AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27853

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27853.t1 MTR_8g087720 98.141 269 5 0 1 269 1 269 0.0 530
MS.gene27853.t1 MTR_2g017570 76.119 268 64 0 1 268 1 268 5.73e-151 422
MS.gene27853.t1 MTR_2g017610 76.119 268 64 0 1 268 1 268 6.26e-151 422
MS.gene27853.t1 MTR_8g087710 73.585 265 70 0 3 267 4 268 1.10e-150 422
MS.gene27853.t1 MTR_2g017590 75.769 260 63 0 7 266 5 264 1.34e-147 414
MS.gene27853.t1 MTR_2g017620 72.075 265 74 0 1 265 1 265 3.66e-140 395
MS.gene27853.t1 MTR_8g087720 98.010 201 4 0 1 201 1 201 1.15e-139 391
MS.gene27853.t1 MTR_2g017620 74.104 251 65 0 15 265 1 251 1.17e-136 385
MS.gene27853.t1 MTR_2g017660 64.364 275 91 2 1 268 1 275 1.11e-132 376
MS.gene27853.t1 MTR_8g087720 96.196 184 7 0 1 184 1 184 5.95e-125 353
MS.gene27853.t1 MTR_4g084880 62.931 232 84 1 37 266 34 265 8.41e-109 316
MS.gene27853.t1 MTR_2g017620 79.348 184 38 0 82 265 1 184 3.21e-106 306
MS.gene27853.t1 MTR_5g079570 54.867 226 101 1 28 253 30 254 3.08e-87 261
MS.gene27853.t1 MTR_3g008920 53.279 244 111 2 28 269 16 258 1.37e-86 259
MS.gene27853.t1 MTR_7g010650 49.402 251 125 2 1 251 1 249 1.82e-79 241
MS.gene27853.t1 MTR_4g006730 53.778 225 103 1 38 262 75 298 1.29e-76 235
MS.gene27853.t1 MTR_2g104940 51.111 225 109 1 38 262 80 303 2.98e-75 231
MS.gene27853.t1 MTR_4g006630 53.774 212 97 1 51 262 1 211 8.60e-73 222
MS.gene27853.t1 MTR_4g006730 53.302 212 98 1 51 262 1 211 3.95e-72 220
MS.gene27853.t1 MTR_1g097840 49.356 233 107 3 35 259 71 300 4.98e-69 215
MS.gene27853.t1 MTR_5g063930 45.349 258 124 5 4 255 8 254 3.13e-67 209
MS.gene27853.t1 MTR_4g006730 55.488 164 72 1 38 201 75 237 4.06e-51 168
MS.gene27853.t1 MTR_1g070385 38.073 218 134 1 35 252 59 275 4.03e-46 165
MS.gene27853.t1 MTR_1g097840 50.000 172 79 2 33 201 69 236 9.83e-46 154
MS.gene27853.t1 MTR_7g010650 44.199 181 99 2 1 181 1 179 2.95e-44 149
MS.gene27853.t1 MTR_4g006650 50.000 144 58 3 39 180 5 136 3.70e-32 120
MS.gene27853.t1 MTR_8g013680 31.915 235 149 4 26 252 10 241 1.69e-25 101
MS.gene27853.t1 MTR_2g101370 32.653 245 152 4 26 263 11 249 2.62e-25 100
MS.gene27853.t1 MTR_1g077540 31.624 234 151 4 27 253 12 243 9.35e-25 99.8
MS.gene27853.t1 MTR_5g012810 32.787 244 150 6 23 256 7 246 5.26e-23 94.7
MS.gene27853.t1 MTR_1g006490 32.093 215 137 6 37 245 18 229 3.74e-22 92.4
MS.gene27853.t1 MTR_1g006590 31.034 232 150 6 19 245 3 229 4.65e-21 89.7
MS.gene27853.t1 MTR_6g069040 29.461 241 147 6 27 253 12 243 7.83e-21 89.0
MS.gene27853.t1 MTR_3g118010 30.303 231 140 5 35 247 34 261 4.33e-19 84.7
MS.gene27853.t1 MTR_7g101190 28.879 232 144 5 27 237 28 259 1.15e-18 84.0
MS.gene27853.t1 MTR_4g063090 31.416 226 143 4 43 256 26 251 1.55e-18 82.8
MS.gene27853.t1 MTR_7g103030 30.802 237 143 7 23 247 9 236 8.80e-18 80.9
MS.gene27853.t1 MTR_1g115405 32.068 237 147 7 37 263 20 252 9.36e-18 80.9
MS.gene27853.t1 MTR_2g094270 28.821 229 145 5 32 247 48 271 6.11e-17 79.0
MS.gene27853.t1 MTR_5g010150 26.201 229 153 6 35 247 49 277 1.27e-16 78.2
MS.gene27853.t1 MTR_4g415300 27.193 228 151 6 35 247 53 280 1.30e-16 78.2
MS.gene27853.t1 MTR_8g098375 27.500 240 157 7 35 258 49 287 1.97e-16 77.4
MS.gene27853.t1 MTR_3g070210 28.261 230 147 7 35 247 52 280 2.19e-16 77.4
MS.gene27853.t1 MTR_5g082070 28.448 232 143 8 35 247 52 279 2.61e-16 77.4
MS.gene27853.t1 MTR_2g060360 28.761 226 147 5 37 252 18 239 8.21e-16 75.5
MS.gene27853.t1 MTR_4g059390 27.426 237 145 6 35 247 36 269 6.31e-15 73.2
MS.gene27853.t1 MTR_7g023350 27.155 232 162 2 37 261 20 251 2.68e-13 68.2
MS.gene27853.t1 MTR_2g094270 29.082 196 124 4 32 217 48 238 3.17e-13 68.2
MS.gene27853.t1 MTR_3g070210 27.805 205 133 6 35 225 52 255 1.39e-12 66.6
MS.gene27853.t1 MTR_1g095070 28.022 182 113 4 81 247 25 203 4.02e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27853.t1 AT4G18910 74.400 250 57 1 26 268 40 289 4.44e-137 388
MS.gene27853.t1 AT4G19030 64.685 286 80 3 4 268 7 292 1.59e-133 379
MS.gene27853.t1 AT5G37810 59.917 242 97 0 28 269 33 274 4.82e-105 306
MS.gene27853.t1 AT5G37820 56.489 262 108 1 11 266 10 271 2.46e-103 302
MS.gene27853.t1 AT5G37820 56.489 262 108 1 11 266 46 307 1.30e-102 301
MS.gene27853.t1 AT2G34390 53.623 276 112 4 5 265 7 281 6.19e-99 291
MS.gene27853.t1 AT1G31885 50.558 269 122 2 2 259 3 271 7.05e-98 290
MS.gene27853.t1 AT4G18910 75.000 184 39 1 25 201 39 222 6.19e-94 276
MS.gene27853.t1 AT4G19030 63.761 218 58 3 4 200 7 224 1.46e-92 273
MS.gene27853.t1 AT2G34390 55.932 236 96 2 34 262 2 236 1.79e-92 273
MS.gene27853.t1 AT1G31885 55.760 217 89 1 50 259 1 217 1.96e-89 266
MS.gene27853.t1 AT4G19030 61.809 199 55 3 4 181 7 205 7.84e-81 243
MS.gene27853.t1 AT2G34390 55.189 212 79 4 5 201 7 217 2.46e-75 229
MS.gene27853.t1 AT1G80760 52.294 218 103 1 35 252 77 293 6.09e-73 226
MS.gene27853.t1 AT4G10380 50.216 231 108 2 35 259 75 304 9.09e-72 223
MS.gene27853.t1 AT2G34390 54.124 194 73 4 5 183 7 199 3.93e-67 208
MS.gene27853.t1 AT3G06100 38.356 219 133 2 37 254 45 262 1.17e-46 157
MS.gene27853.t1 AT3G06100 38.785 214 129 2 41 253 1 213 1.78e-46 155
MS.gene27853.t1 AT1G31885 47.863 117 57 1 2 114 3 119 9.15e-34 120
MS.gene27853.t1 AT2G29870 55.789 95 42 0 153 247 20 114 1.81e-32 116
MS.gene27853.t1 AT5G47450 33.191 235 147 3 33 257 14 248 9.55e-28 107
MS.gene27853.t1 AT3G16240 33.333 231 136 6 26 245 10 233 2.03e-27 106
MS.gene27853.t1 AT3G06100 35.915 142 89 2 114 254 41 181 6.36e-25 98.6
MS.gene27853.t1 AT4G17340 32.083 240 150 5 27 256 11 247 1.52e-24 99.0
MS.gene27853.t1 AT1G17810 30.116 259 165 5 16 260 4 260 1.86e-24 99.4
MS.gene27853.t1 AT3G26520 29.845 258 160 7 18 263 5 253 5.22e-22 92.4
MS.gene27853.t1 AT1G73190 28.400 250 165 4 16 253 4 251 3.17e-21 90.5
MS.gene27853.t1 AT2G36830 32.353 238 146 5 18 246 5 236 7.26e-21 89.4
MS.gene27853.t1 AT2G25810 31.878 229 144 8 32 253 13 236 1.15e-20 88.6
MS.gene27853.t1 AT4G35100 31.579 228 141 5 35 247 35 262 1.20e-20 89.4
MS.gene27853.t1 AT4G35100 31.579 228 141 5 35 247 35 262 1.20e-20 89.4
MS.gene27853.t1 AT2G16850 30.702 228 143 5 35 247 33 260 2.17e-20 88.6
MS.gene27853.t1 AT2G39010 29.362 235 143 6 35 247 35 268 9.52e-19 84.0
MS.gene27853.t1 AT2G45960 29.310 232 146 6 32 247 46 275 1.76e-18 83.2
MS.gene27853.t1 AT3G61430 28.821 229 145 6 35 247 49 275 2.21e-18 83.2
MS.gene27853.t1 AT3G61430 28.821 229 145 6 35 247 49 275 2.21e-18 83.2
MS.gene27853.t1 AT1G01620 28.139 231 145 5 35 247 49 276 6.45e-18 81.6
MS.gene27853.t1 AT3G47440 29.032 217 144 3 37 245 22 236 8.97e-18 80.9
MS.gene27853.t1 AT5G60660 25.746 268 161 6 15 247 5 269 1.29e-17 80.9
MS.gene27853.t1 AT4G00430 28.139 231 145 5 35 247 50 277 1.32e-17 80.9
MS.gene27853.t1 AT4G23400 27.706 231 146 5 35 247 50 277 2.72e-17 80.1
MS.gene27853.t1 AT3G54820 26.033 242 158 4 27 247 27 268 1.46e-16 78.2
MS.gene27853.t1 AT3G53420 25.714 245 155 5 27 247 28 269 3.29e-16 77.0
MS.gene27853.t1 AT3G53420 25.714 245 155 5 27 247 28 269 3.29e-16 77.0
MS.gene27853.t1 AT4G01470 29.046 241 159 4 15 247 1 237 3.40e-16 76.3
MS.gene27853.t1 AT2G45960 28.922 204 131 4 32 222 46 248 6.40e-15 73.2
MS.gene27853.t1 AT2G37170 25.306 245 156 5 27 247 69 310 7.68e-15 73.6
MS.gene27853.t1 AT2G37170 25.306 245 156 5 27 247 26 267 7.81e-15 73.2
MS.gene27853.t1 AT2G45960 28.922 204 131 4 32 222 46 248 8.06e-15 73.2
MS.gene27853.t1 AT1G01620 28.718 195 121 4 68 247 13 204 1.39e-14 71.2
MS.gene27853.t1 AT2G37180 25.306 245 156 5 27 247 26 267 3.70e-14 71.2

Find 64 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTTATGTTTATCATATC+TGG 0.173723 4.1:-13066068 MS.gene27853:CDS
TTCCTGGAATTTCAATTGTT+TGG 0.217869 4.1:-13066842 MS.gene27853:CDS
TCCTGGAATTTCAATTGTTT+GGG 0.246530 4.1:-13066841 MS.gene27853:CDS
TACTTGGTGTCTCCTATTCT+TGG 0.280405 4.1:-13065033 MS.gene27853:CDS
CTGTTTCAAGGGAAATCTTT+TGG 0.298217 4.1:+13066731 None:intergenic
GAAAATGTTGTAACACTTCC+TGG 0.313477 4.1:-13066858 MS.gene27853:CDS
ACATATTTCTTGATATTTGC+TGG 0.333245 4.1:-13066912 MS.gene27853:CDS
TACTCTCTTGGTCACATCTC+TGG 0.349138 4.1:-13066795 MS.gene27853:CDS
CTTCTGAATGTGATGTTTGC+AGG 0.351462 4.1:-13065630 MS.gene27853:CDS
GCAGGTCCTATACTTCTTGC+TGG 0.362295 4.1:+13065090 None:intergenic
ATGGTGCTGGTTTACTCTCT+TGG 0.376622 4.1:-13066807 MS.gene27853:CDS
CCCCAAACAATTGAAATTCC+AGG 0.378868 4.1:+13066840 None:intergenic
TTCATTGATGCGCCTGTGAT+TGG 0.379902 4.1:+13065114 None:intergenic
CACTGCTTCTTTCTTGCAGA+AGG 0.380717 4.1:-13067110 MS.gene27853:intron
GGCAAACATAATCAGTTTGT+TGG 0.384708 4.1:-13066149 MS.gene27853:CDS
TTGGAGCTGTGGCTGGTGCA+TGG 0.386493 4.1:-13065014 MS.gene27853:CDS
TACGTTTAAAACAATCTCAT+TGG 0.388236 4.1:+13067177 None:intergenic
CAAAAGATTTCCCTTGAAAC+AGG 0.391841 4.1:-13066730 MS.gene27853:intron
GGGACTTGCTGTGATGGTGC+TGG 0.409165 4.1:-13066820 MS.gene27853:CDS
ACTCTTAGACTAATATTTAA+CGG 0.412369 4.1:-13066170 MS.gene27853:CDS
CCTATTCTTGGAGCTGTGGC+TGG 0.421647 4.1:-13065021 MS.gene27853:CDS
TGGAGCTGTGGCTGGTGCAT+GGG 0.426749 4.1:-13065013 MS.gene27853:CDS
TTTGTTGGAACACTTCCAGC+TGG 0.433109 4.1:-13066134 MS.gene27853:CDS
GATATTTGCTGGTTGTGCTT+CGG 0.453067 4.1:-13066901 MS.gene27853:CDS
CCAGCCACAGCTCCAAGAAT+AGG 0.454788 4.1:+13065021 None:intergenic
GAGTTTGGTCACCTGTTTCA+AGG 0.455962 4.1:+13066719 None:intergenic
ACAGTGAATACAGAGGAATA+TGG 0.460120 4.1:-13065059 MS.gene27853:CDS
ATGAATCCAGCAAGAAGTAT+AGG 0.469904 4.1:-13065096 MS.gene27853:CDS
TGCTGGTTGTGCTTCGGTGT+TGG 0.473807 4.1:-13066895 MS.gene27853:CDS
ATTCAATCACAAAAGCTTGA+AGG 0.482940 4.1:+13066105 None:intergenic
TGCAGATTGGTGAGTTGGCT+GGG 0.483568 4.1:-13065674 MS.gene27853:intron
TGTTTGGGGACTTGCTGTGA+TGG 0.487027 4.1:-13066826 MS.gene27853:CDS
TTGCAGATTGGTGAGTTGGC+TGG 0.488849 4.1:-13065675 MS.gene27853:intron
CTCTTGGTGATCTCGCGAAC+CGG 0.489371 4.1:+13064961 None:intergenic
CATGGGTTTATAACGTCATT+CGG 0.494271 4.1:-13064996 MS.gene27853:CDS
CAGTTGGTAGCTGAGGTGAT+AGG 0.497515 4.1:-13066936 MS.gene27853:CDS
CAGAGGAATATGGATATACT+TGG 0.503606 4.1:-13065049 MS.gene27853:CDS
CTCCAGATATGATAAACATA+AGG 0.504080 4.1:+13066066 None:intergenic
AAACTTGCAGATTGGTGAGT+TGG 0.505757 4.1:-13065679 MS.gene27853:intron
GAAAGAAGCAGTGGCATGAG+AGG 0.513919 4.1:+13067120 None:intergenic
TATTCACTGTGTAGAAAAGC+AGG 0.516811 4.1:+13065072 None:intergenic
GCTTGAAGGTCAGACCCAGC+TGG 0.524734 4.1:+13066119 None:intergenic
TTGTTGGAACACTTCCAGCT+GGG 0.527294 4.1:-13066133 MS.gene27853:CDS
CCTAAAAGCAGCAAAACCAT+AGG 0.538587 4.1:-13064929 None:intergenic
CCTGGAATTTCAATTGTTTG+GGG 0.538744 4.1:-13066840 MS.gene27853:CDS
GAGTTGGCTGGGATTGCCGT+TGG 0.544203 4.1:-13065663 MS.gene27853:CDS
GTCTCCTATTCTTGGAGCTG+TGG 0.546485 4.1:-13065025 MS.gene27853:CDS
AAGAGTTCCACTTGCAAGTG+TGG 0.546686 4.1:+13066187 None:intergenic
CATTCGGTACACGGATAAGC+CGG 0.557498 4.1:-13064980 MS.gene27853:CDS
GCAAAAGCAATGGTAACTGC+AGG 0.558375 4.1:+13066759 None:intergenic
AGTTTGGTCACCTGTTTCAA+GGG 0.560701 4.1:+13066720 None:intergenic
ACTTGAGCTGCTACATAAGC+CGG 0.561215 4.1:+13066218 None:intergenic
ATTTATGCAGTTGGTAGCTG+AGG 0.567762 4.1:-13066943 MS.gene27853:intron
TTTGGATCCACACTTGCAAG+TGG 0.573697 4.1:-13066194 MS.gene27853:CDS
TTCTGAATGTGATGTTTGCA+GGG 0.577720 4.1:-13065629 MS.gene27853:intron
CTAAAAGCAGCAAAACCATA+GGG 0.583342 4.1:-13064928 None:intergenic
TTATAACGTCATTCGGTACA+CGG 0.586612 4.1:-13064989 MS.gene27853:CDS
TTATATGACAGACCAATCAC+AGG 0.603319 4.1:-13065126 MS.gene27853:intron
TTTGGTGCTAGCAAAAGCAA+TGG 0.603584 4.1:+13066749 None:intergenic
CAGAAGTACAGTAGATCCAA+CGG 0.645576 4.1:+13065647 None:intergenic
CGTTACCGCTCTATTATCAG+TGG 0.661375 4.1:+13066040 None:intergenic
CTTCTGCAAGAAAGAAGCAG+TGG 0.662046 4.1:+13067111 None:intergenic
TTTCTACACAGTGAATACAG+AGG 0.725695 4.1:-13065066 MS.gene27853:CDS
AGTTGCCACTGATAATAGAG+CGG 0.742875 4.1:-13066045 MS.gene27853:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATTTTTTTTTTTAAAA+TGG + chr4.1:13066716-13066735 None:intergenic 0.0%
!! TATTATAAACTAATATGAAA+AGG + chr4.1:13065554-13065573 None:intergenic 10.0%
!!! ATGATAATATTGAATAATTT+TGG + chr4.1:13066544-13066563 None:intergenic 10.0%
!!! TGATAATATTGAATAATTTT+GGG + chr4.1:13066543-13066562 None:intergenic 10.0%
!! AAATATGATTGATTATCATT+TGG + chr4.1:13066856-13066875 None:intergenic 15.0%
!! AGGGAATAAAATAAAAAATT+GGG - chr4.1:13065628-13065647 MS.gene27853:intron 15.0%
!! GAATTACAAATATTCAAATA+TGG + chr4.1:13066391-13066410 None:intergenic 15.0%
!! TAGGGAATAAAATAAAAAAT+TGG - chr4.1:13065627-13065646 MS.gene27853:intron 15.0%
!!! TTTGCTATTTGATTTATTAA+AGG - chr4.1:13065112-13065131 MS.gene27853:CDS 15.0%
!! AATAATTGACATGATTTCTT+TGG + chr4.1:13065578-13065597 None:intergenic 20.0%
!! AATCTTTCCATTTATTGTAT+AGG - chr4.1:13065892-13065911 MS.gene27853:intron 20.0%
!! ACTCTTAGACTAATATTTAA+CGG - chr4.1:13065965-13065984 MS.gene27853:intron 20.0%
!! ATAAAAGCCAATAAATAGAA+TGG + chr4.1:13066419-13066438 None:intergenic 20.0%
!! TCAAACTCTAATATAAAAAG+AGG - chr4.1:13066249-13066268 MS.gene27853:intron 20.0%
!! TGTGATTTAGTATTTATTCT+TGG - chr4.1:13066620-13066639 MS.gene27853:intron 20.0%
!!! AATCTGAAACTTTTTTTTCA+TGG - chr4.1:13065158-13065177 MS.gene27853:intron 20.0%
!!! TAATTTCATCTCTCTTATTT+TGG - chr4.1:13066169-13066188 MS.gene27853:CDS 20.0%
! AACACACAATTTCATTGAAT+AGG - chr4.1:13065608-13065627 MS.gene27853:intron 25.0%
! AATACGTGTTTAGCAAAATA+GGG - chr4.1:13066210-13066229 MS.gene27853:CDS 25.0%
! ACACACAATTTCATTGAATA+GGG - chr4.1:13065609-13065628 MS.gene27853:intron 25.0%
! ACATATTTCTTGATATTTGC+TGG - chr4.1:13065223-13065242 MS.gene27853:intron 25.0%
! AGATTTATAGATAGTTAGCA+AGG + chr4.1:13065877-13065896 None:intergenic 25.0%
! ATACTATATGTTTCTCTTCA+TGG - chr4.1:13066674-13066693 MS.gene27853:intron 25.0%
! TAATACGTGTTTAGCAAAAT+AGG - chr4.1:13066209-13066228 MS.gene27853:CDS 25.0%
! TACCTTATGTTTATCATATC+TGG - chr4.1:13066067-13066086 MS.gene27853:CDS 25.0%
! TCTTAGACCATTCTATTTAT+TGG - chr4.1:13066409-13066428 MS.gene27853:intron 25.0%
! TTTGCCAAATAGTTGAAAAA+TGG + chr4.1:13065443-13065462 None:intergenic 25.0%
!! CTCAACCAGATTTTAAATAA+AGG - chr4.1:13065702-13065721 MS.gene27853:intron 25.0%
!! GCAAACCTTTATTTAAAATC+TGG + chr4.1:13065710-13065729 None:intergenic 25.0%
!! TACGTTTAAAACAATCTCAT+TGG + chr4.1:13064961-13064980 None:intergenic 25.0%
!! TTATTTTCAAACTTGCAGAT+TGG - chr4.1:13066448-13066467 MS.gene27853:intron 25.0%
!! TTGTTTCTTGCTTGTTAATT+TGG - chr4.1:13066138-13066157 MS.gene27853:CDS 25.0%
!!! AAAACTTGTTAGGAAATTAG+AGG - chr4.1:13065460-13065479 MS.gene27853:intron 25.0%
!!! ATTCATTTTTCAAGCAAAGT+AGG + chr4.1:13065509-13065528 None:intergenic 25.0%
!!! GATTGTTTTAAACGTAAACA+AGG - chr4.1:13064965-13064984 MS.gene27853:CDS 25.0%
!!! TTGTAACGTTTTGAATCATT+TGG - chr4.1:13066306-13066325 MS.gene27853:intron 25.0%
ACTGTTAAATACGATCAATG+CGG + chr4.1:13065802-13065821 None:intergenic 30.0%
ATACGTGTTTAGCAAAATAG+GGG - chr4.1:13066211-13066230 MS.gene27853:CDS 30.0%
ATTCAATCACAAAAGCTTGA+AGG + chr4.1:13066033-13066052 None:intergenic 30.0%
ATTCTCCATAATGTCAAGAA+CGG - chr4.1:13065823-13065842 MS.gene27853:intron 30.0%
CTCCAGATATGATAAACATA+AGG + chr4.1:13066072-13066091 None:intergenic 30.0%
TAGTTGAAAAATGGTGAGTT+TGG + chr4.1:13065434-13065453 None:intergenic 30.0%
TCCATTTATTGTATAGGTAC+CGG - chr4.1:13065898-13065917 MS.gene27853:intron 30.0%
TCCTGGAATTTCAATTGTTT+GGG - chr4.1:13065294-13065313 MS.gene27853:intron 30.0%
TGTGACAATATTTATGCAGT+TGG - chr4.1:13065183-13065202 MS.gene27853:intron 30.0%
TTCCTGGAATTTCAATTGTT+TGG - chr4.1:13065293-13065312 MS.gene27853:intron 30.0%
TTGAAATTTGCAGAATCATG+AGG + chr4.1:13066976-13066995 None:intergenic 30.0%
TTGAGACAAAAAGAACGAAA+CGG + chr4.1:13065056-13065075 None:intergenic 30.0%
! ACTATTTGGCAAAACTTGTT+AGG - chr4.1:13065450-13065469 MS.gene27853:intron 30.0%
!! CTCACCATTTTTCAACTATT+TGG - chr4.1:13065436-13065455 MS.gene27853:intron 30.0%
!!! GTTAGCAAGGTTTTAAATAG+AGG + chr4.1:13065864-13065883 None:intergenic 30.0%
ACAGTGAATACAGAGGAATA+TGG - chr4.1:13067076-13067095 MS.gene27853:intron 35.0%
CAAAAGATTTCCCTTGAAAC+AGG - chr4.1:13065405-13065424 MS.gene27853:intron 35.0%
CAGAGGAATATGGATATACT+TGG - chr4.1:13067086-13067105 MS.gene27853:intron 35.0%
CATGGGTTTATAACGTCATT+CGG - chr4.1:13067139-13067158 MS.gene27853:CDS 35.0%
CCTGGAATTTCAATTGTTTG+GGG - chr4.1:13065295-13065314 MS.gene27853:intron 35.0%
GAAAATGTTGTAACACTTCC+TGG - chr4.1:13065277-13065296 MS.gene27853:intron 35.0%
GGCAAACATAATCAGTTTGT+TGG - chr4.1:13065986-13066005 MS.gene27853:intron 35.0%
TATTCACTGTGTAGAAAAGC+AGG + chr4.1:13067066-13067085 None:intergenic 35.0%
TTATAACGTCATTCGGTACA+CGG - chr4.1:13067146-13067165 MS.gene27853:CDS 35.0%
TTATATGACAGACCAATCAC+AGG - chr4.1:13067009-13067028 MS.gene27853:intron 35.0%
TTCTGAATGTGATGTTTGCA+GGG - chr4.1:13066506-13066525 MS.gene27853:intron 35.0%
TTTCTACACAGTGAATACAG+AGG - chr4.1:13067069-13067088 MS.gene27853:intron 35.0%
! ATGAATCCAGCAAGAAGTAT+AGG - chr4.1:13067039-13067058 MS.gene27853:intron 35.0%
! CAAGCAAGAAACAAAGCATT+AGG + chr4.1:13066132-13066151 None:intergenic 35.0%
! CTGTTTCAAGGGAAATCTTT+TGG + chr4.1:13065407-13065426 None:intergenic 35.0%
! TATGTAGCAGCTCAAGTTTT+TGG - chr4.1:13065923-13065942 MS.gene27853:intron 35.0%
! TTACGTAGTTCATCACCTTT+TGG - chr4.1:13066568-13066587 MS.gene27853:intron 35.0%
!! ACTTGCTATCTGTTTCCAAA+AGG + chr4.1:13066586-13066605 None:intergenic 35.0%
!! TTTTAGGAAAGATGAGCTCT+TGG + chr4.1:13067193-13067212 None:intergenic 35.0%
AAACTTGCAGATTGGTGAGT+TGG - chr4.1:13066456-13066475 MS.gene27853:intron 40.0%
AGTTGCCACTGATAATAGAG+CGG - chr4.1:13066090-13066109 MS.gene27853:CDS 40.0%
AGTTTGGTCACCTGTTTCAA+GGG + chr4.1:13065418-13065437 None:intergenic 40.0%
CCCCAAACAATTGAAATTCC+AGG + chr4.1:13065298-13065317 None:intergenic 40.0%
CTGCAACATGATTGCAATTG+AGG - chr4.1:13065776-13065795 MS.gene27853:intron 40.0%
CTTCTGAATGTGATGTTTGC+AGG - chr4.1:13066505-13066524 MS.gene27853:intron 40.0%
GCCGGTACCTATACAATAAA+TGG + chr4.1:13065902-13065921 None:intergenic 40.0%
GTGTTTAGCAAAATAGGGGT+TGG - chr4.1:13066215-13066234 MS.gene27853:CDS 40.0%
! CAGAAGTACAGTAGATCCAA+CGG + chr4.1:13066491-13066510 None:intergenic 40.0%
! GATATTTGCTGGTTGTGCTT+CGG - chr4.1:13065234-13065253 MS.gene27853:intron 40.0%
!! ATTTATGCAGTTGGTAGCTG+AGG - chr4.1:13065192-13065211 MS.gene27853:intron 40.0%
!! TACTTGGTGTCTCCTATTCT+TGG - chr4.1:13067102-13067121 MS.gene27853:intron 40.0%
!! TTTGGTGCTAGCAAAAGCAA+TGG + chr4.1:13065389-13065408 None:intergenic 40.0%
AAGAGTTCCACTTGCAAGTG+TGG + chr4.1:13065951-13065970 None:intergenic 45.0%
AAGGTTTGCGATCGTGATCT+AGG - chr4.1:13065721-13065740 MS.gene27853:intron 45.0%
ACTTGAGCTGCTACATAAGC+CGG + chr4.1:13065920-13065939 None:intergenic 45.0%
CACTGCTTCTTTCTTGCAGA+AGG - chr4.1:13065025-13065044 MS.gene27853:CDS 45.0%
CGTTACCGCTCTATTATCAG+TGG + chr4.1:13066098-13066117 None:intergenic 45.0%
CTTCTGCAAGAAAGAAGCAG+TGG + chr4.1:13065027-13065046 None:intergenic 45.0%
GAGTTTGGTCACCTGTTTCA+AGG + chr4.1:13065419-13065438 None:intergenic 45.0%
GCAAAAGCAATGGTAACTGC+AGG + chr4.1:13065379-13065398 None:intergenic 45.0%
TACTCTCTTGGTCACATCTC+TGG - chr4.1:13065340-13065359 MS.gene27853:intron 45.0%
TTCATTGATGCGCCTGTGAT+TGG + chr4.1:13067024-13067043 None:intergenic 45.0%
TTGTTGGAACACTTCCAGCT+GGG - chr4.1:13066002-13066021 MS.gene27853:intron 45.0%
TTTGGATCCACACTTGCAAG+TGG - chr4.1:13065941-13065960 MS.gene27853:intron 45.0%
TTTGTTGGAACACTTCCAGC+TGG - chr4.1:13066001-13066020 MS.gene27853:intron 45.0%
! GAGTGCCGTTCTTGACATTA+TGG + chr4.1:13065831-13065850 None:intergenic 45.0%
!! ATGGTGCTGGTTTACTCTCT+TGG - chr4.1:13065328-13065347 MS.gene27853:intron 45.0%
CATTCGGTACACGGATAAGC+CGG - chr4.1:13067155-13067174 MS.gene27853:CDS 50.0%
GAAAGAAGCAGTGGCATGAG+AGG + chr4.1:13065018-13065037 None:intergenic 50.0%
GCAGGTCCTATACTTCTTGC+TGG + chr4.1:13067048-13067067 None:intergenic 50.0%
GTCTCCTATTCTTGGAGCTG+TGG - chr4.1:13067110-13067129 MS.gene27853:intron 50.0%
! TGCAGATTGGTGAGTTGGCT+GGG - chr4.1:13066461-13066480 MS.gene27853:intron 50.0%
! TTGCAGATTGGTGAGTTGGC+TGG - chr4.1:13066460-13066479 MS.gene27853:intron 50.0%
!! CAGTTGGTAGCTGAGGTGAT+AGG - chr4.1:13065199-13065218 MS.gene27853:intron 50.0%
!! TGTTTGGGGACTTGCTGTGA+TGG - chr4.1:13065309-13065328 MS.gene27853:intron 50.0%
CCAGCCACAGCTCCAAGAAT+AGG + chr4.1:13067117-13067136 None:intergenic 55.0%
CCTATTCTTGGAGCTGTGGC+TGG - chr4.1:13067114-13067133 MS.gene27853:CDS 55.0%
! CTCTTGGTGATCTCGCGAAC+CGG + chr4.1:13067177-13067196 None:intergenic 55.0%
! TGCTGGTTGTGCTTCGGTGT+TGG - chr4.1:13065240-13065259 MS.gene27853:intron 55.0%
GAGTTGGCTGGGATTGCCGT+TGG - chr4.1:13066472-13066491 MS.gene27853:intron 60.0%
GCTTGAAGGTCAGACCCAGC+TGG + chr4.1:13066019-13066038 None:intergenic 60.0%
TGGAGCTGTGGCTGGTGCAT+GGG - chr4.1:13067122-13067141 MS.gene27853:CDS 60.0%
TTGGAGCTGTGGCTGGTGCA+TGG - chr4.1:13067121-13067140 MS.gene27853:CDS 60.0%
!! GGGACTTGCTGTGATGGTGC+TGG - chr4.1:13065315-13065334 MS.gene27853:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 13064930 13067227 13064930 ID=MS.gene27853
chr4.1 mRNA 13064930 13067227 13064930 ID=MS.gene27853.t1;Parent=MS.gene27853
chr4.1 exon 13067111 13067227 13067111 ID=MS.gene27853.t1.exon1;Parent=MS.gene27853.t1
chr4.1 CDS 13067111 13067227 13067111 ID=cds.MS.gene27853.t1;Parent=MS.gene27853.t1
chr4.1 exon 13066731 13066955 13066731 ID=MS.gene27853.t1.exon2;Parent=MS.gene27853.t1
chr4.1 CDS 13066731 13066955 13066731 ID=cds.MS.gene27853.t1;Parent=MS.gene27853.t1
chr4.1 exon 13066046 13066243 13066046 ID=MS.gene27853.t1.exon3;Parent=MS.gene27853.t1
chr4.1 CDS 13066046 13066243 13066046 ID=cds.MS.gene27853.t1;Parent=MS.gene27853.t1
chr4.1 exon 13065630 13065691 13065630 ID=MS.gene27853.t1.exon4;Parent=MS.gene27853.t1
chr4.1 CDS 13065630 13065691 13065630 ID=cds.MS.gene27853.t1;Parent=MS.gene27853.t1
chr4.1 exon 13064930 13065137 13064930 ID=MS.gene27853.t1.exon5;Parent=MS.gene27853.t1
chr4.1 CDS 13064930 13065137 13064930 ID=cds.MS.gene27853.t1;Parent=MS.gene27853.t1
Gene Sequence

>MS.gene27853

ATGGAAGAAAATTCAGCTAGCAATGCAACCAATGAGATTGTTTTAAACGTAAACAAGGCTGTCTCTAACACAAGTGAAGACTCAACCTCTCATGCCACTGCTTCTTTCTTGCAGAAGGTGCATCCGTTTCGTTCTTTTTGTCTCAAAAACAAGTTCAAAATGTTTTGTTTGTTCATAACTAATTTGCTATTTGATTTATTAAAGGTTTTGTTATGTTATGTCTGCTAAAATCTGAAACTTTTTTTTCATGGATTGTGACAATATTTATGCAGTTGGTAGCTGAGGTGATAGGCACATATTTCTTGATATTTGCTGGTTGTGCTTCGGTGTTGGTGAACAAGAACAATGAAAATGTTGTAACACTTCCTGGAATTTCAATTGTTTGGGGACTTGCTGTGATGGTGCTGGTTTACTCTCTTGGTCACATCTCTGGTGCTCATTTCAATCCTGCAGTTACCATTGCTTTTGCTAGCACCAAAAGATTTCCCTTGAAACAGGTGACCAAACTCACCATTTTTCAACTATTTGGCAAAACTTGTTAGGAAATTAGAGGTCAAATTAGTTCATGATCTACAGCCTACTTTGCTTGAAAAATGAATTTCTTTTTCTTACTTATTTTGACCTTTTCATATTAGTTTATAATATCCAAAGAAATCATGTCAATTATTGCACATTGTGAACACACAATTTCATTGAATAGGGAATAAAATAAAAAATTGGGTGTTCACACACACGTAGATAGAAAAATATTGTAATGAAAGATAACTTATCTCTCAACCAGATTTTAAATAAAGGTTTGCGATCGTGATCTAGGTAGCGACATAATAATTTTTTATGTTGTCGACACTGCAACATGATTGCAATTGAGGCCGCATTGATCGTATTTAACAGTGATTCTCCATAATGTCAAGAACGGCACTCGTCACTGCGACCTCTATTTAAAACCTTGCTAACTATCTATAAATCTTTCCATTTATTGTATAGGTACCGGCTTATGTAGCAGCTCAAGTTTTTGGATCCACACTTGCAAGTGGAACTCTTAGACTAATATTTAACGGCAAACATAATCAGTTTGTTGGAACACTTCCAGCTGGGTCTGACCTTCAAGCTTTTGTGATTGAATTCATCATCACTTTTTACCTTATGTTTATCATATCTGGAGTTGCCACTGATAATAGAGCGGTAACGTGTTTGTTCATCCTAATGCTTTGTTTCTTGCTTGTTAATTTGGTTATTAACTAATTTCATCTCTCTTATTTTGGTGTTAAGTATTTTGATTTAATACGTGTTTAGCAAAATAGGGGTTGGCTAATCATCTTTCAAACTCTAATATAAAAAGAGGCAATTAAATTGACTAGTTGTCTTAATATATATAGTTGTAACGTTTTGAATCATTTGGAAGAATGCTAGCAGCTAGTAGTTTACATAACAAGACAAAGCTTATACGAAACAAAACGACCATATTTGAATATTTGTAATTCTTAGACCATTCTATTTATTGGCTTTTATTTATTCGTTTTATTTTCAAACTTGCAGATTGGTGAGTTGGCTGGGATTGCCGTTGGATCTACTGTACTTCTGAATGTGATGTTTGCAGGGTATTAACTTATCCCAAAATTATTCAATATTATCATTTGCTTACGTAGTTCATCACCTTTTGGAAACAGATAGCAAGTCATGATCTCATTTGTGTGATTTAGTATTTATTCTTGGAGCATACTATATGTTTCTTTTTTTTTGAAGAATACTATATGTTTCTCTTCATGGTTAAGATCATGTGATGCCATTTTAAAAAAAAAAATAATAATTATTAATAAATCATGTGATGCAGTAAAAACTTAGAATAGAACACTGTATTATGTTACTTGCTGAGTTTAATTTTGATACACTTTTAGTGTAAAATGTTTTATTCTATCAGTGTCACCAAATGATAATCAATCATATTTTTTACTTTAGAAACTTACGATAAAAATCAAATGCTTTTAATAATCTAACGATTATGATTGATCAACAATTTATTCTAAAAGTGTATATGAAGTAAATCCTCATGATTCTGCAAATTTCAAATGACATTATTTCTTATATGACAGACCAATCACAGGCGCATCAATGAATCCAGCAAGAAGTATAGGACCTGCTTTTCTACACAGTGAATACAGAGGAATATGGATATACTTGGTGTCTCCTATTCTTGGAGCTGTGGCTGGTGCATGGGTTTATAACGTCATTCGGTACACGGATAAGCCGGTTCGCGAGATCACCAAGAGCTCATCTTTCCTAAAAGCAGCAAAACCATAG
Protein sequence

>MS.gene27853.t1

MEENSASNATNEIVLNVNKAVSNTSEDSTSHATASFLQKLVAEVIGTYFLIFAGCASVLVNKNNENVVTLPGISIVWGLAVMVLVYSLGHISGAHFNPAVTIAFASTKRFPLKQVPAYVAAQVFGSTLASGTLRLIFNGKHNQFVGTLPAGSDLQAFVIEFIITFYLMFIISGVATDNRAIGELAGIAVGSTVLLNVMFAGPITGASMNPARSIGPAFLHSEYRGIWIYLVSPILGAVAGAWVYNVIRYTDKPVREITKSSSFLKAAKP