AlfalfaGEDB | MS.gene065973

M. sativa cultivar XinJiangDaYe / MS.gene065973

KEGG: K09874
GO: GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0015267 GO:0016020 GO:0022803 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0110165
PFAM: PF00230.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065973.t1	XP_003629863.1	98.1	269	5	0	1	269	1	269	6.30E-139	503.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065973.t1	P08995	68.7	265	83	0	1	265	1	265	2.8e-103	376.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065973.t1	G7L7B6	98.1	269	5	0	1	269	1	269	4.6e-139	503.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04921	MS.gene065973	0.838743	2.40E-57	-1.69E-46
MS.gene050170	MS.gene065973	-0.837983	3.77E-57	-1.69E-46
MS.gene050496	MS.gene065973	-0.821863	3.22E-53	-1.69E-46
MS.gene050868	MS.gene065973	-0.806593	7.63E-50	-1.69E-46
MS.gene050871	MS.gene065973	-0.804376	2.23E-49	-1.69E-46
MS.gene051044	MS.gene065973	0.81437	1.60E-51	-1.69E-46
MS.gene051138	MS.gene065973	0.811753	5.98E-51	-1.69E-46
MS.gene051440	MS.gene065973	0.826968	2.03E-54	-1.69E-46
MS.gene051651	MS.gene065973	0.803889	2.81E-49	-1.69E-46
MS.gene052494	MS.gene065973	0.839785	1.29E-57	-1.69E-46
MS.gene052606	MS.gene065973	0.824448	8.03E-54	-1.69E-46
MS.gene053097	MS.gene065973	0.844121	9.20E-59	-1.69E-46
MS.gene05470	MS.gene065973	-0.817018	4.10E-52	-1.69E-46
MS.gene055251	MS.gene065973	0.821817	3.30E-53	-1.69E-46
MS.gene05589	MS.gene065973	-0.840571	8.03E-58	-1.69E-46
MS.gene055942	MS.gene065973	0.803312	3.70E-49	-1.69E-46
MS.gene056198	MS.gene065973	0.811473	6.88E-51	-1.69E-46
MS.gene056504	MS.gene065973	-0.823033	1.72E-53	-1.69E-46
MS.gene056694	MS.gene065973	0.837605	4.71E-57	-1.69E-46
MS.gene056856	MS.gene065973	0.815326	9.79E-52	-1.69E-46
MS.gene057014	MS.gene065973	0.80536	1.39E-49	-1.69E-46
MS.gene057720	MS.gene065973	0.809706	1.66E-50	-1.69E-46
MS.gene057768	MS.gene065973	0.84201	3.36E-58	-1.69E-46
MS.gene058068	MS.gene065973	0.803029	4.24E-49	-1.69E-46
MS.gene058171	MS.gene065973	0.816962	4.22E-52	-1.69E-46
MS.gene058173	MS.gene065973	0.812002	5.28E-51	-1.69E-46
MS.gene058175	MS.gene065973	0.817659	2.94E-52	-1.69E-46
MS.gene058334	MS.gene065973	0.822548	2.23E-53	-1.69E-46
MS.gene058381	MS.gene065973	0.804925	1.71E-49	-1.69E-46
MS.gene059865	MS.gene065973	-0.805421	1.35E-49	-1.69E-46
MS.gene06000	MS.gene065973	-0.800609	1.33E-48	-1.69E-46
MS.gene060076	MS.gene065973	0.809898	1.51E-50	-1.69E-46
MS.gene060358	MS.gene065973	0.809955	1.47E-50	-1.69E-46
MS.gene060593	MS.gene065973	0.816334	5.83E-52	-1.69E-46
MS.gene060595	MS.gene065973	0.807152	5.81E-50	-1.69E-46
MS.gene060982	MS.gene065973	-0.820313	7.33E-53	-1.69E-46
MS.gene061038	MS.gene065973	0.821039	4.99E-53	-1.69E-46
MS.gene061044	MS.gene065973	0.874985	4.40E-68	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene065973	MS.gene79774	PPI
MS.gene021394	MS.gene065973	PPI
MS.gene067014	MS.gene065973	PPI
MS.gene059379	MS.gene065973	PPI
MS.gene002640	MS.gene065973	PPI
MS.gene065973	MS.gene037437	PPI
MS.gene065973	MS.gene002643	PPI
MS.gene004322	MS.gene065973	PPI
MS.gene89899	MS.gene065973	PPI
MS.gene065973	MS.gene004324	PPI
MS.gene065973	MS.gene004088	PPI
MS.gene27853	MS.gene065973	PPI
MS.gene065973	MS.gene023621	PPI
MS.gene004953	MS.gene065973	PPI
MS.gene036142	MS.gene065973	PPI
MS.gene025056	MS.gene065973	PPI
MS.gene065973	MS.gene49332	PPI
MS.gene062190	MS.gene065973	PPI
MS.gene025449	MS.gene065973	PPI
MS.gene065973	MS.gene042849	PPI
MS.gene065972	MS.gene065973	PPI
MS.gene065973	MS.gene002639	PPI
MS.gene065973	MS.gene004321	PPI
MS.gene065973	MS.gene006961	PPI
MS.gene61127	MS.gene065973	PPI
MS.gene32446	MS.gene065973	PPI
MS.gene72127	MS.gene065973	PPI
MS.gene31709	MS.gene065973	PPI
MS.gene21620	MS.gene065973	PPI
MS.gene71137	MS.gene065973	PPI
MS.gene34830	MS.gene065973	PPI
MS.gene019314	MS.gene065973	PPI
MS.gene27852	MS.gene065973	PPI
MS.gene006960	MS.gene065973	PPI
MS.gene72129	MS.gene065973	PPI
MS.gene065973	MS.gene01624	PPI
MS.gene021393	MS.gene065973	PPI
MS.gene065973	MS.gene03121	PPI
MS.gene065973	MS.gene93201	PPI
MS.gene03094	MS.gene065973	PPI
MS.gene39895	MS.gene065973	PPI
MS.gene07481	MS.gene065973	PPI
MS.gene01627	MS.gene065973	PPI
MS.gene32445	MS.gene065973	PPI
MS.gene006959	MS.gene065973	PPI
MS.gene072777	MS.gene065973	PPI
MS.gene03093	MS.gene065973	PPI
MS.gene024637	MS.gene065973	PPI
MS.gene065973	MS.gene048817	PPI
MS.gene065973	MS.gene006958	PPI
MS.gene065973	MS.gene01625	PPI
MS.gene065973	MS.gene006957	PPI
MS.gene021061	MS.gene065973	PPI
MS.gene065973	MS.gene67362	PPI
MS.gene83316	MS.gene065973	PPI
MS.gene065973	MS.gene03090	PPI
MS.gene069812	MS.gene065973	PPI
MS.gene065973	MS.gene052743	PPI
MS.gene065973	MS.gene059531	PPI
MS.gene065973	MS.gene33486	PPI
MS.gene059378	MS.gene065973	PPI
MS.gene065973	MS.gene059380	PPI
MS.gene065973	MS.gene023622	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065973.t1	MTR_8g087720	98.141	269	5	0	1	269	1	269	0.0	530
MS.gene065973.t1	MTR_2g017570	76.119	268	64	0	1	268	1	268	5.73e-151	422
MS.gene065973.t1	MTR_2g017610	76.119	268	64	0	1	268	1	268	6.26e-151	422
MS.gene065973.t1	MTR_8g087710	73.585	265	70	0	3	267	4	268	1.10e-150	422
MS.gene065973.t1	MTR_2g017590	75.769	260	63	0	7	266	5	264	1.34e-147	414
MS.gene065973.t1	MTR_2g017620	72.075	265	74	0	1	265	1	265	3.66e-140	395
MS.gene065973.t1	MTR_8g087720	98.010	201	4	0	1	201	1	201	1.15e-139	391
MS.gene065973.t1	MTR_2g017620	74.104	251	65	0	15	265	1	251	1.17e-136	385
MS.gene065973.t1	MTR_2g017660	64.364	275	91	2	1	268	1	275	1.11e-132	376
MS.gene065973.t1	MTR_8g087720	96.196	184	7	0	1	184	1	184	5.95e-125	353
MS.gene065973.t1	MTR_4g084880	62.931	232	84	1	37	266	34	265	8.41e-109	316
MS.gene065973.t1	MTR_2g017620	79.348	184	38	0	82	265	1	184	3.21e-106	306
MS.gene065973.t1	MTR_5g079570	54.867	226	101	1	28	253	30	254	3.08e-87	261
MS.gene065973.t1	MTR_3g008920	53.279	244	111	2	28	269	16	258	1.37e-86	259
MS.gene065973.t1	MTR_7g010650	49.402	251	125	2	1	251	1	249	1.82e-79	241
MS.gene065973.t1	MTR_4g006730	53.778	225	103	1	38	262	75	298	1.29e-76	235
MS.gene065973.t1	MTR_2g104940	51.111	225	109	1	38	262	80	303	2.98e-75	231
MS.gene065973.t1	MTR_4g006630	53.774	212	97	1	51	262	1	211	8.60e-73	222
MS.gene065973.t1	MTR_4g006730	53.302	212	98	1	51	262	1	211	3.95e-72	220
MS.gene065973.t1	MTR_1g097840	49.356	233	107	3	35	259	71	300	4.98e-69	215
MS.gene065973.t1	MTR_5g063930	45.349	258	124	5	4	255	8	254	3.13e-67	209
MS.gene065973.t1	MTR_4g006730	55.488	164	72	1	38	201	75	237	4.06e-51	168
MS.gene065973.t1	MTR_1g070385	38.073	218	134	1	35	252	59	275	4.03e-46	165
MS.gene065973.t1	MTR_1g097840	50.000	172	79	2	33	201	69	236	9.83e-46	154
MS.gene065973.t1	MTR_7g010650	44.199	181	99	2	1	181	1	179	2.95e-44	149
MS.gene065973.t1	MTR_4g006650	50.000	144	58	3	39	180	5	136	3.70e-32	120
MS.gene065973.t1	MTR_8g013680	31.915	235	149	4	26	252	10	241	1.69e-25	101
MS.gene065973.t1	MTR_2g101370	32.653	245	152	4	26	263	11	249	2.62e-25	100
MS.gene065973.t1	MTR_1g077540	31.624	234	151	4	27	253	12	243	9.35e-25	99.8
MS.gene065973.t1	MTR_5g012810	32.787	244	150	6	23	256	7	246	5.26e-23	94.7
MS.gene065973.t1	MTR_1g006490	32.093	215	137	6	37	245	18	229	3.74e-22	92.4
MS.gene065973.t1	MTR_1g006590	31.034	232	150	6	19	245	3	229	4.65e-21	89.7
MS.gene065973.t1	MTR_6g069040	29.461	241	147	6	27	253	12	243	7.83e-21	89.0
MS.gene065973.t1	MTR_3g118010	30.303	231	140	5	35	247	34	261	4.33e-19	84.7
MS.gene065973.t1	MTR_7g101190	28.879	232	144	5	27	237	28	259	1.15e-18	84.0
MS.gene065973.t1	MTR_4g063090	31.416	226	143	4	43	256	26	251	1.55e-18	82.8
MS.gene065973.t1	MTR_7g103030	30.802	237	143	7	23	247	9	236	8.80e-18	80.9
MS.gene065973.t1	MTR_1g115405	32.068	237	147	7	37	263	20	252	9.36e-18	80.9
MS.gene065973.t1	MTR_2g094270	28.821	229	145	5	32	247	48	271	6.11e-17	79.0
MS.gene065973.t1	MTR_5g010150	26.201	229	153	6	35	247	49	277	1.27e-16	78.2
MS.gene065973.t1	MTR_4g415300	27.193	228	151	6	35	247	53	280	1.30e-16	78.2
MS.gene065973.t1	MTR_8g098375	27.500	240	157	7	35	258	49	287	1.97e-16	77.4
MS.gene065973.t1	MTR_3g070210	28.261	230	147	7	35	247	52	280	2.19e-16	77.4
MS.gene065973.t1	MTR_5g082070	28.448	232	143	8	35	247	52	279	2.61e-16	77.4
MS.gene065973.t1	MTR_2g060360	28.761	226	147	5	37	252	18	239	8.21e-16	75.5
MS.gene065973.t1	MTR_4g059390	27.426	237	145	6	35	247	36	269	6.31e-15	73.2
MS.gene065973.t1	MTR_7g023350	27.155	232	162	2	37	261	20	251	2.68e-13	68.2
MS.gene065973.t1	MTR_2g094270	29.082	196	124	4	32	217	48	238	3.17e-13	68.2
MS.gene065973.t1	MTR_3g070210	27.805	205	133	6	35	225	52	255	1.39e-12	66.6
MS.gene065973.t1	MTR_1g095070	28.022	182	113	4	81	247	25	203	4.02e-12	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065973.t1	AT4G18910	74.400	250	57	1	26	268	40	289	4.44e-137	388
MS.gene065973.t1	AT4G19030	64.685	286	80	3	4	268	7	292	1.59e-133	379
MS.gene065973.t1	AT5G37810	59.917	242	97	0	28	269	33	274	4.82e-105	306
MS.gene065973.t1	AT5G37820	56.489	262	108	1	11	266	10	271	2.46e-103	302
MS.gene065973.t1	AT5G37820	56.489	262	108	1	11	266	46	307	1.30e-102	301
MS.gene065973.t1	AT2G34390	53.623	276	112	4	5	265	7	281	6.19e-99	291
MS.gene065973.t1	AT1G31885	50.558	269	122	2	2	259	3	271	7.05e-98	290
MS.gene065973.t1	AT4G18910	75.000	184	39	1	25	201	39	222	6.19e-94	276
MS.gene065973.t1	AT4G19030	63.761	218	58	3	4	200	7	224	1.46e-92	273
MS.gene065973.t1	AT2G34390	55.932	236	96	2	34	262	2	236	1.79e-92	273
MS.gene065973.t1	AT1G31885	55.760	217	89	1	50	259	1	217	1.96e-89	266
MS.gene065973.t1	AT4G19030	61.809	199	55	3	4	181	7	205	7.84e-81	243
MS.gene065973.t1	AT2G34390	55.189	212	79	4	5	201	7	217	2.46e-75	229
MS.gene065973.t1	AT1G80760	52.294	218	103	1	35	252	77	293	6.09e-73	226
MS.gene065973.t1	AT4G10380	50.216	231	108	2	35	259	75	304	9.09e-72	223
MS.gene065973.t1	AT2G34390	54.124	194	73	4	5	183	7	199	3.93e-67	208
MS.gene065973.t1	AT3G06100	38.356	219	133	2	37	254	45	262	1.17e-46	157
MS.gene065973.t1	AT3G06100	38.785	214	129	2	41	253	1	213	1.78e-46	155
MS.gene065973.t1	AT1G31885	47.863	117	57	1	2	114	3	119	9.15e-34	120
MS.gene065973.t1	AT2G29870	55.789	95	42	0	153	247	20	114	1.81e-32	116
MS.gene065973.t1	AT5G47450	33.191	235	147	3	33	257	14	248	9.55e-28	107
MS.gene065973.t1	AT3G16240	33.333	231	136	6	26	245	10	233	2.03e-27	106
MS.gene065973.t1	AT3G06100	35.915	142	89	2	114	254	41	181	6.36e-25	98.6
MS.gene065973.t1	AT4G17340	32.083	240	150	5	27	256	11	247	1.52e-24	99.0
MS.gene065973.t1	AT1G17810	30.116	259	165	5	16	260	4	260	1.86e-24	99.4
MS.gene065973.t1	AT3G26520	29.845	258	160	7	18	263	5	253	5.22e-22	92.4
MS.gene065973.t1	AT1G73190	28.400	250	165	4	16	253	4	251	3.17e-21	90.5
MS.gene065973.t1	AT2G36830	32.353	238	146	5	18	246	5	236	7.26e-21	89.4
MS.gene065973.t1	AT2G25810	31.878	229	144	8	32	253	13	236	1.15e-20	88.6
MS.gene065973.t1	AT4G35100	31.579	228	141	5	35	247	35	262	1.20e-20	89.4
MS.gene065973.t1	AT4G35100	31.579	228	141	5	35	247	35	262	1.20e-20	89.4
MS.gene065973.t1	AT2G16850	30.702	228	143	5	35	247	33	260	2.17e-20	88.6
MS.gene065973.t1	AT2G39010	29.362	235	143	6	35	247	35	268	9.52e-19	84.0
MS.gene065973.t1	AT2G45960	29.310	232	146	6	32	247	46	275	1.76e-18	83.2
MS.gene065973.t1	AT3G61430	28.821	229	145	6	35	247	49	275	2.21e-18	83.2
MS.gene065973.t1	AT3G61430	28.821	229	145	6	35	247	49	275	2.21e-18	83.2
MS.gene065973.t1	AT1G01620	28.139	231	145	5	35	247	49	276	6.45e-18	81.6
MS.gene065973.t1	AT3G47440	29.032	217	144	3	37	245	22	236	8.97e-18	80.9
MS.gene065973.t1	AT5G60660	25.746	268	161	6	15	247	5	269	1.29e-17	80.9
MS.gene065973.t1	AT4G00430	28.139	231	145	5	35	247	50	277	1.32e-17	80.9
MS.gene065973.t1	AT4G23400	27.706	231	146	5	35	247	50	277	2.72e-17	80.1
MS.gene065973.t1	AT3G54820	26.033	242	158	4	27	247	27	268	1.46e-16	78.2
MS.gene065973.t1	AT3G53420	25.714	245	155	5	27	247	28	269	3.29e-16	77.0
MS.gene065973.t1	AT3G53420	25.714	245	155	5	27	247	28	269	3.29e-16	77.0
MS.gene065973.t1	AT4G01470	29.046	241	159	4	15	247	1	237	3.40e-16	76.3
MS.gene065973.t1	AT2G45960	28.922	204	131	4	32	222	46	248	6.40e-15	73.2
MS.gene065973.t1	AT2G37170	25.306	245	156	5	27	247	69	310	7.68e-15	73.6
MS.gene065973.t1	AT2G37170	25.306	245	156	5	27	247	26	267	7.81e-15	73.2
MS.gene065973.t1	AT2G45960	28.922	204	131	4	32	222	46	248	8.06e-15	73.2
MS.gene065973.t1	AT1G01620	28.718	195	121	4	68	247	13	204	1.39e-14	71.2
MS.gene065973.t1	AT2G37180	25.306	245	156	5	27	247	26	267	3.70e-14	71.2

Find 61 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACCTTATGTTTATCATATC+TGG	0.173723	4.3:-13327500	MS.gene065973:CDS
TTCCTGGAATTTCAATTGTT+TGG	0.217869	4.3:-13328273	MS.gene065973:CDS
TCCTGGAATTTCAATTGTTT+GGG	0.246530	4.3:-13328272	MS.gene065973:CDS
TACTTGGTGTCTCCTATTCT+TGG	0.280405	4.3:-13326496	MS.gene065973:CDS
CTGTTTCAAGGGAAATCTTT+TGG	0.298217	4.3:+13328162	None:intergenic
GAAAATGTTGTAACACTTCC+TGG	0.313477	4.3:-13328289	MS.gene065973:CDS
TTCATTGATGCTCCTGTGAT+TGG	0.328945	4.3:+13326577	None:intergenic
ACATATTTCTTGATATTTGC+TGG	0.333245	4.3:-13328343	MS.gene065973:CDS
TACTCTCTTGGTCACATCTC+TGG	0.349138	4.3:-13328226	MS.gene065973:CDS
GCAGGTCCTATACTTCTTGC+TGG	0.362295	4.3:+13326553	None:intergenic
ATGGTGCTGGTTTACTCTCT+TGG	0.376622	4.3:-13328238	MS.gene065973:CDS
CCCCAAACAATTGAAATTCC+AGG	0.378868	4.3:+13328271	None:intergenic
CACTGCTTCTTTCTTGCAGA+AGG	0.380717	4.3:-13328540	MS.gene065973:intron
GGCAAACATAATCAGTTTGT+TGG	0.384708	4.3:-13327581	MS.gene065973:CDS
TTGGAGCTGTGGCTGGTGCA+TGG	0.386493	4.3:-13326477	MS.gene065973:CDS
TACGTTTAAAACAATCTCAT+TGG	0.388236	4.3:+13328607	None:intergenic
CAAAAGATTTCCCTTGAAAC+AGG	0.391841	4.3:-13328161	MS.gene065973:intron
GGGACTTGCTGTGATGGTGC+TGG	0.409165	4.3:-13328251	MS.gene065973:CDS
ACTCTTAGACTAATATTTAA+CGG	0.412369	4.3:-13327602	MS.gene065973:CDS
CCTATTCTTGGAGCTGTGGC+TGG	0.421647	4.3:-13326484	MS.gene065973:CDS
TGGAGCTGTGGCTGGTGCAT+GGG	0.426749	4.3:-13326476	MS.gene065973:CDS
TTTGTTGGAACACTTCCAGC+TGG	0.433109	4.3:-13327566	MS.gene065973:CDS
CCAGCCACAGCTCCAAGAAT+AGG	0.454788	4.3:+13326484	None:intergenic
GAGTTTGGTCACCTGTTTCA+AGG	0.455962	4.3:+13328150	None:intergenic
ACAGTGAATACAGAGGAATA+TGG	0.460120	4.3:-13326522	MS.gene065973:CDS
ATGAATCCAGCAAGAAGTAT+AGG	0.469904	4.3:-13326559	MS.gene065973:CDS
ATTCAATCACAAAAGCTTGA+AGG	0.482940	4.3:+13327537	None:intergenic
TGCAGATTGGTGAGTTGGCT+GGG	0.483568	4.3:-13327109	MS.gene065973:intron
TGCTGGTTGTGCTTCAGTGT+TGG	0.486213	4.3:-13328326	MS.gene065973:CDS
TGTTTGGGGACTTGCTGTGA+TGG	0.487027	4.3:-13328257	MS.gene065973:CDS
TTGCAGATTGGTGAGTTGGC+TGG	0.488849	4.3:-13327110	MS.gene065973:intron
CTCTTGGTGATCTCGCGAAC+CGG	0.489371	4.3:+13326424	None:intergenic
CATGGGTTTATAACGTCATT+CGG	0.494271	4.3:-13326459	MS.gene065973:CDS
CAGTTGGTAGCTGAGGTGAT+AGG	0.497515	4.3:-13328367	MS.gene065973:CDS
CAGAGGAATATGGATATACT+TGG	0.503606	4.3:-13326512	MS.gene065973:CDS
CTCCAGATATGATAAACATA+AGG	0.504080	4.3:+13327498	None:intergenic
AAACTTGCAGATTGGTGAGT+TGG	0.505757	4.3:-13327114	MS.gene065973:intron
GAAAGAAGCAGTGGCATGAG+AGG	0.513919	4.3:+13328550	None:intergenic
TATTCACTGTGTAGAAAAGC+AGG	0.516811	4.3:+13326535	None:intergenic
GCTTGAAGGTCAGACCCAGC+TGG	0.524734	4.3:+13327551	None:intergenic
TTGTTGGAACACTTCCAGCT+GGG	0.527294	4.3:-13327565	MS.gene065973:CDS
CCTAAAAGCAGCAAAACCAT+AGG	0.538587	4.3:-13326392	None:intergenic
CCTGGAATTTCAATTGTTTG+GGG	0.538744	4.3:-13328271	MS.gene065973:CDS
GAGTTGGCTGGGATTGCCGT+TGG	0.544203	4.3:-13327098	MS.gene065973:CDS
GTCTCCTATTCTTGGAGCTG+TGG	0.546485	4.3:-13326488	MS.gene065973:CDS
AAGAGTTCCACTTGCAAGTG+TGG	0.546686	4.3:+13327619	None:intergenic
TTTGGTGCTGGCAAAAGCAA+TGG	0.546727	4.3:+13328180	None:intergenic
CATTCGGTACACGGATAAGC+CGG	0.557498	4.3:-13326443	MS.gene065973:CDS
AGTTTGGTCACCTGTTTCAA+GGG	0.560701	4.3:+13328151	None:intergenic
ACTTGAGCTGCTACATAAGC+CGG	0.561215	4.3:+13327650	None:intergenic
GCAAAAGCAATGGTAACTGC+AGG	0.561982	4.3:+13328190	None:intergenic
ATTTATGCAGTTGGTAGCTG+AGG	0.567762	4.3:-13328374	MS.gene065973:intron
TTTGGATCCACACTTGCAAG+TGG	0.573697	4.3:-13327626	MS.gene065973:CDS
CTAAAAGCAGCAAAACCATA+GGG	0.583342	4.3:-13326391	None:intergenic
TTATAACGTCATTCGGTACA+CGG	0.586612	4.3:-13326452	MS.gene065973:CDS
TTATATGACAGACCAATCAC+AGG	0.604024	4.3:-13326589	MS.gene065973:intron
CAAAAGTACAGTAGATCCAA+CGG	0.621362	4.3:+13327082	None:intergenic
CGTTACCGCTCTATTATCAG+TGG	0.661375	4.3:+13327472	None:intergenic
CTTCTGCAAGAAAGAAGCAG+TGG	0.662046	4.3:+13328541	None:intergenic
TTTCTACACAGTGAATACAG+AGG	0.725695	4.3:-13326529	MS.gene065973:CDS
AGTTGCCACTGATAATAGAG+CGG	0.742875	4.3:-13327477	MS.gene065973:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATTTTTTATTTTTTTTAAA+TGG	+	chr4.3:13328153-13328172	None:intergenic	0.0%
!!	ATATTATAAACTAATATGAA+AGG	+	chr4.3:13327016-13327035	None:intergenic	10.0%
!!!	ATGATAATATTGAATAATTT+TGG	+	chr4.3:13328002-13328021	None:intergenic	10.0%
!!!	TGATAATATTGAATAATTTT+GGG	+	chr4.3:13328001-13328020	None:intergenic	10.0%
!!	AAATATGATTGATTATCATT+TGG	+	chr4.3:13328290-13328309	None:intergenic	15.0%
!!	AGGGAATAAAATAAAAAATT+GGG	-	chr4.3:13327089-13327108	MS.gene065973:CDS	15.0%
!!	GAATTACAAATATTCAAATA+TGG	+	chr4.3:13327849-13327868	None:intergenic	15.0%
!!	TAGGGAATAAAATAAAAAAT+TGG	-	chr4.3:13327088-13327107	MS.gene065973:CDS	15.0%
!!!	TTTGCTATTTGATTTATTAA+AGG	-	chr4.3:13326574-13326593	MS.gene065973:CDS	15.0%
!!	AAAACACAATTTCATTGAAT+AGG	-	chr4.3:13327069-13327088	MS.gene065973:CDS	20.0%
!!	AAACACAATTTCATTGAATA+GGG	-	chr4.3:13327070-13327089	MS.gene065973:CDS	20.0%
!!	AATAATTGACATGATTTCTT+TGG	+	chr4.3:13327039-13327058	None:intergenic	20.0%
!!	AATCTTTCCATTTATTGTAT+AGG	-	chr4.3:13327353-13327372	MS.gene065973:intron	20.0%
!!	ACTCTTAGACTAATATTTAA+CGG	-	chr4.3:13327426-13327445	MS.gene065973:intron	20.0%
!!	ATAAAAGCCAATAAATAGAA+TGG	+	chr4.3:13327877-13327896	None:intergenic	20.0%
!!	TCAAACTCTAATATAAAAAG+AGG	-	chr4.3:13327711-13327730	MS.gene065973:intron	20.0%
!!	TGTGATTTAGTATTTATTCT+TGG	-	chr4.3:13328078-13328097	MS.gene065973:intron	20.0%
!!!	AATCTGAAACTTTTTTTTCA+TGG	-	chr4.3:13326620-13326639	MS.gene065973:intron	20.0%
!!!	AATTTCATCTCTCTTTATTT+TGG	-	chr4.3:13327631-13327650	MS.gene065973:CDS	20.0%
!	AATACGTGTTTAGCAAAATA+GGG	-	chr4.3:13327672-13327691	MS.gene065973:intron	25.0%
!	ACATATTTCTTGATATTTGC+TGG	-	chr4.3:13326685-13326704	MS.gene065973:intron	25.0%
!	AGATTTATAGATAGTTAGCA+AGG	+	chr4.3:13327338-13327357	None:intergenic	25.0%
!	ATACTATATGTTTCTCTTCA+TGG	-	chr4.3:13328104-13328123	MS.gene065973:intron	25.0%
!	GTTGTCTAAATATAGTTGTA+AGG	-	chr4.3:13327749-13327768	MS.gene065973:intron	25.0%
!	TAATACGTGTTTAGCAAAAT+AGG	-	chr4.3:13327671-13327690	MS.gene065973:intron	25.0%
!	TACCTTATGTTTATCATATC+TGG	-	chr4.3:13327528-13327547	MS.gene065973:CDS	25.0%
!	TAGAAATTTGCAGAATCATA+AGG	+	chr4.3:13328406-13328425	None:intergenic	25.0%
!	TCTTAGACCATTCTATTTAT+TGG	-	chr4.3:13327867-13327886	MS.gene065973:intron	25.0%
!	TTTGCCAAATAGTTGAAAAA+TGG	+	chr4.3:13326905-13326924	None:intergenic	25.0%
!!	CTCAACCAGATTTTAAATAA+AGG	-	chr4.3:13327163-13327182	MS.gene065973:intron	25.0%
!!	GCAAACCTTTATTTAAAATC+TGG	+	chr4.3:13327171-13327190	None:intergenic	25.0%
!!	TACGTTTAAAACAATCTCAT+TGG	+	chr4.3:13326424-13326443	None:intergenic	25.0%
!!	TTGTTTCTTGCTTGTTAATT+TGG	-	chr4.3:13327599-13327618	MS.gene065973:CDS	25.0%
!!!	AAAACTTGTTAGGAAATTAG+AGG	-	chr4.3:13326922-13326941	MS.gene065973:intron	25.0%
!!!	ATTCATTTTTCAAGCAAAGT+AGG	+	chr4.3:13326971-13326990	None:intergenic	25.0%
!!!	GATTGTTTTAAACGTAAACA+AGG	-	chr4.3:13326428-13326447	MS.gene065973:CDS	25.0%
!!!	TTATTTTGAAACTTGCAGAT+TGG	-	chr4.3:13327906-13327925	MS.gene065973:intron	25.0%
!!!	TTGTAAGGTTTTGAATCATT+TGG	-	chr4.3:13327764-13327783	MS.gene065973:intron	25.0%
	ACTGTTAAATACGATCAATG+CGG	+	chr4.3:13327263-13327282	None:intergenic	30.0%
	ATACGTGTTTAGCAAAATAG+GGG	-	chr4.3:13327673-13327692	MS.gene065973:intron	30.0%
	ATTCAATCACAAAAGCTTGA+AGG	+	chr4.3:13327494-13327513	None:intergenic	30.0%
	ATTCTCCATAATGTCAAGAA+CGG	-	chr4.3:13327284-13327303	MS.gene065973:intron	30.0%
	CTCCAGATATGATAAACATA+AGG	+	chr4.3:13327533-13327552	None:intergenic	30.0%
	TAGTTGAAAAATGGTGAGTT+TGG	+	chr4.3:13326896-13326915	None:intergenic	30.0%
	TCCATTTATTGTATAGGTAC+CGG	-	chr4.3:13327359-13327378	MS.gene065973:intron	30.0%
	TCCTGGAATTTCAATTGTTT+GGG	-	chr4.3:13326756-13326775	MS.gene065973:intron	30.0%
	TGTGACAATATTTATGCAGT+TGG	-	chr4.3:13326645-13326664	MS.gene065973:intron	30.0%
	TTCCTGGAATTTCAATTGTT+TGG	-	chr4.3:13326755-13326774	MS.gene065973:intron	30.0%
	TTGAGACAAAAAGAACGAAA+CGG	+	chr4.3:13326519-13326538	None:intergenic	30.0%
!	ACTATTTGGCAAAACTTGTT+AGG	-	chr4.3:13326912-13326931	MS.gene065973:intron	30.0%
!	TTTTGAATGTGATGTTTGCA+GGG	-	chr4.3:13327964-13327983	MS.gene065973:intron	30.0%
!!	CTCACCATTTTTCAACTATT+TGG	-	chr4.3:13326898-13326917	MS.gene065973:intron	30.0%
!!!	GTTAGCAAGGTTTTAAATAG+AGG	+	chr4.3:13327325-13327344	None:intergenic	30.0%
	ACAGTGAATACAGAGGAATA+TGG	-	chr4.3:13328506-13328525	MS.gene065973:intron	35.0%
	CAAAAGATTTCCCTTGAAAC+AGG	-	chr4.3:13326867-13326886	MS.gene065973:intron	35.0%
	CAGAGGAATATGGATATACT+TGG	-	chr4.3:13328516-13328535	MS.gene065973:intron	35.0%
	CATGGGTTTATAACGTCATT+CGG	-	chr4.3:13328569-13328588	MS.gene065973:CDS	35.0%
	CCTGGAATTTCAATTGTTTG+GGG	-	chr4.3:13326757-13326776	MS.gene065973:intron	35.0%
	GAAAATGTTGTAACACTTCC+TGG	-	chr4.3:13326739-13326758	MS.gene065973:intron	35.0%
	GGCAAACATAATCAGTTTGT+TGG	-	chr4.3:13327447-13327466	MS.gene065973:intron	35.0%
	TATTCACTGTGTAGAAAAGC+AGG	+	chr4.3:13328496-13328515	None:intergenic	35.0%
	TTATAACGTCATTCGGTACA+CGG	-	chr4.3:13328576-13328595	MS.gene065973:CDS	35.0%
	TTATATGACAGACCAATCAC+AGG	-	chr4.3:13328439-13328458	MS.gene065973:intron	35.0%
	TTTCTACACAGTGAATACAG+AGG	-	chr4.3:13328499-13328518	MS.gene065973:intron	35.0%
!	ATGAATCCAGCAAGAAGTAT+AGG	-	chr4.3:13328469-13328488	MS.gene065973:intron	35.0%
!	CAAAAGTACAGTAGATCCAA+CGG	+	chr4.3:13327949-13327968	None:intergenic	35.0%
!	CAAGCAAGAAACAAAGCATT+AGG	+	chr4.3:13327593-13327612	None:intergenic	35.0%
!	CTGTTTCAAGGGAAATCTTT+TGG	+	chr4.3:13326869-13326888	None:intergenic	35.0%
!	CTTTTGAATGTGATGTTTGC+AGG	-	chr4.3:13327963-13327982	MS.gene065973:intron	35.0%
!	TATGTAGCAGCTCAAGTTTT+TGG	-	chr4.3:13327384-13327403	MS.gene065973:intron	35.0%
!	TTACGTAGTTCATCACCTTT+TGG	-	chr4.3:13328026-13328045	MS.gene065973:intron	35.0%
!!	ACTTGCTATCTGTTTCCAAA+AGG	+	chr4.3:13328044-13328063	None:intergenic	35.0%
!!	TTTTAGGAAAGATGAGCTCT+TGG	+	chr4.3:13328623-13328642	None:intergenic	35.0%
	AAACTTGCAGATTGGTGAGT+TGG	-	chr4.3:13327914-13327933	MS.gene065973:intron	40.0%
	AGTTGCCACTGATAATAGAG+CGG	-	chr4.3:13327551-13327570	MS.gene065973:CDS	40.0%
	AGTTTGGTCACCTGTTTCAA+GGG	+	chr4.3:13326880-13326899	None:intergenic	40.0%
	CCCCAAACAATTGAAATTCC+AGG	+	chr4.3:13326760-13326779	None:intergenic	40.0%
	CTGCAACATGATTGCAATTG+AGG	-	chr4.3:13327237-13327256	MS.gene065973:intron	40.0%
	GCCGGTACCTATACAATAAA+TGG	+	chr4.3:13327363-13327382	None:intergenic	40.0%
	GTGTTTAGCAAAATAGGGGT+TGG	-	chr4.3:13327677-13327696	MS.gene065973:intron	40.0%
	TTCATTGATGCTCCTGTGAT+TGG	+	chr4.3:13328454-13328473	None:intergenic	40.0%
!!	ATTTATGCAGTTGGTAGCTG+AGG	-	chr4.3:13326654-13326673	MS.gene065973:intron	40.0%
!!	TACTTGGTGTCTCCTATTCT+TGG	-	chr4.3:13328532-13328551	MS.gene065973:intron	40.0%
	AAGAGTTCCACTTGCAAGTG+TGG	+	chr4.3:13327412-13327431	None:intergenic	45.0%
	ACTTGAGCTGCTACATAAGC+CGG	+	chr4.3:13327381-13327400	None:intergenic	45.0%
	CACTGCTTCTTTCTTGCAGA+AGG	-	chr4.3:13326488-13326507	MS.gene065973:CDS	45.0%
	CGTTACCGCTCTATTATCAG+TGG	+	chr4.3:13327559-13327578	None:intergenic	45.0%
	CTTCTGCAAGAAAGAAGCAG+TGG	+	chr4.3:13326490-13326509	None:intergenic	45.0%
	GAGTTTGGTCACCTGTTTCA+AGG	+	chr4.3:13326881-13326900	None:intergenic	45.0%
	GCAAAAGCAATGGTAACTGC+AGG	+	chr4.3:13326841-13326860	None:intergenic	45.0%
	TACTCTCTTGGTCACATCTC+TGG	-	chr4.3:13326802-13326821	MS.gene065973:intron	45.0%
	TTATGTCGCCACCAAGATCA+CGG	+	chr4.3:13327196-13327215	None:intergenic	45.0%
	TTGTTGGAACACTTCCAGCT+GGG	-	chr4.3:13327463-13327482	MS.gene065973:intron	45.0%
	TTTGGATCCACACTTGCAAG+TGG	-	chr4.3:13327402-13327421	MS.gene065973:intron	45.0%
	TTTGTTGGAACACTTCCAGC+TGG	-	chr4.3:13327462-13327481	MS.gene065973:intron	45.0%
!	GAGTGCCGTTCTTGACATTA+TGG	+	chr4.3:13327292-13327311	None:intergenic	45.0%
!!	ATGGTGCTGGTTTACTCTCT+TGG	-	chr4.3:13326790-13326809	MS.gene065973:intron	45.0%
!!	CAAGGGAAATCTTTTGGTGC+TGG	+	chr4.3:13326863-13326882	None:intergenic	45.0%
!!	TTTGGTGCTGGCAAAAGCAA+TGG	+	chr4.3:13326851-13326870	None:intergenic	45.0%
	AAGGTTTGCGACCGTGATCT+TGG	-	chr4.3:13327182-13327201	MS.gene065973:intron	50.0%
	CATTCGGTACACGGATAAGC+CGG	-	chr4.3:13328585-13328604	MS.gene065973:CDS	50.0%
	GAAAGAAGCAGTGGCATGAG+AGG	+	chr4.3:13326481-13326500	None:intergenic	50.0%
	GCAGGTCCTATACTTCTTGC+TGG	+	chr4.3:13328478-13328497	None:intergenic	50.0%
	GTCTCCTATTCTTGGAGCTG+TGG	-	chr4.3:13328540-13328559	MS.gene065973:intron	50.0%
!	TGCAGATTGGTGAGTTGGCT+GGG	-	chr4.3:13327919-13327938	MS.gene065973:intron	50.0%
!	TGCTGGTTGTGCTTCAGTGT+TGG	-	chr4.3:13326702-13326721	MS.gene065973:intron	50.0%
!	TTGCAGATTGGTGAGTTGGC+TGG	-	chr4.3:13327918-13327937	MS.gene065973:intron	50.0%
!!	CAGTTGGTAGCTGAGGTGAT+AGG	-	chr4.3:13326661-13326680	MS.gene065973:intron	50.0%
!!	TGTTTGGGGACTTGCTGTGA+TGG	-	chr4.3:13326771-13326790	MS.gene065973:intron	50.0%
	CCAGCCACAGCTCCAAGAAT+AGG	+	chr4.3:13328547-13328566	None:intergenic	55.0%
	CCTATTCTTGGAGCTGTGGC+TGG	-	chr4.3:13328544-13328563	MS.gene065973:CDS	55.0%
	GTTTGCGACCGTGATCTTGG+TGG	-	chr4.3:13327185-13327204	MS.gene065973:intron	55.0%
!	CTCTTGGTGATCTCGCGAAC+CGG	+	chr4.3:13328607-13328626	None:intergenic	55.0%
	GAGTTGGCTGGGATTGCCGT+TGG	-	chr4.3:13327930-13327949	MS.gene065973:intron	60.0%
	GCTTGAAGGTCAGACCCAGC+TGG	+	chr4.3:13327480-13327499	None:intergenic	60.0%
	TGGAGCTGTGGCTGGTGCAT+GGG	-	chr4.3:13328552-13328571	MS.gene065973:CDS	60.0%
	TTGGAGCTGTGGCTGGTGCA+TGG	-	chr4.3:13328551-13328570	MS.gene065973:CDS	60.0%
!!	GGGACTTGCTGTGATGGTGC+TGG	-	chr4.3:13326777-13326796	MS.gene065973:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	13326393	13328657	13326393	ID=MS.gene065973
chr4.3	mRNA	13326393	13328657	13326393	ID=MS.gene065973.t1;Parent=MS.gene065973
chr4.3	exon	13328541	13328657	13328541	ID=MS.gene065973.t1.exon1;Parent=MS.gene065973.t1
chr4.3	CDS	13328541	13328657	13328541	ID=cds.MS.gene065973.t1;Parent=MS.gene065973.t1
chr4.3	exon	13328162	13328386	13328162	ID=MS.gene065973.t1.exon2;Parent=MS.gene065973.t1
chr4.3	CDS	13328162	13328386	13328162	ID=cds.MS.gene065973.t1;Parent=MS.gene065973.t1
chr4.3	exon	13327478	13327675	13327478	ID=MS.gene065973.t1.exon3;Parent=MS.gene065973.t1
chr4.3	CDS	13327478	13327675	13327478	ID=cds.MS.gene065973.t1;Parent=MS.gene065973.t1
chr4.3	exon	13327065	13327126	13327065	ID=MS.gene065973.t1.exon4;Parent=MS.gene065973.t1
chr4.3	CDS	13327065	13327126	13327065	ID=cds.MS.gene065973.t1;Parent=MS.gene065973.t1
chr4.3	exon	13326393	13326600	13326393	ID=MS.gene065973.t1.exon5;Parent=MS.gene065973.t1
chr4.3	CDS	13326393	13326600	13326393	ID=cds.MS.gene065973.t1;Parent=MS.gene065973.t1

Gene Sequence

>MS.gene065973

ATGGAAGAAAATTCAGCTAGCAATGCAACCAATGAGATTGTTTTAAACGTAAACAAGGCTGTCTCTAACACAAGTGAAGACTCAACCTCTCATGCCACTGCTTCTTTCTTGCAGAAGGTGCATCCGTTTCGTTCTTTTTGTCTCAAAAACAAGTTCAAATGTTTTATTTGTTCATAACTAATTTGCTATTTGATTTATTAAAGGTTTTGTTATGTTATGTCTACTAAAATCTGAAACTTTTTTTTCATGGATTGTGACAATATTTATGCAGTTGGTAGCTGAGGTGATAGGCACATATTTCTTGATATTTGCTGGTTGTGCTTCAGTGTTGGTGAACAAGAACAATGAAAATGTTGTAACACTTCCTGGAATTTCAATTGTTTGGGGACTTGCTGTGATGGTGCTGGTTTACTCTCTTGGTCACATCTCTGGTGCTCATTTCAATCCTGCAGTTACCATTGCTTTTGCCAGCACCAAAAGATTTCCCTTGAAACAGGTGACCAAACTCACCATTTTTCAACTATTTGGCAAAACTTGTTAGGAAATTAGAGGTCAAATTAGTTCATGATCTACAGCCTACTTTGCTTGAAAAATGAATTTCTTCTTCTTACTTGTTTTGACCTTTCATATTAGTTTATAATATCCAAAGAAATCATGTCAATTATTGCACATTGTGAAAACACAATTTCATTGAATAGGGAATAAAATAAAAAATTGGGTGTTCACACACACGTAGATAGAAAAATATTGTAATGAAAGATAACTTATCTCTCAACCAGATTTTAAATAAAGGTTTGCGACCGTGATCTTGGTGGCGACATAATAATTTTTTATGTTGTCGACACTGCAACATGATTGCAATTGAGGCCGCATTGATCGTATTTAACAGTGATTCTCCATAATGTCAAGAACGGCACTCGTCACTGCGACCTCTATTTAAAACCTTGCTAACTATCTATAAATCTTTCCATTTATTGTATAGGTACCGGCTTATGTAGCAGCTCAAGTTTTTGGATCCACACTTGCAAGTGGAACTCTTAGACTAATATTTAACGGCAAACATAATCAGTTTGTTGGAACACTTCCAGCTGGGTCTGACCTTCAAGCTTTTGTGATTGAATTCATCATCACTTTTTACCTTATGTTTATCATATCTGGAGTTGCCACTGATAATAGAGCGGTAACGTGTTTGTTCATCCTAATGCTTTGTTTCTTGCTTGTTAATTTGGTTATTAACTAATTTCATCTCTCTTTATTTTGGTATAAAGTATTTTGATTTAATACGTGTTTAGCAAAATAGGGGTTGGCTAATCATCTTTCAAACTCTAATATAAAAAGAGGCAATTAAATTGACTAGTTGTCTAAATATAGTTGTAAGGTTTTGAATCATTTGGAAGAATGCTAGCTGCTAGTAGTTTACATAACAAGACAAAGCTTATACGAAACAAAACGACCATATTTGAATATTTGTAATTCTTAGACCATTCTATTTATTGGCTTTTATTTATTCGTTTTATTTTGAAACTTGCAGATTGGTGAGTTGGCTGGGATTGCCGTTGGATCTACTGTACTTTTGAATGTGATGTTTGCAGGGTATTAACTTATCCCAAAATTATTCAATATTATCATTTGCTTACGTAGTTCATCACCTTTTGGAAACAGATAGCAAGTCATGATCTCATTTGTGTGATTTAGTATTTATTCTTGGAGCATACTATATGTTTCTCTTCATGGTTAAGACTTAAGATCATGTGATGCCATTTAAAAAAAATAAAAAATAATTAATAAATCATGTGATGCAGTAAAAACTTAGAATAGAACACTGTATTATGTTACTTGCTGAGTTTAATTTTGATACACTTTTAGTGTAAAATGTTTTATTCTATCAGTGTCACCAAATGATAATCAATCATATTTTTTACTTTAGAAACTTACGATAAAAATCAAACGCTTTTAATAATCTAACGATTATGATTGATCATTTATTCTAAAAGTGTATATGAAGTAAATCCTTATGATTCTGCAAATTTCTAATGACATTATTTCTTATATGACAGACCAATCACAGGAGCATCAATGAATCCAGCAAGAAGTATAGGACCTGCTTTTCTACACAGTGAATACAGAGGAATATGGATATACTTGGTGTCTCCTATTCTTGGAGCTGTGGCTGGTGCATGGGTTTATAACGTCATTCGGTACACGGATAAGCCGGTTCGCGAGATCACCAAGAGCTCATCTTTCCTAAAAGCAGCAAAACCATAG

Protein sequence

>MS.gene065973.t1

MEENSASNATNEIVLNVNKAVSNTSEDSTSHATASFLQKLVAEVIGTYFLIFAGCASVLVNKNNENVVTLPGISIVWGLAVMVLVYSLGHISGAHFNPAVTIAFASTKRFPLKQVPAYVAAQVFGSTLASGTLRLIFNGKHNQFVGTLPAGSDLQAFVIEFIITFYLMFIISGVATDNRAIGELAGIAVGSTVLLNVMFAGPITGASMNPARSIGPAFLHSEYRGIWIYLVSPILGAVAGAWVYNVIRYTDKPVREITKSSSFLKAAKP