AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065972

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065972.t1 MTR_8g087710 97.826 276 6 0 1 276 1 276 0.0 549
MS.gene065972.t1 MTR_8g087720 73.208 265 71 0 4 268 3 267 1.98e-150 421
MS.gene065972.t1 MTR_2g017570 71.218 271 78 0 4 274 3 273 1.71e-144 406
MS.gene065972.t1 MTR_2g017610 71.218 271 78 0 4 274 3 273 1.48e-143 404
MS.gene065972.t1 MTR_2g017590 68.340 259 82 0 13 271 10 268 2.25e-135 383
MS.gene065972.t1 MTR_2g017660 64.706 272 89 2 4 268 3 274 5.96e-131 372
MS.gene065972.t1 MTR_2g017620 65.789 266 91 0 4 269 3 268 9.97e-129 366
MS.gene065972.t1 MTR_2g017620 67.323 254 83 0 16 269 1 254 3.35e-127 362
MS.gene065972.t1 MTR_4g084880 53.875 271 119 2 9 273 1 271 3.66e-106 309
MS.gene065972.t1 MTR_8g087720 72.362 199 55 0 4 202 3 201 1.29e-105 305
MS.gene065972.t1 MTR_2g017620 70.588 187 55 0 83 269 1 187 4.42e-95 278
MS.gene065972.t1 MTR_8g087720 67.005 197 64 1 4 200 3 198 1.58e-92 272
MS.gene065972.t1 MTR_5g079570 50.775 258 119 4 1 254 1 254 3.04e-85 256
MS.gene065972.t1 MTR_3g008920 55.605 223 98 1 35 257 22 243 3.67e-83 250
MS.gene065972.t1 MTR_4g006730 53.680 231 102 2 35 263 71 298 5.65e-78 239
MS.gene065972.t1 MTR_2g104940 52.863 227 102 2 39 263 80 303 5.06e-76 234
MS.gene065972.t1 MTR_7g010650 48.148 216 111 1 37 252 35 249 1.04e-72 224
MS.gene065972.t1 MTR_4g006730 53.738 214 94 2 52 263 1 211 4.23e-72 221
MS.gene065972.t1 MTR_4g006630 53.738 214 94 2 52 263 1 211 5.26e-72 220
MS.gene065972.t1 MTR_1g097840 49.362 235 108 3 34 260 69 300 2.75e-69 216
MS.gene065972.t1 MTR_5g063930 46.067 267 118 8 4 261 10 259 3.75e-66 207
MS.gene065972.t1 MTR_4g006730 48.259 201 87 3 35 233 71 256 4.30e-51 168
MS.gene065972.t1 MTR_1g097840 51.462 171 78 2 34 202 69 236 1.39e-46 157
MS.gene065972.t1 MTR_1g070385 40.271 221 131 1 33 253 56 275 2.50e-46 166
MS.gene065972.t1 MTR_7g010650 45.890 146 78 1 37 182 35 179 3.37e-40 138
MS.gene065972.t1 MTR_4g006650 50.694 144 57 3 40 181 5 136 2.72e-32 121
MS.gene065972.t1 MTR_8g013680 33.028 218 139 3 38 248 18 235 1.58e-26 104
MS.gene065972.t1 MTR_2g101370 32.645 242 145 7 34 264 15 249 3.13e-25 100
MS.gene065972.t1 MTR_5g012810 33.333 246 144 6 28 257 5 246 5.00e-25 100
MS.gene065972.t1 MTR_1g006490 31.120 241 145 8 13 246 3 229 2.04e-23 96.3
MS.gene065972.t1 MTR_1g077540 32.444 225 143 5 38 254 20 243 3.01e-23 95.9
MS.gene065972.t1 MTR_6g069040 32.159 227 141 5 38 254 20 243 5.97e-23 95.1
MS.gene065972.t1 MTR_1g006590 31.102 254 148 11 13 255 3 240 1.24e-22 94.0
MS.gene065972.t1 MTR_3g118010 31.222 221 137 5 37 242 35 255 1.65e-22 94.4
MS.gene065972.t1 MTR_7g103030 33.180 217 135 6 38 246 20 234 3.74e-21 90.1
MS.gene065972.t1 MTR_1g115405 32.759 232 142 7 38 257 20 249 6.95e-21 89.4
MS.gene065972.t1 MTR_2g094270 30.837 227 133 6 39 242 40 265 1.48e-20 89.4
MS.gene065972.t1 MTR_7g101190 30.357 224 133 6 37 238 37 259 4.42e-20 87.8
MS.gene065972.t1 MTR_4g063090 32.287 223 142 5 44 258 26 247 5.41e-20 87.0
MS.gene065972.t1 MTR_4g415300 28.409 264 164 8 1 248 26 280 1.13e-19 86.7
MS.gene065972.t1 MTR_8g098375 28.369 282 173 9 2 259 11 287 2.79e-19 85.5
MS.gene065972.t1 MTR_4g059390 30.568 229 134 7 37 242 37 263 1.12e-18 84.0
MS.gene065972.t1 MTR_5g082070 29.707 239 145 7 37 256 53 287 1.38e-18 84.0
MS.gene065972.t1 MTR_5g010150 29.289 239 145 7 30 248 43 277 1.42e-18 83.6
MS.gene065972.t1 MTR_3g070210 26.236 263 177 6 10 256 26 287 2.85e-18 82.8
MS.gene065972.t1 MTR_2g060360 30.045 223 148 4 38 253 18 239 1.27e-17 80.5
MS.gene065972.t1 MTR_2g094270 30.435 207 123 5 39 225 40 245 3.30e-17 79.7
MS.gene065972.t1 MTR_1g095070 30.220 182 109 4 82 248 25 203 1.32e-16 77.0
MS.gene065972.t1 MTR_3g070210 27.586 203 134 4 37 226 53 255 1.41e-14 72.4
MS.gene065972.t1 MTR_7g023350 27.706 231 160 3 38 261 20 250 2.17e-14 71.6
MS.gene065972.t1 MTR_1g095070 31.410 156 92 3 82 225 25 177 5.03e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065972.t1 AT4G18910 71.486 249 64 1 33 274 46 294 3.18e-131 374
MS.gene065972.t1 AT4G19030 68.045 266 78 1 8 266 24 289 8.75e-129 367
MS.gene065972.t1 AT5G37820 53.480 273 124 1 1 273 8 277 2.16e-101 298
MS.gene065972.t1 AT5G37820 53.480 273 124 1 1 273 44 313 7.17e-101 298
MS.gene065972.t1 AT5G37810 54.074 270 117 3 3 266 2 270 3.20e-100 295
MS.gene065972.t1 AT1G31885 52.399 271 118 2 1 260 1 271 2.03e-98 291
MS.gene065972.t1 AT2G34390 53.101 258 113 2 17 267 26 282 7.49e-91 271
MS.gene065972.t1 AT4G18910 73.446 177 40 1 33 202 46 222 1.29e-89 265
MS.gene065972.t1 AT4G19030 65.672 201 62 1 8 201 24 224 4.24e-88 261
MS.gene065972.t1 AT1G31885 55.760 217 89 1 51 260 1 217 2.23e-86 259
MS.gene065972.t1 AT2G34390 53.782 238 102 2 37 267 4 240 2.98e-85 255
MS.gene065972.t1 AT4G19030 63.187 182 60 1 8 182 24 205 3.19e-76 231
MS.gene065972.t1 AT1G80760 50.820 244 113 3 12 253 55 293 3.80e-73 226
MS.gene065972.t1 AT4G10380 48.819 254 119 3 15 260 54 304 1.23e-72 225
MS.gene065972.t1 AT2G34390 55.959 193 77 2 17 202 26 217 1.53e-68 211
MS.gene065972.t1 AT2G34390 55.429 175 70 2 17 184 26 199 6.24e-62 195
MS.gene065972.t1 AT3G06100 38.057 247 150 3 10 254 16 261 8.88e-48 160
MS.gene065972.t1 AT3G06100 40.654 214 125 2 42 254 1 213 3.35e-47 157
MS.gene065972.t1 AT1G31885 48.936 141 65 2 1 134 1 141 1.47e-37 130
MS.gene065972.t1 AT3G16240 33.186 226 136 5 34 248 14 235 3.45e-29 111
MS.gene065972.t1 AT2G29870 52.688 93 44 0 154 246 20 112 7.57e-28 104
MS.gene065972.t1 AT5G47450 32.636 239 151 4 30 258 10 248 2.07e-27 106
MS.gene065972.t1 AT3G06100 38.298 141 85 2 115 254 41 180 2.82e-25 99.8
MS.gene065972.t1 AT4G17340 30.252 238 156 4 30 257 10 247 1.71e-24 99.0
MS.gene065972.t1 AT4G35100 31.579 228 141 5 30 242 29 256 3.63e-23 96.3
MS.gene065972.t1 AT4G35100 31.579 228 141 5 30 242 29 256 3.63e-23 96.3
MS.gene065972.t1 AT3G26520 33.486 218 138 3 38 248 21 238 6.81e-23 94.7
MS.gene065972.t1 AT1G17810 34.286 175 111 2 91 261 86 260 4.41e-22 92.8
MS.gene065972.t1 AT2G16850 30.769 221 138 5 37 242 34 254 5.49e-22 92.8
MS.gene065972.t1 AT2G36830 34.434 212 131 4 38 241 20 231 9.81e-22 91.7
MS.gene065972.t1 AT5G60660 29.362 235 143 6 36 248 36 269 6.57e-21 90.1
MS.gene065972.t1 AT2G39010 29.060 234 143 6 37 248 36 268 1.03e-20 89.7
MS.gene065972.t1 AT2G25810 32.420 219 135 7 38 248 18 231 2.86e-20 87.8
MS.gene065972.t1 AT1G73190 35.542 166 103 3 92 254 87 251 2.97e-20 88.2
MS.gene065972.t1 AT4G23400 28.629 248 155 6 30 259 44 287 9.09e-20 87.0
MS.gene065972.t1 AT2G37170 28.879 232 142 6 39 248 37 267 1.07e-19 86.7
MS.gene065972.t1 AT2G37170 28.879 232 142 6 39 248 80 310 1.27e-19 87.4
MS.gene065972.t1 AT3G61430 29.150 247 151 7 30 257 43 284 2.07e-19 85.9
MS.gene065972.t1 AT3G61430 29.150 247 151 7 30 257 43 284 2.07e-19 85.9
MS.gene065972.t1 AT4G01470 35.465 172 107 3 80 248 67 237 2.82e-19 85.1
MS.gene065972.t1 AT3G54820 28.326 233 146 5 37 248 36 268 3.86e-19 85.5
MS.gene065972.t1 AT1G01620 29.412 238 145 5 30 248 43 276 3.97e-19 85.1
MS.gene065972.t1 AT3G53420 28.755 233 145 6 37 248 37 269 4.32e-19 85.1
MS.gene065972.t1 AT3G53420 28.755 233 145 6 37 248 37 269 4.32e-19 85.1
MS.gene065972.t1 AT4G00430 27.509 269 170 6 2 248 12 277 6.08e-19 84.7
MS.gene065972.t1 AT2G45960 28.745 247 152 7 30 257 43 284 9.51e-19 84.3
MS.gene065972.t1 AT3G47440 29.224 219 145 4 38 248 22 238 2.74e-18 82.4
MS.gene065972.t1 AT2G37180 28.448 232 143 6 39 248 37 267 1.44e-17 80.9
MS.gene065972.t1 AT1G01620 31.720 186 107 4 69 238 13 194 1.56e-16 76.6
MS.gene065972.t1 AT2G45960 30.476 210 126 5 30 223 43 248 2.64e-16 77.4
MS.gene065972.t1 AT2G45960 30.476 210 126 5 30 223 43 248 2.74e-16 77.4
MS.gene065972.t1 AT2G39010 29.936 157 96 4 37 180 36 191 9.81e-12 63.5

Find 72 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTTATGTTTACCATTTC+TGG 0.053365 4.3:-13332492 MS.gene065972:CDS
ATATTTGTTGTCGCCGATTT+TGG 0.136028 4.3:-13332051 MS.gene065972:CDS
TTCCAATTTCTTAAACTATT+TGG 0.141022 4.3:+13331919 None:intergenic
TCCTGGGATTGCAATTGTTT+GGG 0.194809 4.3:-13334038 MS.gene065972:CDS
TTCATTGATGCTCCTGTTAC+CGG 0.292955 4.3:+13332131 None:intergenic
TTCCTGGGATTGCAATTGTT+TGG 0.304422 4.3:-13334039 MS.gene065972:CDS
AAGGAGGTTTCCATTGTTAC+AGG 0.315994 4.3:-13333927 MS.gene065972:intron
ACATATTTCTTGATATTTGC+AGG 0.316106 4.3:-13334109 MS.gene065972:CDS
CTCTGTCCCTTTCTTGCAAA+AGG 0.359353 4.3:-13334267 MS.gene065972:intron
GACAACGTGGTAACACTTCC+TGG 0.380447 4.3:-13334055 MS.gene065972:CDS
CACCACGCCCAGCTTCTTTG+AGG 0.393307 4.3:+13331946 None:intergenic
TTATTTGACAGACCGGTAAC+AGG 0.396494 4.3:-13332143 MS.gene065972:intron
TTGCTTAATGTGATGATTGC+AGG 0.400582 4.3:-13332315 MS.gene065972:CDS
CAAGAAAGGGACAGAGATAA+AGG 0.403387 4.3:+13334274 None:intergenic
AATACAGTTAATAGCAGAAA+TGG 0.417887 4.3:-13334137 MS.gene065972:intron
GAGTTGGCTGGAATTGCAAT+TGG 0.418098 4.3:-13332348 MS.gene065972:CDS
TTGTAGATTGGTGAGTTGGC+TGG 0.424279 4.3:-13332360 MS.gene065972:intron
TACTCTCTCGGTCATATCTC+TGG 0.424586 4.3:-13333992 MS.gene065972:CDS
GCTGGTCCTAGAGTTCTAAC+CGG 0.437239 4.3:+13332107 None:intergenic
TTATATATCAGCTCAGCTCT+TGG 0.447897 4.3:-13332637 MS.gene065972:CDS
CATGGGTCTACAACACTGTT+AGG 0.451802 4.3:-13332013 MS.gene065972:CDS
CTTTGAGGAAGGATGCACTC+TGG 0.452465 4.3:+13331961 None:intergenic
ATTCAAGAACAAAAGCTTGA+AGG 0.459020 4.3:+13332529 None:intergenic
CTCCAGAAATGGTAAACATA+AGG 0.466289 4.3:+13332490 None:intergenic
GCATCCTTCCTCAAAGAAGC+TGG 0.467151 4.3:-13331954 MS.gene065972:CDS
ATCGGTGGCAACTCCAGAAA+TGG 0.467754 4.3:+13332479 None:intergenic
CCTGGGATTGCAATTGTTTG+GGG 0.472067 4.3:-13334037 MS.gene065972:CDS
TATATATCAGCTCAGCTCTT+GGG 0.473337 4.3:-13332636 MS.gene065972:CDS
TTTGAGGAAGGATGCACTCT+GGG 0.475411 4.3:+13331962 None:intergenic
AGTTGGCTGGAATTGCAATT+GGG 0.481359 4.3:-13332347 MS.gene065972:CDS
TGTTTGGGGACTAACTTTGT+TGG 0.484275 4.3:-13334023 MS.gene065972:CDS
GTAGACCCATGCTCCGGCGA+TGG 0.495881 4.3:+13332025 None:intergenic
TTGTATCTAAAACAACCTCA+TGG 0.499097 4.3:+13334333 None:intergenic
CGAGTTTACCGCTCTAGTAT+CGG 0.500638 4.3:+13332461 None:intergenic
TTGGTATTGATTTACTCTCT+CGG 0.501228 4.3:-13334004 MS.gene065972:CDS
CCCCAAACAATTGCAATCCC+AGG 0.504953 4.3:+13334037 None:intergenic
TACTCATTGTGTACAAAAGC+TGG 0.505829 4.3:+13332089 None:intergenic
ACAATGAGTACAGAGGAATA+TGG 0.514676 4.3:-13332076 MS.gene065972:CDS
ATGAACCCGGTTAGAACTCT+AGG 0.519479 4.3:-13332113 MS.gene065972:CDS
CTCAAAGAAGCTGGGCGTGG+TGG 0.520816 4.3:-13331945 MS.gene065972:CDS
TTTCAGGAACACTTCCATCT+GGG 0.526344 4.3:-13332557 MS.gene065972:CDS
TTCCTCAAAGAAGCTGGGCG+TGG 0.532484 4.3:-13331948 MS.gene065972:CDS
TGTATCTAAAACAACCTCAT+GGG 0.536127 4.3:+13334334 None:intergenic
AGCTGAGCTGATATATAAGC+TGG 0.541389 4.3:+13332642 None:intergenic
TCACAAACATGTGAAGGCTC+TGG 0.541682 4.3:-13334299 MS.gene065972:CDS
TCAAAGAAGCTGGGCGTGGT+GGG 0.548515 4.3:-13331944 MS.gene065972:CDS
GCAAAAGCAATAGTAACAGC+AGG 0.549342 4.3:+13333956 None:intergenic
CTCTGGGTGATCTCACGCAA+TGG 0.559604 4.3:+13331978 None:intergenic
ACGCCCAGCTTCTTTGAGGA+AGG 0.561104 4.3:+13331950 None:intergenic
CTGGTCCTAGAGTTCTAACC+GGG 0.561868 4.3:+13332108 None:intergenic
CATCCTTCCTCAAAGAAGCT+GGG 0.568992 4.3:-13331953 MS.gene065972:CDS
GGTAAACATAAGGTAAAATG+TGG 0.569076 4.3:+13332500 None:intergenic
ATGGAAGTGTTCCTGAAAAG+TGG 0.569487 4.3:+13332562 None:intergenic
TGCTTAATGTGATGATTGCA+GGG 0.570893 4.3:-13332314 MS.gene065972:intron
ACAACGTGGTAACACTTCCT+GGG 0.584721 4.3:-13334054 MS.gene065972:CDS
ACAGTTAATAGCAGAAATGG+TGG 0.598028 4.3:-13334134 MS.gene065972:intron
AGTGTTGTAGACCCATGCTC+CGG 0.603956 4.3:+13332019 None:intergenic
GCTTGAAGGTTAGACCCAGA+TGG 0.605252 4.3:+13332543 None:intergenic
TTGGGAGCAACACTTGCTAG+TGG 0.608245 4.3:-13332618 MS.gene065972:CDS
ACTCTAAAACTAATATTCAG+TGG 0.608319 4.3:-13332594 MS.gene065972:CDS
GATTCCTCACAAACATGTGA+AGG 0.617028 4.3:-13334305 MS.gene065972:CDS
GTAGTGTTGACCTGTAACAA+TGG 0.623661 4.3:+13333917 None:intergenic
TGCTTCAATAGTAGTGAACA+AGG 0.624250 4.3:-13334083 MS.gene065972:CDS
AACAGGAGCATCAATGAACC+CGG 0.626218 4.3:-13332126 MS.gene065972:CDS
AGAGCCTTCACATGTTTGTG+AGG 0.626868 4.3:+13334301 None:intergenic
CAGTTAATAGCAGAAATGGT+GGG 0.636357 4.3:-13334133 MS.gene065972:CDS
GTTTACCGCTCTAGTATCGG+TGG 0.680324 4.3:+13332464 None:intergenic
AGCAAGAATTGAAACCCATG+AGG 0.683901 4.3:-13334348 MS.gene065972:CDS
TTTGTACACAATGAGTACAG+AGG 0.696529 4.3:-13332083 MS.gene065972:CDS
TAACAATGGAAACCTCCTTG+TGG 0.700560 4.3:+13333931 None:intergenic
AGTTGCCACCGATACTAGAG+CGG 0.735236 4.3:-13332469 MS.gene065972:intron
GAACAAGGACAACGACAACG+TGG 0.744853 4.3:-13334068 MS.gene065972:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAATATATAAAAAAT+TGG - chr4.3:13333302-13333321 MS.gene065972:intron 0.0%
!! ATTTACAAAAATAAACTTTA+TGG + chr4.3:13332419-13332438 None:intergenic 10.0%
!! TATAGTATATTGAATTAATT+AGG - chr4.3:13332118-13332137 MS.gene065972:CDS 10.0%
!!! AAAAAGATTATTTTTTTTAC+AGG + chr4.3:13332905-13332924 None:intergenic 10.0%
!! AAAAATACATGATAAATCAA+TGG - chr4.3:13332968-13332987 MS.gene065972:intron 15.0%
!! TTTACAAATTGAGTTAAAAA+GGG + chr4.3:13332656-13332675 None:intergenic 15.0%
!!! AGTTTTGCAATTTTTTTTTA+TGG + chr4.3:13334058-13334077 None:intergenic 15.0%
!!! ATATTTTATGTTTTGTAGAT+TGG - chr4.3:13333922-13333941 MS.gene065972:intron 15.0%
!!! TGAAGAGTTTTTTTTTTTTT+TGG + chr4.3:13332931-13332950 None:intergenic 15.0%
!!! TGATATTTTCATTTAAAATC+AGG + chr4.3:13333227-13333246 None:intergenic 15.0%
!!! TTGATTTAAAATTAATGTCA+AGG + chr4.3:13333259-13333278 None:intergenic 15.0%
!!! TTTTACAAATTGAGTTAAAA+AGG + chr4.3:13332657-13332676 None:intergenic 15.0%
!! AATTAAGTAACATGATTTCT+TGG + chr4.3:13333562-13333581 None:intergenic 20.0%
!! ACCTATAAGAATTATACAAA+GGG + chr4.3:13333631-13333650 None:intergenic 20.0%
!! CCAAAATTCTTCAATTTATT+AGG - chr4.3:13333480-13333499 MS.gene065972:intron 20.0%
!! TACCTATAAGAATTATACAA+AGG + chr4.3:13333632-13333651 None:intergenic 20.0%
!! TTACAAATTGAGTTAAAAAG+GGG + chr4.3:13332655-13332674 None:intergenic 20.0%
!!! ATTCAACGAGTATTTTTATT+TGG + chr4.3:13333520-13333539 None:intergenic 20.0%
!!! CCTAATAAATTGAAGAATTT+TGG + chr4.3:13333483-13333502 None:intergenic 20.0%
!!! GTATTTTTACAATGATAAGT+TGG + chr4.3:13332956-13332975 None:intergenic 20.0%
!!! TAGTAATTTTGCATTTAACT+AGG + chr4.3:13334028-13334047 None:intergenic 20.0%
!!! TTAATTAGGTTTTGTTTTGT+TGG - chr4.3:13332132-13332151 MS.gene065972:CDS 20.0%
!!! TTCAACGAGTATTTTTATTT+GGG + chr4.3:13333519-13333538 None:intergenic 20.0%
!!! TTTTTATTTGGGAGTTTTTA+TGG + chr4.3:13333508-13333527 None:intergenic 20.0%
! AAAGAGTCTTAATTTCTTGT+GGG + chr4.3:13332600-13332619 None:intergenic 25.0%
! AATACAGTTAATAGCAGAAA+TGG - chr4.3:13332157-13332176 MS.gene065972:intron 25.0%
! ACATATTTCTTGATATTTGC+AGG - chr4.3:13332185-13332204 MS.gene065972:intron 25.0%
! TAAAGAGTCTTAATTTCTTG+TGG + chr4.3:13332601-13332620 None:intergenic 25.0%
! TACAAATTGAGTTAAAAAGG+GGG + chr4.3:13332654-13332673 None:intergenic 25.0%
! TCCCTTTGTATAATTCTTAT+AGG - chr4.3:13333627-13333646 MS.gene065972:intron 25.0%
! TGATTCATAATTGCATAACA+TGG + chr4.3:13333607-13333626 None:intergenic 25.0%
! TTAGAATGATTCATTACTCT+CGG - chr4.3:13333114-13333133 MS.gene065972:intron 25.0%
! TTAGGTAACCCTTAAAATTT+GGG + chr4.3:13333186-13333205 None:intergenic 25.0%
! TTTAGGTAACCCTTAAAATT+TGG + chr4.3:13333187-13333206 None:intergenic 25.0%
!! ACAGACTTAAGTTTTTACAA+AGG + chr4.3:13332879-13332898 None:intergenic 25.0%
!! TAATCCAACCCAAATTTTAA+GGG - chr4.3:13333175-13333194 MS.gene065972:intron 25.0%
!! TCTCATTGGTAATAAAATGA+TGG - chr4.3:13333331-13333350 MS.gene065972:intron 25.0%
!! TTAATCCAACCCAAATTTTA+AGG - chr4.3:13333174-13333193 MS.gene065972:intron 25.0%
!! TTTTCAATTTCAAGAGAAGT+GGG + chr4.3:13332627-13332646 None:intergenic 25.0%
!!! ACTCTAAAACTAATATTCAG+TGG - chr4.3:13333700-13333719 MS.gene065972:intron 25.0%
!!! ATTTAACTAGGTTCTAGATA+GGG + chr4.3:13334016-13334035 None:intergenic 25.0%
!!! CACAGTTGGTAAATTTTAAT+TGG + chr4.3:13332811-13332830 None:intergenic 25.0%
!!! TTCTTGATTAGCTACTTTTT+CGG - chr4.3:13332558-13332577 MS.gene065972:CDS 25.0%
AAAATTGGCATAACTCTCAT+TGG - chr4.3:13333317-13333336 MS.gene065972:intron 30.0%
ACAAATTGAGTTAAAAAGGG+GGG + chr4.3:13332653-13332672 None:intergenic 30.0%
ATTCAAGAACAAAAGCTTGA+AGG + chr4.3:13333768-13333787 None:intergenic 30.0%
CTATGTGTATGAGTTTGTAA+AGG + chr4.3:13332068-13332087 None:intergenic 30.0%
GAGTTTGTAAAGGAAAGATT+AGG + chr4.3:13332058-13332077 None:intergenic 30.0%
GGTAAACATAAGGTAAAATG+TGG + chr4.3:13333797-13333816 None:intergenic 30.0%
TACCTTATGTTTACCATTTC+TGG - chr4.3:13333802-13333821 MS.gene065972:intron 30.0%
TTCATAATTGCATAACATGG+TGG + chr4.3:13333604-13333623 None:intergenic 30.0%
! AATGCAGAGTATTAGTGTTT+AGG + chr4.3:13333204-13333223 None:intergenic 30.0%
! GTTTTCAATTTCAAGAGAAG+TGG + chr4.3:13332628-13332647 None:intergenic 30.0%
! TGTATCTAAAACAACCTCAT+GGG + chr4.3:13331963-13331982 None:intergenic 30.0%
! TTGTATCTAAAACAACCTCA+TGG + chr4.3:13331964-13331983 None:intergenic 30.0%
! TTTTCGGTATAATTATCTGC+AGG - chr4.3:13332574-13332593 MS.gene065972:CDS 30.0%
!! CATTTAACTAGGTTCTAGAT+AGG + chr4.3:13334017-13334036 None:intergenic 30.0%
!! TTGGTATTGATTTACTCTCT+CGG - chr4.3:13332290-13332309 MS.gene065972:intron 30.0%
!!! AGAATTTTTCAAGACACAGT+TGG + chr4.3:13332825-13332844 None:intergenic 30.0%
!!! CACTTTTTTATTTGACAGAC+CGG - chr4.3:13334144-13334163 MS.gene065972:intron 30.0%
!!! GGTTGTTTTAGATACAAACA+AGG - chr4.3:13331967-13331986 MS.gene065972:CDS 30.0%
ACAATGAGTACAGAGGAATA+TGG - chr4.3:13334218-13334237 MS.gene065972:intron 35.0%
ACAGTTAATAGCAGAAATGG+TGG - chr4.3:13332160-13332179 MS.gene065972:intron 35.0%
CAGTTAATAGCAGAAATGGT+GGG - chr4.3:13332161-13332180 MS.gene065972:intron 35.0%
CTCCAGAAATGGTAAACATA+AGG + chr4.3:13333807-13333826 None:intergenic 35.0%
GGAGATATCACTTTACAACT+CGG - chr4.3:13333135-13333154 MS.gene065972:intron 35.0%
GTAACCCTTAAAATTTGGGT+TGG + chr4.3:13333182-13333201 None:intergenic 35.0%
GTGATGTTTCTTAAAGCACA+TGG - chr4.3:13333397-13333416 MS.gene065972:intron 35.0%
TACTCATTGTGTACAAAAGC+TGG + chr4.3:13334208-13334227 None:intergenic 35.0%
TATATATCAGCTCAGCTCTT+GGG - chr4.3:13333658-13333677 MS.gene065972:intron 35.0%
TGCTTAATGTGATGATTGCA+GGG - chr4.3:13333980-13333999 MS.gene065972:CDS 35.0%
TGCTTCAATAGTAGTGAACA+AGG - chr4.3:13332211-13332230 MS.gene065972:intron 35.0%
TTATATATCAGCTCAGCTCT+TGG - chr4.3:13333657-13333676 MS.gene065972:intron 35.0%
TTGCTTAATGTGATGATTGC+AGG - chr4.3:13333979-13333998 MS.gene065972:CDS 35.0%
TTTGTACACAATGAGTACAG+AGG - chr4.3:13334211-13334230 MS.gene065972:intron 35.0%
! TGTTTTGTAGATTGGTGAGT+TGG - chr4.3:13333930-13333949 MS.gene065972:CDS 35.0%
!!! ATATTTGTTGTCGCCGATTT+TGG - chr4.3:13334243-13334262 MS.gene065972:intron 35.0%
!!! TATTTGTTGTCGCCGATTTT+GGG - chr4.3:13334244-13334263 MS.gene065972:intron 35.0%
AAGGAGGTTTCCATTGTTAC+AGG - chr4.3:13332367-13332386 MS.gene065972:intron 40.0%
AGCAAGAATTGAAACCCATG+AGG - chr4.3:13331946-13331965 MS.gene065972:CDS 40.0%
AGCTGAGCTGATATATAAGC+TGG + chr4.3:13333655-13333674 None:intergenic 40.0%
AGTTGGCTGGAATTGCAATT+GGG - chr4.3:13333947-13333966 MS.gene065972:CDS 40.0%
CAAGAAAGGGACAGAGATAA+AGG + chr4.3:13332023-13332042 None:intergenic 40.0%
CTATTTGCCACAATTCAAGG+AGG - chr4.3:13332749-13332768 MS.gene065972:intron 40.0%
GATTCCTCACAAACATGTGA+AGG - chr4.3:13331989-13332008 MS.gene065972:CDS 40.0%
GCAAAAGCAATAGTAACAGC+AGG + chr4.3:13332341-13332360 None:intergenic 40.0%
GCACTATTTGCCACAATTCA+AGG - chr4.3:13332746-13332765 MS.gene065972:intron 40.0%
GGCAAATAGTGCATTGTGAT+TGG + chr4.3:13332738-13332757 None:intergenic 40.0%
TAACAATGGAAACCTCCTTG+TGG + chr4.3:13332366-13332385 None:intergenic 40.0%
TCCTGGGATTGCAATTGTTT+GGG - chr4.3:13332256-13332275 MS.gene065972:intron 40.0%
TGTTTGGGGACTAACTTTGT+TGG - chr4.3:13332271-13332290 MS.gene065972:intron 40.0%
TTATTTGACAGACCGGTAAC+AGG - chr4.3:13334151-13334170 MS.gene065972:intron 40.0%
TTCATTGATGCTCCTGTTAC+CGG + chr4.3:13334166-13334185 None:intergenic 40.0%
TTCCTGGGATTGCAATTGTT+TGG - chr4.3:13332255-13332274 MS.gene065972:intron 40.0%
TTTCAGGAACACTTCCATCT+GGG - chr4.3:13333737-13333756 MS.gene065972:intron 40.0%
! GAGTCACCTTTTGCAAGAAA+GGG + chr4.3:13332036-13332055 None:intergenic 40.0%
!! ATGGAAGTGTTCCTGAAAAG+TGG + chr4.3:13333735-13333754 None:intergenic 40.0%
!! CTATTGCTTTTGCTACCACA+AGG - chr4.3:13332348-13332367 MS.gene065972:CDS 40.0%
!! GTAGTGTTGACCTGTAACAA+TGG + chr4.3:13332380-13332399 None:intergenic 40.0%
!! TTTTCAGGAACACTTCCATC+TGG - chr4.3:13333736-13333755 MS.gene065972:intron 40.0%
!!! ATTTGTTGTCGCCGATTTTG+GGG - chr4.3:13334245-13334264 MS.gene065972:intron 40.0%
ACAACGTGGTAACACTTCCT+GGG - chr4.3:13332240-13332259 MS.gene065972:intron 45.0%
AGAGCCTTCACATGTTTGTG+AGG + chr4.3:13331996-13332015 None:intergenic 45.0%
ATGAACCCGGTTAGAACTCT+AGG - chr4.3:13334181-13334200 MS.gene065972:intron 45.0%
CATCCTTCCTCAAAGAAGCT+GGG - chr4.3:13334341-13334360 MS.gene065972:CDS 45.0%
CATGGGTCTACAACACTGTT+AGG - chr4.3:13334281-13334300 MS.gene065972:CDS 45.0%
CCTGGGATTGCAATTGTTTG+GGG - chr4.3:13332257-13332276 MS.gene065972:intron 45.0%
CGAGTTTACCGCTCTAGTAT+CGG + chr4.3:13333836-13333855 None:intergenic 45.0%
CTCTGTCCCTTTCTTGCAAA+AGG - chr4.3:13332027-13332046 MS.gene065972:CDS 45.0%
GAGTTGGCTGGAATTGCAAT+TGG - chr4.3:13333946-13333965 MS.gene065972:CDS 45.0%
TACTCTCTCGGTCATATCTC+TGG - chr4.3:13332302-13332321 MS.gene065972:intron 45.0%
TCACAAACATGTGAAGGCTC+TGG - chr4.3:13331995-13332014 MS.gene065972:CDS 45.0%
TTTGAGGAAGGATGCACTCT+GGG + chr4.3:13334335-13334354 None:intergenic 45.0%
! AGGAGGTGTGGCTGAAAAAT+AGG - chr4.3:13332766-13332785 MS.gene065972:intron 45.0%
! GGAGTCACCTTTTGCAAGAA+AGG + chr4.3:13332037-13332056 None:intergenic 45.0%
! TTGTAGATTGGTGAGTTGGC+TGG - chr4.3:13333934-13333953 MS.gene065972:CDS 45.0%
!! AACAGGAGCATCAATGAACC+CGG - chr4.3:13334168-13334187 MS.gene065972:intron 45.0%
!! TTGCTTTTGCTACCACAAGG+AGG - chr4.3:13332351-13332370 MS.gene065972:CDS 45.0%
!!! TTTGTTGTCGCCGATTTTGG+GGG - chr4.3:13334246-13334265 MS.gene065972:intron 45.0%
!!! AATTTTTTTTTTTTTAATCT+TGG - chr4.3:13332534-13332553 MS.gene065972:CDS 5.0%
!!! TTTTTTTTACAAAAAAATTA+TGG - chr4.3:13333361-13333380 MS.gene065972:intron 5.0%
AGCCACACCTCCTTGAATTG+TGG + chr4.3:13332759-13332778 None:intergenic 50.0%
AGTGTTGTAGACCCATGCTC+CGG + chr4.3:13334278-13334297 None:intergenic 50.0%
AGTTGCCACCGATACTAGAG+CGG - chr4.3:13333825-13333844 MS.gene065972:intron 50.0%
ATCGGTGGCAACTCCAGAAA+TGG + chr4.3:13333818-13333837 None:intergenic 50.0%
CCCCAAACAATTGCAATCCC+AGG + chr4.3:13332260-13332279 None:intergenic 50.0%
GAACAAGGACAACGACAACG+TGG - chr4.3:13332226-13332245 MS.gene065972:intron 50.0%
GACAACGTGGTAACACTTCC+TGG - chr4.3:13332239-13332258 MS.gene065972:intron 50.0%
GCATCCTTCCTCAAAGAAGC+TGG - chr4.3:13334340-13334359 MS.gene065972:CDS 50.0%
GCTTGAAGGTTAGACCCAGA+TGG + chr4.3:13333754-13333773 None:intergenic 50.0%
TGCCACAATTCAAGGAGGTG+TGG - chr4.3:13332754-13332773 MS.gene065972:intron 50.0%
! CTGGTCCTAGAGTTCTAACC+GGG + chr4.3:13334189-13334208 None:intergenic 50.0%
! CTTTGAGGAAGGATGCACTC+TGG + chr4.3:13334336-13334355 None:intergenic 50.0%
! GCTGGTCCTAGAGTTCTAAC+CGG + chr4.3:13334190-13334209 None:intergenic 50.0%
! GTTTACCGCTCTAGTATCGG+TGG + chr4.3:13333833-13333852 None:intergenic 50.0%
! TTGGGAGCAACACTTGCTAG+TGG - chr4.3:13333676-13333695 MS.gene065972:intron 50.0%
!! GGCACACATGACCACTTTTC+AGG - chr4.3:13333721-13333740 MS.gene065972:intron 50.0%
CTCTGGGTGATCTCACGCAA+TGG + chr4.3:13334319-13334338 None:intergenic 55.0%
TCAAAGAAGCTGGGCGTGGT+GGG - chr4.3:13334350-13334369 MS.gene065972:CDS 55.0%
TTCCTCAAAGAAGCTGGGCG+TGG - chr4.3:13334346-13334365 MS.gene065972:CDS 55.0%
! ACGCCCAGCTTCTTTGAGGA+AGG + chr4.3:13334347-13334366 None:intergenic 55.0%
CACCACGCCCAGCTTCTTTG+AGG + chr4.3:13334351-13334370 None:intergenic 60.0%
CTCAAAGAAGCTGGGCGTGG+TGG - chr4.3:13334349-13334368 MS.gene065972:CDS 60.0%
CCGGCGATGGCCCCCAAAAT+CGG + chr4.3:13334259-13334278 None:intergenic 65.0%
GTAGACCCATGCTCCGGCGA+TGG + chr4.3:13334272-13334291 None:intergenic 65.0%
!! CCGATTTTGGGGGCCATCGC+CGG - chr4.3:13334256-13334275 MS.gene065972:intron 65.0%
GGGGGCCATCGCCGGAGCAT+GGG - chr4.3:13334264-13334283 MS.gene065972:intron 75.0%
TGGGGGCCATCGCCGGAGCA+TGG - chr4.3:13334263-13334282 MS.gene065972:intron 75.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 13331929 13334387 13331929 ID=MS.gene065972
chr4.3 mRNA 13331929 13334387 13331929 ID=MS.gene065972.t1;Parent=MS.gene065972
chr4.3 exon 13334268 13334387 13334268 ID=MS.gene065972.t1.exon1;Parent=MS.gene065972.t1
chr4.3 CDS 13334268 13334387 13334268 ID=cds.MS.gene065972.t1;Parent=MS.gene065972.t1
chr4.3 exon 13333928 13334152 13333928 ID=MS.gene065972.t1.exon2;Parent=MS.gene065972.t1
chr4.3 CDS 13333928 13334152 13333928 ID=cds.MS.gene065972.t1;Parent=MS.gene065972.t1
chr4.3 exon 13332470 13332667 13332470 ID=MS.gene065972.t1.exon3;Parent=MS.gene065972.t1
chr4.3 CDS 13332470 13332667 13332470 ID=cds.MS.gene065972.t1;Parent=MS.gene065972.t1
chr4.3 exon 13332315 13332376 13332315 ID=MS.gene065972.t1.exon4;Parent=MS.gene065972.t1
chr4.3 CDS 13332315 13332376 13332315 ID=cds.MS.gene065972.t1;Parent=MS.gene065972.t1
chr4.3 exon 13331929 13332154 13331929 ID=MS.gene065972.t1.exon5;Parent=MS.gene065972.t1
chr4.3 CDS 13331929 13332154 13331929 ID=cds.MS.gene065972.t1;Parent=MS.gene065972.t1
Gene Sequence

>MS.gene065972

ATGGCAAATGATAATTCAGCAAGAATTGAAACCCATGAGGTTGTTTTAGATACAAACAAGGATTCCTCACAAACATGTGAAGGCTCTGGCACCTTTATCTCTGTCCCTTTCTTGCAAAAGGTGACTCCTAATCTTTCCTTTACAAACTCATACACATAGTTACATAGTTTCTTTCTAAACTTTTGATCATATAGTATATTGAATTAATTAGGTTTTGTTTTGTTGGTGAATACAGTTAATAGCAGAAATGGTGGGCACATATTTCTTGATATTTGCAGGATGTGCTTCAATAGTAGTGAACAAGGACAACGACAACGTGGTAACACTTCCTGGGATTGCAATTGTTTGGGGACTAACTTTGTTGGTATTGATTTACTCTCTCGGTCATATCTCTGGTGCTCATTTCAATCCTGCTGTTACTATTGCTTTTGCTACCACAAGGAGGTTTCCATTGTTACAGGTCAACACTACACACAATCTTTTTTCTCCATAAAGTTTATTTTTGTAAATAATATAATATTTTGACATACAAAGTTGATAACATATATACTCTATTTACTTTATTTAAATGTATATACTTTCTAATAATACAAGATAGACTATTTAATTTTTTTTTTTTTAATCTTGGCTTCTTGATTAGCTACTTTTTCGGTATAATTATCTGCAGGCCCACAAGAAATTAAGACTCTTTAAAGCCCACTTCTCTTGAAATTGAAAACTACCCCCCTTTTTAACTCAATTTGTAAAAAAGCAATGCTATATCTCTAGAAAAATAACATCAAGAATTCTTCAAGAATAAAGAATAGCCAATCACAATGCACTATTTGCCACAATTCAAGGAGGTGTGGCTGAAAAATAGGAAGTTGACAAATAAATCATCCAATTAAAATTTACCAACTGTGTCTTGAAAAATTCTTGAAGCTATTTTTCTTGAGAAGTAGCATTTTCCTTTGTAAAAACTTAAGTCTGTTATCCTGTAAAAAAAATAATCTTTTTTTACCAAAAAAAAAAAAAACTCTTCACGCCAACTTATCATTGTAAAAATACATGATAAATCAATGGTTTAAAAAGTTATCATTTATAAAAAAGTTACACAATATCATTTTCAAAATGTCAAAATGTAATAATAACTAAATTATATATTGAAAATGATAAAATAGAGACAATATATAGAATAACAAGTGATTAGAATGATTCATTACTCTCGGAGATATCACTTTACAACTCGGTTTTGTATGATAGTACTTAATCCAACCCAAATTTTAAGGGTTACCTAAACACTAATACTCTGCATTCCTGATTTTAAATGAAAATATCAGACAAATAACCTTGACATTAATTTTAAATCAATAATAGTGAATTCTAAATATTTTAAAAAAAATATATAAAAAATTGGCATAACTCTCATTGGTAATAAAATGATGGTAATTGTTTTTTTTTACAAAAAAATTATGGTTATTTTTATTAAGTGATGTTTCTTAAAGCACATGGATTTACAAATAGTTCTTTGATTAAAAAACATTCTATTATTTATTGATTGAGAAAATTATACCAAAATTCTTCAATTTATTAGGTACCATAAAAACTCCCAAATAAAAATACTCGTTGAATAAGTAGCTAAAATGTTTCTCCAAGAAATCATGTTACTTAATTTGTTCAAAAATTATTTTTACCACCATGTTATGCAATTATGAATCATCCCTTTGTATAATTCTTATAGGTACCAGCTTATATATCAGCTCAGCTCTTGGGAGCAACACTTGCTAGTGGAACTCTAAAACTAATATTCAGTGGCACACATGACCACTTTTCAGGAACACTTCCATCTGGGTCTAACCTTCAAGCTTTTGTTCTTGAATTCATAACCACATTTTACCTTATGTTTACCATTTCTGGAGTTGCCACCGATACTAGAGCGGTAAACTCGTCAAACACATCGATCTCATGTACATATAAACGTTCTTTAATACTTTTATTCATTATTTTTATTTTCATATTTTATGTTTTGTAGATTGGTGAGTTGGCTGGAATTGCAATTGGGTCTACATTATTGCTTAATGTGATGATTGCAGGGTATGTTTCTACCCTATCTAGAACCTAGTTAAATGCAAAATTACTAATATGTTCCATAAAAAAAAATTGCAAAACTAAGAGTTAGATATTTATGTCGAATCTAATTTATCTGTATAACACGAATTTGTAAAATGAGACGTGTCACTTTTTTATTTGACAGACCGGTAACAGGAGCATCAATGAACCCGGTTAGAACTCTAGGACCAGCTTTTGTACACAATGAGTACAGAGGAATATGGATATATTTGTTGTCGCCGATTTTGGGGGCCATCGCCGGAGCATGGGTCTACAACACTGTTAGGTACACTAACAAGCCATTGCGTGAGATCACCCAGAGTGCATCCTTCCTCAAAGAAGCTGGGCGTGGTGGGAACCAAATAGTTTAA
Protein sequence

>MS.gene065972.t1

MANDNSARIETHEVVLDTNKDSSQTCEGSGTFISVPFLQKLIAEMVGTYFLIFAGCASIVVNKDNDNVVTLPGIAIVWGLTLLVLIYSLGHISGAHFNPAVTIAFATTRRFPLLQVPAYISAQLLGATLASGTLKLIFSGTHDHFSGTLPSGSNLQAFVLEFITTFYLMFTISGVATDTRAIGELAGIAIGSTLLLNVMIAGPVTGASMNPVRTLGPAFVHNEYRGIWIYLLSPILGAIAGAWVYNTVRYTNKPLREITQSASFLKEAGRGGNQIV