AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene002639

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002639.t1 MTR_2g017570 97.802 273 6 0 1 273 1 273 0.0 547
MS.gene002639.t1 MTR_2g017610 97.070 273 8 0 1 273 1 273 0.0 541
MS.gene002639.t1 MTR_2g017590 81.061 264 50 0 7 270 5 268 5.24e-163 453
MS.gene002639.t1 MTR_2g017620 78.195 266 58 0 1 266 1 266 3.28e-154 431
MS.gene002639.t1 MTR_8g087720 76.493 268 63 0 1 268 1 268 1.02e-152 427
MS.gene002639.t1 MTR_2g017620 79.365 252 52 0 15 266 1 252 2.19e-148 416
MS.gene002639.t1 MTR_8g087710 70.480 271 80 0 3 273 4 274 2.70e-142 401
MS.gene002639.t1 MTR_2g017660 69.853 272 75 2 1 265 1 272 3.39e-141 398
MS.gene002639.t1 MTR_2g017620 82.703 185 32 0 82 266 1 185 7.93e-111 318
MS.gene002639.t1 MTR_8g087720 77.612 201 45 0 1 201 1 201 8.87e-111 318
MS.gene002639.t1 MTR_4g084880 58.025 243 100 1 32 272 29 271 1.62e-102 300
MS.gene002639.t1 MTR_8g087720 74.457 184 47 0 1 184 1 184 3.83e-96 281
MS.gene002639.t1 MTR_5g079570 49.612 258 121 3 1 253 1 254 1.01e-84 254
MS.gene002639.t1 MTR_3g008920 53.556 239 108 2 18 256 8 243 6.43e-84 252
MS.gene002639.t1 MTR_4g006730 50.840 238 108 3 34 265 71 305 2.49e-70 219
MS.gene002639.t1 MTR_7g010650 47.085 223 115 2 31 251 28 249 8.49e-69 214
MS.gene002639.t1 MTR_2g104940 51.163 215 100 2 36 248 78 289 1.63e-67 212
MS.gene002639.t1 MTR_5g063930 45.522 268 129 7 1 260 1 259 6.05e-67 209
MS.gene002639.t1 MTR_1g097840 46.800 250 114 4 26 259 54 300 3.26e-66 208
MS.gene002639.t1 MTR_4g006630 50.226 221 101 3 51 265 1 218 4.30e-64 200
MS.gene002639.t1 MTR_4g006730 50.226 221 101 3 51 265 1 218 6.57e-64 199
MS.gene002639.t1 MTR_4g006730 52.941 170 75 2 34 201 71 237 5.27e-47 158
MS.gene002639.t1 MTR_1g070385 39.906 213 127 1 40 252 64 275 2.07e-44 160
MS.gene002639.t1 MTR_1g097840 47.849 186 84 3 26 201 54 236 1.39e-43 149
MS.gene002639.t1 MTR_7g010650 43.137 153 84 2 31 181 28 179 1.71e-37 131
MS.gene002639.t1 MTR_4g006650 47.222 144 62 3 39 180 5 136 7.14e-27 107
MS.gene002639.t1 MTR_8g013680 32.850 207 132 3 37 236 18 224 1.52e-25 101
MS.gene002639.t1 MTR_1g077540 32.218 239 144 6 26 253 12 243 5.52e-24 97.8
MS.gene002639.t1 MTR_2g101370 30.568 229 150 5 37 256 19 247 9.47e-23 94.4
MS.gene002639.t1 MTR_5g012810 33.913 230 141 7 37 256 18 246 1.56e-22 93.6
MS.gene002639.t1 MTR_1g006490 32.143 224 136 7 19 236 7 220 5.42e-22 92.0
MS.gene002639.t1 MTR_6g069040 29.583 240 153 6 24 253 10 243 7.96e-22 92.0
MS.gene002639.t1 MTR_1g006590 32.000 250 150 9 19 258 7 246 3.70e-21 90.1
MS.gene002639.t1 MTR_4g063090 32.159 227 140 7 43 256 26 251 2.69e-17 79.3
MS.gene002639.t1 MTR_7g103030 30.667 225 146 6 37 252 20 243 3.83e-17 79.0
MS.gene002639.t1 MTR_3g118010 28.384 229 141 5 38 247 37 261 1.72e-16 77.8
MS.gene002639.t1 MTR_2g094270 30.899 178 111 3 72 237 84 261 2.09e-16 77.8
MS.gene002639.t1 MTR_5g082070 31.019 216 127 7 41 237 58 270 3.79e-16 77.0
MS.gene002639.t1 MTR_7g101190 29.412 221 135 6 38 237 39 259 1.28e-15 75.5
MS.gene002639.t1 MTR_5g010150 30.047 213 133 5 41 237 55 267 1.36e-15 75.5
MS.gene002639.t1 MTR_1g115405 32.227 211 130 5 37 237 20 227 1.61e-15 74.7
MS.gene002639.t1 MTR_8g098375 30.516 213 132 6 41 237 55 267 1.74e-15 75.1
MS.gene002639.t1 MTR_3g070210 28.638 213 136 5 41 237 58 270 1.14e-14 72.8
MS.gene002639.t1 MTR_4g415300 29.245 212 135 5 41 237 59 270 2.24e-14 72.0
MS.gene002639.t1 MTR_2g094270 30.573 157 100 2 72 219 84 240 7.05e-14 70.1
MS.gene002639.t1 MTR_3g070210 28.205 195 127 4 41 222 58 252 2.62e-13 68.6
MS.gene002639.t1 MTR_1g095070 28.571 182 112 4 81 247 25 203 3.11e-13 67.8
MS.gene002639.t1 MTR_2g060360 26.639 244 164 5 37 273 18 253 9.36e-13 67.0
MS.gene002639.t1 MTR_7g023350 30.263 228 135 8 37 251 20 236 9.43e-13 66.6
MS.gene002639.t1 MTR_4g059390 29.282 181 110 5 72 237 82 259 7.72e-12 64.7
MS.gene002639.t1 MTR_1g095070 28.571 154 95 3 81 222 25 175 4.15e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002639.t1 AT4G18910 70.980 255 67 1 26 273 40 294 3.60e-131 373
MS.gene002639.t1 AT4G19030 68.651 252 72 1 26 270 43 294 4.31e-125 358
MS.gene002639.t1 AT5G37820 58.431 255 103 1 18 269 20 274 7.56e-100 293
MS.gene002639.t1 AT5G37820 58.431 255 103 1 18 269 56 310 2.88e-99 293
MS.gene002639.t1 AT5G37810 54.015 274 119 3 1 268 1 273 1.36e-98 290
MS.gene002639.t1 AT4G18910 75.410 183 38 1 26 201 40 222 5.79e-92 271
MS.gene002639.t1 AT1G31885 53.036 247 109 1 20 259 25 271 2.96e-90 270
MS.gene002639.t1 AT2G34390 51.748 286 120 3 1 269 1 285 3.12e-90 269
MS.gene002639.t1 AT4G19030 70.330 182 47 1 26 200 43 224 1.74e-87 260
MS.gene002639.t1 AT2G34390 55.462 238 98 2 39 269 7 243 4.78e-86 257
MS.gene002639.t1 AT1G31885 54.839 217 91 1 50 259 1 217 1.57e-83 251
MS.gene002639.t1 AT4G19030 68.519 162 44 1 27 181 44 205 2.35e-75 229
MS.gene002639.t1 AT2G34390 54.128 218 82 3 1 201 1 217 1.31e-68 212
MS.gene002639.t1 AT4G10380 47.755 245 113 4 27 259 63 304 1.46e-67 212
MS.gene002639.t1 AT1G80760 52.036 221 101 2 34 252 76 293 1.73e-67 212
MS.gene002639.t1 AT2G34390 52.500 200 77 3 1 183 1 199 1.59e-59 189
MS.gene002639.t1 AT3G06100 40.367 218 128 2 37 253 45 261 2.09e-44 151
MS.gene002639.t1 AT3G06100 40.654 214 125 2 41 253 1 213 5.01e-44 149
MS.gene002639.t1 AT1G31885 56.842 95 41 0 20 114 25 119 2.28e-31 114
MS.gene002639.t1 AT2G29870 48.718 117 60 0 153 269 20 136 1.47e-30 111
MS.gene002639.t1 AT3G16240 34.783 207 128 3 37 236 18 224 3.32e-28 108
MS.gene002639.t1 AT5G47450 34.199 231 142 6 37 257 18 248 9.06e-27 105
MS.gene002639.t1 AT4G17340 33.478 230 143 6 37 256 18 247 8.31e-25 99.8
MS.gene002639.t1 AT3G06100 39.716 141 83 2 114 253 41 180 8.84e-25 98.2
MS.gene002639.t1 AT1G17810 32.794 247 143 7 24 253 13 253 2.29e-23 96.3
MS.gene002639.t1 AT1G73190 30.556 252 140 7 24 253 13 251 7.80e-22 92.4
MS.gene002639.t1 AT2G25810 30.837 227 137 7 19 237 7 221 5.78e-19 84.0
MS.gene002639.t1 AT3G26520 32.381 210 129 5 37 236 21 227 2.97e-18 82.0
MS.gene002639.t1 AT4G35100 30.131 229 137 6 38 247 38 262 1.36e-17 80.9
MS.gene002639.t1 AT4G35100 30.131 229 137 6 38 247 38 262 1.36e-17 80.9
MS.gene002639.t1 AT5G60660 28.270 237 143 6 35 247 36 269 2.10e-17 80.5
MS.gene002639.t1 AT2G36830 32.301 226 136 7 26 240 12 231 2.20e-17 79.7
MS.gene002639.t1 AT2G16850 28.017 232 138 6 38 247 36 260 3.59e-16 77.0
MS.gene002639.t1 AT2G39010 31.217 189 116 5 72 247 81 268 9.08e-16 75.9
MS.gene002639.t1 AT4G01470 30.288 208 138 3 37 237 20 227 1.08e-15 75.1
MS.gene002639.t1 AT3G53420 29.319 191 117 4 72 247 82 269 1.56e-15 75.1
MS.gene002639.t1 AT3G53420 29.319 191 117 4 72 247 82 269 1.56e-15 75.1
MS.gene002639.t1 AT1G01620 31.492 181 106 4 72 237 17 194 1.13e-14 71.6
MS.gene002639.t1 AT3G54820 26.923 234 144 5 38 247 38 268 1.15e-14 72.8
MS.gene002639.t1 AT1G01620 31.492 181 106 4 72 237 89 266 1.97e-14 72.0
MS.gene002639.t1 AT2G37170 28.272 191 119 4 72 247 80 267 3.36e-14 71.2
MS.gene002639.t1 AT3G61430 27.830 212 138 5 41 237 55 266 4.08e-14 71.2
MS.gene002639.t1 AT3G61430 27.830 212 138 5 41 237 55 266 4.08e-14 71.2
MS.gene002639.t1 AT4G00430 31.492 181 106 4 72 237 90 267 5.96e-14 70.9
MS.gene002639.t1 AT2G37170 28.272 191 119 4 72 247 123 310 6.22e-14 71.2
MS.gene002639.t1 AT2G37180 28.272 191 119 4 72 247 80 267 7.04e-14 70.5
MS.gene002639.t1 AT3G47440 29.630 216 142 5 37 244 22 235 9.51e-14 69.7
MS.gene002639.t1 AT2G45960 27.830 212 138 5 41 237 55 266 1.20e-13 69.7
MS.gene002639.t1 AT4G23400 30.939 181 107 4 72 237 90 267 1.80e-13 69.3
MS.gene002639.t1 AT2G45960 28.351 194 127 4 41 222 55 248 7.45e-13 67.8
MS.gene002639.t1 AT2G45960 28.351 194 127 4 41 222 55 248 7.88e-13 67.4

Find 74 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTCTGTTGGTCACATTTC+TGG 0.071303 2.1:-68853898 MS.gene002639:CDS
TATCTTATGTTCGTCATTTC+TGG 0.082482 2.1:-68853063 MS.gene002639:CDS
AAGAGTGCCTCTTTCCTTAA+AGG 0.183929 2.1:-68851976 MS.gene002639:CDS
TCCTGGGATTTCAATTGTTT+GGG 0.185357 2.1:-68853944 MS.gene002639:CDS
TTCATTGATGCTCCAGTAAT+TGG 0.244218 2.1:+68852147 None:intergenic
TAGAGGAATATGGATATATT+TGG 0.245829 2.1:-68852082 MS.gene002639:CDS
ATCCTGGGATTTCAATTGTT+TGG 0.255375 2.1:-68853945 MS.gene002639:CDS
GGGTCTCACTGTTATGGTTT+TGG 0.266153 2.1:-68853923 MS.gene002639:CDS
TGCATGACTCATCACAGTTT+TGG 0.271846 2.1:+68854445 None:intergenic
TGCATCCACCAAAAGGTTTC+CGG 0.271933 2.1:-68853842 MS.gene002639:CDS
TTCCTTAAAGGAGTAGAAAA+TGG 0.281136 2.1:-68851964 MS.gene002639:CDS
ACGTACTTCTTGATATTTGC+AGG 0.286199 2.1:-68854015 MS.gene002639:CDS
TTTGTTAACAGGCCAATTAC+TGG 0.289918 2.1:-68852159 MS.gene002639:intron
CTGCTTAACCGGAAACCTTT+TGG 0.301395 2.1:+68853834 None:intergenic
TGTTTGGGGTCTCACTGTTA+TGG 0.308145 2.1:-68853929 MS.gene002639:CDS
TATATAATATCTCAAGTTCT+TGG 0.312865 2.1:-68853207 MS.gene002639:CDS
CTTCTAAATGTTATGTTTGC+CGG 0.328211 2.1:-68852530 MS.gene002639:CDS
GCAGGTCCTAAGCTCCTTGC+AGG 0.331255 2.1:+68852123 None:intergenic
ATAAAGAGTTTAGAGGAATA+TGG 0.344662 2.1:-68852092 MS.gene002639:CDS
TTACATTCAAAACCATTTCA+TGG 0.353026 2.1:+68854481 None:intergenic
ACTCGGATCACCTGCTTAAC+CGG 0.396444 2.1:+68853823 None:intergenic
TCCTGCTGGTTCTGATCTCC+AGG 0.406259 2.1:-68853115 MS.gene002639:CDS
ATTGTTCATAAAGAGTTTAG+AGG 0.413252 2.1:-68852099 MS.gene002639:CDS
TTCTGATCTCCAGGCATTTG+TGG 0.415818 2.1:-68853106 MS.gene002639:CDS
TTCTACTCCTTTAAGGAAAG+AGG 0.436744 2.1:+68851969 None:intergenic
ATGAACCCTGCAAGGAGCTT+AGG 0.445185 2.1:-68852129 MS.gene002639:CDS
AGTAGATCCAACTGCAAGTC+CGG 0.451623 2.1:+68852556 None:intergenic
ACACTTAGGCTTATATTCAA+TGG 0.454254 2.1:-68853165 MS.gene002639:CDS
GACAAAGTGGTAACACATCC+TGG 0.456746 2.1:-68853961 MS.gene002639:CDS
CAAAAGGTTTCCGGTTAAGC+AGG 0.462415 2.1:-68853833 MS.gene002639:intron
CTCTTACTAATTTCACGCAC+TGG 0.467398 2.1:+68851994 None:intergenic
AAGCAATGGAAACCATGAAA+TGG 0.472703 2.1:-68854493 MS.gene002639:CDS
GCAAAAGAGGCACATAGTCT+TGG 0.488743 2.1:+68854421 None:intergenic
ACAACTCTTGGTGCCATAGC+TGG 0.490463 2.1:-68852054 MS.gene002639:CDS
TTGGTGCCATAGCTGGTACA+TGG 0.491349 2.1:-68852047 MS.gene002639:CDS
ATACACACCTTCTGCAAAAG+AGG 0.497346 2.1:+68854408 None:intergenic
TTCTAAATGTTATGTTTGCC+GGG 0.499731 2.1:-68852529 MS.gene002639:intron
TATTTGGTATCAACAACTCT+TGG 0.500417 2.1:-68852066 MS.gene002639:CDS
AACTCTTTATGAACAATAGC+AGG 0.503476 2.1:+68852105 None:intergenic
TGCAGGGTGTGCTGCTGTTG+TGG 0.511373 2.1:-68853998 MS.gene002639:CDS
TTGCTCATGCATCCACCAAA+AGG 0.514247 2.1:-68853849 MS.gene002639:CDS
CCTGGGATTTCAATTGTTTG+GGG 0.520676 2.1:-68853943 MS.gene002639:CDS
TGAATTCAACCACAAATGCC+TGG 0.521494 2.1:+68853097 None:intergenic
TTACCACTTTGTCATTGTCA+AGG 0.527510 2.1:+68853971 None:intergenic
GGAGATTACCGCTCTATTGT+CGG 0.528942 2.1:+68853032 None:intergenic
GTAGTTGGTAGCAGAGGTTG+TGG 0.535028 2.1:-68854040 MS.gene002639:intron
TGGTGCCATAGCTGGTACAT+GGG 0.538881 2.1:-68852046 MS.gene002639:CDS
TCGCAGATTGGTGAGTTGGC+CGG 0.540241 2.1:-68852575 MS.gene002639:intron
CCCCAAACAATTGAAATCCC+AGG 0.550515 2.1:+68853943 None:intergenic
TATGTTCGTCATTTCTGGAG+TGG 0.553193 2.1:-68853058 MS.gene002639:CDS
GCATGAGCAATGGTGACAGC+AGG 0.555499 2.1:+68853862 None:intergenic
TAGTTGGTAGCAGAGGTTGT+GGG 0.558311 2.1:-68854039 MS.gene002639:CDS
AATATCGCAGATTGGTGAGT+TGG 0.558536 2.1:-68852579 MS.gene002639:intron
GCACTGGCTTGTTTGTGTAC+CGG 0.558893 2.1:+68852010 None:intergenic
TAGGCTTATATTCAATGGCA+AGG 0.559061 2.1:-68853160 MS.gene002639:CDS
CATGGGCATATACTTTCATC+CGG 0.565944 2.1:-68852029 MS.gene002639:CDS
CTTGGATCAACTCTTGCAAG+TGG 0.567738 2.1:-68853189 MS.gene002639:CDS
CTTAAAGGAGTAGAAAATGG+TGG 0.573302 2.1:-68851961 MS.gene002639:CDS
CGTACTTCTTGATATTTGCA+GGG 0.575700 2.1:-68854014 MS.gene002639:CDS
GCCTGGAGATCAGAACCAGC+AGG 0.579301 2.1:+68853114 None:intergenic
ATATGCCCATGTACCAGCTA+TGG 0.580208 2.1:+68852041 None:intergenic
ACTTGAGATATTATATAAGC+CGG 0.584944 2.1:+68853213 None:intergenic
GAGTTGGCCGGACTTGCAGT+TGG 0.596194 2.1:-68852563 MS.gene002639:CDS
GCTGATCATTCATCAAGCAA+TGG 0.606516 2.1:-68854507 MS.gene002639:CDS
ACAAAGTGGTAACACATCCT+GGG 0.613941 2.1:-68853960 MS.gene002639:CDS
GAGCATCAATGAACCCTGCA+AGG 0.617643 2.1:-68852137 MS.gene002639:CDS
CAGGTCCTAAGCTCCTTGCA+GGG 0.629880 2.1:+68852124 None:intergenic
CTCTTGCAAGTGGAACACTT+AGG 0.640873 2.1:-68853179 MS.gene002639:CDS
TTGAATGTAGTTGGTAGCAG+AGG 0.649135 2.1:-68854046 MS.gene002639:intron
AGTTGGTAGCAGAGGTTGTG+GGG 0.653045 2.1:-68854038 MS.gene002639:CDS
TTTGGTGGATGCATGAGCAA+TGG 0.663155 2.1:+68853852 None:intergenic
GATTACCGCTCTATTGTCGG+TGG 0.674328 2.1:+68853035 None:intergenic
GAACCTTGACAATGACAAAG+TGG 0.704582 2.1:-68853974 MS.gene002639:CDS
AGTGGCCACCGACAATAGAG+CGG 0.719424 2.1:-68853040 MS.gene002639:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTGACAAAAAAAATAAA+AGG + chr2.1:68853867-68853886 None:intergenic 10.0%
!!! AACAAATTTTTTATTTTCAA+CGG + chr2.1:68853517-68853536 None:intergenic 10.0%
!!! TTATTCAATAATTTTTTAGA+TGG - chr2.1:68852837-68852856 MS.gene002639:intron 10.0%
!!! TTATTTTTCTTTTGAAAATT+GGG - chr2.1:68853761-68853780 MS.gene002639:intron 10.0%
!!! TTTATTTTTCTTTTGAAAAT+TGG - chr2.1:68853760-68853779 MS.gene002639:intron 10.0%
!! GAGTATTTGTTTATATATAA+GGG + chr2.1:68853459-68853478 None:intergenic 15.0%
!! TAAAGAGTAAAATACTTTAA+AGG - chr2.1:68852313-68852332 MS.gene002639:intron 15.0%
!!! AGAGTATTTGTTTATATATA+AGG + chr2.1:68853460-68853479 None:intergenic 15.0%
!!! ATCTTTGTTATTAAAGTATT+AGG + chr2.1:68852229-68852248 None:intergenic 15.0%
!!! GAATTTAATCTCTAAAATAT+CGG - chr2.1:68852127-68852146 MS.gene002639:CDS 15.0%
!!! TACTTTAAAGGTTAAATTAA+CGG - chr2.1:68852325-68852344 MS.gene002639:intron 15.0%
!! AAGAAAATATGATTGCTTTA+AGG + chr2.1:68852924-68852943 None:intergenic 20.0%
!! AATGTATCTGATTCAAAATT+TGG - chr2.1:68852721-68852740 MS.gene002639:intron 20.0%
!! ACATGTTAATGAATATAATG+TGG - chr2.1:68853731-68853750 MS.gene002639:intron 20.0%
!! ATAAACAAATACTCTTGTAT+TGG - chr2.1:68853465-68853484 MS.gene002639:intron 20.0%
!! CCATATTATGAATTCAAAAA+CGG + chr2.1:68853091-68853110 None:intergenic 20.0%
!! GAATCAATGTTGAAATAAAA+TGG - chr2.1:68852376-68852395 MS.gene002639:intron 20.0%
!! GTACAATTAAGTATACTATT+TGG + chr2.1:68853174-68853193 None:intergenic 20.0%
!! TATATAATATCTCAAGTTCT+TGG - chr2.1:68853254-68853273 MS.gene002639:intron 20.0%
!! TTTGGATAATATGCAAAAAA+TGG + chr2.1:68853156-68853175 None:intergenic 20.0%
!!! CTGATATGTGATTTTAAATA+TGG - chr2.1:68852354-68852373 MS.gene002639:intron 20.0%
!!! GAATATAGTTTTAAAGAACT+CGG + chr2.1:68852658-68852677 None:intergenic 20.0%
! AAATGACATAATATAGAGCA+TGG + chr2.1:68853051-68853070 None:intergenic 25.0%
! ACTTGAGATATTATATAAGC+CGG + chr2.1:68853251-68853270 None:intergenic 25.0%
! ATAAAGAGTTTAGAGGAATA+TGG - chr2.1:68854369-68854388 MS.gene002639:intron 25.0%
! ATTGTTCATAAAGAGTTTAG+AGG - chr2.1:68854362-68854381 MS.gene002639:intron 25.0%
! GCAAAAAATGGAAAAAGTTT+AGG + chr2.1:68853144-68853163 None:intergenic 25.0%
! TAATCTTATTGAAGAACTCT+CGG + chr2.1:68854214-68854233 None:intergenic 25.0%
! TAGAGGAATATGGATATATT+TGG - chr2.1:68854379-68854398 MS.gene002639:intron 25.0%
! TCTAACATTTACTCTCTTAT+TGG + chr2.1:68854129-68854148 None:intergenic 25.0%
! TGTATTATGTTGAATGTAGT+TGG - chr2.1:68852406-68852425 MS.gene002639:intron 25.0%
! TTACATTCAAAACCATTTCA+TGG + chr2.1:68851983-68852002 None:intergenic 25.0%
! TTGTTTATATATAAGGGCAA+AGG + chr2.1:68853453-68853472 None:intergenic 25.0%
!! AATCCTATATGTTTGTTAAC+AGG - chr2.1:68854291-68854310 MS.gene002639:intron 25.0%
!! TTCATTAACATGTGTTTCTA+AGG + chr2.1:68853724-68853743 None:intergenic 25.0%
!! TTGAGGTTTGATTTATAGTT+AGG + chr2.1:68853964-68853983 None:intergenic 25.0%
!!! CAATAATTTTTTAGATGGTG+TGG - chr2.1:68852842-68852861 MS.gene002639:intron 25.0%
!!! CCGTTTTTGAATTCATAATA+TGG - chr2.1:68853088-68853107 MS.gene002639:CDS 25.0%
!!! GAAATGGTTTTGAATGTAAA+TGG - chr2.1:68851984-68852003 MS.gene002639:CDS 25.0%
!!! TTCTCAATATGTTTAGTTTG+AGG + chr2.1:68853981-68854000 None:intergenic 25.0%
AAAACGGTTGCATACATATA+CGG + chr2.1:68853075-68853094 None:intergenic 30.0%
AACAATCACTCTCTTATTGA+GGG - chr2.1:68854035-68854054 MS.gene002639:CDS 30.0%
AACTCTTTATGAACAATAGC+AGG + chr2.1:68854359-68854378 None:intergenic 30.0%
AATCCATATGCTTCAAAGTT+GGG + chr2.1:68852771-68852790 None:intergenic 30.0%
AATTGCAAATATGTCACAAG+TGG - chr2.1:68853606-68853625 MS.gene002639:intron 30.0%
ACACTTAGGCTTATATTCAA+TGG - chr2.1:68853296-68853315 MS.gene002639:intron 30.0%
CAAAAAAGTTCTATGTAGAG+TGG + chr2.1:68852087-68852106 None:intergenic 30.0%
CATTCCTCAAATATAGTTAG+TGG - chr2.1:68854177-68854196 MS.gene002639:intron 30.0%
CTTCTAAATGTTATGTTTGC+CGG - chr2.1:68853931-68853950 MS.gene002639:CDS 30.0%
GCTTTATATGCGAAAAAGTT+GGG - chr2.1:68852686-68852705 MS.gene002639:intron 30.0%
GGAAGAGAAATTTCAATTGA+AGG + chr2.1:68853123-68853142 None:intergenic 30.0%
TAACAATCACTCTCTTATTG+AGG - chr2.1:68854034-68854053 MS.gene002639:CDS 30.0%
TAAGAGAGTAAATGTTAGAG+AGG - chr2.1:68854130-68854149 MS.gene002639:intron 30.0%
TAATCCATATGCTTCAAAGT+TGG + chr2.1:68852772-68852791 None:intergenic 30.0%
TAGAGAGGACATTAGAAAAA+AGG - chr2.1:68854145-68854164 MS.gene002639:intron 30.0%
TATCTTATGTTCGTCATTTC+TGG - chr2.1:68853398-68853417 MS.gene002639:intron 30.0%
TATTTGGTATCAACAACTCT+TGG - chr2.1:68854395-68854414 MS.gene002639:intron 30.0%
TCTTCAATCTATAATCTTGC+AGG - chr2.1:68853223-68853242 MS.gene002639:intron 30.0%
TGCTTTATATGCGAAAAAGT+TGG - chr2.1:68852685-68852704 MS.gene002639:intron 30.0%
TTATTGGATCAAACTCACAT+GGG + chr2.1:68854113-68854132 None:intergenic 30.0%
TTCCTTAAAGGAGTAGAAAA+TGG - chr2.1:68854497-68854516 MS.gene002639:CDS 30.0%
TTCTAAATGTTATGTTTGCC+GGG - chr2.1:68853932-68853951 MS.gene002639:CDS 30.0%
TTTATAGTTAGGTTCATACC+CGG + chr2.1:68853953-68853972 None:intergenic 30.0%
! ACAATAGTGTTGAAGATTGA+TGG + chr2.1:68853662-68853681 None:intergenic 30.0%
! TTTTTGTCAATATCGCAGAT+TGG - chr2.1:68853874-68853893 MS.gene002639:CDS 30.0%
AAATATGTCACAAGTGGACA+TGG - chr2.1:68853612-68853631 MS.gene002639:intron 35.0%
AAGCAATGGAAACCATGAAA+TGG - chr2.1:68851968-68851987 MS.gene002639:CDS 35.0%
AATATGTCACAAGTGGACAT+GGG - chr2.1:68853613-68853632 MS.gene002639:intron 35.0%
ACGTACTTCTTGATATTTGC+AGG - chr2.1:68852446-68852465 MS.gene002639:intron 35.0%
AGTTCTATGTAGAGTGGTTT+AGG + chr2.1:68852081-68852100 None:intergenic 35.0%
ATCCTGGGATTTCAATTGTT+TGG - chr2.1:68852516-68852535 MS.gene002639:intron 35.0%
ATCTATAATCTTGCAGGTAC+CGG - chr2.1:68853229-68853248 MS.gene002639:intron 35.0%
CGTACTTCTTGATATTTGCA+GGG - chr2.1:68852447-68852466 MS.gene002639:intron 35.0%
CTTAAAGGAGTAGAAAATGG+TGG - chr2.1:68854500-68854519 MS.gene002639:CDS 35.0%
CTTATTGGATCAAACTCACA+TGG + chr2.1:68854114-68854133 None:intergenic 35.0%
GTGTCCACTAACTATATTTG+AGG + chr2.1:68854184-68854203 None:intergenic 35.0%
TAGGCTTATATTCAATGGCA+AGG - chr2.1:68853301-68853320 MS.gene002639:intron 35.0%
TCCTGGGATTTCAATTGTTT+GGG - chr2.1:68852517-68852536 MS.gene002639:intron 35.0%
TGGCCTGTTAACAAACATAT+AGG + chr2.1:68854297-68854316 None:intergenic 35.0%
TTACCACTTTGTCATTGTCA+AGG + chr2.1:68852493-68852512 None:intergenic 35.0%
TTCATTGATGCTCCAGTAAT+TGG + chr2.1:68854317-68854336 None:intergenic 35.0%
TTTGTTAACAGGCCAATTAC+TGG - chr2.1:68854302-68854321 MS.gene002639:intron 35.0%
! CACCATTTTCTACTCCTTTA+AGG + chr2.1:68854502-68854521 None:intergenic 35.0%
! CATATTAACACGTGTCGTTA+AGG + chr2.1:68853843-68853862 None:intergenic 35.0%
! TTCTACTCCTTTAAGGAAAG+AGG + chr2.1:68854495-68854514 None:intergenic 35.0%
!!! ATGGTTTTGGTTTACTCTGT+TGG - chr2.1:68852551-68852570 MS.gene002639:CDS 35.0%
AATATCGCAGATTGGTGAGT+TGG - chr2.1:68853882-68853901 MS.gene002639:CDS 40.0%
ACAAAGTGGTAACACATCCT+GGG - chr2.1:68852501-68852520 MS.gene002639:intron 40.0%
ATACACACCTTCTGCAAAAG+AGG + chr2.1:68852056-68852075 None:intergenic 40.0%
CATGGGCATATACTTTCATC+CGG - chr2.1:68854432-68854451 MS.gene002639:CDS 40.0%
CATGTCGTCACAATTCAGTT+TGG - chr2.1:68854238-68854257 MS.gene002639:intron 40.0%
CCTGGGATTTCAATTGTTTG+GGG - chr2.1:68852518-68852537 MS.gene002639:intron 40.0%
CTCTTACTAATTTCACGCAC+TGG + chr2.1:68854470-68854489 None:intergenic 40.0%
GAACCTTGACAATGACAAAG+TGG - chr2.1:68852487-68852506 MS.gene002639:intron 40.0%
GAATTGGGTATCACCTAAAG+TGG + chr2.1:68854078-68854097 None:intergenic 40.0%
GATGTTATGCGTGACTATGA+TGG - chr2.1:68852807-68852826 MS.gene002639:intron 40.0%
GCTGATCATTCATCAAGCAA+TGG - chr2.1:68851954-68851973 MS.gene002639:CDS 40.0%
TACTCTGTTGGTCACATTTC+TGG - chr2.1:68852563-68852582 MS.gene002639:CDS 40.0%
TCTTATTGAGGGAACTCATG+TGG - chr2.1:68854046-68854065 MS.gene002639:intron 40.0%
TGAATTCAACCACAAATGCC+TGG + chr2.1:68853367-68853386 None:intergenic 40.0%
TTCGCATATAAAGCACACGA+CGG + chr2.1:68852680-68852699 None:intergenic 40.0%
! GCATGACTCATCACAGTTTT+GGG + chr2.1:68852018-68852037 None:intergenic 40.0%
! TATGTTCGTCATTTCTGGAG+TGG - chr2.1:68853403-68853422 MS.gene002639:intron 40.0%
! TGCATGACTCATCACAGTTT+TGG + chr2.1:68852019-68852038 None:intergenic 40.0%
!! AAGAGTGCCTCTTTCCTTAA+AGG - chr2.1:68854485-68854504 MS.gene002639:CDS 40.0%
!! CAGGAAGTGTTCCTGAAAAA+TGG + chr2.1:68853331-68853350 None:intergenic 40.0%
!! GGCAAGGAAAACCATTTTTC+AGG - chr2.1:68853317-68853336 MS.gene002639:intron 40.0%
!! TGACCCAACTTTGAAGCATA+TGG - chr2.1:68852765-68852784 MS.gene002639:intron 40.0%
!! TTGAATGTAGTTGGTAGCAG+AGG - chr2.1:68852415-68852434 MS.gene002639:intron 40.0%
AGTAGATCCAACTGCAAGTC+CGG + chr2.1:68853908-68853927 None:intergenic 45.0%
ATATGCCCATGTACCAGCTA+TGG + chr2.1:68854423-68854442 None:intergenic 45.0%
CAAAAGGTTTCCGGTTAAGC+AGG - chr2.1:68852628-68852647 MS.gene002639:intron 45.0%
CATGTGGATCACACCACTTT+AGG - chr2.1:68854062-68854081 MS.gene002639:intron 45.0%
CCCCAAACAATTGAAATCCC+AGG + chr2.1:68852521-68852540 None:intergenic 45.0%
CTCTTGCAAGTGGAACACTT+AGG - chr2.1:68853282-68853301 MS.gene002639:intron 45.0%
CTTGGATCAACTCTTGCAAG+TGG - chr2.1:68853272-68853291 MS.gene002639:intron 45.0%
GACAAAGTGGTAACACATCC+TGG - chr2.1:68852500-68852519 MS.gene002639:intron 45.0%
GGAGATTACCGCTCTATTGT+CGG + chr2.1:68853432-68853451 None:intergenic 45.0%
TGCATCCACCAAAAGGTTTC+CGG - chr2.1:68852619-68852638 MS.gene002639:intron 45.0%
TTCTGATCTCCAGGCATTTG+TGG - chr2.1:68853355-68853374 MS.gene002639:intron 45.0%
TTGCTCATGCATCCACCAAA+AGG - chr2.1:68852612-68852631 MS.gene002639:intron 45.0%
! CTATGTGCCTCTTTTGCAGA+AGG - chr2.1:68852046-68852065 MS.gene002639:CDS 45.0%
! CTGCTTAACCGGAAACCTTT+TGG + chr2.1:68852630-68852649 None:intergenic 45.0%
! CTTAACCGGAAACCTTTTGG+TGG + chr2.1:68852627-68852646 None:intergenic 45.0%
! GCAAAAGAGGCACATAGTCT+TGG + chr2.1:68852043-68852062 None:intergenic 45.0%
! GGGTCTCACTGTTATGGTTT+TGG - chr2.1:68852538-68852557 MS.gene002639:CDS 45.0%
! TGTTTGGGGTCTCACTGTTA+TGG - chr2.1:68852532-68852551 MS.gene002639:CDS 45.0%
! TTTGGTGGATGCATGAGCAA+TGG + chr2.1:68852612-68852631 None:intergenic 45.0%
!! GGGTCTCACACTTTGGAATT+GGG + chr2.1:68854093-68854112 None:intergenic 45.0%
!! TAGTTGGTAGCAGAGGTTGT+GGG - chr2.1:68852422-68852441 MS.gene002639:intron 45.0%
!! TGGGTCTCACACTTTGGAAT+TGG + chr2.1:68854094-68854113 None:intergenic 45.0%
!! TTTTCAGGAACACTTCCTGC+TGG - chr2.1:68853332-68853351 MS.gene002639:intron 45.0%
ACTCGGATCACCTGCTTAAC+CGG + chr2.1:68852641-68852660 None:intergenic 50.0%
ATGAACCCTGCAAGGAGCTT+AGG - chr2.1:68854332-68854351 MS.gene002639:intron 50.0%
CTCACATGGGTCTCACACTT+TGG + chr2.1:68854100-68854119 None:intergenic 50.0%
GAGCATCAATGAACCCTGCA+AGG - chr2.1:68854324-68854343 MS.gene002639:intron 50.0%
GATTACCGCTCTATTGTCGG+TGG + chr2.1:68853429-68853448 None:intergenic 50.0%
!! ACAACTCTTGGTGCCATAGC+TGG - chr2.1:68854407-68854426 MS.gene002639:intron 50.0%
!! AGTTGGTAGCAGAGGTTGTG+GGG - chr2.1:68852423-68852442 MS.gene002639:intron 50.0%
!! GCACTGGCTTGTTTGTGTAC+CGG + chr2.1:68854454-68854473 None:intergenic 50.0%
!! GTAGTTGGTAGCAGAGGTTG+TGG - chr2.1:68852421-68852440 MS.gene002639:intron 50.0%
!! TGGTGCCATAGCTGGTACAT+GGG - chr2.1:68854415-68854434 MS.gene002639:intron 50.0%
!! TTGGTGCCATAGCTGGTACA+TGG - chr2.1:68854414-68854433 MS.gene002639:intron 50.0%
AGTGGCCACCGACAATAGAG+CGG - chr2.1:68853421-68853440 MS.gene002639:intron 55.0%
CAGGTCCTAAGCTCCTTGCA+GGG + chr2.1:68854340-68854359 None:intergenic 55.0%
GCATGAGCAATGGTGACAGC+AGG + chr2.1:68852602-68852621 None:intergenic 55.0%
! TCGCAGATTGGTGAGTTGGC+CGG - chr2.1:68853886-68853905 MS.gene002639:CDS 55.0%
!! TCCTGCTGGTTCTGATCTCC+AGG - chr2.1:68853346-68853365 MS.gene002639:intron 55.0%
GAGTTGGCCGGACTTGCAGT+TGG - chr2.1:68853898-68853917 MS.gene002639:CDS 60.0%
GCAGGTCCTAAGCTCCTTGC+AGG + chr2.1:68854341-68854360 None:intergenic 60.0%
GCCTGGAGATCAGAACCAGC+AGG + chr2.1:68853350-68853369 None:intergenic 60.0%
! TGCAGGGTGTGCTGCTGTTG+TGG - chr2.1:68852463-68852482 MS.gene002639:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 68851951 68854532 68851951 ID=MS.gene002639
chr2.1 mRNA 68851951 68854532 68851951 ID=MS.gene002639.t1;Parent=MS.gene002639
chr2.1 exon 68854416 68854532 68854416 ID=MS.gene002639.t1.exon1;Parent=MS.gene002639.t1
chr2.1 CDS 68854416 68854532 68854416 ID=cds.MS.gene002639.t1;Parent=MS.gene002639.t1
chr2.1 exon 68853834 68854058 68853834 ID=MS.gene002639.t1.exon2;Parent=MS.gene002639.t1
chr2.1 CDS 68853834 68854058 68853834 ID=cds.MS.gene002639.t1;Parent=MS.gene002639.t1
chr2.1 exon 68853041 68853238 68853041 ID=MS.gene002639.t1.exon3;Parent=MS.gene002639.t1
chr2.1 CDS 68853041 68853238 68853041 ID=cds.MS.gene002639.t1;Parent=MS.gene002639.t1
chr2.1 exon 68852530 68852591 68852530 ID=MS.gene002639.t1.exon4;Parent=MS.gene002639.t1
chr2.1 CDS 68852530 68852591 68852530 ID=cds.MS.gene002639.t1;Parent=MS.gene002639.t1
chr2.1 exon 68851951 68852170 68851951 ID=MS.gene002639.t1.exon5;Parent=MS.gene002639.t1
chr2.1 CDS 68851951 68852170 68851951 ID=cds.MS.gene002639.t1;Parent=MS.gene002639.t1
Gene Sequence

>MS.gene002639

ATGGCTGATCATTCATCAAGCAATGGAAACCATGAAATGGTTTTGAATGTAAATGGTGATGCTTCCCAAAACTGTGATGAGTCATGCATCCAAGACTATGTGCCTCTTTTGCAGAAGGTGTGTATCTCCTAAACCACTCTACATAGAACTTTTTTGTTGATAATAACTATGAATATGAATTTAATCTCTAAAATATCGGTCTCTAATTTTATTTTTGAAGTAAATAAAATTCGTTGTCATAGTCTCTATAGTATATGAAAGTTATTATATCAATTCCTAATACTTTAATAACAAAGATTAAAGTGACGAATTTCATATACTTAAAAAAAGTTTATTACTTTTATATGCTTTAAAGATTTAATTAAAGAGTAAAATACTTTAAAGGTTAAATTAACGGTTTATTCTGATATGTGATTTTAAATATGGAATCAATGTTGAAATAAAATGGTTTGATTTGTATTATGTTGAATGTAGTTGGTAGCAGAGGTTGTGGGGACGTACTTCTTGATATTTGCAGGGTGTGCTGCTGTTGTGGTGAACCTTGACAATGACAAAGTGGTAACACATCCTGGGATTTCAATTGTTTGGGGTCTCACTGTTATGGTTTTGGTTTACTCTGTTGGTCACATTTCTGGTGCTCATTTCAATCCTGCTGTCACCATTGCTCATGCATCCACCAAAAGGTTTCCGGTTAAGCAGGTGATCCGAGTTCTTTAAAACTATATTCCGTCGTGTGCTTTATATGCGAAAAAGTTGGGTCAACAAAAATTAATGTATCTGATTCAAAATTTGGATCAAATACATAAATTTTTACTGACCCAACTTTGAAGCATATGGATTATTTTATTTTGAAAACGATGTTATGCGTGACTATGATGGTACACATTTATTCAATAATTTTTTAGATGGTGTGGTAAATGAAAATATAATTATTACACTTGAATTTTCTTGAAAATTACTTGTGTTAGCTCCTTAAAGCAATCATATTTTCTTTAAAGAAAATGTTAACAAATGCTCTAAAAGCACTTGATAAGAAGTTTAAAGTAGAATTTTTTTTCTATAAGATTGATTTTACATATAAAAAAGCAAAAGCTAAACCATGCTCTATATTATGTCATTTTCCGTATATGTATGCAACCGTTTTTGAATTCATAATATGGTTGAATTGACCTTCAATTGAAATTTCTCTTCCTAAACTTTTTCCATTTTTTGCATATTATCCAAATAGTATACTTAATTGTACTAGTTTGTCTAAAAAAATTGTTGAGTTTTTCTTCAATCTATAATCTTGCAGGTACCGGCTTATATAATATCTCAAGTTCTTGGATCAACTCTTGCAAGTGGAACACTTAGGCTTATATTCAATGGCAAGGAAAACCATTTTTCAGGAACACTTCCTGCTGGTTCTGATCTCCAGGCATTTGTGGTTGAATTCATAATCACTTTTTATCTTATGTTCGTCATTTCTGGAGTGGCCACCGACAATAGAGCGGTAATCTCCTTTGCCCTTATATATAAACAAATACTCTTGTATTGGAAAGTGTTGAAATATTGTATATTCAACCGTTGAAAATAAAAAATTTGTTATTTTCAATGAAAAGTTTTTTACTTTCTTATATTCTTAACTAATTAGCATGACTCTTAAAATATGTATAAATTGCAAATATGTCACAAGTGGACATGGGCAGCAAATTCTACATTTCAATGTCCATCAATCTTCAACACTATTGTATACTAGATTTTGTTATTTTAGTGAAATGCAAACATGCGCCTTAGAAACACATGTTAATGAATATAATGTGGATATTTTTTATTTTTCTTTTGAAAATTGGGCATTTAATTTTTTAAATATTTAAAAGTTGTTTTCTGCAATGTAAATGTTCTACATGCCTTAACGACACGTGTTAATATGACCTTTTATTTTTTTTGTCAATATCGCAGATTGGTGAGTTGGCCGGACTTGCAGTTGGATCTACTGTGCTTCTAAATGTTATGTTTGCCGGGTATGAACCTAACTATAAATCAAACCTCAAACTAAACATATTGAGAAATGCTAGAAACATTCGCTCTCTAACACTATTTTTAACAATCACTCTCTTATTGAGGGAACTCATGTGGATCACACCACTTTAGGTGATACCCAATTCCAAAGTGTGAGACCCATGTGAGTTTGATCCAATAAGAGAGTAAATGTTAGAGAGGACATTAGAAAAAAGGTGCTCAGATCATTCCTCAAATATAGTTAGTGGACACTTTTACACCGAGAGTTCTTCAATAAGATTACAATCATGTCGTCACAATTCAGTTTGGTCAAATTACTTAGAACTTAATCATGCATGCAATCCTATATGTTTGTTAACAGGCCAATTACTGGAGCATCAATGAACCCTGCAAGGAGCTTAGGACCTGCTATTGTTCATAAAGAGTTTAGAGGAATATGGATATATTTGGTATCAACAACTCTTGGTGCCATAGCTGGTACATGGGCATATACTTTCATCCGGTACACAAACAAGCCAGTGCGTGAAATTAGTAAGAGTGCCTCTTTCCTTAAAGGAGTAGAAAATGGTGGAGCTAAGTGA
Protein sequence

>MS.gene002639.t1

MADHSSSNGNHEMVLNVNGDASQNCDESCIQDYVPLLQKLVAEVVGTYFLIFAGCAAVVVNLDNDKVVTHPGISIVWGLTVMVLVYSVGHISGAHFNPAVTIAHASTKRFPVKQVPAYIISQVLGSTLASGTLRLIFNGKENHFSGTLPAGSDLQAFVVEFIITFYLMFVISGVATDNRAIGELAGLAVGSTVLLNVMFAGPITGASMNPARSLGPAIVHKEFRGIWIYLVSTTLGAIAGTWAYTFIRYTNKPVREISKSASFLKGVENGGAK