AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01625

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01625.t1 MTR_2g017590 95.572 271 11 1 1 271 1 270 0.0 521
MS.gene01625.t1 MTR_2g017620 90.000 270 25 1 1 268 1 270 5.47e-178 491
MS.gene01625.t1 MTR_2g017620 91.016 256 23 0 13 268 1 256 3.88e-172 475
MS.gene01625.t1 MTR_2g017570 80.150 267 53 0 5 271 7 273 3.37e-162 451
MS.gene01625.t1 MTR_2g017610 80.150 267 53 0 5 271 7 273 3.92e-162 451
MS.gene01625.t1 MTR_8g087720 75.862 261 63 0 5 265 7 267 1.33e-147 414
MS.gene01625.t1 MTR_2g017660 66.304 276 84 2 1 267 1 276 3.73e-134 380
MS.gene01625.t1 MTR_8g087710 67.557 262 85 0 10 271 13 274 7.50e-133 377
MS.gene01625.t1 MTR_2g017620 92.063 189 15 0 80 268 1 189 3.32e-125 354
MS.gene01625.t1 MTR_8g087720 75.385 195 48 0 5 199 7 201 2.34e-104 301
MS.gene01625.t1 MTR_4g084880 57.692 234 97 1 32 263 31 264 1.32e-100 295
MS.gene01625.t1 MTR_8g087720 73.034 178 48 0 5 182 7 184 3.82e-91 268
MS.gene01625.t1 MTR_5g079570 53.012 249 108 3 6 251 12 254 1.01e-88 265
MS.gene01625.t1 MTR_3g008920 52.720 239 110 2 16 254 8 243 2.03e-84 253
MS.gene01625.t1 MTR_4g006730 49.187 246 124 1 15 260 54 298 3.82e-72 224
MS.gene01625.t1 MTR_2g104940 49.206 252 119 3 14 260 56 303 5.05e-72 223
MS.gene01625.t1 MTR_7g010650 48.018 227 116 2 36 262 37 261 8.39e-70 216
MS.gene01625.t1 MTR_5g063930 45.489 266 130 6 1 258 1 259 3.47e-69 214
MS.gene01625.t1 MTR_1g097840 46.693 257 122 5 13 257 47 300 5.35e-66 207
MS.gene01625.t1 MTR_4g006630 50.943 212 103 1 49 260 1 211 4.73e-65 202
MS.gene01625.t1 MTR_4g006730 50.943 212 103 1 49 260 1 211 6.69e-65 202
MS.gene01625.t1 MTR_1g070385 43.192 213 120 1 38 250 64 275 1.27e-49 175
MS.gene01625.t1 MTR_4g006730 43.519 216 109 2 14 229 53 255 5.82e-47 157
MS.gene01625.t1 MTR_1g097840 51.462 171 76 2 32 199 70 236 1.08e-42 147
MS.gene01625.t1 MTR_7g010650 43.750 144 80 1 36 179 37 179 3.85e-36 127
MS.gene01625.t1 MTR_8g013680 33.193 238 148 6 28 254 9 246 8.56e-29 110
MS.gene01625.t1 MTR_1g077540 32.510 243 153 6 19 251 2 243 7.33e-28 108
MS.gene01625.t1 MTR_6g069040 32.129 249 146 6 19 251 2 243 7.71e-28 107
MS.gene01625.t1 MTR_2g101370 34.274 248 145 8 19 254 6 247 7.76e-28 107
MS.gene01625.t1 MTR_1g006490 37.915 211 121 7 35 238 18 225 2.50e-27 106
MS.gene01625.t1 MTR_4g006650 44.828 145 64 3 37 178 5 136 2.33e-25 102
MS.gene01625.t1 MTR_5g012810 35.377 212 128 5 35 238 18 228 8.98e-24 97.1
MS.gene01625.t1 MTR_4g063090 34.529 223 137 5 41 255 26 247 3.72e-23 95.5
MS.gene01625.t1 MTR_1g006590 33.476 233 146 6 35 261 18 247 4.37e-23 95.1
MS.gene01625.t1 MTR_7g103030 34.597 211 130 4 31 234 16 225 6.38e-23 94.7
MS.gene01625.t1 MTR_1g115405 31.624 234 152 4 35 261 20 252 4.35e-20 87.0
MS.gene01625.t1 MTR_7g023350 30.736 231 153 3 35 258 20 250 1.04e-17 80.5
MS.gene01625.t1 MTR_5g010150 32.407 216 124 6 39 235 55 267 1.07e-17 81.3
MS.gene01625.t1 MTR_7g101190 29.615 260 161 7 25 262 28 287 1.83e-17 80.5
MS.gene01625.t1 MTR_5g082070 31.944 216 125 6 39 235 58 270 2.31e-17 80.1
MS.gene01625.t1 MTR_2g094270 32.804 189 114 4 59 235 74 261 2.34e-17 80.1
MS.gene01625.t1 MTR_3g118010 28.634 227 147 4 34 245 35 261 8.12e-17 78.6
MS.gene01625.t1 MTR_3g070210 32.243 214 127 7 39 235 58 270 1.11e-16 78.6
MS.gene01625.t1 MTR_8g098375 31.944 216 125 6 39 235 55 267 2.23e-16 77.4
MS.gene01625.t1 MTR_4g415300 31.132 212 131 6 39 235 59 270 3.56e-16 77.0
MS.gene01625.t1 MTR_2g060360 31.336 217 137 5 35 242 18 231 4.59e-15 73.2
MS.gene01625.t1 MTR_2g094270 31.792 173 108 3 59 222 74 245 4.32e-14 70.9
MS.gene01625.t1 MTR_3g070210 31.633 196 119 6 39 220 58 252 6.98e-14 70.5
MS.gene01625.t1 MTR_1g095070 29.670 182 110 4 79 245 25 203 1.33e-13 68.6
MS.gene01625.t1 MTR_4g059390 32.044 181 105 5 70 235 82 259 2.52e-13 68.9
MS.gene01625.t1 MTR_4g075590 26.457 223 136 8 39 236 40 259 1.06e-11 64.3
MS.gene01625.t1 MTR_1g095070 30.519 154 92 3 79 220 25 175 9.46e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01625.t1 AT4G18910 68.675 249 71 1 22 263 38 286 2.91e-126 361
MS.gene01625.t1 AT4G19030 64.202 257 85 1 14 263 33 289 4.03e-121 348
MS.gene01625.t1 AT5G37820 59.438 249 98 1 20 268 30 275 4.02e-101 296
MS.gene01625.t1 AT5G37820 59.438 249 98 1 20 268 66 311 1.71e-100 296
MS.gene01625.t1 AT5G37810 58.091 241 98 1 23 263 33 270 8.00e-97 286
MS.gene01625.t1 AT1G31885 50.370 270 119 3 1 257 4 271 1.18e-93 279
MS.gene01625.t1 AT4G18910 69.730 185 49 1 22 199 38 222 1.41e-88 263
MS.gene01625.t1 AT2G34390 50.000 286 115 6 1 263 1 281 7.73e-87 260
MS.gene01625.t1 AT1G31885 55.300 217 90 1 48 257 1 217 5.59e-86 258
MS.gene01625.t1 AT4G19030 67.027 185 54 1 22 199 41 225 9.77e-86 255
MS.gene01625.t1 AT2G34390 54.545 231 97 2 37 260 7 236 1.06e-84 253
MS.gene01625.t1 AT4G19030 61.850 173 59 1 14 179 33 205 5.57e-74 225
MS.gene01625.t1 AT1G80760 50.204 245 117 2 8 250 52 293 9.62e-72 223
MS.gene01625.t1 AT4G10380 48.400 250 118 3 18 257 56 304 3.89e-68 213
MS.gene01625.t1 AT2G34390 51.802 222 79 7 1 199 1 217 9.50e-66 204
MS.gene01625.t1 AT2G34390 56.688 157 60 2 32 181 44 199 3.11e-57 183
MS.gene01625.t1 AT3G06100 42.202 218 124 2 35 251 45 261 2.13e-49 164
MS.gene01625.t1 AT3G06100 42.523 214 121 2 39 251 1 213 7.69e-49 161
MS.gene01625.t1 AT3G16240 36.866 217 128 4 27 234 8 224 2.84e-32 119
MS.gene01625.t1 AT1G31885 47.458 118 54 2 1 112 4 119 1.90e-31 114
MS.gene01625.t1 AT2G29870 55.789 95 42 0 151 245 20 114 6.84e-31 112
MS.gene01625.t1 AT5G47450 37.004 227 133 5 22 238 3 229 2.04e-30 114
MS.gene01625.t1 AT3G06100 39.437 142 84 2 112 252 41 181 7.21e-27 103
MS.gene01625.t1 AT1G17810 30.916 262 142 6 22 258 13 260 1.04e-26 105
MS.gene01625.t1 AT4G17340 34.361 227 139 5 22 238 3 229 1.15e-26 104
MS.gene01625.t1 AT1G73190 31.349 252 138 7 22 251 13 251 1.07e-24 99.8
MS.gene01625.t1 AT3G26520 32.780 241 143 6 31 258 17 251 1.04e-23 97.1
MS.gene01625.t1 AT2G36830 31.878 229 146 4 13 234 1 226 1.80e-22 93.6
MS.gene01625.t1 AT2G25810 36.019 211 125 7 35 238 18 225 3.67e-22 92.8
MS.gene01625.t1 AT4G01470 34.199 231 140 6 13 235 1 227 2.97e-21 90.5
MS.gene01625.t1 AT5G60660 28.854 253 152 7 33 261 36 284 2.52e-19 85.9
MS.gene01625.t1 AT4G35100 30.837 227 142 5 34 245 36 262 8.06e-19 84.3
MS.gene01625.t1 AT4G35100 30.837 227 142 5 34 245 36 262 8.06e-19 84.3
MS.gene01625.t1 AT2G16850 29.565 230 141 5 34 245 34 260 1.04e-18 84.0
MS.gene01625.t1 AT2G39010 32.292 192 118 4 66 245 77 268 6.55e-18 82.0
MS.gene01625.t1 AT1G01620 32.093 215 125 5 39 235 55 266 2.05e-17 80.5
MS.gene01625.t1 AT3G61430 31.628 215 126 5 39 235 55 266 2.55e-17 80.1
MS.gene01625.t1 AT3G61430 31.628 215 126 5 39 235 55 266 2.55e-17 80.1
MS.gene01625.t1 AT1G01620 33.684 190 108 4 61 235 8 194 2.95e-17 78.6
MS.gene01625.t1 AT4G00430 32.093 215 125 5 39 235 56 267 3.58e-17 79.7
MS.gene01625.t1 AT3G47440 30.045 223 132 6 35 242 22 235 3.61e-17 79.3
MS.gene01625.t1 AT3G53420 31.414 191 113 4 70 245 82 269 9.20e-17 78.6
MS.gene01625.t1 AT3G53420 31.414 191 113 4 70 245 82 269 9.20e-17 78.6
MS.gene01625.t1 AT4G23400 31.628 215 126 5 39 235 56 267 1.40e-16 78.2
MS.gene01625.t1 AT2G45960 31.163 215 127 5 39 235 55 266 7.12e-16 76.3
MS.gene01625.t1 AT2G37170 27.966 236 143 6 34 245 35 267 8.74e-16 75.9
MS.gene01625.t1 AT3G54820 27.897 233 147 5 34 245 36 268 9.29e-16 75.9
MS.gene01625.t1 AT2G37170 27.966 236 143 6 34 245 78 310 1.43e-15 75.9
MS.gene01625.t1 AT2G37180 27.966 236 143 6 34 245 35 267 4.79e-15 73.9
MS.gene01625.t1 AT2G45960 31.472 197 117 4 39 220 55 248 1.28e-13 69.7
MS.gene01625.t1 AT2G45960 31.472 197 117 4 39 220 55 248 1.49e-13 69.7

Find 73 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTTATGTTCATCATTTC+TGG 0.019796 2.2:-66774858 MS.gene01625:CDS
TCCTGGAATTTCTATTGTTT+GGG 0.178041 2.2:-66775828 MS.gene01625:CDS
TTCCTGGAATTTCTATTGTT+TGG 0.214894 2.2:-66775829 MS.gene01625:CDS
CCTTGCTTCCTGCACCTTTG+AGG 0.279429 2.2:+66774113 None:intergenic
ACATTCTACTTGATATTTGC+TGG 0.321198 2.2:-66775899 MS.gene01625:CDS
TGTTTGGGGACTTGCTGTTA+TGG 0.331507 2.2:-66775813 MS.gene01625:CDS
GTCACCTATTCTAGGAGCTT+TGG 0.344731 2.2:-66774209 MS.gene01625:CDS
GATAAAGTAGTGACACTTCC+TGG 0.350687 2.2:-66775845 MS.gene01625:CDS
TCCATATTCCTCTATATTCA+TGG 0.360944 2.2:+66774242 None:intergenic
ATTGCTTTAGATTGGTGAAT+TGG 0.362837 2.2:-66774524 MS.gene01625:intron
GCTGGTCCTAAGCTTCTTGC+TGG 0.364030 2.2:+66774274 None:intergenic
GGCAAGGAAAATCAATTTGC+AGG 0.368386 2.2:-66774939 MS.gene01625:CDS
ATACTAAATGTGTTGTTTGC+GGG 0.374820 2.2:-66774475 MS.gene01625:CDS
TATGTTGTGTCACCTATTCT+AGG 0.387825 2.2:-66774217 MS.gene01625:CDS
TCACTACTTTATCATTGTTA+AGG 0.387999 2.2:+66775855 None:intergenic
ATGGTATTAGTATATTCTAT+TGG 0.391418 2.2:-66775794 MS.gene01625:CDS
TGGTGACAGCGGGATTGAAA+TGG 0.397322 2.2:+66775756 None:intergenic
TGGTGATTTACCTGCTTCAA+TGG 0.403736 2.2:+66775707 None:intergenic
CTGCTTCAATGGAAACCTTC+CGG 0.415943 2.2:+66775718 None:intergenic
TACTTGATATTTGCTGGATG+TGG 0.422703 2.2:-66775893 MS.gene01625:CDS
CTCCAGAAATGATGAACATA+AGG 0.423335 2.2:+66774856 None:intergenic
CTTTAGATTGGTGAATTGGC+TGG 0.424124 2.2:-66774520 MS.gene01625:intron
TATTCTATTGGCCATATCTC+TGG 0.439778 2.2:-66775782 MS.gene01625:CDS
CCCCAAACAATAGAAATTCC+AGG 0.443536 2.2:+66775827 None:intergenic
TGAAATGGGCACCAGAGATA+TGG 0.444610 2.2:+66775771 None:intergenic
CCAGCCAAAGCTCCTAGAAT+AGG 0.460240 2.2:+66774205 None:intergenic
TAGGAGCTTTGGCTGGTACA+TGG 0.462758 2.2:-66774198 MS.gene01625:CDS
GCTCTCAAACTTATATTCAA+TGG 0.464325 2.2:-66774960 MS.gene01625:CDS
ATTCATTGATGCTCCTGTGA+TGG 0.469127 2.2:+66774297 None:intergenic
TTTGAGGAATGAAGAACTCT+TGG 0.469564 2.2:+66774129 None:intergenic
TATACTAAATGTGTTGTTTG+CGG 0.470231 2.2:-66774476 MS.gene01625:CDS
TTCATTGATGCTCCTGTGAT+GGG 0.472526 2.2:+66774298 None:intergenic
ATGAATCCAGCAAGAAGCTT+AGG 0.484395 2.2:-66774280 MS.gene01625:CDS
TATATAATTGCTCAGGTCGT+TGG 0.487350 2.2:-66775002 MS.gene01625:CDS
TATTCATGGTGCACAATAGC+TGG 0.496895 2.2:+66774256 None:intergenic
GGTGACAGCGGGATTGAAAT+GGG 0.499640 2.2:+66775757 None:intergenic
CGGAAGGTTTCCATTGAAGC+AGG 0.504912 2.2:-66775717 MS.gene01625:intron
TTTGCAGGAACACTTCCTGC+TGG 0.505103 2.2:-66774924 MS.gene01625:CDS
TCTTCATTCCTCAAAGGTGC+AGG 0.507670 2.2:-66774121 MS.gene01625:CDS
AATATAGTTGGTAGCAGAAG+TGG 0.514295 2.2:-66775927 MS.gene01625:intron
GAATTGGCTGGACTTGCAGT+TGG 0.518324 2.2:-66774508 MS.gene01625:CDS
ACCTGAGCAATTATATAAGC+TGG 0.518659 2.2:+66775008 None:intergenic
ACCAGCTTATATAATTGCTC+AGG 0.519565 2.2:-66775009 MS.gene01625:CDS
CCTGGAATTTCTATTGTTTG+GGG 0.522115 2.2:-66775827 MS.gene01625:CDS
CCTATTCTAGGAGCTTTGGC+TGG 0.527244 2.2:-66774205 MS.gene01625:CDS
CTCTTGGTGAGCTCACGTAC+TGG 0.527633 2.2:+66774145 None:intergenic
ACCATGAATATAGAGGAATA+TGG 0.529709 2.2:-66774243 MS.gene01625:CDS
CCTCAAAGGTGCAGGAAGCA+AGG 0.539036 2.2:-66774113 MS.gene01625:CDS
CTCAAAGGTGCAGGAAGCAA+GGG 0.548913 2.2:-66774112 MS.gene01625:CDS
TACTAAATGTGTTGTTTGCG+GGG 0.554404 2.2:-66774474 MS.gene01625:intron
GTGTGAGCAATGGTGACAGC+GGG 0.558512 2.2:+66775746 None:intergenic
AACATGGGTGATATTAGCAA+TGG 0.558983 2.2:-66776136 MS.gene01625:CDS
ACCATTGCTCACACTACCAC+CGG 0.562160 2.2:-66775737 MS.gene01625:CDS
CTGGCTTATTAGTGATTCTG+AGG 0.564075 2.2:+66774164 None:intergenic
TGCTGGATGTGGTTCTGTGG+TGG 0.564205 2.2:-66775882 MS.gene01625:CDS
ATAGTTGGTAGCAGAAGTGG+TGG 0.567543 2.2:-66775924 MS.gene01625:intron
ATTTGCTGGATGTGGTTCTG+TGG 0.593346 2.2:-66775885 MS.gene01625:CDS
GCTTGAAGATCAGAACCAGC+AGG 0.594492 2.2:+66774909 None:intergenic
CAAACTTATATTCAATGGCA+AGG 0.602566 2.2:-66774955 MS.gene01625:CDS
AAGAGTTCTTCATTCCTCAA+AGG 0.603468 2.2:-66774127 MS.gene01625:CDS
AGTGATTCTGAGGAAAGTGT+AGG 0.628271 2.2:+66774174 None:intergenic
ATATTTGACAGACCCATCAC+AGG 0.629986 2.2:-66774310 MS.gene01625:intron
GATTACCGCTCTATTATCAG+TGG 0.633724 2.2:+66774830 None:intergenic
GTTGGCTCAACACTTGCAAG+TGG 0.642506 2.2:-66774984 MS.gene01625:CDS
ATTGTGCACCATGAATATAG+AGG 0.654237 2.2:-66774250 MS.gene01625:CDS
TAGCAATGGAAATCTTGATG+TGG 0.656350 2.2:-66776122 MS.gene01625:CDS
TTGCTCACACTACCACCGGA+AGG 0.656735 2.2:-66775733 MS.gene01625:CDS
TCCGGTGGTAGTGTGAGCAA+TGG 0.668876 2.2:+66775736 None:intergenic
CTTCAATGGAAACCTTCCGG+TGG 0.695539 2.2:+66775721 None:intergenic
TAGTTGGTAGCAGAAGTGGT+GGG 0.737512 2.2:-66775923 MS.gene01625:CDS
AGTGTGAGCAATGGTGACAG+CGG 0.739883 2.2:+66775745 None:intergenic
AGTTGCCACTGATAATAGAG+CGG 0.742875 2.2:-66774835 MS.gene01625:intron
GTGGTTATGAATGTAAACGA+TGG 0.756470 2.2:-66776103 MS.gene01625:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAAAAAAAAAAATGCT+TGG - chr2.2:66775431-66775450 MS.gene01625:intron 10.0%
!!! AAAAAATTAATTGTTGTTTA+TGG - chr2.2:66774231-66774250 MS.gene01625:CDS 10.0%
!!! ATAAACAACAATTAATTTTT+TGG + chr2.2:66774232-66774251 None:intergenic 10.0%
!! AGGTTTAAATAAAATTTAAC+TGG - chr2.2:66775891-66775910 MS.gene01625:CDS 15.0%
!! TAGATGTATAAATATATTCT+AGG - chr2.2:66775599-66775618 MS.gene01625:intron 15.0%
!! TTAAATATAATGTGTAATCT+TGG + chr2.2:66775839-66775858 None:intergenic 15.0%
!!! AGAAAAAGTCAATTTTATTT+CGG + chr2.2:66775551-66775570 None:intergenic 15.0%
!!! TATTATTTATTGCTTTAGAT+TGG - chr2.2:66775703-66775722 MS.gene01625:intron 15.0%
!! AAAAATTCAAAACTCTTTAG+AGG + chr2.2:66774681-66774700 None:intergenic 20.0%
!! ATGGTATTAGTATATTCTAT+TGG - chr2.2:66774441-66774460 MS.gene01625:intron 20.0%
!! GCACTTTATGATATAATATT+CGG + chr2.2:66775806-66775825 None:intergenic 20.0%
!! TATACAAAAACTATTACACA+AGG + chr2.2:66775679-66775698 None:intergenic 20.0%
!! TCTTATATAGTTGAGAAAAT+AGG - chr2.2:66774740-66774759 MS.gene01625:intron 20.0%
!! TTGTCTGAATATGAATTATA+GGG + chr2.2:66774563-66774582 None:intergenic 20.0%
!! TTTGTCTGAATATGAATTAT+AGG + chr2.2:66774564-66774583 None:intergenic 20.0%
!!! AAAAATGTTGTTTTCGTTAT+AGG + chr2.2:66775165-66775184 None:intergenic 20.0%
!!! ATAACGAAAACAACATTTTT+AGG - chr2.2:66775165-66775184 MS.gene01625:intron 20.0%
!!! CTAAAGAGTTTTGAATTTTT+GGG - chr2.2:66774681-66774700 MS.gene01625:intron 20.0%
!!! TAAAGAGTTTTGAATTTTTG+GGG - chr2.2:66774682-66774701 MS.gene01625:intron 20.0%
!!! TCTAAAGAGTTTTGAATTTT+TGG - chr2.2:66774680-66774699 MS.gene01625:intron 20.0%
! ATCTCGTTAAGTTATTTGAT+CGG - chr2.2:66774599-66774618 MS.gene01625:intron 25.0%
! CTTTGATTGTTGAATATAGT+TGG - chr2.2:66774296-66774315 MS.gene01625:CDS 25.0%
! GATGTTCTGACTAAAAAAAA+TGG - chr2.2:66774265-66774284 MS.gene01625:CDS 25.0%
! TATGTGAAGAAGAAAATAGT+TGG - chr2.2:66774947-66774966 MS.gene01625:CDS 25.0%
! TCACTACTTTATCATTGTTA+AGG + chr2.2:66774383-66774402 None:intergenic 25.0%
!! AACTATTTTCTTCTTCACAT+AGG + chr2.2:66774948-66774967 None:intergenic 25.0%
!!! TATACTAAATGTGTTGTTTG+CGG - chr2.2:66775759-66775778 MS.gene01625:CDS 25.0%
!!! TGAACTTAATTTTTTCTTGC+AGG - chr2.2:66775202-66775221 MS.gene01625:intron 25.0%
ACATTCTACTTGATATTTGC+TGG - chr2.2:66774336-66774355 MS.gene01625:intron 30.0%
ACCATGAATATAGAGGAATA+TGG - chr2.2:66775992-66776011 MS.gene01625:intron 30.0%
AGAGATAGTGACTTAAATTG+TGG + chr2.2:66775056-66775075 None:intergenic 30.0%
ATAGTTGGAAGACCATTATT+TGG - chr2.2:66774962-66774981 MS.gene01625:CDS 30.0%
CAAACTTATATTCAATGGCA+AGG - chr2.2:66775280-66775299 MS.gene01625:intron 30.0%
GCTCTCAAACTTATATTCAA+TGG - chr2.2:66775275-66775294 MS.gene01625:intron 30.0%
GTGACATGTTATTATGAGAA+AGG - chr2.2:66775871-66775890 MS.gene01625:CDS 30.0%
TAGTTGGAAGACCATTATTT+GGG - chr2.2:66774963-66774982 MS.gene01625:CDS 30.0%
TCCATATTCCTCTATATTCA+TGG + chr2.2:66775996-66776015 None:intergenic 30.0%
TCCTGGAATTTCTATTGTTT+GGG - chr2.2:66774407-66774426 MS.gene01625:intron 30.0%
TGTTGACAATTATAGACGAT+GGG - chr2.2:66774792-66774811 MS.gene01625:intron 30.0%
TTCCTGGAATTTCTATTGTT+TGG - chr2.2:66774406-66774425 MS.gene01625:intron 30.0%
TTCCTTATGTTCATCATTTC+TGG - chr2.2:66775377-66775396 MS.gene01625:intron 30.0%
TTGTTGACAATTATAGACGA+TGG - chr2.2:66774791-66774810 MS.gene01625:intron 30.0%
! ATTGCTTTAGATTGGTGAAT+TGG - chr2.2:66775711-66775730 MS.gene01625:intron 30.0%
!! AAGTCAATTTTATTTCGGCA+AGG + chr2.2:66775546-66775565 None:intergenic 30.0%
!! ATACTAAATGTGTTGTTTGC+GGG - chr2.2:66775760-66775779 MS.gene01625:CDS 30.0%
AAATAGGATCCTCAGAGTAA+TGG - chr2.2:66774756-66774775 MS.gene01625:intron 35.0%
AAGAGTTCTTCATTCCTCAA+AGG - chr2.2:66776108-66776127 MS.gene01625:CDS 35.0%
ACCAGCTTATATAATTGCTC+AGG - chr2.2:66775226-66775245 MS.gene01625:intron 35.0%
ACCTGAGCAATTATATAAGC+TGG + chr2.2:66775230-66775249 None:intergenic 35.0%
AGAGAGATAGACACATATTG+TGG - chr2.2:66775102-66775121 MS.gene01625:intron 35.0%
AGATAGACACATATTGTGGA+AGG - chr2.2:66775106-66775125 MS.gene01625:intron 35.0%
ATTGTGCACCATGAATATAG+AGG - chr2.2:66775985-66776004 MS.gene01625:intron 35.0%
CAATGTAGTAGCAAAATGGA+AGG - chr2.2:66774891-66774910 MS.gene01625:CDS 35.0%
CCTGGAATTTCTATTGTTTG+GGG - chr2.2:66774408-66774427 MS.gene01625:intron 35.0%
CTCCAGAAATGATGAACATA+AGG + chr2.2:66775382-66775401 None:intergenic 35.0%
CTTAAATTGTGGATGAGAGA+AGG + chr2.2:66775045-66775064 None:intergenic 35.0%
GTGGTTATGAATGTAAACGA+TGG - chr2.2:66774132-66774151 MS.gene01625:CDS 35.0%
TAGCAATGGAAATCTTGATG+TGG - chr2.2:66774113-66774132 MS.gene01625:CDS 35.0%
TATATAATTGCTCAGGTCGT+TGG - chr2.2:66775233-66775252 MS.gene01625:intron 35.0%
TATGTTGTGTCACCTATTCT+AGG - chr2.2:66776018-66776037 MS.gene01625:intron 35.0%
TATTCTATTGGCCATATCTC+TGG - chr2.2:66774453-66774472 MS.gene01625:intron 35.0%
TCAACATAAGCTTCGAACAT+AGG + chr2.2:66774989-66775008 None:intergenic 35.0%
TCGTTAAGTTATTTGATCGG+AGG - chr2.2:66774602-66774621 MS.gene01625:intron 35.0%
TGCTCAATGTAGTAGCAAAA+TGG - chr2.2:66774887-66774906 MS.gene01625:CDS 35.0%
TGGAAGAAGATGATCCAATT+CGG + chr2.2:66775012-66775031 None:intergenic 35.0%
TTTGAGGAATGAAGAACTCT+TGG + chr2.2:66776109-66776128 None:intergenic 35.0%
! TACTTGATATTTGCTGGATG+TGG - chr2.2:66774342-66774361 MS.gene01625:intron 35.0%
! TCATGTTCCTCTTTTGCAAA+AGG - chr2.2:66774191-66774210 MS.gene01625:CDS 35.0%
! TTCTGATCTTCAAGCTTTTG+TGG - chr2.2:66775334-66775353 MS.gene01625:intron 35.0%
!! AATATAGTTGGTAGCAGAAG+TGG - chr2.2:66774308-66774327 MS.gene01625:intron 35.0%
!! GAATTATAGGGCATTGTGAT+TGG + chr2.2:66774551-66774570 None:intergenic 35.0%
!! TACTAAATGTGTTGTTTGCG+GGG - chr2.2:66775761-66775780 MS.gene01625:CDS 35.0%
AGACACATATTGTGGAAGGA+GGG - chr2.2:66775110-66775129 MS.gene01625:intron 40.0%
AGTTGCCACTGATAATAGAG+CGG - chr2.2:66775400-66775419 MS.gene01625:intron 40.0%
ATATTTGACAGACCCATCAC+AGG - chr2.2:66775925-66775944 MS.gene01625:intron 40.0%
ATCGGAGGTGATTAGAAGAA+CGG - chr2.2:66774617-66774636 MS.gene01625:intron 40.0%
ATGAATCCAGCAAGAAGCTT+AGG - chr2.2:66775955-66775974 MS.gene01625:intron 40.0%
ATTCATTGATGCTCCTGTGA+TGG + chr2.2:66775941-66775960 None:intergenic 40.0%
CATTGCATCTTGAATGCATG+CGG - chr2.2:66774830-66774849 MS.gene01625:intron 40.0%
CCCCAAACAATAGAAATTCC+AGG + chr2.2:66774411-66774430 None:intergenic 40.0%
CTGGCTTATTAGTGATTCTG+AGG + chr2.2:66776074-66776093 None:intergenic 40.0%
CTTTAGATTGGTGAATTGGC+TGG - chr2.2:66775715-66775734 MS.gene01625:intron 40.0%
GAAGCTTATGTTGACCGAAT+TGG - chr2.2:66774995-66775014 MS.gene01625:CDS 40.0%
GAGCTTGTAGGTTGTTGAAA+CGG - chr2.2:66774648-66774667 MS.gene01625:intron 40.0%
GATAAAGTAGTGACACTTCC+TGG - chr2.2:66774390-66774409 MS.gene01625:intron 40.0%
GATTACCGCTCTATTATCAG+TGG + chr2.2:66775408-66775427 None:intergenic 40.0%
GGCAAGGAAAATCAATTTGC+AGG - chr2.2:66775296-66775315 MS.gene01625:intron 40.0%
TAGACACATATTGTGGAAGG+AGG - chr2.2:66775109-66775128 MS.gene01625:intron 40.0%
TATTCATGGTGCACAATAGC+TGG + chr2.2:66775982-66776001 None:intergenic 40.0%
TCGAACATAGGCCCAAATAA+TGG + chr2.2:66774977-66774996 None:intergenic 40.0%
TGGTGATTTACCTGCTTCAA+TGG + chr2.2:66774531-66774550 None:intergenic 40.0%
TTCATTGATGCTCCTGTGAT+GGG + chr2.2:66775940-66775959 None:intergenic 40.0%
! AATGGAAGGTGTGAAGAGTA+TGG - chr2.2:66774905-66774924 MS.gene01625:CDS 40.0%
! AGTGATTCTGAGGAAAGTGT+AGG + chr2.2:66776064-66776083 None:intergenic 40.0%
! ATGGAAGGTGTGAAGAGTAT+GGG - chr2.2:66774906-66774925 MS.gene01625:CDS 40.0%
! CAAGTCACCTTTTGCAAAAG+AGG + chr2.2:66774201-66774220 None:intergenic 40.0%
AGGATCCTCAGAGTAATGGT+TGG - chr2.2:66774760-66774779 MS.gene01625:intron 45.0%
ATTGTGGATGAGAGAAGGTG+AGG + chr2.2:66775040-66775059 None:intergenic 45.0%
CTGCTTCAATGGAAACCTTC+CGG + chr2.2:66774520-66774539 None:intergenic 45.0%
CTTGTAGGTTGTTGAAACGG+CGG - chr2.2:66774651-66774670 MS.gene01625:intron 45.0%
GTTAGAAGATTGCACCTTGG+AGG - chr2.2:66774704-66774723 MS.gene01625:intron 45.0%
TGAAATGGGCACCAGAGATA+TGG + chr2.2:66774467-66774486 None:intergenic 45.0%
TTGTAGGTTGTTGAAACGGC+GGG - chr2.2:66774652-66774671 MS.gene01625:intron 45.0%
TTGTGGATGAGAGAAGGTGA+GGG + chr2.2:66775039-66775058 None:intergenic 45.0%
! ATTTGCTGGATGTGGTTCTG+TGG - chr2.2:66774350-66774369 MS.gene01625:intron 45.0%
! GTCACCTATTCTAGGAGCTT+TGG - chr2.2:66776026-66776045 MS.gene01625:intron 45.0%
! TCTTCATTCCTCAAAGGTGC+AGG - chr2.2:66776114-66776133 MS.gene01625:CDS 45.0%
!! AGGAGGCATTACAGACTTAC+GGG - chr2.2:66774853-66774872 MS.gene01625:CDS 45.0%
!! ATAGTTGGTAGCAGAAGTGG+TGG - chr2.2:66774311-66774330 MS.gene01625:intron 45.0%
!! GAGGAGGCATTACAGACTTA+CGG - chr2.2:66774852-66774871 MS.gene01625:CDS 45.0%
!! TAGTTGGTAGCAGAAGTGGT+GGG - chr2.2:66774312-66774331 MS.gene01625:intron 45.0%
!! TGTTTGGGGACTTGCTGTTA+TGG - chr2.2:66774422-66774441 MS.gene01625:intron 45.0%
AAGGAGGGAGAAAGAGAGAG+TGG - chr2.2:66775125-66775144 MS.gene01625:intron 50.0%
ACCATTGCTCACACTACCAC+CGG - chr2.2:66774498-66774517 MS.gene01625:CDS 50.0%
ACGGCACTTGATGAGCTTGT+AGG - chr2.2:66774636-66774655 MS.gene01625:intron 50.0%
AGATTGCACCTTGGAGGACA+TGG - chr2.2:66774710-66774729 MS.gene01625:intron 50.0%
AGTACATCCCATGTCCTCCA+AGG + chr2.2:66774721-66774740 None:intergenic 50.0%
AGTGTGAGCAATGGTGACAG+CGG + chr2.2:66774493-66774512 None:intergenic 50.0%
ATCTTGAATGCATGCGGAGG+AGG - chr2.2:66774836-66774855 MS.gene01625:CDS 50.0%
CCAGCCAAAGCTCCTAGAAT+AGG + chr2.2:66776033-66776052 None:intergenic 50.0%
CGGAAGGTTTCCATTGAAGC+AGG - chr2.2:66774518-66774537 MS.gene01625:intron 50.0%
CTTCAATGGAAACCTTCCGG+TGG + chr2.2:66774517-66774536 None:intergenic 50.0%
GAATTGGCTGGACTTGCAGT+TGG - chr2.2:66775727-66775746 MS.gene01625:CDS 50.0%
GATTGCACCTTGGAGGACAT+GGG - chr2.2:66774711-66774730 MS.gene01625:intron 50.0%
GCTCTCCAACCATTACTCTG+AGG + chr2.2:66774768-66774787 None:intergenic 50.0%
GCTTGAAGATCAGAACCAGC+AGG + chr2.2:66775329-66775348 None:intergenic 50.0%
GGGGTTAGAAGATTGCACCT+TGG - chr2.2:66774701-66774720 MS.gene01625:intron 50.0%
GGTGACAGCGGGATTGAAAT+GGG + chr2.2:66774481-66774500 None:intergenic 50.0%
TGCATCTTGAATGCATGCGG+AGG - chr2.2:66774833-66774852 MS.gene01625:intron 50.0%
TGGTGACAGCGGGATTGAAA+TGG + chr2.2:66774482-66774501 None:intergenic 50.0%
TGTAGGTTGTTGAAACGGCG+GGG - chr2.2:66774653-66774672 MS.gene01625:intron 50.0%
TTTGCAGGAACACTTCCTGC+TGG - chr2.2:66775311-66775330 MS.gene01625:intron 50.0%
! CCTATTCTAGGAGCTTTGGC+TGG - chr2.2:66776030-66776049 MS.gene01625:intron 50.0%
! CTCAAAGGTGCAGGAAGCAA+GGG - chr2.2:66776123-66776142 MS.gene01625:CDS 50.0%
! GTTGGCTCAACACTTGCAAG+TGG - chr2.2:66775251-66775270 MS.gene01625:intron 50.0%
! TAGGAGCTTTGGCTGGTACA+TGG - chr2.2:66776037-66776056 MS.gene01625:intron 50.0%
CCTTGCTTCCTGCACCTTTG+AGG + chr2.2:66776125-66776144 None:intergenic 55.0%
CTCTTGGTGAGCTCACGTAC+TGG + chr2.2:66776093-66776112 None:intergenic 55.0%
GCTGGTCCTAAGCTTCTTGC+TGG + chr2.2:66775964-66775983 None:intergenic 55.0%
GTGTGAGCAATGGTGACAGC+GGG + chr2.2:66774492-66774511 None:intergenic 55.0%
TGAGAGAAGGTGAGGGTGAG+TGG + chr2.2:66775032-66775051 None:intergenic 55.0%
TTGCTCACACTACCACCGGA+AGG - chr2.2:66774502-66774521 MS.gene01625:CDS 55.0%
! CCTCAAAGGTGCAGGAAGCA+AGG - chr2.2:66776122-66776141 MS.gene01625:CDS 55.0%
! TCCGGTGGTAGTGTGAGCAA+TGG + chr2.2:66774502-66774521 None:intergenic 55.0%
! TGCTGGATGTGGTTCTGTGG+TGG - chr2.2:66774353-66774372 MS.gene01625:intron 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 66774102 66776155 66774102 ID=MS.gene01625
chr2.2 mRNA 66774102 66776155 66774102 ID=MS.gene01625.t1;Parent=MS.gene01625
chr2.2 exon 66776045 66776155 66776045 ID=MS.gene01625.t1.exon1;Parent=MS.gene01625.t1
chr2.2 CDS 66776045 66776155 66776045 ID=cds.MS.gene01625.t1;Parent=MS.gene01625.t1
chr2.2 exon 66775718 66775942 66775718 ID=MS.gene01625.t1.exon2;Parent=MS.gene01625.t1
chr2.2 CDS 66775718 66775942 66775718 ID=cds.MS.gene01625.t1;Parent=MS.gene01625.t1
chr2.2 exon 66774836 66775033 66774836 ID=MS.gene01625.t1.exon3;Parent=MS.gene01625.t1
chr2.2 CDS 66774836 66775033 66774836 ID=cds.MS.gene01625.t1;Parent=MS.gene01625.t1
chr2.2 exon 66774475 66774536 66774475 ID=MS.gene01625.t1.exon4;Parent=MS.gene01625.t1
chr2.2 CDS 66774475 66774536 66774475 ID=cds.MS.gene01625.t1;Parent=MS.gene01625.t1
chr2.2 exon 66774102 66774321 66774102 ID=MS.gene01625.t1.exon5;Parent=MS.gene01625.t1
chr2.2 CDS 66774102 66774321 66774102 ID=cds.MS.gene01625.t1;Parent=MS.gene01625.t1
Gene Sequence

>MS.gene01625

ATGGGTGATATTAGCAATGGAAATCTTGATGTGGTTATGAATGTAAACGATGGTGCAACAAAAAAATGTGACGACACAAGCATTGATGATCATGTTCCTCTTTTGCAAAAGGTGACTTGTTTATCTTCCAAAAAATTAATTGTTGTTTATGGTTAATTAATTAGATGTTCTGACTAAAAAAAATGGATGTTTTGCTTTGATTGTTGAATATAGTTGGTAGCAGAAGTGGTGGGAACATTCTACTTGATATTTGCTGGATGTGGTTCTGTGGTGGTGAACCTTAACAATGATAAAGTAGTGACACTTCCTGGAATTTCTATTGTTTGGGGACTTGCTGTTATGGTATTAGTATATTCTATTGGCCATATCTCTGGTGCCCATTTCAATCCCGCTGTCACCATTGCTCACACTACCACCGGAAGGTTTCCATTGAAGCAGGTAAATCACCAATCACAATGCCCTATAATTCATATTCAGACAAACATAATTTTCACAGCATCTCGTTAAGTTATTTGATCGGAGGTGATTAGAAGAACGGCACTTGATGAGCTTGTAGGTTGTTGAAACGGCGGGGTACCTCTAAAGAGTTTTGAATTTTTGGGGTTAGAAGATTGCACCTTGGAGGACATGGGATGTACTCTTATATAGTTGAGAAAATAGGATCCTCAGAGTAATGGTTGGAGAGCTGTTTGTTGACAATTATAGACGATGGGTCTGTAACAGTCGAACATTGCATCTTGAATGCATGCGGAGGAGGCATTACAGACTTACGGGATCTAAACAATTGCTCAATGTAGTAGCAAAATGGAAGGTGTGAAGAGTATGGGCGAAAGCGAGTTTGAGCCTATGTGAAGAAGAAAATAGTTGGAAGACCATTATTTGGGCCTATGTTCGAAGCTTATGTTGACCGAATTGGATCATCTTCTTCCACTCACCCTCACCTTCTCTCATCCACAATTTAAGTCACTATCTCTCTCTTGATATTTTATCTAAATTTTAGAGAGAGATAGACACATATTGTGGAAGGAGGGAGAAAGAGAGAGTGGATGAAATGAGAGATCCTATAACGAAAACAACATTTTTAGGAACATTATTAACTTTGAACTTAATTTTTTCTTGCAGGTACCAGCTTATATAATTGCTCAGGTCGTTGGCTCAACACTTGCAAGTGGAGCTCTCAAACTTATATTCAATGGCAAGGAAAATCAATTTGCAGGAACACTTCCTGCTGGTTCTGATCTTCAAGCTTTTGTGGTTGAATTTATTATCACTTTCTTCCTTATGTTCATCATTTCTGGAGTTGCCACTGATAATAGAGCGGTAATCTTTTTAAAAAAAAAAAAAATGCTTGGTTCATAATATTAATTGATACTTATATTTTTATTAAACATTTTGATGAAGATTTAAGATATGCTAATAATTTATATGAGATGAAAAATCACCTTGCCGAAATAAAATTGACTTTTTCTTTCAAAAACAATAAAAAAGTTCATTGATTAGATGTATAAATATATTCTAGGCTTATATAAATTTTTTTTTTTGAGAGATTCTATGCTTATATAAATATTATTTAACCTTGTGTAATAGTTTTTGTATATTCATATTATTTATTGCTTTAGATTGGTGAATTGGCTGGACTTGCAGTTGGATCTACAGTTATACTAAATGTGTTGTTTGCGGGGTATTGATCTTAAAACTTTACCGAATATTATATCATAAAGTGCATATTTAGCACCAAGATTACACATTATATTTAATAAAATTAATATGTGACATGTTATTATGAGAAAGGTTTAAATAAAATTTAACTGGTGTGCGAACATATATTTGACAGACCCATCACAGGAGCATCAATGAATCCAGCAAGAAGCTTAGGACCAGCTATTGTGCACCATGAATATAGAGGAATATGGATATATGTTGTGTCACCTATTCTAGGAGCTTTGGCTGGTACATGGACCTACACTTTCCTCAGAATCACTAATAAGCCAGTACGTGAGCTCACCAAGAGTTCTTCATTCCTCAAAGGTGCAGGAAGCAAGGGAGCTGAGTGA
Protein sequence

>MS.gene01625.t1

MGDISNGNLDVVMNVNDGATKKCDDTSIDDHVPLLQKLVAEVVGTFYLIFAGCGSVVVNLNNDKVVTLPGISIVWGLAVMVLVYSIGHISGAHFNPAVTIAHTTTGRFPLKQVPAYIIAQVVGSTLASGALKLIFNGKENQFAGTLPAGSDLQAFVVEFIITFFLMFIISGVATDNRAIGELAGLAVGSTVILNVLFAGPITGASMNPARSLGPAIVHHEYRGIWIYVVSPILGALAGTWTYTFLRITNKPVRELTKSSSFLKGAGSKGAE