AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023621

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023621.t1 MTR_8g087710 98.188 276 5 0 1 276 1 276 0.0 550
MS.gene023621.t1 MTR_8g087720 73.585 265 70 0 4 268 3 267 7.63e-151 422
MS.gene023621.t1 MTR_2g017570 71.218 271 78 0 4 274 3 273 3.61e-144 405
MS.gene023621.t1 MTR_2g017610 71.218 271 78 0 4 274 3 273 2.30e-143 404
MS.gene023621.t1 MTR_2g017590 68.340 259 82 0 13 271 10 268 4.59e-135 382
MS.gene023621.t1 MTR_2g017660 64.706 272 89 2 4 268 3 274 1.20e-130 371
MS.gene023621.t1 MTR_2g017620 65.789 266 91 0 4 269 3 268 1.86e-128 365
MS.gene023621.t1 MTR_2g017620 67.323 254 83 0 16 269 1 254 5.99e-127 361
MS.gene023621.t1 MTR_4g084880 53.875 271 119 2 9 273 1 271 2.44e-106 310
MS.gene023621.t1 MTR_8g087720 72.864 199 54 0 4 202 3 201 5.37e-106 306
MS.gene023621.t1 MTR_2g017620 70.588 187 55 0 83 269 1 187 4.52e-95 278
MS.gene023621.t1 MTR_8g087720 67.513 197 63 1 4 200 3 198 6.26e-93 273
MS.gene023621.t1 MTR_5g079570 50.775 258 119 4 1 254 1 254 2.66e-85 256
MS.gene023621.t1 MTR_3g008920 53.556 239 108 2 19 257 8 243 3.29e-83 251
MS.gene023621.t1 MTR_4g006730 53.680 231 102 2 35 263 71 298 6.58e-78 238
MS.gene023621.t1 MTR_2g104940 52.863 227 102 2 39 263 80 303 4.45e-76 234
MS.gene023621.t1 MTR_7g010650 48.148 216 111 1 37 252 35 249 1.24e-72 224
MS.gene023621.t1 MTR_4g006730 53.738 214 94 2 52 263 1 211 4.05e-72 221
MS.gene023621.t1 MTR_4g006630 53.738 214 94 2 52 263 1 211 4.98e-72 221
MS.gene023621.t1 MTR_1g097840 49.362 235 108 3 34 260 69 300 2.47e-69 216
MS.gene023621.t1 MTR_5g063930 46.442 267 117 8 4 261 10 259 1.35e-66 208
MS.gene023621.t1 MTR_4g006730 48.259 201 87 3 35 233 71 256 3.82e-51 169
MS.gene023621.t1 MTR_1g097840 51.462 171 78 2 34 202 69 236 1.42e-46 157
MS.gene023621.t1 MTR_1g070385 40.271 221 131 1 33 253 56 275 2.35e-46 166
MS.gene023621.t1 MTR_7g010650 45.890 146 78 1 37 182 35 179 3.03e-40 138
MS.gene023621.t1 MTR_4g006650 50.694 144 57 3 40 181 5 136 3.11e-32 121
MS.gene023621.t1 MTR_8g013680 33.028 218 139 3 38 248 18 235 1.47e-26 104
MS.gene023621.t1 MTR_2g101370 32.645 242 145 7 34 264 15 249 3.00e-25 100
MS.gene023621.t1 MTR_5g012810 33.047 233 139 5 38 257 18 246 1.04e-24 99.4
MS.gene023621.t1 MTR_1g006490 31.120 241 145 8 13 246 3 229 2.38e-23 95.9
MS.gene023621.t1 MTR_1g077540 32.444 225 143 5 38 254 20 243 3.01e-23 95.9
MS.gene023621.t1 MTR_6g069040 32.159 227 141 5 38 254 20 243 8.05e-23 94.7
MS.gene023621.t1 MTR_1g006590 31.102 254 148 11 13 255 3 240 1.31e-22 94.0
MS.gene023621.t1 MTR_3g118010 31.222 221 137 5 37 242 35 255 1.53e-22 94.4
MS.gene023621.t1 MTR_7g103030 33.180 217 135 6 38 246 20 234 4.37e-21 89.7
MS.gene023621.t1 MTR_1g115405 32.759 232 142 7 38 257 20 249 7.39e-21 89.4
MS.gene023621.t1 MTR_2g094270 30.837 227 133 6 39 242 40 265 1.47e-20 89.4
MS.gene023621.t1 MTR_7g101190 30.357 224 133 6 37 238 37 259 4.42e-20 87.8
MS.gene023621.t1 MTR_4g063090 32.287 223 142 5 44 258 26 247 5.25e-20 87.0
MS.gene023621.t1 MTR_4g415300 28.409 264 164 8 1 248 26 280 1.06e-19 87.0
MS.gene023621.t1 MTR_8g098375 28.369 282 173 9 2 259 11 287 2.02e-19 85.9
MS.gene023621.t1 MTR_4g059390 30.568 229 134 7 37 242 37 263 1.01e-18 84.0
MS.gene023621.t1 MTR_5g010150 29.289 239 145 7 30 248 43 277 1.16e-18 84.0
MS.gene023621.t1 MTR_5g082070 29.707 239 145 7 37 256 53 287 1.28e-18 84.0
MS.gene023621.t1 MTR_3g070210 26.236 263 177 6 10 256 26 287 2.38e-18 83.2
MS.gene023621.t1 MTR_2g060360 30.045 223 148 4 38 253 18 239 1.48e-17 80.5
MS.gene023621.t1 MTR_2g094270 30.435 207 123 5 39 225 40 245 3.11e-17 79.7
MS.gene023621.t1 MTR_1g095070 30.220 182 109 4 82 248 25 203 1.37e-16 77.0
MS.gene023621.t1 MTR_3g070210 25.652 230 158 4 10 226 26 255 1.25e-14 72.4
MS.gene023621.t1 MTR_7g023350 27.706 231 160 3 38 261 20 250 2.37e-14 71.2
MS.gene023621.t1 MTR_1g095070 31.410 156 92 3 82 225 25 177 5.13e-14 69.3
MS.gene023621.t1 MTR_4g415300 28.177 181 122 5 30 203 47 226 8.88e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023621.t1 AT4G18910 71.486 249 64 1 33 274 46 294 3.05e-131 374
MS.gene023621.t1 AT4G19030 68.045 266 78 1 8 266 24 289 1.53e-128 367
MS.gene023621.t1 AT5G37820 60.000 235 94 0 39 273 43 277 2.31e-101 297
MS.gene023621.t1 AT5G37820 60.000 235 94 0 39 273 79 313 6.09e-101 298
MS.gene023621.t1 AT5G37810 53.333 270 119 2 3 266 2 270 6.03e-100 294
MS.gene023621.t1 AT1G31885 52.399 271 118 2 1 260 1 271 2.47e-98 291
MS.gene023621.t1 AT2G34390 53.101 258 113 2 17 267 26 282 7.17e-91 271
MS.gene023621.t1 AT4G18910 73.446 177 40 1 33 202 46 222 1.13e-89 265
MS.gene023621.t1 AT4G19030 65.672 201 62 1 8 201 24 224 6.28e-88 261
MS.gene023621.t1 AT1G31885 55.760 217 89 1 51 260 1 217 2.11e-86 259
MS.gene023621.t1 AT2G34390 53.782 238 102 2 37 267 4 240 2.53e-85 255
MS.gene023621.t1 AT4G19030 63.187 182 60 1 8 182 24 205 5.09e-76 231
MS.gene023621.t1 AT1G80760 50.820 244 113 3 12 253 55 293 3.09e-73 226
MS.gene023621.t1 AT4G10380 48.819 254 119 3 15 260 54 304 1.03e-72 225
MS.gene023621.t1 AT2G34390 55.959 193 77 2 17 202 26 217 1.40e-68 212
MS.gene023621.t1 AT2G34390 55.429 175 70 2 17 184 26 199 6.59e-62 195
MS.gene023621.t1 AT3G06100 38.057 247 150 3 10 254 16 261 7.56e-48 160
MS.gene023621.t1 AT3G06100 40.654 214 125 2 42 254 1 213 3.85e-47 157
MS.gene023621.t1 AT1G31885 48.936 141 65 2 1 134 1 141 1.55e-37 130
MS.gene023621.t1 AT3G16240 33.476 233 137 6 30 248 7 235 2.62e-29 112
MS.gene023621.t1 AT2G29870 52.688 93 44 0 154 246 20 112 7.25e-28 104
MS.gene023621.t1 AT5G47450 32.636 239 151 4 30 258 10 248 1.79e-27 107
MS.gene023621.t1 AT3G06100 38.298 141 85 2 115 254 41 180 2.98e-25 99.8
MS.gene023621.t1 AT4G17340 30.252 238 156 4 30 257 10 247 1.40e-24 99.4
MS.gene023621.t1 AT4G35100 31.579 228 141 5 30 242 29 256 2.33e-23 96.7
MS.gene023621.t1 AT4G35100 31.579 228 141 5 30 242 29 256 2.33e-23 96.7
MS.gene023621.t1 AT3G26520 33.486 218 138 3 38 248 21 238 7.32e-23 94.7
MS.gene023621.t1 AT1G17810 34.286 175 111 2 91 261 86 260 4.94e-22 92.8
MS.gene023621.t1 AT2G16850 30.769 221 138 5 37 242 34 254 5.01e-22 93.2
MS.gene023621.t1 AT2G36830 34.434 212 131 4 38 241 20 231 9.51e-22 91.7
MS.gene023621.t1 AT5G60660 29.362 235 143 6 36 248 36 269 6.31e-21 90.1
MS.gene023621.t1 AT2G39010 29.060 234 143 6 37 248 36 268 9.85e-21 89.7
MS.gene023621.t1 AT2G25810 32.420 219 135 7 38 248 18 231 2.80e-20 87.8
MS.gene023621.t1 AT1G73190 35.542 166 103 3 92 254 87 251 2.85e-20 88.2
MS.gene023621.t1 AT4G23400 28.629 248 155 6 30 259 44 287 6.72e-20 87.4
MS.gene023621.t1 AT2G37170 28.879 232 142 6 39 248 37 267 1.04e-19 87.0
MS.gene023621.t1 AT2G37170 28.879 232 142 6 39 248 80 310 1.22e-19 87.4
MS.gene023621.t1 AT3G61430 29.150 247 151 7 30 257 43 284 1.48e-19 86.3
MS.gene023621.t1 AT3G61430 29.150 247 151 7 30 257 43 284 1.48e-19 86.3
MS.gene023621.t1 AT4G01470 35.465 172 107 3 80 248 67 237 2.77e-19 85.1
MS.gene023621.t1 AT1G01620 29.412 238 145 5 30 248 43 276 2.94e-19 85.5
MS.gene023621.t1 AT3G54820 28.326 233 146 5 37 248 36 268 3.63e-19 85.5
MS.gene023621.t1 AT3G53420 28.755 233 145 6 37 248 37 269 4.28e-19 85.1
MS.gene023621.t1 AT3G53420 28.755 233 145 6 37 248 37 269 4.28e-19 85.1
MS.gene023621.t1 AT4G00430 27.509 269 170 6 2 248 12 277 4.45e-19 85.1
MS.gene023621.t1 AT2G45960 28.745 247 152 7 30 257 43 284 7.40e-19 84.3
MS.gene023621.t1 AT3G47440 29.224 219 145 4 38 248 22 238 2.82e-18 82.4
MS.gene023621.t1 AT2G37180 28.448 232 143 6 39 248 37 267 1.20e-17 81.3
MS.gene023621.t1 AT1G01620 31.720 186 107 4 69 238 13 194 1.43e-16 77.0
MS.gene023621.t1 AT2G45960 30.476 210 126 5 30 223 43 248 2.06e-16 77.4
MS.gene023621.t1 AT2G45960 30.476 210 126 5 30 223 43 248 2.12e-16 77.8
MS.gene023621.t1 AT2G39010 29.936 157 96 4 37 180 36 191 9.08e-12 63.5

Find 71 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTTATGTTTACCATTTC+TGG 0.053365 4.4:-13208310 MS.gene023621:CDS
ATATTTGTTGTCGCCGATTT+TGG 0.136028 4.4:-13207869 MS.gene023621:CDS
TTCCAATTTCTTAAACTATT+TGG 0.141022 4.4:+13207737 None:intergenic
TCCTGGGATTGCAATTGTTT+GGG 0.194809 4.4:-13209642 MS.gene023621:CDS
TTCATTGATGCTCCTGTTAC+CGG 0.292955 4.4:+13207949 None:intergenic
TTCCTGGGATTGCAATTGTT+TGG 0.304422 4.4:-13209643 MS.gene023621:CDS
AAGGAGGTTTCCATTGTTAC+AGG 0.315994 4.4:-13209531 MS.gene023621:intron
ACATATTTCTTGATATTTGC+AGG 0.316106 4.4:-13209713 MS.gene023621:CDS
CTCTGTCCCTTTCTTGCAAA+AGG 0.317695 4.4:-13209871 MS.gene023621:intron
CAAGAAAGGGACAGAGATAA+AGG 0.377304 4.4:+13209878 None:intergenic
GACAACGTGGTAACACTTCC+TGG 0.380447 4.4:-13209659 MS.gene023621:CDS
TACTCACTTGGTCATATCTC+TGG 0.390477 4.4:-13209596 MS.gene023621:CDS
CACCACGTCCAGCTTCTTTG+AGG 0.393717 4.4:+13207764 None:intergenic
TTATTTGACAGACCGGTAAC+AGG 0.396494 4.4:-13207961 MS.gene023621:intron
TTGCTTAATGTGATGATTGC+AGG 0.400582 4.4:-13208133 MS.gene023621:CDS
AATACAGTTAATAGCAGAAA+TGG 0.417887 4.4:-13209741 MS.gene023621:intron
GAGTTGGCTGGAATTGCAAT+TGG 0.418098 4.4:-13208166 MS.gene023621:CDS
TTGTAGATTGGTGAGTTGGC+TGG 0.424279 4.4:-13208178 MS.gene023621:intron
GCTGGTCCTAGAGTTCTAAC+CGG 0.437239 4.4:+13207925 None:intergenic
TTATATATCAGCTCAGCTCT+TGG 0.447897 4.4:-13208455 MS.gene023621:CDS
CATGGGTCTACAACACTGTT+AGG 0.451802 4.4:-13207831 MS.gene023621:CDS
CTTTGAGGAAGGATGCACTC+TGG 0.452465 4.4:+13207779 None:intergenic
ATTCAAGAACAAAAGCTTGA+AGG 0.459020 4.4:+13208347 None:intergenic
CTCCAGAAATGGTAAACATA+AGG 0.466289 4.4:+13208308 None:intergenic
ATCGGTGGCAACTCCAGAAA+TGG 0.467754 4.4:+13208297 None:intergenic
CCTGGGATTGCAATTGTTTG+GGG 0.472067 4.4:-13209641 MS.gene023621:CDS
TATATATCAGCTCAGCTCTT+GGG 0.473337 4.4:-13208454 MS.gene023621:CDS
TTTGAGGAAGGATGCACTCT+GGG 0.475411 4.4:+13207780 None:intergenic
AGTTGGCTGGAATTGCAATT+GGG 0.481359 4.4:-13208165 MS.gene023621:CDS
TGTTTGGGGACTAACTTTGT+TGG 0.484275 4.4:-13209627 MS.gene023621:CDS
GTAGACCCATGCTCCGGCGA+TGG 0.495881 4.4:+13207843 None:intergenic
TTGTATCTAAAACAACCTCA+TGG 0.499097 4.4:+13209937 None:intergenic
GCATCCTTCCTCAAAGAAGC+TGG 0.504133 4.4:-13207772 MS.gene023621:CDS
CCCCAAACAATTGCAATCCC+AGG 0.504953 4.4:+13209641 None:intergenic
TACTCATTGTGTACAAAAGC+TGG 0.505829 4.4:+13207907 None:intergenic
ACAATGAGTACAGAGGAATA+TGG 0.514676 4.4:-13207894 MS.gene023621:CDS
TCACAAACATGTGAAGGCTC+TGG 0.518719 4.4:-13209903 MS.gene023621:CDS
ATGAACCCGGTTAGAACTCT+AGG 0.519479 4.4:-13207931 MS.gene023621:CDS
TTTCAGGAACACTTCCATCT+GGG 0.526344 4.4:-13208375 MS.gene023621:CDS
TGTATCTAAAACAACCTCAT+GGG 0.536127 4.4:+13209938 None:intergenic
AGCTGAGCTGATATATAAGC+TGG 0.541389 4.4:+13208460 None:intergenic
CTCAAAGAAGCTGGACGTGG+TGG 0.543311 4.4:-13207763 MS.gene023621:CDS
ACGTCCAGCTTCTTTGAGGA+AGG 0.549185 4.4:+13207768 None:intergenic
GCAAAAGCAATAGTAACAGC+AGG 0.549342 4.4:+13209560 None:intergenic
TTCCTCAAAGAAGCTGGACG+TGG 0.550380 4.4:-13207766 MS.gene023621:CDS
TTGGTATTGATTTACTCACT+TGG 0.550812 4.4:-13209608 MS.gene023621:CDS
TGAGTTTACCGCTCTAGTAT+CGG 0.558198 4.4:+13208279 None:intergenic
CTCTGGGTGATCTCACGCAA+TGG 0.559604 4.4:+13207796 None:intergenic
CTGGTCCTAGAGTTCTAACC+GGG 0.561868 4.4:+13207926 None:intergenic
GGTAAACATAAGGTAAAATG+TGG 0.569076 4.4:+13208318 None:intergenic
ATGGAAGTGTTCCTGAAAAG+TGG 0.569487 4.4:+13208380 None:intergenic
TGCTTAATGTGATGATTGCA+GGG 0.570893 4.4:-13208132 MS.gene023621:intron
ACAACGTGGTAACACTTCCT+GGG 0.584721 4.4:-13209658 MS.gene023621:CDS
ACAGTTAATAGCAGAAATGG+TGG 0.598028 4.4:-13209738 MS.gene023621:intron
TCAAAGAAGCTGGACGTGGT+GGG 0.603447 4.4:-13207762 MS.gene023621:CDS
AGTGTTGTAGACCCATGCTC+CGG 0.603956 4.4:+13207837 None:intergenic
GCTTGAAGGTTAGACCCAGA+TGG 0.605252 4.4:+13208361 None:intergenic
TTGGGAGCAACACTTGCTAG+TGG 0.608245 4.4:-13208436 MS.gene023621:CDS
ACTCTTAAACTAATATTCAG+TGG 0.615140 4.4:-13208412 MS.gene023621:CDS
GATTCCTCACAAACATGTGA+AGG 0.617028 4.4:-13209909 MS.gene023621:CDS
GTAGTGTTGACCTGTAACAA+TGG 0.623661 4.4:+13209521 None:intergenic
TGCTTCAATAGTAGTGAACA+AGG 0.624250 4.4:-13209687 MS.gene023621:CDS
AACAGGAGCATCAATGAACC+CGG 0.626218 4.4:-13207944 MS.gene023621:CDS
AGAGCCTTCACATGTTTGTG+AGG 0.626868 4.4:+13209905 None:intergenic
CAGTTAATAGCAGAAATGGT+GGG 0.636357 4.4:-13209737 MS.gene023621:CDS
GTTTACCGCTCTAGTATCGG+TGG 0.679010 4.4:+13208282 None:intergenic
AGCAAGAATTGAAACCCATG+AGG 0.683901 4.4:-13209952 MS.gene023621:CDS
TTTGTACACAATGAGTACAG+AGG 0.696529 4.4:-13207901 MS.gene023621:CDS
TAACAATGGAAACCTCCTTG+TGG 0.700560 4.4:+13209535 None:intergenic
AGTTGCCACCGATACTAGAG+CGG 0.735236 4.4:-13208287 MS.gene023621:intron
GAACAAGGACAACGACAACG+TGG 0.744853 4.4:-13209672 MS.gene023621:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAAATATATATAAAAT+TGG - chr4.4:13208913-13208932 MS.gene023621:intron 0.0%
!! ATTTACAAAAATAAACTTTA+TGG + chr4.4:13208237-13208256 None:intergenic 10.0%
!! TATAGTATATTGAATTAATT+AGG - chr4.4:13207936-13207955 MS.gene023621:CDS 10.0%
!!! AAAAAAGATTATTTTTTTAC+AGG + chr4.4:13208505-13208524 None:intergenic 10.0%
!!! AAACTATTTTTTTTTAATCT+TGG - chr4.4:13208344-13208363 MS.gene023621:CDS 10.0%
!!! TATTTAGAATTTACTATTAT+TGG + chr4.4:13208889-13208908 None:intergenic 10.0%
!!! TTTTTTTGACAAAAAAATTA+TGG - chr4.4:13208966-13208985 MS.gene023621:intron 10.0%
!! TACAAATTGAGTTAAAAAAA+GGG + chr4.4:13208466-13208485 None:intergenic 15.0%
!! TTACAAATTGAGTTAAAAAA+AGG + chr4.4:13208467-13208486 None:intergenic 15.0%
!!! AGTTTTGCAATTTTTTTTTA+TGG + chr4.4:13209662-13209681 None:intergenic 15.0%
!!! ATATTTTATGTTTTGTAGAT+TGG - chr4.4:13209526-13209545 MS.gene023621:intron 15.0%
!!! ATTGTGGTAAAAATAATTTT+TGG + chr4.4:13209193-13209212 None:intergenic 15.0%
!!! TGTAACTTTTTATTAAATGA+TGG + chr4.4:13208595-13208614 None:intergenic 15.0%
!!! TTTGATAACCTTTAAAATTT+GGG + chr4.4:13208767-13208786 None:intergenic 15.0%
!!! TTTTGATAACCTTTAAAATT+TGG + chr4.4:13208768-13208787 None:intergenic 15.0%
!! ACAAATTGAGTTAAAAAAAG+GGG + chr4.4:13208465-13208484 None:intergenic 20.0%
!! ACAATATATAGAATAACAAG+TGG - chr4.4:13208675-13208694 MS.gene023621:intron 20.0%
!! AGATAATTATACCGAAAAAA+TGG + chr4.4:13208382-13208401 None:intergenic 20.0%
!! ATCCTTTGTATAATTCTTAT+AGG - chr4.4:13209231-13209250 MS.gene023621:intron 20.0%
!! CCAAAATTCTTCAATTTATT+AGG - chr4.4:13209085-13209104 MS.gene023621:intron 20.0%
!! TACCTATAAGAATTATACAA+AGG + chr4.4:13209236-13209255 None:intergenic 20.0%
!! TTTATTAAATGATGGTAAGT+TGG + chr4.4:13208587-13208606 None:intergenic 20.0%
!!! CCTAATAAATTGAAGAATTT+TGG + chr4.4:13209088-13209107 None:intergenic 20.0%
!!! GACTTAAAAGTTTTGTTTTT+TGG + chr4.4:13208534-13208553 None:intergenic 20.0%
!!! TAGTAATTTTGCATTTAACT+AGG + chr4.4:13209632-13209651 None:intergenic 20.0%
!!! TGATATTTTCATGTAAAATC+AGG + chr4.4:13208838-13208857 None:intergenic 20.0%
!!! TTAATTAGGTTTTGTTTTGT+TGG - chr4.4:13207950-13207969 MS.gene023621:CDS 20.0%
!!! TTGGTTTAAAATTAATGTCA+AGG + chr4.4:13208870-13208889 None:intergenic 20.0%
! AAAGAGTCTTAATTTCTTGT+GGG + chr4.4:13208410-13208429 None:intergenic 25.0%
! AAATTGAGTTAAAAAAAGGG+GGG + chr4.4:13208463-13208482 None:intergenic 25.0%
! AACTAAGTAACATGATTTCT+TGG + chr4.4:13209167-13209186 None:intergenic 25.0%
! AATACAGTTAATAGCAGAAA+TGG - chr4.4:13207975-13207994 MS.gene023621:intron 25.0%
! ACATATTTCTTGATATTTGC+AGG - chr4.4:13208003-13208022 MS.gene023621:intron 25.0%
! ACTCTTAAACTAATATTCAG+TGG - chr4.4:13209304-13209323 MS.gene023621:intron 25.0%
! CAAATTGAGTTAAAAAAAGG+GGG + chr4.4:13208464-13208483 None:intergenic 25.0%
! TAAAGAGTCTTAATTTCTTG+TGG + chr4.4:13208411-13208430 None:intergenic 25.0%
! TGATATTTCTTAAAGCACAT+GGG - chr4.4:13209003-13209022 MS.gene023621:intron 25.0%
! TTCATAATTGCATAACATTG+TGG + chr4.4:13209209-13209228 None:intergenic 25.0%
!! ATTCAACGAGTATTTTCATT+TGG + chr4.4:13209125-13209144 None:intergenic 25.0%
!! TAATCCAACCCAAATTTTAA+AGG - chr4.4:13208756-13208775 MS.gene023621:intron 25.0%
!! TTCAACGAGTATTTTCATTT+GGG + chr4.4:13209124-13209143 None:intergenic 25.0%
!! TTTTCAATTTCAAGAGAAGT+GGG + chr4.4:13208437-13208456 None:intergenic 25.0%
!!! ATAACCTTTAAAATTTGGGT+TGG + chr4.4:13208763-13208782 None:intergenic 25.0%
!!! ATTTAACTAGGTTCTAGATA+GGG + chr4.4:13209620-13209639 None:intergenic 25.0%
!!! TTCTTGATTAGCCATTTTTT+CGG - chr4.4:13208368-13208387 MS.gene023621:CDS 25.0%
AAAATTGGCATAACTCTCAT+TGG - chr4.4:13208928-13208947 MS.gene023621:intron 30.0%
ATTCAAGAACAAAAGCTTGA+AGG + chr4.4:13209372-13209391 None:intergenic 30.0%
CTATGTGTATGAGTTTGTAA+AGG + chr4.4:13207886-13207905 None:intergenic 30.0%
GACGATATCACTTTACAATT+CGG - chr4.4:13208716-13208735 MS.gene023621:intron 30.0%
GAGTTTGTAAAGGAAAGATT+AGG + chr4.4:13207876-13207895 None:intergenic 30.0%
GGTAAACATAAGGTAAAATG+TGG + chr4.4:13209401-13209420 None:intergenic 30.0%
GTGATATTTCTTAAAGCACA+TGG - chr4.4:13209002-13209021 MS.gene023621:intron 30.0%
TACCTTATGTTTACCATTTC+TGG - chr4.4:13209406-13209425 MS.gene023621:intron 30.0%
! GTTTTCAATTTCAAGAGAAG+TGG + chr4.4:13208438-13208457 None:intergenic 30.0%
! TGTATCTAAAACAACCTCAT+GGG + chr4.4:13207781-13207800 None:intergenic 30.0%
! TTGTATCTAAAACAACCTCA+TGG + chr4.4:13207782-13207801 None:intergenic 30.0%
! TTTTCATTTGGGAGCTTTTA+CGG + chr4.4:13209113-13209132 None:intergenic 30.0%
! TTTTCGGTATAATTATCTGC+AGG - chr4.4:13208384-13208403 MS.gene023621:CDS 30.0%
!! ACTTTACAATTCGGTTTTGT+AGG - chr4.4:13208725-13208744 MS.gene023621:intron 30.0%
!! CATTTAACTAGGTTCTAGAT+AGG + chr4.4:13209621-13209640 None:intergenic 30.0%
!! CTTTACAATTCGGTTTTGTA+GGG - chr4.4:13208726-13208745 MS.gene023621:intron 30.0%
!! TTGGTATTGATTTACTCACT+TGG - chr4.4:13208108-13208127 MS.gene023621:intron 30.0%
!!! CACTTTTTTATTTGACAGAC+CGG - chr4.4:13209748-13209767 MS.gene023621:intron 30.0%
!!! GGTTGTTTTAGATACAAACA+AGG - chr4.4:13207785-13207804 MS.gene023621:CDS 30.0%
ACAATGAGTACAGAGGAATA+TGG - chr4.4:13209822-13209841 MS.gene023621:intron 35.0%
ACAGTTAATAGCAGAAATGG+TGG - chr4.4:13207978-13207997 MS.gene023621:intron 35.0%
CAGTTAATAGCAGAAATGGT+GGG - chr4.4:13207979-13207998 MS.gene023621:intron 35.0%
CTCCAGAAATGGTAAACATA+AGG + chr4.4:13209411-13209430 None:intergenic 35.0%
GTGGTTTGATTCATTACTCT+CGG - chr4.4:13208694-13208713 MS.gene023621:intron 35.0%
TACTCATTGTGTACAAAAGC+TGG + chr4.4:13209812-13209831 None:intergenic 35.0%
TATATATCAGCTCAGCTCTT+GGG - chr4.4:13209262-13209281 MS.gene023621:intron 35.0%
TGCTTAATGTGATGATTGCA+GGG - chr4.4:13209584-13209603 MS.gene023621:CDS 35.0%
TGCTTCAATAGTAGTGAACA+AGG - chr4.4:13208029-13208048 MS.gene023621:intron 35.0%
TTATATATCAGCTCAGCTCT+TGG - chr4.4:13209261-13209280 MS.gene023621:intron 35.0%
TTGCTTAATGTGATGATTGC+AGG - chr4.4:13209583-13209602 MS.gene023621:CDS 35.0%
TTTGTACACAATGAGTACAG+AGG - chr4.4:13209815-13209834 MS.gene023621:intron 35.0%
! TGTTTTGTAGATTGGTGAGT+TGG - chr4.4:13209534-13209553 MS.gene023621:CDS 35.0%
!!! ATATTTGTTGTCGCCGATTT+TGG - chr4.4:13209847-13209866 MS.gene023621:intron 35.0%
!!! TATTTGTTGTCGCCGATTTT+GGG - chr4.4:13209848-13209867 MS.gene023621:intron 35.0%
AAGGAGGTTTCCATTGTTAC+AGG - chr4.4:13208185-13208204 MS.gene023621:intron 40.0%
AGCAAGAATTGAAACCCATG+AGG - chr4.4:13207764-13207783 MS.gene023621:CDS 40.0%
AGCTGAGCTGATATATAAGC+TGG + chr4.4:13209259-13209278 None:intergenic 40.0%
AGTTGGCTGGAATTGCAATT+GGG - chr4.4:13209551-13209570 MS.gene023621:CDS 40.0%
CAAGAAAGGGACAGAGATAA+AGG + chr4.4:13207841-13207860 None:intergenic 40.0%
GATTCCTCACAAACATGTGA+AGG - chr4.4:13207807-13207826 MS.gene023621:CDS 40.0%
GCAAAAGCAATAGTAACAGC+AGG + chr4.4:13208159-13208178 None:intergenic 40.0%
TAACAATGGAAACCTCCTTG+TGG + chr4.4:13208184-13208203 None:intergenic 40.0%
TACTCACTTGGTCATATCTC+TGG - chr4.4:13208120-13208139 MS.gene023621:intron 40.0%
TCCTGGGATTGCAATTGTTT+GGG - chr4.4:13208074-13208093 MS.gene023621:intron 40.0%
TGAGTTTACCGCTCTAGTAT+CGG + chr4.4:13209440-13209459 None:intergenic 40.0%
TGTTTGGGGACTAACTTTGT+TGG - chr4.4:13208089-13208108 MS.gene023621:intron 40.0%
TTATTTGACAGACCGGTAAC+AGG - chr4.4:13209755-13209774 MS.gene023621:intron 40.0%
TTCATTGATGCTCCTGTTAC+CGG + chr4.4:13209770-13209789 None:intergenic 40.0%
TTCCTGGGATTGCAATTGTT+TGG - chr4.4:13208073-13208092 MS.gene023621:intron 40.0%
TTTCAGGAACACTTCCATCT+GGG - chr4.4:13209341-13209360 MS.gene023621:intron 40.0%
! GAGACACCTTTTGCAAGAAA+GGG + chr4.4:13207854-13207873 None:intergenic 40.0%
!! ATGGAAGTGTTCCTGAAAAG+TGG + chr4.4:13209339-13209358 None:intergenic 40.0%
!! CTATTGCTTTTGCTACCACA+AGG - chr4.4:13208166-13208185 MS.gene023621:CDS 40.0%
!! GTAGTGTTGACCTGTAACAA+TGG + chr4.4:13208198-13208217 None:intergenic 40.0%
!! TTTTCAGGAACACTTCCATC+TGG - chr4.4:13209340-13209359 MS.gene023621:intron 40.0%
!!! ATTTGTTGTCGCCGATTTTG+GGG - chr4.4:13209849-13209868 MS.gene023621:intron 40.0%
ACAACGTGGTAACACTTCCT+GGG - chr4.4:13208058-13208077 MS.gene023621:intron 45.0%
AGAGCCTTCACATGTTTGTG+AGG + chr4.4:13207814-13207833 None:intergenic 45.0%
ATGAACCCGGTTAGAACTCT+AGG - chr4.4:13209785-13209804 MS.gene023621:intron 45.0%
CATGGGTCTACAACACTGTT+AGG - chr4.4:13209885-13209904 MS.gene023621:CDS 45.0%
CCTGGGATTGCAATTGTTTG+GGG - chr4.4:13208075-13208094 MS.gene023621:intron 45.0%
CTCTGTCCCTTTCTTGCAAA+AGG - chr4.4:13207845-13207864 MS.gene023621:CDS 45.0%
GAGTTGGCTGGAATTGCAAT+TGG - chr4.4:13209550-13209569 MS.gene023621:CDS 45.0%
TCACAAACATGTGAAGGCTC+TGG - chr4.4:13207813-13207832 MS.gene023621:CDS 45.0%
TTTGAGGAAGGATGCACTCT+GGG + chr4.4:13209939-13209958 None:intergenic 45.0%
! GGAGACACCTTTTGCAAGAA+AGG + chr4.4:13207855-13207874 None:intergenic 45.0%
! TTGTAGATTGGTGAGTTGGC+TGG - chr4.4:13209538-13209557 MS.gene023621:CDS 45.0%
!! AACAGGAGCATCAATGAACC+CGG - chr4.4:13209772-13209791 MS.gene023621:intron 45.0%
!! TTGCTTTTGCTACCACAAGG+AGG - chr4.4:13208169-13208188 MS.gene023621:CDS 45.0%
!!! TTTGTTGTCGCCGATTTTGG+GGG - chr4.4:13209850-13209869 MS.gene023621:intron 45.0%
AGTGTTGTAGACCCATGCTC+CGG + chr4.4:13209882-13209901 None:intergenic 50.0%
AGTTGCCACCGATACTAGAG+CGG - chr4.4:13209429-13209448 MS.gene023621:intron 50.0%
ATCGGTGGCAACTCCAGAAA+TGG + chr4.4:13209422-13209441 None:intergenic 50.0%
CCCCAAACAATTGCAATCCC+AGG + chr4.4:13208078-13208097 None:intergenic 50.0%
GAACAAGGACAACGACAACG+TGG - chr4.4:13208044-13208063 MS.gene023621:intron 50.0%
GACAACGTGGTAACACTTCC+TGG - chr4.4:13208057-13208076 MS.gene023621:intron 50.0%
GCATCCTTCCTCAAAGAAGC+TGG - chr4.4:13209944-13209963 MS.gene023621:CDS 50.0%
GCTTGAAGGTTAGACCCAGA+TGG + chr4.4:13209358-13209377 None:intergenic 50.0%
TCAAAGAAGCTGGACGTGGT+GGG - chr4.4:13209954-13209973 MS.gene023621:CDS 50.0%
TTCCTCAAAGAAGCTGGACG+TGG - chr4.4:13209950-13209969 MS.gene023621:CDS 50.0%
! ACGTCCAGCTTCTTTGAGGA+AGG + chr4.4:13209951-13209970 None:intergenic 50.0%
! CTGGTCCTAGAGTTCTAACC+GGG + chr4.4:13209793-13209812 None:intergenic 50.0%
! CTTTGAGGAAGGATGCACTC+TGG + chr4.4:13209940-13209959 None:intergenic 50.0%
! GCTGGTCCTAGAGTTCTAAC+CGG + chr4.4:13209794-13209813 None:intergenic 50.0%
! GTTTACCGCTCTAGTATCGG+TGG + chr4.4:13209437-13209456 None:intergenic 50.0%
! TTGGGAGCAACACTTGCTAG+TGG - chr4.4:13209280-13209299 MS.gene023621:intron 50.0%
!! GGCACACATGACCACTTTTC+AGG - chr4.4:13209325-13209344 MS.gene023621:intron 50.0%
CACCACGTCCAGCTTCTTTG+AGG + chr4.4:13209955-13209974 None:intergenic 55.0%
CTCAAAGAAGCTGGACGTGG+TGG - chr4.4:13209953-13209972 MS.gene023621:CDS 55.0%
CTCTGGGTGATCTCACGCAA+TGG + chr4.4:13209923-13209942 None:intergenic 55.0%
CCGGCGATGGCCCCCAAAAT+CGG + chr4.4:13209863-13209882 None:intergenic 65.0%
GTAGACCCATGCTCCGGCGA+TGG + chr4.4:13209876-13209895 None:intergenic 65.0%
!! CCGATTTTGGGGGCCATCGC+CGG - chr4.4:13209860-13209879 MS.gene023621:intron 65.0%
GGGGGCCATCGCCGGAGCAT+GGG - chr4.4:13209868-13209887 MS.gene023621:intron 75.0%
TGGGGGCCATCGCCGGAGCA+TGG - chr4.4:13209867-13209886 MS.gene023621:intron 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 13207747 13209991 13207747 ID=MS.gene023621
chr4.4 mRNA 13207747 13209991 13207747 ID=MS.gene023621.t1;Parent=MS.gene023621
chr4.4 exon 13209872 13209991 13209872 ID=MS.gene023621.t1.exon1;Parent=MS.gene023621.t1
chr4.4 CDS 13209872 13209991 13209872 ID=cds.MS.gene023621.t1;Parent=MS.gene023621.t1
chr4.4 exon 13209532 13209756 13209532 ID=MS.gene023621.t1.exon2;Parent=MS.gene023621.t1
chr4.4 CDS 13209532 13209756 13209532 ID=cds.MS.gene023621.t1;Parent=MS.gene023621.t1
chr4.4 exon 13208288 13208485 13208288 ID=MS.gene023621.t1.exon3;Parent=MS.gene023621.t1
chr4.4 CDS 13208288 13208485 13208288 ID=cds.MS.gene023621.t1;Parent=MS.gene023621.t1
chr4.4 exon 13208133 13208194 13208133 ID=MS.gene023621.t1.exon4;Parent=MS.gene023621.t1
chr4.4 CDS 13208133 13208194 13208133 ID=cds.MS.gene023621.t1;Parent=MS.gene023621.t1
chr4.4 exon 13207747 13207972 13207747 ID=MS.gene023621.t1.exon5;Parent=MS.gene023621.t1
chr4.4 CDS 13207747 13207972 13207747 ID=cds.MS.gene023621.t1;Parent=MS.gene023621.t1
Gene Sequence

>MS.gene023621

ATGGCAAATGATAATTCAGCAAGAATTGAAACCCATGAGGTTGTTTTAGATACAAACAAGGATTCCTCACAAACATGTGAAGGCTCTGGCTCCTTTATCTCTGTCCCTTTCTTGCAAAAGGTGTCTCCTAATCTTTCCTTTACAAACTCATACACATAGTTACATAGTTTCTTTCTGAACTTTTGATCATATAGTATATTGAATTAATTAGGTTTTGTTTTGTTGGTGAATACAGTTAATAGCAGAAATGGTGGGCACATATTTCTTGATATTTGCAGGATGTGCTTCAATAGTAGTGAACAAGGACAACGACAACGTGGTAACACTTCCTGGGATTGCAATTGTTTGGGGACTAACTTTGTTGGTATTGATTTACTCACTTGGTCATATCTCTGGTGCTCATTTCAATCCTGCTGTTACTATTGCTTTTGCTACCACAAGGAGGTTTCCATTGTTACAGGTCAACACTACACACAATCTTTTTTCTCCATAAAGTTTATTTTTGTAAATAATATAATATTTTGACATACAAAGTTGATAACATATATACTCTATTTACTTTATTTAAATGTATATATATACTCTATAATACAAGATAAACTATTTTTTTTTAATCTTGGCTTCTTGATTAGCCATTTTTTCGGTATAATTATCTGCAGGCCCACAAGAAATTAAGACTCTTTAAAGCCCACTTCTCTTGAAATTGAAAACTACCCCCCTTTTTTTAACTCAATTTGTAAAAACAAGTATGTTATCCTGTAAAAAAATAATCTTTTTTTTTTTACCAAAAAACAAAACTTTTAAGTCTGAATATATTTTCTCTTCGTTGTTTTCACGCCAACTTACCATCATTTAATAAAAAGTTACACGATATCATTTTCAAAATGTAATAATAACTAAATTATATATTGAAAATGATAAAATAGAGACAATATATAGAATAACAAGTGGTTTGATTCATTACTCTCGGACGATATCACTTTACAATTCGGTTTTGTAGGGTAGTGCTTAATCCAACCCAAATTTTAAAGGTTATCAAAACATTAAAAGTATAAGAAAACAAAATTCATAAGTCAATACTTTGTATTCCTGATTTTACATGAAAATATCAGTCAAATAACCTTGACATTAATTTTAAACCAATAATAGTAAATTCTAAATATTTTTAAATAAATATATATAAAATTGGCATAACTCTCATTGGTAATAAAATGATGTTTTTTTTTGACAAAAAAATTATGGTAATTTTTATGAAGTGATATTTCTTAAAGCACATGGGTTTACAAATAGTTCTTTGATTAAAAAACATTCTACTATTTATTGATTGAGAAAATTATACCAAAATTCTTCAATTTATTAGGTACCGTAAAAGCTCCCAAATGAAAATACTCGTTGAATAAGTAGCTAAAATGTTTCTCCAAGAAATCATGTTACTTAGTTTGTCCAAAAATTATTTTTACCACAATGTTATGCAATTATGAATCATCCTTTGTATAATTCTTATAGGTACCAGCTTATATATCAGCTCAGCTCTTGGGAGCAACACTTGCTAGTGGAACTCTTAAACTAATATTCAGTGGCACACATGACCACTTTTCAGGAACACTTCCATCTGGGTCTAACCTTCAAGCTTTTGTTCTTGAATTCATAACCACATTTTACCTTATGTTTACCATTTCTGGAGTTGCCACCGATACTAGAGCGGTAAACTCATCAAACACATCGATCTCATGTTCATATAAACGTTCTTTAATACTTTTATTCATTATTTTTATTTTCATATTTTATGTTTTGTAGATTGGTGAGTTGGCTGGAATTGCAATTGGGTCTACATTATTGCTTAATGTGATGATTGCAGGGTATGTTTCTACCCTATCTAGAACCTAGTTAAATGCAAAATTACTAATATGTTCCATAAAAAAAAATTGCAAAACTAAGAGTTAGATATTTATGTCGAATCTAATTTATCTGTATAACACGAATTTGTAAAATGAGACGTGTCACTTTTTTATTTGACAGACCGGTAACAGGAGCATCAATGAACCCGGTTAGAACTCTAGGACCAGCTTTTGTACACAATGAGTACAGAGGAATATGGATATATTTGTTGTCGCCGATTTTGGGGGCCATCGCCGGAGCATGGGTCTACAACACTGTTAGGTACACTAACAAGCCATTGCGTGAGATCACCCAGAGTGCATCCTTCCTCAAAGAAGCTGGACGTGGTGGGAACCAAATAGTTTAA
Protein sequence

>MS.gene023621.t1

MANDNSARIETHEVVLDTNKDSSQTCEGSGSFISVPFLQKLIAEMVGTYFLIFAGCASIVVNKDNDNVVTLPGIAIVWGLTLLVLIYSLGHISGAHFNPAVTIAFATTRRFPLLQVPAYISAQLLGATLASGTLKLIFSGTHDHFSGTLPSGSNLQAFVLEFITTFYLMFTISGVATDTRAIGELAGIAIGSTLLLNVMIAGPVTGASMNPVRTLGPAFVHNEYRGIWIYLLSPILGAIAGAWVYNTVRYTNKPLREITQSASFLKEAGRGGNQIV