AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene03090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03090.t1 MTR_2g017590 92.251 271 20 1 1 271 1 270 0.0 507
MS.gene03090.t1 MTR_2g017620 93.680 269 15 1 1 267 1 269 0.0 506
MS.gene03090.t1 MTR_2g017620 94.902 255 13 0 13 267 1 255 2.58e-178 491
MS.gene03090.t1 MTR_2g017570 79.401 267 55 0 5 271 7 273 1.76e-159 444
MS.gene03090.t1 MTR_2g017610 79.401 267 55 0 5 271 7 273 2.34e-159 444
MS.gene03090.t1 MTR_8g087720 74.231 260 67 0 5 264 7 266 2.26e-144 405
MS.gene03090.t1 MTR_8g087710 67.176 262 86 0 10 271 13 274 2.25e-130 370
MS.gene03090.t1 MTR_2g017620 95.745 188 8 0 80 267 1 188 1.26e-129 365
MS.gene03090.t1 MTR_2g017660 65.074 272 86 2 1 263 1 272 6.83e-129 367
MS.gene03090.t1 MTR_8g087720 73.846 195 51 0 5 199 7 201 6.90e-102 295
MS.gene03090.t1 MTR_4g084880 57.627 236 98 1 32 265 31 266 1.23e-97 287
MS.gene03090.t1 MTR_8g087720 71.348 178 51 0 5 182 7 184 1.35e-88 262
MS.gene03090.t1 MTR_5g079570 52.610 249 109 3 6 251 12 254 4.56e-88 263
MS.gene03090.t1 MTR_3g008920 53.138 239 109 2 16 254 8 243 4.57e-85 255
MS.gene03090.t1 MTR_4g006730 49.799 249 118 2 15 260 54 298 1.04e-73 228
MS.gene03090.t1 MTR_2g104940 49.206 252 119 3 14 260 56 303 9.27e-72 223
MS.gene03090.t1 MTR_7g010650 47.137 227 118 2 36 262 37 261 1.15e-69 216
MS.gene03090.t1 MTR_1g097840 49.573 234 107 3 32 257 70 300 3.42e-67 211
MS.gene03090.t1 MTR_5g063930 44.737 266 132 6 1 258 1 259 6.16e-67 209
MS.gene03090.t1 MTR_4g006630 52.093 215 96 2 49 260 1 211 1.28e-66 206
MS.gene03090.t1 MTR_4g006730 52.093 215 96 2 49 260 1 211 1.66e-66 206
MS.gene03090.t1 MTR_1g070385 43.192 213 120 1 38 250 64 275 2.86e-49 174
MS.gene03090.t1 MTR_4g006730 43.836 219 104 3 14 229 53 255 3.29e-47 158
MS.gene03090.t1 MTR_1g097840 52.047 171 75 2 32 199 70 236 7.67e-44 150
MS.gene03090.t1 MTR_7g010650 41.667 144 83 1 36 179 37 179 2.94e-35 125
MS.gene03090.t1 MTR_1g006490 39.336 211 118 7 35 238 18 225 3.93e-29 111
MS.gene03090.t1 MTR_8g013680 32.773 238 149 6 28 254 9 246 3.46e-28 108
MS.gene03090.t1 MTR_2g101370 33.061 245 152 6 19 254 6 247 2.03e-27 106
MS.gene03090.t1 MTR_6g069040 32.099 243 154 6 19 251 2 243 3.98e-27 105
MS.gene03090.t1 MTR_1g077540 31.818 242 156 4 19 251 2 243 5.39e-27 105
MS.gene03090.t1 MTR_4g006650 44.828 145 64 3 37 178 5 136 7.86e-25 101
MS.gene03090.t1 MTR_5g012810 34.802 227 137 6 22 238 3 228 4.04e-24 97.8
MS.gene03090.t1 MTR_1g006590 33.906 233 145 6 35 261 18 247 9.65e-24 96.7
MS.gene03090.t1 MTR_4g063090 33.632 223 139 5 41 255 26 247 1.09e-22 94.0
MS.gene03090.t1 MTR_7g103030 32.701 211 134 4 31 234 16 225 1.55e-21 90.9
MS.gene03090.t1 MTR_1g115405 32.068 237 147 6 35 261 20 252 2.22e-20 87.8
MS.gene03090.t1 MTR_2g094270 32.804 189 114 4 59 235 74 261 1.81e-17 80.5
MS.gene03090.t1 MTR_7g101190 29.741 232 142 6 25 235 28 259 1.14e-16 78.2
MS.gene03090.t1 MTR_7g023350 30.472 233 151 4 35 258 20 250 1.67e-16 77.4
MS.gene03090.t1 MTR_5g010150 31.019 216 127 6 39 235 55 267 2.25e-16 77.4
MS.gene03090.t1 MTR_5g082070 31.481 216 126 6 39 235 58 270 2.52e-16 77.4
MS.gene03090.t1 MTR_4g415300 31.604 212 130 6 39 235 59 270 2.59e-16 77.4
MS.gene03090.t1 MTR_3g070210 31.776 214 128 7 39 235 58 270 3.39e-16 77.0
MS.gene03090.t1 MTR_3g118010 29.224 219 140 4 34 237 35 253 4.65e-16 76.6
MS.gene03090.t1 MTR_8g098375 31.308 214 129 7 39 235 55 267 3.01e-15 74.3
MS.gene03090.t1 MTR_2g060360 30.622 209 137 4 35 236 18 225 7.84e-15 72.8
MS.gene03090.t1 MTR_2g094270 31.694 183 114 4 59 232 74 254 4.86e-14 70.5
MS.gene03090.t1 MTR_1g095070 28.571 182 114 4 78 245 24 203 2.94e-13 67.8
MS.gene03090.t1 MTR_3g070210 31.122 196 120 6 39 220 58 252 3.34e-13 68.6
MS.gene03090.t1 MTR_4g059390 30.337 178 112 3 70 235 82 259 5.56e-13 67.8
MS.gene03090.t1 MTR_4g075590 25.339 221 137 7 41 236 42 259 1.82e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03090.t1 AT4G18910 69.880 249 68 1 22 263 38 286 2.88e-126 361
MS.gene03090.t1 AT4G19030 64.591 257 84 1 14 263 33 289 3.65e-121 348
MS.gene03090.t1 AT5G37820 59.426 244 96 1 20 263 30 270 8.18e-100 293
MS.gene03090.t1 AT5G37810 54.962 262 112 1 10 265 11 272 4.90e-99 291
MS.gene03090.t1 AT5G37820 59.426 244 96 1 20 263 66 306 4.98e-99 293
MS.gene03090.t1 AT1G31885 51.852 270 115 3 1 257 4 271 4.26e-95 283
MS.gene03090.t1 AT4G18910 71.351 185 46 1 22 199 38 222 9.96e-89 263
MS.gene03090.t1 AT1G31885 56.682 217 87 1 48 257 1 217 2.88e-86 258
MS.gene03090.t1 AT4G19030 67.568 185 53 1 22 199 41 225 1.45e-85 255
MS.gene03090.t1 AT2G34390 54.812 239 100 2 32 263 44 281 6.09e-85 256
MS.gene03090.t1 AT2G34390 54.545 231 97 2 37 260 7 236 5.26e-83 249
MS.gene03090.t1 AT4G19030 65.455 165 50 1 22 179 41 205 1.40e-73 224
MS.gene03090.t1 AT1G80760 50.204 245 117 2 8 250 52 293 5.84e-72 223
MS.gene03090.t1 AT4G10380 48.617 253 113 4 18 257 56 304 5.13e-69 216
MS.gene03090.t1 AT2G34390 58.286 175 65 2 32 199 44 217 1.04e-63 199
MS.gene03090.t1 AT2G34390 56.688 157 60 2 32 181 44 199 3.53e-55 177
MS.gene03090.t1 AT3G06100 42.202 218 124 2 35 251 45 261 8.39e-48 160
MS.gene03090.t1 AT3G06100 42.925 212 119 2 41 251 3 213 1.91e-47 158
MS.gene03090.t1 AT1G31885 50.000 118 51 2 1 112 4 119 2.00e-33 119
MS.gene03090.t1 AT3G16240 35.945 217 130 4 27 234 8 224 1.12e-30 115
MS.gene03090.t1 AT2G29870 58.621 87 36 0 151 237 20 106 5.72e-30 110
MS.gene03090.t1 AT5G47450 36.123 227 135 5 22 238 3 229 7.44e-29 110
MS.gene03090.t1 AT3G06100 39.437 142 84 2 112 252 41 181 6.58e-26 101
MS.gene03090.t1 AT4G17340 34.434 212 131 4 35 238 18 229 3.46e-25 100
MS.gene03090.t1 AT1G17810 32.271 251 153 6 22 258 13 260 5.73e-25 100
MS.gene03090.t1 AT1G73190 31.200 250 141 6 22 251 13 251 8.31e-24 97.4
MS.gene03090.t1 AT3G26520 33.884 242 139 8 31 258 17 251 2.86e-23 95.9
MS.gene03090.t1 AT2G36830 32.900 231 141 5 13 234 1 226 8.47e-23 94.4
MS.gene03090.t1 AT2G25810 36.493 211 124 7 35 238 18 225 2.81e-22 93.2
MS.gene03090.t1 AT4G01470 33.971 209 129 5 35 235 20 227 6.51e-20 86.7
MS.gene03090.t1 AT4G35100 30.242 248 147 6 16 237 7 254 2.05e-18 83.2
MS.gene03090.t1 AT4G35100 30.242 248 147 6 16 237 7 254 2.05e-18 83.2
MS.gene03090.t1 AT2G16850 30.137 219 138 5 34 237 34 252 6.92e-18 81.6
MS.gene03090.t1 AT5G60660 27.863 262 151 7 33 270 36 283 1.15e-17 81.3
MS.gene03090.t1 AT2G39010 31.771 192 119 3 66 245 77 268 3.97e-17 79.7
MS.gene03090.t1 AT3G47440 29.680 219 138 5 35 242 22 235 5.56e-17 78.6
MS.gene03090.t1 AT3G54820 27.273 253 163 5 14 245 16 268 6.55e-17 79.0
MS.gene03090.t1 AT3G53420 31.383 188 117 4 70 245 82 269 4.57e-16 76.6
MS.gene03090.t1 AT3G53420 31.383 188 117 4 70 245 82 269 4.57e-16 76.6
MS.gene03090.t1 AT1G01620 33.158 190 109 4 61 235 8 194 7.39e-16 74.7
MS.gene03090.t1 AT3G61430 30.986 213 130 6 39 235 55 266 9.29e-16 75.9
MS.gene03090.t1 AT3G61430 30.986 213 130 6 39 235 55 266 9.29e-16 75.9
MS.gene03090.t1 AT1G01620 31.163 215 127 5 39 235 55 266 1.31e-15 75.5
MS.gene03090.t1 AT4G00430 31.163 215 127 5 39 235 56 267 2.88e-15 74.3
MS.gene03090.t1 AT2G37170 29.255 188 121 3 70 245 80 267 5.24e-15 73.6
MS.gene03090.t1 AT4G23400 30.698 215 128 5 39 235 56 267 8.47e-15 73.2
MS.gene03090.t1 AT2G37170 29.255 188 121 3 70 245 123 310 8.81e-15 73.6
MS.gene03090.t1 AT2G45960 30.516 213 131 6 39 235 55 266 1.78e-14 72.0
MS.gene03090.t1 AT2G37180 29.843 191 116 4 70 245 80 267 2.30e-13 68.9
MS.gene03090.t1 AT2G45960 30.769 195 121 5 39 220 55 248 4.16e-12 65.1
MS.gene03090.t1 AT2G45960 30.769 195 121 5 39 220 55 248 5.66e-12 65.1

Find 77 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTTATGTTCGTCATTTC+TGG 0.054064 2.3:+68545327 MS.gene03090:CDS
CTGGCTTATTTGTGATTCTA+AGG 0.227975 2.3:-68545997 None:intergenic
TCCTGGAATTGCAATTGTTT+GGG 0.271099 2.3:+68544494 MS.gene03090:CDS
TTCCTGGAATTGCAATTGTT+TGG 0.289298 2.3:+68544493 MS.gene03090:CDS
ACATTCTTCTTGATATTTGC+TGG 0.306945 2.3:+68544423 MS.gene03090:CDS
TCTTCATTCCTCAAAGGTTT+AGG 0.313855 2.3:+68546040 MS.gene03090:CDS
GTCACCTATTCTTGGAGCTT+TGG 0.322754 2.3:+68545952 MS.gene03090:CDS
GGGACTTGTTGTTATGGTAT+TGG 0.326696 2.3:+68544515 MS.gene03090:CDS
TATTCTATTGGTCATATATC+TGG 0.335738 2.3:+68544540 MS.gene03090:CDS
ATGGTATTGGTCTATTCTAT+TGG 0.341863 2.3:+68544528 MS.gene03090:CDS
GATAAAGTAGTGACACTTCC+TGG 0.350687 2.3:+68544477 MS.gene03090:CDS
CCCCAAACAATTGCAATTCC+AGG 0.359255 2.3:-68544495 None:intergenic
GCAGGCCCTAAGCTTCTTGC+TGG 0.362584 2.3:-68545887 None:intergenic
TTGTAATTTACCTGCTTCAA+TGG 0.368155 2.3:-68544615 None:intergenic
TCCATATTCCTCTATATTGA+TGG 0.376585 2.3:-68545919 None:intergenic
ATTGCTTCAGATTGGTGAAT+TGG 0.377943 2.3:+68545636 MS.gene03090:intron
TCAATTTGCAGGAACACTTC+CGG 0.378252 2.3:+68545257 MS.gene03090:CDS
CCATGCTTCCTAAACCTTTG+AGG 0.382124 2.3:-68546048 None:intergenic
ATGCTAAATGTGTTGTTTGC+GGG 0.383890 2.3:+68545685 MS.gene03090:CDS
TCACTACTTTATCATTGTTA+AGG 0.387999 2.3:-68544467 None:intergenic
GGCAAGGAAAATCAATTTGC+AGG 0.389055 2.3:+68545246 MS.gene03090:CDS
TATATGGTGTCACCTATTCT+TGG 0.391277 2.3:+68545944 MS.gene03090:CDS
CCTATTCTTGGAGCTTTGGC+TGG 0.403085 2.3:+68545956 MS.gene03090:CDS
TAGAGGAATATGGATATATA+TGG 0.403479 2.3:+68545928 MS.gene03090:CDS
AGGAACACTTCCGGCTGGTT+CGG 0.404188 2.3:+68545266 MS.gene03090:CDS
CTTCAGATTGGTGAATTGGC+TGG 0.406978 2.3:+68545640 MS.gene03090:intron
TTGGAGCTTTGGCTGGTACA+TGG 0.409617 2.3:+68545963 MS.gene03090:CDS
CTGCTTCAATGGAAACCTTC+CGG 0.415943 2.3:-68544604 None:intergenic
TGTTTGGGGACTTGTTGTTA+TGG 0.418172 2.3:+68544509 MS.gene03090:CDS
ATGAATCCAGCAAGAAGCTT+AGG 0.418884 2.3:+68545881 MS.gene03090:CDS
TTCTTGATATTTGCTGGATG+TGG 0.422703 2.3:+68544429 MS.gene03090:CDS
ATTCGACCACAAAAGCTTGA+TGG 0.429541 2.3:-68545290 None:intergenic
TGGAGCTTTGGCTGGTACAT+GGG 0.431169 2.3:+68545964 MS.gene03090:CDS
ACCATCAATATAGAGGAATA+TGG 0.435935 2.3:+68545918 MS.gene03090:CDS
GCTCTCAAACTCATATTTAG+TGG 0.443354 2.3:+68545225 MS.gene03090:CDS
TTTGAGGAATGAAGAACTCT+TGG 0.447025 2.3:-68546032 None:intergenic
CCAGCCAAAGCTCCAAGAAT+AGG 0.450629 2.3:-68545956 None:intergenic
CTCCAGAAATGACGAACATA+AGG 0.455985 2.3:-68545329 None:intergenic
TATATAATTGCTCAGGTCGC+TGG 0.462381 2.3:+68545183 MS.gene03090:CDS
ATTCATTGATGCTCCTGTGA+TGG 0.469127 2.3:-68545864 None:intergenic
TTCATTGATGCTCCTGTGAT+GGG 0.472526 2.3:-68545863 None:intergenic
CCTCAAAGGTTTAGGAAGCA+TGG 0.503782 2.3:+68546048 MS.gene03090:CDS
CGGAAGGTTTCCATTGAAGC+AGG 0.504912 2.3:+68544605 MS.gene03090:CDS
TATTGATGGTGCACAATAGC+AGG 0.505141 2.3:-68545905 None:intergenic
TGAATCCAGCAAGAAGCTTA+GGG 0.507995 2.3:+68545882 MS.gene03090:CDS
CCTGGAATTGCAATTGTTTG+GGG 0.511430 2.3:+68544495 MS.gene03090:CDS
GATTGTTGAATATAGTTGGT+AGG 0.511991 2.3:+68544387 MS.gene03090:intron
CATGCTAAATGTGTTGTTTG+CGG 0.516473 2.3:+68545684 MS.gene03090:CDS
GAATTGGCTGGACTTGCAGT+TGG 0.518324 2.3:+68545652 MS.gene03090:CDS
CTCAAAGGTTTAGGAAGCAT+GGG 0.520761 2.3:+68546049 MS.gene03090:CDS
CTCTTGGTGAGCTCACGTAC+TGG 0.527633 2.3:-68546016 None:intergenic
ACCCGCTTATATAATTGCTC+AGG 0.527901 2.3:+68545176 MS.gene03090:CDS
AAGAGGAACATGATCATCAA+TGG 0.530972 2.3:-68544275 None:intergenic
ACCTGAGCAATTATATAAGC+GGG 0.540434 2.3:-68545177 None:intergenic
GTGTGAGCAATGGTGACAGC+GGG 0.558512 2.3:-68544576 None:intergenic
AACATGGGTGATATTAGCAA+TGG 0.558983 2.3:+68544193 None:intergenic
ACCATTGCTCACACTACCAC+CGG 0.562160 2.3:+68544585 MS.gene03090:CDS
TGCTGGATGTGGTTCTGTGG+TGG 0.564205 2.3:+68544440 MS.gene03090:CDS
AAGAGTTCTTCATTCCTCAA+AGG 0.566116 2.3:+68546034 MS.gene03090:CDS
GCTTGATGGTCCGAACCAGC+CGG 0.573042 2.3:-68545276 None:intergenic
TGCTAAATGTGTTGTTTGCG+GGG 0.584865 2.3:+68545686 MS.gene03090:CDS
TTTGCAGGAACACTTCCGGC+TGG 0.585819 2.3:+68545261 MS.gene03090:CDS
ATAGTTGGTAGGAGAAGTTG+TGG 0.585849 2.3:+68544398 MS.gene03090:intron
CAAACTCATATTTAGTGGCA+AGG 0.586123 2.3:+68545230 MS.gene03090:CDS
ATTGTGCACCATCAATATAG+AGG 0.592044 2.3:+68545911 MS.gene03090:CDS
ATTTGCTGGATGTGGTTCTG+TGG 0.593346 2.3:+68544437 MS.gene03090:CDS
AATTACCGCTCTATTATCAG+TGG 0.623253 2.3:-68545355 None:intergenic
ATATTTGACAGACCCATCAC+AGG 0.629986 2.3:+68545851 MS.gene03090:intron
GACCTGAGCAATTATATAAG+CGG 0.630532 2.3:-68545178 None:intergenic
TAGCAATGGAAATCTTGATG+TGG 0.656350 2.3:+68544207 MS.gene03090:CDS
TTGCTCACACTACCACCGGA+AGG 0.656735 2.3:+68544589 MS.gene03090:CDS
TCCGGTGGTAGTGTGAGCAA+TGG 0.668876 2.3:-68544586 None:intergenic
TAGTTGGTAGGAGAAGTTGT+GGG 0.670961 2.3:+68544399 MS.gene03090:intron
CTTCAATGGAAACCTTCCGG+TGG 0.695539 2.3:-68544601 None:intergenic
AGTGTGAGCAATGGTGACAG+CGG 0.739883 2.3:-68544577 None:intergenic
AGTTGCCACTGATAATAGAG+CGG 0.742875 2.3:+68545350 MS.gene03090:CDS
GTGGTTATGAATGTAAACGA+TGG 0.756470 2.3:+68544226 MS.gene03090:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAAAATTAATAATATTT+TGG - chr2.3:68545138-68545157 None:intergenic 0.0%
!! TAGATAAAAATGTATAATAT+TGG - chr2.3:68545764-68545783 None:intergenic 10.0%
!!! ATTATACATTTTTATCTAAT+AGG + chr2.3:68545766-68545785 MS.gene03090:intron 10.0%
!!! ATTTTTTTTTTTTAAATGCT+TGG + chr2.3:68545375-68545394 MS.gene03090:intron 10.0%
!!! TTATACATTTTTATCTAATA+GGG + chr2.3:68545767-68545786 MS.gene03090:intron 10.0%
!!! TTTATTTAATTTTTTCTTGT+AGG + chr2.3:68545152-68545171 MS.gene03090:intron 10.0%
!! AAATGAATAATATTAGACAA+AGG - chr2.3:68545611-68545630 None:intergenic 15.0%
!! TTATGATATAATATTTGGTA+AGG - chr2.3:68545726-68545745 None:intergenic 15.0%
!!! GCATTTTATGATATAATATT+TGG - chr2.3:68545731-68545750 None:intergenic 15.0%
!!! TATTGATTGTTGAATATAGT+TGG + chr2.3:68544383-68544402 MS.gene03090:intron 20.0%
!!! TTTTATTTATTGCTTCAGAT+TGG + chr2.3:68545628-68545647 MS.gene03090:intron 20.0%
! AGATCTTCTGACTAAAAAAA+TGG + chr2.3:68544359-68544378 MS.gene03090:intron 25.0%
! TAGAGGAATATGGATATATA+TGG + chr2.3:68545928-68545947 MS.gene03090:CDS 25.0%
! TATTCTATTGGTCATATATC+TGG + chr2.3:68544540-68544559 MS.gene03090:CDS 25.0%
! TCACTACTTTATCATTGTTA+AGG - chr2.3:68544470-68544489 None:intergenic 25.0%
! TCTCTCTTAAGTTATTTGAT+CGG + chr2.3:68544678-68544697 MS.gene03090:intron 25.0%
!!! AATATTTTGGACTGCAAAAA+TGG - chr2.3:68545125-68545144 None:intergenic 25.0%
!!! AGCTAAGAGTTTAAAGTTTT+GGG + chr2.3:68544795-68544814 MS.gene03090:intron 25.0%
ACATTCTTCTTGATATTTGC+TGG + chr2.3:68544423-68544442 MS.gene03090:CDS 30.0%
ACCATCAATATAGAGGAATA+TGG + chr2.3:68545918-68545937 MS.gene03090:CDS 30.0%
GATTGTTGAATATAGTTGGT+AGG + chr2.3:68544387-68544406 MS.gene03090:intron 30.0%
TAAATGCTTAGAGTAACAGT+TGG + chr2.3:68544861-68544880 MS.gene03090:intron 30.0%
TATTTCCTTCTTCTTCACAT+AGG - chr2.3:68545049-68545068 None:intergenic 30.0%
TCCATATTCCTCTATATTGA+TGG - chr2.3:68545922-68545941 None:intergenic 30.0%
TTAAGTTATTTGATCGGAGA+TGG + chr2.3:68544684-68544703 MS.gene03090:intron 30.0%
TTGTAATTTACCTGCTTCAA+TGG - chr2.3:68544618-68544637 None:intergenic 30.0%
! AAAATCACCTTTTGCAAAAG+AGG - chr2.3:68544295-68544314 None:intergenic 30.0%
!! ATGGTATTGGTCTATTCTAT+TGG + chr2.3:68544528-68544547 MS.gene03090:CDS 30.0%
!! GAGCTAAGAGTTTAAAGTTT+TGG + chr2.3:68544794-68544813 MS.gene03090:intron 30.0%
!! GCTAAGAGTTTAAAGTTTTG+GGG + chr2.3:68544796-68544815 MS.gene03090:intron 30.0%
!!! GTTACATGTTTTAGTGAGAA+AGG + chr2.3:68545797-68545816 MS.gene03090:intron 30.0%
AAGAGGAACATGATCATCAA+TGG - chr2.3:68544278-68544297 None:intergenic 35.0%
AAGAGTTCTTCATTCCTCAA+AGG + chr2.3:68546034-68546053 MS.gene03090:CDS 35.0%
AATTACCGCTCTATTATCAG+TGG - chr2.3:68545358-68545377 None:intergenic 35.0%
ACCTGAGCAATTATATAAGC+GGG - chr2.3:68545180-68545199 None:intergenic 35.0%
AGAAGAAGGAAATAGTTGGT+AGG + chr2.3:68545055-68545074 MS.gene03090:intron 35.0%
ATAATTATAGAATGGGCCTC+AGG - chr2.3:68545573-68545592 None:intergenic 35.0%
ATTGCTTCAGATTGGTGAAT+TGG + chr2.3:68545636-68545655 MS.gene03090:intron 35.0%
ATTGTGCACCATCAATATAG+AGG + chr2.3:68545911-68545930 MS.gene03090:CDS 35.0%
CAAACTCATATTTAGTGGCA+AGG + chr2.3:68545230-68545249 MS.gene03090:CDS 35.0%
CCAACTAATGCTTCAAACAT+AGG - chr2.3:68545093-68545112 None:intergenic 35.0%
CTGGCTTATTTGTGATTCTA+AGG - chr2.3:68546000-68546019 None:intergenic 35.0%
GACCTGAGCAATTATATAAG+CGG - chr2.3:68545181-68545200 None:intergenic 35.0%
GCTCGATGTAGTAACAAAAA+GGG + chr2.3:68544989-68545008 MS.gene03090:intron 35.0%
GCTCTCAAACTCATATTTAG+TGG + chr2.3:68545225-68545244 MS.gene03090:CDS 35.0%
GTGAAGAAGAAGGAAATAGT+TGG + chr2.3:68545051-68545070 MS.gene03090:intron 35.0%
GTGGTTATGAATGTAAACGA+TGG + chr2.3:68544226-68544245 MS.gene03090:CDS 35.0%
TACCTTATGTTCGTCATTTC+TGG + chr2.3:68545327-68545346 MS.gene03090:CDS 35.0%
TAGCAATGGAAATCTTGATG+TGG + chr2.3:68544207-68544226 MS.gene03090:CDS 35.0%
TCAAACATAGGCCCAAATAA+CGG - chr2.3:68545081-68545100 None:intergenic 35.0%
TCCTGGAATTGCAATTGTTT+GGG + chr2.3:68544494-68544513 MS.gene03090:CDS 35.0%
TCTTCATTCCTCAAAGGTTT+AGG + chr2.3:68546040-68546059 MS.gene03090:CDS 35.0%
TGCTCGATGTAGTAACAAAA+AGG + chr2.3:68544988-68545007 MS.gene03090:intron 35.0%
TTACCTTTAAATCAGTCCTG+AGG + chr2.3:68545554-68545573 MS.gene03090:intron 35.0%
TTCAACCTATGTGAAGAAGA+AGG + chr2.3:68545041-68545060 MS.gene03090:intron 35.0%
TTCCTGGAATTGCAATTGTT+TGG + chr2.3:68544493-68544512 MS.gene03090:CDS 35.0%
TTTGAGGAATGAAGAACTCT+TGG - chr2.3:68546035-68546054 None:intergenic 35.0%
! AATAAAGGCGCAATGTTACA+AGG - chr2.3:68544927-68544946 None:intergenic 35.0%
! ACATAGGTTGAAACTTGCTT+TGG - chr2.3:68545033-68545052 None:intergenic 35.0%
! CCTATGTTTGAAGCATTAGT+TGG + chr2.3:68545090-68545109 MS.gene03090:intron 35.0%
! TCATGTTCCTCTTTTGCAAA+AGG + chr2.3:68544285-68544304 MS.gene03090:CDS 35.0%
! TTCTTGATATTTGCTGGATG+TGG + chr2.3:68544429-68544448 MS.gene03090:CDS 35.0%
!! ATGCTAAATGTGTTGTTTGC+GGG + chr2.3:68545685-68545704 MS.gene03090:CDS 35.0%
!! CATGCTAAATGTGTTGTTTG+CGG + chr2.3:68545684-68545703 MS.gene03090:CDS 35.0%
!! TATATGGTGTCACCTATTCT+TGG + chr2.3:68545944-68545963 MS.gene03090:CDS 35.0%
AAAGGGTGGTGTGAAAAGTA+TGG + chr2.3:68545006-68545025 MS.gene03090:intron 40.0%
ACCCGCTTATATAATTGCTC+AGG + chr2.3:68545176-68545195 MS.gene03090:CDS 40.0%
AGTTGCCACTGATAATAGAG+CGG + chr2.3:68545350-68545369 MS.gene03090:CDS 40.0%
ATATTTGACAGACCCATCAC+AGG + chr2.3:68545851-68545870 MS.gene03090:intron 40.0%
ATGAATCCAGCAAGAAGCTT+AGG + chr2.3:68545881-68545900 MS.gene03090:CDS 40.0%
ATTCATTGATGCTCCTGTGA+TGG - chr2.3:68545867-68545886 None:intergenic 40.0%
ATTCGACCACAAAAGCTTGA+TGG - chr2.3:68545293-68545312 None:intergenic 40.0%
CCTGGAATTGCAATTGTTTG+GGG + chr2.3:68544495-68544514 MS.gene03090:CDS 40.0%
CGATGTAGTAACAAAAAGGG+TGG + chr2.3:68544992-68545011 MS.gene03090:intron 40.0%
CTCCAGAAATGACGAACATA+AGG - chr2.3:68545332-68545351 None:intergenic 40.0%
GAGATTGTAGGTGGTTGAAA+AGG + chr2.3:68544727-68544746 MS.gene03090:intron 40.0%
GATAAAGTAGTGACACTTCC+TGG + chr2.3:68544477-68544496 MS.gene03090:CDS 40.0%
GGCAAGGAAAATCAATTTGC+AGG + chr2.3:68545246-68545265 MS.gene03090:CDS 40.0%
TATATAATTGCTCAGGTCGC+TGG + chr2.3:68545183-68545202 MS.gene03090:CDS 40.0%
TCAATTTGCAGGAACACTTC+CGG + chr2.3:68545257-68545276 MS.gene03090:CDS 40.0%
TGAATCCAGCAAGAAGCTTA+GGG + chr2.3:68545882-68545901 MS.gene03090:CDS 40.0%
TTCATTGATGCTCCTGTGAT+GGG - chr2.3:68545866-68545885 None:intergenic 40.0%
! CTCAAAGGTTTAGGAAGCAT+GGG + chr2.3:68546049-68546068 MS.gene03090:CDS 40.0%
!! ATAGTTGGTAGGAGAAGTTG+TGG + chr2.3:68544398-68544417 MS.gene03090:intron 40.0%
!! ATAGTTGGTAGGCCGTTATT+TGG + chr2.3:68545066-68545085 MS.gene03090:intron 40.0%
!! GGGACTTGTTGTTATGGTAT+TGG + chr2.3:68544515-68544534 MS.gene03090:CDS 40.0%
!! TAGTTGGTAGGAGAAGTTGT+GGG + chr2.3:68544399-68544418 MS.gene03090:intron 40.0%
!! TAGTTGGTAGGCCGTTATTT+GGG + chr2.3:68545067-68545086 MS.gene03090:intron 40.0%
!! TATTGATGGTGCACAATAGC+AGG - chr2.3:68545908-68545927 None:intergenic 40.0%
!! TGCTAAATGTGTTGTTTGCG+GGG + chr2.3:68545686-68545705 MS.gene03090:CDS 40.0%
!! TGTTTGGGGACTTGTTGTTA+TGG + chr2.3:68544509-68544528 MS.gene03090:CDS 40.0%
AAGAACGTACCTAGGAGGAT+AGG + chr2.3:68544823-68544842 MS.gene03090:intron 45.0%
ATCGGAGATGGTCAGAAGAA+CGG + chr2.3:68544696-68544715 MS.gene03090:intron 45.0%
CCATGCTTCCTAAACCTTTG+AGG - chr2.3:68546051-68546070 None:intergenic 45.0%
CCCCAAACAATTGCAATTCC+AGG - chr2.3:68544498-68544517 None:intergenic 45.0%
CCTCAAAGGTTTAGGAAGCA+TGG + chr2.3:68546048-68546067 MS.gene03090:CDS 45.0%
CTGCTTCAATGGAAACCTTC+CGG - chr2.3:68544607-68544626 None:intergenic 45.0%
CTTCAGATTGGTGAATTGGC+TGG + chr2.3:68545640-68545659 MS.gene03090:intron 45.0%
GGTACCTGCAAAAGACACAT+TGG + chr2.3:68544754-68544773 MS.gene03090:intron 45.0%
GTCACCTATTCTTGGAGCTT+TGG + chr2.3:68545952-68545971 MS.gene03090:CDS 45.0%
GTTAGAAGAACGTACCTAGG+AGG + chr2.3:68544818-68544837 MS.gene03090:intron 45.0%
TCAACCACCTACAATCTCGT+CGG - chr2.3:68544725-68544744 None:intergenic 45.0%
! AACGCCAATGTGTCTTTTGC+AGG - chr2.3:68544761-68544780 None:intergenic 45.0%
! ATTTGCTGGATGTGGTTCTG+TGG + chr2.3:68544437-68544456 MS.gene03090:CDS 45.0%
! GTTTGAAGCATTAGTTGGCG+AGG + chr2.3:68545095-68545114 MS.gene03090:intron 45.0%
! TTCGGACCATCAAGCTTTTG+TGG + chr2.3:68545284-68545303 MS.gene03090:CDS 45.0%
!! AGTAACAGTTGGTGAGCTGT+TGG + chr2.3:68544872-68544891 MS.gene03090:intron 45.0%
!! ATATTTAAATAATTATAGAA+TGG - chr2.3:68545581-68545600 None:intergenic 5.0%
!! TATTTAAATAATTATAGAAT+GGG - chr2.3:68545580-68545599 None:intergenic 5.0%
ACCATTGCTCACACTACCAC+CGG + chr2.3:68544585-68544604 MS.gene03090:CDS 50.0%
ACGGTATCCGACGAGATTGT+AGG + chr2.3:68544715-68544734 MS.gene03090:intron 50.0%
AGAGCTTCTCCTATCCTCCT+AGG - chr2.3:68544835-68544854 None:intergenic 50.0%
AGTGTGAGCAATGGTGACAG+CGG - chr2.3:68544580-68544599 None:intergenic 50.0%
CCAGCCAAAGCTCCAAGAAT+AGG - chr2.3:68545959-68545978 None:intergenic 50.0%
CGGAAGGTTTCCATTGAAGC+AGG + chr2.3:68544605-68544624 MS.gene03090:CDS 50.0%
CTTCAATGGAAACCTTCCGG+TGG - chr2.3:68544604-68544623 None:intergenic 50.0%
GAATTGGCTGGACTTGCAGT+TGG + chr2.3:68545652-68545671 MS.gene03090:CDS 50.0%
GGGGTTAGAAGAACGTACCT+AGG + chr2.3:68544815-68544834 MS.gene03090:intron 50.0%
GTAGGTGGTTGAAAAGGCAG+AGG + chr2.3:68544733-68544752 MS.gene03090:intron 50.0%
GTATCCGACGAGATTGTAGG+TGG + chr2.3:68544718-68544737 MS.gene03090:intron 50.0%
TGTTGGTCGACGGTTACAGA+TGG + chr2.3:68544889-68544908 MS.gene03090:intron 50.0%
TTGCGCCTTTATTGCACGAG+AGG + chr2.3:68544934-68544953 MS.gene03090:intron 50.0%
! CCTATTCTTGGAGCTTTGGC+TGG + chr2.3:68545956-68545975 MS.gene03090:CDS 50.0%
! GGGCCTCAGGACTGATTTAA+AGG - chr2.3:68545560-68545579 None:intergenic 50.0%
! TGGAGCTTTGGCTGGTACAT+GGG + chr2.3:68545964-68545983 MS.gene03090:CDS 50.0%
! TTGGAGCTTTGGCTGGTACA+TGG + chr2.3:68545963-68545982 MS.gene03090:CDS 50.0%
CGCCTCCTCTCGTGCAATAA+AGG - chr2.3:68544942-68544961 None:intergenic 55.0%
CGCCTTTATTGCACGAGAGG+AGG + chr2.3:68544937-68544956 MS.gene03090:intron 55.0%
CTCTTGGTGAGCTCACGTAC+TGG - chr2.3:68546019-68546038 None:intergenic 55.0%
GTGTGAGCAATGGTGACAGC+GGG - chr2.3:68544579-68544598 None:intergenic 55.0%
TGGTCGACGGTTACAGATGG+CGG + chr2.3:68544892-68544911 MS.gene03090:intron 55.0%
TTGCTCACACTACCACCGGA+AGG + chr2.3:68544589-68544608 MS.gene03090:CDS 55.0%
TTTGCAGGAACACTTCCGGC+TGG + chr2.3:68545261-68545280 MS.gene03090:CDS 55.0%
! AGGAACACTTCCGGCTGGTT+CGG + chr2.3:68545266-68545285 MS.gene03090:CDS 55.0%
! GTTGGTGAGCTGTTGGTCGA+CGG + chr2.3:68544879-68544898 MS.gene03090:intron 55.0%
! TCCGGTGGTAGTGTGAGCAA+TGG - chr2.3:68544589-68544608 None:intergenic 55.0%
! TGCTGGATGTGGTTCTGTGG+TGG + chr2.3:68544440-68544459 MS.gene03090:CDS 55.0%
!! AGAGGAGGCGTTACAGACTC+AGG + chr2.3:68544952-68544971 MS.gene03090:intron 55.0%
GCAGGCCCTAAGCTTCTTGC+TGG - chr2.3:68545890-68545909 None:intergenic 60.0%
! GCTTGATGGTCCGAACCAGC+CGG - chr2.3:68545279-68545298 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 68544196 68546081 68544196 ID=MS.gene03090
chr2.3 mRNA 68544196 68546081 68544196 ID=MS.gene03090.t1;Parent=MS.gene03090
chr2.3 exon 68544196 68544306 68544196 ID=MS.gene03090.t1.exon1;Parent=MS.gene03090.t1
chr2.3 CDS 68544196 68544306 68544196 ID=cds.MS.gene03090.t1;Parent=MS.gene03090.t1
chr2.3 exon 68544402 68544626 68544402 ID=MS.gene03090.t1.exon2;Parent=MS.gene03090.t1
chr2.3 CDS 68544402 68544626 68544402 ID=cds.MS.gene03090.t1;Parent=MS.gene03090.t1
chr2.3 exon 68545174 68545371 68545174 ID=MS.gene03090.t1.exon3;Parent=MS.gene03090.t1
chr2.3 CDS 68545174 68545371 68545174 ID=cds.MS.gene03090.t1;Parent=MS.gene03090.t1
chr2.3 exon 68545646 68545707 68545646 ID=MS.gene03090.t1.exon4;Parent=MS.gene03090.t1
chr2.3 CDS 68545646 68545707 68545646 ID=cds.MS.gene03090.t1;Parent=MS.gene03090.t1
chr2.3 exon 68545862 68546081 68545862 ID=MS.gene03090.t1.exon5;Parent=MS.gene03090.t1
chr2.3 CDS 68545862 68546081 68545862 ID=cds.MS.gene03090.t1;Parent=MS.gene03090.t1
Gene Sequence

>MS.gene03090

ATGGGTGATATTAGCAATGGAAATCTTGATGTGGTTATGAATGTAAACGATGGTGCAACAAAAAAATGTGACGACACAACCATTGATGATCATGTTCCTCTTTTGCAAAAGGTGATTTTCTTTTTCATTCAAAAAATTAATTGTTGTTTATGATTAATTAATTAGATCTTCTGACTAAAAAAATGGATATTGATTGTTGAATATAGTTGGTAGGAGAAGTTGTGGGAACATTCTTCTTGATATTTGCTGGATGTGGTTCTGTGGTGGTGAACCTTAACAATGATAAAGTAGTGACACTTCCTGGAATTGCAATTGTTTGGGGACTTGTTGTTATGGTATTGGTCTATTCTATTGGTCATATATCTGGTGCTCATTTCAATCCCGCTGTCACCATTGCTCACACTACCACCGGAAGGTTTCCATTGAAGCAGGTAAATTACAAAACACAAGAACAATAATTCATATTAAGATAAACATAATTTTCTCTCTTAAGTTATTTGATCGGAGATGGTCAGAAGAACGGTATCCGACGAGATTGTAGGTGGTTGAAAAGGCAGAGGTACCTGCAAAAGACACATTGGCGTTCAAGTCAGCATGAGAGCTAAGAGTTTAAAGTTTTGGGGTTAGAAGAACGTACCTAGGAGGATAGGAGAAGCTCTCTTATATAAATGCTTAGAGTAACAGTTGGTGAGCTGTTGGTCGACGGTTACAGATGGCGGTGTTTGTAACCTTGTAACATTGCGCCTTTATTGCACGAGAGGAGGCGTTACAGACTCAGGTGAGATGAACGTTTGCTCGATGTAGTAACAAAAAGGGTGGTGTGAAAAGTATGGACCAAAGCAAGTTTCAACCTATGTGAAGAAGAAGGAAATAGTTGGTAGGCCGTTATTTGGGCCTATGTTTGAAGCATTAGTTGGCGAGGACAACCATTTTTGCAGTCCAAAATATTATTAATTTTTATTTAATTTTTTCTTGTAGGTACCCGCTTATATAATTGCTCAGGTCGCTGGCTCAACACTTGCAAGTGAAGCTCTCAAACTCATATTTAGTGGCAAGGAAAATCAATTTGCAGGAACACTTCCGGCTGGTTCGGACCATCAAGCTTTTGTGGTCGAATTTATTATCACTTTCTACCTTATGTTCGTCATTTCTGGAGTTGCCACTGATAATAGAGCGGTAATTTTTTTTTTTTAAATGCTTGGTTGATAATAGTAATTGATAATTATATTTCTATTGATCATTTTGATTAAGATAAAATTGTGACTCAAGTTAATTGACTATTTCTTTCAACAACAATTAAAAGCTCATTGACTAGATGTATAAACATATTTATAATGTTAAAATCACTTCAATAAATGTTACCTTTAAATCAGTCCTGAGGCCCATTCTATAATTATTTAAATATCATTTAACCTTTGTCTAATATTATTCATTTTATTTATTGCTTCAGATTGGTGAATTGGCTGGACTTGCAGTTGGATCTACAGTCATGCTAAATGTGTTGTTTGCGGGGTATAGATCTCAAAACCTTACCAAATATTATATCATAAAATGCATGCTTTGCACCAATATTATACATTTTTATCTAATAGGGTTAATATGTTACATGTTTTAGTGAGAAAGGTTCAAATTAAATCTTACTTTTGAGTGAAAATATATTTGACAGACCCATCACAGGAGCATCAATGAATCCAGCAAGAAGCTTAGGGCCTGCTATTGTGCACCATCAATATAGAGGAATATGGATATATATGGTGTCACCTATTCTTGGAGCTTTGGCTGGTACATGGGCATACACTTTCCTTAGAATCACAAATAAGCCAGTACGTGAGCTCACCAAGAGTTCTTCATTCCTCAAAGGTTTAGGAAGCATGGGAGCTGAGTGA
Protein sequence

>MS.gene03090.t1

MGDISNGNLDVVMNVNDGATKKCDDTTIDDHVPLLQKLVGEVVGTFFLIFAGCGSVVVNLNNDKVVTLPGIAIVWGLVVMVLVYSIGHISGAHFNPAVTIAHTTTGRFPLKQVPAYIIAQVAGSTLASEALKLIFSGKENQFAGTLPAGSDHQAFVVEFIITFYLMFVISGVATDNRAIGELAGLAVGSTVMLNVLFAGPITGASMNPARSLGPAIVHHQYRGIWIYMVSPILGALAGTWAYTFLRITNKPVRELTKSSSFLKGLGSMGAE