATTGTTTGGGGACTTGCTGTTATGGTATTAGTATATTCTATTGGCCATATCTCTGGTGCCCATCTCAATCCCGCTGTCACCATTGCCCACACTACCACCGGAAGGTTTCCATGGAAGCAGGTAAATCGCCAAACACAATGCCCTATAATTCATATTAAGGCAAACATAATTTTCACAGCATCTCGTTAAGTTATTTGATCGGAGGTGATTAGAAGAACGGCACTTGATGAGCTTGTAGGTTGTTGAAACGGCGGGGTACCTCTAAAGAGTTTTGAATTTTTGGGGTTAGAAGATTGCACCTTGGAGGACATGGGATGTACTCTTATATAGTTGAGAAAATAGGATCCTCAGAGTAATGGTTGGAGAGCTGTTTGTTGACAATTATAGACGATGGGGTCTGTAACAGTCGAACATTGCATCTTGAATGCATGCGGAGGAGGCATTACAGACTTACGGGGTCTAAACAATTGCTCAATGTAGTAGCAAAATGGAAGGTGTGAAGAGTATGGGCGAAAGCGAGTTTGTGCCTATGTGAAGAAGAAGAAAATAGTTGGAAGACCATTATTTGGGCCTATGTTCGAAGCTTATGTTGACCGAATTGGATCATCTTCTTCCACTCACCCTCACCTTCTCTCATCCACAATTTAAGTCACTATCTCTCTCTTGATATTTTATCTAAATTTTAGAGAGAGATAGACACATATTGTGGAAGGAGGGAGAAAGAGAGAGTGTATGAAATGAGAGATCCTATAACGAAAACAACATTTTTAGGAACATTATTAACTTTGTACTTAATTTTTTCTTGCAGGTACCAGCTTATATAATTGCTCAGGTCGTTGGCTCAACACTTGCAAGTGGAGCTCTCAAACTTATATTCAATGGCAAGGAAAATCAATTTGCAGGAACACTTCCTGCTGGTTCTGATCTTCAAGCTTTTGTGGTTGAATTTATTATCACTTTCTTCCTTATGTTCATCATTTCTGGAGTTGCCACTGATAATAGAGCGGTAATCTTTTTTAAAAAAAAAATGCTTGGTTCATATAATATTAATTGATACTTATATTTTTATTAAACATTTTGATTAAGATTTAAGATATGCTAATAATTTATGAGATGAAAAATCACCTTGCCGAAATAAAATTGACTTTTTCTTTCAATAACAATAAAAAGTTCATTGATTAGATGTATAAATATATTCTAGGCTTATATAATTTTTTTTTTTGAGAGATTCTATGCTTATATAAATATTATTTAACCTTGTCTAATAGTTTTTGTATATTCATATTATTTATTGCTTTAGATTGGTGAATTGGCTGGACTTGCAGTTGGATCTACAGTTATACTAAATGTGTTGTTTGCGGGGTATTGATCTTAAAATTTTACCAAATATTATATCATAAAGTGCATATTTTGCACCAAGATTATACATTATATTTAATAAAATTAATATGTGACATGTTATTATGAGAAAGGTTTAAATAAAATTTAACTTGTGTGCGAACATATATTTGACAGACCCATCACAGGAGCATCAATGAATCCAGCAAGAAGCTTAGGACCAGCTATTGTGCACCATGAATATAGAGGAATATGGATATATGTTGTGTCACCTATTCTAGGAGCTTTGGCTGGTACATGGACCTACACTTTCCTCAGAATCACTAATAAGCCAGTACGTGAGCTCACCAAGAGTTCTTCATTCCTCAAAGGTGCAGGAAGCAAGGGAGCTGAGTGA
Protein sequence

>MS.gene004322.t1

MGDISNGNLDVVMNVNDGATKKCDDTSIDDHVPLLQKLVAEVVGTFYLIFAGCGSVVVNLNNDKVVTLPGISIVWGLAVMVLVYSIGHISGAHLNPAVTIAHTTTGRFPWKQVPAYIIAQVVGSTLASGALKLIFNGKENQFAGTLPAGSDLQAFVVEFIITFFLMFIISGVATDNRAIGELAGLAVGSTVILNVLFAGPITGASMNPARSLGPAIVHHEYRGIWIYVVSPILGALAGTWTYTFLRITNKPVRELTKSSSFLKGAGSKGAE