AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01624

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01624.t1 MTR_2g017570 97.436 273 7 0 1 273 1 273 0.0 545
MS.gene01624.t1 MTR_2g017610 97.436 273 7 0 1 273 1 273 0.0 543
MS.gene01624.t1 MTR_2g017590 81.439 264 49 0 7 270 5 268 9.08e-164 455
MS.gene01624.t1 MTR_2g017620 78.571 266 57 0 1 266 1 266 3.95e-155 433
MS.gene01624.t1 MTR_8g087720 76.866 268 62 0 1 268 1 268 1.42e-153 429
MS.gene01624.t1 MTR_2g017620 79.762 252 51 0 15 266 1 252 3.16e-149 417
MS.gene01624.t1 MTR_8g087710 70.480 271 80 0 3 273 4 274 2.95e-142 400
MS.gene01624.t1 MTR_2g017660 69.485 272 76 2 1 265 1 272 1.79e-140 396
MS.gene01624.t1 MTR_8g087720 78.109 201 44 0 1 201 1 201 1.02e-111 320
MS.gene01624.t1 MTR_2g017620 82.703 185 32 0 82 266 1 185 7.27e-111 318
MS.gene01624.t1 MTR_4g084880 58.025 243 100 1 32 272 29 271 1.50e-102 300
MS.gene01624.t1 MTR_8g087720 75.000 184 46 0 1 184 1 184 4.69e-97 283
MS.gene01624.t1 MTR_5g079570 52.720 239 104 3 18 253 22 254 2.80e-84 253
MS.gene01624.t1 MTR_3g008920 53.556 239 108 2 18 256 8 243 9.51e-84 252
MS.gene01624.t1 MTR_4g006730 49.402 251 118 3 21 265 58 305 2.05e-70 219
MS.gene01624.t1 MTR_7g010650 47.085 223 115 2 31 251 28 249 8.87e-69 214
MS.gene01624.t1 MTR_2g104940 51.163 215 100 2 36 248 78 289 1.53e-67 212
MS.gene01624.t1 MTR_5g063930 46.947 262 116 7 15 260 5 259 3.33e-67 209
MS.gene01624.t1 MTR_1g097840 46.800 250 114 4 26 259 54 300 3.30e-66 208
MS.gene01624.t1 MTR_4g006630 50.226 221 101 3 51 265 1 218 4.25e-64 200
MS.gene01624.t1 MTR_4g006730 50.226 221 101 3 51 265 1 218 5.34e-64 200
MS.gene01624.t1 MTR_4g006730 50.820 183 85 2 21 201 58 237 5.21e-47 158
MS.gene01624.t1 MTR_1g070385 39.906 213 127 1 40 252 64 275 1.76e-44 160
MS.gene01624.t1 MTR_1g097840 47.849 186 84 3 26 201 54 236 1.25e-43 149
MS.gene01624.t1 MTR_7g010650 43.137 153 84 2 31 181 28 179 1.70e-37 131
MS.gene01624.t1 MTR_4g006650 47.222 144 62 3 39 180 5 136 6.71e-27 107
MS.gene01624.t1 MTR_8g013680 32.850 207 132 3 37 236 18 224 1.43e-25 101
MS.gene01624.t1 MTR_1g077540 31.950 241 146 6 24 253 10 243 2.03e-24 99.0
MS.gene01624.t1 MTR_2g101370 30.242 248 161 6 18 256 3 247 8.37e-23 94.4
MS.gene01624.t1 MTR_5g012810 33.913 230 141 7 37 256 18 246 1.41e-22 93.6
MS.gene01624.t1 MTR_6g069040 29.583 240 153 6 24 253 10 243 3.16e-22 92.8
MS.gene01624.t1 MTR_1g006490 32.143 224 136 7 19 236 7 220 4.15e-22 92.4
MS.gene01624.t1 MTR_1g006590 32.000 250 150 9 19 258 7 246 2.33e-21 90.5
MS.gene01624.t1 MTR_4g063090 32.159 227 140 7 43 256 26 251 2.34e-17 79.7
MS.gene01624.t1 MTR_7g103030 30.667 225 146 6 37 252 20 243 3.57e-17 79.0
MS.gene01624.t1 MTR_3g118010 28.384 229 141 5 38 247 37 261 1.88e-16 77.8
MS.gene01624.t1 MTR_2g094270 30.899 178 111 3 72 237 84 261 2.11e-16 77.4
MS.gene01624.t1 MTR_5g082070 31.019 216 127 7 41 237 58 270 4.06e-16 76.6
MS.gene01624.t1 MTR_5g010150 30.047 213 133 5 41 237 55 267 1.24e-15 75.5
MS.gene01624.t1 MTR_7g101190 29.412 221 135 6 38 237 39 259 1.28e-15 75.5
MS.gene01624.t1 MTR_8g098375 30.516 213 132 6 41 237 55 267 1.54e-15 75.1
MS.gene01624.t1 MTR_1g115405 32.227 211 130 5 37 237 20 227 1.58e-15 74.7
MS.gene01624.t1 MTR_3g070210 28.638 213 136 5 41 237 58 270 1.11e-14 72.8
MS.gene01624.t1 MTR_4g415300 29.245 212 135 5 41 237 59 270 2.15e-14 72.0
MS.gene01624.t1 MTR_2g094270 30.573 157 100 2 72 219 84 240 7.55e-14 70.1
MS.gene01624.t1 MTR_3g070210 28.205 195 127 4 41 222 58 252 2.72e-13 68.6
MS.gene01624.t1 MTR_1g095070 28.571 182 112 4 81 247 25 203 3.05e-13 67.8
MS.gene01624.t1 MTR_7g023350 28.740 254 147 9 11 251 4 236 7.53e-13 67.0
MS.gene01624.t1 MTR_2g060360 26.639 244 164 5 37 273 18 253 9.83e-13 66.6
MS.gene01624.t1 MTR_4g059390 29.282 181 110 5 72 237 82 259 7.15e-12 64.7
MS.gene01624.t1 MTR_1g095070 28.571 154 95 3 81 222 25 175 4.28e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01624.t1 AT4G18910 70.817 257 68 1 24 273 38 294 1.39e-131 374
MS.gene01624.t1 AT4G19030 68.504 254 73 1 24 270 41 294 1.48e-125 359
MS.gene01624.t1 AT5G37820 58.431 255 103 1 18 269 20 274 1.12e-99 293
MS.gene01624.t1 AT5G37820 58.431 255 103 1 18 269 56 310 4.46e-99 293
MS.gene01624.t1 AT5G37810 54.015 274 119 3 1 268 1 273 3.52e-98 289
MS.gene01624.t1 AT4G18910 75.135 185 39 1 24 201 38 222 2.17e-92 272
MS.gene01624.t1 AT2G34390 51.748 286 120 3 1 269 1 285 1.93e-90 270
MS.gene01624.t1 AT1G31885 53.036 247 109 1 20 259 25 271 3.33e-90 270
MS.gene01624.t1 AT4G19030 70.109 184 48 1 24 200 41 224 6.09e-88 261
MS.gene01624.t1 AT2G34390 55.462 238 98 2 39 269 7 243 4.47e-86 257
MS.gene01624.t1 AT1G31885 54.839 217 91 1 50 259 1 217 1.61e-83 251
MS.gene01624.t1 AT4G19030 67.879 165 46 1 24 181 41 205 8.26e-76 230
MS.gene01624.t1 AT2G34390 54.128 218 82 3 1 201 1 217 8.86e-69 212
MS.gene01624.t1 AT4G10380 47.755 245 113 4 27 259 63 304 1.22e-67 212
MS.gene01624.t1 AT1G80760 52.036 221 101 2 34 252 76 293 1.29e-67 212
MS.gene01624.t1 AT2G34390 52.500 200 77 3 1 183 1 199 9.86e-60 189
MS.gene01624.t1 AT3G06100 40.367 218 128 2 37 253 45 261 2.07e-44 151
MS.gene01624.t1 AT3G06100 40.654 214 125 2 41 253 1 213 4.65e-44 149
MS.gene01624.t1 AT1G31885 56.842 95 41 0 20 114 25 119 2.94e-31 113
MS.gene01624.t1 AT2G29870 48.718 117 60 0 153 269 20 136 1.45e-30 111
MS.gene01624.t1 AT3G16240 34.783 207 128 3 37 236 18 224 3.47e-28 108
MS.gene01624.t1 AT5G47450 34.199 231 142 6 37 257 18 248 8.96e-27 105
MS.gene01624.t1 AT4G17340 33.478 230 143 6 37 256 18 247 8.14e-25 99.8
MS.gene01624.t1 AT3G06100 39.716 141 83 2 114 253 41 180 9.12e-25 98.2
MS.gene01624.t1 AT1G17810 32.794 247 143 7 24 253 13 253 7.85e-24 97.8
MS.gene01624.t1 AT1G73190 30.556 252 140 7 24 253 13 251 2.50e-22 93.6
MS.gene01624.t1 AT2G25810 30.837 227 137 7 19 237 7 221 4.09e-19 84.3
MS.gene01624.t1 AT3G26520 32.381 210 129 5 37 236 21 227 2.66e-18 82.4
MS.gene01624.t1 AT2G36830 32.018 228 138 7 24 240 10 231 1.12e-17 80.5
MS.gene01624.t1 AT4G35100 30.131 229 137 6 38 247 38 262 1.33e-17 80.9
MS.gene01624.t1 AT4G35100 30.131 229 137 6 38 247 38 262 1.33e-17 80.9
MS.gene01624.t1 AT5G60660 28.270 237 143 6 35 247 36 269 2.04e-17 80.5
MS.gene01624.t1 AT2G16850 28.017 232 138 6 38 247 36 260 3.85e-16 76.6
MS.gene01624.t1 AT2G39010 31.217 189 116 5 72 247 81 268 8.90e-16 75.9
MS.gene01624.t1 AT4G01470 35.065 154 97 2 87 237 74 227 1.05e-15 75.1
MS.gene01624.t1 AT3G53420 29.319 191 117 4 72 247 82 269 1.50e-15 75.1
MS.gene01624.t1 AT3G53420 29.319 191 117 4 72 247 82 269 1.50e-15 75.1
MS.gene01624.t1 AT3G54820 26.923 234 144 5 38 247 38 268 1.02e-14 72.8
MS.gene01624.t1 AT1G01620 31.492 181 106 4 72 237 17 194 1.10e-14 71.6
MS.gene01624.t1 AT1G01620 31.492 181 106 4 72 237 89 266 2.04e-14 72.0
MS.gene01624.t1 AT2G37170 28.272 191 119 4 72 247 80 267 3.33e-14 71.2
MS.gene01624.t1 AT3G61430 27.830 212 138 5 41 237 55 266 3.63e-14 71.2
MS.gene01624.t1 AT3G61430 27.830 212 138 5 41 237 55 266 3.63e-14 71.2
MS.gene01624.t1 AT4G00430 31.492 181 106 4 72 237 90 267 5.26e-14 70.9
MS.gene01624.t1 AT2G37170 28.272 191 119 4 72 247 123 310 5.87e-14 71.2
MS.gene01624.t1 AT2G37180 28.272 191 119 4 72 247 80 267 6.97e-14 70.5
MS.gene01624.t1 AT3G47440 29.630 216 142 5 37 244 22 235 7.90e-14 70.1
MS.gene01624.t1 AT2G45960 27.830 212 138 5 41 237 55 266 9.60e-14 70.1
MS.gene01624.t1 AT4G23400 30.939 181 107 4 72 237 90 267 1.68e-13 69.3
MS.gene01624.t1 AT2G45960 28.351 194 127 4 41 222 55 248 6.82e-13 67.4
MS.gene01624.t1 AT2G45960 28.351 194 127 4 41 222 55 248 6.96e-13 67.8

Find 74 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTCTGTTGGTCACATTTC+TGG 0.071303 2.2:-66787432 MS.gene01624:CDS
TATCTTATGTTCGTCATTTC+TGG 0.082482 2.2:-66786597 MS.gene01624:CDS
AAGAGTGCCTCTTTCCTTAA+AGG 0.183929 2.2:-66785479 MS.gene01624:CDS
TCCTGGGATTTCAATTGTTT+GGG 0.185357 2.2:-66787478 MS.gene01624:CDS
TTCATTGATGCTCCAGTAAT+TGG 0.244218 2.2:+66785650 None:intergenic
TAGAGGAATATGGATATATT+TGG 0.245829 2.2:-66785585 MS.gene01624:CDS
TGCATGACTCATCACACTTT+TGG 0.246215 2.2:+66787953 None:intergenic
ATCCTGGGATTTCAATTGTT+TGG 0.255375 2.2:-66787479 MS.gene01624:CDS
GGGTCTCACTGTTATGGTTT+TGG 0.266153 2.2:-66787457 MS.gene01624:CDS
TGCATCCACCAAAAGGTTTC+CGG 0.271933 2.2:-66787376 MS.gene01624:CDS
TTCCTTAAAGGAGTAGAAAA+TGG 0.281136 2.2:-66785467 MS.gene01624:CDS
ACGTACTTCTTGATATTTGC+AGG 0.286199 2.2:-66787549 MS.gene01624:CDS
TTTGTTAACAGGCCAATTAC+TGG 0.289918 2.2:-66785662 MS.gene01624:intron
CTGCTTAACCGGAAACCTTT+TGG 0.301395 2.2:+66787368 None:intergenic
TGTTTGGGGTCTCACTGTTA+TGG 0.308145 2.2:-66787463 MS.gene01624:CDS
TATATAATATCTCAAGTTCT+TGG 0.312865 2.2:-66786741 MS.gene01624:CDS
CTTCTAAATGTTATGTTTGC+CGG 0.328211 2.2:-66786037 MS.gene01624:CDS
GCAGGTCCTAAGCTCCTTGC+AGG 0.331255 2.2:+66785626 None:intergenic
ATAAAGAGTTTAGAGGAATA+TGG 0.344662 2.2:-66785595 MS.gene01624:CDS
TTACATTCAAAACCATTTCA+TGG 0.353026 2.2:+66787989 None:intergenic
ACTCGGATCACCTGCTTAAC+CGG 0.396444 2.2:+66787357 None:intergenic
TCCTGCTGGTTCTGATCTCC+AGG 0.406259 2.2:-66786649 MS.gene01624:CDS
ATTGTTCATAAAGAGTTTAG+AGG 0.413252 2.2:-66785602 MS.gene01624:CDS
TTCTGATCTCCAGGCATTTG+TGG 0.415818 2.2:-66786640 MS.gene01624:CDS
TTCTACTCCTTTAAGGAAAG+AGG 0.436744 2.2:+66785472 None:intergenic
ATGAACCCTGCAAGGAGCTT+AGG 0.445185 2.2:-66785632 MS.gene01624:CDS
AGTAGATCCAACTGCAAGTC+CGG 0.451623 2.2:+66786063 None:intergenic
ACACTTAGGCTTATATTCAA+TGG 0.454254 2.2:-66786699 MS.gene01624:CDS
GACAAAGTGGTAACACATCC+TGG 0.456746 2.2:-66787495 MS.gene01624:CDS
CAAAAGGTTTCCGGTTAAGC+AGG 0.462415 2.2:-66787367 MS.gene01624:intron
CTCTTACTAATTTCACGCAC+TGG 0.467398 2.2:+66785497 None:intergenic
AAGCAATGGAAACCATGAAA+TGG 0.472703 2.2:-66788001 MS.gene01624:CDS
GCAAAAGAGGCACATAGTCT+TGG 0.488743 2.2:+66787929 None:intergenic
ACAACTCTTGGTGCCATAGC+TGG 0.490463 2.2:-66785557 MS.gene01624:CDS
TTGGTGCCATAGCTGGTACA+TGG 0.491349 2.2:-66785550 MS.gene01624:CDS
ATACACACCTTCTGCAAAAG+AGG 0.497346 2.2:+66787916 None:intergenic
TTCTAAATGTTATGTTTGCC+GGG 0.499731 2.2:-66786036 MS.gene01624:intron
TATTTGGTATCAACAACTCT+TGG 0.500417 2.2:-66785569 MS.gene01624:CDS
AACTCTTTATGAACAATAGC+AGG 0.503476 2.2:+66785608 None:intergenic
TGCAGGGTGTGCTGCTGTTG+TGG 0.511373 2.2:-66787532 MS.gene01624:CDS
TTGCTCATGCATCCACCAAA+AGG 0.514247 2.2:-66787383 MS.gene01624:CDS
CCTGGGATTTCAATTGTTTG+GGG 0.520676 2.2:-66787477 MS.gene01624:CDS
TGAATTCAACCACAAATGCC+TGG 0.521494 2.2:+66786631 None:intergenic
TTACCACTTTGTCATTGTCA+AGG 0.527510 2.2:+66787505 None:intergenic
GGAGATTACCGCTCTATTGT+CGG 0.528942 2.2:+66786566 None:intergenic
GTAGTTGGTAGCAGAGGTTG+TGG 0.535028 2.2:-66787574 MS.gene01624:intron
TGGTGCCATAGCTGGTACAT+GGG 0.538881 2.2:-66785549 MS.gene01624:CDS
TCGCAGATTGGTGAGTTGGC+CGG 0.540241 2.2:-66786082 MS.gene01624:intron
CCCCAAACAATTGAAATCCC+AGG 0.550515 2.2:+66787477 None:intergenic
TATGTTCGTCATTTCTGGAG+TGG 0.553193 2.2:-66786592 MS.gene01624:CDS
GCATGAGCAATGGTGACAGC+AGG 0.555499 2.2:+66787396 None:intergenic
TAGTTGGTAGCAGAGGTTGT+GGG 0.558311 2.2:-66787573 MS.gene01624:CDS
AATATCGCAGATTGGTGAGT+TGG 0.558536 2.2:-66786086 MS.gene01624:intron
GCACTGGCTTGTTTGTGTAC+CGG 0.558893 2.2:+66785513 None:intergenic
TAGGCTTATATTCAATGGCA+AGG 0.559061 2.2:-66786694 MS.gene01624:CDS
CATGGGCATATACTTTCATC+CGG 0.565944 2.2:-66785532 MS.gene01624:CDS
CTTGGATCAACTCTTGCAAG+TGG 0.567738 2.2:-66786723 MS.gene01624:CDS
CTTAAAGGAGTAGAAAATGG+TGG 0.573302 2.2:-66785464 MS.gene01624:CDS
CGTACTTCTTGATATTTGCA+GGG 0.575700 2.2:-66787548 MS.gene01624:CDS
GCCTGGAGATCAGAACCAGC+AGG 0.579301 2.2:+66786648 None:intergenic
ATATGCCCATGTACCAGCTA+TGG 0.580208 2.2:+66785544 None:intergenic
ACTTGAGATATTATATAAGC+CGG 0.584944 2.2:+66786747 None:intergenic
GAGTTGGCCGGACTTGCAGT+TGG 0.596194 2.2:-66786070 MS.gene01624:CDS
GCTGATCATTCATCAAGCAA+TGG 0.606516 2.2:-66788015 MS.gene01624:CDS
ACAAAGTGGTAACACATCCT+GGG 0.613941 2.2:-66787494 MS.gene01624:CDS
GAGCATCAATGAACCCTGCA+AGG 0.617643 2.2:-66785640 MS.gene01624:CDS
CAGGTCCTAAGCTCCTTGCA+GGG 0.629880 2.2:+66785627 None:intergenic
CTCTTGCAAGTGGAACACTT+AGG 0.640873 2.2:-66786713 MS.gene01624:CDS
TTGAATGTAGTTGGTAGCAG+AGG 0.649135 2.2:-66787580 MS.gene01624:intron
AGTTGGTAGCAGAGGTTGTG+GGG 0.653045 2.2:-66787572 MS.gene01624:CDS
TTTGGTGGATGCATGAGCAA+TGG 0.663155 2.2:+66787386 None:intergenic
GATTACCGCTCTATTGTCGG+TGG 0.674328 2.2:+66786569 None:intergenic
GAACCTTGACAATGACAAAG+TGG 0.704582 2.2:-66787508 MS.gene01624:CDS
AGTGGCCACCGACAATAGAG+CGG 0.719424 2.2:-66786574 MS.gene01624:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGACAAAAAAAAATAAA+AGG + chr2.2:66787370-66787389 None:intergenic 10.0%
!!! TTATTCAATAATTTTTTAGA+TGG - chr2.2:66786314-66786333 MS.gene01624:intron 10.0%
!!! TTATTTTTCTTTTGAAAATT+GGG - chr2.2:66787264-66787283 MS.gene01624:intron 10.0%
!!! TTTATTTTTCTTTTGAAAAT+TGG - chr2.2:66787263-66787282 MS.gene01624:intron 10.0%
!!! TTTTTTTAAGTATATGAAAT+TGG + chr2.2:66785763-66785782 None:intergenic 10.0%
!! GAGTATTTGTTTATATATAA+GGG + chr2.2:66786936-66786955 None:intergenic 15.0%
!!! AGAGTATTTGTTTATATATA+AGG + chr2.2:66786937-66786956 None:intergenic 15.0%
!!! ATCTTTGTTATTAAAGTATT+AGG + chr2.2:66785732-66785751 None:intergenic 15.0%
!!! GAATTTAATCTCTAAAATAT+CGG - chr2.2:66785630-66785649 MS.gene01624:CDS 15.0%
!! AAGAAAATATGATTGCTTTA+AGG + chr2.2:66786401-66786420 None:intergenic 20.0%
!! AATGTATCTGATTCAAAATT+TGG - chr2.2:66786198-66786217 MS.gene01624:intron 20.0%
!! ACATGTTAATGAATATAATG+TGG - chr2.2:66787234-66787253 MS.gene01624:intron 20.0%
!! ATAAACAAATACTCTTGTAT+TGG - chr2.2:66786942-66786961 MS.gene01624:intron 20.0%
!! CCATATTATGAATTCAAAAA+CGG + chr2.2:66786568-66786587 None:intergenic 20.0%
!! GAATCAATGTTGAAATAAAA+TGG - chr2.2:66785853-66785872 MS.gene01624:intron 20.0%
!! GTACAATTAAGTATACTATT+TGG + chr2.2:66786651-66786670 None:intergenic 20.0%
!! TATATAATATCTCAAGTTCT+TGG - chr2.2:66786731-66786750 MS.gene01624:CDS 20.0%
!! TTTGGATAATATGCAAAAAA+TGG + chr2.2:66786633-66786652 None:intergenic 20.0%
!!! AACAATTTTTTTAGACAAAC+TGG + chr2.2:66786673-66786692 None:intergenic 20.0%
!!! CTGATATGTGATTTTAAATA+TGG - chr2.2:66785831-66785850 MS.gene01624:intron 20.0%
!!! GAATATAGTTTTAAAGAACT+CGG + chr2.2:66786135-66786154 None:intergenic 20.0%
! AAATGACATAATATAGAGCA+TGG + chr2.2:66786528-66786547 None:intergenic 25.0%
! ACTTGAGATATTATATAAGC+CGG + chr2.2:66786728-66786747 None:intergenic 25.0%
! ATAAAGAGTTTAGAGGAATA+TGG - chr2.2:66787877-66787896 MS.gene01624:intron 25.0%
! ATTGTTCATAAAGAGTTTAG+AGG - chr2.2:66787870-66787889 MS.gene01624:intron 25.0%
! GCAAAAAATGGAAAAAGTTT+AGG + chr2.2:66786621-66786640 None:intergenic 25.0%
! TAATCTTATTGAAGAACTCT+CGG + chr2.2:66787722-66787741 None:intergenic 25.0%
! TAGAGGAATATGGATATATT+TGG - chr2.2:66787887-66787906 MS.gene01624:intron 25.0%
! TGTATTATGTTGAATGTAGT+TGG - chr2.2:66785883-66785902 MS.gene01624:intron 25.0%
! TTACATTCAAAACCATTTCA+TGG + chr2.2:66785486-66785505 None:intergenic 25.0%
! TTATTAGATCAAACTCACAT+GGG + chr2.2:66787621-66787640 None:intergenic 25.0%
! TTGTTTATATATAAGGGCAA+AGG + chr2.2:66786930-66786949 None:intergenic 25.0%
!! AATCCTATATGTTTGTTAAC+AGG - chr2.2:66787799-66787818 MS.gene01624:intron 25.0%
!! GAATATACACAACTTTTCAA+CGG + chr2.2:66786994-66787013 None:intergenic 25.0%
!! TTCATTAACATGTGTTTCTA+AGG + chr2.2:66787227-66787246 None:intergenic 25.0%
!! TTGAGGTTTGATTTATAGTT+AGG + chr2.2:66787468-66787487 None:intergenic 25.0%
!!! CAATAATTTTTTAGATGGTG+TGG - chr2.2:66786319-66786338 MS.gene01624:intron 25.0%
!!! CCGTTTTTGAATTCATAATA+TGG - chr2.2:66786565-66786584 MS.gene01624:intron 25.0%
!!! GAAATGGTTTTGAATGTAAA+TGG - chr2.2:66785487-66785506 MS.gene01624:CDS 25.0%
!!! TTCTCAATATGTTTAGTTTG+AGG + chr2.2:66787485-66787504 None:intergenic 25.0%
AAAACGGTTGCATACATATA+CGG + chr2.2:66786552-66786571 None:intergenic 30.0%
AACAATCACTCTCTTATTGA+GGG - chr2.2:66787543-66787562 MS.gene01624:CDS 30.0%
AACTCTTTATGAACAATAGC+AGG + chr2.2:66787867-66787886 None:intergenic 30.0%
AATCCATATGCTTCAAAGTT+GGG + chr2.2:66786248-66786267 None:intergenic 30.0%
AATTGCAAATATGTCACAAG+TGG - chr2.2:66787109-66787128 MS.gene01624:intron 30.0%
ACACTTAGGCTTATATTCAA+TGG - chr2.2:66786773-66786792 MS.gene01624:intron 30.0%
CAAAAAAGTTCTATGTAGAG+TGG + chr2.2:66785590-66785609 None:intergenic 30.0%
CATTCCTCAAATATAGTTAG+TGG - chr2.2:66787685-66787704 MS.gene01624:intron 30.0%
CTTATTAGATCAAACTCACA+TGG + chr2.2:66787622-66787641 None:intergenic 30.0%
CTTCTAAATGTTATGTTTGC+CGG - chr2.2:66787435-66787454 MS.gene01624:CDS 30.0%
GGAAGAGAAATTTCAATTGA+AGG + chr2.2:66786600-66786619 None:intergenic 30.0%
TAACAATCACTCTCTTATTG+AGG - chr2.2:66787542-66787561 MS.gene01624:CDS 30.0%
TAAGAGAGTAAATGTTAGAG+AGG - chr2.2:66787638-66787657 MS.gene01624:intron 30.0%
TAATCCATATGCTTCAAAGT+TGG + chr2.2:66786249-66786268 None:intergenic 30.0%
TAGAGAGGACATTAGAAAAA+AGG - chr2.2:66787653-66787672 MS.gene01624:intron 30.0%
TATCTTATGTTCGTCATTTC+TGG - chr2.2:66786875-66786894 MS.gene01624:intron 30.0%
TATTTGGTATCAACAACTCT+TGG - chr2.2:66787903-66787922 MS.gene01624:intron 30.0%
TCTTCAATCTATAATCTTGC+AGG - chr2.2:66786700-66786719 MS.gene01624:CDS 30.0%
TTCCTTAAAGGAGTAGAAAA+TGG - chr2.2:66788005-66788024 MS.gene01624:CDS 30.0%
TTCTAAATGTTATGTTTGCC+GGG - chr2.2:66787436-66787455 MS.gene01624:CDS 30.0%
TTTATAGTTAGGTTCATACC+CGG + chr2.2:66787457-66787476 None:intergenic 30.0%
! ACAATAGTGTTGAAGATTGA+TGG + chr2.2:66787165-66787184 None:intergenic 30.0%
! TTTTTGTCAATATCGCAGAT+TGG - chr2.2:66787378-66787397 MS.gene01624:CDS 30.0%
AAATATGTCACAAGTGGACA+TGG - chr2.2:66787115-66787134 MS.gene01624:intron 35.0%
AAGCAATGGAAACCATGAAA+TGG - chr2.2:66785471-66785490 MS.gene01624:CDS 35.0%
AATATGTCACAAGTGGACAT+GGG - chr2.2:66787116-66787135 MS.gene01624:intron 35.0%
ACGTACTTCTTGATATTTGC+AGG - chr2.2:66785923-66785942 MS.gene01624:intron 35.0%
AGTTCTATGTAGAGTGGTTT+AGG + chr2.2:66785584-66785603 None:intergenic 35.0%
ATCCTGGGATTTCAATTGTT+TGG - chr2.2:66785993-66786012 MS.gene01624:intron 35.0%
ATCTATAATCTTGCAGGTAC+CGG - chr2.2:66786706-66786725 MS.gene01624:CDS 35.0%
CGTACTTCTTGATATTTGCA+GGG - chr2.2:66785924-66785943 MS.gene01624:intron 35.0%
CTTAAAGGAGTAGAAAATGG+TGG - chr2.2:66788008-66788027 MS.gene01624:CDS 35.0%
GTGTCCACTAACTATATTTG+AGG + chr2.2:66787692-66787711 None:intergenic 35.0%
TAGGCTTATATTCAATGGCA+AGG - chr2.2:66786778-66786797 MS.gene01624:intron 35.0%
TCCTGGGATTTCAATTGTTT+GGG - chr2.2:66785994-66786013 MS.gene01624:intron 35.0%
TGGCCTGTTAACAAACATAT+AGG + chr2.2:66787805-66787824 None:intergenic 35.0%
TTACCACTTTGTCATTGTCA+AGG + chr2.2:66785970-66785989 None:intergenic 35.0%
TTCATTGATGCTCCAGTAAT+TGG + chr2.2:66787825-66787844 None:intergenic 35.0%
TTTGTTAACAGGCCAATTAC+TGG - chr2.2:66787810-66787829 MS.gene01624:intron 35.0%
! CACCATTTTCTACTCCTTTA+AGG + chr2.2:66788010-66788029 None:intergenic 35.0%
! CATATTAACACGTGTCGTTA+AGG + chr2.2:66787346-66787365 None:intergenic 35.0%
! TTCTACTCCTTTAAGGAAAG+AGG + chr2.2:66788003-66788022 None:intergenic 35.0%
!!! ATGGTTTTGGTTTACTCTGT+TGG - chr2.2:66786028-66786047 MS.gene01624:intron 35.0%
AATATCGCAGATTGGTGAGT+TGG - chr2.2:66787386-66787405 MS.gene01624:CDS 40.0%
ACAAAGTGGTAACACATCCT+GGG - chr2.2:66785978-66785997 MS.gene01624:intron 40.0%
ATACACACCTTCTGCAAAAG+AGG + chr2.2:66785559-66785578 None:intergenic 40.0%
CATGGGCATATACTTTCATC+CGG - chr2.2:66787940-66787959 MS.gene01624:CDS 40.0%
CATGTCGTCACAATTCAGTT+TGG - chr2.2:66787746-66787765 MS.gene01624:intron 40.0%
CCTGGGATTTCAATTGTTTG+GGG - chr2.2:66785995-66786014 MS.gene01624:intron 40.0%
CTCTTACTAATTTCACGCAC+TGG + chr2.2:66787978-66787997 None:intergenic 40.0%
GAACCTTGACAATGACAAAG+TGG - chr2.2:66785964-66785983 MS.gene01624:intron 40.0%
GAATTGGGTATCACCTAAAG+TGG + chr2.2:66787586-66787605 None:intergenic 40.0%
GATGTTATGCGTGACTATGA+TGG - chr2.2:66786284-66786303 MS.gene01624:intron 40.0%
GCTGATCATTCATCAAGCAA+TGG - chr2.2:66785457-66785476 MS.gene01624:CDS 40.0%
TACTCTGTTGGTCACATTTC+TGG - chr2.2:66786040-66786059 MS.gene01624:CDS 40.0%
TCTTATTGAGGGAACTCATG+TGG - chr2.2:66787554-66787573 MS.gene01624:CDS 40.0%
TGAATTCAACCACAAATGCC+TGG + chr2.2:66786844-66786863 None:intergenic 40.0%
TTCGCATATAAAGCACACGA+CGG + chr2.2:66786157-66786176 None:intergenic 40.0%
! GCATGACTCATCACACTTTT+GGG + chr2.2:66785521-66785540 None:intergenic 40.0%
! TATGTTCGTCATTTCTGGAG+TGG - chr2.2:66786880-66786899 MS.gene01624:intron 40.0%
! TGCATGACTCATCACACTTT+TGG + chr2.2:66785522-66785541 None:intergenic 40.0%
!! AAGAGTGCCTCTTTCCTTAA+AGG - chr2.2:66787993-66788012 MS.gene01624:CDS 40.0%
!! AGACCCAACTTTGAAGCATA+TGG - chr2.2:66786242-66786261 MS.gene01624:intron 40.0%
!! CAGGAAGTGTTCCTGAAAAA+TGG + chr2.2:66786808-66786827 None:intergenic 40.0%
!! GGCAAGGAAAACCATTTTTC+AGG - chr2.2:66786794-66786813 MS.gene01624:intron 40.0%
!! TTGAATGTAGTTGGTAGCAG+AGG - chr2.2:66785892-66785911 MS.gene01624:intron 40.0%
AGTAGATCCAACTGCAAGTC+CGG + chr2.2:66787412-66787431 None:intergenic 45.0%
ATATGCCCATGTACCAGCTA+TGG + chr2.2:66787931-66787950 None:intergenic 45.0%
CAAAAGGTTTCCGGTTAAGC+AGG - chr2.2:66786105-66786124 MS.gene01624:intron 45.0%
CATGTGGATCACACCACTTT+AGG - chr2.2:66787570-66787589 MS.gene01624:CDS 45.0%
CCCCAAACAATTGAAATCCC+AGG + chr2.2:66785998-66786017 None:intergenic 45.0%
CTCTTGCAAGTGGAACACTT+AGG - chr2.2:66786759-66786778 MS.gene01624:intron 45.0%
CTTGGATCAACTCTTGCAAG+TGG - chr2.2:66786749-66786768 MS.gene01624:CDS 45.0%
GACAAAGTGGTAACACATCC+TGG - chr2.2:66785977-66785996 MS.gene01624:intron 45.0%
GGAGATTACCGCTCTATTGT+CGG + chr2.2:66786909-66786928 None:intergenic 45.0%
TGCATCCACCAAAAGGTTTC+CGG - chr2.2:66786096-66786115 MS.gene01624:intron 45.0%
TTCTGATCTCCAGGCATTTG+TGG - chr2.2:66786832-66786851 MS.gene01624:intron 45.0%
TTGCTCATGCATCCACCAAA+AGG - chr2.2:66786089-66786108 MS.gene01624:intron 45.0%
! CTATGTGCCTCTTTTGCAGA+AGG - chr2.2:66785549-66785568 MS.gene01624:CDS 45.0%
! CTGCTTAACCGGAAACCTTT+TGG + chr2.2:66786107-66786126 None:intergenic 45.0%
! CTTAACCGGAAACCTTTTGG+TGG + chr2.2:66786104-66786123 None:intergenic 45.0%
! GCAAAAGAGGCACATAGTCT+TGG + chr2.2:66785546-66785565 None:intergenic 45.0%
! GGGTCTCACTGTTATGGTTT+TGG - chr2.2:66786015-66786034 MS.gene01624:intron 45.0%
! TGTTTGGGGTCTCACTGTTA+TGG - chr2.2:66786009-66786028 MS.gene01624:intron 45.0%
! TTTGGTGGATGCATGAGCAA+TGG + chr2.2:66786089-66786108 None:intergenic 45.0%
!! GGGTCTCACACTTTGGAATT+GGG + chr2.2:66787601-66787620 None:intergenic 45.0%
!! TAGTTGGTAGCAGAGGTTGT+GGG - chr2.2:66785899-66785918 MS.gene01624:intron 45.0%
!! TGGGTCTCACACTTTGGAAT+TGG + chr2.2:66787602-66787621 None:intergenic 45.0%
!! TTTTCAGGAACACTTCCTGC+TGG - chr2.2:66786809-66786828 MS.gene01624:intron 45.0%
ACTCGGATCACCTGCTTAAC+CGG + chr2.2:66786118-66786137 None:intergenic 50.0%
ATGAACCCTGCAAGGAGCTT+AGG - chr2.2:66787840-66787859 MS.gene01624:intron 50.0%
CTCACATGGGTCTCACACTT+TGG + chr2.2:66787608-66787627 None:intergenic 50.0%
GAGCATCAATGAACCCTGCA+AGG - chr2.2:66787832-66787851 MS.gene01624:intron 50.0%
GATTACCGCTCTATTGTCGG+TGG + chr2.2:66786906-66786925 None:intergenic 50.0%
!! ACAACTCTTGGTGCCATAGC+TGG - chr2.2:66787915-66787934 MS.gene01624:intron 50.0%
!! AGTTGGTAGCAGAGGTTGTG+GGG - chr2.2:66785900-66785919 MS.gene01624:intron 50.0%
!! GCACTGGCTTGTTTGTGTAC+CGG + chr2.2:66787962-66787981 None:intergenic 50.0%
!! GTAGTTGGTAGCAGAGGTTG+TGG - chr2.2:66785898-66785917 MS.gene01624:intron 50.0%
!! TGGTGCCATAGCTGGTACAT+GGG - chr2.2:66787923-66787942 MS.gene01624:intron 50.0%
!! TTGGTGCCATAGCTGGTACA+TGG - chr2.2:66787922-66787941 MS.gene01624:intron 50.0%
AGTGGCCACCGACAATAGAG+CGG - chr2.2:66786898-66786917 MS.gene01624:intron 55.0%
CAGGTCCTAAGCTCCTTGCA+GGG + chr2.2:66787848-66787867 None:intergenic 55.0%
GCATGAGCAATGGTGACAGC+AGG + chr2.2:66786079-66786098 None:intergenic 55.0%
! TCGCAGATTGGTGAGTTGGC+CGG - chr2.2:66787390-66787409 MS.gene01624:CDS 55.0%
!! TCCTGCTGGTTCTGATCTCC+AGG - chr2.2:66786823-66786842 MS.gene01624:intron 55.0%
GAGTTGGCCGGACTTGCAGT+TGG - chr2.2:66787402-66787421 MS.gene01624:CDS 60.0%
GCAGGTCCTAAGCTCCTTGC+AGG + chr2.2:66787849-66787868 None:intergenic 60.0%
GCCTGGAGATCAGAACCAGC+AGG + chr2.2:66786827-66786846 None:intergenic 60.0%
! TGCAGGGTGTGCTGCTGTTG+TGG - chr2.2:66785940-66785959 MS.gene01624:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 66785454 66788040 66785454 ID=MS.gene01624
chr2.2 mRNA 66785454 66788040 66785454 ID=MS.gene01624.t1;Parent=MS.gene01624
chr2.2 exon 66787924 66788040 66787924 ID=MS.gene01624.t1.exon1;Parent=MS.gene01624.t1
chr2.2 CDS 66787924 66788040 66787924 ID=cds.MS.gene01624.t1;Parent=MS.gene01624.t1
chr2.2 exon 66787368 66787592 66787368 ID=MS.gene01624.t1.exon2;Parent=MS.gene01624.t1
chr2.2 CDS 66787368 66787592 66787368 ID=cds.MS.gene01624.t1;Parent=MS.gene01624.t1
chr2.2 exon 66786575 66786772 66786575 ID=MS.gene01624.t1.exon3;Parent=MS.gene01624.t1
chr2.2 CDS 66786575 66786772 66786575 ID=cds.MS.gene01624.t1;Parent=MS.gene01624.t1
chr2.2 exon 66786037 66786098 66786037 ID=MS.gene01624.t1.exon4;Parent=MS.gene01624.t1
chr2.2 CDS 66786037 66786098 66786037 ID=cds.MS.gene01624.t1;Parent=MS.gene01624.t1
chr2.2 exon 66785454 66785673 66785454 ID=MS.gene01624.t1.exon5;Parent=MS.gene01624.t1
chr2.2 CDS 66785454 66785673 66785454 ID=cds.MS.gene01624.t1;Parent=MS.gene01624.t1
Gene Sequence

>MS.gene01624

ATGGCTGATCATTCATCAAGCAATGGAAACCATGAAATGGTTTTGAATGTAAATGGTGATGCTTCCCAAAAGTGTGATGAGTCATGCATCCAAGACTATGTGCCTCTTTTGCAGAAGGTGTGTATCTCCTAAACCACTCTACATAGAACTTTTTTGTTGATAATAACTATGAATATGAATTTAATCTCTAAAATATCGGTCTCTAATTTTATTTTTGAAGTAAATAAAATTCGTTGTCATAGTCTCTATAGTATATGAAAGTTATTATATCAATTCCTAATACTTTAATAACAAAGATTAAAGTGACCAATTTCATATACTTAAAAAAAGTTTATTACTTTTATATGCTTTAAAGATTTAATTAAAGTGACGAATTTCTGATATGTGATTTTAAATATGGAATCAATGTTGAAATAAAATGGTTTGATTTGTATTATGTTGAATGTAGTTGGTAGCAGAGGTTGTGGGGACGTACTTCTTGATATTTGCAGGGTGTGCTGCTGTTGTGGTGAACCTTGACAATGACAAAGTGGTAACACATCCTGGGATTTCAATTGTTTGGGGTCTCACTGTTATGGTTTTGGTTTACTCTGTTGGTCACATTTCTGGTGCTCATTTCAATCCTGCTGTCACCATTGCTCATGCATCCACCAAAAGGTTTCCGGTTAAGCAGGTGATCCGAGTTCTTTAAAACTATATTCCGTCGTGTGCTTTATATGCGAAAAAGTTGAGTCAACAAAAATTAATGTATCTGATTCAAAATTTGGATCAAATACATAAATTTTTACAGACCCAACTTTGAAGCATATGGATTATTTTATTTTGAAAACGATGTTATGCGTGACTATGATGGTACACATTTATTCAATAATTTTTTAGATGGTGTGGTAAATGAAAATATAATTATTACACTTGAATTTTCTTGAAAATTACTTGTGTTAGCTCCTTAAAGCAATCATATTTTCTTTAAAGAAAATGTTAACAAATGCTCTAAAAGCACTTGATAAGAAGTTTAAAGTAGAATTTTTTTTCTATAAGATTGATTTTACATATAAAAAAGCAAAAGCTAAACCATGCTCTATATTATGTCATTTTCCGTATATGTATGCAACCGTTTTTGAATTCATAATATGGTTGAATTGACCTTCAATTGAAATTTCTCTTCCTAAACTTTTTCCATTTTTTGCATATTATCCAAATAGTATACTTAATTGTACCAGTTTGTCTAAAAAAATTGTTGAGTTTTTCTTCAATCTATAATCTTGCAGGTACCGGCTTATATAATATCTCAAGTTCTTGGATCAACTCTTGCAAGTGGAACACTTAGGCTTATATTCAATGGCAAGGAAAACCATTTTTCAGGAACACTTCCTGCTGGTTCTGATCTCCAGGCATTTGTGGTTGAATTCATAATCACTTTTTATCTTATGTTCGTCATTTCTGGAGTGGCCACCGACAATAGAGCGGTAATCTCCTTTGCCCTTATATATAAACAAATACTCTTGTATTGGAAAGTGTTGAAATATTGTATATTCAACCGTTGAAAAGTTGTGTATATTCAACTTTTTGAAAATAAAAAATTTGTTATTTTCAATGAAAAGTTTTTTACTTTCTTATATTCTTAACTAATTAGCATGACTCTTAAAATATGTATAAATTGCAAATATGTCACAAGTGGACATGGGCAGCAAATTCTACATTTCAATGTCCATCAATCTTCAACACTATTGTATACTAGATTTTGTTATTTTAGTGAAATGCAAACATGCGCCTTAGAAACACATGTTAATGAATATAATGTGGATATTTTTTATTTTTCTTTTGAAAATTGGGCATTTAATTTTTTAAATATTTAAAAGTTGTTTTCTGCAATGTAAATGTTCTACATGCCTTAACGACACGTGTTAATATGACCTTTTATTTTTTTTTGTCAATATCGCAGATTGGTGAGTTGGCCGGACTTGCAGTTGGATCTACTGTGCTTCTAAATGTTATGTTTGCCGGGTATGAACCTAACTATAAATCAAACCTCAAACTAAACATATTGAGAAATGCTAGAAATTAACATTCGCTCTCTAACACTATTTTTAACAATCACTCTCTTATTGAGGGAACTCATGTGGATCACACCACTTTAGGTGATACCCAATTCCAAAGTGTGAGACCCATGTGAGTTTGATCTAATAAGAGAGTAAATGTTAGAGAGGACATTAGAAAAAAGGTGCTCAGATCATTCCTCAAATATAGTTAGTGGACACTTTTACACCGAGAGTTCTTCAATAAGATTACAATCATGTCGTCACAATTCAGTTTGGTCAAATTACTTAGAACTTAATCATGCATGCAATCCTATATGTTTGTTAACAGGCCAATTACTGGAGCATCAATGAACCCTGCAAGGAGCTTAGGACCTGCTATTGTTCATAAAGAGTTTAGAGGAATATGGATATATTTGGTATCAACAACTCTTGGTGCCATAGCTGGTACATGGGCATATACTTTCATCCGGTACACAAACAAGCCAGTGCGTGAAATTAGTAAGAGTGCCTCTTTCCTTAAAGGAGTAGAAAATGGTGGAGCTAAGTGA
Protein sequence

>MS.gene01624.t1

MADHSSSNGNHEMVLNVNGDASQKCDESCIQDYVPLLQKLVAEVVGTYFLIFAGCAAVVVNLDNDKVVTHPGISIVWGLTVMVLVYSVGHISGAHFNPAVTIAHASTKRFPVKQVPAYIISQVLGSTLASGTLRLIFNGKENHFSGTLPAGSDLQAFVVEFIITFYLMFVISGVATDNRAIGELAGLAVGSTVLLNVMFAGPITGASMNPARSLGPAIVHKEFRGIWIYLVSTTLGAIAGTWAYTFIRYTNKPVREISKSASFLKGVENGGAK